TY  - CONF
AU  - Miletić, M.
AU  - Aškrabić, S.
AU  - Schie, I.
AU  - Rüger, J.
AU  - Korićanac, Lela
AU  - Mondol, A. S.
AU  - Vasić, Borislav
AU  - Dohčević-Mitrović, Zorana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11881
AB  - Study of the interaction between nanoparticles and human cells is usually performed using customized biochemical assays that mostly offer measurements of a single quantity/property and use labels. Raman spectroscopy on the other hand offers integral insight into complex information on biomolecular composition and molecule conformation inside cells by measuring vibrational spectra from the entire cell [1]. Furthermore, it does not require dyes nor other labels and sample preparation is very simple, which reduces time consumation and possibility of cell damage during preparation. Cerium-dioxide (CeO2) nanoparticles are known for their controversial dual activity in numerous studied cancer cell lines: while protecting some cell types from oxidative damage, their cytotoxic effect in other cell lines is also reported [2, 3]. Here, effects of two types of CeO2 nanoparticles: uncoated and dextran-coated, were studied in HeLa cells, a cervical carcinoma derived cell line. Nanoparticle-treated cells were probed by routinely used biological assays for cell growth and viability, based on dying with Sulforhodamine B and Trypan Blue, respectively [3]. The tests have shown that the nanoparticles have more prominent effect on cell growth than on viability. In the light of this information Raman spectroscopy was employed in order to investigate the changes in biomolecular content of the cervical cancer cells after treatment with nanoparticles and find connection between these changes and the resulting cell status. Raman spectra of nanoparticletreated and control (untreated) cells were obtained using 532 nm laser line as an excitation probe. From each experimental group, at least 250 cell spectra were measured. Principal component analysis (PCA) covering the spectral regions (700-1800) cm-1 and (2800-3200) cm-1 has extracted the differences between vibrational spectra features of nanoparticle-treated and control cells, but also between spectra of cells treated with uncoated and coated CeO2 nanoparticles. These changes have been associated with induced alterations of prominent groups of biomolecules, DNA, lipids and proteins. Reduced total DNA content and/or breaking of O-P-O bonds leads to the decreased vibrational intensity of 785 cm-1 peak which differentiates to a large degree treated and control cells. Amide I vibrational band (1600-1670) cm-1 , characteristic for peptide bonds and modulated by proteins secondary structure, differentiates between cells treated with coated and uncoated nanoparticles. Correlation of the spectral information with the results of biological assays was performed.
PB  - Belgrade : Vinča Institute of Nuclear Sciences, University of Belgrade
C3  - PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts
T1  - Effects of cerium-dioxide nanoparticles in cervical cancer cells studied by Raman spectroscopy
SP  - 132
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11881
ER  - 

@conference{
author = "Miletić, M. and Aškrabić, S. and Schie, I. and Rüger, J. and Korićanac, Lela and Mondol, A. S. and Vasić, Borislav and Dohčević-Mitrović, Zorana",
year = "2019",
abstract = "Study of the interaction between nanoparticles and human cells is usually performed using customized biochemical assays that mostly offer measurements of a single quantity/property and use labels. Raman spectroscopy on the other hand offers integral insight into complex information on biomolecular composition and molecule conformation inside cells by measuring vibrational spectra from the entire cell [1]. Furthermore, it does not require dyes nor other labels and sample preparation is very simple, which reduces time consumation and possibility of cell damage during preparation. Cerium-dioxide (CeO2) nanoparticles are known for their controversial dual activity in numerous studied cancer cell lines: while protecting some cell types from oxidative damage, their cytotoxic effect in other cell lines is also reported [2, 3]. Here, effects of two types of CeO2 nanoparticles: uncoated and dextran-coated, were studied in HeLa cells, a cervical carcinoma derived cell line. Nanoparticle-treated cells were probed by routinely used biological assays for cell growth and viability, based on dying with Sulforhodamine B and Trypan Blue, respectively [3]. The tests have shown that the nanoparticles have more prominent effect on cell growth than on viability. In the light of this information Raman spectroscopy was employed in order to investigate the changes in biomolecular content of the cervical cancer cells after treatment with nanoparticles and find connection between these changes and the resulting cell status. Raman spectra of nanoparticletreated and control (untreated) cells were obtained using 532 nm laser line as an excitation probe. From each experimental group, at least 250 cell spectra were measured. Principal component analysis (PCA) covering the spectral regions (700-1800) cm-1 and (2800-3200) cm-1 has extracted the differences between vibrational spectra features of nanoparticle-treated and control cells, but also between spectra of cells treated with uncoated and coated CeO2 nanoparticles. These changes have been associated with induced alterations of prominent groups of biomolecules, DNA, lipids and proteins. Reduced total DNA content and/or breaking of O-P-O bonds leads to the decreased vibrational intensity of 785 cm-1 peak which differentiates to a large degree treated and control cells. Amide I vibrational band (1600-1670) cm-1 , characteristic for peptide bonds and modulated by proteins secondary structure, differentiates between cells treated with coated and uncoated nanoparticles. Correlation of the spectral information with the results of biological assays was performed.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts",
title = "Effects of cerium-dioxide nanoparticles in cervical cancer cells studied by Raman spectroscopy",
pages = "132-132",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11881"
}

Miletić, M., Aškrabić, S., Schie, I., Rüger, J., Korićanac, L., Mondol, A. S., Vasić, B.,& Dohčević-Mitrović, Z.. (2019). Effects of cerium-dioxide nanoparticles in cervical cancer cells studied by Raman spectroscopy. in PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts
Belgrade : Vinča Institute of Nuclear Sciences, University of Belgrade., 132-132.
https://hdl.handle.net/21.15107/rcub_vinar_11881

Miletić M, Aškrabić S, Schie I, Rüger J, Korićanac L, Mondol AS, Vasić B, Dohčević-Mitrović Z. Effects of cerium-dioxide nanoparticles in cervical cancer cells studied by Raman spectroscopy. in PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts. 2019;:132-132.
https://hdl.handle.net/21.15107/rcub_vinar_11881 .

Miletić, M., Aškrabić, S., Schie, I., Rüger, J., Korićanac, Lela, Mondol, A. S., Vasić, Borislav, Dohčević-Mitrović, Zorana, "Effects of cerium-dioxide nanoparticles in cervical cancer cells studied by Raman spectroscopy" in PHOTONICA2019 : 7th International School and Conference on Photonics & Machine Learning with Photonics Symposium : Book of abstracts (2019):132-132,
https://hdl.handle.net/21.15107/rcub_vinar_11881 .

TY  - CONF
AU  - Isić, G.
AU  - Ralević, U.
AU  - Aškrabić, S.
AU  - Laban, B.
AU  - Vodnik, Vesna
AU  - Vasić, Vesna
AU  - Gajić, R.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12539
AB  - Silver nanoparticles (AgNPs) capped with dye molecules and their J-aggregates often have remarkable optical properties that can lead to applications ranging from nanoscale sensing, light harvestering and bio-labelling to advanced composite materials for novel active and nonlinear optical devices. Here we investigate the J-aggregation of a thiacyanine dye (TC) on the surface of citrate capped AgNP nanoasseblies in which the average diameter of individual AgNPs is around 10 nm. Combining Raman mapping, surface-enhanced Raman spectroscopy (SERS) and atomic force microscopy (AFM), we study the influence of TC concentration on its J-aggregation on AgNPs surface. The TC – AgNPs assemblies were deposited onto freshly cleaved highly oriented pyrolytic graphite and mica surfaces. The spectral signature of citrate ions is identified by (i) the O-H band around 220 cm-1, (ii) the C-H band around 2960 cm-1 and (iii) pronounced blinking in the 1000-1800 cm-1 range. In contrast, dye molecules adsorbed on the nanoparticles are recognized by several stable Raman bands between 200 and 1600 cm-1. In situ AFM measurements indicate that the 'hot spots' are formed either on large single nanoparticles (diameter > 100 nm) or within aggregates of small nanoparticles (with diameters in the 10 - 50 nm range). However, only the latter are found to yield a citrate or TC dye SERS signal. Our study indicates that even in highly concentrated dye solutions, some citrate ions remain attached to the nanoparticles.
C3  - 2nd Optical Nanospectroscopy Conference : Conference book
T1  - Study of thiacyanine dye J-aggregates on single silver nanoparticle assemblies by surface enhanced Raman scattering and atomic force microscopy
SP  - 37
EP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12539
ER  - 

@conference{
author = "Isić, G. and Ralević, U. and Aškrabić, S. and Laban, B. and Vodnik, Vesna and Vasić, Vesna and Gajić, R.",
year = "2015",
abstract = "Silver nanoparticles (AgNPs) capped with dye molecules and their J-aggregates often have remarkable optical properties that can lead to applications ranging from nanoscale sensing, light harvestering and bio-labelling to advanced composite materials for novel active and nonlinear optical devices. Here we investigate the J-aggregation of a thiacyanine dye (TC) on the surface of citrate capped AgNP nanoasseblies in which the average diameter of individual AgNPs is around 10 nm. Combining Raman mapping, surface-enhanced Raman spectroscopy (SERS) and atomic force microscopy (AFM), we study the influence of TC concentration on its J-aggregation on AgNPs surface. The TC – AgNPs assemblies were deposited onto freshly cleaved highly oriented pyrolytic graphite and mica surfaces. The spectral signature of citrate ions is identified by (i) the O-H band around 220 cm-1, (ii) the C-H band around 2960 cm-1 and (iii) pronounced blinking in the 1000-1800 cm-1 range. In contrast, dye molecules adsorbed on the nanoparticles are recognized by several stable Raman bands between 200 and 1600 cm-1. In situ AFM measurements indicate that the 'hot spots' are formed either on large single nanoparticles (diameter > 100 nm) or within aggregates of small nanoparticles (with diameters in the 10 - 50 nm range). However, only the latter are found to yield a citrate or TC dye SERS signal. Our study indicates that even in highly concentrated dye solutions, some citrate ions remain attached to the nanoparticles.",
journal = "2nd Optical Nanospectroscopy Conference : Conference book",
title = "Study of thiacyanine dye J-aggregates on single silver nanoparticle assemblies by surface enhanced Raman scattering and atomic force microscopy",
pages = "37-37",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12539"
}

Isić, G., Ralević, U., Aškrabić, S., Laban, B., Vodnik, V., Vasić, V.,& Gajić, R.. (2015). Study of thiacyanine dye J-aggregates on single silver nanoparticle assemblies by surface enhanced Raman scattering and atomic force microscopy. in 2nd Optical Nanospectroscopy Conference : Conference book, 37-37.
https://hdl.handle.net/21.15107/rcub_vinar_12539

Isić G, Ralević U, Aškrabić S, Laban B, Vodnik V, Vasić V, Gajić R. Study of thiacyanine dye J-aggregates on single silver nanoparticle assemblies by surface enhanced Raman scattering and atomic force microscopy. in 2nd Optical Nanospectroscopy Conference : Conference book. 2015;:37-37.
https://hdl.handle.net/21.15107/rcub_vinar_12539 .

Isić, G., Ralević, U., Aškrabić, S., Laban, B., Vodnik, Vesna, Vasić, Vesna, Gajić, R., "Study of thiacyanine dye J-aggregates on single silver nanoparticle assemblies by surface enhanced Raman scattering and atomic force microscopy" in 2nd Optical Nanospectroscopy Conference : Conference book (2015):37-37,
https://hdl.handle.net/21.15107/rcub_vinar_12539 .