TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Milutinovic, Danijela Vojnovic
AU  - Macut, Djuro
AU  - Stojiljković, Mojca D.
AU  - Nikolić, Marina
AU  - Bozic-Antic, Ivana
AU  - Ćulafić, Tijana
AU  - Bjekic-Macut, Jelica
AU  - Matić, Gordana
AU  - Korićanac, Goran
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/710
AB  - Polycystic ovary syndrome (PCOS) is associated with an altered plasma lipid profile and increased risk for cardiovascular diseases. We hypothesized that molecular mechanisms underlying cardiac pathology in PCOS involve changes in expression and subcellular localization of several key proteins involved in cardiac lipid transport and metabolism, such as fatty acid transporter CD36, lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 (PGC1), and carnitine palmitoyltransferase 1 (CPT1). We used the animal model of PCOS obtained by treating female rats with dihydrotestosterone (DHT). Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative enzymes were assessed by Western blot in different cardiac cell compartments. Cardiac triglycerides (TG) and lipid peroxidation were also measured. The content of CD36 was decreased in both the cardiac plasma membranes and intracellular pool. On the other hand, total content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast to decreased microsomal lipin 1 content. An increase in nuclear content of lipin 1 was observed together with elevation of nuclear PPAR alpha and PGC1, and an increase in CPT1 expression. However, lipid peroxidation was reduced in the heart, without alterations in antioxidative enzymes expression and cardiac TG content. The results indicate that treatment of female rats with DHT is accompanied by a decrease of fatty acid uptake and a reduction of lipid peroxidation in the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1 expression suggests that cardiac fatty acid metabolism is shifted toward mitochondrial beta oxidation.
T2  - Endocrine
T1  - Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome
VL  - 50
IS  - 1
SP  - 193
EP  - 201
DO  - 10.1007/s12020-015-0558-1
ER  - 

@article{
author = "Tepavčević, Snežana and Milutinovic, Danijela Vojnovic and Macut, Djuro and Stojiljković, Mojca D. and Nikolić, Marina and Bozic-Antic, Ivana and Ćulafić, Tijana and Bjekic-Macut, Jelica and Matić, Gordana and Korićanac, Goran",
year = "2015",
abstract = "Polycystic ovary syndrome (PCOS) is associated with an altered plasma lipid profile and increased risk for cardiovascular diseases. We hypothesized that molecular mechanisms underlying cardiac pathology in PCOS involve changes in expression and subcellular localization of several key proteins involved in cardiac lipid transport and metabolism, such as fatty acid transporter CD36, lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 (PGC1), and carnitine palmitoyltransferase 1 (CPT1). We used the animal model of PCOS obtained by treating female rats with dihydrotestosterone (DHT). Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative enzymes were assessed by Western blot in different cardiac cell compartments. Cardiac triglycerides (TG) and lipid peroxidation were also measured. The content of CD36 was decreased in both the cardiac plasma membranes and intracellular pool. On the other hand, total content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast to decreased microsomal lipin 1 content. An increase in nuclear content of lipin 1 was observed together with elevation of nuclear PPAR alpha and PGC1, and an increase in CPT1 expression. However, lipid peroxidation was reduced in the heart, without alterations in antioxidative enzymes expression and cardiac TG content. The results indicate that treatment of female rats with DHT is accompanied by a decrease of fatty acid uptake and a reduction of lipid peroxidation in the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1 expression suggests that cardiac fatty acid metabolism is shifted toward mitochondrial beta oxidation.",
journal = "Endocrine",
title = "Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome",
volume = "50",
number = "1",
pages = "193-201",
doi = "10.1007/s12020-015-0558-1"
}

Tepavčević, S., Milutinovic, D. V., Macut, D., Stojiljković, M. D., Nikolić, M., Bozic-Antic, I., Ćulafić, T., Bjekic-Macut, J., Matić, G.,& Korićanac, G.. (2015). Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome. in Endocrine, 50(1), 193-201.
https://doi.org/10.1007/s12020-015-0558-1

Tepavčević S, Milutinovic DV, Macut D, Stojiljković MD, Nikolić M, Bozic-Antic I, Ćulafić T, Bjekic-Macut J, Matić G, Korićanac G. Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome. in Endocrine. 2015;50(1):193-201.
doi:10.1007/s12020-015-0558-1 .

Tepavčević, Snežana, Milutinovic, Danijela Vojnovic, Macut, Djuro, Stojiljković, Mojca D., Nikolić, Marina, Bozic-Antic, Ivana, Ćulafić, Tijana, Bjekic-Macut, Jelica, Matić, Gordana, Korićanac, Goran, "Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome" in Endocrine, 50, no. 1 (2015):193-201,
https://doi.org/10.1007/s12020-015-0558-1 . .

TY  - JOUR
AU  - Matić, Gordana
AU  - Milutinovic, Danijela Vojnovic
AU  - Nestorov, Jelena
AU  - Elaković, Ivana
AU  - Jovanovic, Sanja Manitasevic
AU  - Elzaedi, Younis Mouftah
AU  - Perisic, Tatjana
AU  - Dunderski, Jadranka
AU  - Damjanović, Svetozar S.
AU  - Knežević, Goran
AU  - Spiric, Zeljko
AU  - Vermetten, Eric
AU  - Savić, Danka A.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5909
AB  - Alterations in the number and functional status of mineralocorticoid (MR) and glucocorticoid receptors (GR) may contribute to vulnerability to posttraumatic stress disorder (PTSD). Corticosteroid receptors are chaperoned by heat shock proteins Hsp90 and Hsp70. We examined relations between corticosteroid receptor and heat shock protein expression levels, and related them with war trauma exposure, PTSD and resilience to PTSD. Relative levels of MR. Hsp90 and Hsp70 were determined by immunoblotting in lymphocytes from war trauma-exposed men with current PTSD (current PTSD group, n=113), with lifetime PTSD (life-time PTSD group, n=61) and without PTSD (trauma control group, n=88), and from non-traumatized healthy controls (healthy control group, n=85). Between-group differences in MR, Hsp90 and Hsp70 levels and in MR/GR ratio were not observed. The level of MR was correlated with both Hsp90 and Hsp70 levels in trauma control and healthy control groups. On the other hand, GR level was correlated only with Hsp90 level, and this correlation was evident in current PTSD and trauma control groups. In conclusion, PTSD and exposure to trauma are not related to changes in lymphocyte MR, Hsp90 or Hsp70 levels, but may be associated with disturbances in corticosteroid receptors interaction with heat shock proteins. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
T2  - Psychiatry Research
T1  - Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD
VL  - 215
IS  - 2
SP  - 379
EP  - 385
DO  - 10.1016/j.psychres.2013.11.022
ER  - 

@article{
author = "Matić, Gordana and Milutinovic, Danijela Vojnovic and Nestorov, Jelena and Elaković, Ivana and Jovanovic, Sanja Manitasevic and Elzaedi, Younis Mouftah and Perisic, Tatjana and Dunderski, Jadranka and Damjanović, Svetozar S. and Knežević, Goran and Spiric, Zeljko and Vermetten, Eric and Savić, Danka A.",
year = "2014",
abstract = "Alterations in the number and functional status of mineralocorticoid (MR) and glucocorticoid receptors (GR) may contribute to vulnerability to posttraumatic stress disorder (PTSD). Corticosteroid receptors are chaperoned by heat shock proteins Hsp90 and Hsp70. We examined relations between corticosteroid receptor and heat shock protein expression levels, and related them with war trauma exposure, PTSD and resilience to PTSD. Relative levels of MR. Hsp90 and Hsp70 were determined by immunoblotting in lymphocytes from war trauma-exposed men with current PTSD (current PTSD group, n=113), with lifetime PTSD (life-time PTSD group, n=61) and without PTSD (trauma control group, n=88), and from non-traumatized healthy controls (healthy control group, n=85). Between-group differences in MR, Hsp90 and Hsp70 levels and in MR/GR ratio were not observed. The level of MR was correlated with both Hsp90 and Hsp70 levels in trauma control and healthy control groups. On the other hand, GR level was correlated only with Hsp90 level, and this correlation was evident in current PTSD and trauma control groups. In conclusion, PTSD and exposure to trauma are not related to changes in lymphocyte MR, Hsp90 or Hsp70 levels, but may be associated with disturbances in corticosteroid receptors interaction with heat shock proteins. (C) 2013 Elsevier Ireland Ltd. All rights reserved.",
journal = "Psychiatry Research",
title = "Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD",
volume = "215",
number = "2",
pages = "379-385",
doi = "10.1016/j.psychres.2013.11.022"
}

Matić, G., Milutinovic, D. V., Nestorov, J., Elaković, I., Jovanovic, S. M., Elzaedi, Y. M., Perisic, T., Dunderski, J., Damjanović, S. S., Knežević, G., Spiric, Z., Vermetten, E.,& Savić, D. A.. (2014). Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD. in Psychiatry Research, 215(2), 379-385.
https://doi.org/10.1016/j.psychres.2013.11.022

Matić G, Milutinovic DV, Nestorov J, Elaković I, Jovanovic SM, Elzaedi YM, Perisic T, Dunderski J, Damjanović SS, Knežević G, Spiric Z, Vermetten E, Savić DA. Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD. in Psychiatry Research. 2014;215(2):379-385.
doi:10.1016/j.psychres.2013.11.022 .

Matić, Gordana, Milutinovic, Danijela Vojnovic, Nestorov, Jelena, Elaković, Ivana, Jovanovic, Sanja Manitasevic, Elzaedi, Younis Mouftah, Perisic, Tatjana, Dunderski, Jadranka, Damjanović, Svetozar S., Knežević, Goran, Spiric, Zeljko, Vermetten, Eric, Savić, Danka A., "Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD" in Psychiatry Research, 215, no. 2 (2014):379-385,
https://doi.org/10.1016/j.psychres.2013.11.022 . .

TY  - JOUR
AU  - Matić, Gordana
AU  - Milutinovic, Danijela Vojnovic
AU  - Nestorov, Jelena
AU  - Elaković, Ivana
AU  - Jovanovic, Sanja Manitasevic
AU  - Perisic, Tatjana
AU  - Dunderski, Jadranka
AU  - Damjanović, Svetozar S.
AU  - Knežević, Goran
AU  - Spiric, Zeljko
AU  - Vermetten, Eric
AU  - Savić, Danka A.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5429
AB  - Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Progress in Neuro-psychopharmacology and Biological Psychiatry
T1  - Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD
VL  - 43
SP  - 238
EP  - 245
DO  - 10.1016/j.pnpbp.2013.01.005
ER  - 

@article{
author = "Matić, Gordana and Milutinovic, Danijela Vojnovic and Nestorov, Jelena and Elaković, Ivana and Jovanovic, Sanja Manitasevic and Perisic, Tatjana and Dunderski, Jadranka and Damjanović, Svetozar S. and Knežević, Goran and Spiric, Zeljko and Vermetten, Eric and Savić, Danka A.",
year = "2013",
abstract = "Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Progress in Neuro-psychopharmacology and Biological Psychiatry",
title = "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD",
volume = "43",
pages = "238-245",
doi = "10.1016/j.pnpbp.2013.01.005"
}

Matić, G., Milutinovic, D. V., Nestorov, J., Elaković, I., Jovanovic, S. M., Perisic, T., Dunderski, J., Damjanović, S. S., Knežević, G., Spiric, Z., Vermetten, E.,& Savić, D. A.. (2013). Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-psychopharmacology and Biological Psychiatry, 43, 238-245.
https://doi.org/10.1016/j.pnpbp.2013.01.005

Matić G, Milutinovic DV, Nestorov J, Elaković I, Jovanovic SM, Perisic T, Dunderski J, Damjanović SS, Knežević G, Spiric Z, Vermetten E, Savić DA. Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-psychopharmacology and Biological Psychiatry. 2013;43:238-245.
doi:10.1016/j.pnpbp.2013.01.005 .

Matić, Gordana, Milutinovic, Danijela Vojnovic, Nestorov, Jelena, Elaković, Ivana, Jovanovic, Sanja Manitasevic, Perisic, Tatjana, Dunderski, Jadranka, Damjanović, Svetozar S., Knežević, Goran, Spiric, Zeljko, Vermetten, Eric, Savić, Danka A., "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD" in Progress in Neuro-psychopharmacology and Biological Psychiatry, 43 (2013):238-245,
https://doi.org/10.1016/j.pnpbp.2013.01.005 . .