TY  - JOUR
AU  - Glumac, Sofija
AU  - Davidović, Radoslav S.
AU  - Dožić, Branko
AU  - Hinić, Sasa
AU  - Pavlović, Ivan
AU  - Drakulić, Dunja R.
AU  - Todorović, Ana
AU  - Medojević Pavlović, Maja
AU  - Radojević-Škodrić, Sanja
AU  - Baralić, Ivana
AU  - Sopta, Jelena
AU  - Pejić, Snežana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9887
AB  - Rhabdomyosarcoma (RMS) is a highly malignant cancer and is the most common soft tissue sarcoma in children and adolescents, but it is rare in adults (<1% of all adult malignancies). Altered expression and molecular abnormalities of cell-cycle-regulatory proteins are one of the most prominent features in RMS. Therefore, we evaluated the expression of cyclin-dependent kinase inhibitors p57 and p16, as well as p16 methylation status, along with clinicopathological characteristics and overall survival (OS) in RMS patients. This analysis was conducted on 23 pediatric and 44 adult patients. There was a male predominance in both groups and extremities were the most frequent tumor site. In adults, alveolar and pleomorphic types were almost equally represented. The majority of pediatric tumors were low grade, whereas, in adults, only one patient had a low-grade tumor. Seven pediatric (30.43%) and eight adult (18.18%) patients had a low p16 expression. The analysis of methylation status of the p16 promoter showed the presence of methylated allele only in one sample with pleomorphic histology. Six (26.1%) pediatric and 15 (34.1%) adult patients had low p57 expression, while in 17 (73.9%) pediatric and 29 (65.9%) adult patients it was assessed as high. Ninetyone percent of the pediatric patients and 32.6% of adults were alive at the end of the observational period. In adults, significant associations were found between OS and age (P = 0.020), gender (P = 0.027), tumor size (P < 0.001), lymph node status (P < 0.001), presence of metastases (P = 0.015), and p57 expression (P = 0.039). Stratification by histological type showed the correlation of low p57 expression (P = 0.030) and worse OS of patients with alveolar RMS. Univariate analysis identified age > 50 yrs. (HR 2.447), tumors > 5 cm (HR 21.31), involvement of regional lymph nodes (HR 3.96), the presence of metastases (HR 2.53), and low p57 expression (HR 2.11) as predictors of lower OS. Tumor size, regional lymph nodes involvement, and metastases were the independent predictors after multivariate analysis, while p57 did not predict OS in an independent way. In summary, although p57 was not confirmed to be an independent predictor of OS, our results indicate that its low expression may be the marker of aggressive phenotype and poor prognosis in adult RMS patients. Also, our findings suggest that epigenetic inactivation of p16 is not important in the pathogenesis of rhabdomyosarcoma.
T2  - Pathology - Research and Practice
T1  - Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma
VL  - 225
SP  - 153558
DO  - 10.1016/j.prp.2021.153558
ER  - 

@article{
author = "Glumac, Sofija and Davidović, Radoslav S. and Dožić, Branko and Hinić, Sasa and Pavlović, Ivan and Drakulić, Dunja R. and Todorović, Ana and Medojević Pavlović, Maja and Radojević-Škodrić, Sanja and Baralić, Ivana and Sopta, Jelena and Pejić, Snežana",
year = "2021",
abstract = "Rhabdomyosarcoma (RMS) is a highly malignant cancer and is the most common soft tissue sarcoma in children and adolescents, but it is rare in adults (<1% of all adult malignancies). Altered expression and molecular abnormalities of cell-cycle-regulatory proteins are one of the most prominent features in RMS. Therefore, we evaluated the expression of cyclin-dependent kinase inhibitors p57 and p16, as well as p16 methylation status, along with clinicopathological characteristics and overall survival (OS) in RMS patients. This analysis was conducted on 23 pediatric and 44 adult patients. There was a male predominance in both groups and extremities were the most frequent tumor site. In adults, alveolar and pleomorphic types were almost equally represented. The majority of pediatric tumors were low grade, whereas, in adults, only one patient had a low-grade tumor. Seven pediatric (30.43%) and eight adult (18.18%) patients had a low p16 expression. The analysis of methylation status of the p16 promoter showed the presence of methylated allele only in one sample with pleomorphic histology. Six (26.1%) pediatric and 15 (34.1%) adult patients had low p57 expression, while in 17 (73.9%) pediatric and 29 (65.9%) adult patients it was assessed as high. Ninetyone percent of the pediatric patients and 32.6% of adults were alive at the end of the observational period. In adults, significant associations were found between OS and age (P = 0.020), gender (P = 0.027), tumor size (P < 0.001), lymph node status (P < 0.001), presence of metastases (P = 0.015), and p57 expression (P = 0.039). Stratification by histological type showed the correlation of low p57 expression (P = 0.030) and worse OS of patients with alveolar RMS. Univariate analysis identified age > 50 yrs. (HR 2.447), tumors > 5 cm (HR 21.31), involvement of regional lymph nodes (HR 3.96), the presence of metastases (HR 2.53), and low p57 expression (HR 2.11) as predictors of lower OS. Tumor size, regional lymph nodes involvement, and metastases were the independent predictors after multivariate analysis, while p57 did not predict OS in an independent way. In summary, although p57 was not confirmed to be an independent predictor of OS, our results indicate that its low expression may be the marker of aggressive phenotype and poor prognosis in adult RMS patients. Also, our findings suggest that epigenetic inactivation of p16 is not important in the pathogenesis of rhabdomyosarcoma.",
journal = "Pathology - Research and Practice",
title = "Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma",
volume = "225",
pages = "153558",
doi = "10.1016/j.prp.2021.153558"
}

Glumac, S., Davidović, R. S., Dožić, B., Hinić, S., Pavlović, I., Drakulić, D. R., Todorović, A., Medojević Pavlović, M., Radojević-Škodrić, S., Baralić, I., Sopta, J.,& Pejić, S.. (2021). Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma. in Pathology - Research and Practice, 225, 153558.
https://doi.org/10.1016/j.prp.2021.153558

Glumac S, Davidović RS, Dožić B, Hinić S, Pavlović I, Drakulić DR, Todorović A, Medojević Pavlović M, Radojević-Škodrić S, Baralić I, Sopta J, Pejić S. Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma. in Pathology - Research and Practice. 2021;225:153558.
doi:10.1016/j.prp.2021.153558 .

Glumac, Sofija, Davidović, Radoslav S., Dožić, Branko, Hinić, Sasa, Pavlović, Ivan, Drakulić, Dunja R., Todorović, Ana, Medojević Pavlović, Maja, Radojević-Škodrić, Sanja, Baralić, Ivana, Sopta, Jelena, Pejić, Snežana, "Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma" in Pathology - Research and Practice, 225 (2021):153558,
https://doi.org/10.1016/j.prp.2021.153558 . .

TY  - JOUR
AU  - Đermanović, Mirjana
AU  - Baralić, Ivana
AU  - Pejić, Snežana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8566
AB  - The mercury concentration was quantified in frozen and canned fish found in the market of the Republic of Srpska, Bosnia and Herzegovina. Using the method of amalgamation by atomic absorption spectrophotometry with a direct mercury analyzer (DMA-80), significant differences in mercury concentrations among the analyzed fish were found. Frozen pangasius and canned tuna had the highest mercury concentration (59.1 μg/kg and 54.1 μg/kg, respectively). Also, fish from Vietnam had the highest level of total mercury in comparison to fish from other countries. Determined levels of mercury in our samples were below the limit set by the European and Bosnian and Herzegovinian legislation (500 μg/kg wet weight). We have concluded that consumption of commercial fish in the market of the Republic of Srpska does not present any health risk for the average consumer. Nevertheless, a surveillance system of mercury content in fishery products, especially in certain species that concentrate mercury, is crucial for public health protection. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Biological Trace Element Research
T1  - Total Mercury Levels in Commercial Fish in Market of the Republic of Srpska, Bosnia and Herzegovina
VL  - 194
IS  - 2
SP  - 545
EP  - 551
DO  - 10.1007/s12011-019-01805-4
ER  - 

@article{
author = "Đermanović, Mirjana and Baralić, Ivana and Pejić, Snežana",
year = "2020",
abstract = "The mercury concentration was quantified in frozen and canned fish found in the market of the Republic of Srpska, Bosnia and Herzegovina. Using the method of amalgamation by atomic absorption spectrophotometry with a direct mercury analyzer (DMA-80), significant differences in mercury concentrations among the analyzed fish were found. Frozen pangasius and canned tuna had the highest mercury concentration (59.1 μg/kg and 54.1 μg/kg, respectively). Also, fish from Vietnam had the highest level of total mercury in comparison to fish from other countries. Determined levels of mercury in our samples were below the limit set by the European and Bosnian and Herzegovinian legislation (500 μg/kg wet weight). We have concluded that consumption of commercial fish in the market of the Republic of Srpska does not present any health risk for the average consumer. Nevertheless, a surveillance system of mercury content in fishery products, especially in certain species that concentrate mercury, is crucial for public health protection. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Biological Trace Element Research",
title = "Total Mercury Levels in Commercial Fish in Market of the Republic of Srpska, Bosnia and Herzegovina",
volume = "194",
number = "2",
pages = "545-551",
doi = "10.1007/s12011-019-01805-4"
}

Đermanović, M., Baralić, I.,& Pejić, S.. (2020). Total Mercury Levels in Commercial Fish in Market of the Republic of Srpska, Bosnia and Herzegovina. in Biological Trace Element Research, 194(2), 545-551.
https://doi.org/10.1007/s12011-019-01805-4

Đermanović M, Baralić I, Pejić S. Total Mercury Levels in Commercial Fish in Market of the Republic of Srpska, Bosnia and Herzegovina. in Biological Trace Element Research. 2020;194(2):545-551.
doi:10.1007/s12011-019-01805-4 .

Đermanović, Mirjana, Baralić, Ivana, Pejić, Snežana, "Total Mercury Levels in Commercial Fish in Market of the Republic of Srpska, Bosnia and Herzegovina" in Biological Trace Element Research, 194, no. 2 (2020):545-551,
https://doi.org/10.1007/s12011-019-01805-4 . .

TY  - JOUR
AU  - Radojević-Škodrić, Sanja
AU  - Brašanac, Dimitrije
AU  - Đuričić, Slaviša M.
AU  - Glumac, Sofija
AU  - Lončar, Zlatibor
AU  - Pavlović, Ivan
AU  - Todorović, Ana
AU  - Nikolić, Gorana V.
AU  - Baralić, Ivana
AU  - Pejić, Snežana
PY  - 2019
UR  - https://peerj.com/articles/6212
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8025
AB  - Background: Cyclin A overexpression is found in a variety of human tumors and correlates with unfavorable outcome. We analyzed immunohistochemical expression of cyclin A in Wilms tumor (WT) in relation to clinicopathological characteristics, preoperative chemotherapy (PrOpChTh), and overall survival (OS). Methods: This retrospective study involved 43 patients who underwent nephrectomy from January 1996 to October 2010. Tumor stage and histological subtype were determined by revised Societé International d’Oncologie Pediatrique protocol, based on histological components/alterations caused by PrOpChTh, within the prognostic group of low, intermediate and high risk, and with criteria for anaplasia. The regressive/necrotic changes in total tumor mass of primary tumor and the proportion of epithelial, blastemal, and stromal components in the remaining viable tumor tissue were also determined. Cyclin A expression was evaluated by immunohistochemistry using a polyclonal rabbit, antihuman antibody (H-432). Results: Cyclin A overexpression was found in 34.3% of WTs, with higher frequency in tumors with epithelial (31.3%) and blastemal (37.1%) components than those with stromal component (17.7%). Regarding histological type, cyclin A overexpression was found most often in focal anaplasia (100%), stromal (60%), and diffuse anaplastic (66.7) WTs. The overexpression was also more frequent in stages 3 and 4 (77.8% and 66.7%, respectively) compared to tumors in stages 1 and 2 (13.3% and 12.5%, respectively; p = 0.004) in all components, as well as in blastemal component in stages 3 and 4 (77.8% and 66.7%, respectively) vs. stages 1 and 2 (13.3% and 25%, respectively, p = 0.009). Cyclin A overexpression in all components was 66.7% in WTs with metastasis and 31.3% in WTs without metastasis (p = 0.265, Fisher test). Log-rank testing revealed differences of OS regarding stage (p = 0.000), prognostic groups (p = 0.001), and cyclin A expression in blastemal component (p = 0.025). After univariate analysis, tumor stage (p = 0.001), prognostic group (p = 0.004), and cyclin A expression in blastemal component (p = 0.042) were significant prognostic factors for OS; however, after multivariate analysis, none of these factors were confirmed as independent predictors of survival. Discussion: This study showed that cyclin A overexpression might be associated with the development and progression of WT with anaplasia. Also, cyclin A overexpression was more often observed in advanced stages (3 and 4) of WT, in the group of high-risk WTs, and in focal and diffuse anaplasia WTs. There was no relation of cyclin A overexpression and metastatic ability of WT. Although this study has not confirmed the prognostic value of cyclin A overexpression, its association with unfavorable prognosis should be further evaluated. Copyright 2019 Radojevic-Škodric et al.
T2  - PeerJ
T1  - Immunohistochemical analysis of cyclin A expression in Wilms tumor
VL  - 6
IS  - 1
SP  - e6212
DO  - 10.7717/peerj.6212
ER  - 

@article{
author = "Radojević-Škodrić, Sanja and Brašanac, Dimitrije and Đuričić, Slaviša M. and Glumac, Sofija and Lončar, Zlatibor and Pavlović, Ivan and Todorović, Ana and Nikolić, Gorana V. and Baralić, Ivana and Pejić, Snežana",
year = "2019",
abstract = "Background: Cyclin A overexpression is found in a variety of human tumors and correlates with unfavorable outcome. We analyzed immunohistochemical expression of cyclin A in Wilms tumor (WT) in relation to clinicopathological characteristics, preoperative chemotherapy (PrOpChTh), and overall survival (OS). Methods: This retrospective study involved 43 patients who underwent nephrectomy from January 1996 to October 2010. Tumor stage and histological subtype were determined by revised Societé International d’Oncologie Pediatrique protocol, based on histological components/alterations caused by PrOpChTh, within the prognostic group of low, intermediate and high risk, and with criteria for anaplasia. The regressive/necrotic changes in total tumor mass of primary tumor and the proportion of epithelial, blastemal, and stromal components in the remaining viable tumor tissue were also determined. Cyclin A expression was evaluated by immunohistochemistry using a polyclonal rabbit, antihuman antibody (H-432). Results: Cyclin A overexpression was found in 34.3% of WTs, with higher frequency in tumors with epithelial (31.3%) and blastemal (37.1%) components than those with stromal component (17.7%). Regarding histological type, cyclin A overexpression was found most often in focal anaplasia (100%), stromal (60%), and diffuse anaplastic (66.7) WTs. The overexpression was also more frequent in stages 3 and 4 (77.8% and 66.7%, respectively) compared to tumors in stages 1 and 2 (13.3% and 12.5%, respectively; p = 0.004) in all components, as well as in blastemal component in stages 3 and 4 (77.8% and 66.7%, respectively) vs. stages 1 and 2 (13.3% and 25%, respectively, p = 0.009). Cyclin A overexpression in all components was 66.7% in WTs with metastasis and 31.3% in WTs without metastasis (p = 0.265, Fisher test). Log-rank testing revealed differences of OS regarding stage (p = 0.000), prognostic groups (p = 0.001), and cyclin A expression in blastemal component (p = 0.025). After univariate analysis, tumor stage (p = 0.001), prognostic group (p = 0.004), and cyclin A expression in blastemal component (p = 0.042) were significant prognostic factors for OS; however, after multivariate analysis, none of these factors were confirmed as independent predictors of survival. Discussion: This study showed that cyclin A overexpression might be associated with the development and progression of WT with anaplasia. Also, cyclin A overexpression was more often observed in advanced stages (3 and 4) of WT, in the group of high-risk WTs, and in focal and diffuse anaplasia WTs. There was no relation of cyclin A overexpression and metastatic ability of WT. Although this study has not confirmed the prognostic value of cyclin A overexpression, its association with unfavorable prognosis should be further evaluated. Copyright 2019 Radojevic-Škodric et al.",
journal = "PeerJ",
title = "Immunohistochemical analysis of cyclin A expression in Wilms tumor",
volume = "6",
number = "1",
pages = "e6212",
doi = "10.7717/peerj.6212"
}

Radojević-Škodrić, S., Brašanac, D., Đuričić, S. M., Glumac, S., Lončar, Z., Pavlović, I., Todorović, A., Nikolić, G. V., Baralić, I.,& Pejić, S.. (2019). Immunohistochemical analysis of cyclin A expression in Wilms tumor. in PeerJ, 6(1), e6212.
https://doi.org/10.7717/peerj.6212

Radojević-Škodrić S, Brašanac D, Đuričić SM, Glumac S, Lončar Z, Pavlović I, Todorović A, Nikolić GV, Baralić I, Pejić S. Immunohistochemical analysis of cyclin A expression in Wilms tumor. in PeerJ. 2019;6(1):e6212.
doi:10.7717/peerj.6212 .

Radojević-Škodrić, Sanja, Brašanac, Dimitrije, Đuričić, Slaviša M., Glumac, Sofija, Lončar, Zlatibor, Pavlović, Ivan, Todorović, Ana, Nikolić, Gorana V., Baralić, Ivana, Pejić, Snežana, "Immunohistochemical analysis of cyclin A expression in Wilms tumor" in PeerJ, 6, no. 1 (2019):e6212,
https://doi.org/10.7717/peerj.6212 . .

TY  - JOUR
AU  - Baralić, Ivana
AU  - Stojmenović, Tamara
AU  - Anđelković, Marija
AU  - Đorđević, Brižita
AU  - Dikić, Nenad
AU  - Radojević-Škodrić, Sanja
AU  - Pejić, Snežana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8792
AB  - Introduction/Objective We determined the impact of antioxidant supplementation by GE132® on sports performance, oxidative stress markers, and muscle enzymes activities in professional female basketball players. Methods Repetitive strength, explosive power, anaerobic endurance, and agility performance were measured before/after the 45-day supplementation period. The FORT (Free Oxygen Radicals Test) and FORD analysis (Free Oxygen Radical Defense) were assessed before/after basketball specific exercise bout, at the beginning/end of observational period. The grade of muscle damage was evaluated by aspartate aminotransferase (AST), creatine kinase (CK) and lactate dehydrogenase (LDH). Results After supplementation period, significant difference was not recorded regarding the basic motor skills tests. Basketball specific exercise bout induced significant increase in FORT (p < 0.05) only at the beginning of supplementation period. Both FORT and FORD significantly decreased over the observational period (p < 0.001, p < 0.01, respectively). CK and LDH were remarkably lower at the end of observational period (p < 0.05), compared to the baseline. Conclusion Exogenous supplementation with protective nutraceuticals such as those found in GE132®, could reduce acute/chronic oxidative stress and muscle damage, but had no effect on sport performance in basketball players. © 2019, Serbia Medical Society. All rights reserved.
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Effect of combined antioxidant treatment on oxidative stress, muscle damage and sport performance in female basketball players
VL  - 147
IS  - 11-12
SP  - 729
EP  - 735
DO  - 10.2298/SARH190118063B
ER  - 

@article{
author = "Baralić, Ivana and Stojmenović, Tamara and Anđelković, Marija and Đorđević, Brižita and Dikić, Nenad and Radojević-Škodrić, Sanja and Pejić, Snežana",
year = "2019",
abstract = "Introduction/Objective We determined the impact of antioxidant supplementation by GE132® on sports performance, oxidative stress markers, and muscle enzymes activities in professional female basketball players. Methods Repetitive strength, explosive power, anaerobic endurance, and agility performance were measured before/after the 45-day supplementation period. The FORT (Free Oxygen Radicals Test) and FORD analysis (Free Oxygen Radical Defense) were assessed before/after basketball specific exercise bout, at the beginning/end of observational period. The grade of muscle damage was evaluated by aspartate aminotransferase (AST), creatine kinase (CK) and lactate dehydrogenase (LDH). Results After supplementation period, significant difference was not recorded regarding the basic motor skills tests. Basketball specific exercise bout induced significant increase in FORT (p < 0.05) only at the beginning of supplementation period. Both FORT and FORD significantly decreased over the observational period (p < 0.001, p < 0.01, respectively). CK and LDH were remarkably lower at the end of observational period (p < 0.05), compared to the baseline. Conclusion Exogenous supplementation with protective nutraceuticals such as those found in GE132®, could reduce acute/chronic oxidative stress and muscle damage, but had no effect on sport performance in basketball players. © 2019, Serbia Medical Society. All rights reserved.",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Effect of combined antioxidant treatment on oxidative stress, muscle damage and sport performance in female basketball players",
volume = "147",
number = "11-12",
pages = "729-735",
doi = "10.2298/SARH190118063B"
}

Baralić, I., Stojmenović, T., Anđelković, M., Đorđević, B., Dikić, N., Radojević-Škodrić, S.,& Pejić, S.. (2019). Effect of combined antioxidant treatment on oxidative stress, muscle damage and sport performance in female basketball players. in Srpski arhiv za celokupno lekarstvo, 147(11-12), 729-735.
https://doi.org/10.2298/SARH190118063B

Baralić I, Stojmenović T, Anđelković M, Đorđević B, Dikić N, Radojević-Škodrić S, Pejić S. Effect of combined antioxidant treatment on oxidative stress, muscle damage and sport performance in female basketball players. in Srpski arhiv za celokupno lekarstvo. 2019;147(11-12):729-735.
doi:10.2298/SARH190118063B .

Baralić, Ivana, Stojmenović, Tamara, Anđelković, Marija, Đorđević, Brižita, Dikić, Nenad, Radojević-Škodrić, Sanja, Pejić, Snežana, "Effect of combined antioxidant treatment on oxidative stress, muscle damage and sport performance in female basketball players" in Srpski arhiv za celokupno lekarstvo, 147, no. 11-12 (2019):729-735,
https://doi.org/10.2298/SARH190118063B . .