TY  - JOUR
AU  - Stojanović, Gordana
AU  - Stanković, Miroslava
AU  - Stojanovic, Igor Z.
AU  - Palic, Ivan
AU  - Milovanović, Vesna
AU  - Rancic, Sofija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5969
AB  - Three lichen secondary metabolites atranorin (1), evernic acid (2), and usnic acid (3), were evaluated for their in vitro clastogenic and antiproliferative effects on human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay at concentrations of 2 mu g/mL, 4 mu g/mL and 6 mu g/mL of final culture solution. The frequency of micronucleus (MN) was scored in binucleated cells, and cytokinesis-block proliferation index (CBPI) was calculated. Among the tested compounds, 3 exhibited the most prominent effect decreasing the frequency of MN in the range of 42.5% - 48.9%, that is about double of the positive control amifostin WR-2721 that reduces MN frequency for 22.0%. The effect of evernic acid was approximately equal to action of amifostin (23.2% -32.9%). Atranorin at concentrations of 2 mu g/mL and 4 mu g/mL decreasing the frequency of MN only for 11.1% and 1.8%, while in concentration of 6 mu g/mL increases the frequency of MN for 9.6 %. The comparable CBPI values of the investigated compounds and control suggested that they did not show a statistically significant inhibitory effect on lymphocyte cell proliferation at applied concentrations.
T2  - Natural Product Communications
T1  - Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes
VL  - 9
IS  - 4
SP  - 503
EP  - 504
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5969
ER  - 

@article{
author = "Stojanović, Gordana and Stanković, Miroslava and Stojanovic, Igor Z. and Palic, Ivan and Milovanović, Vesna and Rancic, Sofija",
year = "2014",
abstract = "Three lichen secondary metabolites atranorin (1), evernic acid (2), and usnic acid (3), were evaluated for their in vitro clastogenic and antiproliferative effects on human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay at concentrations of 2 mu g/mL, 4 mu g/mL and 6 mu g/mL of final culture solution. The frequency of micronucleus (MN) was scored in binucleated cells, and cytokinesis-block proliferation index (CBPI) was calculated. Among the tested compounds, 3 exhibited the most prominent effect decreasing the frequency of MN in the range of 42.5% - 48.9%, that is about double of the positive control amifostin WR-2721 that reduces MN frequency for 22.0%. The effect of evernic acid was approximately equal to action of amifostin (23.2% -32.9%). Atranorin at concentrations of 2 mu g/mL and 4 mu g/mL decreasing the frequency of MN only for 11.1% and 1.8%, while in concentration of 6 mu g/mL increases the frequency of MN for 9.6 %. The comparable CBPI values of the investigated compounds and control suggested that they did not show a statistically significant inhibitory effect on lymphocyte cell proliferation at applied concentrations.",
journal = "Natural Product Communications",
title = "Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes",
volume = "9",
number = "4",
pages = "503-504",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5969"
}

Stojanović, G., Stanković, M., Stojanovic, I. Z., Palic, I., Milovanović, V.,& Rancic, S.. (2014). Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes. in Natural Product Communications, 9(4), 503-504.
https://hdl.handle.net/21.15107/rcub_vinar_5969

Stojanović G, Stanković M, Stojanovic IZ, Palic I, Milovanović V, Rancic S. Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes. in Natural Product Communications. 2014;9(4):503-504.
https://hdl.handle.net/21.15107/rcub_vinar_5969 .

Stojanović, Gordana, Stanković, Miroslava, Stojanovic, Igor Z., Palic, Ivan, Milovanović, Vesna, Rancic, Sofija, "Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes" in Natural Product Communications, 9, no. 4 (2014):503-504,
https://hdl.handle.net/21.15107/rcub_vinar_5969 .

TY  - JOUR
AU  - Milovanović, Vesna
AU  - Radulovic, Niko
AU  - Todorović, Zoran
AU  - Stanković, Miroslava
AU  - Stojanović, Gordana
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3279
AB  - The hydro-alcoholic extracts of five Equisetum species, E. arvense L., E. sylvaticum L., E. fluviatile L., E. palustre L. and E. telmateia Ehrh., growing-wild in Serbia were evaluated for their genotoxicity, antimicrobial activity, antioxidant capacity and the results related to the total phenol content and HPLC flavonoid profiles. The total phenol content was 92-349 mu mol expressed as equivalents of chlorogenic acid per g of dried plant material. Main identified compounds were kaempferol-, quercetin- glycosides and caffeic acid derivatives. E. telmateia extract showed the greatest antioxidant capacity. Almost all tested microorganisms demonstrated some degree of sensitivity to the examined extracts. All tested extracts at 62.5 mu g/ml showed higher incidence of micronucleus formation than in the control sample. The obtained data allowed mutual comparison of examined species and their assessment as possible sources of antioxidants, antimicrobials and/or genotoxic substances.
T2  - Plant Foods for Human Nutrition
T1  - Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species
VL  - 62
IS  - 3
SP  - 113
EP  - 119
DO  - 10.1007/s11130-007-0050-z
ER  - 

@article{
author = "Milovanović, Vesna and Radulovic, Niko and Todorović, Zoran and Stanković, Miroslava and Stojanović, Gordana",
year = "2007",
abstract = "The hydro-alcoholic extracts of five Equisetum species, E. arvense L., E. sylvaticum L., E. fluviatile L., E. palustre L. and E. telmateia Ehrh., growing-wild in Serbia were evaluated for their genotoxicity, antimicrobial activity, antioxidant capacity and the results related to the total phenol content and HPLC flavonoid profiles. The total phenol content was 92-349 mu mol expressed as equivalents of chlorogenic acid per g of dried plant material. Main identified compounds were kaempferol-, quercetin- glycosides and caffeic acid derivatives. E. telmateia extract showed the greatest antioxidant capacity. Almost all tested microorganisms demonstrated some degree of sensitivity to the examined extracts. All tested extracts at 62.5 mu g/ml showed higher incidence of micronucleus formation than in the control sample. The obtained data allowed mutual comparison of examined species and their assessment as possible sources of antioxidants, antimicrobials and/or genotoxic substances.",
journal = "Plant Foods for Human Nutrition",
title = "Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species",
volume = "62",
number = "3",
pages = "113-119",
doi = "10.1007/s11130-007-0050-z"
}

Milovanović, V., Radulovic, N., Todorović, Z., Stanković, M.,& Stojanović, G.. (2007). Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species. in Plant Foods for Human Nutrition, 62(3), 113-119.
https://doi.org/10.1007/s11130-007-0050-z

Milovanović V, Radulovic N, Todorović Z, Stanković M, Stojanović G. Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species. in Plant Foods for Human Nutrition. 2007;62(3):113-119.
doi:10.1007/s11130-007-0050-z .

Milovanović, Vesna, Radulovic, Niko, Todorović, Zoran, Stanković, Miroslava, Stojanović, Gordana, "Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species" in Plant Foods for Human Nutrition, 62, no. 3 (2007):113-119,
https://doi.org/10.1007/s11130-007-0050-z . .