TY  - JOUR
AU  - Mačvanin, Mirjana
AU  - Gluvić, Zoran
AU  - Bajić, Vladan
AU  - Isenović, Esma R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11574
AB  - Diabetes mellitus (DM) is a group of metabolic disorders defined by hyperglycemia induced by insulin resistance, inadequate insulin secretion, or excessive glucagon secretion. In 2021, the global prevalence of diabetes is anticipated to be 10.7% (537 million people). Noncoding RNAs (ncRNAs) appear to have an important role in the initiation and progression of DM, according to a growing body of research. The two major groups of ncRNAs implicated in diabetic disorders are miRNAs and long noncoding RNAs. miRNAs are singlestranded, short (17–25 nucleotides), ncRNAs that influence gene expression at the post-transcriptional level. Because DM has reached epidemic proportions worldwide, it appears that novel diagnostic and therapeutic strategies are required to identify and treat complications associated with these diseases efficiently. miRNAs are gaining attention as biomarkers for DM diagnosis and potential treatment due to their function in maintaining physiological homeostasis via gene expression regulation. In this review, we address the issue of the gradually expanding global prevalence of DM by presenting a complete and upto-date synopsis of various regulatory miRNAs involved in these disorders. We hope this review will spark discussion about ncRNAs as prognostic biomarkers and therapeutic tools for DM. We examine and synthesize recent research that used novel, high-throughput technologies to uncover ncRNAs involved in DM, necessitating a systematic approach to examining and summarizing their roles and possible diagnostic and therapeutic uses.
T2  - World Journal of Diabetes
T1  - Novel insights regarding the role of noncoding RNAs in diabetes
VL  - 14
IS  - 7
SP  - 958
EP  - 976
DO  - 10.4239/wjd.v14.i7.958
ER  - 

@article{
author = "Mačvanin, Mirjana and Gluvić, Zoran and Bajić, Vladan and Isenović, Esma R.",
year = "2023",
abstract = "Diabetes mellitus (DM) is a group of metabolic disorders defined by hyperglycemia induced by insulin resistance, inadequate insulin secretion, or excessive glucagon secretion. In 2021, the global prevalence of diabetes is anticipated to be 10.7% (537 million people). Noncoding RNAs (ncRNAs) appear to have an important role in the initiation and progression of DM, according to a growing body of research. The two major groups of ncRNAs implicated in diabetic disorders are miRNAs and long noncoding RNAs. miRNAs are singlestranded, short (17–25 nucleotides), ncRNAs that influence gene expression at the post-transcriptional level. Because DM has reached epidemic proportions worldwide, it appears that novel diagnostic and therapeutic strategies are required to identify and treat complications associated with these diseases efficiently. miRNAs are gaining attention as biomarkers for DM diagnosis and potential treatment due to their function in maintaining physiological homeostasis via gene expression regulation. In this review, we address the issue of the gradually expanding global prevalence of DM by presenting a complete and upto-date synopsis of various regulatory miRNAs involved in these disorders. We hope this review will spark discussion about ncRNAs as prognostic biomarkers and therapeutic tools for DM. We examine and synthesize recent research that used novel, high-throughput technologies to uncover ncRNAs involved in DM, necessitating a systematic approach to examining and summarizing their roles and possible diagnostic and therapeutic uses.",
journal = "World Journal of Diabetes",
title = "Novel insights regarding the role of noncoding RNAs in diabetes",
volume = "14",
number = "7",
pages = "958-976",
doi = "10.4239/wjd.v14.i7.958"
}

Mačvanin, M., Gluvić, Z., Bajić, V.,& Isenović, E. R.. (2023). Novel insights regarding the role of noncoding RNAs in diabetes. in World Journal of Diabetes, 14(7), 958-976.
https://doi.org/10.4239/wjd.v14.i7.958

Mačvanin M, Gluvić Z, Bajić V, Isenović ER. Novel insights regarding the role of noncoding RNAs in diabetes. in World Journal of Diabetes. 2023;14(7):958-976.
doi:10.4239/wjd.v14.i7.958 .

Mačvanin, Mirjana, Gluvić, Zoran, Bajić, Vladan, Isenović, Esma R., "Novel insights regarding the role of noncoding RNAs in diabetes" in World Journal of Diabetes, 14, no. 7 (2023):958-976,
https://doi.org/10.4239/wjd.v14.i7.958 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Essack, Magbubah
AU  - Šami, Ahmad
AU  - Perry, George
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Bajić, Vladan P.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11351
AB  - MicroRNAs (miRNAs) are involved in the regulation of various cellular processes including pathological conditions. MiRNA networks have been extensively researched in age-related degenerative diseases, such as cancer, Alzheimer’s disease (AD), and heart failure. Thus, miRNA has been studied from different approaches, in vivo, in vitro, and in silico including miRNA networks. Networks linking diverse biomedical entities unveil information not readily observable by other means. This work focuses on biological networks related to Breast cancer susceptibility 1 (BRCA1) in AD and breast cancer (BC). Using various bioinformatics approaches, we identified subnetworks common to AD and BC that suggest they are linked. According to our results, miR-107 was identified as a potentially good candidate for both AD and BC treatment (targeting BRCA1/2 and PTEN in both diseases), accompanied by miR-146a and miR-17. The analysis also confirmed the involvement of the miR-17-92 cluster, and miR-124-3p, and highlighted the importance of poorly researched miRNAs such as mir-6785 mir6127, mir-6870, or miR-8485. After filtering the in silico analysis results, we found 49 miRNA molecules that modulate the expression of at least five genes common to both BC and AD. Those 49 miRNAs regulate the expression of 122 genes in AD and 93 genes in BC, from which 26 genes are common genes for AD and BC involved in neuron differentiation and genesis, cell differentiation and migration, regulation of cell cycle, and cancer development. Additionally, the highly enriched pathway was associated with diabetic complications, pointing out possible interplay among molecules underlying BC, AD, and diabetes pathology
T2  - Computational Biology and Chemistry
T1  - MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment
VL  - 106
SP  - 107925
DO  - 10.1016/j.compbiolchem.2023.107925
ER  - 

@article{
author = "Petrović, Nina and Essack, Magbubah and Šami, Ahmad and Perry, George and Gojobori, Takashi and Isenović, Esma R. and Bajić, Vladan P.",
year = "2023",
abstract = "MicroRNAs (miRNAs) are involved in the regulation of various cellular processes including pathological conditions. MiRNA networks have been extensively researched in age-related degenerative diseases, such as cancer, Alzheimer’s disease (AD), and heart failure. Thus, miRNA has been studied from different approaches, in vivo, in vitro, and in silico including miRNA networks. Networks linking diverse biomedical entities unveil information not readily observable by other means. This work focuses on biological networks related to Breast cancer susceptibility 1 (BRCA1) in AD and breast cancer (BC). Using various bioinformatics approaches, we identified subnetworks common to AD and BC that suggest they are linked. According to our results, miR-107 was identified as a potentially good candidate for both AD and BC treatment (targeting BRCA1/2 and PTEN in both diseases), accompanied by miR-146a and miR-17. The analysis also confirmed the involvement of the miR-17-92 cluster, and miR-124-3p, and highlighted the importance of poorly researched miRNAs such as mir-6785 mir6127, mir-6870, or miR-8485. After filtering the in silico analysis results, we found 49 miRNA molecules that modulate the expression of at least five genes common to both BC and AD. Those 49 miRNAs regulate the expression of 122 genes in AD and 93 genes in BC, from which 26 genes are common genes for AD and BC involved in neuron differentiation and genesis, cell differentiation and migration, regulation of cell cycle, and cancer development. Additionally, the highly enriched pathway was associated with diabetic complications, pointing out possible interplay among molecules underlying BC, AD, and diabetes pathology",
journal = "Computational Biology and Chemistry",
title = "MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment",
volume = "106",
pages = "107925",
doi = "10.1016/j.compbiolchem.2023.107925"
}

Petrović, N., Essack, M., Šami, A., Perry, G., Gojobori, T., Isenović, E. R.,& Bajić, V. P.. (2023). MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment. in Computational Biology and Chemistry, 106, 107925.
https://doi.org/10.1016/j.compbiolchem.2023.107925

Petrović N, Essack M, Šami A, Perry G, Gojobori T, Isenović ER, Bajić VP. MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment. in Computational Biology and Chemistry. 2023;106:107925.
doi:10.1016/j.compbiolchem.2023.107925 .

Petrović, Nina, Essack, Magbubah, Šami, Ahmad, Perry, George, Gojobori, Takashi, Isenović, Esma R., Bajić, Vladan P., "MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment" in Computational Biology and Chemistry, 106 (2023):107925,
https://doi.org/10.1016/j.compbiolchem.2023.107925 . .

TY  - JOUR
AU  - Alamro, Hind
AU  - Bajić, Vladan P.
AU  - Mačvanin, Mirjana
AU  - Isenović, Esma R.
AU  - Gojobori, Takashi
AU  - Essack, Magbubah
AU  - Gao, Xin
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10628
AB  - MicroRNAs (miRNAs) are critical regulators of gene expression in healthy and diseased states, and numerous studies have established their tremendous potential as a tool for improving the diagnosis of Type 2 Diabetes Mellitus (T2D) and its comorbidities. In this regard, we computationally identify novel top-ranked hub miRNAs that might be involved in T2D. We accomplish this via two strategies: 1) by ranking miRNAs based on the number of T2D differentially expressed genes (DEGs) they target, and 2) using only the common DEGs between T2D and its comorbidity, Alzheimer’s disease (AD) to predict and rank miRNA. Then classifier models are built using the DEGs targeted by each miRNA as features. Here, we show the T2D DEGs targeted by hsa-mir-1-3p, hsa-mir-16-5p, hsa-mir-124-3p, hsa-mir-34a-5p, hsa-let-7b-5p, hsa-mir-155-5p, hsa-mir-107, hsa-mir-27a-3p, hsa-mir-129-2-3p, and hsa-mir-146a-5p are capable of distinguishing T2D samples from the controls, which serves as a measure of confidence in the miRNAs’ potential role in T2D progression. Moreover, for the second strategy, we show other critical miRNAs can be made apparent through the disease’s comorbidities, and in this case, overall, the hsa-mir-103a-3p models work well for all the datasets, especially in T2D, while the hsa-mir-124-3p models achieved the best scores for the AD datasets. To the best of our knowledge, this is the first study that used predicted miRNAs to determine the features that can separate the diseased samples (T2D or AD) from the normal ones, instead of using conventional non-biology-based feature selection methods.
T2  - Frontiers in Endocrinology
T1  - Type 2 Diabetes Mellitus and its comorbidity, Alzheimer’s disease: Identifying critical microRNA using machine learning
VL  - 13
SP  - 1084656
DO  - 10.3389/fendo.2022.1084656
ER  - 

@article{
author = "Alamro, Hind and Bajić, Vladan P. and Mačvanin, Mirjana and Isenović, Esma R. and Gojobori, Takashi and Essack, Magbubah and Gao, Xin",
year = "2023",
abstract = "MicroRNAs (miRNAs) are critical regulators of gene expression in healthy and diseased states, and numerous studies have established their tremendous potential as a tool for improving the diagnosis of Type 2 Diabetes Mellitus (T2D) and its comorbidities. In this regard, we computationally identify novel top-ranked hub miRNAs that might be involved in T2D. We accomplish this via two strategies: 1) by ranking miRNAs based on the number of T2D differentially expressed genes (DEGs) they target, and 2) using only the common DEGs between T2D and its comorbidity, Alzheimer’s disease (AD) to predict and rank miRNA. Then classifier models are built using the DEGs targeted by each miRNA as features. Here, we show the T2D DEGs targeted by hsa-mir-1-3p, hsa-mir-16-5p, hsa-mir-124-3p, hsa-mir-34a-5p, hsa-let-7b-5p, hsa-mir-155-5p, hsa-mir-107, hsa-mir-27a-3p, hsa-mir-129-2-3p, and hsa-mir-146a-5p are capable of distinguishing T2D samples from the controls, which serves as a measure of confidence in the miRNAs’ potential role in T2D progression. Moreover, for the second strategy, we show other critical miRNAs can be made apparent through the disease’s comorbidities, and in this case, overall, the hsa-mir-103a-3p models work well for all the datasets, especially in T2D, while the hsa-mir-124-3p models achieved the best scores for the AD datasets. To the best of our knowledge, this is the first study that used predicted miRNAs to determine the features that can separate the diseased samples (T2D or AD) from the normal ones, instead of using conventional non-biology-based feature selection methods.",
journal = "Frontiers in Endocrinology",
title = "Type 2 Diabetes Mellitus and its comorbidity, Alzheimer’s disease: Identifying critical microRNA using machine learning",
volume = "13",
pages = "1084656",
doi = "10.3389/fendo.2022.1084656"
}

Alamro, H., Bajić, V. P., Mačvanin, M., Isenović, E. R., Gojobori, T., Essack, M.,& Gao, X.. (2023). Type 2 Diabetes Mellitus and its comorbidity, Alzheimer’s disease: Identifying critical microRNA using machine learning. in Frontiers in Endocrinology, 13, 1084656.
https://doi.org/10.3389/fendo.2022.1084656

Alamro H, Bajić VP, Mačvanin M, Isenović ER, Gojobori T, Essack M, Gao X. Type 2 Diabetes Mellitus and its comorbidity, Alzheimer’s disease: Identifying critical microRNA using machine learning. in Frontiers in Endocrinology. 2023;13:1084656.
doi:10.3389/fendo.2022.1084656 .

Alamro, Hind, Bajić, Vladan P., Mačvanin, Mirjana, Isenović, Esma R., Gojobori, Takashi, Essack, Magbubah, Gao, Xin, "Type 2 Diabetes Mellitus and its comorbidity, Alzheimer’s disease: Identifying critical microRNA using machine learning" in Frontiers in Endocrinology, 13 (2023):1084656,
https://doi.org/10.3389/fendo.2022.1084656 . .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Salhi, Adil
AU  - Lakota, Katja
AU  - Radovanović, Aleksandar
AU  - Razali, Rozaimi
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Uludag, Mahmut
AU  - Tifratene, Faroug
AU  - Motwalli, Olaa
AU  - Marchand, Benoit
AU  - Bajić, Vladimir
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Essack, Magbubah
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10387
AB  - More than 30 types of amyloids are linked to close to 50 diseases in humans, the most prom- inent being Alzheimer’s disease (AD). AD is brain-related local amyloidosis, while another amyloidosis, such as AA amyloidosis, tends to be more systemic. Therefore, we need to know more about the biological entities’ influencing these amyloidosis processes. However, there is currently no support system developed specifically to handle this extraordinarily complex and demanding task. To acquire a systematic view of amyloidosis and how this may be relevant to the brain and other organs, we needed a means to explore "amyloid net- work systems" that may underly processes that leads to an amyloid-related disease. In this regard, we developed the DES-Amyloidoses knowledgebase (KB) to obtain fast and rele- vant information regarding the biological network related to amyloid proteins/peptides and amyloid-related diseases. This KB contains information obtained through text and data min- ing of available scientific literature and other public repositories. The information compiled into the DES-Amyloidoses system based on 19 topic-specific dictionaries resulted in 796,409 associations between terms from these dictionaries. Users can explore this infor- mation through various options, including enriched concepts, enriched pairs, and semantic similarity. We show the usefulness of the KB using an example focused on inflammasome- amyloid associations. To our knowledge, this is the only KB dedicated to human amyloid- related diseases derived primarily through literature text mining and complemented by data mining that provides a novel way of exploring information relevant to amyloidoses.
T2  - PLoS ONE
T1  - DES-Amyloidoses “Amyloidoses through thelooking-glass”: A knowledgebase developedfor exploring and linking information relatedto human amyloid-related diseases
VL  - 17
IS  - 7
DO  - 10.1371/journal.pone.0271737
ER  - 

@article{
author = "Bajić, Vladan P. and Salhi, Adil and Lakota, Katja and Radovanović, Aleksandar and Razali, Rozaimi and Živković, Lada and Spremo-Potparević, Biljana and Uludag, Mahmut and Tifratene, Faroug and Motwalli, Olaa and Marchand, Benoit and Bajić, Vladimir and Gojobori, Takashi and Isenović, Esma R. and Essack, Magbubah",
year = "2022",
abstract = "More than 30 types of amyloids are linked to close to 50 diseases in humans, the most prom- inent being Alzheimer’s disease (AD). AD is brain-related local amyloidosis, while another amyloidosis, such as AA amyloidosis, tends to be more systemic. Therefore, we need to know more about the biological entities’ influencing these amyloidosis processes. However, there is currently no support system developed specifically to handle this extraordinarily complex and demanding task. To acquire a systematic view of amyloidosis and how this may be relevant to the brain and other organs, we needed a means to explore "amyloid net- work systems" that may underly processes that leads to an amyloid-related disease. In this regard, we developed the DES-Amyloidoses knowledgebase (KB) to obtain fast and rele- vant information regarding the biological network related to amyloid proteins/peptides and amyloid-related diseases. This KB contains information obtained through text and data min- ing of available scientific literature and other public repositories. The information compiled into the DES-Amyloidoses system based on 19 topic-specific dictionaries resulted in 796,409 associations between terms from these dictionaries. Users can explore this infor- mation through various options, including enriched concepts, enriched pairs, and semantic similarity. We show the usefulness of the KB using an example focused on inflammasome- amyloid associations. To our knowledge, this is the only KB dedicated to human amyloid- related diseases derived primarily through literature text mining and complemented by data mining that provides a novel way of exploring information relevant to amyloidoses.",
journal = "PLoS ONE",
title = "DES-Amyloidoses “Amyloidoses through thelooking-glass”: A knowledgebase developedfor exploring and linking information relatedto human amyloid-related diseases",
volume = "17",
number = "7",
doi = "10.1371/journal.pone.0271737"
}

Bajić, V. P., Salhi, A., Lakota, K., Radovanović, A., Razali, R., Živković, L., Spremo-Potparević, B., Uludag, M., Tifratene, F., Motwalli, O., Marchand, B., Bajić, V., Gojobori, T., Isenović, E. R.,& Essack, M.. (2022). DES-Amyloidoses “Amyloidoses through thelooking-glass”: A knowledgebase developedfor exploring and linking information relatedto human amyloid-related diseases. in PLoS ONE, 17(7).
https://doi.org/10.1371/journal.pone.0271737

Bajić VP, Salhi A, Lakota K, Radovanović A, Razali R, Živković L, Spremo-Potparević B, Uludag M, Tifratene F, Motwalli O, Marchand B, Bajić V, Gojobori T, Isenović ER, Essack M. DES-Amyloidoses “Amyloidoses through thelooking-glass”: A knowledgebase developedfor exploring and linking information relatedto human amyloid-related diseases. in PLoS ONE. 2022;17(7).
doi:10.1371/journal.pone.0271737 .

Bajić, Vladan P., Salhi, Adil, Lakota, Katja, Radovanović, Aleksandar, Razali, Rozaimi, Živković, Lada, Spremo-Potparević, Biljana, Uludag, Mahmut, Tifratene, Faroug, Motwalli, Olaa, Marchand, Benoit, Bajić, Vladimir, Gojobori, Takashi, Isenović, Esma R., Essack, Magbubah, "DES-Amyloidoses “Amyloidoses through thelooking-glass”: A knowledgebase developedfor exploring and linking information relatedto human amyloid-related diseases" in PLoS ONE, 17, no. 7 (2022),
https://doi.org/10.1371/journal.pone.0271737 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan P.
AU  - Čabarkapa‐Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Forbes‐Hernández, Tamara Yuliett
AU  - Zlatković‐Švenda, Mirjana
AU  - Perry, George
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9564
AB  - Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD. © 2021 Wiley Periodicals LLC.
T2  - Journal of Food Biochemistry
T1  - Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease
VL  - 45
IS  - 4
DO  - 10.1111/jfbc.13637
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan P. and Čabarkapa‐Pirković, Andrea and Dekanski, Dragana and Forbes‐Hernández, Tamara Yuliett and Zlatković‐Švenda, Mirjana and Perry, George and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD. © 2021 Wiley Periodicals LLC.",
journal = "Journal of Food Biochemistry",
title = "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease",
volume = "45",
number = "4",
doi = "10.1111/jfbc.13637"
}

Živković, L., Bajić, V. P., Čabarkapa‐Pirković, A., Dekanski, D., Forbes‐Hernández, T. Y., Zlatković‐Švenda, M., Perry, G.,& Spremo-Potparević, B.. (2021). Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry, 45(4).
https://doi.org/10.1111/jfbc.13637

Živković L, Bajić VP, Čabarkapa‐Pirković A, Dekanski D, Forbes‐Hernández TY, Zlatković‐Švenda M, Perry G, Spremo-Potparević B. Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry. 2021;45(4).
doi:10.1111/jfbc.13637 .

Živković, Lada, Bajić, Vladan P., Čabarkapa‐Pirković, Andrea, Dekanski, Dragana, Forbes‐Hernández, Tamara Yuliett, Zlatković‐Švenda, Mirjana, Perry, George, Spremo-Potparević, Biljana, "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease" in Journal of Food Biochemistry, 45, no. 4 (2021),
https://doi.org/10.1111/jfbc.13637 . .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Misić, Nataša
AU  - Stanković, Ivana
AU  - Zarić, Božidarka
AU  - Perry, George
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9901
AB  - Does Alzheimer Disease show a decline in cognitive functions that relate to the awareness of external reality? In this paper, we will propose a perspective that patients with increasing symptoms of AD show a change in the awareness of subjective versus objective representative axis of reality thus consequently move to a more internal like perception of reality. This paradigm shift suggests that new insights into the dynamicity of the conscious representation of reality in the AD brain may give us new clues to the very early signs of memory and self-awareness impairment that originates from, in our view the microtubules. Dialog between Adso and William, in Umberto Eco’s The Name of the Rose, Third Day: Vespers. “But how does it happen,” I said with admiration, “that you were able to solve the mystery of the library looking at it from the outside, and you were unable to solve it when you were inside?” “Thus, God knows the world, because He conceived it in His mind, as if it was from the outside, before it was created, and we do not know its rule, because we live inside it, having found it already made.
T2  - Neuroscience Insights
T1  - Alzheimer’s and Consciousness: How Much Subjectivity Is Objective?
VL  - 16
DO  - 10.1177/26331055211033869
ER  - 

@article{
author = "Bajić, Vladan P. and Misić, Nataša and Stanković, Ivana and Zarić, Božidarka and Perry, George",
year = "2021",
abstract = "Does Alzheimer Disease show a decline in cognitive functions that relate to the awareness of external reality? In this paper, we will propose a perspective that patients with increasing symptoms of AD show a change in the awareness of subjective versus objective representative axis of reality thus consequently move to a more internal like perception of reality. This paradigm shift suggests that new insights into the dynamicity of the conscious representation of reality in the AD brain may give us new clues to the very early signs of memory and self-awareness impairment that originates from, in our view the microtubules. Dialog between Adso and William, in Umberto Eco’s The Name of the Rose, Third Day: Vespers. “But how does it happen,” I said with admiration, “that you were able to solve the mystery of the library looking at it from the outside, and you were unable to solve it when you were inside?” “Thus, God knows the world, because He conceived it in His mind, as if it was from the outside, before it was created, and we do not know its rule, because we live inside it, having found it already made.",
journal = "Neuroscience Insights",
title = "Alzheimer’s and Consciousness: How Much Subjectivity Is Objective?",
volume = "16",
doi = "10.1177/26331055211033869"
}

Bajić, V. P., Misić, N., Stanković, I., Zarić, B.,& Perry, G.. (2021). Alzheimer’s and Consciousness: How Much Subjectivity Is Objective?. in Neuroscience Insights, 16.
https://doi.org/10.1177/26331055211033869

Bajić VP, Misić N, Stanković I, Zarić B, Perry G. Alzheimer’s and Consciousness: How Much Subjectivity Is Objective?. in Neuroscience Insights. 2021;16.
doi:10.1177/26331055211033869 .

Bajić, Vladan P., Misić, Nataša, Stanković, Ivana, Zarić, Božidarka, Perry, George, "Alzheimer’s and Consciousness: How Much Subjectivity Is Objective?" in Neuroscience Insights, 16 (2021),
https://doi.org/10.1177/26331055211033869 . .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Essack, Magbubah
AU  - Živković, Lada
AU  - Stewart, Alan J.
AU  - Zafirović, Sonja
AU  - Bajić, Vladimir B.
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8825
AB  - Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics. © Copyright © 2020 Bajic, Essack, Zivkovic, Stewart, Zafirovic, Bajic, Gojobori, Isenovic and Spremo-Potparevic.
T2  - Frontiers in Genetics
T1  - The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”
VL  - 10
DO  - 10.3389/fgene.2019.01368
ER  - 

@article{
author = "Bajić, Vladan P. and Essack, Magbubah and Živković, Lada and Stewart, Alan J. and Zafirović, Sonja and Bajić, Vladimir B. and Gojobori, Takashi and Isenović, Esma R. and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics. © Copyright © 2020 Bajic, Essack, Zivkovic, Stewart, Zafirovic, Bajic, Gojobori, Isenovic and Spremo-Potparevic.",
journal = "Frontiers in Genetics",
title = "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”",
volume = "10",
doi = "10.3389/fgene.2019.01368"
}

Bajić, V. P., Essack, M., Živković, L., Stewart, A. J., Zafirović, S., Bajić, V. B., Gojobori, T., Isenović, E. R.,& Spremo-Potparević, B.. (2020). The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. in Frontiers in Genetics, 10.
https://doi.org/10.3389/fgene.2019.01368

Bajić VP, Essack M, Živković L, Stewart AJ, Zafirović S, Bajić VB, Gojobori T, Isenović ER, Spremo-Potparević B. The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. in Frontiers in Genetics. 2020;10.
doi:10.3389/fgene.2019.01368 .

Bajić, Vladan P., Essack, Magbubah, Živković, Lada, Stewart, Alan J., Zafirović, Sonja, Bajić, Vladimir B., Gojobori, Takashi, Isenović, Esma R., Spremo-Potparević, Biljana, "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”" in Frontiers in Genetics, 10 (2020),
https://doi.org/10.3389/fgene.2019.01368 . .

TY  - JOUR
AU  - Dugalić, Predrag
AU  - Đuranović, Srđan
AU  - Pavlović-Marković, Aleksandra
AU  - Dugalić, Vladimir
AU  - Tomašević, Ratko
AU  - Gluvić, Zoran
AU  - Obradović, Milan M.
AU  - Bajić, Vladan P.
AU  - Isenović, Esma R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9104
AB  - Gastroesophageal Reflux Disease (GERD) is characterized by acid and bile reflux in the dis-tal oesophagus, and this may cause the development of reflux esophagitis and Barrett’s oesophagus (BE). The natural histological course of untreated BE is non-dysplastic or benign BE (ND), then low-grade (LGD) and High-Grade Dysplastic (HGD) BE, with the expected increase in malignancy transfer to oesophagal adenocarcinoma (EAC). The gold standard for BE diagnostics involves high-resolution white-light endoscopy, followed by uniform endoscopy findings description (Prague classification) with biopsy performance according to Seattle protocol. The medical treatment of GERD and BE includes the use of proton pump inhibitors (PPIs) regarding symptoms control. It is noteworthy that long-term use of PPIs increases gastrin level, which can contribute to transfer from BE to EAC, as a result of its effects on the proliferation of BE epithelium. Endoscopy treatment includes a wide range of re-section and ablative techniques, such as radio-frequency ablation (RFA), often concomitantly used in everyday endoscopy practice (multimodal therapy). RFA promotes mucosal necrosis of treated oesophagal region via high-frequency energy. Laparoscopic surgery, partial or total fundoplication, is reserved for PPIs and endoscopy indolent patients or in those with progressive disease. This review aims to explain distinct effects of PPIs and RFA modalities, illuminate certain aspects of molecular mechanisms involved, as well as the effects of their concomitant use regarding the treatment of BE and prevention of its transfer to EAC.
T2  - Mini-Reviews in Medicinal Chemistry
T1  - Proton Pump Inhibitors and Radiofrequency Ablation for Treatment of Barrett's Esophagus
VL  - 20
IS  - 11
SP  - 975
EP  - 987
DO  - 10.2174/1389557519666191015203636
ER  - 

@article{
author = "Dugalić, Predrag and Đuranović, Srđan and Pavlović-Marković, Aleksandra and Dugalić, Vladimir and Tomašević, Ratko and Gluvić, Zoran and Obradović, Milan M. and Bajić, Vladan P. and Isenović, Esma R.",
year = "2020",
abstract = "Gastroesophageal Reflux Disease (GERD) is characterized by acid and bile reflux in the dis-tal oesophagus, and this may cause the development of reflux esophagitis and Barrett’s oesophagus (BE). The natural histological course of untreated BE is non-dysplastic or benign BE (ND), then low-grade (LGD) and High-Grade Dysplastic (HGD) BE, with the expected increase in malignancy transfer to oesophagal adenocarcinoma (EAC). The gold standard for BE diagnostics involves high-resolution white-light endoscopy, followed by uniform endoscopy findings description (Prague classification) with biopsy performance according to Seattle protocol. The medical treatment of GERD and BE includes the use of proton pump inhibitors (PPIs) regarding symptoms control. It is noteworthy that long-term use of PPIs increases gastrin level, which can contribute to transfer from BE to EAC, as a result of its effects on the proliferation of BE epithelium. Endoscopy treatment includes a wide range of re-section and ablative techniques, such as radio-frequency ablation (RFA), often concomitantly used in everyday endoscopy practice (multimodal therapy). RFA promotes mucosal necrosis of treated oesophagal region via high-frequency energy. Laparoscopic surgery, partial or total fundoplication, is reserved for PPIs and endoscopy indolent patients or in those with progressive disease. This review aims to explain distinct effects of PPIs and RFA modalities, illuminate certain aspects of molecular mechanisms involved, as well as the effects of their concomitant use regarding the treatment of BE and prevention of its transfer to EAC.",
journal = "Mini-Reviews in Medicinal Chemistry",
title = "Proton Pump Inhibitors and Radiofrequency Ablation for Treatment of Barrett's Esophagus",
volume = "20",
number = "11",
pages = "975-987",
doi = "10.2174/1389557519666191015203636"
}

Dugalić, P., Đuranović, S., Pavlović-Marković, A., Dugalić, V., Tomašević, R., Gluvić, Z., Obradović, M. M., Bajić, V. P.,& Isenović, E. R.. (2020). Proton Pump Inhibitors and Radiofrequency Ablation for Treatment of Barrett's Esophagus. in Mini-Reviews in Medicinal Chemistry, 20(11), 975-987.
https://doi.org/10.2174/1389557519666191015203636

Dugalić P, Đuranović S, Pavlović-Marković A, Dugalić V, Tomašević R, Gluvić Z, Obradović MM, Bajić VP, Isenović ER. Proton Pump Inhibitors and Radiofrequency Ablation for Treatment of Barrett's Esophagus. in Mini-Reviews in Medicinal Chemistry. 2020;20(11):975-987.
doi:10.2174/1389557519666191015203636 .

Dugalić, Predrag, Đuranović, Srđan, Pavlović-Marković, Aleksandra, Dugalić, Vladimir, Tomašević, Ratko, Gluvić, Zoran, Obradović, Milan M., Bajić, Vladan P., Isenović, Esma R., "Proton Pump Inhibitors and Radiofrequency Ablation for Treatment of Barrett's Esophagus" in Mini-Reviews in Medicinal Chemistry, 20, no. 11 (2020):975-987,
https://doi.org/10.2174/1389557519666191015203636 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Essack, Magbubah
AU  - Zafirović, Sonja
AU  - Sudar-Milovanović, Emina
AU  - Bajić, Vladan P.
AU  - Van Neste, Christophe
AU  - Trpković, Andreja
AU  - Stanimirović, Julijana
AU  - Bajić, Vladimir B.
AU  - Isenović, Esma R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8486
AB  - Redox control is lost when the antioxidant defense system cannot remove abnormally high concentrations of signaling molecules, such as reactive oxygen species (ROS). Chronically elevated levels of ROS cause oxidative stress that may eventually lead to cancer and cardiovascular and neurodegenerative diseases. In this review, we focus on redox effects in the vascular system. We pay close attention to the subcompartments of the vascular system (endothelium, smooth muscle cell layer) and give an overview of how redox changes influence those different compartments. We also review the core aspects of redox biology, cardiovascular physiology, and pathophysiology. Moreover, the topic-specific knowledgebase DES-RedoxVasc was used to develop two case studies, one focused on endothelial cells and the other on the vascular smooth muscle cells, as a starting point to possibly extend our knowledge of redox control in vascular biology. © 2019 The Authors. BioFactors published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology.
T2  - BioFactors
T1  - Redox control of vascular biology
VL  - 46
IS  - 2
SP  - 246
EP  - 262
DO  - 10.1002/biof.1559
ER  - 

@article{
author = "Obradović, Milan M. and Essack, Magbubah and Zafirović, Sonja and Sudar-Milovanović, Emina and Bajić, Vladan P. and Van Neste, Christophe and Trpković, Andreja and Stanimirović, Julijana and Bajić, Vladimir B. and Isenović, Esma R.",
year = "2020",
abstract = "Redox control is lost when the antioxidant defense system cannot remove abnormally high concentrations of signaling molecules, such as reactive oxygen species (ROS). Chronically elevated levels of ROS cause oxidative stress that may eventually lead to cancer and cardiovascular and neurodegenerative diseases. In this review, we focus on redox effects in the vascular system. We pay close attention to the subcompartments of the vascular system (endothelium, smooth muscle cell layer) and give an overview of how redox changes influence those different compartments. We also review the core aspects of redox biology, cardiovascular physiology, and pathophysiology. Moreover, the topic-specific knowledgebase DES-RedoxVasc was used to develop two case studies, one focused on endothelial cells and the other on the vascular smooth muscle cells, as a starting point to possibly extend our knowledge of redox control in vascular biology. © 2019 The Authors. BioFactors published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology.",
journal = "BioFactors",
title = "Redox control of vascular biology",
volume = "46",
number = "2",
pages = "246-262",
doi = "10.1002/biof.1559"
}

Obradović, M. M., Essack, M., Zafirović, S., Sudar-Milovanović, E., Bajić, V. P., Van Neste, C., Trpković, A., Stanimirović, J., Bajić, V. B.,& Isenović, E. R.. (2020). Redox control of vascular biology. in BioFactors, 46(2), 246-262.
https://doi.org/10.1002/biof.1559

Obradović MM, Essack M, Zafirović S, Sudar-Milovanović E, Bajić VP, Van Neste C, Trpković A, Stanimirović J, Bajić VB, Isenović ER. Redox control of vascular biology. in BioFactors. 2020;46(2):246-262.
doi:10.1002/biof.1559 .

Obradović, Milan M., Essack, Magbubah, Zafirović, Sonja, Sudar-Milovanović, Emina, Bajić, Vladan P., Van Neste, Christophe, Trpković, Andreja, Stanimirović, Julijana, Bajić, Vladimir B., Isenović, Esma R., "Redox control of vascular biology" in BioFactors, 46, no. 2 (2020):246-262,
https://doi.org/10.1002/biof.1559 . .

TY  - JOUR
AU  - Essack, Magbubah
AU  - Salhi, Adil
AU  - Van Neste, Christophe
AU  - Raies, Arwa Bin
AU  - Tifratene, Faroug
AU  - Uludag, Mahmut
AU  - Hungler, Arnaud
AU  - Zarić, Božidarka
AU  - Zafirović, Sonja
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Bajić, Vladan P.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8945
AB  - Normal cellular physiology and biochemical processes require undamaged RNA molecules. However, RNAs are frequently subjected to oxidative damage. Overproduction of reactive oxygen species (ROS) leads to RNA oxidation and disturbs redox (oxidation-reduction reaction) homeostasis. When oxidation damage affects RNA carrying protein-coding information, this may result in the synthesis of aberrant proteins as well as a lower efficiency of translation. Both of these, as well as imbalanced redox homeostasis, may lead to numerous human diseases. The number of studies on the effects of RNA oxidative damage in mammals is increasing by year due to the understanding that this oxidation fundamentally leads to numerous human diseases. To enable researchers in this field to explore information relevant to RNA oxidation and effects on human diseases, we developed DES-ROD, an online knowledgebase that contains processed information from 298,603 relevant documents that consist of PubMed abstracts and PubMed Central full-text articles. The system utilizes concepts/terms from 38 curated thematic dictionaries mapped to the analyzed documents. Researchers can explore enriched concepts, as well as enriched pairs of putatively associated concepts. In this way, one can explore mutual relationships between any combinations of two concepts from used dictionaries. Dictionaries cover a wide range of biomedical topics, such as human genes and proteins, pathways, Gene Ontology categories, mutations, noncoding RNAs, enzymes, toxins, metabolites, and diseases. This makes insights into different facets of the effects of RNA oxidation and the control of this process possible. The usefulness of the DES-ROD system is demonstrated by case studies on some known information, as well as potentially novel information involving RNA oxidation and diseases. DES-ROD is the first knowledgebase based on text and data mining that focused on the exploration of RNA oxidation and human diseases.
T2  - Oxidative Medicine and Cellular Longevity
T1  - DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases
VL  - 2020
SP  - 5904315
DO  - 10.1155/2020/5904315
ER  - 

@article{
author = "Essack, Magbubah and Salhi, Adil and Van Neste, Christophe and Raies, Arwa Bin and Tifratene, Faroug and Uludag, Mahmut and Hungler, Arnaud and Zarić, Božidarka and Zafirović, Sonja and Gojobori, Takashi and Isenović, Esma R. and Bajić, Vladan P.",
year = "2020",
abstract = "Normal cellular physiology and biochemical processes require undamaged RNA molecules. However, RNAs are frequently subjected to oxidative damage. Overproduction of reactive oxygen species (ROS) leads to RNA oxidation and disturbs redox (oxidation-reduction reaction) homeostasis. When oxidation damage affects RNA carrying protein-coding information, this may result in the synthesis of aberrant proteins as well as a lower efficiency of translation. Both of these, as well as imbalanced redox homeostasis, may lead to numerous human diseases. The number of studies on the effects of RNA oxidative damage in mammals is increasing by year due to the understanding that this oxidation fundamentally leads to numerous human diseases. To enable researchers in this field to explore information relevant to RNA oxidation and effects on human diseases, we developed DES-ROD, an online knowledgebase that contains processed information from 298,603 relevant documents that consist of PubMed abstracts and PubMed Central full-text articles. The system utilizes concepts/terms from 38 curated thematic dictionaries mapped to the analyzed documents. Researchers can explore enriched concepts, as well as enriched pairs of putatively associated concepts. In this way, one can explore mutual relationships between any combinations of two concepts from used dictionaries. Dictionaries cover a wide range of biomedical topics, such as human genes and proteins, pathways, Gene Ontology categories, mutations, noncoding RNAs, enzymes, toxins, metabolites, and diseases. This makes insights into different facets of the effects of RNA oxidation and the control of this process possible. The usefulness of the DES-ROD system is demonstrated by case studies on some known information, as well as potentially novel information involving RNA oxidation and diseases. DES-ROD is the first knowledgebase based on text and data mining that focused on the exploration of RNA oxidation and human diseases.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases",
volume = "2020",
pages = "5904315",
doi = "10.1155/2020/5904315"
}

Essack, M., Salhi, A., Van Neste, C., Raies, A. B., Tifratene, F., Uludag, M., Hungler, A., Zarić, B., Zafirović, S., Gojobori, T., Isenović, E. R.,& Bajić, V. P.. (2020). DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases. in Oxidative Medicine and Cellular Longevity, 2020, 5904315.
https://doi.org/10.1155/2020/5904315

Essack M, Salhi A, Van Neste C, Raies AB, Tifratene F, Uludag M, Hungler A, Zarić B, Zafirović S, Gojobori T, Isenović ER, Bajić VP. DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases. in Oxidative Medicine and Cellular Longevity. 2020;2020:5904315.
doi:10.1155/2020/5904315 .

Essack, Magbubah, Salhi, Adil, Van Neste, Christophe, Raies, Arwa Bin, Tifratene, Faroug, Uludag, Mahmut, Hungler, Arnaud, Zarić, Božidarka, Zafirović, Sonja, Gojobori, Takashi, Isenović, Esma R., Bajić, Vladan P., "DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases" in Oxidative Medicine and Cellular Longevity, 2020 (2020):5904315,
https://doi.org/10.1155/2020/5904315 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9810
AB  - Objective. Inosine 5'-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions.
AB  - Cilj. Aktivnost inozin 5’-monofosfat dehidrogenaze(IMPDH) povećana je u ćelijama karcinoma. Tiazofurin selektivno inhibira aktivnost IMPDH i odobren je za lečenje različitih karcinoma i novih virusnih bolesti. Njegova široko rasprostranjena upotreba bila je ograničena jer je utvrđenoda je izloženost tiazofurinu pod određenim okolnostima imala veću incidencu ozbiljne nehematološke toksičnosti. Stoga je cilj ove studije bio da se pomoću komet testa ispita genotoksično delovanje i izazivanje DNK oštećenja tiazofurinom. Metode. Sposobnost tiazofurina da izazove DNK oštećenje procenjena je primenom elektroforeze DNK pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su bile izložene trima konačnim koncentracijama tiazofurina (1µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro. Rezultati. Naši rezultati ukazuju na to da je tiazofurin proizveo značajan nivo DNK oštećenja na ćelijama pune krvi nakon 30 minuta izlaganja u odnosu na kontrolu. Sve ispitivane koncentracije su dovele do značajnog nastanka kometa, pri čemu je nivo oštećenja rastao s koncentracijom. Zaključak. Naše istraživanje ćelija tretiranih tiazofurinom i njihova reakcija na izazivanje DNK oštećenja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima.
T2  - Medicinski časopis
T1  - Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay
T1  - Analiza dnk oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa
VL  - 54
IS  - 3
SP  - 91
EP  - 95
DO  - 10.5937/mckg54-28798
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Đelić, Ninoslav and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Objective. Inosine 5'-monophosphate dehydrogenase (IMPDH) activity in cancer cells is increased. Tiazofurin selectively inhibits the activity of IMPDH, and it has been granted for the treatment of different cancers and new viral diseases. Its widespread use was limited because exposure to tiazofurin under certain circumstances was found to have a higher frequency of severe non-hematologic toxicity. Therefore, the objective of this study was to examine genotoxic action and inducement of DNA damage of tiazofurin using the comet assay. Methods. The ability of tiazofurin to induce DNA damage was evaluated using single-cell gel electrophoresis (SCGE) technique/comet assay. Human whole blood cells were exposed to three final concentrations of tiazofurin (1µM/mL, 2 µM/mL, and 5 µM/mL) for 30 min in vitro. Results. Our results indicate that tiazofurin produced a significant level of DNA damage on whole blood cells after 30 min of exposure vs. control. All tested concentrations were significantly comet-forming, in a concentration-dependent manner. Conclusion. Our investigation on the tiazofurin-treated cells and their relationship to the formation of DNA damage demonstrated that the genotoxic effect was induced after exposure to tiazofurin under described conditions., Cilj. Aktivnost inozin 5’-monofosfat dehidrogenaze(IMPDH) povećana je u ćelijama karcinoma. Tiazofurin selektivno inhibira aktivnost IMPDH i odobren je za lečenje različitih karcinoma i novih virusnih bolesti. Njegova široko rasprostranjena upotreba bila je ograničena jer je utvrđenoda je izloženost tiazofurinu pod određenim okolnostima imala veću incidencu ozbiljne nehematološke toksičnosti. Stoga je cilj ove studije bio da se pomoću komet testa ispita genotoksično delovanje i izazivanje DNK oštećenja tiazofurinom. Metode. Sposobnost tiazofurina da izazove DNK oštećenje procenjena je primenom elektroforeze DNK pojedinačnih ćelija (SCGE) / komet testa. Ćelije pune krvi su bile izložene trima konačnim koncentracijama tiazofurina (1µM/mL, 2 µM/mL, and 5 µM/mL) tokom 30 minuta in vitro. Rezultati. Naši rezultati ukazuju na to da je tiazofurin proizveo značajan nivo DNK oštećenja na ćelijama pune krvi nakon 30 minuta izlaganja u odnosu na kontrolu. Sve ispitivane koncentracije su dovele do značajnog nastanka kometa, pri čemu je nivo oštećenja rastao s koncentracijom. Zaključak. Naše istraživanje ćelija tretiranih tiazofurinom i njihova reakcija na izazivanje DNK oštećenja pokazalo je da je tiazofurin ispoljio genotoksični efekat pod opisanim uslovima.",
journal = "Medicinski časopis",
title = "Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay, Analiza dnk oštećenja izazvanog tiazofurinom u humanim ćelijama pune krvi primenom in vitro komet testa",
volume = "54",
number = "3",
pages = "91-95",
doi = "10.5937/mckg54-28798"
}

Topalović, D., Živković, L., Đelić, N., Bajić, V. P.,& Spremo-Potparević, B.. (2020). Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay. in Medicinski časopis, 54(3), 91-95.
https://doi.org/10.5937/mckg54-28798

Topalović D, Živković L, Đelić N, Bajić VP, Spremo-Potparević B. Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay. in Medicinski časopis. 2020;54(3):91-95.
doi:10.5937/mckg54-28798 .

Topalović, Dijana, Živković, Lada, Đelić, Ninoslav, Bajić, Vladan P., Spremo-Potparević, Biljana, "Analysis of tiazofurin-induced DNA damage in human whole blood cells using an in vitro comet assay" in Medicinski časopis, 54, no. 3 (2020):91-95,
https://doi.org/10.5937/mckg54-28798 . .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Van Neste, Christophe
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Radak, Đorđe J.
AU  - Bajić, Vladimir B.
AU  - Essack, Magbubah
AU  - Isenović, Esma R.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8375
AB  - More people die from cardiovascular diseases (CVD) than from any other cause. Cardiovascular complications are thought to arise from enhanced levels of free radicals causing impaired “redox homeostasis,” which represents the interplay between oxidative stress (OS) and reductive stress (RS). In this review, we compile several experimental research findings that show sustained shifts towards OS will alter the homeostatic redox mechanism to cause cardiovascular complications, as well as findings that show a prolonged antioxidant state or RS can similarly lead to such cardiovascular complications. This experimental evidence is specifically focused on the role of glutathione, the most abundant antioxidant in the heart, in a redox homeostatic mechanism that has been shifted towards OS or RS. This may lead to impairment of cellular signaling mechanisms and elevated pools of proteotoxicity associated with cardiac dysfunction.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease
VL  - 2019
SP  - 5028181
DO  - 10.1155/2019/5028181
ER  - 

@article{
author = "Bajić, Vladan P. and Van Neste, Christophe and Obradović, Milan M. and Zafirović, Sonja and Radak, Đorđe J. and Bajić, Vladimir B. and Essack, Magbubah and Isenović, Esma R.",
year = "2019",
abstract = "More people die from cardiovascular diseases (CVD) than from any other cause. Cardiovascular complications are thought to arise from enhanced levels of free radicals causing impaired “redox homeostasis,” which represents the interplay between oxidative stress (OS) and reductive stress (RS). In this review, we compile several experimental research findings that show sustained shifts towards OS will alter the homeostatic redox mechanism to cause cardiovascular complications, as well as findings that show a prolonged antioxidant state or RS can similarly lead to such cardiovascular complications. This experimental evidence is specifically focused on the role of glutathione, the most abundant antioxidant in the heart, in a redox homeostatic mechanism that has been shifted towards OS or RS. This may lead to impairment of cellular signaling mechanisms and elevated pools of proteotoxicity associated with cardiac dysfunction.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease",
volume = "2019",
pages = "5028181",
doi = "10.1155/2019/5028181"
}

Bajić, V. P., Van Neste, C., Obradović, M. M., Zafirović, S., Radak, Đ. J., Bajić, V. B., Essack, M.,& Isenović, E. R.. (2019). Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease. in Oxidative Medicine and Cellular Longevity, 2019, 5028181.
https://doi.org/10.1155/2019/5028181

Bajić VP, Van Neste C, Obradović MM, Zafirović S, Radak ĐJ, Bajić VB, Essack M, Isenović ER. Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease. in Oxidative Medicine and Cellular Longevity. 2019;2019:5028181.
doi:10.1155/2019/5028181 .

Bajić, Vladan P., Van Neste, Christophe, Obradović, Milan M., Zafirović, Sonja, Radak, Đorđe J., Bajić, Vladimir B., Essack, Magbubah, Isenović, Esma R., "Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease" in Oxidative Medicine and Cellular Longevity, 2019 (2019):5028181,
https://doi.org/10.1155/2019/5028181 . .

TY  - JOUR
AU  - Zarić, Božidarka
AU  - Obradović, Milan M.
AU  - Bajić, Vladan P.
AU  - Haidara, Mohamed A.
AU  - Jovanović, Miloš
AU  - Isenović, Esma R.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8485
AB  - Homocysteine (Hcy) is a thiol group containing the amino acid, which naturally occurs in all humans. Hcy is degraded in the body through two metabolic pathways, while a minor part is excreted through kidneys. The chemical reactions that are necessary for degradation of Hcy require the presence of folic acid, vitamins B6 and B12. Consequently, the level of the total Hcy in the serum is influenced by the presence or absence of these vitamins. An elevated level of the Hcy, hyperhomocysteinemia (HHcy) and homocystinuria is connected with occlusive artery disease, especially in the brain, the heart, and the kidney, in addition to venous thrombosis, chronic renal failure, megaloblastic anemia, osteoporosis, depression, Alzheimer's disease, pregnancy problems, and others. Elevated Hcy levels are connected with various pathologies both in adult and child population. Causes of HHcy include genetic mutations and enzyme deficiencies in 5, 10-methylenetetrahydrofolate reductase (MTHFR) methionine synthase (MS), and cystathionine β-synthase (CβS). HHcy can be caused by deficiencies in the folate, vitamin B12 and to a lesser extent, deficiency in B6 vitamin what influences methionine metabolism. Additionally, HHcy can be caused by the rich diet and renal impairment. This review presents literature data from recent research related to Hcy metabolism and the etiology of the Hcy blood level disorder. In addition, we also described various pathological mechanisms induced by hereditary disturbances or nutritional influences and their association with HHcy induced pathology in adults and children and treatment of these metabolic disorders. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
T2  - Current Medicinal Chemistry
T1  - Homocysteine and Hyperhomocysteinaemia
VL  - 26
IS  - 16
SP  - 2948
EP  - 2961
DO  - 10.2174/0929867325666180313105949
ER  - 

@article{
author = "Zarić, Božidarka and Obradović, Milan M. and Bajić, Vladan P. and Haidara, Mohamed A. and Jovanović, Miloš and Isenović, Esma R.",
year = "2019",
abstract = "Homocysteine (Hcy) is a thiol group containing the amino acid, which naturally occurs in all humans. Hcy is degraded in the body through two metabolic pathways, while a minor part is excreted through kidneys. The chemical reactions that are necessary for degradation of Hcy require the presence of folic acid, vitamins B6 and B12. Consequently, the level of the total Hcy in the serum is influenced by the presence or absence of these vitamins. An elevated level of the Hcy, hyperhomocysteinemia (HHcy) and homocystinuria is connected with occlusive artery disease, especially in the brain, the heart, and the kidney, in addition to venous thrombosis, chronic renal failure, megaloblastic anemia, osteoporosis, depression, Alzheimer's disease, pregnancy problems, and others. Elevated Hcy levels are connected with various pathologies both in adult and child population. Causes of HHcy include genetic mutations and enzyme deficiencies in 5, 10-methylenetetrahydrofolate reductase (MTHFR) methionine synthase (MS), and cystathionine β-synthase (CβS). HHcy can be caused by deficiencies in the folate, vitamin B12 and to a lesser extent, deficiency in B6 vitamin what influences methionine metabolism. Additionally, HHcy can be caused by the rich diet and renal impairment. This review presents literature data from recent research related to Hcy metabolism and the etiology of the Hcy blood level disorder. In addition, we also described various pathological mechanisms induced by hereditary disturbances or nutritional influences and their association with HHcy induced pathology in adults and children and treatment of these metabolic disorders. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.",
journal = "Current Medicinal Chemistry",
title = "Homocysteine and Hyperhomocysteinaemia",
volume = "26",
number = "16",
pages = "2948-2961",
doi = "10.2174/0929867325666180313105949"
}

Zarić, B., Obradović, M. M., Bajić, V. P., Haidara, M. A., Jovanović, M.,& Isenović, E. R.. (2019). Homocysteine and Hyperhomocysteinaemia. in Current Medicinal Chemistry, 26(16), 2948-2961.
https://doi.org/10.2174/0929867325666180313105949

Zarić B, Obradović MM, Bajić VP, Haidara MA, Jovanović M, Isenović ER. Homocysteine and Hyperhomocysteinaemia. in Current Medicinal Chemistry. 2019;26(16):2948-2961.
doi:10.2174/0929867325666180313105949 .

Zarić, Božidarka, Obradović, Milan M., Bajić, Vladan P., Haidara, Mohamed A., Jovanović, Miloš, Isenović, Esma R., "Homocysteine and Hyperhomocysteinaemia" in Current Medicinal Chemistry, 26, no. 16 (2019):2948-2961,
https://doi.org/10.2174/0929867325666180313105949 . .

TY  - JOUR
AU  - Essack, Magbubah
AU  - Salhi, Adil
AU  - Stanimirović, Julijana
AU  - Tifratene, Faroug
AU  - Bin Raies, Arwa
AU  - Hungler, Arnaud
AU  - Uludag, Mahmut
AU  - Van Neste, Christophe
AU  - Trpković, Andreja
AU  - Bajić, Vladan P.
AU  - Bajić, Vladimir B.
AU  - Isenović, Esma R.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8389
AB  - In cellular physiology and signaling, reactive oxygen species (ROS) play one of the most critical roles. ROS overproduction leads to cellular oxidative stress. This may lead to an irrecoverable imbalance of redox (oxidation-reduction reaction) function that deregulates redox homeostasis, which itself could lead to several diseases including neurodegenerative disease, cardiovascular disease, and cancers. In this study, we focus on the redox effects related to vascular systems in mammals. To support research in this domain, we developed an online knowledge base, DES-RedoxVasc, which enables exploration of information contained in the biomedical scientific literature. The DES-RedoxVasc system analyzed 233399 documents consisting of PubMed abstracts and PubMed Central full-text articles related to different aspects of redox biology in vascular systems. It allows researchers to explore enriched concepts from 28 curated thematic dictionaries, as well as literature-derived potential associations of pairs of such enriched concepts, where associations themselves are statistically enriched. For example, the system allows exploration of associations of pathways, diseases, mutations, genes/proteins, miRNAs, long ncRNAs, toxins, drugs, biological processes, molecular functions, etc. that allow for insights about different aspects of redox effects and control of processes related to the vascular system. Moreover, we deliver case studies about some existing or possibly novel knowledge regarding redox of vascular biology demonstrating the usefulness of DES-RedoxVasc. DES-RedoxVasc is the first compiled knowledge base using text mining for the exploration of this topic.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Literature-Based Enrichment Insights into Redox Control of Vascular Biology
VL  - 2019
SP  - 1769437
DO  - 10.1155/2019/1769437
ER  - 

@article{
author = "Essack, Magbubah and Salhi, Adil and Stanimirović, Julijana and Tifratene, Faroug and Bin Raies, Arwa and Hungler, Arnaud and Uludag, Mahmut and Van Neste, Christophe and Trpković, Andreja and Bajić, Vladan P. and Bajić, Vladimir B. and Isenović, Esma R.",
year = "2019",
abstract = "In cellular physiology and signaling, reactive oxygen species (ROS) play one of the most critical roles. ROS overproduction leads to cellular oxidative stress. This may lead to an irrecoverable imbalance of redox (oxidation-reduction reaction) function that deregulates redox homeostasis, which itself could lead to several diseases including neurodegenerative disease, cardiovascular disease, and cancers. In this study, we focus on the redox effects related to vascular systems in mammals. To support research in this domain, we developed an online knowledge base, DES-RedoxVasc, which enables exploration of information contained in the biomedical scientific literature. The DES-RedoxVasc system analyzed 233399 documents consisting of PubMed abstracts and PubMed Central full-text articles related to different aspects of redox biology in vascular systems. It allows researchers to explore enriched concepts from 28 curated thematic dictionaries, as well as literature-derived potential associations of pairs of such enriched concepts, where associations themselves are statistically enriched. For example, the system allows exploration of associations of pathways, diseases, mutations, genes/proteins, miRNAs, long ncRNAs, toxins, drugs, biological processes, molecular functions, etc. that allow for insights about different aspects of redox effects and control of processes related to the vascular system. Moreover, we deliver case studies about some existing or possibly novel knowledge regarding redox of vascular biology demonstrating the usefulness of DES-RedoxVasc. DES-RedoxVasc is the first compiled knowledge base using text mining for the exploration of this topic.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Literature-Based Enrichment Insights into Redox Control of Vascular Biology",
volume = "2019",
pages = "1769437",
doi = "10.1155/2019/1769437"
}

Essack, M., Salhi, A., Stanimirović, J., Tifratene, F., Bin Raies, A., Hungler, A., Uludag, M., Van Neste, C., Trpković, A., Bajić, V. P., Bajić, V. B.,& Isenović, E. R.. (2019). Literature-Based Enrichment Insights into Redox Control of Vascular Biology. in Oxidative Medicine and Cellular Longevity, 2019, 1769437.
https://doi.org/10.1155/2019/1769437

Essack M, Salhi A, Stanimirović J, Tifratene F, Bin Raies A, Hungler A, Uludag M, Van Neste C, Trpković A, Bajić VP, Bajić VB, Isenović ER. Literature-Based Enrichment Insights into Redox Control of Vascular Biology. in Oxidative Medicine and Cellular Longevity. 2019;2019:1769437.
doi:10.1155/2019/1769437 .

Essack, Magbubah, Salhi, Adil, Stanimirović, Julijana, Tifratene, Faroug, Bin Raies, Arwa, Hungler, Arnaud, Uludag, Mahmut, Van Neste, Christophe, Trpković, Andreja, Bajić, Vladan P., Bajić, Vladimir B., Isenović, Esma R., "Literature-Based Enrichment Insights into Redox Control of Vascular Biology" in Oxidative Medicine and Cellular Longevity, 2019 (2019):1769437,
https://doi.org/10.1155/2019/1769437 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan P.
AU  - Topalović, Dijana
AU  - Bruić, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8666
AB  - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μ g/mL, 500 μ g/mL and 1000 μ g/mL) and dihydroquercetin (100 μ g/mL, 250 μ g/mL and 500 μ g/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H 2 O 2 -) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H 2 O 2 -induced damage on DNA in cells at all tested concentrations, with a statistical significance ( p < 0.05 ), whereas Biochaga at the dose of 500 μ g/mL in combination with dihydroquercetin 500 μ g/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro .
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells
VL  - 2019
SP  - 5039372
DO  - 10.1155/2019/5039372
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan P. and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana",
year = "2019",
abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μ g/mL, 500 μ g/mL and 1000 μ g/mL) and dihydroquercetin (100 μ g/mL, 250 μ g/mL and 500 μ g/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H 2 O 2 -) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H 2 O 2 -induced damage on DNA in cells at all tested concentrations, with a statistical significance ( p < 0.05 ), whereas Biochaga at the dose of 500 μ g/mL in combination with dihydroquercetin 500 μ g/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro .",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells",
volume = "2019",
pages = "5039372",
doi = "10.1155/2019/5039372"
}

Živković, L., Bajić, V. P., Topalović, D., Bruić, M.,& Spremo-Potparević, B.. (2019). Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. in Oxidative Medicine and Cellular Longevity, 2019, 5039372.
https://doi.org/10.1155/2019/5039372

Živković L, Bajić VP, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. in Oxidative Medicine and Cellular Longevity. 2019;2019:5039372.
doi:10.1155/2019/5039372 .

Živković, Lada, Bajić, Vladan P., Topalović, Dijana, Bruić, Marija, Spremo-Potparević, Biljana, "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells" in Oxidative Medicine and Cellular Longevity, 2019 (2019):5039372,
https://doi.org/10.1155/2019/5039372 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Bajić, Vladan P.
AU  - Đorđević, Stefana
AU  - Hristov, Aleksandar
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9002
AB  - Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078-2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078-10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers.
AB  - Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) - skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.
T2  - Medicinski časopis
T1  - Evaluation of antioxidant potential of Cordyceps sinensis in vitro
T1  - Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro
VL  - 53
IS  - 4
SP  - 129
EP  - 134
DO  - 10.5937/mckg53-24450
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica Z. and Bajić, Vladan P. and Đorđević, Stefana and Hristov, Aleksandar and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078-2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078-10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers., Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) - skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.",
journal = "Medicinski časopis",
title = "Evaluation of antioxidant potential of Cordyceps sinensis in vitro, Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro",
volume = "53",
number = "4",
pages = "129-134",
doi = "10.5937/mckg53-24450"
}

Živković, L., Borozan, S. Z., Bajić, V. P., Đorđević, S., Hristov, A.,& Spremo-Potparević, B.. (2019). Evaluation of antioxidant potential of Cordyceps sinensis in vitro. in Medicinski časopis, 53(4), 129-134.
https://doi.org/10.5937/mckg53-24450

Živković L, Borozan SZ, Bajić VP, Đorđević S, Hristov A, Spremo-Potparević B. Evaluation of antioxidant potential of Cordyceps sinensis in vitro. in Medicinski časopis. 2019;53(4):129-134.
doi:10.5937/mckg53-24450 .

Živković, Lada, Borozan, Sunčica Z., Bajić, Vladan P., Đorđević, Stefana, Hristov, Aleksandar, Spremo-Potparević, Biljana, "Evaluation of antioxidant potential of Cordyceps sinensis in vitro" in Medicinski časopis, 53, no. 4 (2019):129-134,
https://doi.org/10.5937/mckg53-24450 . .

TY  - JOUR
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan P.
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Sredojević, Dušan
AU  - Lazić, Vesna M.
AU  - Nedeljković, Jovan
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0278691518301388
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7790
AB  - The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.
T2  - Food and Chemical Toxicology
T1  - Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid
VL  - 115
SP  - 42
EP  - 48
DO  - 10.1016/j.fct.2018.02.064
ER  - 

@article{
author = "Dekanski, Dragana and Spremo-Potparević, Biljana and Bajić, Vladan P. and Živković, Lada and Topalović, Dijana and Sredojević, Dušan and Lazić, Vesna M. and Nedeljković, Jovan",
year = "2018",
abstract = "The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.",
journal = "Food and Chemical Toxicology",
title = "Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid",
volume = "115",
pages = "42-48",
doi = "10.1016/j.fct.2018.02.064"
}

Dekanski, D., Spremo-Potparević, B., Bajić, V. P., Živković, L., Topalović, D., Sredojević, D., Lazić, V. M.,& Nedeljković, J.. (2018). Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid. in Food and Chemical Toxicology, 115, 42-48.
https://doi.org/10.1016/j.fct.2018.02.064

Dekanski D, Spremo-Potparević B, Bajić VP, Živković L, Topalović D, Sredojević D, Lazić VM, Nedeljković J. Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid. in Food and Chemical Toxicology. 2018;115:42-48.
doi:10.1016/j.fct.2018.02.064 .

Dekanski, Dragana, Spremo-Potparević, Biljana, Bajić, Vladan P., Živković, Lada, Topalović, Dijana, Sredojević, Dušan, Lazić, Vesna M., Nedeljković, Jovan, "Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid" in Food and Chemical Toxicology, 115 (2018):42-48,
https://doi.org/10.1016/j.fct.2018.02.064 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan P.
AU  - Dekanski, Dragana
AU  - Čabarkapa-Pirković, Andrea
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Mazzoni, Luca
AU  - Spremo-Potparević, Biljana
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S027869151830334X
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7819
AB  - Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.
T2  - Food and Chemical Toxicology
T1  - Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro
VL  - 119
SP  - 61
EP  - 65
DO  - 10.1016/j.fct.2018.05.034
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan P. and Dekanski, Dragana and Čabarkapa-Pirković, Andrea and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Spremo-Potparević, Biljana",
year = "2018",
abstract = "Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.",
journal = "Food and Chemical Toxicology",
title = "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro",
volume = "119",
pages = "61-65",
doi = "10.1016/j.fct.2018.05.034"
}

Živković, L., Bajić, V. P., Dekanski, D., Čabarkapa-Pirković, A., Giampieri, F., Gasparrini, M., Mazzoni, L.,& Spremo-Potparević, B.. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology, 119, 61-65.
https://doi.org/10.1016/j.fct.2018.05.034

Živković L, Bajić VP, Dekanski D, Čabarkapa-Pirković A, Giampieri F, Gasparrini M, Mazzoni L, Spremo-Potparević B. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology. 2018;119:61-65.
doi:10.1016/j.fct.2018.05.034 .

Živković, Lada, Bajić, Vladan P., Dekanski, Dragana, Čabarkapa-Pirković, Andrea, Giampieri, Francesca, Gasparrini, Massimiliano, Mazzoni, Luca, Spremo-Potparević, Biljana, "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro" in Food and Chemical Toxicology, 119 (2018):61-65,
https://doi.org/10.1016/j.fct.2018.05.034 . .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Kotur-Stevuljevic, Jelena
AU  - Isenović, Esma R.
AU  - Sredojević, Dušan
AU  - Vukoje, Ivana D.
AU  - Lazić, Vesna M.
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1607
AB  - The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Jobs method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro
VL  - 155
SP  - 323
EP  - 331
DO  - 10.1016/j.colsurfb.2017.04.032
ER  - 

@article{
author = "Bajić, Vladan P. and Spremo-Potparević, Biljana and Živković, Lada and Čabarkapa, Andrea and Kotur-Stevuljevic, Jelena and Isenović, Esma R. and Sredojević, Dušan and Vukoje, Ivana D. and Lazić, Vesna M. and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2017",
abstract = "The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Jobs method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro",
volume = "155",
pages = "323-331",
doi = "10.1016/j.colsurfb.2017.04.032"
}

Bajić, V. P., Spremo-Potparević, B., Živković, L., Čabarkapa, A., Kotur-Stevuljevic, J., Isenović, E. R., Sredojević, D., Vukoje, I. D., Lazić, V. M., Ahrenkiel, S. P.,& Nedeljković, J.. (2017). Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces. B: Biointerfaces, 155, 323-331.
https://doi.org/10.1016/j.colsurfb.2017.04.032

Bajić VP, Spremo-Potparević B, Živković L, Čabarkapa A, Kotur-Stevuljevic J, Isenović ER, Sredojević D, Vukoje ID, Lazić VM, Ahrenkiel SP, Nedeljković J. Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces. B: Biointerfaces. 2017;155:323-331.
doi:10.1016/j.colsurfb.2017.04.032 .

Bajić, Vladan P., Spremo-Potparević, Biljana, Živković, Lada, Čabarkapa, Andrea, Kotur-Stevuljevic, Jelena, Isenović, Esma R., Sredojević, Dušan, Vukoje, Ivana D., Lazić, Vesna M., Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro" in Colloids and Surfaces. B: Biointerfaces, 155 (2017):323-331,
https://doi.org/10.1016/j.colsurfb.2017.04.032 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Davidović, Radoslav S.
AU  - Bajić, Vladan P.
AU  - Obradović, Milan M.
AU  - Isenović, Esma R.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1614
AB  - Breast cancer (BC) is a heterogeneous disease in an urgent need for developing novel research, classification, and therapy approaches. Breast cancer 1 (BRCA1) and breast cancer 2 (BRCA2) proteins are well described tumor suppressors with great potential to be the subjects of different therapies. MicroRNAs (miRNAs) are genetic elements that might be used to solve the complex BC puzzle. BRCA1 was described to be the target of up to 100 miRNAs. BRCA1 may directly repress miR-155 activity. In addition, miR-15/107/182-mediated downregulation of BRCA1 interrupt DNA repair and may change the course of BC therapy. miR-146a and miR-146-5p silencing BRCA1 may trigger formation of triple-negative and basal-like sporadic BC cases. miR-182 might effect the therapy outcome. miR-21 targeted therapy might be useful for the treatment of BRCA2 mutation carriers. miR-342 overexpression and the absence of functional BRCA1 gene might cause synthetic lethality, which might be used as a base for future therapies. The present review discusses the latest data from studies that focus on the complex network of miRNAs and BRCA1/2 related BCs, which might be important for improving the therapy within the patients with triple-negative BC (TNBC) and basal-like BC, and for understanding the formation of TNBC.
T2  - Cancer Biomarkers
T1  - MicroRNA in breast cancer: The association with BRCA1/2
VL  - 19
IS  - 2
SP  - 119
EP  - 128
DO  - 10.3233/CBM-160319
ER  - 

@article{
author = "Petrović, Nina and Davidović, Radoslav S. and Bajić, Vladan P. and Obradović, Milan M. and Isenović, Esma R.",
year = "2017",
abstract = "Breast cancer (BC) is a heterogeneous disease in an urgent need for developing novel research, classification, and therapy approaches. Breast cancer 1 (BRCA1) and breast cancer 2 (BRCA2) proteins are well described tumor suppressors with great potential to be the subjects of different therapies. MicroRNAs (miRNAs) are genetic elements that might be used to solve the complex BC puzzle. BRCA1 was described to be the target of up to 100 miRNAs. BRCA1 may directly repress miR-155 activity. In addition, miR-15/107/182-mediated downregulation of BRCA1 interrupt DNA repair and may change the course of BC therapy. miR-146a and miR-146-5p silencing BRCA1 may trigger formation of triple-negative and basal-like sporadic BC cases. miR-182 might effect the therapy outcome. miR-21 targeted therapy might be useful for the treatment of BRCA2 mutation carriers. miR-342 overexpression and the absence of functional BRCA1 gene might cause synthetic lethality, which might be used as a base for future therapies. The present review discusses the latest data from studies that focus on the complex network of miRNAs and BRCA1/2 related BCs, which might be important for improving the therapy within the patients with triple-negative BC (TNBC) and basal-like BC, and for understanding the formation of TNBC.",
journal = "Cancer Biomarkers",
title = "MicroRNA in breast cancer: The association with BRCA1/2",
volume = "19",
number = "2",
pages = "119-128",
doi = "10.3233/CBM-160319"
}

Petrović, N., Davidović, R. S., Bajić, V. P., Obradović, M. M.,& Isenović, E. R.. (2017). MicroRNA in breast cancer: The association with BRCA1/2. in Cancer Biomarkers, 19(2), 119-128.
https://doi.org/10.3233/CBM-160319

Petrović N, Davidović RS, Bajić VP, Obradović MM, Isenović ER. MicroRNA in breast cancer: The association with BRCA1/2. in Cancer Biomarkers. 2017;19(2):119-128.
doi:10.3233/CBM-160319 .

Petrović, Nina, Davidović, Radoslav S., Bajić, Vladan P., Obradović, Milan M., Isenović, Esma R., "MicroRNA in breast cancer: The association with BRCA1/2" in Cancer Biomarkers, 19, no. 2 (2017):119-128,
https://doi.org/10.3233/CBM-160319 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Akar, Banu
AU  - Roux, Brianna M.
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan P.
AU  - Brey, Eric M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1324
AB  - Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 284-291, 2017.
T2  - Journal of Biomedical Materials Research. Part A
T1  - Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres
VL  - 105
IS  - 1
SP  - 284
EP  - 291
DO  - 10.1002/jbm.a.35849
ER  - 

@article{
author = "Živković, Lada and Akar, Banu and Roux, Brianna M. and Spremo-Potparević, Biljana and Bajić, Vladan P. and Brey, Eric M.",
year = "2017",
abstract = "Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 284-291, 2017.",
journal = "Journal of Biomedical Materials Research. Part A",
title = "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres",
volume = "105",
number = "1",
pages = "284-291",
doi = "10.1002/jbm.a.35849"
}

Živković, L., Akar, B., Roux, B. M., Spremo-Potparević, B., Bajić, V. P.,& Brey, E. M.. (2017). Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. in Journal of Biomedical Materials Research. Part A, 105(1), 284-291.
https://doi.org/10.1002/jbm.a.35849

Živković L, Akar B, Roux BM, Spremo-Potparević B, Bajić VP, Brey EM. Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. in Journal of Biomedical Materials Research. Part A. 2017;105(1):284-291.
doi:10.1002/jbm.a.35849 .

Živković, Lada, Akar, Banu, Roux, Brianna M., Spremo-Potparević, Biljana, Bajić, Vladan P., Brey, Eric M., "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres" in Journal of Biomedical Materials Research. Part A, 105, no. 1 (2017):284-291,
https://doi.org/10.1002/jbm.a.35849 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Čabarkapa, Andrea
AU  - Topalović, Dijana
AU  - Ciptasari, Ummi
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1458
AB  - The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells
DO  - 10.1155/2017/8759764
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica Z. and Čabarkapa, Andrea and Topalović, Dijana and Ciptasari, Ummi and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2017",
abstract = "The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells",
doi = "10.1155/2017/8759764"
}

Živković, L., Borozan, S. Z., Čabarkapa, A., Topalović, D., Ciptasari, U., Bajić, V. P.,& Spremo-Potparević, B.. (2017). Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity.
https://doi.org/10.1155/2017/8759764

Živković L, Borozan SZ, Čabarkapa A, Topalović D, Ciptasari U, Bajić VP, Spremo-Potparević B. Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity. 2017;.
doi:10.1155/2017/8759764 .

Živković, Lada, Borozan, Sunčica Z., Čabarkapa, Andrea, Topalović, Dijana, Ciptasari, Ummi, Bajić, Vladan P., Spremo-Potparević, Biljana, "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells" in Oxidative Medicine and Cellular Longevity (2017),
https://doi.org/10.1155/2017/8759764 . .

TY  - JOUR
AU  - Salhi, Adil
AU  - Essack, Magbubah
AU  - Alam, Tanvir
AU  - Bajić, Vladan P.
AU  - Ma, Lina
AU  - Radovanovic, Aleksandar
AU  - Marchand, Benoit
AU  - Schmeier, Sebastian
AU  - Zhang, Zhang
AU  - Bajić, Vladimir B.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1675
AB  - Noncoding RNAs (ncRNAs), particularly microRNAs (miRNAs) and long ncRNAs (lncRNAs), are important players in diseases and emerge as novel drug targets. Thus, unraveling the relationships between ncRNAs and other biomedical entities in cells are critical for better understanding ncRNA roles that may eventually help develop their use in medicine. To support ncRNA research and facilitate retrieval of relevant information regarding miRNAs and lncRNAs from the plethora of published ncRNA-related research, we developed DES-ncRNA (www.cbrc.kaust.edu.sa/des_ncrna). DES-ncRNA is a knowledgebase containing text- and data-mined information from public scientific literature and other public resources. Exploration of mined information is enabled through terms and pairs of terms from 19 topic-specific dictionaries including, for example, antibiotics, toxins, drugs, enzymes, mutations, pathways, human genes and proteins, drug indications and side effects, mutations, diseases, etc. DES-ncRNA contains approximately 878,000 associations of terms from these dictionaries of which 36,222 (5,373) are with regards to miRNAs (lncRNAs). We provide several ways to explore information regarding ncRNAs to users including controlled generation of association networks as well as hypotheses generation. We show an example how DES-ncRNA can aid research on Alzheimer disease and suggest potential therapeutic role for Fasudil. DES-ncRNA is a powerful tool that can be used on its own or as a complement to the existing resources, to support research in human ncRNA. To our knowledge, this is the only knowledgebase dedicated to human miRNAs and lncRNAs derived primarily through literature-mining enabling exploration of a broad spectrum of associated biomedical entities, not paralleled by any other resource.
T2  - RNA Biology
T1  - DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining
VL  - 14
IS  - 7
SP  - 963
EP  - 971
DO  - 10.1080/15476286.2017.1312243
ER  - 

@article{
author = "Salhi, Adil and Essack, Magbubah and Alam, Tanvir and Bajić, Vladan P. and Ma, Lina and Radovanovic, Aleksandar and Marchand, Benoit and Schmeier, Sebastian and Zhang, Zhang and Bajić, Vladimir B.",
year = "2017",
abstract = "Noncoding RNAs (ncRNAs), particularly microRNAs (miRNAs) and long ncRNAs (lncRNAs), are important players in diseases and emerge as novel drug targets. Thus, unraveling the relationships between ncRNAs and other biomedical entities in cells are critical for better understanding ncRNA roles that may eventually help develop their use in medicine. To support ncRNA research and facilitate retrieval of relevant information regarding miRNAs and lncRNAs from the plethora of published ncRNA-related research, we developed DES-ncRNA (www.cbrc.kaust.edu.sa/des_ncrna). DES-ncRNA is a knowledgebase containing text- and data-mined information from public scientific literature and other public resources. Exploration of mined information is enabled through terms and pairs of terms from 19 topic-specific dictionaries including, for example, antibiotics, toxins, drugs, enzymes, mutations, pathways, human genes and proteins, drug indications and side effects, mutations, diseases, etc. DES-ncRNA contains approximately 878,000 associations of terms from these dictionaries of which 36,222 (5,373) are with regards to miRNAs (lncRNAs). We provide several ways to explore information regarding ncRNAs to users including controlled generation of association networks as well as hypotheses generation. We show an example how DES-ncRNA can aid research on Alzheimer disease and suggest potential therapeutic role for Fasudil. DES-ncRNA is a powerful tool that can be used on its own or as a complement to the existing resources, to support research in human ncRNA. To our knowledge, this is the only knowledgebase dedicated to human miRNAs and lncRNAs derived primarily through literature-mining enabling exploration of a broad spectrum of associated biomedical entities, not paralleled by any other resource.",
journal = "RNA Biology",
title = "DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining",
volume = "14",
number = "7",
pages = "963-971",
doi = "10.1080/15476286.2017.1312243"
}

Salhi, A., Essack, M., Alam, T., Bajić, V. P., Ma, L., Radovanovic, A., Marchand, B., Schmeier, S., Zhang, Z.,& Bajić, V. B.. (2017). DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining. in RNA Biology, 14(7), 963-971.
https://doi.org/10.1080/15476286.2017.1312243

Salhi A, Essack M, Alam T, Bajić VP, Ma L, Radovanovic A, Marchand B, Schmeier S, Zhang Z, Bajić VB. DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining. in RNA Biology. 2017;14(7):963-971.
doi:10.1080/15476286.2017.1312243 .

Salhi, Adil, Essack, Magbubah, Alam, Tanvir, Bajić, Vladan P., Ma, Lina, Radovanovic, Aleksandar, Marchand, Benoit, Schmeier, Sebastian, Zhang, Zhang, Bajić, Vladimir B., "DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining" in RNA Biology, 14, no. 7 (2017):963-971,
https://doi.org/10.1080/15476286.2017.1312243 . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Dekanski, Dragana
AU  - Živković, Lada
AU  - Milanovic-Cabarkapa, Mirjana
AU  - Bajić, Vladan P.
AU  - Topalović, Dijana
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Battino, Maurizio
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1679
AB  - The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy
VL  - 106
SP  - 616
EP  - 623
DO  - 10.1016/j.fct.2016.12.023
ER  - 

@article{
author = "Čabarkapa, Andrea and Dekanski, Dragana and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Bajić, Vladan P. and Topalović, Dijana and Giampieri, Francesca and Gasparrini, Massimiliano and Battino, Maurizio and Spremo-Potparević, Biljana",
year = "2017",
abstract = "The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy",
volume = "106",
pages = "616-623",
doi = "10.1016/j.fct.2016.12.023"
}

Čabarkapa, A., Dekanski, D., Živković, L., Milanovic-Cabarkapa, M., Bajić, V. P., Topalović, D., Giampieri, F., Gasparrini, M., Battino, M.,& Spremo-Potparević, B.. (2017). Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology, 106, 616-623.
https://doi.org/10.1016/j.fct.2016.12.023

Čabarkapa A, Dekanski D, Živković L, Milanovic-Cabarkapa M, Bajić VP, Topalović D, Giampieri F, Gasparrini M, Battino M, Spremo-Potparević B. Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology. 2017;106:616-623.
doi:10.1016/j.fct.2016.12.023 .

Čabarkapa, Andrea, Dekanski, Dragana, Živković, Lada, Milanovic-Cabarkapa, Mirjana, Bajić, Vladan P., Topalović, Dijana, Giampieri, Francesca, Gasparrini, Massimiliano, Battino, Maurizio, Spremo-Potparević, Biljana, "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy" in Food and Chemical Toxicology, 106 (2017):616-623,
https://doi.org/10.1016/j.fct.2016.12.023 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Marčetić, Mirjana
AU  - Kovačević, Nada
AU  - Bajić, Vladan P.
AU  - Jovičić, Snežana
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1121
AB  - The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.
T2  - Archives of Biological Sciences
T1  - Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)
VL  - 68
IS  - 1
SP  - 135
EP  - 144
DO  - 10.2298/ABS150512135Z
ER  - 

@article{
author = "Živković, Lada and Čabarkapa, Andrea and Marčetić, Mirjana and Kovačević, Nada and Bajić, Vladan P. and Jovičić, Snežana and Spremo-Potparević, Biljana",
year = "2016",
abstract = "The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.",
journal = "Archives of Biological Sciences",
title = "Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)",
volume = "68",
number = "1",
pages = "135-144",
doi = "10.2298/ABS150512135Z"
}

Živković, L., Čabarkapa, A., Marčetić, M., Kovačević, N., Bajić, V. P., Jovičić, S.,& Spremo-Potparević, B.. (2016). Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae). in Archives of Biological Sciences, 68(1), 135-144.
https://doi.org/10.2298/ABS150512135Z

Živković L, Čabarkapa A, Marčetić M, Kovačević N, Bajić VP, Jovičić S, Spremo-Potparević B. Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae). in Archives of Biological Sciences. 2016;68(1):135-144.
doi:10.2298/ABS150512135Z .

Živković, Lada, Čabarkapa, Andrea, Marčetić, Mirjana, Kovačević, Nada, Bajić, Vladan P., Jovičić, Snežana, Spremo-Potparević, Biljana, "Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)" in Archives of Biological Sciences, 68, no. 1 (2016):135-144,
https://doi.org/10.2298/ABS150512135Z . .