TY  - JOUR
AU  - Marković, Dejan
AU  - Karadzic, Ivana
AU  - Jokanović, Vukoman R.
AU  - Vukovic, Ana
AU  - Vučić, Vesna
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1258
AB  - Millions of patients worldwide need surgery to repair or replace tissue that has been damaged through trauma or disease. To solve the problem of lost tissue, a major emphasis of tissue engineering (TE) is on tissue regeneration. Stem cells and highly porous biomaterials used as cell carriers (scaffolds) have an essential role in the production of new tissue by TE. The cellular component is important for the generation and establishment of the extracellular matrix, while a scaffold is necessary to determine the shape of the newly formed tissue and facilitate migration of cells into the desired location, as well as their growth and differentiation. This review describes the types, characteristics and classification of stem cells. Furthermore, it includes functional features of cell carriers - biocompatibility, biodegradability and mechanical properties of biomaterials used in developing state-of-the-an scaffolds for TE applications, as well as suitability for different tissues. Moreover, it explains the importance of nanotechnology and defines the challenges and the purpose of future research in this rapidly advancing field.
T2  - Chemical Industry and Chemical Engineering Quarterly / CICEQ
T1  - Biological Aspects of Application of Nanomaterials in Tissue Engineering
VL  - 22
IS  - 2
SP  - 145
EP  - 153
DO  - 10.2298/CICEQ141231028M
ER  - 

@article{
author = "Marković, Dejan and Karadzic, Ivana and Jokanović, Vukoman R. and Vukovic, Ana and Vučić, Vesna",
year = "2016",
abstract = "Millions of patients worldwide need surgery to repair or replace tissue that has been damaged through trauma or disease. To solve the problem of lost tissue, a major emphasis of tissue engineering (TE) is on tissue regeneration. Stem cells and highly porous biomaterials used as cell carriers (scaffolds) have an essential role in the production of new tissue by TE. The cellular component is important for the generation and establishment of the extracellular matrix, while a scaffold is necessary to determine the shape of the newly formed tissue and facilitate migration of cells into the desired location, as well as their growth and differentiation. This review describes the types, characteristics and classification of stem cells. Furthermore, it includes functional features of cell carriers - biocompatibility, biodegradability and mechanical properties of biomaterials used in developing state-of-the-an scaffolds for TE applications, as well as suitability for different tissues. Moreover, it explains the importance of nanotechnology and defines the challenges and the purpose of future research in this rapidly advancing field.",
journal = "Chemical Industry and Chemical Engineering Quarterly / CICEQ",
title = "Biological Aspects of Application of Nanomaterials in Tissue Engineering",
volume = "22",
number = "2",
pages = "145-153",
doi = "10.2298/CICEQ141231028M"
}

Marković, D., Karadzic, I., Jokanović, V. R., Vukovic, A.,& Vučić, V.. (2016). Biological Aspects of Application of Nanomaterials in Tissue Engineering. in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 22(2), 145-153.
https://doi.org/10.2298/CICEQ141231028M

Marković D, Karadzic I, Jokanović VR, Vukovic A, Vučić V. Biological Aspects of Application of Nanomaterials in Tissue Engineering. in Chemical Industry and Chemical Engineering Quarterly / CICEQ. 2016;22(2):145-153.
doi:10.2298/CICEQ141231028M .

Marković, Dejan, Karadzic, Ivana, Jokanović, Vukoman R., Vukovic, Ana, Vučić, Vesna, "Biological Aspects of Application of Nanomaterials in Tissue Engineering" in Chemical Industry and Chemical Engineering Quarterly / CICEQ, 22, no. 2 (2016):145-153,
https://doi.org/10.2298/CICEQ141231028M . .

TY  - JOUR
AU  - Karadzic, Ivana
AU  - Vučić, Vesna
AU  - Jokanović, Vukoman R.
AU  - Debeljak-Martačić, Jasmina
AU  - Marković, Dejan
AU  - Petrović, Snježana
AU  - Glibetić, Marija
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/235
AB  - The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.
T2  - Journal of Biomedical Materials Research. Part A
T1  - Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells
VL  - 103
IS  - 1
SP  - 350
EP  - 357
DO  - 10.1002/jbm.a.35180
ER  - 

@article{
author = "Karadzic, Ivana and Vučić, Vesna and Jokanović, Vukoman R. and Debeljak-Martačić, Jasmina and Marković, Dejan and Petrović, Snježana and Glibetić, Marija",
year = "2015",
abstract = "The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.",
journal = "Journal of Biomedical Materials Research. Part A",
title = "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells",
volume = "103",
number = "1",
pages = "350-357",
doi = "10.1002/jbm.a.35180"
}

Karadzic, I., Vučić, V., Jokanović, V. R., Debeljak-Martačić, J., Marković, D., Petrović, S.,& Glibetić, M.. (2015). Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research. Part A, 103(1), 350-357.
https://doi.org/10.1002/jbm.a.35180

Karadzic I, Vučić V, Jokanović VR, Debeljak-Martačić J, Marković D, Petrović S, Glibetić M. Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research. Part A. 2015;103(1):350-357.
doi:10.1002/jbm.a.35180 .

Karadzic, Ivana, Vučić, Vesna, Jokanović, Vukoman R., Debeljak-Martačić, Jasmina, Marković, Dejan, Petrović, Snježana, Glibetić, Marija, "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells" in Journal of Biomedical Materials Research. Part A, 103, no. 1 (2015):350-357,
https://doi.org/10.1002/jbm.a.35180 . .