@conference{
author = "Ademović, Nejla and Tomić, Tijana and Tanić, Nasta and Milić, Marina and Rakić, Miodrag and Tanić, Nikola",
year = "2023",
abstract = "Introduction: Glioblastoma and Astrocytoma are diff use malignant brain tumors and characterized as the most aggressive and invasive brain cancers. Glioblastoma IDH wild-type is a primary brain tumour that develops de novo, and Astrocytoma IDH mutant is a secondary tumour which arises by progression from lower tumour grades. They are characterized by poor survival, resistance to therapy and poor prognosis which develops as a consequence of genomic instability. Genomic instability also contributes to tumour heterogeneity and provides the genomic diversity necessary for selecƟ on. Materials and methods: 31 paƟ ents with Glioblastoma IDH wild-type and Astrocytoma IDH mutant, grade 3 and 4, were analysed for the presence of genomic instability using AP-PCR, DNA profi ling method. Comparing DNA profi les between tumour Ɵ ssue and normal Ɵ ssue (blood) of the same paƟ ent, we detected qualitaƟ ve and quanƟ taƟ ve changes. QualitaƟ ve changes are detected as the presence and absence of bands and are the manifestaƟ on of microsatellite instability (MIN). QuanƟ taƟ ve changes are the representaƟ on of chromosomal instability (CIN) and are detected as diff erences in the intensity of bands. Survival analyses were performed using Kaplan & Maier test for survival data in relaƟ on to diff erent histological tumour type and genomic instability. StaƟ sƟ cal diff erences were considered signifi cant for p≤ 0,05. Results: PaƟ ents with Glioblastoma IDH wild-type have signifi cantly shorter survival compared to other histological types (p=0,025). For each histological type that we analysed and each type of instability MIN, CIN and total genomic instability, two groups of paƟ ents were made – those with high and low instability. PaƟ ents with Glioblastoma IDH wild-type that have low total genomic instability have signifi cantly shorter survival (p=0,045) compared to other analysed types of brain cancer. PaƟ ents with Astrocytoma IDH mutant grade 4 who have high total genomic instability and high CIN have signifi cantly shorter survival (p=0,018, p=0,007 respecƞ ully). Conclusion: PaƟ ents with Glioblastoma IDH wild-type have shorter survival which makes this tumour the most aggressive and malignat of all analysed tumours. Our results show that low genomic instability in Glioblastoma IDH wild-type and high genomic instability lead by high CIN in Astrocytoma IDH mutant, gradus 4 contribute to shorter survival, which makes genomic instability a potential good prognostic marker",
publisher = "Belgrade : Serbian Association for Cancer Research",
journal = "Oncology Insights",
title = "Genomic instability as a prognostic marker in malignant brain cancer",
number = "1",
pages = "90-91",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12638"
}
