TY  - JOUR
AU  - Popović, Nataša M.
AU  - Ruždijić, Sabera
AU  - Kanazir, Dušan T.
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Paraskevopoulou, Elissavet
AU  - Pantelidou, Constantia
AU  - Radojčić, Marija
AU  - Demonacos, Constantinos
AU  - Krstić-Demonacos, Marija
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3999
AB  - The glucocorticoid receptor (GR) signal transduction and transcriptional regulation are efficiently recapitulated when GR is expressed in Saccharomyces cerevisiae. In this report we demonstrate that the in vivo GR phosphorylation pattern, hormone dependency and interdependency of phosphorylation events were similar in yeast and mammalian cells. GR phosphorylation at S246 exhibited inhibitory effect on S224 and S232 phosphorylation, suggesting the conservation of molecular mechanisms that control this interdependence between yeast and mammalian cells. To assess the effects of GR phosphorylation the mutated GR derivatives T171A, S224A, S232A, S246A were overexpressed and their transcriptional activity was analysed. These receptor derivatives displayed significant hormone inducible transcription when overexpressed in S. cerevisiae. We have established an inducible methionine expression system, which allows the close regulation of the receptor protein levels to analyse the dependence of GR function on its phosphorylation and protein abundance. Using this system we observed that GR S246A mutation increased its activity across all of the GR concentrations tested. The activity of the S224A and S246A mutants was mostly independent of GR protein levels, whereas the WT, T171A and S232A mediated transcription diminished with declining GR protein levels. Our results suggest that GR phosphorylation at specific residues affects its transcriptional functions in a site selective manner and these effects were directly linked to GR dosage. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
T2  - Steroids
T1  - Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae
VL  - 75
IS  - 6
SP  - 457
EP  - 465
DO  - 10.1016/j.steroids.2010.03.001
ER  - 

@article{
author = "Popović, Nataša M. and Ruždijić, Sabera and Kanazir, Dušan T. and Nićiforović, Ana and Adžić, Miroslav and Paraskevopoulou, Elissavet and Pantelidou, Constantia and Radojčić, Marija and Demonacos, Constantinos and Krstić-Demonacos, Marija",
year = "2010",
abstract = "The glucocorticoid receptor (GR) signal transduction and transcriptional regulation are efficiently recapitulated when GR is expressed in Saccharomyces cerevisiae. In this report we demonstrate that the in vivo GR phosphorylation pattern, hormone dependency and interdependency of phosphorylation events were similar in yeast and mammalian cells. GR phosphorylation at S246 exhibited inhibitory effect on S224 and S232 phosphorylation, suggesting the conservation of molecular mechanisms that control this interdependence between yeast and mammalian cells. To assess the effects of GR phosphorylation the mutated GR derivatives T171A, S224A, S232A, S246A were overexpressed and their transcriptional activity was analysed. These receptor derivatives displayed significant hormone inducible transcription when overexpressed in S. cerevisiae. We have established an inducible methionine expression system, which allows the close regulation of the receptor protein levels to analyse the dependence of GR function on its phosphorylation and protein abundance. Using this system we observed that GR S246A mutation increased its activity across all of the GR concentrations tested. The activity of the S224A and S246A mutants was mostly independent of GR protein levels, whereas the WT, T171A and S232A mediated transcription diminished with declining GR protein levels. Our results suggest that GR phosphorylation at specific residues affects its transcriptional functions in a site selective manner and these effects were directly linked to GR dosage. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.",
journal = "Steroids",
title = "Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae",
volume = "75",
number = "6",
pages = "457-465",
doi = "10.1016/j.steroids.2010.03.001"
}

Popović, N. M., Ruždijić, S., Kanazir, D. T., Nićiforović, A., Adžić, M., Paraskevopoulou, E., Pantelidou, C., Radojčić, M., Demonacos, C.,& Krstić-Demonacos, M.. (2010). Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae. in Steroids, 75(6), 457-465.
https://doi.org/10.1016/j.steroids.2010.03.001

Popović NM, Ruždijić S, Kanazir DT, Nićiforović A, Adžić M, Paraskevopoulou E, Pantelidou C, Radojčić M, Demonacos C, Krstić-Demonacos M. Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae. in Steroids. 2010;75(6):457-465.
doi:10.1016/j.steroids.2010.03.001 .

Popović, Nataša M., Ruždijić, Sabera, Kanazir, Dušan T., Nićiforović, Ana, Adžić, Miroslav, Paraskevopoulou, Elissavet, Pantelidou, Constantia, Radojčić, Marija, Demonacos, Constantinos, Krstić-Demonacos, Marija, "Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae" in Steroids, 75, no. 6 (2010):457-465,
https://doi.org/10.1016/j.steroids.2010.03.001 . .

TY  - JOUR
AU  - Vučić, Vesna
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3467
AB  - The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.
T2  - Investigational New Drugs
T1  - The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells
VL  - 26
IS  - 4
SP  - 309
EP  - 317
DO  - 10.1007/s10637-007-9101-4
ER  - 

@article{
author = "Vučić, Vesna and Nićiforović, Ana and Adžić, Miroslav and Radojčić, Marija and Ruždijić, Sabera",
year = "2008",
abstract = "The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.",
journal = "Investigational New Drugs",
title = "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells",
volume = "26",
number = "4",
pages = "309-317",
doi = "10.1007/s10637-007-9101-4"
}

Vučić, V., Nićiforović, A., Adžić, M., Radojčić, M.,& Ruždijić, S.. (2008). The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs, 26(4), 309-317.
https://doi.org/10.1007/s10637-007-9101-4

Vučić V, Nićiforović A, Adžić M, Radojčić M, Ruždijić S. The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs. 2008;26(4):309-317.
doi:10.1007/s10637-007-9101-4 .

Vučić, Vesna, Nićiforović, Ana, Adžić, Miroslav, Radojčić, Marija, Ruždijić, Sabera, "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells" in Investigational New Drugs, 26, no. 4 (2008):309-317,
https://doi.org/10.1007/s10637-007-9101-4 . .

TY  - JOUR
AU  - Vujčić, Miroslava T.
AU  - Velickovic, Natasa
AU  - Ruždijić, Sabera
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3326
AB  - Aging is associated with marked changes in the biochemical processes of many organs. Basal and glucocorticoid induced of liver nuclear glucocorticoid receptor (GR) on the level of protein expression and DNA-binding activity were investigated at different ages (3, 6, 12, 18 and 24 months old) in two groups of rats in: untreated and dexamethasone treated. The results showed a significant decline of GR protein immunopurified from untreated rats of advanced age. In dexamethasone-treated rats, the quantity of GR protein was lower than in controls at all ages. The interactions of liver nuclear proteins with radioactively labelled synthetic oligonucleotide analogue containing consensus GRE sequence were analysed during aging. The results showed that GRE binding activity demonstrated a decrease both in untreated and in dexamethasone treated rats. However, relative to untreated rats, dexamethasone treatment resulted in a significant increase in GRE binding at all ages, except that of three months old animals. In conclusion, the observed alterations in GR protein expression and its DNA binding activity may play a role in the changes of the cell response to glucocorticoid during aging. (c) 2007 Elsevier Inc. All rights reserved.
T2  - Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology
T1  - Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging
VL  - 148
IS  - 4
SP  - 463
EP  - 469
DO  - 10.1016/j.cbpb.2007.07.090
ER  - 

@article{
author = "Vujčić, Miroslava T. and Velickovic, Natasa and Ruždijić, Sabera",
year = "2007",
abstract = "Aging is associated with marked changes in the biochemical processes of many organs. Basal and glucocorticoid induced of liver nuclear glucocorticoid receptor (GR) on the level of protein expression and DNA-binding activity were investigated at different ages (3, 6, 12, 18 and 24 months old) in two groups of rats in: untreated and dexamethasone treated. The results showed a significant decline of GR protein immunopurified from untreated rats of advanced age. In dexamethasone-treated rats, the quantity of GR protein was lower than in controls at all ages. The interactions of liver nuclear proteins with radioactively labelled synthetic oligonucleotide analogue containing consensus GRE sequence were analysed during aging. The results showed that GRE binding activity demonstrated a decrease both in untreated and in dexamethasone treated rats. However, relative to untreated rats, dexamethasone treatment resulted in a significant increase in GRE binding at all ages, except that of three months old animals. In conclusion, the observed alterations in GR protein expression and its DNA binding activity may play a role in the changes of the cell response to glucocorticoid during aging. (c) 2007 Elsevier Inc. All rights reserved.",
journal = "Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology",
title = "Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging",
volume = "148",
number = "4",
pages = "463-469",
doi = "10.1016/j.cbpb.2007.07.090"
}

Vujčić, M. T., Velickovic, N.,& Ruždijić, S.. (2007). Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging. in Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology, 148(4), 463-469.
https://doi.org/10.1016/j.cbpb.2007.07.090

Vujčić MT, Velickovic N, Ruždijić S. Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging. in Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology. 2007;148(4):463-469.
doi:10.1016/j.cbpb.2007.07.090 .

Vujčić, Miroslava T., Velickovic, Natasa, Ruždijić, Sabera, "Dexamethasone treatment affects nuclear glucocorticoid receptor and glucocorticoid response element binding activity in liver of rats (Rattus norvegicus) during aging" in Comparative Biochemistry and Physiology. B: Biochemistry and Molecular Biology, 148, no. 4 (2007):463-469,
https://doi.org/10.1016/j.cbpb.2007.07.090 . .

TY  - JOUR
AU  - Vučić, Vesna
AU  - Isenović, Esma R.
AU  - Adžić, Miroslav
AU  - Ruždijić, Sabera
AU  - Radojčić, Marija
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2977
AB  - Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of Co-60 gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a significant (P LT 0.001) antiproliferative and cytotoxic effect on DU 145 cells. Both effects were time and dose dependent. Also, the dose of gamma-IR which inhibited DNA synthesis and cell proliferation by 50% was 9.7 Gy. Furthermore, gamma-IR induced cell cycle arrest in the G2/M phase and the percentage of cells in the G2/M phase was increased from 15% (control) to 49% (IR cells), with a nonsignificant induction of apoptosis. Treatment with 10 Gy gamma-IR for 24, 48, and 72 h stimulated CuZnSOD and MnSOD protein expression in a time-dependent manner, approximately by 3- to 3.5-fold. These data suggest that CuZnSOD and MnSOD enzymes may play an important role in the gamma-IR-induced changes in DU 145 cell growth, cell cycle arrest and cell death.
T2  - Brazilian Journal of Medical and Biological Research
T1  - Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells
VL  - 39
IS  - 2
SP  - 227
EP  - 236
DO  - 10.1590/S0100-879X2006000200009
ER  - 

@article{
author = "Vučić, Vesna and Isenović, Esma R. and Adžić, Miroslav and Ruždijić, Sabera and Radojčić, Marija",
year = "2006",
abstract = "Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of Co-60 gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a significant (P LT 0.001) antiproliferative and cytotoxic effect on DU 145 cells. Both effects were time and dose dependent. Also, the dose of gamma-IR which inhibited DNA synthesis and cell proliferation by 50% was 9.7 Gy. Furthermore, gamma-IR induced cell cycle arrest in the G2/M phase and the percentage of cells in the G2/M phase was increased from 15% (control) to 49% (IR cells), with a nonsignificant induction of apoptosis. Treatment with 10 Gy gamma-IR for 24, 48, and 72 h stimulated CuZnSOD and MnSOD protein expression in a time-dependent manner, approximately by 3- to 3.5-fold. These data suggest that CuZnSOD and MnSOD enzymes may play an important role in the gamma-IR-induced changes in DU 145 cell growth, cell cycle arrest and cell death.",
journal = "Brazilian Journal of Medical and Biological Research",
title = "Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells",
volume = "39",
number = "2",
pages = "227-236",
doi = "10.1590/S0100-879X2006000200009"
}

Vučić, V., Isenović, E. R., Adžić, M., Ruždijić, S.,& Radojčić, M.. (2006). Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells. in Brazilian Journal of Medical and Biological Research, 39(2), 227-236.
https://doi.org/10.1590/S0100-879X2006000200009

Vučić V, Isenović ER, Adžić M, Ruždijić S, Radojčić M. Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells. in Brazilian Journal of Medical and Biological Research. 2006;39(2):227-236.
doi:10.1590/S0100-879X2006000200009 .

Vučić, Vesna, Isenović, Esma R., Adžić, Miroslav, Ruždijić, Sabera, Radojčić, Marija, "Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells" in Brazilian Journal of Medical and Biological Research, 39, no. 2 (2006):227-236,
https://doi.org/10.1590/S0100-879X2006000200009 . .

TY  - CONF
AU  - Vučić, Vesna
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3203
C3  - European Journal of Cancer Supplements / EJC Supplements
T1  - Synergistic interaction between ionizing radiation and 8-chloro-adenosine 3 ,5 -monophosphate in PC-3 human prostate carcinoma cells
VL  - 3
IS  - 2
SP  - 258
EP  - 259
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3203
ER  - 

@conference{
author = "Vučić, Vesna and Radojčić, Marija and Ruždijić, Sabera",
year = "2005",
journal = "European Journal of Cancer Supplements / EJC Supplements",
title = "Synergistic interaction between ionizing radiation and 8-chloro-adenosine 3 ,5 -monophosphate in PC-3 human prostate carcinoma cells",
volume = "3",
number = "2",
pages = "258-259",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3203"
}

Vučić, V., Radojčić, M.,& Ruždijić, S.. (2005). Synergistic interaction between ionizing radiation and 8-chloro-adenosine 3 ,5 -monophosphate in PC-3 human prostate carcinoma cells. in European Journal of Cancer Supplements / EJC Supplements, 3(2), 258-259.
https://hdl.handle.net/21.15107/rcub_vinar_3203

Vučić V, Radojčić M, Ruždijić S. Synergistic interaction between ionizing radiation and 8-chloro-adenosine 3 ,5 -monophosphate in PC-3 human prostate carcinoma cells. in European Journal of Cancer Supplements / EJC Supplements. 2005;3(2):258-259.
https://hdl.handle.net/21.15107/rcub_vinar_3203 .

Vučić, Vesna, Radojčić, Marija, Ruždijić, Sabera, "Synergistic interaction between ionizing radiation and 8-chloro-adenosine 3 ,5 -monophosphate in PC-3 human prostate carcinoma cells" in European Journal of Cancer Supplements / EJC Supplements, 3, no. 2 (2005):258-259,
https://hdl.handle.net/21.15107/rcub_vinar_3203 .

TY  - JOUR
AU  - Vujčić, Miroslava T.
AU  - Terzić, N
AU  - Ristić-Fira, Aleksandra
AU  - Kanazir, Selma
AU  - Ruždijić, Sabera
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2906
AB  - In order to contribute to the understanding of mechanisms by which regulatory proteins recognize genetic information stored in DNA, analyses of their interaction with specific nucleotides are usually performed. In this study, the electrophoretic mobility shift assay (EMSA) was applied to analyze the interaction of nuclear proteins from the liver of rats of different age i.e., young (3-month-old), middle-aged (12-month-old) and aged (24-month-old), with radioactively labelled synthetic oligonucleotide analogues, corresponding to GRE. The levels of GRE binding activity were assessed by quantitative densitometric scanning of the autoradiograms. The results showed statistically significant decreasing values of up to 78% and 49% in middle aged and old animals, respectively, compared to young animals (p LT 0.05). The specificity of the nuclear proteins-GRE interaction was demonstrated by competition experiments with unlabelled GRE. In a supershift assay, using the antibody BuGR2, it was shown that the GR proteins present in nuclear extracts have a high affinity for the GRE probe. The stabilities of the protein-DNA complexes were analysed and it was concluded that they changed during ageing.
T2  - Journal of the Serbian Chemical Society
T1  - Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver
VL  - 70
IS  - 5
SP  - 705
EP  - 712
DO  - 10.2298/JSC0505705V
ER  - 

@article{
author = "Vujčić, Miroslava T. and Terzić, N and Ristić-Fira, Aleksandra and Kanazir, Selma and Ruždijić, Sabera",
year = "2005",
abstract = "In order to contribute to the understanding of mechanisms by which regulatory proteins recognize genetic information stored in DNA, analyses of their interaction with specific nucleotides are usually performed. In this study, the electrophoretic mobility shift assay (EMSA) was applied to analyze the interaction of nuclear proteins from the liver of rats of different age i.e., young (3-month-old), middle-aged (12-month-old) and aged (24-month-old), with radioactively labelled synthetic oligonucleotide analogues, corresponding to GRE. The levels of GRE binding activity were assessed by quantitative densitometric scanning of the autoradiograms. The results showed statistically significant decreasing values of up to 78% and 49% in middle aged and old animals, respectively, compared to young animals (p LT 0.05). The specificity of the nuclear proteins-GRE interaction was demonstrated by competition experiments with unlabelled GRE. In a supershift assay, using the antibody BuGR2, it was shown that the GR proteins present in nuclear extracts have a high affinity for the GRE probe. The stabilities of the protein-DNA complexes were analysed and it was concluded that they changed during ageing.",
journal = "Journal of the Serbian Chemical Society",
title = "Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver",
volume = "70",
number = "5",
pages = "705-712",
doi = "10.2298/JSC0505705V"
}

Vujčić, M. T., Terzić, N., Ristić-Fira, A., Kanazir, S.,& Ruždijić, S.. (2005). Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver. in Journal of the Serbian Chemical Society, 70(5), 705-712.
https://doi.org/10.2298/JSC0505705V

Vujčić MT, Terzić N, Ristić-Fira A, Kanazir S, Ruždijić S. Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver. in Journal of the Serbian Chemical Society. 2005;70(5):705-712.
doi:10.2298/JSC0505705V .

Vujčić, Miroslava T., Terzić, N, Ristić-Fira, Aleksandra, Kanazir, Selma, Ruždijić, Sabera, "Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver" in Journal of the Serbian Chemical Society, 70, no. 5 (2005):705-712,
https://doi.org/10.2298/JSC0505705V . .

TY  - CONF
AU  - Vučić, Vesna
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6488
AB  - Although radiotherapy and hormone therapy have been well established through a number of andomized studies, little is known about a possible interaction of both treatment modalities if they are given imultaneously. Radiotherapy and hormone therapy is combined in case of some types of breast cancer therapy. Estrogen signal is mediated within cells through specific binding to the estrogen receptor (ER).
The estrogen - ER protein complex binds with an increased affinity to a specific DNA sequence, called hormone response element (HRE), and consequently the transcription is initiated. ER expression appears to be generally low in normal breast epithelium, but it is over expressed in 60 - 70% of invasive
breast cancers. Cellular radiosensitivity and radiation induced DNA damage (double strand breaks) in both hormone sensitive and non-sensitive human breast cancer cell lines in presence or absence of estradiol were studied [Villalobos et al., Int. J. Radiat. Biol. 70(2), 161-169, 1996]. The findings of this study indicated that (1) sensitivity to radiation and the
proportion of proliferating cells are probably related, and (2) differences in radiosensitivity reflect differences in radiationinduced DNA damage.
The experimental study using plasmid DNA has been
performed in order to check how presence of estradiol
modifies initial yields of DNA damages induced by radiation. Method of agarose gel electrophoresis has been used to determine yields of particular types of DNA damages induced by g radiation of 6°Co. One or two HRE DNA base sequences were inserted into pCDNA3 plasmid. DNA plasmids were then irradiated in presence of increasing concentrations of estradiol and estradioI-ER protein. The yields of single and double strand breaks (SSB and DSB) determined as a function of absorbed dose and estradiol concentration in neutral and alkaline conditions will be presented and discussed. The radiomodifying properties of estradiol within cells will be estimated and compared to its effect on cell radiosensitivity due to induced grown-delay in hormone-sensitive cell lines.
C3  - Radiotherapy and Oncology
T1  - Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP
VL  - 73
SP  - S363
EP  - S363
DO  - 10.1016/S0167-8140(04)82718-X
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6488
ER  - 

@conference{
author = "Vučić, Vesna and Radojčić, Marija and Ruždijić, Sabera",
year = "2004",
abstract = "Although radiotherapy and hormone therapy have been well established through a number of andomized studies, little is known about a possible interaction of both treatment modalities if they are given imultaneously. Radiotherapy and hormone therapy is combined in case of some types of breast cancer therapy. Estrogen signal is mediated within cells through specific binding to the estrogen receptor (ER).
The estrogen - ER protein complex binds with an increased affinity to a specific DNA sequence, called hormone response element (HRE), and consequently the transcription is initiated. ER expression appears to be generally low in normal breast epithelium, but it is over expressed in 60 - 70% of invasive
breast cancers. Cellular radiosensitivity and radiation induced DNA damage (double strand breaks) in both hormone sensitive and non-sensitive human breast cancer cell lines in presence or absence of estradiol were studied [Villalobos et al., Int. J. Radiat. Biol. 70(2), 161-169, 1996]. The findings of this study indicated that (1) sensitivity to radiation and the
proportion of proliferating cells are probably related, and (2) differences in radiosensitivity reflect differences in radiationinduced DNA damage.
The experimental study using plasmid DNA has been
performed in order to check how presence of estradiol
modifies initial yields of DNA damages induced by radiation. Method of agarose gel electrophoresis has been used to determine yields of particular types of DNA damages induced by g radiation of 6°Co. One or two HRE DNA base sequences were inserted into pCDNA3 plasmid. DNA plasmids were then irradiated in presence of increasing concentrations of estradiol and estradioI-ER protein. The yields of single and double strand breaks (SSB and DSB) determined as a function of absorbed dose and estradiol concentration in neutral and alkaline conditions will be presented and discussed. The radiomodifying properties of estradiol within cells will be estimated and compared to its effect on cell radiosensitivity due to induced grown-delay in hormone-sensitive cell lines.",
journal = "Radiotherapy and Oncology",
title = "Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP",
volume = "73",
pages = "S363-S363",
doi = "10.1016/S0167-8140(04)82718-X",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6488"
}

Vučić, V., Radojčić, M.,& Ruždijić, S.. (2004). Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP. in Radiotherapy and Oncology, 73, S363-S363.
https://doi.org/10.1016/S0167-8140(04)82718-X
https://hdl.handle.net/21.15107/rcub_vinar_6488

Vučić V, Radojčić M, Ruždijić S. Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP. in Radiotherapy and Oncology. 2004;73:S363-S363.
doi:10.1016/S0167-8140(04)82718-X
https://hdl.handle.net/21.15107/rcub_vinar_6488 .

Vučić, Vesna, Radojčić, Marija, Ruždijić, Sabera, "Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP" in Radiotherapy and Oncology, 73 (2004):S363-S363,
https://doi.org/10.1016/S0167-8140(04)82718-X .,
https://hdl.handle.net/21.15107/rcub_vinar_6488 .

TY  - JOUR
AU  - Korićanac, Lela
AU  - Todorović, Danijela V.
AU  - Popović, Nataša M.
AU  - Demajo, Miroslav
AU  - Ruždijić, Sabera
AU  - Ristić-Fira, Aleksandra
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6500
AB  - Novel antineoplastic agents, 8-chloroadenosine 3,5-monophosphate (8-Cl-cAMP) and tiazofurin (TR), have been shown to be effective against different malignant cells. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, and apoptosis. The aim of this work was the in vitro study of either 8-Cl-cAMP or TR effects on B16/F10 and B16/C3 mouse melanoma cell growth and cell death. Significant cell growth inhibition was obtained after the application of 8-Cl-cAMP or TR. The presence and number of apoptotic cells was evaluated using agarose gel electrophoresis and flow cytometry. The number of apoptotic nuclei, after treatment with antineoplastic agents, did not significantly change in B16/F10 cells, although it did show a significant increase in B16/C3 cells. The expression of c-myc did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. The same results were obtained in B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression showed a significant increase in B16/C3 cells after treatment with TR. Concerning the effects that the analyzed agents exhibited on melanoma cells and other cancer cells, further preclinical studies of these drugs will potentially lead to better understanding of the molecular mechanisms of their action and finally more efficient therapeutic approaches to malignant diseases.
T2  - Annals of the New York Academy of Sciences
T1  - Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin
VL  - 1030
SP  - 384
EP  - 392
DO  - 10.1196/annals.1329.048
ER  - 

@article{
author = "Korićanac, Lela and Todorović, Danijela V. and Popović, Nataša M. and Demajo, Miroslav and Ruždijić, Sabera and Ristić-Fira, Aleksandra",
year = "2004",
abstract = "Novel antineoplastic agents, 8-chloroadenosine 3,5-monophosphate (8-Cl-cAMP) and tiazofurin (TR), have been shown to be effective against different malignant cells. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, and apoptosis. The aim of this work was the in vitro study of either 8-Cl-cAMP or TR effects on B16/F10 and B16/C3 mouse melanoma cell growth and cell death. Significant cell growth inhibition was obtained after the application of 8-Cl-cAMP or TR. The presence and number of apoptotic cells was evaluated using agarose gel electrophoresis and flow cytometry. The number of apoptotic nuclei, after treatment with antineoplastic agents, did not significantly change in B16/F10 cells, although it did show a significant increase in B16/C3 cells. The expression of c-myc did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. The same results were obtained in B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression showed a significant increase in B16/C3 cells after treatment with TR. Concerning the effects that the analyzed agents exhibited on melanoma cells and other cancer cells, further preclinical studies of these drugs will potentially lead to better understanding of the molecular mechanisms of their action and finally more efficient therapeutic approaches to malignant diseases.",
journal = "Annals of the New York Academy of Sciences",
title = "Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin",
volume = "1030",
pages = "384-392",
doi = "10.1196/annals.1329.048"
}

Korićanac, L., Todorović, D. V., Popović, N. M., Demajo, M., Ruždijić, S.,& Ristić-Fira, A.. (2004). Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin. in Annals of the New York Academy of Sciences, 1030, 384-392.
https://doi.org/10.1196/annals.1329.048

Korićanac L, Todorović DV, Popović NM, Demajo M, Ruždijić S, Ristić-Fira A. Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin. in Annals of the New York Academy of Sciences. 2004;1030:384-392.
doi:10.1196/annals.1329.048 .

Korićanac, Lela, Todorović, Danijela V., Popović, Nataša M., Demajo, Miroslav, Ruždijić, Sabera, Ristić-Fira, Aleksandra, "Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin" in Annals of the New York Academy of Sciences, 1030 (2004):384-392,
https://doi.org/10.1196/annals.1329.048 . .

TY  - CONF
AU  - Vučić, Vesna
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2727
C3  - Annals of Oncology
T1  - Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP
VL  - 15
SP  - 115
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2727
ER  - 

@conference{
author = "Vučić, Vesna and Radojčić, Marija and Ruždijić, Sabera",
year = "2004",
journal = "Annals of Oncology",
title = "Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP",
volume = "15",
pages = "115-115",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2727"
}

Vučić, V., Radojčić, M.,& Ruždijić, S.. (2004). Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP. in Annals of Oncology, 15, 115-115.
https://hdl.handle.net/21.15107/rcub_vinar_2727

Vučić V, Radojčić M, Ruždijić S. Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP. in Annals of Oncology. 2004;15:115-115.
https://hdl.handle.net/21.15107/rcub_vinar_2727 .

Vučić, Vesna, Radojčić, Marija, Ruždijić, Sabera, "Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP" in Annals of Oncology, 15 (2004):115-115,
https://hdl.handle.net/21.15107/rcub_vinar_2727 .

TY  - JOUR
AU  - Vučić, Vesna
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Tišma, Nevena
AU  - Ruždijić, Sabera
AU  - Radojčić, Marija B.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10583
AB  - Despite the significant advances in cancer chemotherapy, radiotherapy still remains a method of choice for treatment of metastatic human prostate cancer. This study presents quantitative analysis of 60Co gamma-radiation effects on cell growth and cell death of metastatic human prostate cancer PC-3 cell line, performed in time (24-72h) and dose (2-20 Gy) dependent manner. The irradiated PC-3 cells were mostly dying by necrosis at late time intervals (72h), while apoptotic cell death was negligible. The EC50 or 50% of cytotoxicity was not achieved within the radiation doses used (2-20 Gy), but significant cell growth inhibition with IC50 of 10.4 Gy was observed. It is concluded that the increase in the radiation dose may have an important cytostatic effect, but for the complete eradication of metastatic prostate cancer novel cytotoxic drugs and radiosensitizers should be introduced as adjuvant.
T2  - Jugoslovenska medicinska biohemija
T1  - Cell death in irradiated prostate cancer cells assessed by flow cytometry
VL  - 23
IS  - 4
SP  - 343
EP  - 350
DO  - 10.2298/JMH0404343V
ER  - 

@article{
author = "Vučić, Vesna and Adžić, Miroslav and Nićiforović, Ana and Tišma, Nevena and Ruždijić, Sabera and Radojčić, Marija B.",
year = "2004",
abstract = "Despite the significant advances in cancer chemotherapy, radiotherapy still remains a method of choice for treatment of metastatic human prostate cancer. This study presents quantitative analysis of 60Co gamma-radiation effects on cell growth and cell death of metastatic human prostate cancer PC-3 cell line, performed in time (24-72h) and dose (2-20 Gy) dependent manner. The irradiated PC-3 cells were mostly dying by necrosis at late time intervals (72h), while apoptotic cell death was negligible. The EC50 or 50% of cytotoxicity was not achieved within the radiation doses used (2-20 Gy), but significant cell growth inhibition with IC50 of 10.4 Gy was observed. It is concluded that the increase in the radiation dose may have an important cytostatic effect, but for the complete eradication of metastatic prostate cancer novel cytotoxic drugs and radiosensitizers should be introduced as adjuvant.",
journal = "Jugoslovenska medicinska biohemija",
title = "Cell death in irradiated prostate cancer cells assessed by flow cytometry",
volume = "23",
number = "4",
pages = "343-350",
doi = "10.2298/JMH0404343V"
}

Vučić, V., Adžić, M., Nićiforović, A., Tišma, N., Ruždijić, S.,& Radojčić, M. B.. (2004). Cell death in irradiated prostate cancer cells assessed by flow cytometry. in Jugoslovenska medicinska biohemija, 23(4), 343-350.
https://doi.org/10.2298/JMH0404343V

Vučić V, Adžić M, Nićiforović A, Tišma N, Ruždijić S, Radojčić MB. Cell death in irradiated prostate cancer cells assessed by flow cytometry. in Jugoslovenska medicinska biohemija. 2004;23(4):343-350.
doi:10.2298/JMH0404343V .

Vučić, Vesna, Adžić, Miroslav, Nićiforović, Ana, Tišma, Nevena, Ruždijić, Sabera, Radojčić, Marija B., "Cell death in irradiated prostate cancer cells assessed by flow cytometry" in Jugoslovenska medicinska biohemija, 23, no. 4 (2004):343-350,
https://doi.org/10.2298/JMH0404343V . .

TY  - CONF
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Vujčić, Miroslava T.
AU  - Korićanac, Lela
AU  - Ruždijić, Sabera
AU  - Demajo, Miroslav
AU  - Cuttone, Giacomo
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8631
C3  - Turkish Journal of Biochemistry
T1  - Inactivation of melanoma cells irradiated with gamma rays and low energy protons
VL  - 28
IS  - 3
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8631
ER  - 

@conference{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Vujčić, Miroslava T. and Korićanac, Lela and Ruždijić, Sabera and Demajo, Miroslav and Cuttone, Giacomo",
year = "2003",
journal = "Turkish Journal of Biochemistry",
title = "Inactivation of melanoma cells irradiated with gamma rays and low energy protons",
volume = "28",
number = "3",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8631"
}

Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Vujčić, M. T., Korićanac, L., Ruždijić, S., Demajo, M.,& Cuttone, G.. (2003). Inactivation of melanoma cells irradiated with gamma rays and low energy protons. in Turkish Journal of Biochemistry, 28(3), 85.
https://hdl.handle.net/21.15107/rcub_vinar_8631

Petrović IM, Ristić-Fira A, Todorović DV, Vujčić MT, Korićanac L, Ruždijić S, Demajo M, Cuttone G. Inactivation of melanoma cells irradiated with gamma rays and low energy protons. in Turkish Journal of Biochemistry. 2003;28(3):85.
https://hdl.handle.net/21.15107/rcub_vinar_8631 .

Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Vujčić, Miroslava T., Korićanac, Lela, Ruždijić, Sabera, Demajo, Miroslav, Cuttone, Giacomo, "Inactivation of melanoma cells irradiated with gamma rays and low energy protons" in Turkish Journal of Biochemistry, 28, no. 3 (2003):85,
https://hdl.handle.net/21.15107/rcub_vinar_8631 .

TY  - CONF
AU  - Vučić, Vesna
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Tišma, Nevena
AU  - Janković, Dragana
AU  - Ruždijić, Sabera
AU  - Radojčić, Marija B.
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12038
AB  - Background: Prostate cancer is the first as an incidence and the second as a cause of the oncologic mortality in the male population. There is a broad range of possibilities in the prostate cancer therapy. However, there is also much controversy on the most appropriate treatment in the various stages of the disease. Advanced disease is mostly treated by radiation therapy, sometimes in combination with hormone or chemotherapy. Irradiation induces damage of cell biomolecules, which can lead to the arrest in cell division, or to apoptotic or necrotic cell death. The aim of this study was to determine the dose dependence of radiation-induced cell death in two human prostate cancer cell lines, and to define the form of death of these cells. Methods: Human prostate cancer cell lines PC-3 and DU-145 were irradiated with 2 - 30 Gy from 60 Co g-source, at the dose rate of 20 Gy/h. The effect of irradiation on cell viability, morphology and DNA structure were followed 24 - 72 hours after treatment. Cells were analyzed by trypan blue exclusion assay, flow cytometry and DNA gel electrophoresis. Simultaneous staining of cells with Annexin V-FITC and propidium iodide enabled distinction of early apoptosis from late apoptosis and/or necrosis. Results: The results of trypan blue staining indicated that radiation-induced cell death was both time and dose dependent process. According to flow-cytometry and DNA fragmentation assay, necrosis was the prevailing form of the radiation-induced cell death in both PC-3 and DU-145 cells. The apoptosis occurred in insignificant number of cells, probably due to the mutant p53 gene present in both cell lines. The cell necrosis was dose dependent and was most pronounced 72 hours post treatment. Conclusion The prevailing form of radiation-induced PC-3 and DU-145 cell death is necrosis. Both PC-3 and DU-145 are rather radioresistant cell lines, as the dose necessary to induce 50% decrease in viable cell number is about 10 Gy.
C3  - Archive of Oncology
T1  - Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells
VL  - 11
IS  - 3
SP  - 197
EP  - 197
DO  - 10.2298/AOO0303197V
ER  - 

@conference{
author = "Vučić, Vesna and Nićiforović, Ana and Adžić, Miroslav and Tišma, Nevena and Janković, Dragana and Ruždijić, Sabera and Radojčić, Marija B.",
year = "2003",
abstract = "Background: Prostate cancer is the first as an incidence and the second as a cause of the oncologic mortality in the male population. There is a broad range of possibilities in the prostate cancer therapy. However, there is also much controversy on the most appropriate treatment in the various stages of the disease. Advanced disease is mostly treated by radiation therapy, sometimes in combination with hormone or chemotherapy. Irradiation induces damage of cell biomolecules, which can lead to the arrest in cell division, or to apoptotic or necrotic cell death. The aim of this study was to determine the dose dependence of radiation-induced cell death in two human prostate cancer cell lines, and to define the form of death of these cells. Methods: Human prostate cancer cell lines PC-3 and DU-145 were irradiated with 2 - 30 Gy from 60 Co g-source, at the dose rate of 20 Gy/h. The effect of irradiation on cell viability, morphology and DNA structure were followed 24 - 72 hours after treatment. Cells were analyzed by trypan blue exclusion assay, flow cytometry and DNA gel electrophoresis. Simultaneous staining of cells with Annexin V-FITC and propidium iodide enabled distinction of early apoptosis from late apoptosis and/or necrosis. Results: The results of trypan blue staining indicated that radiation-induced cell death was both time and dose dependent process. According to flow-cytometry and DNA fragmentation assay, necrosis was the prevailing form of the radiation-induced cell death in both PC-3 and DU-145 cells. The apoptosis occurred in insignificant number of cells, probably due to the mutant p53 gene present in both cell lines. The cell necrosis was dose dependent and was most pronounced 72 hours post treatment. Conclusion The prevailing form of radiation-induced PC-3 and DU-145 cell death is necrosis. Both PC-3 and DU-145 are rather radioresistant cell lines, as the dose necessary to induce 50% decrease in viable cell number is about 10 Gy.",
journal = "Archive of Oncology",
title = "Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells",
volume = "11",
number = "3",
pages = "197-197",
doi = "10.2298/AOO0303197V"
}

Vučić, V., Nićiforović, A., Adžić, M., Tišma, N., Janković, D., Ruždijić, S.,& Radojčić, M. B.. (2003). Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells. in Archive of Oncology, 11(3), 197-197.
https://doi.org/10.2298/AOO0303197V

Vučić V, Nićiforović A, Adžić M, Tišma N, Janković D, Ruždijić S, Radojčić MB. Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells. in Archive of Oncology. 2003;11(3):197-197.
doi:10.2298/AOO0303197V .

Vučić, Vesna, Nićiforović, Ana, Adžić, Miroslav, Tišma, Nevena, Janković, Dragana, Ruždijić, Sabera, Radojčić, Marija B., "Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells" in Archive of Oncology, 11, no. 3 (2003):197-197,
https://doi.org/10.2298/AOO0303197V . .

TY  - JOUR
AU  - Terzić, N
AU  - Vujčić, Miroslava T.
AU  - Ristić-Fira, Aleksandra
AU  - Krstić-Demonacos, Marija
AU  - Milanovic, D
AU  - Kanazir, Dušan T.
AU  - Ruždijić, Sabera
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2623
AB  - The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX treatment in an age-specific manner.
T2  - Journals of Gerontology. Series A: Biological Sciences and Medical Sciences
T1  - Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain
VL  - 58
IS  - 4
SP  - 297
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2623
ER  - 

@article{
author = "Terzić, N and Vujčić, Miroslava T. and Ristić-Fira, Aleksandra and Krstić-Demonacos, Marija and Milanovic, D and Kanazir, Dušan T. and Ruždijić, Sabera",
year = "2003",
abstract = "The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-1) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein-protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-I regulatory elements changes upon DEX treatment in an age-specific manner.",
journal = "Journals of Gerontology. Series A: Biological Sciences and Medical Sciences",
title = "Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain",
volume = "58",
number = "4",
pages = "297-303",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2623"
}

Terzić, N., Vujčić, M. T., Ristić-Fira, A., Krstić-Demonacos, M., Milanovic, D., Kanazir, D. T.,& Ruždijić, S.. (2003). Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain. in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 58(4), 297-303.
https://hdl.handle.net/21.15107/rcub_vinar_2623

Terzić N, Vujčić MT, Ristić-Fira A, Krstić-Demonacos M, Milanovic D, Kanazir DT, Ruždijić S. Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain. in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences. 2003;58(4):297-303.
https://hdl.handle.net/21.15107/rcub_vinar_2623 .

Terzić, N, Vujčić, Miroslava T., Ristić-Fira, Aleksandra, Krstić-Demonacos, Marija, Milanovic, D, Kanazir, Dušan T., Ruždijić, Sabera, "Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain" in Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 58, no. 4 (2003):297-303,
https://hdl.handle.net/21.15107/rcub_vinar_2623 .

TY  - JOUR
AU  - Ruždijić, Sabera
AU  - Milošević, Jovan
AU  - Popović, Nataša M.
AU  - Pešić, Milica
AU  - Stojilkovic, M
AU  - Kanazir, Selma
AU  - Todorović, Danijela V.
AU  - Ristić-Fira, Aleksandra
AU  - Krstić-Demonacos, Marija
AU  - Kanazir, Selma
AU  - Rakić, Ljubisav
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2423
AB  - Tiazofurin and 8-Cl-cAMP are novel antineoplastic agents that have been shown to be effective against various cancer cells in vitro and in vivo. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, apoptosis and downregulation of c-ras and c-myc gene expression. We examined the effects of 8-Cl-cAMP and tiazofurin, either separately or together, on apoptosis induction and c-fos and c-myc expression in melanoma cells. 8-Cl-cAMP and tiazofurin inhibited the growth of melanoma cells in a dose-responsive manner. Whether used separately or together, each agent induced apoptotic cell death. Apoptosis was accompanied by a marked inhibition of c-fos and c-mye gene expression. RT-PCR analysis showed that 8-Cl-cAMP, together with tiazofurin, promoted 61% and 75% decreases of c-myc and c-fos expression in melanoma cells respectively. These results clearly indicate that the combination of 8-Cl-cAMP and tiazofurin could provide a promising therapeutic approach for melanoma treatment.
T2  - Jugoslovenska Medicinska Biohemija
T1  - Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells
VL  - 20
IS  - 1
SP  - 9
EP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2423
ER  - 

@article{
author = "Ruždijić, Sabera and Milošević, Jovan and Popović, Nataša M. and Pešić, Milica and Stojilkovic, M and Kanazir, Selma and Todorović, Danijela V. and Ristić-Fira, Aleksandra and Krstić-Demonacos, Marija and Kanazir, Selma and Rakić, Ljubisav",
year = "2001",
abstract = "Tiazofurin and 8-Cl-cAMP are novel antineoplastic agents that have been shown to be effective against various cancer cells in vitro and in vivo. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, apoptosis and downregulation of c-ras and c-myc gene expression. We examined the effects of 8-Cl-cAMP and tiazofurin, either separately or together, on apoptosis induction and c-fos and c-myc expression in melanoma cells. 8-Cl-cAMP and tiazofurin inhibited the growth of melanoma cells in a dose-responsive manner. Whether used separately or together, each agent induced apoptotic cell death. Apoptosis was accompanied by a marked inhibition of c-fos and c-mye gene expression. RT-PCR analysis showed that 8-Cl-cAMP, together with tiazofurin, promoted 61% and 75% decreases of c-myc and c-fos expression in melanoma cells respectively. These results clearly indicate that the combination of 8-Cl-cAMP and tiazofurin could provide a promising therapeutic approach for melanoma treatment.",
journal = "Jugoslovenska Medicinska Biohemija",
title = "Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells",
volume = "20",
number = "1",
pages = "9-18",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2423"
}

Ruždijić, S., Milošević, J., Popović, N. M., Pešić, M., Stojilkovic, M., Kanazir, S., Todorović, D. V., Ristić-Fira, A., Krstić-Demonacos, M., Kanazir, S.,& Rakić, L.. (2001). Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells. in Jugoslovenska Medicinska Biohemija, 20(1), 9-18.
https://hdl.handle.net/21.15107/rcub_vinar_2423

Ruždijić S, Milošević J, Popović NM, Pešić M, Stojilkovic M, Kanazir S, Todorović DV, Ristić-Fira A, Krstić-Demonacos M, Kanazir S, Rakić L. Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells. in Jugoslovenska Medicinska Biohemija. 2001;20(1):9-18.
https://hdl.handle.net/21.15107/rcub_vinar_2423 .

Ruždijić, Sabera, Milošević, Jovan, Popović, Nataša M., Pešić, Milica, Stojilkovic, M, Kanazir, Selma, Todorović, Danijela V., Ristić-Fira, Aleksandra, Krstić-Demonacos, Marija, Kanazir, Selma, Rakić, Ljubisav, "Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells" in Jugoslovenska Medicinska Biohemija, 20, no. 1 (2001):9-18,
https://hdl.handle.net/21.15107/rcub_vinar_2423 .

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Nikolic, D
AU  - Petrović, Ivan M.
AU  - Ruždijić, Sabera
AU  - Raffaele, L
AU  - Sabini, MG
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Farruggia, G
AU  - Masotti, L
AU  - Kanazir, Dušan T.
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2426
AB  - The aim of this work is the in vitro study of the late effects of single proton irradiation on HTB63 human melanoma cell growth, cell cycle and cell death. The experimental conditions were focused on analyzing the effects of irradiation on the periphery of tumour that can be, in clinical practice, close to critical organs. Confluent cell monolayers were irradiated with single doses ranging from 1 - 20 Gy, using proton beams having an energy of 22.6 MeV at the target. Antiproliferative effect of protons, cell cycle analysis and initiation of cell death, were followed 48 hours after irradiation. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analysis and cell viability have shown the G2/M and G1/G0 arrest of irradiated cells correlating with the increase of the applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. These data demonstrate that irradiation with protons, under the chosen experimental conditions, have significant effects on melanoma cell growth inhibition being dose dependent, G2/M cell cycle arrest and appearance of apoptotic nuclei, even 48 hours after irradiation. The results obtained may help the understanding of the relationship between cell proliferation, death and cell cycle regulation of melanomas after proton irradiation.
T2  - Journal of Experimental and Clinical Cancer Research
T1  - The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line
VL  - 20
IS  - 1
SP  - 135
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2426
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Nikolic, D and Petrović, Ivan M. and Ruždijić, Sabera and Raffaele, L and Sabini, MG and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Farruggia, G and Masotti, L and Kanazir, Dušan T.",
year = "2001",
abstract = "The aim of this work is the in vitro study of the late effects of single proton irradiation on HTB63 human melanoma cell growth, cell cycle and cell death. The experimental conditions were focused on analyzing the effects of irradiation on the periphery of tumour that can be, in clinical practice, close to critical organs. Confluent cell monolayers were irradiated with single doses ranging from 1 - 20 Gy, using proton beams having an energy of 22.6 MeV at the target. Antiproliferative effect of protons, cell cycle analysis and initiation of cell death, were followed 48 hours after irradiation. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analysis and cell viability have shown the G2/M and G1/G0 arrest of irradiated cells correlating with the increase of the applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. These data demonstrate that irradiation with protons, under the chosen experimental conditions, have significant effects on melanoma cell growth inhibition being dose dependent, G2/M cell cycle arrest and appearance of apoptotic nuclei, even 48 hours after irradiation. The results obtained may help the understanding of the relationship between cell proliferation, death and cell cycle regulation of melanomas after proton irradiation.",
journal = "Journal of Experimental and Clinical Cancer Research",
title = "The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line",
volume = "20",
number = "1",
pages = "135-143",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2426"
}

Ristić-Fira, A., Nikolic, D., Petrović, I. M., Ruždijić, S., Raffaele, L., Sabini, M., Cirrone, G. A. P., Cuttone, G., Farruggia, G., Masotti, L.,& Kanazir, D. T.. (2001). The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line. in Journal of Experimental and Clinical Cancer Research, 20(1), 135-143.
https://hdl.handle.net/21.15107/rcub_vinar_2426

Ristić-Fira A, Nikolic D, Petrović IM, Ruždijić S, Raffaele L, Sabini M, Cirrone GAP, Cuttone G, Farruggia G, Masotti L, Kanazir DT. The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line. in Journal of Experimental and Clinical Cancer Research. 2001;20(1):135-143.
https://hdl.handle.net/21.15107/rcub_vinar_2426 .

Ristić-Fira, Aleksandra, Nikolic, D, Petrović, Ivan M., Ruždijić, Sabera, Raffaele, L, Sabini, MG, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Farruggia, G, Masotti, L, Kanazir, Dušan T., "The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line" in Journal of Experimental and Clinical Cancer Research, 20, no. 1 (2001):135-143,
https://hdl.handle.net/21.15107/rcub_vinar_2426 .

TY  - JOUR
AU  - Djordjevic-Markovic, R
AU  - Radić, Olivera
AU  - Jelic, V
AU  - Radojčić, Marija
AU  - Rapic-Otrin, V
AU  - Ruždijić, Sabera
AU  - Krstić-Demonacos, Marija
AU  - Kanazir, Selma
AU  - Kanazir, Selma
PY  - 1999
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2305
AB  - The role of the glucocorticoid receptor (GR) in senescence was studied in rats of increasing age. Statistically significant changes in the number of GRs from rat liver were detected, whereas the affinity for the ligand triamcinolone acetonide (TA) did not change with increasing age, and was in the range of 1-2 nM. In all cases the number of receptors was lower in rats treated with hormone in vivo relative to untreated animals. In addition, we have found changes in GR activation, as measured by the binding to DNA cellulose in the mentioned age groups. Furthermore, expression of the glucocorticoid hormone (GH)-inducible gene, tyrosine amino transferase (TAT) also showed age-related alterations. We conclude that receptor function shows oscillatory changes during ageing. In addition, response to GH generally declines towards the older age. This. specific periodicity in functional characteristics of the GR may reconcile conflicting results about the receptor number and properties during the ageing process, and marks particular age at which individual organism shows the highest or the lowest sensitivity to the actions of GH. (C) 1999 Elsevier Science Inc. All rights reserved.
T2  - Experimental Gerontology
T1  - Glucocorticoid receptors in ageing rats
VL  - 34
IS  - 8
SP  - 971
EP  - 982
DO  - 10.1016/S0531-5565(99)00067-4
ER  - 

@article{
author = "Djordjevic-Markovic, R and Radić, Olivera and Jelic, V and Radojčić, Marija and Rapic-Otrin, V and Ruždijić, Sabera and Krstić-Demonacos, Marija and Kanazir, Selma and Kanazir, Selma",
year = "1999",
abstract = "The role of the glucocorticoid receptor (GR) in senescence was studied in rats of increasing age. Statistically significant changes in the number of GRs from rat liver were detected, whereas the affinity for the ligand triamcinolone acetonide (TA) did not change with increasing age, and was in the range of 1-2 nM. In all cases the number of receptors was lower in rats treated with hormone in vivo relative to untreated animals. In addition, we have found changes in GR activation, as measured by the binding to DNA cellulose in the mentioned age groups. Furthermore, expression of the glucocorticoid hormone (GH)-inducible gene, tyrosine amino transferase (TAT) also showed age-related alterations. We conclude that receptor function shows oscillatory changes during ageing. In addition, response to GH generally declines towards the older age. This. specific periodicity in functional characteristics of the GR may reconcile conflicting results about the receptor number and properties during the ageing process, and marks particular age at which individual organism shows the highest or the lowest sensitivity to the actions of GH. (C) 1999 Elsevier Science Inc. All rights reserved.",
journal = "Experimental Gerontology",
title = "Glucocorticoid receptors in ageing rats",
volume = "34",
number = "8",
pages = "971-982",
doi = "10.1016/S0531-5565(99)00067-4"
}

Djordjevic-Markovic, R., Radić, O., Jelic, V., Radojčić, M., Rapic-Otrin, V., Ruždijić, S., Krstić-Demonacos, M., Kanazir, S.,& Kanazir, S.. (1999). Glucocorticoid receptors in ageing rats. in Experimental Gerontology, 34(8), 971-982.
https://doi.org/10.1016/S0531-5565(99)00067-4

Djordjevic-Markovic R, Radić O, Jelic V, Radojčić M, Rapic-Otrin V, Ruždijić S, Krstić-Demonacos M, Kanazir S, Kanazir S. Glucocorticoid receptors in ageing rats. in Experimental Gerontology. 1999;34(8):971-982.
doi:10.1016/S0531-5565(99)00067-4 .

Djordjevic-Markovic, R, Radić, Olivera, Jelic, V, Radojčić, Marija, Rapic-Otrin, V, Ruždijić, Sabera, Krstić-Demonacos, Marija, Kanazir, Selma, Kanazir, Selma, "Glucocorticoid receptors in ageing rats" in Experimental Gerontology, 34, no. 8 (1999):971-982,
https://doi.org/10.1016/S0531-5565(99)00067-4 . .

TY  - JOUR
AU  - Počuča, Nataša
AU  - Ruždijić, Sabera
AU  - Demonacos, Constantinos
AU  - Kanazir, Dušan T.
AU  - Krstić-Demonacos, Marija
PY  - 1998
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2188
AB  - The glucocorticoid receptor (GR) is a phosphoprotein and a member of the steroid/thyroid receptor superfamily of ligand dependent transcription factors. When the glucocorticoid receptor is expressed in yeast (Saccharomyces cerevisiae), it is competent for signal transduction and transcriptional regulation. We have studied the glucocorticoid receptor phosphorylation in yeast and demonstrated that the receptor is phosphorylated in both the absence and presence of hormone, on serine and threonine residues. This phosphorylation occurs within 15 min upon addition of radioactivity in both hormone treated and untreated cells. As reported for mammalian cells, additional phosphorylation occurs upon hormone binding and this phosphorylation is dependent on the type of the ligand. We have followed the hormone dependent receptor phosphorylation by electrophoretic mobility shift assay, and have shown that this mobility change is sensitive to phosphatase treatment. In addition, the appearance of hormone dependent phosphoisoforms of the receptor depends on the potency of the agonist used. Using this method we show that the residues contributing to the hormone dependent mobility shift are localized in one of the transcriptional activation domains, between amino acids 130-247. We altered the phosphorylation sites within this domain that correspond to the amino acids phosphorylated in mouse hormone treated cells. Using phosphopeptide maps we show that hormone changes the peptide pattern of metabolically labelled receptor, and we identify peptides which are phosphorylated in hormone dependent manner. Then we determine that phosphorylation of residues S224 and S232 is increased in the presence of hormone, whereas phosphorylation of residues T171 and S246 is constitutive. Finally, we show that in both yeast and mammalian cells the same residues on the glucocorticoid receptor are phosphorylated. Our results suggest that yeast cells would be a suitable system to study glucocorticoid receptor phosphorylation. The genetic manipulability of yeast cells, together with conservation of the phosphorylation of GR in yeast and mammalian cells and identification of hormone dependent phosphorylation, would facilitate the isolation of molecules involved in the glucocorticoid receptor phosphorylation pathway and further our understanding of this process. (C) 1998 Elsevier Science Ltd. All rights reserved.
T2  - Journal of Steroid Biochemistry and Molecular Biology
T1  - Using yeast to study glucocorticoid receptor phosphorylation
VL  - 66
IS  - 5-6
SP  - 303
EP  - 318
DO  - 10.1016/S0960-0760(98)00057-0
ER  - 

@article{
author = "Počuča, Nataša and Ruždijić, Sabera and Demonacos, Constantinos and Kanazir, Dušan T. and Krstić-Demonacos, Marija",
year = "1998",
abstract = "The glucocorticoid receptor (GR) is a phosphoprotein and a member of the steroid/thyroid receptor superfamily of ligand dependent transcription factors. When the glucocorticoid receptor is expressed in yeast (Saccharomyces cerevisiae), it is competent for signal transduction and transcriptional regulation. We have studied the glucocorticoid receptor phosphorylation in yeast and demonstrated that the receptor is phosphorylated in both the absence and presence of hormone, on serine and threonine residues. This phosphorylation occurs within 15 min upon addition of radioactivity in both hormone treated and untreated cells. As reported for mammalian cells, additional phosphorylation occurs upon hormone binding and this phosphorylation is dependent on the type of the ligand. We have followed the hormone dependent receptor phosphorylation by electrophoretic mobility shift assay, and have shown that this mobility change is sensitive to phosphatase treatment. In addition, the appearance of hormone dependent phosphoisoforms of the receptor depends on the potency of the agonist used. Using this method we show that the residues contributing to the hormone dependent mobility shift are localized in one of the transcriptional activation domains, between amino acids 130-247. We altered the phosphorylation sites within this domain that correspond to the amino acids phosphorylated in mouse hormone treated cells. Using phosphopeptide maps we show that hormone changes the peptide pattern of metabolically labelled receptor, and we identify peptides which are phosphorylated in hormone dependent manner. Then we determine that phosphorylation of residues S224 and S232 is increased in the presence of hormone, whereas phosphorylation of residues T171 and S246 is constitutive. Finally, we show that in both yeast and mammalian cells the same residues on the glucocorticoid receptor are phosphorylated. Our results suggest that yeast cells would be a suitable system to study glucocorticoid receptor phosphorylation. The genetic manipulability of yeast cells, together with conservation of the phosphorylation of GR in yeast and mammalian cells and identification of hormone dependent phosphorylation, would facilitate the isolation of molecules involved in the glucocorticoid receptor phosphorylation pathway and further our understanding of this process. (C) 1998 Elsevier Science Ltd. All rights reserved.",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
title = "Using yeast to study glucocorticoid receptor phosphorylation",
volume = "66",
number = "5-6",
pages = "303-318",
doi = "10.1016/S0960-0760(98)00057-0"
}

Počuča, N., Ruždijić, S., Demonacos, C., Kanazir, D. T.,& Krstić-Demonacos, M.. (1998). Using yeast to study glucocorticoid receptor phosphorylation. in Journal of Steroid Biochemistry and Molecular Biology, 66(5-6), 303-318.
https://doi.org/10.1016/S0960-0760(98)00057-0

Počuča N, Ruždijić S, Demonacos C, Kanazir DT, Krstić-Demonacos M. Using yeast to study glucocorticoid receptor phosphorylation. in Journal of Steroid Biochemistry and Molecular Biology. 1998;66(5-6):303-318.
doi:10.1016/S0960-0760(98)00057-0 .

Počuča, Nataša, Ruždijić, Sabera, Demonacos, Constantinos, Kanazir, Dušan T., Krstić-Demonacos, Marija, "Using yeast to study glucocorticoid receptor phosphorylation" in Journal of Steroid Biochemistry and Molecular Biology, 66, no. 5-6 (1998):303-318,
https://doi.org/10.1016/S0960-0760(98)00057-0 . .