TY  - JOUR
AU  - Stojanović, Marko
AU  - Čolović, Mirjana B.
AU  - Lalatović, Jovana
AU  - Milosavljević, Aleksandra
AU  - Savić, Nada D.
AU  - Declerck, Kilian
AU  - Radosavljević, Branimir
AU  - Ćetković, Mila
AU  - Kravić-Stevović, Tamara
AU  - Parac-Vogt, Tatjana N.
AU  - Krstić, Danijela
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12867
AB  - Polyoxotungstate nanoclusters have recently emerged as promising contrast agents for computed tomography (CT). In order to evaluate their clinical potential, in this study, we evaluated the in vitro CT imaging properties, potential toxic effects in vivo, and tissue distribution of monolacunary Wells–Dawson polyoxometalate, α2-K10P2W17O61.20H2O (mono-WD POM). Mono-WD POM showed superior X-ray attenuation compared to other tungsten-containing nanoclusters (its parent WD-POM and Keggin POM) and the standard iodine-based contrast agent (iohexol). The calculated X-ray attenuation linear slope for mono-WD POM was significantly higher compared to parent WD-POM, Keggin POM, and iohexol (5.97 ± 0.14 vs. 4.84 ± 0.05, 4.55 ± 0.16, and 4.30 ± 0.09, respectively). Acute oral (maximum-administered dose (MAD) = 960 mg/kg) and intravenous administration (1/10, 1/5, and 1/3 MAD) of mono-WD POM did not induce unexpected changes in rats’ general habits or mortality. Results of blood gas analysis, CO-oximetry status, and the levels of electrolytes, glucose, lactate, creatinine, and BUN demonstrated a dose-dependent tendency 14 days after intravenous administration of mono-WD POM. The most significant differences compared to the control were observed for 1/3 MAD, being approximately seventy times higher than the typically used dose (0.015 mmol W/kg) of tungsten-based contrast agents. The highest tungsten deposition was found in the kidney (1/3 MAD—0.67 ± 0.12; 1/5 MAD—0.59 ± 0.07; 1/10 MAD—0.54 ± 0.05), which corresponded to detected morphological irregularities, electrolyte imbalance, and increased BUN levels.
T2  - International Journal of Molecular Sciences
T1  - Monolacunary Wells-Dawson Polyoxometalate as a Novel Contrast Agent for Computed Tomography: A Comprehensive Study on In Vivo Toxicity and Biodistribution
VL  - 25
IS  - 5
SP  - 2569
DO  - 10.3390/ijms25052569
ER  - 

@article{
author = "Stojanović, Marko and Čolović, Mirjana B. and Lalatović, Jovana and Milosavljević, Aleksandra and Savić, Nada D. and Declerck, Kilian and Radosavljević, Branimir and Ćetković, Mila and Kravić-Stevović, Tamara and Parac-Vogt, Tatjana N. and Krstić, Danijela",
year = "2024",
abstract = "Polyoxotungstate nanoclusters have recently emerged as promising contrast agents for computed tomography (CT). In order to evaluate their clinical potential, in this study, we evaluated the in vitro CT imaging properties, potential toxic effects in vivo, and tissue distribution of monolacunary Wells–Dawson polyoxometalate, α2-K10P2W17O61.20H2O (mono-WD POM). Mono-WD POM showed superior X-ray attenuation compared to other tungsten-containing nanoclusters (its parent WD-POM and Keggin POM) and the standard iodine-based contrast agent (iohexol). The calculated X-ray attenuation linear slope for mono-WD POM was significantly higher compared to parent WD-POM, Keggin POM, and iohexol (5.97 ± 0.14 vs. 4.84 ± 0.05, 4.55 ± 0.16, and 4.30 ± 0.09, respectively). Acute oral (maximum-administered dose (MAD) = 960 mg/kg) and intravenous administration (1/10, 1/5, and 1/3 MAD) of mono-WD POM did not induce unexpected changes in rats’ general habits or mortality. Results of blood gas analysis, CO-oximetry status, and the levels of electrolytes, glucose, lactate, creatinine, and BUN demonstrated a dose-dependent tendency 14 days after intravenous administration of mono-WD POM. The most significant differences compared to the control were observed for 1/3 MAD, being approximately seventy times higher than the typically used dose (0.015 mmol W/kg) of tungsten-based contrast agents. The highest tungsten deposition was found in the kidney (1/3 MAD—0.67 ± 0.12; 1/5 MAD—0.59 ± 0.07; 1/10 MAD—0.54 ± 0.05), which corresponded to detected morphological irregularities, electrolyte imbalance, and increased BUN levels.",
journal = "International Journal of Molecular Sciences",
title = "Monolacunary Wells-Dawson Polyoxometalate as a Novel Contrast Agent for Computed Tomography: A Comprehensive Study on In Vivo Toxicity and Biodistribution",
volume = "25",
number = "5",
pages = "2569",
doi = "10.3390/ijms25052569"
}

Stojanović, M., Čolović, M. B., Lalatović, J., Milosavljević, A., Savić, N. D., Declerck, K., Radosavljević, B., Ćetković, M., Kravić-Stevović, T., Parac-Vogt, T. N.,& Krstić, D.. (2024). Monolacunary Wells-Dawson Polyoxometalate as a Novel Contrast Agent for Computed Tomography: A Comprehensive Study on In Vivo Toxicity and Biodistribution. in International Journal of Molecular Sciences, 25(5), 2569.
https://doi.org/10.3390/ijms25052569

Stojanović M, Čolović MB, Lalatović J, Milosavljević A, Savić ND, Declerck K, Radosavljević B, Ćetković M, Kravić-Stevović T, Parac-Vogt TN, Krstić D. Monolacunary Wells-Dawson Polyoxometalate as a Novel Contrast Agent for Computed Tomography: A Comprehensive Study on In Vivo Toxicity and Biodistribution. in International Journal of Molecular Sciences. 2024;25(5):2569.
doi:10.3390/ijms25052569 .

Stojanović, Marko, Čolović, Mirjana B., Lalatović, Jovana, Milosavljević, Aleksandra, Savić, Nada D., Declerck, Kilian, Radosavljević, Branimir, Ćetković, Mila, Kravić-Stevović, Tamara, Parac-Vogt, Tatjana N., Krstić, Danijela, "Monolacunary Wells-Dawson Polyoxometalate as a Novel Contrast Agent for Computed Tomography: A Comprehensive Study on In Vivo Toxicity and Biodistribution" in International Journal of Molecular Sciences, 25, no. 5 (2024):2569,
https://doi.org/10.3390/ijms25052569 . .

TY  - CONF
AU  - Dinčić, Marko
AU  - Todorović, Jasna
AU  - Čolović, Mirjana
AU  - Ćetković Milisavljević, Mila
AU  - Kravić Stevović, Tamara
AU  - Milosavljević, Aleksandra
AU  - Milinković, Neda
AU  - Kortz, Urlich
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12942
AB  - Zahvaljujući brojnim istraživanjima koja su ispitivala biološka svojstva strukturno različitih polioksometalata, došlo se do zapažanja da ova kompleksna neorganska jedinjenja, pored antimikrobnog i antitumorskog delovanja, mogu biti delotvorna u snižavanju hiperglikemije kod pacova sa eksperimentalno izazvanim dijabetesom. Stoga, cilj ove studije je bio da se ispitaju antidijabetički potencijal i mogući toksični efekti dva polioksovolframata:(NH4 )14[NaP5 W30O110]·31H2 O, {NaP5 W30} i K14[AgP5 W30O110]·22H2 O·6KCl, {AgP5 W30}. U cilju realizacije postavljenog cilja, korišćena su tri eksperimentalna modela: (1) antihiperglikemijska screening studija u kojoj je ispitivan uticaj jednokratne intraperitonealne primene {NaP5 W30} i {AgP5 W30} (5, 10 i 20 mg/kg) na snižavanje hiperglikemije kod dijabetičkih pacova, (2) akutna peroralna toksikološka studija koja je istraživala hepato- i nefrotoksične efekte odabranih heteropolivolframata kod zdravih pacova i (3) studija posvećena rasvetljavanju mogućih mehanizama antidijabetičkog delovanja heteropolivolframata. Rezultati screening studije su pokazali da su oba ispitivana heteropolivolframata efikasna u snižavanju hiperglikemije, s tim što se {NaP5 W30}, u odnosu na {AgP5 W30}, pokazao kao moćniji antihiperglikemijski agens. Rezultati biohemijskih parametara funkcije i patohistološka analiza jetre i bubrege korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije pokazuju da dvonedeljna primena {NaP5 W30} i {AgP5 W30} (20 mg/kg) izaziva blagi do umereni stepen hepato- i nefrotoksičnosti kod zdravih životinja. U poslednjem eksperimentalnom protokolu, pokazano je da tronedeljna peroralna primena {NaP5 W30} (20 mg/kg) povećava koncentraciju insulina u serumu dijabetičkih pacova, što može biti jedan od mehanizama njegovog antidijabetičkog delovanja. Takođe, pokazano je da {NaP5 W30} ispoljava hepato-, nefro-, kardio- i neuroprotektivno dejstvo kod dijabetičkih pacova, što je procenjeno na osnovu analize: (1) relativne mase organa, (2) biohemijskih parametara funkcije, (3) parametara oksidativnog stresa u homogenatu tkiva, (4) aktivnosti acetilholinesteraze, Na+ /K+-ATPaze i ecto-ATPaza u sinaptozomima i (5) patohistoloških promena u tkivima korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije. Stoga, {NaP5 W30} i {AgP5 W30} mogu se smatrati mogućim neinsulinskim lekovima-kandidatima u terapiji dijabetesa tipa 2, koji bi se podvrgli daljim pretkliničkim istraživanjima.
C3  - Medical Research : Medicinska istraživanja
T1  - Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera
VL  - 56
IS  - 4
SP  - 132
EP  - 133
DO  - 10.5937/medi56-47579
ER  - 

@conference{
author = "Dinčić, Marko and Todorović, Jasna and Čolović, Mirjana and Ćetković Milisavljević, Mila and Kravić Stevović, Tamara and Milosavljević, Aleksandra and Milinković, Neda and Kortz, Urlich and Krstić, Danijela",
year = "2023",
abstract = "Zahvaljujući brojnim istraživanjima koja su ispitivala biološka svojstva strukturno različitih polioksometalata, došlo se do zapažanja da ova kompleksna neorganska jedinjenja, pored antimikrobnog i antitumorskog delovanja, mogu biti delotvorna u snižavanju hiperglikemije kod pacova sa eksperimentalno izazvanim dijabetesom. Stoga, cilj ove studije je bio da se ispitaju antidijabetički potencijal i mogući toksični efekti dva polioksovolframata:(NH4 )14[NaP5 W30O110]·31H2 O, {NaP5 W30} i K14[AgP5 W30O110]·22H2 O·6KCl, {AgP5 W30}. U cilju realizacije postavljenog cilja, korišćena su tri eksperimentalna modela: (1) antihiperglikemijska screening studija u kojoj je ispitivan uticaj jednokratne intraperitonealne primene {NaP5 W30} i {AgP5 W30} (5, 10 i 20 mg/kg) na snižavanje hiperglikemije kod dijabetičkih pacova, (2) akutna peroralna toksikološka studija koja je istraživala hepato- i nefrotoksične efekte odabranih heteropolivolframata kod zdravih pacova i (3) studija posvećena rasvetljavanju mogućih mehanizama antidijabetičkog delovanja heteropolivolframata. Rezultati screening studije su pokazali da su oba ispitivana heteropolivolframata efikasna u snižavanju hiperglikemije, s tim što se {NaP5 W30}, u odnosu na {AgP5 W30}, pokazao kao moćniji antihiperglikemijski agens. Rezultati biohemijskih parametara funkcije i patohistološka analiza jetre i bubrege korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije pokazuju da dvonedeljna primena {NaP5 W30} i {AgP5 W30} (20 mg/kg) izaziva blagi do umereni stepen hepato- i nefrotoksičnosti kod zdravih životinja. U poslednjem eksperimentalnom protokolu, pokazano je da tronedeljna peroralna primena {NaP5 W30} (20 mg/kg) povećava koncentraciju insulina u serumu dijabetičkih pacova, što može biti jedan od mehanizama njegovog antidijabetičkog delovanja. Takođe, pokazano je da {NaP5 W30} ispoljava hepato-, nefro-, kardio- i neuroprotektivno dejstvo kod dijabetičkih pacova, što je procenjeno na osnovu analize: (1) relativne mase organa, (2) biohemijskih parametara funkcije, (3) parametara oksidativnog stresa u homogenatu tkiva, (4) aktivnosti acetilholinesteraze, Na+ /K+-ATPaze i ecto-ATPaza u sinaptozomima i (5) patohistoloških promena u tkivima korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije. Stoga, {NaP5 W30} i {AgP5 W30} mogu se smatrati mogućim neinsulinskim lekovima-kandidatima u terapiji dijabetesa tipa 2, koji bi se podvrgli daljim pretkliničkim istraživanjima.",
journal = "Medical Research : Medicinska istraživanja",
title = "Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera",
volume = "56",
number = "4",
pages = "132-133",
doi = "10.5937/medi56-47579"
}

Dinčić, M., Todorović, J., Čolović, M., Ćetković Milisavljević, M., Kravić Stevović, T., Milosavljević, A., Milinković, N., Kortz, U.,& Krstić, D.. (2023). Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera. in Medical Research : Medicinska istraživanja, 56(4), 132-133.
https://doi.org/10.5937/medi56-47579

Dinčić M, Todorović J, Čolović M, Ćetković Milisavljević M, Kravić Stevović T, Milosavljević A, Milinković N, Kortz U, Krstić D. Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera. in Medical Research : Medicinska istraživanja. 2023;56(4):132-133.
doi:10.5937/medi56-47579 .

Dinčić, Marko, Todorović, Jasna, Čolović, Mirjana, Ćetković Milisavljević, Mila, Kravić Stevović, Tamara, Milosavljević, Aleksandra, Milinković, Neda, Kortz, Urlich, Krstić, Danijela, "Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera" in Medical Research : Medicinska istraživanja, 56, no. 4 (2023):132-133,
https://doi.org/10.5937/medi56-47579 . .

TY  - JOUR
AU  - Stojanović, Marko
AU  - Lalatović, Jovana
AU  - Milosavljević, Aleksandra
AU  - Savić, Nada
AU  - Simms, Charlotte
AU  - Radosavljević, Branimir
AU  - Ćetković, Mila
AU  - Kravić Stevović, Tamara
AU  - Mrda, Davor
AU  - Čolović, Mirjana B.
AU  - Parac-Vogt, Tatjana N.
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11091
AB  - In this study, we demonstrate for the first time, that a discrete metal-oxo cluster α-/β-K 6 P 2 W 18 O 62 (WD-POM) exhibits superior performance as a computed tomography (CT) contrast agent, in comparison to the standard contrast agent iohexol. A toxicity evaluation of WD-POM was performed according to standard toxicological protocols using Wistar albino rats. The maximum tolerable dose (MTD) of 2000 mg/kg was initially determined after oral WD-POM application. The acute intravenous toxicity of single WD-POM doses (1/3, 1/5, and 1/10 MTD), which are at least fifty times higher than the typically used dose (0.015 mmol W kg −1 ) of tungsten-based contrast agents, was evaluated for 14 days. The results of arterial blood gas analysis, CO-oximetry status, electrolyte and lactate levels for 1/10 MTD group (80% survival rate) indicated the mixed respiratory and metabolic acidosis. The highest deposition of WD-POM (0.6 ppm tungsten) was found in the kidney, followed by liver (0.15 ppm tungsten), for which the histological analysis revealed morphological irregularities, although the renal function parameters (creatinine and BUN levels) were within the physiological range. This study is the first and important step in evaluating side effects of polyoxometalate nanoclusters, which in recent years have shown a large potential as therapeutics and contrast agents.
T2  - Scientific Reports
T1  - In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography
VL  - 13
IS  - 1
SP  - 9140
DO  - 10.1038/s41598-023-36317-8
ER  - 

@article{
author = "Stojanović, Marko and Lalatović, Jovana and Milosavljević, Aleksandra and Savić, Nada and Simms, Charlotte and Radosavljević, Branimir and Ćetković, Mila and Kravić Stevović, Tamara and Mrda, Davor and Čolović, Mirjana B. and Parac-Vogt, Tatjana N. and Krstić, Danijela",
year = "2023",
abstract = "In this study, we demonstrate for the first time, that a discrete metal-oxo cluster α-/β-K 6 P 2 W 18 O 62 (WD-POM) exhibits superior performance as a computed tomography (CT) contrast agent, in comparison to the standard contrast agent iohexol. A toxicity evaluation of WD-POM was performed according to standard toxicological protocols using Wistar albino rats. The maximum tolerable dose (MTD) of 2000 mg/kg was initially determined after oral WD-POM application. The acute intravenous toxicity of single WD-POM doses (1/3, 1/5, and 1/10 MTD), which are at least fifty times higher than the typically used dose (0.015 mmol W kg −1 ) of tungsten-based contrast agents, was evaluated for 14 days. The results of arterial blood gas analysis, CO-oximetry status, electrolyte and lactate levels for 1/10 MTD group (80% survival rate) indicated the mixed respiratory and metabolic acidosis. The highest deposition of WD-POM (0.6 ppm tungsten) was found in the kidney, followed by liver (0.15 ppm tungsten), for which the histological analysis revealed morphological irregularities, although the renal function parameters (creatinine and BUN levels) were within the physiological range. This study is the first and important step in evaluating side effects of polyoxometalate nanoclusters, which in recent years have shown a large potential as therapeutics and contrast agents.",
journal = "Scientific Reports",
title = "In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography",
volume = "13",
number = "1",
pages = "9140",
doi = "10.1038/s41598-023-36317-8"
}

Stojanović, M., Lalatović, J., Milosavljević, A., Savić, N., Simms, C., Radosavljević, B., Ćetković, M., Kravić Stevović, T., Mrda, D., Čolović, M. B., Parac-Vogt, T. N.,& Krstić, D.. (2023). In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography. in Scientific Reports, 13(1), 9140.
https://doi.org/10.1038/s41598-023-36317-8

Stojanović M, Lalatović J, Milosavljević A, Savić N, Simms C, Radosavljević B, Ćetković M, Kravić Stevović T, Mrda D, Čolović MB, Parac-Vogt TN, Krstić D. In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography. in Scientific Reports. 2023;13(1):9140.
doi:10.1038/s41598-023-36317-8 .

Stojanović, Marko, Lalatović, Jovana, Milosavljević, Aleksandra, Savić, Nada, Simms, Charlotte, Radosavljević, Branimir, Ćetković, Mila, Kravić Stevović, Tamara, Mrda, Davor, Čolović, Mirjana B., Parac-Vogt, Tatjana N., Krstić, Danijela, "In vivo toxicity evaluation of a polyoxotungstate nanocluster as a promising contrast agent for computed tomography" in Scientific Reports, 13, no. 1 (2023):9140,
https://doi.org/10.1038/s41598-023-36317-8 . .

TY  - CONF
AU  - Tasić, Jelena
AU  - Vidičević Novaković, Sašenka
AU  - Stanojević, Željka
AU  - Paunović, Verica
AU  - Petričević, Saša
AU  - Martinović, Tamara
AU  - Kravić-Stevović, Tamara
AU  - Ćirić, Darko
AU  - Marković, Zoran J.
AU  - Isaković, Aleksandra
AU  - Trajković, Vladimir
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11057
AB  - Background: Experimental autoimmune encephalomyelitis (EAE) is one of the most studied model of neuroinflammation, used to test immunomodulatory and antiinflammatory drugs. Graphene quantum dots (GQD) are oval graphite twodimensional sheets with a diameter <100 nm, one carbon atom thickness, with potential applications in biomedicine. Objective: To investigate the potential protective effect of GQD in EAE model. Methods: Female DA rats were immunized with spinal cord homogenate and Freund’s complete adjuvant. GQD treatment (10 mg/kg, ip) was administrated during the inductive, effector and both phases of a disease. MAP kinase (MAPK) and Akt activity in popliteal lymph nodes (PLN) and CNS was determined by western blot. Quantitative PCR and flow cytometry were used to examine the expression of proinflammatory cytokines and specific transcription factors while infiltration of GQD in cells/tissues was detected by transmission electron microscopy. GQD antiinflamatory/direct cytoprotective effect was analyzed on oligodendrocyte and neuron cell cultures by MTT assay. Data were analized by Mann Whitney test (p<0.05 was considered as statistical significant difference). Results: GQD administration, in all phases of EAE, significantly reduced clinical score of a disease. Clinical improvement correlates with increase in activity of ERK, p38 and Akt that is followed by reduction of Th1 cell response in PLN and infiltrated spinal coard T cells. Due to its capacity to infiltrate cells and tissues, GQD exhibits direct cytoprotective effect on CNS. Additionaly, GQD reduced the expression of proinflammatory cytokines in ConA stimulated lymphocytes. Conclusion: GQD alleviate EAE, through direct cytoprotective effect on CNS and inhibition of Th1 cell response.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Graphene Quantum Dots show protective effect in animal model of neuroinflammation
SP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11057
ER  - 

@conference{
author = "Tasić, Jelena and Vidičević Novaković, Sašenka and Stanojević, Željka and Paunović, Verica and Petričević, Saša and Martinović, Tamara and Kravić-Stevović, Tamara and Ćirić, Darko and Marković, Zoran J. and Isaković, Aleksandra and Trajković, Vladimir",
year = "2023",
abstract = "Background: Experimental autoimmune encephalomyelitis (EAE) is one of the most studied model of neuroinflammation, used to test immunomodulatory and antiinflammatory drugs. Graphene quantum dots (GQD) are oval graphite twodimensional sheets with a diameter <100 nm, one carbon atom thickness, with potential applications in biomedicine. Objective: To investigate the potential protective effect of GQD in EAE model. Methods: Female DA rats were immunized with spinal cord homogenate and Freund’s complete adjuvant. GQD treatment (10 mg/kg, ip) was administrated during the inductive, effector and both phases of a disease. MAP kinase (MAPK) and Akt activity in popliteal lymph nodes (PLN) and CNS was determined by western blot. Quantitative PCR and flow cytometry were used to examine the expression of proinflammatory cytokines and specific transcription factors while infiltration of GQD in cells/tissues was detected by transmission electron microscopy. GQD antiinflamatory/direct cytoprotective effect was analyzed on oligodendrocyte and neuron cell cultures by MTT assay. Data were analized by Mann Whitney test (p<0.05 was considered as statistical significant difference). Results: GQD administration, in all phases of EAE, significantly reduced clinical score of a disease. Clinical improvement correlates with increase in activity of ERK, p38 and Akt that is followed by reduction of Th1 cell response in PLN and infiltrated spinal coard T cells. Due to its capacity to infiltrate cells and tissues, GQD exhibits direct cytoprotective effect on CNS. Additionaly, GQD reduced the expression of proinflammatory cytokines in ConA stimulated lymphocytes. Conclusion: GQD alleviate EAE, through direct cytoprotective effect on CNS and inhibition of Th1 cell response.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Graphene Quantum Dots show protective effect in animal model of neuroinflammation",
pages = "114",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11057"
}

Tasić, J., Vidičević Novaković, S., Stanojević, Ž., Paunović, V., Petričević, S., Martinović, T., Kravić-Stevović, T., Ćirić, D., Marković, Z. J., Isaković, A.,& Trajković, V.. (2023). Graphene Quantum Dots show protective effect in animal model of neuroinflammation. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 114.
https://hdl.handle.net/21.15107/rcub_vinar_11057

Tasić J, Vidičević Novaković S, Stanojević Ž, Paunović V, Petričević S, Martinović T, Kravić-Stevović T, Ćirić D, Marković ZJ, Isaković A, Trajković V. Graphene Quantum Dots show protective effect in animal model of neuroinflammation. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:114.
https://hdl.handle.net/21.15107/rcub_vinar_11057 .

Tasić, Jelena, Vidičević Novaković, Sašenka, Stanojević, Željka, Paunović, Verica, Petričević, Saša, Martinović, Tamara, Kravić-Stevović, Tamara, Ćirić, Darko, Marković, Zoran J., Isaković, Aleksandra, Trajković, Vladimir, "Graphene Quantum Dots show protective effect in animal model of neuroinflammation" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):114,
https://hdl.handle.net/21.15107/rcub_vinar_11057 .

TY  - JOUR
AU  - Dinčić, Marko
AU  - Čolović, Mirjana B.
AU  - Sarić Matutinović, Marija
AU  - Ćetković, Mila
AU  - Kravić-Stevović, Tamara K.
AU  - Mougharbel, Ali S.
AU  - Todorović, Jasna
AU  - Ignjatović, Svetlana
AU  - Radosavljević, Branimir
AU  - Milisavljević, Milan
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8479
AB  - In this study, thein vivohypoglycemic effect of a donut-shaped polyanion salt (NH4)14[Na@P5W30O110]$31H2O{NaP5W30} and its Ag-containing derivative K14[Ag@P5W30O110]$22H2O$6KCl {AgP5W30}, as wellas their hepatotoxicity and nephrotoxicity, was evaluated. In the screening hypoglycemic study,Wistaralbinorats with streptozotocin induced diabetes were treated intraperitoneally with three single doses (5,10, and 20 mg per kg per b.w.) of both investigated polyoxotungstates. The blood glucose levels,measured before and after 2, 4 and 6 h polyoxotungstate application, showed that both studiedcompounds induced the most pronounced and time dependent glucose lowering effects at the doses of20 mg kg1. Thus, daily doses of 20 mg kg1were administered toWistar albinorats orally for 14 days infurther toxicity examinations. The serum glucose concentration and biochemical parameters of kidneyand liver function, as well as a histopathological analysis of kidney and liver tissues were evaluated 14days after the polyoxotungstate administration. Both investigated compounds did not induce statisticallysignificant alterations of the serum glucose and uric acid concentrations, as well as some of the liverfunction markers (serum alanine and aspartate aminotransferases, and alkaline phosphatase activities).However, the significant decrease in serum total protein and albumin concentrations and the increase inbiochemical parameters of renal function–serum urea (up to 63.1%) and creatinine concentrations (upto 23.3%) were observed for both polyoxotungstates. In addition, the detected biochemical changeswere in accordance with kidney and liver histhopathological analysis. Accordingly, the hepatotoxic andnephrotoxic effects of these potential antidiabetic polyoxotungstates could be considered as mild.
T2  - RSC Advances
T1  - In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system
VL  - 10
IS  - 5
SP  - 2846
EP  - 2855
DO  - 10.1039/C9RA09790B
ER  - 

@article{
author = "Dinčić, Marko and Čolović, Mirjana B. and Sarić Matutinović, Marija and Ćetković, Mila and Kravić-Stevović, Tamara K. and Mougharbel, Ali S. and Todorović, Jasna and Ignjatović, Svetlana and Radosavljević, Branimir and Milisavljević, Milan and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2020",
abstract = "In this study, thein vivohypoglycemic effect of a donut-shaped polyanion salt (NH4)14[Na@P5W30O110]$31H2O{NaP5W30} and its Ag-containing derivative K14[Ag@P5W30O110]$22H2O$6KCl {AgP5W30}, as wellas their hepatotoxicity and nephrotoxicity, was evaluated. In the screening hypoglycemic study,Wistaralbinorats with streptozotocin induced diabetes were treated intraperitoneally with three single doses (5,10, and 20 mg per kg per b.w.) of both investigated polyoxotungstates. The blood glucose levels,measured before and after 2, 4 and 6 h polyoxotungstate application, showed that both studiedcompounds induced the most pronounced and time dependent glucose lowering effects at the doses of20 mg kg1. Thus, daily doses of 20 mg kg1were administered toWistar albinorats orally for 14 days infurther toxicity examinations. The serum glucose concentration and biochemical parameters of kidneyand liver function, as well as a histopathological analysis of kidney and liver tissues were evaluated 14days after the polyoxotungstate administration. Both investigated compounds did not induce statisticallysignificant alterations of the serum glucose and uric acid concentrations, as well as some of the liverfunction markers (serum alanine and aspartate aminotransferases, and alkaline phosphatase activities).However, the significant decrease in serum total protein and albumin concentrations and the increase inbiochemical parameters of renal function–serum urea (up to 63.1%) and creatinine concentrations (upto 23.3%) were observed for both polyoxotungstates. In addition, the detected biochemical changeswere in accordance with kidney and liver histhopathological analysis. Accordingly, the hepatotoxic andnephrotoxic effects of these potential antidiabetic polyoxotungstates could be considered as mild.",
journal = "RSC Advances",
title = "In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system",
volume = "10",
number = "5",
pages = "2846-2855",
doi = "10.1039/C9RA09790B"
}

Dinčić, M., Čolović, M. B., Sarić Matutinović, M., Ćetković, M., Kravić-Stevović, T. K., Mougharbel, A. S., Todorović, J., Ignjatović, S., Radosavljević, B., Milisavljević, M., Kortz, U.,& Krstić, D. Z.. (2020). In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system. in RSC Advances, 10(5), 2846-2855.
https://doi.org/10.1039/C9RA09790B

Dinčić M, Čolović MB, Sarić Matutinović M, Ćetković M, Kravić-Stevović TK, Mougharbel AS, Todorović J, Ignjatović S, Radosavljević B, Milisavljević M, Kortz U, Krstić DZ. In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system. in RSC Advances. 2020;10(5):2846-2855.
doi:10.1039/C9RA09790B .

Dinčić, Marko, Čolović, Mirjana B., Sarić Matutinović, Marija, Ćetković, Mila, Kravić-Stevović, Tamara K., Mougharbel, Ali S., Todorović, Jasna, Ignjatović, Svetlana, Radosavljević, Branimir, Milisavljević, Milan, Kortz, Ulrich, Krstić, Danijela Z., "In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system" in RSC Advances, 10, no. 5 (2020):2846-2855,
https://doi.org/10.1039/C9RA09790B . .

TY  - JOUR
AU  - Tošić, Jelena
AU  - Stanojević, Željka
AU  - Vidičević, Sašenka
AU  - Isaković, Aleksandra J.
AU  - Ćirić, Darko
AU  - Martinović, Tamara
AU  - Kravić-Stevović, Tamara K.
AU  - Bumbaširević, Vladimir Ž.
AU  - Paunović, Verica G.
AU  - Jovanović, Svetlana P.
AU  - Todorović-Marković, Biljana
AU  - Marković, Zoran M.
AU  - Danko, Martin
AU  - Mičušik, Matej
AU  - Spitalsky, Zdenko
AU  - Trajković, Vladimir S.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0028390818308621
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8015
AB  - We investigated the therapeutic capacity of nano-sized graphene sheets, called graphene quantum dots (GQD), in experimental autoimmune encephalomyelitis (EAE), an animal model of immune-mediated central nervous system (CNS) damage. Intraperitoneally administered GQD (10 mg/kg/day) accumulated in the lymph node and CNS cells of Dark Agouti rats in which EAE was induced by immunization with spinal cord homogenate in complete Freund's adjuvant. GQD significantly reduced clinical signs of EAE when applied throughout the course of the disease (day 0–32), while the protection was less pronounced if the treatment was limited to the induction (day 0–7 post-immunization) or effector (from day 8 onwards) phase of the disease. GQD treatment diminished immune infiltration, demyelination, axonal damage, and apoptotic death in the CNS of EAE animals. GQD also reduced the numbers of interferon-γ-expressing T helper (Th)1 cells, as well as the expression of Th1 transcription factor T-bet and proinflammatory cytokines tumor necrosis factor, interleukin-1, and granulocyte-macrophage colony-stimulating factor in the lymph nodes and CNS immune infitrates. The protective effect of GQD in EAE was associated with the activation of p38 and p42/44 mitogen-activated protein kinases (MAPK) and Akt in the lymph nodes and/or CNS. Finally, GQD protected oligodendrocytes and neurons from T cell-mediated damage in the in vitro conditions. Collectively, these data demonstrate the ability of GQD to gain access to both immune and CNS cells during neuroinflammation, and to alleviate immune-mediated CNS damage by modulating MAPK/Akt signaling and encephalitogenic Th1 immune response. © 2018 Elsevier Ltd
T2  - Neuropharmacology
T1  - Graphene quantum dots inhibit T cell-mediated neuroinflammation in rats
VL  - 146
SP  - 95
EP  - 108
DO  - 10.1016/j.neuropharm.2018.11.030
ER  - 

@article{
author = "Tošić, Jelena and Stanojević, Željka and Vidičević, Sašenka and Isaković, Aleksandra J. and Ćirić, Darko and Martinović, Tamara and Kravić-Stevović, Tamara K. and Bumbaširević, Vladimir Ž. and Paunović, Verica G. and Jovanović, Svetlana P. and Todorović-Marković, Biljana and Marković, Zoran M. and Danko, Martin and Mičušik, Matej and Spitalsky, Zdenko and Trajković, Vladimir S.",
year = "2019",
abstract = "We investigated the therapeutic capacity of nano-sized graphene sheets, called graphene quantum dots (GQD), in experimental autoimmune encephalomyelitis (EAE), an animal model of immune-mediated central nervous system (CNS) damage. Intraperitoneally administered GQD (10 mg/kg/day) accumulated in the lymph node and CNS cells of Dark Agouti rats in which EAE was induced by immunization with spinal cord homogenate in complete Freund's adjuvant. GQD significantly reduced clinical signs of EAE when applied throughout the course of the disease (day 0–32), while the protection was less pronounced if the treatment was limited to the induction (day 0–7 post-immunization) or effector (from day 8 onwards) phase of the disease. GQD treatment diminished immune infiltration, demyelination, axonal damage, and apoptotic death in the CNS of EAE animals. GQD also reduced the numbers of interferon-γ-expressing T helper (Th)1 cells, as well as the expression of Th1 transcription factor T-bet and proinflammatory cytokines tumor necrosis factor, interleukin-1, and granulocyte-macrophage colony-stimulating factor in the lymph nodes and CNS immune infitrates. The protective effect of GQD in EAE was associated with the activation of p38 and p42/44 mitogen-activated protein kinases (MAPK) and Akt in the lymph nodes and/or CNS. Finally, GQD protected oligodendrocytes and neurons from T cell-mediated damage in the in vitro conditions. Collectively, these data demonstrate the ability of GQD to gain access to both immune and CNS cells during neuroinflammation, and to alleviate immune-mediated CNS damage by modulating MAPK/Akt signaling and encephalitogenic Th1 immune response. © 2018 Elsevier Ltd",
journal = "Neuropharmacology",
title = "Graphene quantum dots inhibit T cell-mediated neuroinflammation in rats",
volume = "146",
pages = "95-108",
doi = "10.1016/j.neuropharm.2018.11.030"
}

Tošić, J., Stanojević, Ž., Vidičević, S., Isaković, A. J., Ćirić, D., Martinović, T., Kravić-Stevović, T. K., Bumbaširević, V. Ž., Paunović, V. G., Jovanović, S. P., Todorović-Marković, B., Marković, Z. M., Danko, M., Mičušik, M., Spitalsky, Z.,& Trajković, V. S.. (2019). Graphene quantum dots inhibit T cell-mediated neuroinflammation in rats. in Neuropharmacology, 146, 95-108.
https://doi.org/10.1016/j.neuropharm.2018.11.030

Tošić J, Stanojević Ž, Vidičević S, Isaković AJ, Ćirić D, Martinović T, Kravić-Stevović TK, Bumbaširević VŽ, Paunović VG, Jovanović SP, Todorović-Marković B, Marković ZM, Danko M, Mičušik M, Spitalsky Z, Trajković VS. Graphene quantum dots inhibit T cell-mediated neuroinflammation in rats. in Neuropharmacology. 2019;146:95-108.
doi:10.1016/j.neuropharm.2018.11.030 .

Tošić, Jelena, Stanojević, Željka, Vidičević, Sašenka, Isaković, Aleksandra J., Ćirić, Darko, Martinović, Tamara, Kravić-Stevović, Tamara K., Bumbaširević, Vladimir Ž., Paunović, Verica G., Jovanović, Svetlana P., Todorović-Marković, Biljana, Marković, Zoran M., Danko, Martin, Mičušik, Matej, Spitalsky, Zdenko, Trajković, Vladimir S., "Graphene quantum dots inhibit T cell-mediated neuroinflammation in rats" in Neuropharmacology, 146 (2019):95-108,
https://doi.org/10.1016/j.neuropharm.2018.11.030 . .

TY  - CONF
AU  - Ćirić, Darko
AU  - Martinović, Tamara
AU  - Kravić-Stevović, Tamara K.
AU  - Volarević, Vladislav
AU  - Paunović, Verica G.
AU  - Marković, Zoran M.
AU  - Marković-Simović, Bojana
AU  - Misirkić-Marjanović, Maja
AU  - Todorović-Marković, Biljana
AU  - Bojić, Sanja
AU  - Vučićević, Ljubica
AU  - Jovanović, Svetlana P.
AU  - Arsenijević, Nebojša N.
AU  - Holclajtner-Antunović, Ivanka D.
AU  - Milosavljević, Momir
AU  - Dramićanin, Miroslav
AU  - Lukić, Miodrag L.
AU  - Trajković, Vladimir S.
AU  - Bumbaširević, Vladimir Ž.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8741
PB  - Belgrade : Serbian Academy of Sciences and Arts
C3  - Program and Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia
T1  - Ultrastructural Analysis of Large Graphene Quantum Dots Internalization in Hepatocytes
SP  - 272
EP  - 274
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8741
ER  - 

@conference{
author = "Ćirić, Darko and Martinović, Tamara and Kravić-Stevović, Tamara K. and Volarević, Vladislav and Paunović, Verica G. and Marković, Zoran M. and Marković-Simović, Bojana and Misirkić-Marjanović, Maja and Todorović-Marković, Biljana and Bojić, Sanja and Vučićević, Ljubica and Jovanović, Svetlana P. and Arsenijević, Nebojša N. and Holclajtner-Antunović, Ivanka D. and Milosavljević, Momir and Dramićanin, Miroslav and Lukić, Miodrag L. and Trajković, Vladimir S. and Bumbaširević, Vladimir Ž.",
year = "2018",
publisher = "Belgrade : Serbian Academy of Sciences and Arts",
journal = "Program and Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia",
title = "Ultrastructural Analysis of Large Graphene Quantum Dots Internalization in Hepatocytes",
pages = "272-274",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8741"
}

Ćirić, D., Martinović, T., Kravić-Stevović, T. K., Volarević, V., Paunović, V. G., Marković, Z. M., Marković-Simović, B., Misirkić-Marjanović, M., Todorović-Marković, B., Bojić, S., Vučićević, L., Jovanović, S. P., Arsenijević, N. N., Holclajtner-Antunović, I. D., Milosavljević, M., Dramićanin, M., Lukić, M. L., Trajković, V. S.,& Bumbaširević, V. Ž.. (2018). Ultrastructural Analysis of Large Graphene Quantum Dots Internalization in Hepatocytes. in Program and Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia
Belgrade : Serbian Academy of Sciences and Arts., 272-274.
https://hdl.handle.net/21.15107/rcub_vinar_8741

Ćirić D, Martinović T, Kravić-Stevović TK, Volarević V, Paunović VG, Marković ZM, Marković-Simović B, Misirkić-Marjanović M, Todorović-Marković B, Bojić S, Vučićević L, Jovanović SP, Arsenijević NN, Holclajtner-Antunović ID, Milosavljević M, Dramićanin M, Lukić ML, Trajković VS, Bumbaširević VŽ. Ultrastructural Analysis of Large Graphene Quantum Dots Internalization in Hepatocytes. in Program and Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia. 2018;:272-274.
https://hdl.handle.net/21.15107/rcub_vinar_8741 .

Ćirić, Darko, Martinović, Tamara, Kravić-Stevović, Tamara K., Volarević, Vladislav, Paunović, Verica G., Marković, Zoran M., Marković-Simović, Bojana, Misirkić-Marjanović, Maja, Todorović-Marković, Biljana, Bojić, Sanja, Vučićević, Ljubica, Jovanović, Svetlana P., Arsenijević, Nebojša N., Holclajtner-Antunović, Ivanka D., Milosavljević, Momir, Dramićanin, Miroslav, Lukić, Miodrag L., Trajković, Vladimir S., Bumbaširević, Vladimir Ž., "Ultrastructural Analysis of Large Graphene Quantum Dots Internalization in Hepatocytes" in Program and Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia (2018):272-274,
https://hdl.handle.net/21.15107/rcub_vinar_8741 .

TY  - CONF
AU  - Kravić-Stevović, Tamara K.
AU  - Martinović, Tamara
AU  - Ćirić, Darko
AU  - Paunović, Verica G.
AU  - Ristić, Biljana
AU  - Marković, Zoran M.
AU  - Todorović-Marković, Biljana
AU  - Kosić, Milica
AU  - Prekodravac, Jovana
AU  - Micusik, Matej
AU  - Spitalsky, Zdeno
AU  - Trajković, Vladimir S.
AU  - Harhaji-Trajković, Ljubica M.
AU  - Bumbaširević, Vladimir Ž.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8740
PB  - Belgrade : Serbian Academy of Sciences and Arts
C3  - Program and Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia
T1  - Transmission Electron Microscopy in Evaluation of Curcumin Nanoparticles Cellular Uptake
SP  - 266
EP  - 268
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8740
ER  - 

@conference{
author = "Kravić-Stevović, Tamara K. and Martinović, Tamara and Ćirić, Darko and Paunović, Verica G. and Ristić, Biljana and Marković, Zoran M. and Todorović-Marković, Biljana and Kosić, Milica and Prekodravac, Jovana and Micusik, Matej and Spitalsky, Zdeno and Trajković, Vladimir S. and Harhaji-Trajković, Ljubica M. and Bumbaširević, Vladimir Ž.",
year = "2018",
publisher = "Belgrade : Serbian Academy of Sciences and Arts",
journal = "Program and Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia",
title = "Transmission Electron Microscopy in Evaluation of Curcumin Nanoparticles Cellular Uptake",
pages = "266-268",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8740"
}

Kravić-Stevović, T. K., Martinović, T., Ćirić, D., Paunović, V. G., Ristić, B., Marković, Z. M., Todorović-Marković, B., Kosić, M., Prekodravac, J., Micusik, M., Spitalsky, Z., Trajković, V. S., Harhaji-Trajković, L. M.,& Bumbaširević, V. Ž.. (2018). Transmission Electron Microscopy in Evaluation of Curcumin Nanoparticles Cellular Uptake. in Program and Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia
Belgrade : Serbian Academy of Sciences and Arts., 266-268.
https://hdl.handle.net/21.15107/rcub_vinar_8740

Kravić-Stevović TK, Martinović T, Ćirić D, Paunović VG, Ristić B, Marković ZM, Todorović-Marković B, Kosić M, Prekodravac J, Micusik M, Spitalsky Z, Trajković VS, Harhaji-Trajković LM, Bumbaširević VŽ. Transmission Electron Microscopy in Evaluation of Curcumin Nanoparticles Cellular Uptake. in Program and Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia. 2018;:266-268.
https://hdl.handle.net/21.15107/rcub_vinar_8740 .

Kravić-Stevović, Tamara K., Martinović, Tamara, Ćirić, Darko, Paunović, Verica G., Ristić, Biljana, Marković, Zoran M., Todorović-Marković, Biljana, Kosić, Milica, Prekodravac, Jovana, Micusik, Matej, Spitalsky, Zdeno, Trajković, Vladimir S., Harhaji-Trajković, Ljubica M., Bumbaširević, Vladimir Ž., "Transmission Electron Microscopy in Evaluation of Curcumin Nanoparticles Cellular Uptake" in Program and Book of Abstracts / First International Conference on Electron Microscopy of Nanostructures ELMINA 2018, August 27-29, 2018, Belgrade, Serbia (2018):266-268,
https://hdl.handle.net/21.15107/rcub_vinar_8740 .

TY  - CONF
AU  - Colic, M
AU  - Tomić, S.
AU  - Janjetović, Kristina D.
AU  - Mihajlovic, D
AU  - Milenković, M
AU  - Kravić-Stevović, Tamara K.
AU  - Marković, Zoran M.
AU  - Todorović-Marković, Biljana
AU  - Špitalsky, Zdenko
AU  - Mičušik, Matej
AU  - Vucevic, D
AU  - Trajković, Vladimir S.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7750
AB  - Graphene quantum dots (GQD) are atom-thick nanodimensional carbon, very attractive for the application in theranostics due to their excellent physico-chemical and biological properties. However, their immunoregulatory properties are insufficiently investigated, especially for human immune cells. We found that non-toxic doses of GQD inhibit the production of proinflammatory and T helper (Th)1 cytokines, and augment the production of anti-inflammatory and Th2 cytokines by human peripheral blood  mononuclear cells. While unable to affect purified T cells directly, GQD impaired the differentiation and functions of monocyte-derived dendritic cells (DC), lowering  their capacity to stimulate T cell proliferation, development of Th1 and Th17 cells, and T-cell mediated cytotoxicity. Additionally, GQD-treated DC potentiated Th2  polarization, and induced suppressive CD4+CD25hiFoxp3hi regulatory T cells. After internalization in a dynamin-independent, cholesterol-dependent manner, GQD lowered the ROS generation and nuclear translocation of NF-B in DC. The activity of mammalian target of rapamycin (mTOR) was reduced by GQD, which correlated with the increase in transcription of autophagy genes and autophagic flux in DC. Genetic suppression of autophagy impaired the pro-tolerogenic effects of GQD on DC. Our results suggest that GQD-triggered autophagy promotes tolerogenic functions in monocyte-derived DC, which could be beneficial in inflammatory T-cell mediated pathologies, but also harmful in GQD-based anti-cancer therapy.
C3  - European Journal of Immunology
T1  - Graphene quantum dots induce tolerogenic properties in dendritic cells via induction of autophagy
VL  - 48
IS  - Supplement 1
SP  - 180
EP  - 181
DO  - 10.1002/eji.201871000
ER  - 

@conference{
author = "Colic, M and Tomić, S. and Janjetović, Kristina D. and Mihajlovic, D and Milenković, M and Kravić-Stevović, Tamara K. and Marković, Zoran M. and Todorović-Marković, Biljana and Špitalsky, Zdenko and Mičušik, Matej and Vucevic, D and Trajković, Vladimir S.",
year = "2018",
abstract = "Graphene quantum dots (GQD) are atom-thick nanodimensional carbon, very attractive for the application in theranostics due to their excellent physico-chemical and biological properties. However, their immunoregulatory properties are insufficiently investigated, especially for human immune cells. We found that non-toxic doses of GQD inhibit the production of proinflammatory and T helper (Th)1 cytokines, and augment the production of anti-inflammatory and Th2 cytokines by human peripheral blood  mononuclear cells. While unable to affect purified T cells directly, GQD impaired the differentiation and functions of monocyte-derived dendritic cells (DC), lowering  their capacity to stimulate T cell proliferation, development of Th1 and Th17 cells, and T-cell mediated cytotoxicity. Additionally, GQD-treated DC potentiated Th2  polarization, and induced suppressive CD4+CD25hiFoxp3hi regulatory T cells. After internalization in a dynamin-independent, cholesterol-dependent manner, GQD lowered the ROS generation and nuclear translocation of NF-B in DC. The activity of mammalian target of rapamycin (mTOR) was reduced by GQD, which correlated with the increase in transcription of autophagy genes and autophagic flux in DC. Genetic suppression of autophagy impaired the pro-tolerogenic effects of GQD on DC. Our results suggest that GQD-triggered autophagy promotes tolerogenic functions in monocyte-derived DC, which could be beneficial in inflammatory T-cell mediated pathologies, but also harmful in GQD-based anti-cancer therapy.",
journal = "European Journal of Immunology",
title = "Graphene quantum dots induce tolerogenic properties in dendritic cells via induction of autophagy",
volume = "48",
number = "Supplement 1",
pages = "180-181",
doi = "10.1002/eji.201871000"
}

Colic, M., Tomić, S., Janjetović, K. D., Mihajlovic, D., Milenković, M., Kravić-Stevović, T. K., Marković, Z. M., Todorović-Marković, B., Špitalsky, Z., Mičušik, M., Vucevic, D.,& Trajković, V. S.. (2018). Graphene quantum dots induce tolerogenic properties in dendritic cells via induction of autophagy. in European Journal of Immunology, 48(Supplement 1), 180-181.
https://doi.org/10.1002/eji.201871000

Colic M, Tomić S, Janjetović KD, Mihajlovic D, Milenković M, Kravić-Stevović TK, Marković ZM, Todorović-Marković B, Špitalsky Z, Mičušik M, Vucevic D, Trajković VS. Graphene quantum dots induce tolerogenic properties in dendritic cells via induction of autophagy. in European Journal of Immunology. 2018;48(Supplement 1):180-181.
doi:10.1002/eji.201871000 .

Colic, M, Tomić, S., Janjetović, Kristina D., Mihajlovic, D, Milenković, M, Kravić-Stevović, Tamara K., Marković, Zoran M., Todorović-Marković, Biljana, Špitalsky, Zdenko, Mičušik, Matej, Vucevic, D, Trajković, Vladimir S., "Graphene quantum dots induce tolerogenic properties in dendritic cells via induction of autophagy" in European Journal of Immunology, 48, no. Supplement 1 (2018):180-181,
https://doi.org/10.1002/eji.201871000 . .

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Medic, Branislava
AU  - Cetkovic, Mila
AU  - Kravić-Stevović, Tamara K.
AU  - Stojanović, Marko
AU  - Ayass, Wassim W.
AU  - Mougharbel, Ali S.
AU  - Radenković, Miroslav
AU  - Prostran, Milica
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1748
AB  - A toxicity evaluation of two Keggin-type heteropolytungstates, K-7[Ti2PW10O40].6H(2)O and K6H [SiV3W9O40].3H(2)O, with different inhibitory potencies toward acetylcholinesterase activity (IC50 values of 1.04 x 10(-6) and 4.80 x 10(-4) mol/L, respectively) was performed. Wistar albino rats were orally treated with single doses (5 and 50 mg/kg) of both investigated compounds. The biochemical parameters of renal (serum urea and creatinine) and liver function (direct and total bilirubin, alanine transaminase, and aspartate aminotransferase) were determined after 24 h and 14 days. A histopathological analysis of liver tissue was carried out 14 days after the polyoxotungstate administration. Both applied doses of the investigated compounds did not induce statistically significant alterations of the renal function markers. However, the polyoxotungstate treatment caused an increase in the activities of serum alanine transaminase and aspartate aminotransferase in a time- and concentration -dependent manner, although statistically significant changes in bilirubin concentrations were not observed. Furthermore, the detected hepatotoxic effect was confirmed by histhopathological analysis that suggested some reversible liver tissue damage two weeks after the treatment, especially in the case of K6H [SiV3W9O40]-3H(2)O. Accordingly, the toxicity of these two polyoxotungstates with anti-acetylcholinesterase effect cannot be considered as a severe one, but their potential clinical application would require a more complex toxicological study.
T2  - Toxicology and Applied Pharmacology
T1  - Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity
VL  - 333
SP  - 68
EP  - 75
DO  - 10.1016/j.taap.2017.08.010
ER  - 

@article{
author = "Čolović, Mirjana B. and Medic, Branislava and Cetkovic, Mila and Kravić-Stevović, Tamara K. and Stojanović, Marko and Ayass, Wassim W. and Mougharbel, Ali S. and Radenković, Miroslav and Prostran, Milica and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2017",
abstract = "A toxicity evaluation of two Keggin-type heteropolytungstates, K-7[Ti2PW10O40].6H(2)O and K6H [SiV3W9O40].3H(2)O, with different inhibitory potencies toward acetylcholinesterase activity (IC50 values of 1.04 x 10(-6) and 4.80 x 10(-4) mol/L, respectively) was performed. Wistar albino rats were orally treated with single doses (5 and 50 mg/kg) of both investigated compounds. The biochemical parameters of renal (serum urea and creatinine) and liver function (direct and total bilirubin, alanine transaminase, and aspartate aminotransferase) were determined after 24 h and 14 days. A histopathological analysis of liver tissue was carried out 14 days after the polyoxotungstate administration. Both applied doses of the investigated compounds did not induce statistically significant alterations of the renal function markers. However, the polyoxotungstate treatment caused an increase in the activities of serum alanine transaminase and aspartate aminotransferase in a time- and concentration -dependent manner, although statistically significant changes in bilirubin concentrations were not observed. Furthermore, the detected hepatotoxic effect was confirmed by histhopathological analysis that suggested some reversible liver tissue damage two weeks after the treatment, especially in the case of K6H [SiV3W9O40]-3H(2)O. Accordingly, the toxicity of these two polyoxotungstates with anti-acetylcholinesterase effect cannot be considered as a severe one, but their potential clinical application would require a more complex toxicological study.",
journal = "Toxicology and Applied Pharmacology",
title = "Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity",
volume = "333",
pages = "68-75",
doi = "10.1016/j.taap.2017.08.010"
}

Čolović, M. B., Medic, B., Cetkovic, M., Kravić-Stevović, T. K., Stojanović, M., Ayass, W. W., Mougharbel, A. S., Radenković, M., Prostran, M., Kortz, U.,& Krstić, D. Z.. (2017). Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity. in Toxicology and Applied Pharmacology, 333, 68-75.
https://doi.org/10.1016/j.taap.2017.08.010

Čolović MB, Medic B, Cetkovic M, Kravić-Stevović TK, Stojanović M, Ayass WW, Mougharbel AS, Radenković M, Prostran M, Kortz U, Krstić DZ. Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity. in Toxicology and Applied Pharmacology. 2017;333:68-75.
doi:10.1016/j.taap.2017.08.010 .

Čolović, Mirjana B., Medic, Branislava, Cetkovic, Mila, Kravić-Stevović, Tamara K., Stojanović, Marko, Ayass, Wassim W., Mougharbel, Ali S., Radenković, Miroslav, Prostran, Milica, Kortz, Ulrich, Krstić, Danijela Z., "Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity" in Toxicology and Applied Pharmacology, 333 (2017):68-75,
https://doi.org/10.1016/j.taap.2017.08.010 . .

TY  - JOUR
AU  - Tomić, Sergej
AU  - Janjetović, Kristina D.
AU  - Mihajlovic, Dusan
AU  - Milenković, Marina
AU  - Kravić-Stevović, Tamara K.
AU  - Marković, Zoran M.
AU  - Todorović-Marković, Biljana
AU  - Špitalsky, Zdenko
AU  - Mičušik, Matej
AU  - Vucevic, Dragana
AU  - Colic, Miodrag
AU  - Trajković, Vladimir S.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1782
AB  - Graphene quantum dots (GQD) are atom-thick nanodimensional carbon sheets with excellent physicochemical and biological properties; making them attractive for application in theranostics: However, their immunoregulatory properties are insufficiently investigated, especially in human primary immune cells. We found that non-toxic doses of GQD inhibit the production of proinflammatory and T helper (Th) 1 cytokines, and augment the production of anti-inflammatory and Th2 cytokines by human peripheral blood mononuclear cells. While unable to affect T cells directly, GQD impaired the differentiation and functions of monocyte-derived dendritic cells (DC), lowering their capacity to stimulate T cell proliferation, development of Thl and Th17 cells, and T-cell mediated cytotoxicity. Additionally, GQD-treated DC potentiated Th2 polarization, and induced suppressive CD4(+)CD25(high)Foxp3(+) regulatory T cells. After internalization in a dynamin-independent, cholesterol-dependent manner, GQD lowered the production of reactive oxygen species and nuclear translocation of NF-kappa B in DC. The activity of mammalian target of rapamycin (mTOR) was reduced by GQD, which correlated with the increase in transcription of autophagy genes and autophagic flux in DC. Genetic suppression of autophagy impaired the pro-tolerogenic effects of GQD on DC. Our results suggest that GQD-triggered autophagy promotes tolerogenic functions in monocyte-derived DC, which could be beneficial in inflammatory T-cell mediated pathologies, but also harmful in GQD-based anti-cancer therapy. (C) 2017 Elsevier Ltd. All rights reserved.
T2  - Biomaterials
T1  - Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells
VL  - 146
SP  - 13
EP  - 28
DO  - 10.1016/j.biomaterials.2017.08.040
ER  - 

@article{
author = "Tomić, Sergej and Janjetović, Kristina D. and Mihajlovic, Dusan and Milenković, Marina and Kravić-Stevović, Tamara K. and Marković, Zoran M. and Todorović-Marković, Biljana and Špitalsky, Zdenko and Mičušik, Matej and Vucevic, Dragana and Colic, Miodrag and Trajković, Vladimir S.",
year = "2017",
abstract = "Graphene quantum dots (GQD) are atom-thick nanodimensional carbon sheets with excellent physicochemical and biological properties; making them attractive for application in theranostics: However, their immunoregulatory properties are insufficiently investigated, especially in human primary immune cells. We found that non-toxic doses of GQD inhibit the production of proinflammatory and T helper (Th) 1 cytokines, and augment the production of anti-inflammatory and Th2 cytokines by human peripheral blood mononuclear cells. While unable to affect T cells directly, GQD impaired the differentiation and functions of monocyte-derived dendritic cells (DC), lowering their capacity to stimulate T cell proliferation, development of Thl and Th17 cells, and T-cell mediated cytotoxicity. Additionally, GQD-treated DC potentiated Th2 polarization, and induced suppressive CD4(+)CD25(high)Foxp3(+) regulatory T cells. After internalization in a dynamin-independent, cholesterol-dependent manner, GQD lowered the production of reactive oxygen species and nuclear translocation of NF-kappa B in DC. The activity of mammalian target of rapamycin (mTOR) was reduced by GQD, which correlated with the increase in transcription of autophagy genes and autophagic flux in DC. Genetic suppression of autophagy impaired the pro-tolerogenic effects of GQD on DC. Our results suggest that GQD-triggered autophagy promotes tolerogenic functions in monocyte-derived DC, which could be beneficial in inflammatory T-cell mediated pathologies, but also harmful in GQD-based anti-cancer therapy. (C) 2017 Elsevier Ltd. All rights reserved.",
journal = "Biomaterials",
title = "Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells",
volume = "146",
pages = "13-28",
doi = "10.1016/j.biomaterials.2017.08.040"
}

Tomić, S., Janjetović, K. D., Mihajlovic, D., Milenković, M., Kravić-Stevović, T. K., Marković, Z. M., Todorović-Marković, B., Špitalsky, Z., Mičušik, M., Vucevic, D., Colic, M.,& Trajković, V. S.. (2017). Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells. in Biomaterials, 146, 13-28.
https://doi.org/10.1016/j.biomaterials.2017.08.040

Tomić S, Janjetović KD, Mihajlovic D, Milenković M, Kravić-Stevović TK, Marković ZM, Todorović-Marković B, Špitalsky Z, Mičušik M, Vucevic D, Colic M, Trajković VS. Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells. in Biomaterials. 2017;146:13-28.
doi:10.1016/j.biomaterials.2017.08.040 .

Tomić, Sergej, Janjetović, Kristina D., Mihajlovic, Dusan, Milenković, Marina, Kravić-Stevović, Tamara K., Marković, Zoran M., Todorović-Marković, Biljana, Špitalsky, Zdenko, Mičušik, Matej, Vucevic, Dragana, Colic, Miodrag, Trajković, Vladimir S., "Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells" in Biomaterials, 146 (2017):13-28,
https://doi.org/10.1016/j.biomaterials.2017.08.040 . .

TY  - JOUR
AU  - Paunović, Verica G.
AU  - Ristić, Biljana
AU  - Marković, Zoran M.
AU  - Todorović-Marković, Biljana
AU  - Kosić, Milica
AU  - Prekodravac, Jovana
AU  - Kravić-Stevović, Tamara K.
AU  - Martinović, Tamara
AU  - Mičušik, Matej
AU  - Špitalsky, Zdenko
AU  - Trajković, Vladimir S.
AU  - Harhaji-Trajković, Ljubica M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1084
AB  - Indian spice curcumin is known for its anticancer properties, but the anticancer mechanisms of nanoparticulate curcumin have not been completely elucidated. We here investigated the in vitro anticancer effect of blue light (470 nm, 1 W)-irradiated curcumin nanoparticles prepared by tetrahydrofuran/water solvent exchange, using U251 glioma, B16 melanoma, and H460 lung cancer cells as targets. The size of curcumin nanocrystals was approximately 250 nm, while photoexcitation induced their oxidation and partial agglomeration. Although cell membrane in the absence of light was almost impermeable to curcumin nanoparticles, photoexcitation stimulated their internalization. While irradiation with blue light (1-8 min) or nanocurcumin (1.25-10 mu g/ml) alone was only marginally toxic to tumor cells, photoexcited nanocurcumin displayed a significant cytotoxicity depending both on the irradiation time and nanocurcumin concentration. Photoexcited nanocurcumin induced phosphorylation of cJun N-terminal kinase (JNK), mitochondrial depolarization, caspase-3 activation, and cleavage of poly (ADP-ribose) polymerase, indicating apoptotic cell death. Accordingly, pharmacologial inhibition of JNK and caspase activity rescued cancer cells from photoexcited nanocurcumin. On the other hand, antioxidant treatment did not reduce photocytotoxicity of nanocurcumin, arguing against the involvement of oxidative stress. By demonstrating the ability of photoexcited nanocurcumin to induce oxidative-stress independent, JNK-and caspase-dependent apoptosis, our results support its further investigation in cancer therapy.
PB  - Springer
T2  - Biomedical Microdevices
T1  - c-Jun N-terminal kinase-dependent apoptotic photocytotoxicity of solvent exchange-prepared curcumin nanoparticles
VL  - 18
IS  - 2
DO  - 10.1007/s10544-016-0062-2
ER  - 

@article{
author = "Paunović, Verica G. and Ristić, Biljana and Marković, Zoran M. and Todorović-Marković, Biljana and Kosić, Milica and Prekodravac, Jovana and Kravić-Stevović, Tamara K. and Martinović, Tamara and Mičušik, Matej and Špitalsky, Zdenko and Trajković, Vladimir S. and Harhaji-Trajković, Ljubica M.",
year = "2016",
abstract = "Indian spice curcumin is known for its anticancer properties, but the anticancer mechanisms of nanoparticulate curcumin have not been completely elucidated. We here investigated the in vitro anticancer effect of blue light (470 nm, 1 W)-irradiated curcumin nanoparticles prepared by tetrahydrofuran/water solvent exchange, using U251 glioma, B16 melanoma, and H460 lung cancer cells as targets. The size of curcumin nanocrystals was approximately 250 nm, while photoexcitation induced their oxidation and partial agglomeration. Although cell membrane in the absence of light was almost impermeable to curcumin nanoparticles, photoexcitation stimulated their internalization. While irradiation with blue light (1-8 min) or nanocurcumin (1.25-10 mu g/ml) alone was only marginally toxic to tumor cells, photoexcited nanocurcumin displayed a significant cytotoxicity depending both on the irradiation time and nanocurcumin concentration. Photoexcited nanocurcumin induced phosphorylation of cJun N-terminal kinase (JNK), mitochondrial depolarization, caspase-3 activation, and cleavage of poly (ADP-ribose) polymerase, indicating apoptotic cell death. Accordingly, pharmacologial inhibition of JNK and caspase activity rescued cancer cells from photoexcited nanocurcumin. On the other hand, antioxidant treatment did not reduce photocytotoxicity of nanocurcumin, arguing against the involvement of oxidative stress. By demonstrating the ability of photoexcited nanocurcumin to induce oxidative-stress independent, JNK-and caspase-dependent apoptosis, our results support its further investigation in cancer therapy.",
publisher = "Springer",
journal = "Biomedical Microdevices",
title = "c-Jun N-terminal kinase-dependent apoptotic photocytotoxicity of solvent exchange-prepared curcumin nanoparticles",
volume = "18",
number = "2",
doi = "10.1007/s10544-016-0062-2"
}

Paunović, V. G., Ristić, B., Marković, Z. M., Todorović-Marković, B., Kosić, M., Prekodravac, J., Kravić-Stevović, T. K., Martinović, T., Mičušik, M., Špitalsky, Z., Trajković, V. S.,& Harhaji-Trajković, L. M.. (2016). c-Jun N-terminal kinase-dependent apoptotic photocytotoxicity of solvent exchange-prepared curcumin nanoparticles. in Biomedical Microdevices
Springer., 18(2).
https://doi.org/10.1007/s10544-016-0062-2

Paunović VG, Ristić B, Marković ZM, Todorović-Marković B, Kosić M, Prekodravac J, Kravić-Stevović TK, Martinović T, Mičušik M, Špitalsky Z, Trajković VS, Harhaji-Trajković LM. c-Jun N-terminal kinase-dependent apoptotic photocytotoxicity of solvent exchange-prepared curcumin nanoparticles. in Biomedical Microdevices. 2016;18(2).
doi:10.1007/s10544-016-0062-2 .

Paunović, Verica G., Ristić, Biljana, Marković, Zoran M., Todorović-Marković, Biljana, Kosić, Milica, Prekodravac, Jovana, Kravić-Stevović, Tamara K., Martinović, Tamara, Mičušik, Matej, Špitalsky, Zdenko, Trajković, Vladimir S., Harhaji-Trajković, Ljubica M., "c-Jun N-terminal kinase-dependent apoptotic photocytotoxicity of solvent exchange-prepared curcumin nanoparticles" in Biomedical Microdevices, 18, no. 2 (2016),
https://doi.org/10.1007/s10544-016-0062-2 . .

TY  - CONF
AU  - Tošić, Jelena
AU  - Vidičević, Sašenka
AU  - Stanojević, Željka
AU  - Paunović, Verica G.
AU  - Petricevic, S.
AU  - Martinović, Tamara
AU  - Kravić-Stevović, Tamara K.
AU  - Ćirić, Darko
AU  - Marković, Zoran M.
AU  - Isaković, Aleksandra J.
AU  - Trajković, Vladimir S.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7160
C3  - European Neuropsychopharmacology
T1  - Graphene quantum dots show protective effect on a model of experimental autoimmune encephalomyelitis
VL  - 26
SP  - S211
EP  - S212
DO  - 10.1016/S0924-977X(16)31060-4
ER  - 

@conference{
author = "Tošić, Jelena and Vidičević, Sašenka and Stanojević, Željka and Paunović, Verica G. and Petricevic, S. and Martinović, Tamara and Kravić-Stevović, Tamara K. and Ćirić, Darko and Marković, Zoran M. and Isaković, Aleksandra J. and Trajković, Vladimir S.",
year = "2016",
journal = "European Neuropsychopharmacology",
title = "Graphene quantum dots show protective effect on a model of experimental autoimmune encephalomyelitis",
volume = "26",
pages = "S211-S212",
doi = "10.1016/S0924-977X(16)31060-4"
}

Tošić, J., Vidičević, S., Stanojević, Ž., Paunović, V. G., Petricevic, S., Martinović, T., Kravić-Stevović, T. K., Ćirić, D., Marković, Z. M., Isaković, A. J.,& Trajković, V. S.. (2016). Graphene quantum dots show protective effect on a model of experimental autoimmune encephalomyelitis. in European Neuropsychopharmacology, 26, S211-S212.
https://doi.org/10.1016/S0924-977X(16)31060-4

Tošić J, Vidičević S, Stanojević Ž, Paunović VG, Petricevic S, Martinović T, Kravić-Stevović TK, Ćirić D, Marković ZM, Isaković AJ, Trajković VS. Graphene quantum dots show protective effect on a model of experimental autoimmune encephalomyelitis. in European Neuropsychopharmacology. 2016;26:S211-S212.
doi:10.1016/S0924-977X(16)31060-4 .

Tošić, Jelena, Vidičević, Sašenka, Stanojević, Željka, Paunović, Verica G., Petricevic, S., Martinović, Tamara, Kravić-Stevović, Tamara K., Ćirić, Darko, Marković, Zoran M., Isaković, Aleksandra J., Trajković, Vladimir S., "Graphene quantum dots show protective effect on a model of experimental autoimmune encephalomyelitis" in European Neuropsychopharmacology, 26 (2016):S211-S212,
https://doi.org/10.1016/S0924-977X(16)31060-4 . .

TY  - CONF
AU  - Volarevic, V.
AU  - Paunović, Verica G.
AU  - Marković, Zoran M.
AU  - Marković-Simović, Bojana
AU  - Misirkić-Marjanović, Maja
AU  - Todorović-Marković, Biljana
AU  - Bojic, S.
AU  - Vucicevic, L.
AU  - Jovanović, Svetlana P.
AU  - Arsenijević, Nebojša N.
AU  - Holclajtner-Antunović, Ivanka D.
AU  - Milosavljević, Momir
AU  - Dramićanin, Miroslav
AU  - Kravić-Stevović, Tamara K.
AU  - Ćirić, Darko
AU  - Lukić, M. L.
AU  - Trajković, Vladimir S.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7082
C3  - Journal of Hepatology
T1  - Graphene Quantum Dots Attenuate Concanavalin A-Induced Hepatitis
VL  - 62
SP  - S483
EP  - S484
DO  - 10.1016/S0168-8278(15)30665-6
ER  - 

@conference{
author = "Volarevic, V. and Paunović, Verica G. and Marković, Zoran M. and Marković-Simović, Bojana and Misirkić-Marjanović, Maja and Todorović-Marković, Biljana and Bojic, S. and Vucicevic, L. and Jovanović, Svetlana P. and Arsenijević, Nebojša N. and Holclajtner-Antunović, Ivanka D. and Milosavljević, Momir and Dramićanin, Miroslav and Kravić-Stevović, Tamara K. and Ćirić, Darko and Lukić, M. L. and Trajković, Vladimir S.",
year = "2015",
journal = "Journal of Hepatology",
title = "Graphene Quantum Dots Attenuate Concanavalin A-Induced Hepatitis",
volume = "62",
pages = "S483-S484",
doi = "10.1016/S0168-8278(15)30665-6"
}

Volarevic, V., Paunović, V. G., Marković, Z. M., Marković-Simović, B., Misirkić-Marjanović, M., Todorović-Marković, B., Bojic, S., Vucicevic, L., Jovanović, S. P., Arsenijević, N. N., Holclajtner-Antunović, I. D., Milosavljević, M., Dramićanin, M., Kravić-Stevović, T. K., Ćirić, D., Lukić, M. L.,& Trajković, V. S.. (2015). Graphene Quantum Dots Attenuate Concanavalin A-Induced Hepatitis. in Journal of Hepatology, 62, S483-S484.
https://doi.org/10.1016/S0168-8278(15)30665-6

Volarevic V, Paunović VG, Marković ZM, Marković-Simović B, Misirkić-Marjanović M, Todorović-Marković B, Bojic S, Vucicevic L, Jovanović SP, Arsenijević NN, Holclajtner-Antunović ID, Milosavljević M, Dramićanin M, Kravić-Stevović TK, Ćirić D, Lukić ML, Trajković VS. Graphene Quantum Dots Attenuate Concanavalin A-Induced Hepatitis. in Journal of Hepatology. 2015;62:S483-S484.
doi:10.1016/S0168-8278(15)30665-6 .

Volarevic, V., Paunović, Verica G., Marković, Zoran M., Marković-Simović, Bojana, Misirkić-Marjanović, Maja, Todorović-Marković, Biljana, Bojic, S., Vucicevic, L., Jovanović, Svetlana P., Arsenijević, Nebojša N., Holclajtner-Antunović, Ivanka D., Milosavljević, Momir, Dramićanin, Miroslav, Kravić-Stevović, Tamara K., Ćirić, Darko, Lukić, M. L., Trajković, Vladimir S., "Graphene Quantum Dots Attenuate Concanavalin A-Induced Hepatitis" in Journal of Hepatology, 62 (2015):S483-S484,
https://doi.org/10.1016/S0168-8278(15)30665-6 . .

TY  - JOUR
AU  - Volarevic, Vladislav
AU  - Paunović, Verica G.
AU  - Marković, Zoran M.
AU  - Marković-Simović, Bojana
AU  - Misirkić-Marjanović, Maja
AU  - Todorović-Marković, Biljana
AU  - Bojic, Sanja
AU  - Vucicevic, Ljubica
AU  - Jovanović, Svetlana P.
AU  - Arsenijević, Nebojša N.
AU  - Holclajtner-Antunović, Ivanka D.
AU  - Milosavljević, Momir
AU  - Dramićanin, Miroslav
AU  - Kravić-Stevović, Tamara K.
AU  - Ćirić, Darko
AU  - Lukić, Miodrag L.
AU  - Trajković, Vladimir S.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/324
AB  - We investigated the effect of large (40 nm) graphene quantum dots (GQDs) in concanavalin A (Con A; 12 mg/kg i.v.)-induced mouse hepatitis, a T cell-mediated liver injury resembling fulminant hepatitis in humans. Intravenously injected GQDs (50 mg/kg) accumulated in liver and reduced Con A-mediated liver damage, as demonstrated by histopathological analysis and a decrease in liver lipid peroxidation and serum levels of liver transaminases. The cleavage of apoptotic markers caspase-3/PARP and mRNA levels of proapoptotic mediators Puma, Noxa, Bax, Bak1, Bim, Apaf1, and p21, as well as LC3-I conversion to autophagosome-associated LC3-II and expression of autophagy-related (Atg) genes Atg4b, Atg7, Atg12, and beclin-1, were attenuated by GQDs, indicating a decrease in both apoptosis and autophagy in the liver tissue. This was associated with the reduced liver infiltration of immune cells, particularly the T cells producing proinflammatory cytokine IFN-?, and a decrease in IFN-gamma serum levels. In the spleen of GQD-exposed mice, mRNA expression of IFN-? and its transcription factor T-bet was reduced, while that of the IL-33 ligand ST2 was increased. The hepatoprotective effect of GQDs was less pronounced in ST2-deficient mice, indicating that it might depend on ST2 upregulation. In vitro, GQDs inhibited splenocyte IFN-gamma production, reduced the activation of extracellular signal-regulated kinase in macrophage and T cell lines, inhibited macrophage production of the free radical nitric oxide, and reduced its cytotoxicity toward hepatocyte cell line HepG2. Therefore, GQDs alleviate immune-mediated fulminant hepatitis by interfering with T cell and macrophage activation and possibly by exerting a direct hepatoprotective effect.
T2  - ACS Nano
T1  - Large Graphene Quantum Dots Alleviate Immune-Mediated Liver Damage
VL  - 8
IS  - 12
SP  - 12098
EP  - 12109
DO  - 10.1021/nn502466z
ER  - 

@article{
author = "Volarevic, Vladislav and Paunović, Verica G. and Marković, Zoran M. and Marković-Simović, Bojana and Misirkić-Marjanović, Maja and Todorović-Marković, Biljana and Bojic, Sanja and Vucicevic, Ljubica and Jovanović, Svetlana P. and Arsenijević, Nebojša N. and Holclajtner-Antunović, Ivanka D. and Milosavljević, Momir and Dramićanin, Miroslav and Kravić-Stevović, Tamara K. and Ćirić, Darko and Lukić, Miodrag L. and Trajković, Vladimir S.",
year = "2014",
abstract = "We investigated the effect of large (40 nm) graphene quantum dots (GQDs) in concanavalin A (Con A; 12 mg/kg i.v.)-induced mouse hepatitis, a T cell-mediated liver injury resembling fulminant hepatitis in humans. Intravenously injected GQDs (50 mg/kg) accumulated in liver and reduced Con A-mediated liver damage, as demonstrated by histopathological analysis and a decrease in liver lipid peroxidation and serum levels of liver transaminases. The cleavage of apoptotic markers caspase-3/PARP and mRNA levels of proapoptotic mediators Puma, Noxa, Bax, Bak1, Bim, Apaf1, and p21, as well as LC3-I conversion to autophagosome-associated LC3-II and expression of autophagy-related (Atg) genes Atg4b, Atg7, Atg12, and beclin-1, were attenuated by GQDs, indicating a decrease in both apoptosis and autophagy in the liver tissue. This was associated with the reduced liver infiltration of immune cells, particularly the T cells producing proinflammatory cytokine IFN-?, and a decrease in IFN-gamma serum levels. In the spleen of GQD-exposed mice, mRNA expression of IFN-? and its transcription factor T-bet was reduced, while that of the IL-33 ligand ST2 was increased. The hepatoprotective effect of GQDs was less pronounced in ST2-deficient mice, indicating that it might depend on ST2 upregulation. In vitro, GQDs inhibited splenocyte IFN-gamma production, reduced the activation of extracellular signal-regulated kinase in macrophage and T cell lines, inhibited macrophage production of the free radical nitric oxide, and reduced its cytotoxicity toward hepatocyte cell line HepG2. Therefore, GQDs alleviate immune-mediated fulminant hepatitis by interfering with T cell and macrophage activation and possibly by exerting a direct hepatoprotective effect.",
journal = "ACS Nano",
title = "Large Graphene Quantum Dots Alleviate Immune-Mediated Liver Damage",
volume = "8",
number = "12",
pages = "12098-12109",
doi = "10.1021/nn502466z"
}

Volarevic, V., Paunović, V. G., Marković, Z. M., Marković-Simović, B., Misirkić-Marjanović, M., Todorović-Marković, B., Bojic, S., Vucicevic, L., Jovanović, S. P., Arsenijević, N. N., Holclajtner-Antunović, I. D., Milosavljević, M., Dramićanin, M., Kravić-Stevović, T. K., Ćirić, D., Lukić, M. L.,& Trajković, V. S.. (2014). Large Graphene Quantum Dots Alleviate Immune-Mediated Liver Damage. in ACS Nano, 8(12), 12098-12109.
https://doi.org/10.1021/nn502466z

Volarevic V, Paunović VG, Marković ZM, Marković-Simović B, Misirkić-Marjanović M, Todorović-Marković B, Bojic S, Vucicevic L, Jovanović SP, Arsenijević NN, Holclajtner-Antunović ID, Milosavljević M, Dramićanin M, Kravić-Stevović TK, Ćirić D, Lukić ML, Trajković VS. Large Graphene Quantum Dots Alleviate Immune-Mediated Liver Damage. in ACS Nano. 2014;8(12):12098-12109.
doi:10.1021/nn502466z .

Volarevic, Vladislav, Paunović, Verica G., Marković, Zoran M., Marković-Simović, Bojana, Misirkić-Marjanović, Maja, Todorović-Marković, Biljana, Bojic, Sanja, Vucicevic, Ljubica, Jovanović, Svetlana P., Arsenijević, Nebojša N., Holclajtner-Antunović, Ivanka D., Milosavljević, Momir, Dramićanin, Miroslav, Kravić-Stevović, Tamara K., Ćirić, Darko, Lukić, Miodrag L., Trajković, Vladimir S., "Large Graphene Quantum Dots Alleviate Immune-Mediated Liver Damage" in ACS Nano, 8, no. 12 (2014):12098-12109,
https://doi.org/10.1021/nn502466z . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Ristić, Biljana Z.
AU  - Arsikin, Katarina M.
AU  - Klisic, Djordje G.
AU  - Harhaji-Trajković, Ljubica M.
AU  - Todorović-Marković, Biljana
AU  - Kepić, Dejan P.
AU  - Kravić-Stevović, Tamara K.
AU  - Jovanović, Svetlana P.
AU  - Milenković, Marina
AU  - Milivojević, Dušan
AU  - Bumbaširević, Vladimir Ž.
AU  - Dramićanin, Miroslav
AU  - Trajković, Vladimir S.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5025
AB  - The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470 nm, 1 W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. The cell death induced by photoexcited GQD displayed morphological and/or biochemical characteristics of both apoptosis (phosphatidylserine externalization, caspase activation. DNA fragmentation) and autophagy (formation of autophagic vesicles, LC3-I/LC3-II conversion, degradation of autophagic target p62). Moreover, a genetic inactivation of autophagy-essential LOB protein partly abrogated the photodynamic cytotoxicity of GQD. These data indicate potential usefulness of GQD in photodynamic therapy, but also raise concerns about their possible toxicity. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Biomaterials
T1  - Graphene quantum dots as autophagy-inducing photodynamic agents
VL  - 33
IS  - 29
SP  - 7084
EP  - 7092
DO  - 10.1016/j.biomaterials.2012.06.060
ER  - 

@article{
author = "Marković, Zoran M. and Ristić, Biljana Z. and Arsikin, Katarina M. and Klisic, Djordje G. and Harhaji-Trajković, Ljubica M. and Todorović-Marković, Biljana and Kepić, Dejan P. and Kravić-Stevović, Tamara K. and Jovanović, Svetlana P. and Milenković, Marina and Milivojević, Dušan and Bumbaširević, Vladimir Ž. and Dramićanin, Miroslav and Trajković, Vladimir S.",
year = "2012",
abstract = "The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470 nm, 1 W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. The cell death induced by photoexcited GQD displayed morphological and/or biochemical characteristics of both apoptosis (phosphatidylserine externalization, caspase activation. DNA fragmentation) and autophagy (formation of autophagic vesicles, LC3-I/LC3-II conversion, degradation of autophagic target p62). Moreover, a genetic inactivation of autophagy-essential LOB protein partly abrogated the photodynamic cytotoxicity of GQD. These data indicate potential usefulness of GQD in photodynamic therapy, but also raise concerns about their possible toxicity. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Biomaterials",
title = "Graphene quantum dots as autophagy-inducing photodynamic agents",
volume = "33",
number = "29",
pages = "7084-7092",
doi = "10.1016/j.biomaterials.2012.06.060"
}

Marković, Z. M., Ristić, B. Z., Arsikin, K. M., Klisic, D. G., Harhaji-Trajković, L. M., Todorović-Marković, B., Kepić, D. P., Kravić-Stevović, T. K., Jovanović, S. P., Milenković, M., Milivojević, D., Bumbaširević, V. Ž., Dramićanin, M.,& Trajković, V. S.. (2012). Graphene quantum dots as autophagy-inducing photodynamic agents. in Biomaterials, 33(29), 7084-7092.
https://doi.org/10.1016/j.biomaterials.2012.06.060

Marković ZM, Ristić BZ, Arsikin KM, Klisic DG, Harhaji-Trajković LM, Todorović-Marković B, Kepić DP, Kravić-Stevović TK, Jovanović SP, Milenković M, Milivojević D, Bumbaširević VŽ, Dramićanin M, Trajković VS. Graphene quantum dots as autophagy-inducing photodynamic agents. in Biomaterials. 2012;33(29):7084-7092.
doi:10.1016/j.biomaterials.2012.06.060 .

Marković, Zoran M., Ristić, Biljana Z., Arsikin, Katarina M., Klisic, Djordje G., Harhaji-Trajković, Ljubica M., Todorović-Marković, Biljana, Kepić, Dejan P., Kravić-Stevović, Tamara K., Jovanović, Svetlana P., Milenković, Marina, Milivojević, Dušan, Bumbaširević, Vladimir Ž., Dramićanin, Miroslav, Trajković, Vladimir S., "Graphene quantum dots as autophagy-inducing photodynamic agents" in Biomaterials, 33, no. 29 (2012):7084-7092,
https://doi.org/10.1016/j.biomaterials.2012.06.060 . .

TY  - JOUR
AU  - Vucicevic, Ljubica
AU  - Misirkić, Maja
AU  - Janjetović, Kristina D.
AU  - Vilimanovich, Urosh
AU  - Sudar, Emina
AU  - Isenović, Esma R.
AU  - Prica, Marko
AU  - Harhaji-Trajković, Ljubica M.
AU  - Kravić-Stevović, Tamara K.
AU  - Bumbaširević, Vladimir Ž.
AU  - Trajković, Vladimir S.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4180
AB  - In the present study, we report that compound C, an inhibitor of a key intracellular energy sensor AMP-activated protein kinase (AMPK), can induce autophagy in cancer cells. The induction of autophagy in U251 human glioma cell line was demonstrated by acridine orange staining of intracellular acidic vesicles, Beclin 1 induction, p62 decrease and conversion of LC3-I to autophagosome-associated LC3-II in the presence of proteolysis inhibitors. The presence of autophagosome-like vesicles was confirmed by transmission electron microscopy. Compound C-mediated inhibition of AMPK and raptor in U251 cells was associated with paradoxical decrease in phosphorylation of AMPK/raptor-repressed mTOR, a major negative regulator of autophagy, and its downstream target p70S6K. The phosphorylation of an mTOR activator Akt and the PI3K-activating kinase Src was also impaired in compound C-treated cells. The siRNA-mediated AMPK silencing did not reduce the activity of the Akt/mTOR/p70S6K pathway and AMPK activators metformin and AICAR failed to block compound C-induced autophagy. Autophagy inhibitors bafilomycin and chloroquine significantly increased the cytotoxicity of compound C towards U251 cells, as confirmed by increase in lactate dehydrogenase release, DNA fragmentation and caspase-3 activation. Similar effects of compound C were also observed in C6 rat glioma, L929 mouse fibrosarcoma and B16 mouse melanoma cell lines. Since compound C has previously been reported to suppress AMPK-dependent autophagy in different cell types, our findings suggest that the effects of compound C on autophagy might be dose-, cell type- and/or context-dependent. By demonstrating the ability of compound C to induce autophagic response in cancer cells via AMPK inhibition-independent downregulation of Akt/mTOR pathway, our results warrant caution when using compound C to inhibit AMPK-dependent cellular responses, but also support further exploration of compound C and related molecules as potential anticancer agents.
T2  - Autophagy
T1  - Compound C induces protective autophagy in cancer cells through AMPK inhibition-independent blockade of Akt/mTOR pathway
VL  - 7
IS  - 1
SP  - 40
EP  - 50
DO  - 10.4161/auto.7.1.13883
ER  - 

@article{
author = "Vucicevic, Ljubica and Misirkić, Maja and Janjetović, Kristina D. and Vilimanovich, Urosh and Sudar, Emina and Isenović, Esma R. and Prica, Marko and Harhaji-Trajković, Ljubica M. and Kravić-Stevović, Tamara K. and Bumbaširević, Vladimir Ž. and Trajković, Vladimir S.",
year = "2011",
abstract = "In the present study, we report that compound C, an inhibitor of a key intracellular energy sensor AMP-activated protein kinase (AMPK), can induce autophagy in cancer cells. The induction of autophagy in U251 human glioma cell line was demonstrated by acridine orange staining of intracellular acidic vesicles, Beclin 1 induction, p62 decrease and conversion of LC3-I to autophagosome-associated LC3-II in the presence of proteolysis inhibitors. The presence of autophagosome-like vesicles was confirmed by transmission electron microscopy. Compound C-mediated inhibition of AMPK and raptor in U251 cells was associated with paradoxical decrease in phosphorylation of AMPK/raptor-repressed mTOR, a major negative regulator of autophagy, and its downstream target p70S6K. The phosphorylation of an mTOR activator Akt and the PI3K-activating kinase Src was also impaired in compound C-treated cells. The siRNA-mediated AMPK silencing did not reduce the activity of the Akt/mTOR/p70S6K pathway and AMPK activators metformin and AICAR failed to block compound C-induced autophagy. Autophagy inhibitors bafilomycin and chloroquine significantly increased the cytotoxicity of compound C towards U251 cells, as confirmed by increase in lactate dehydrogenase release, DNA fragmentation and caspase-3 activation. Similar effects of compound C were also observed in C6 rat glioma, L929 mouse fibrosarcoma and B16 mouse melanoma cell lines. Since compound C has previously been reported to suppress AMPK-dependent autophagy in different cell types, our findings suggest that the effects of compound C on autophagy might be dose-, cell type- and/or context-dependent. By demonstrating the ability of compound C to induce autophagic response in cancer cells via AMPK inhibition-independent downregulation of Akt/mTOR pathway, our results warrant caution when using compound C to inhibit AMPK-dependent cellular responses, but also support further exploration of compound C and related molecules as potential anticancer agents.",
journal = "Autophagy",
title = "Compound C induces protective autophagy in cancer cells through AMPK inhibition-independent blockade of Akt/mTOR pathway",
volume = "7",
number = "1",
pages = "40-50",
doi = "10.4161/auto.7.1.13883"
}

Vucicevic, L., Misirkić, M., Janjetović, K. D., Vilimanovich, U., Sudar, E., Isenović, E. R., Prica, M., Harhaji-Trajković, L. M., Kravić-Stevović, T. K., Bumbaširević, V. Ž.,& Trajković, V. S.. (2011). Compound C induces protective autophagy in cancer cells through AMPK inhibition-independent blockade of Akt/mTOR pathway. in Autophagy, 7(1), 40-50.
https://doi.org/10.4161/auto.7.1.13883

Vucicevic L, Misirkić M, Janjetović KD, Vilimanovich U, Sudar E, Isenović ER, Prica M, Harhaji-Trajković LM, Kravić-Stevović TK, Bumbaširević VŽ, Trajković VS. Compound C induces protective autophagy in cancer cells through AMPK inhibition-independent blockade of Akt/mTOR pathway. in Autophagy. 2011;7(1):40-50.
doi:10.4161/auto.7.1.13883 .

Vucicevic, Ljubica, Misirkić, Maja, Janjetović, Kristina D., Vilimanovich, Urosh, Sudar, Emina, Isenović, Esma R., Prica, Marko, Harhaji-Trajković, Ljubica M., Kravić-Stevović, Tamara K., Bumbaširević, Vladimir Ž., Trajković, Vladimir S., "Compound C induces protective autophagy in cancer cells through AMPK inhibition-independent blockade of Akt/mTOR pathway" in Autophagy, 7, no. 1 (2011):40-50,
https://doi.org/10.4161/auto.7.1.13883 . .