TY  - CONF
AU  - Žakula, Jelena
AU  - Popović, Nataša
AU  - Mićević, Mirjana
AU  - Aškrabić, Sonja
AU  - Stojadinović, Bojan
AU  - Korićanac, Lela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12640
AB  - Background: Cancer nanomedicine is a rapidly developing fi eld that uses nanoparƟ cles (NPs) for the diagnosis and treatment of cancer. Currently, many nanomaterials with diff erent shapes, sizes, structures, and composiƟ ons have been invesƟ gated to produce eff ecƟ ve anƟ cancer NPs. The interest in the biomedical applicaƟ ons of bismuth-containing nanoparƟ cles, such as bismuth ferrite (BFO-NP) is a result of their promising properƟ es such as cost-effecƟ veness, chemical inertness, high stability, and simplicity of funcƟ onalizaƟ on. Material and Methods: A375 human melanoma and HeLa cervical carcinoma cells were used to study the anƟ tumor acƟ vity of BFO-NP. Clonogenicity of treated cells was analyzed by colony forming assay, while cell death was examined using fl ow cytometry. DCF-DA fl uorescent assay was applied to measure ROS producƟ on. Protein expression of p62 and TfR1 was detected by Western blot. Cell migraƟ on was analyzed using a wound scratch assay, while an SRB assay was used to assess cell adhesion. Results: BFO-NP (200 ng/L) signifi cantly reduced the clonogenicity of A375 and HeLa cells by 46 and 60%, respecƟ vely. Detected ROS producƟ on was increased considerably, especially for A375 melanoma cells, and amounted to 400%. The number of late apoptoƟ c and/or necroƟ c cells increased by 10-12%, compared to the control. Signifi cantly increased expression of autophagy-related protein p62 was observed in both cell lines aŌ er BFO-NP treatment. Ferroptosis-related transferrin receptor (TfR1) expression was slightly increased in treated A375 end HeLa cells (~14%). The noƟ ced increase in cell adhesion ranged from 20-30% followed by a decrease in cell migraƟ on. Conclusion:BFO-NP is a promising anƟ tumor agent with a signifi cant inhibitory eff ect on A375 and HeLa cell growth and metastaƟ c potenƟ al. Molecular mechanisms involved in these processes include ROS producƟ on and increased p62 expression. Reduced metastaƟ c potenƟ al resulted from the inducƟ onof cell adhesion and decreased cell migraƟ on.
PB  - Belgrade : Serbian Association for Cancer Research
C3  - Oncology Insights
T1  - Bismuth ferrite nanoparticles increase ROS production and p62 expression in A375 melanoma and HeLa cells
IS  - 1
SP  - 100
EP  - 101
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12640
ER  - 

@conference{
author = "Žakula, Jelena and Popović, Nataša and Mićević, Mirjana and Aškrabić, Sonja and Stojadinović, Bojan and Korićanac, Lela",
year = "2023",
abstract = "Background: Cancer nanomedicine is a rapidly developing fi eld that uses nanoparƟ cles (NPs) for the diagnosis and treatment of cancer. Currently, many nanomaterials with diff erent shapes, sizes, structures, and composiƟ ons have been invesƟ gated to produce eff ecƟ ve anƟ cancer NPs. The interest in the biomedical applicaƟ ons of bismuth-containing nanoparƟ cles, such as bismuth ferrite (BFO-NP) is a result of their promising properƟ es such as cost-effecƟ veness, chemical inertness, high stability, and simplicity of funcƟ onalizaƟ on. Material and Methods: A375 human melanoma and HeLa cervical carcinoma cells were used to study the anƟ tumor acƟ vity of BFO-NP. Clonogenicity of treated cells was analyzed by colony forming assay, while cell death was examined using fl ow cytometry. DCF-DA fl uorescent assay was applied to measure ROS producƟ on. Protein expression of p62 and TfR1 was detected by Western blot. Cell migraƟ on was analyzed using a wound scratch assay, while an SRB assay was used to assess cell adhesion. Results: BFO-NP (200 ng/L) signifi cantly reduced the clonogenicity of A375 and HeLa cells by 46 and 60%, respecƟ vely. Detected ROS producƟ on was increased considerably, especially for A375 melanoma cells, and amounted to 400%. The number of late apoptoƟ c and/or necroƟ c cells increased by 10-12%, compared to the control. Signifi cantly increased expression of autophagy-related protein p62 was observed in both cell lines aŌ er BFO-NP treatment. Ferroptosis-related transferrin receptor (TfR1) expression was slightly increased in treated A375 end HeLa cells (~14%). The noƟ ced increase in cell adhesion ranged from 20-30% followed by a decrease in cell migraƟ on. Conclusion:BFO-NP is a promising anƟ tumor agent with a signifi cant inhibitory eff ect on A375 and HeLa cell growth and metastaƟ c potenƟ al. Molecular mechanisms involved in these processes include ROS producƟ on and increased p62 expression. Reduced metastaƟ c potenƟ al resulted from the inducƟ onof cell adhesion and decreased cell migraƟ on.",
publisher = "Belgrade : Serbian Association for Cancer Research",
journal = "Oncology Insights",
title = "Bismuth ferrite nanoparticles increase ROS production and p62 expression in A375 melanoma and HeLa cells",
number = "1",
pages = "100-101",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12640"
}

Žakula, J., Popović, N., Mićević, M., Aškrabić, S., Stojadinović, B.,& Korićanac, L.. (2023). Bismuth ferrite nanoparticles increase ROS production and p62 expression in A375 melanoma and HeLa cells. in Oncology Insights
Belgrade : Serbian Association for Cancer Research.(1), 100-101.
https://hdl.handle.net/21.15107/rcub_vinar_12640

Žakula J, Popović N, Mićević M, Aškrabić S, Stojadinović B, Korićanac L. Bismuth ferrite nanoparticles increase ROS production and p62 expression in A375 melanoma and HeLa cells. in Oncology Insights. 2023;(1):100-101.
https://hdl.handle.net/21.15107/rcub_vinar_12640 .

Žakula, Jelena, Popović, Nataša, Mićević, Mirjana, Aškrabić, Sonja, Stojadinović, Bojan, Korićanac, Lela, "Bismuth ferrite nanoparticles increase ROS production and p62 expression in A375 melanoma and HeLa cells" in Oncology Insights, no. 1 (2023):100-101,
https://hdl.handle.net/21.15107/rcub_vinar_12640 .