TY  - JOUR
AU  - Laban, Bojana B.
AU  - Lazarević-Pašti, Tamara
AU  - Veljović, Đorđe
AU  - Marković, Mirjana
AU  - Klekotka, Urszula
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10740
AB  - The silver and gold L-methionine capped nanoparticles (Ag and Au @LM NPs) were analyzed as prospective acetylcholinesterase (AChE) inhibitors to test their potential in the treatment of cognitive impairment in depression and Alzheimer's disease. The stability of NPs, and their ability to inhibit AChE were studied by UV-Vis and FTIR spectrophotometry. At the same time, TEM and SEM measurements, DLS, and zeta potential measurements were employed in the structural characterization of NPs. Nearly spherical, negatively charged Ag and Au @LM NPs, with 17 nm and 31 nm in diameter, respectively, showed moderate inhibitory potential toward AChE in the given frame of investigated concentrations. For both NPs IC50 is not reached. Furthermore, the adsorption of enzyme molecules on the surface of Ag and Au @LM NPs was demonstrated. Hence, our assumption is that inhibition of AChE is caused by blockage of the enzyme‘s active site due to the steric hindrance of NPs.
T2  - European Journal of Inorganic Chemistry
T1  - Methionine Capped Nanoparticles as Acetylcholinesterase Inhibitors
SP  - e202200754
DO  - 10.1002/ejic.202200754
ER  - 

@article{
author = "Laban, Bojana B. and Lazarević-Pašti, Tamara and Veljović, Đorđe and Marković, Mirjana and Klekotka, Urszula",
year = "2023",
abstract = "The silver and gold L-methionine capped nanoparticles (Ag and Au @LM NPs) were analyzed as prospective acetylcholinesterase (AChE) inhibitors to test their potential in the treatment of cognitive impairment in depression and Alzheimer's disease. The stability of NPs, and their ability to inhibit AChE were studied by UV-Vis and FTIR spectrophotometry. At the same time, TEM and SEM measurements, DLS, and zeta potential measurements were employed in the structural characterization of NPs. Nearly spherical, negatively charged Ag and Au @LM NPs, with 17 nm and 31 nm in diameter, respectively, showed moderate inhibitory potential toward AChE in the given frame of investigated concentrations. For both NPs IC50 is not reached. Furthermore, the adsorption of enzyme molecules on the surface of Ag and Au @LM NPs was demonstrated. Hence, our assumption is that inhibition of AChE is caused by blockage of the enzyme‘s active site due to the steric hindrance of NPs.",
journal = "European Journal of Inorganic Chemistry",
title = "Methionine Capped Nanoparticles as Acetylcholinesterase Inhibitors",
pages = "e202200754",
doi = "10.1002/ejic.202200754"
}

Laban, B. B., Lazarević-Pašti, T., Veljović, Đ., Marković, M.,& Klekotka, U.. (2023). Methionine Capped Nanoparticles as Acetylcholinesterase Inhibitors. in European Journal of Inorganic Chemistry, e202200754.
https://doi.org/10.1002/ejic.202200754

Laban BB, Lazarević-Pašti T, Veljović Đ, Marković M, Klekotka U. Methionine Capped Nanoparticles as Acetylcholinesterase Inhibitors. in European Journal of Inorganic Chemistry. 2023;:e202200754.
doi:10.1002/ejic.202200754 .

Laban, Bojana B., Lazarević-Pašti, Tamara, Veljović, Đorđe, Marković, Mirjana, Klekotka, Urszula, "Methionine Capped Nanoparticles as Acetylcholinesterase Inhibitors" in European Journal of Inorganic Chemistry (2023):e202200754,
https://doi.org/10.1002/ejic.202200754 . .

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Jovanović, Dunja
AU  - Arsenijević, Nevena
AU  - Laban, Bojana
AU  - Lazarević-Pašti, Tamara
AU  - Klekotka, Urszula
AU  - Bondžić, Bojan P.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10404
AB  - The study of the interactions between nanoparticles (NPs) and proteins has had a pivotal role in facilitating the understanding of biological effects and safe application of NPs after exposure to the physiological environment. Herein, for the first time, the interaction between L-methionine capped silver nanoparticles (AgMet), and bovine serum albumin (BSA) is investigated in order to predict the fate of AgMet after its contact with the most abundant blood transport protein. The detailed insights into the mechanism of interaction were achieved using different physicochemical techniques. The UV/Vis, TEM, and DLS were used for the characterization of the newly formed “entity”, while the kinetic and thermodynamic parameters were utilized to describe the adsorption process. Additionally, the fluorescence quenching and synchronous fluorescence studies enabled the prediction of the binding affinity and gave us insight into the influence of the adsorption on the conformation state of the BSA. According to the best of our knowledge, for the first time, we show that BSA can be used as an external stabilizer agent which is able to induce the peptization of previously agglomerated AgMet. We believe that the obtained results could contribute to further improvement of AgNPs’ performances as well as to the understanding of their in vivo behavior, which could contribute to their potential use in preclinical research studies.
T2  - International Journal of Molecular Sciences
T1  - “Soft Protein Corona” as the Stabilizer of the Methionine-Coated Silver Nanoparticles in the Physiological Environment: Insights into the Mechanism of the Interaction
VL  - 23
IS  - 16
SP  - 8985
DO  - 10.3390/ijms23168985
ER  - 

@article{
author = "Bondžić, Aleksandra M. and Jovanović, Dunja and Arsenijević, Nevena and Laban, Bojana and Lazarević-Pašti, Tamara and Klekotka, Urszula and Bondžić, Bojan P.",
year = "2022",
abstract = "The study of the interactions between nanoparticles (NPs) and proteins has had a pivotal role in facilitating the understanding of biological effects and safe application of NPs after exposure to the physiological environment. Herein, for the first time, the interaction between L-methionine capped silver nanoparticles (AgMet), and bovine serum albumin (BSA) is investigated in order to predict the fate of AgMet after its contact with the most abundant blood transport protein. The detailed insights into the mechanism of interaction were achieved using different physicochemical techniques. The UV/Vis, TEM, and DLS were used for the characterization of the newly formed “entity”, while the kinetic and thermodynamic parameters were utilized to describe the adsorption process. Additionally, the fluorescence quenching and synchronous fluorescence studies enabled the prediction of the binding affinity and gave us insight into the influence of the adsorption on the conformation state of the BSA. According to the best of our knowledge, for the first time, we show that BSA can be used as an external stabilizer agent which is able to induce the peptization of previously agglomerated AgMet. We believe that the obtained results could contribute to further improvement of AgNPs’ performances as well as to the understanding of their in vivo behavior, which could contribute to their potential use in preclinical research studies.",
journal = "International Journal of Molecular Sciences",
title = "“Soft Protein Corona” as the Stabilizer of the Methionine-Coated Silver Nanoparticles in the Physiological Environment: Insights into the Mechanism of the Interaction",
volume = "23",
number = "16",
pages = "8985",
doi = "10.3390/ijms23168985"
}

Bondžić, A. M., Jovanović, D., Arsenijević, N., Laban, B., Lazarević-Pašti, T., Klekotka, U.,& Bondžić, B. P.. (2022). “Soft Protein Corona” as the Stabilizer of the Methionine-Coated Silver Nanoparticles in the Physiological Environment: Insights into the Mechanism of the Interaction. in International Journal of Molecular Sciences, 23(16), 8985.
https://doi.org/10.3390/ijms23168985

Bondžić AM, Jovanović D, Arsenijević N, Laban B, Lazarević-Pašti T, Klekotka U, Bondžić BP. “Soft Protein Corona” as the Stabilizer of the Methionine-Coated Silver Nanoparticles in the Physiological Environment: Insights into the Mechanism of the Interaction. in International Journal of Molecular Sciences. 2022;23(16):8985.
doi:10.3390/ijms23168985 .

Bondžić, Aleksandra M., Jovanović, Dunja, Arsenijević, Nevena, Laban, Bojana, Lazarević-Pašti, Tamara, Klekotka, Urszula, Bondžić, Bojan P., "“Soft Protein Corona” as the Stabilizer of the Methionine-Coated Silver Nanoparticles in the Physiological Environment: Insights into the Mechanism of the Interaction" in International Journal of Molecular Sciences, 23, no. 16 (2022):8985,
https://doi.org/10.3390/ijms23168985 . .

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Vujačić Nikezić, Ana V.
AU  - Klekotka,  Urszula
AU  - Marković, Mirjana
AU  - Vodnik, Vesna
AU  - Kalska, B.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8812
AB  - Abstract: This paper presents the study of the interaction between gold nanoparticles (AuNPs) and antitumor gold complexes, [Au(OH)2(bipy)][PF6], [Au(CH3COO)2(pydmb-H)], and [Au(bipydmb-H)(OH)][PF6], in order to estimate the possibility for metal complex tracking in cells using nanospectroscopy approach. Decrease of intensity of the surface plasmon absorption band at 524 nm and the appearance of a new broad band at ∼640 nm, followed by their red shift were observed in the presence of the complexes. TEM images showed that the average size and shape of AuNPs did not change after the addition of gold complexes. DLS and zeta potential measurements pointed out to the metal complex adsorption on the surface of AuNPs followed by flocculation process. In addition, kinetic studies indicated strong bond formation between these complexes and AuNPs. Accordingly, AuNPs/complex conjugates have the potential to be applied for the tracking of these metal complexes in the cells using nanospectroscopy approach. © 2019, Pleiades Publishing, Ltd.
T2  - Russian Journal of Physical Chemistry A
T1  - Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles
VL  - 93
IS  - 13
SP  - 2765
EP  - 2770
DO  - 10.1134/S0036024419130065
ER  - 

@article{
author = "Bondžić, Aleksandra M. and Vujačić Nikezić, Ana V. and Klekotka,  Urszula and Marković, Mirjana and Vodnik, Vesna and Kalska, B. and Vasić, Vesna M.",
year = "2019",
abstract = "Abstract: This paper presents the study of the interaction between gold nanoparticles (AuNPs) and antitumor gold complexes, [Au(OH)2(bipy)][PF6], [Au(CH3COO)2(pydmb-H)], and [Au(bipydmb-H)(OH)][PF6], in order to estimate the possibility for metal complex tracking in cells using nanospectroscopy approach. Decrease of intensity of the surface plasmon absorption band at 524 nm and the appearance of a new broad band at ∼640 nm, followed by their red shift were observed in the presence of the complexes. TEM images showed that the average size and shape of AuNPs did not change after the addition of gold complexes. DLS and zeta potential measurements pointed out to the metal complex adsorption on the surface of AuNPs followed by flocculation process. In addition, kinetic studies indicated strong bond formation between these complexes and AuNPs. Accordingly, AuNPs/complex conjugates have the potential to be applied for the tracking of these metal complexes in the cells using nanospectroscopy approach. © 2019, Pleiades Publishing, Ltd.",
journal = "Russian Journal of Physical Chemistry A",
title = "Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles",
volume = "93",
number = "13",
pages = "2765-2770",
doi = "10.1134/S0036024419130065"
}

Bondžić, A. M., Vujačić Nikezić, A. V., Klekotka, U., Marković, M., Vodnik, V., Kalska, B.,& Vasić, V. M.. (2019). Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles. in Russian Journal of Physical Chemistry A, 93(13), 2765-2770.
https://doi.org/10.1134/S0036024419130065

Bondžić AM, Vujačić Nikezić AV, Klekotka U, Marković M, Vodnik V, Kalska B, Vasić VM. Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles. in Russian Journal of Physical Chemistry A. 2019;93(13):2765-2770.
doi:10.1134/S0036024419130065 .

Bondžić, Aleksandra M., Vujačić Nikezić, Ana V., Klekotka,  Urszula, Marković, Mirjana, Vodnik, Vesna, Kalska, B., Vasić, Vesna M., "Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles" in Russian Journal of Physical Chemistry A, 93, no. 13 (2019):2765-2770,
https://doi.org/10.1134/S0036024419130065 . .