Cirrone, Giuseppe Antonio Pablo

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  • Cirrone, Giuseppe Antonio Pablo (25)

Author's Bibliography

Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions

Keta, Otilija D.; Todorović, Danijela V.; Bulat, Tanja M.; Cirrone, Giuseppe Antonio Pablo; Romano, Francesco; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2017)

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Bulat, Tanja M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1573
AB  - The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied.
T2  - Experimental Biology and Medicine
T1  - Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions
VL  - 242
IS  - 10
SP  - 1015
EP  - 1024
DO  - 10.1177/1535370216669611
ER  - 
@article{
author = "Keta, Otilija D. and Todorović, Danijela V. and Bulat, Tanja M. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2017",
abstract = "The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied.",
journal = "Experimental Biology and Medicine",
title = "Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions",
volume = "242",
number = "10",
pages = "1015-1024",
doi = "10.1177/1535370216669611"
}
Keta, O. D., Todorović, D. V., Bulat, T. M., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2017). Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. in Experimental Biology and Medicine, 242(10), 1015-1024.
https://doi.org/10.1177/1535370216669611
Keta OD, Todorović DV, Bulat TM, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. in Experimental Biology and Medicine. 2017;242(10):1015-1024.
doi:10.1177/1535370216669611 .
Keta, Otilija D., Todorović, Danijela V., Bulat, Tanja M., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions" in Experimental Biology and Medicine, 242, no. 10 (2017):1015-1024,
https://doi.org/10.1177/1535370216669611 . .
10
10
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Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells

Žakula, Jelena; Korićanac, Lela; Keta, Otilija D.; Todorović, Danijela V.; Cirrone, Giuseppe Antonio Pablo; Romano, Francesco; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2016)

TY  - JOUR
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1290
AB  - Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.
T2  - Indian Journal of Medical Research
T1  - Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells
VL  - 143
SP  - 120
EP  - 128
DO  - 10.4103/0971-5916.191811
ER  - 
@article{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Todorović, Danijela V. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.",
journal = "Indian Journal of Medical Research",
title = "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells",
volume = "143",
pages = "120-128",
doi = "10.4103/0971-5916.191811"
}
Žakula, J., Korićanac, L., Keta, O. D., Todorović, D. V., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2016). Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research, 143, 120-128.
https://doi.org/10.4103/0971-5916.191811
Žakula J, Korićanac L, Keta OD, Todorović DV, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research. 2016;143:120-128.
doi:10.4103/0971-5916.191811 .
Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Todorović, Danijela V., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells" in Indian Journal of Medical Research, 143 (2016):120-128,
https://doi.org/10.4103/0971-5916.191811 . .
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Recent developments in Geant4

Allison, J; Amako, K; Apostolakis, J; Arce, P; Asai, M; Aso, T; Bagli, E; Bagulya, A; Banerjee, S; Barrand, G; Beck, B.R.; Bogdanov, A.G.; Brandt, D; Brown, J.M.C.; Burkhardt, H; Canal, Ph.; Cano-Ott, D; Chauvie, S; Cho, K; Cirrone, Giuseppe Antonio Pablo; Cooperman, G; Cortés-Giraldo, M.A.; Cosmo, G; Cuttone, Giacomo; Depaola, G; Desorgher, L; Dong, X; Dotti, A; Elvira, V.D.; Folger, G; Francis, Z; Galoyan, A; Garnier, L; Gayer, M; Genser, K.L.; Grichine, V.M.; Guatelli, S; Guèye, P; Gumplinger, P; Howard, A.S.; Hřivnáčová, I; Hwang, S; Incerti, S; Ivanchenko, A.; Ivanchenko, V.N.; Jones, F.W.; Jun, S.Y.; Kaitaniemi, P; Karakatsanis, N; Karamitros, M; Kelsey, M; Kimura, A; Koi, T; Kurashige, H; Lechner, A; Lee, S.B.; Longo, F; Maire, M; Mancusi, D; Mantero, A; Mendoza, E; Morgan, B; Murakami, K; Nikitina, T; Pandola, L; Paprocki, P; Perl, J; Petrović, Ivan M.; Pia, M.G.; Pokorski, W; Quesada, J.M.; Raine, M; Reis, M.A.; Ribon, A; Ristić-Fira, Aleksandra; Romano, Francesco; Russo, G; Santin, G; Sasaki, T; Sawkey, D; Shin, J.I.; Strakovsky, I.I.; Taborda, A; Tanaka, S; Tomé, B; Toshito, T; Tran, H.N.; Truscott, P.R.; Urban, L; Uzhinsky, V; Verbeke, J.M.; Verderi, M; Wendt, B.L.; Wenzel, H; Wright, D.H.; Wright, D.M.; Yamashita, T; Yarba, J; Yoshida, H

(2016)

TY  - JOUR
AU  - Allison, J
AU  - Amako, K
AU  - Apostolakis, J
AU  - Arce, P
AU  - Asai, M
AU  - Aso, T
AU  - Bagli, E
AU  - Bagulya, A
AU  - Banerjee, S
AU  - Barrand, G
AU  - Beck, B.R.
AU  - Bogdanov, A.G.
AU  - Brandt, D
AU  - Brown, J.M.C.
AU  - Burkhardt, H
AU  - Canal, Ph.
AU  - Cano-Ott, D
AU  - Chauvie, S
AU  - Cho, K
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cooperman, G
AU  - Cortés-Giraldo, M.A.
AU  - Cosmo, G
AU  - Cuttone, Giacomo
AU  - Depaola, G
AU  - Desorgher, L
AU  - Dong, X
AU  - Dotti, A
AU  - Elvira, V.D.
AU  - Folger, G
AU  - Francis, Z
AU  - Galoyan, A
AU  - Garnier, L
AU  - Gayer, M
AU  - Genser, K.L.
AU  - Grichine, V.M.
AU  - Guatelli, S
AU  - Guèye, P
AU  - Gumplinger, P
AU  - Howard, A.S.
AU  - Hřivnáčová, I
AU  - Hwang, S
AU  - Incerti, S
AU  - Ivanchenko, A.
AU  - Ivanchenko, V.N.
AU  - Jones, F.W.
AU  - Jun, S.Y.
AU  - Kaitaniemi, P
AU  - Karakatsanis, N
AU  - Karamitros, M
AU  - Kelsey, M
AU  - Kimura, A
AU  - Koi, T
AU  - Kurashige, H
AU  - Lechner, A
AU  - Lee, S.B.
AU  - Longo, F
AU  - Maire, M
AU  - Mancusi, D
AU  - Mantero, A
AU  - Mendoza, E
AU  - Morgan, B
AU  - Murakami, K
AU  - Nikitina, T
AU  - Pandola, L
AU  - Paprocki, P
AU  - Perl, J
AU  - Petrović, Ivan M.
AU  - Pia, M.G.
AU  - Pokorski, W
AU  - Quesada, J.M.
AU  - Raine, M
AU  - Reis, M.A.
AU  - Ribon, A
AU  - Ristić-Fira, Aleksandra
AU  - Romano, Francesco
AU  - Russo, G
AU  - Santin, G
AU  - Sasaki, T
AU  - Sawkey, D
AU  - Shin, J.I.
AU  - Strakovsky, I.I.
AU  - Taborda, A
AU  - Tanaka, S
AU  - Tomé, B
AU  - Toshito, T
AU  - Tran, H.N.
AU  - Truscott, P.R.
AU  - Urban, L
AU  - Uzhinsky, V
AU  - Verbeke, J.M.
AU  - Verderi, M
AU  - Wendt, B.L.
AU  - Wenzel, H
AU  - Wright, D.H.
AU  - Wright, D.M.
AU  - Yamashita, T
AU  - Yarba, J
AU  - Yoshida, H
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8645
AB  - Geant4 is a software toolkit for the simulation of the passage of particles through matter. It is used by a large number of experiments and projects in a variety of application domains, including high energy physics, astrophysics and space science, medical physics and radiation protection. Over the past several years, major changes have been made to the toolkit in order to accommodate the needs of these user communities, and to efficiently exploit the growth of computing power made available by advances in technology. The adaptation of Geant4 to multithreading, advances in physics, detector modeling and visualization, extensions to the toolkit, including biasing and reverse Monte Carlo, and tools for physics and release validation are discussed here.
T2  - Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
T1  - Recent developments in Geant4
VL  - 835
SP  - 186
EP  - 225
DO  - 10.1016/j.nima.2016.06.125
ER  - 
@article{
author = "Allison, J and Amako, K and Apostolakis, J and Arce, P and Asai, M and Aso, T and Bagli, E and Bagulya, A and Banerjee, S and Barrand, G and Beck, B.R. and Bogdanov, A.G. and Brandt, D and Brown, J.M.C. and Burkhardt, H and Canal, Ph. and Cano-Ott, D and Chauvie, S and Cho, K and Cirrone, Giuseppe Antonio Pablo and Cooperman, G and Cortés-Giraldo, M.A. and Cosmo, G and Cuttone, Giacomo and Depaola, G and Desorgher, L and Dong, X and Dotti, A and Elvira, V.D. and Folger, G and Francis, Z and Galoyan, A and Garnier, L and Gayer, M and Genser, K.L. and Grichine, V.M. and Guatelli, S and Guèye, P and Gumplinger, P and Howard, A.S. and Hřivnáčová, I and Hwang, S and Incerti, S and Ivanchenko, A. and Ivanchenko, V.N. and Jones, F.W. and Jun, S.Y. and Kaitaniemi, P and Karakatsanis, N and Karamitros, M and Kelsey, M and Kimura, A and Koi, T and Kurashige, H and Lechner, A and Lee, S.B. and Longo, F and Maire, M and Mancusi, D and Mantero, A and Mendoza, E and Morgan, B and Murakami, K and Nikitina, T and Pandola, L and Paprocki, P and Perl, J and Petrović, Ivan M. and Pia, M.G. and Pokorski, W and Quesada, J.M. and Raine, M and Reis, M.A. and Ribon, A and Ristić-Fira, Aleksandra and Romano, Francesco and Russo, G and Santin, G and Sasaki, T and Sawkey, D and Shin, J.I. and Strakovsky, I.I. and Taborda, A and Tanaka, S and Tomé, B and Toshito, T and Tran, H.N. and Truscott, P.R. and Urban, L and Uzhinsky, V and Verbeke, J.M. and Verderi, M and Wendt, B.L. and Wenzel, H and Wright, D.H. and Wright, D.M. and Yamashita, T and Yarba, J and Yoshida, H",
year = "2016",
abstract = "Geant4 is a software toolkit for the simulation of the passage of particles through matter. It is used by a large number of experiments and projects in a variety of application domains, including high energy physics, astrophysics and space science, medical physics and radiation protection. Over the past several years, major changes have been made to the toolkit in order to accommodate the needs of these user communities, and to efficiently exploit the growth of computing power made available by advances in technology. The adaptation of Geant4 to multithreading, advances in physics, detector modeling and visualization, extensions to the toolkit, including biasing and reverse Monte Carlo, and tools for physics and release validation are discussed here.",
journal = "Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment",
title = "Recent developments in Geant4",
volume = "835",
pages = "186-225",
doi = "10.1016/j.nima.2016.06.125"
}
Allison, J., Amako, K., Apostolakis, J., Arce, P., Asai, M., Aso, T., Bagli, E., Bagulya, A., Banerjee, S., Barrand, G., Beck, B.R., Bogdanov, A.G., Brandt, D., Brown, J.M.C., Burkhardt, H., Canal, Ph., Cano-Ott, D., Chauvie, S., Cho, K., Cirrone, G. A. P., Cooperman, G., Cortés-Giraldo, M.A., Cosmo, G., Cuttone, G., Depaola, G., Desorgher, L., Dong, X., Dotti, A., Elvira, V.D., Folger, G., Francis, Z., Galoyan, A., Garnier, L., Gayer, M., Genser, K.L., Grichine, V.M., Guatelli, S., Guèye, P., Gumplinger, P., Howard, A.S., Hřivnáčová, I., Hwang, S., Incerti, S., Ivanchenko, A., Ivanchenko, V.N., Jones, F.W., Jun, S.Y., Kaitaniemi, P., Karakatsanis, N., Karamitros, M., Kelsey, M., Kimura, A., Koi, T., Kurashige, H., Lechner, A., Lee, S.B., Longo, F., Maire, M., Mancusi, D., Mantero, A., Mendoza, E., Morgan, B., Murakami, K., Nikitina, T., Pandola, L., Paprocki, P., Perl, J., Petrović, I. M., Pia, M.G., Pokorski, W., Quesada, J.M., Raine, M., Reis, M.A., Ribon, A., Ristić-Fira, A., Romano, F., Russo, G., Santin, G., Sasaki, T., Sawkey, D., Shin, J.I., Strakovsky, I.I., Taborda, A., Tanaka, S., Tomé, B., Toshito, T., Tran, H.N., Truscott, P.R., Urban, L., Uzhinsky, V., Verbeke, J.M., Verderi, M., Wendt, B.L., Wenzel, H., Wright, D.H., Wright, D.M., Yamashita, T., Yarba, J.,& Yoshida, H.. (2016). Recent developments in Geant4. in Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 835, 186-225.
https://doi.org/10.1016/j.nima.2016.06.125
Allison J, Amako K, Apostolakis J, Arce P, Asai M, Aso T, Bagli E, Bagulya A, Banerjee S, Barrand G, Beck B, Bogdanov A, Brandt D, Brown J, Burkhardt H, Canal P, Cano-Ott D, Chauvie S, Cho K, Cirrone GAP, Cooperman G, Cortés-Giraldo M, Cosmo G, Cuttone G, Depaola G, Desorgher L, Dong X, Dotti A, Elvira V, Folger G, Francis Z, Galoyan A, Garnier L, Gayer M, Genser K, Grichine V, Guatelli S, Guèye P, Gumplinger P, Howard A, Hřivnáčová I, Hwang S, Incerti S, Ivanchenko A, Ivanchenko V, Jones F, Jun S, Kaitaniemi P, Karakatsanis N, Karamitros M, Kelsey M, Kimura A, Koi T, Kurashige H, Lechner A, Lee S, Longo F, Maire M, Mancusi D, Mantero A, Mendoza E, Morgan B, Murakami K, Nikitina T, Pandola L, Paprocki P, Perl J, Petrović IM, Pia M, Pokorski W, Quesada J, Raine M, Reis M, Ribon A, Ristić-Fira A, Romano F, Russo G, Santin G, Sasaki T, Sawkey D, Shin J, Strakovsky I, Taborda A, Tanaka S, Tomé B, Toshito T, Tran H, Truscott P, Urban L, Uzhinsky V, Verbeke J, Verderi M, Wendt B, Wenzel H, Wright D, Wright D, Yamashita T, Yarba J, Yoshida H. Recent developments in Geant4. in Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. 2016;835:186-225.
doi:10.1016/j.nima.2016.06.125 .
Allison, J, Amako, K, Apostolakis, J, Arce, P, Asai, M, Aso, T, Bagli, E, Bagulya, A, Banerjee, S, Barrand, G, Beck, B.R., Bogdanov, A.G., Brandt, D, Brown, J.M.C., Burkhardt, H, Canal, Ph., Cano-Ott, D, Chauvie, S, Cho, K, Cirrone, Giuseppe Antonio Pablo, Cooperman, G, Cortés-Giraldo, M.A., Cosmo, G, Cuttone, Giacomo, Depaola, G, Desorgher, L, Dong, X, Dotti, A, Elvira, V.D., Folger, G, Francis, Z, Galoyan, A, Garnier, L, Gayer, M, Genser, K.L., Grichine, V.M., Guatelli, S, Guèye, P, Gumplinger, P, Howard, A.S., Hřivnáčová, I, Hwang, S, Incerti, S, Ivanchenko, A., Ivanchenko, V.N., Jones, F.W., Jun, S.Y., Kaitaniemi, P, Karakatsanis, N, Karamitros, M, Kelsey, M, Kimura, A, Koi, T, Kurashige, H, Lechner, A, Lee, S.B., Longo, F, Maire, M, Mancusi, D, Mantero, A, Mendoza, E, Morgan, B, Murakami, K, Nikitina, T, Pandola, L, Paprocki, P, Perl, J, Petrović, Ivan M., Pia, M.G., Pokorski, W, Quesada, J.M., Raine, M, Reis, M.A., Ribon, A, Ristić-Fira, Aleksandra, Romano, Francesco, Russo, G, Santin, G, Sasaki, T, Sawkey, D, Shin, J.I., Strakovsky, I.I., Taborda, A, Tanaka, S, Tomé, B, Toshito, T, Tran, H.N., Truscott, P.R., Urban, L, Uzhinsky, V, Verbeke, J.M., Verderi, M, Wendt, B.L., Wenzel, H, Wright, D.H., Wright, D.M., Yamashita, T, Yarba, J, Yoshida, H, "Recent developments in Geant4" in Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 835 (2016):186-225,
https://doi.org/10.1016/j.nima.2016.06.125 . .
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ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines

Schillaci, F.; Anzalone, A.; Cirrone, Giuseppe Antonio Pablo; Carpinelli, M.; Cuttone, Giacomo; Cutroneo, M.; De Martinis, C.; Giove, D.; Korn, G.; Maggiore, M.; Manti, L.; Margarone, D.; Musumarra, A.; Perozziello, F. M.; Petrović, Ivan M.; Pisciotta, P.; Renis, M.; Ristić-Fira, Aleksandra; Romano, Francesco; Romano, Francesco; Schettino, G.; Scuderi, V.; Torrisi, L.; Tramontana, A.; Tudisco, S.

(2014)

TY  - CONF
AU  - Schillaci, F.
AU  - Anzalone, A.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Carpinelli, M.
AU  - Cuttone, Giacomo
AU  - Cutroneo, M.
AU  - De Martinis, C.
AU  - Giove, D.
AU  - Korn, G.
AU  - Maggiore, M.
AU  - Manti, L.
AU  - Margarone, D.
AU  - Musumarra, A.
AU  - Perozziello, F. M.
AU  - Petrović, Ivan M.
AU  - Pisciotta, P.
AU  - Renis, M.
AU  - Ristić-Fira, Aleksandra
AU  - Romano, Francesco
AU  - Romano, Francesco
AU  - Schettino, G.
AU  - Scuderi, V.
AU  - Torrisi, L.
AU  - Tramontana, A.
AU  - Tudisco, S.
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7029
AB  - ELI-Beamlines is one of the pillars of the pan-European project ELI (Extreme Light Infrastructure). It will be an ultra high-intensity, high repetition-rate, femtosecond laser facility whose main goal is generation and applications of high-brightness X-ray sources and accelerated charged particles in different fields. Particular care will be devoted to the potential applicability of laser-driven ion beams for medical treatments of tumors. Indeed, such kind of beams show very interesting peculiarities and, moreover, laser-driven based accelerators can really represent a competitive alternative to conventional machines since they are expected to be more compact in size and less expensive. The ELIMED project was launched thanks to a collaboration established between FZU-ASCR (ELI-Beamlines) and INFN-LNS researchers. Several European institutes have already shown a great interest in the project aiming to explore the possibility to use laser-driven ion (mostly proton) beams for several applications with a particular regard for medical ones. To reach the project goal several tasks need to be fulfilled, starting from the optimization of laser-target interaction to dosimetric studies at the irradiation point at the end of a proper designed transport beam-line. Researchers from LNS have already developed and successfully tested a high-dispersive power Thomson Parabola Spectrometer, which is the first prototype of a more performing device to be used within the ELIMED project. Also a Magnetic Selection System able to produce a small pencil beam out of a wide energy distribution of ions produced in laser-target interaction has been realized and some preliminary work for its testing and characterization is in progress. In this contribution the status of the project will be reported together with a short description of the of the features of device recently developed.
C3  - Journal of Physics: Conference Series
T1  - ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines
VL  - 508
DO  - 10.1088/1742-6596/508/1/012010
ER  - 
@conference{
author = "Schillaci, F. and Anzalone, A. and Cirrone, Giuseppe Antonio Pablo and Carpinelli, M. and Cuttone, Giacomo and Cutroneo, M. and De Martinis, C. and Giove, D. and Korn, G. and Maggiore, M. and Manti, L. and Margarone, D. and Musumarra, A. and Perozziello, F. M. and Petrović, Ivan M. and Pisciotta, P. and Renis, M. and Ristić-Fira, Aleksandra and Romano, Francesco and Romano, Francesco and Schettino, G. and Scuderi, V. and Torrisi, L. and Tramontana, A. and Tudisco, S.",
year = "2014",
abstract = "ELI-Beamlines is one of the pillars of the pan-European project ELI (Extreme Light Infrastructure). It will be an ultra high-intensity, high repetition-rate, femtosecond laser facility whose main goal is generation and applications of high-brightness X-ray sources and accelerated charged particles in different fields. Particular care will be devoted to the potential applicability of laser-driven ion beams for medical treatments of tumors. Indeed, such kind of beams show very interesting peculiarities and, moreover, laser-driven based accelerators can really represent a competitive alternative to conventional machines since they are expected to be more compact in size and less expensive. The ELIMED project was launched thanks to a collaboration established between FZU-ASCR (ELI-Beamlines) and INFN-LNS researchers. Several European institutes have already shown a great interest in the project aiming to explore the possibility to use laser-driven ion (mostly proton) beams for several applications with a particular regard for medical ones. To reach the project goal several tasks need to be fulfilled, starting from the optimization of laser-target interaction to dosimetric studies at the irradiation point at the end of a proper designed transport beam-line. Researchers from LNS have already developed and successfully tested a high-dispersive power Thomson Parabola Spectrometer, which is the first prototype of a more performing device to be used within the ELIMED project. Also a Magnetic Selection System able to produce a small pencil beam out of a wide energy distribution of ions produced in laser-target interaction has been realized and some preliminary work for its testing and characterization is in progress. In this contribution the status of the project will be reported together with a short description of the of the features of device recently developed.",
journal = "Journal of Physics: Conference Series",
title = "ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines",
volume = "508",
doi = "10.1088/1742-6596/508/1/012010"
}
Schillaci, F., Anzalone, A., Cirrone, G. A. P., Carpinelli, M., Cuttone, G., Cutroneo, M., De Martinis, C., Giove, D., Korn, G., Maggiore, M., Manti, L., Margarone, D., Musumarra, A., Perozziello, F. M., Petrović, I. M., Pisciotta, P., Renis, M., Ristić-Fira, A., Romano, F., Romano, F., Schettino, G., Scuderi, V., Torrisi, L., Tramontana, A.,& Tudisco, S.. (2014). ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines. in Journal of Physics: Conference Series, 508.
https://doi.org/10.1088/1742-6596/508/1/012010
Schillaci F, Anzalone A, Cirrone GAP, Carpinelli M, Cuttone G, Cutroneo M, De Martinis C, Giove D, Korn G, Maggiore M, Manti L, Margarone D, Musumarra A, Perozziello FM, Petrović IM, Pisciotta P, Renis M, Ristić-Fira A, Romano F, Romano F, Schettino G, Scuderi V, Torrisi L, Tramontana A, Tudisco S. ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines. in Journal of Physics: Conference Series. 2014;508.
doi:10.1088/1742-6596/508/1/012010 .
Schillaci, F., Anzalone, A., Cirrone, Giuseppe Antonio Pablo, Carpinelli, M., Cuttone, Giacomo, Cutroneo, M., De Martinis, C., Giove, D., Korn, G., Maggiore, M., Manti, L., Margarone, D., Musumarra, A., Perozziello, F. M., Petrović, Ivan M., Pisciotta, P., Renis, M., Ristić-Fira, Aleksandra, Romano, Francesco, Romano, Francesco, Schettino, G., Scuderi, V., Torrisi, L., Tramontana, A., Tudisco, S., "ELIMED, MEDical and multidisciplinary applications at ELI-Beamlines" in Journal of Physics: Conference Series, 508 (2014),
https://doi.org/10.1088/1742-6596/508/1/012010 . .
1
17
15
17

A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line

Romano, Francesco; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Di Rosa, F.; Mazzaglia, S. E.; Petrović, Ivan M.; Ristić-Fira, Aleksandra; Varisano, A.

(2014)

TY  - JOUR
AU  - Romano, Francesco
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Di Rosa, F.
AU  - Mazzaglia, S. E.
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Varisano, A.
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6037
AB  - Fluence, depth absorbed dose and linear energy transfer (LET) distributions of proton and carbon ion beams have been investigated using the Monte Carlo code Geant4 (GEometry ANd Tracking). An open source application was developed with the aim to simulate two typical transport beam lines, one used for ocular therapy and cell irradiations with protons and the other for cell irradiations with carbon ions. This tool allows evaluation of the primary and total dose averaged LET and predict their spatial distribution in voxelized or sliced geometries. In order to reproduce the LET distributions in a realistic way, and also the secondary particles contributions due to nuclear interactions were considered in the computations. Pristine and spread-out Bragg peaks were taken into account both for proton and carbon ion beams, with the maximum energy of 62 MeV/n. Depth dose distributions were compared with experimental data, showing good agreement. Primary and total LET distributions were analysed in order to study the influence of contributions of secondary particles in regions at different depths. A non-negligible influence of high-LET components was found in the entrance channel for proton beams, determining the total dose averaged LET by the factor 3 higher than the primary one. A completely different situation was obtained for carbon ions. In this case, secondary particles mainly contributed in the tail that is after the peak. The results showed how the weight of light and heavy secondary ions can considerably influence the computation of LET depth distributions. This has an important role in the interpretation of results coming from radiobiological experiments and, therefore, in hadron treatment planning procedures.
T2  - Physics in Medicine and Biology
T1  - A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line
VL  - 59
IS  - 12
SP  - 2863
EP  - 2882
DO  - 10.1088/0031-9155/59/12/2863
ER  - 
@article{
author = "Romano, Francesco and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Di Rosa, F. and Mazzaglia, S. E. and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Varisano, A.",
year = "2014",
abstract = "Fluence, depth absorbed dose and linear energy transfer (LET) distributions of proton and carbon ion beams have been investigated using the Monte Carlo code Geant4 (GEometry ANd Tracking). An open source application was developed with the aim to simulate two typical transport beam lines, one used for ocular therapy and cell irradiations with protons and the other for cell irradiations with carbon ions. This tool allows evaluation of the primary and total dose averaged LET and predict their spatial distribution in voxelized or sliced geometries. In order to reproduce the LET distributions in a realistic way, and also the secondary particles contributions due to nuclear interactions were considered in the computations. Pristine and spread-out Bragg peaks were taken into account both for proton and carbon ion beams, with the maximum energy of 62 MeV/n. Depth dose distributions were compared with experimental data, showing good agreement. Primary and total LET distributions were analysed in order to study the influence of contributions of secondary particles in regions at different depths. A non-negligible influence of high-LET components was found in the entrance channel for proton beams, determining the total dose averaged LET by the factor 3 higher than the primary one. A completely different situation was obtained for carbon ions. In this case, secondary particles mainly contributed in the tail that is after the peak. The results showed how the weight of light and heavy secondary ions can considerably influence the computation of LET depth distributions. This has an important role in the interpretation of results coming from radiobiological experiments and, therefore, in hadron treatment planning procedures.",
journal = "Physics in Medicine and Biology",
title = "A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line",
volume = "59",
number = "12",
pages = "2863-2882",
doi = "10.1088/0031-9155/59/12/2863"
}
Romano, F., Cirrone, G. A. P., Cuttone, G., Di Rosa, F., Mazzaglia, S. E., Petrović, I. M., Ristić-Fira, A.,& Varisano, A.. (2014). A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line. in Physics in Medicine and Biology, 59(12), 2863-2882.
https://doi.org/10.1088/0031-9155/59/12/2863
Romano F, Cirrone GAP, Cuttone G, Di Rosa F, Mazzaglia SE, Petrović IM, Ristić-Fira A, Varisano A. A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line. in Physics in Medicine and Biology. 2014;59(12):2863-2882.
doi:10.1088/0031-9155/59/12/2863 .
Romano, Francesco, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Di Rosa, F., Mazzaglia, S. E., Petrović, Ivan M., Ristić-Fira, Aleksandra, Varisano, A., "A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line" in Physics in Medicine and Biology, 59, no. 12 (2014):2863-2882,
https://doi.org/10.1088/0031-9155/59/12/2863 . .
1
54
46
47

Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED

Ristić-Fira, Aleksandra; Bulat, Tanja M.; Keta, Otilija D.; Romano, Francesco; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Petrović, Ivan M.

(2013)

TY  - CONF
AU  - Ristić-Fira, Aleksandra
AU  - Bulat, Tanja M.
AU  - Keta, Otilija D.
AU  - Romano, Francesco
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7005
AB  - The aim of this study is to investigate the behavior of radio-resistant human malignant cells, thus enabling better understanding of radiobiological effects of ions in such a case. Radiation sources such as accelerated continuous ion beams and laser technology-based ultra short radiation sources with energy of around 10 MeV will be used. The HTB140 melanoma cells are chosen since it has been shown that they represent the limit case of cellular radio-resistance among the studied tumour cell lines. These cells are particularly interesting as they provide data on the very edge of inactivation capacity of each beam line that is tested. After exposing the cell monolayers to continuous radiations of low (gamma-rays) and high (protons) linear energy transfer, the kinetics of disappearance of the phosphorylated histone H2AX (gamma-H2AX) foci per cell will be determined. The same procedure will be performed with the pulsed high dose rate protons. Detection and quantification of gamma-H2AX foci will be performed by immunohistochemical 3D time-dependent imaging analyses using laser scanning confocal microscopy. Immunoblotting will enable the follow-up of the relation between gamma-H2AX and cell cycle arrest via the p53/p21 pathway. In such a way the spatio-temporal changes on sub-cellular level will be visualized, quantified and compared. These results will show whether there is a difference in the effects on cells between continuous and pulsed irradiation mode. Therefore, they will contribute to the database that might promote pulsed sources for medical treatments of malignant growths.
C3  - AIP Conference Proceedings
T1  - Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED
VL  - 1546
SP  - 101
EP  - 104
DO  - 10.1063/1.4816616
ER  - 
@conference{
author = "Ristić-Fira, Aleksandra and Bulat, Tanja M. and Keta, Otilija D. and Romano, Francesco and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2013",
abstract = "The aim of this study is to investigate the behavior of radio-resistant human malignant cells, thus enabling better understanding of radiobiological effects of ions in such a case. Radiation sources such as accelerated continuous ion beams and laser technology-based ultra short radiation sources with energy of around 10 MeV will be used. The HTB140 melanoma cells are chosen since it has been shown that they represent the limit case of cellular radio-resistance among the studied tumour cell lines. These cells are particularly interesting as they provide data on the very edge of inactivation capacity of each beam line that is tested. After exposing the cell monolayers to continuous radiations of low (gamma-rays) and high (protons) linear energy transfer, the kinetics of disappearance of the phosphorylated histone H2AX (gamma-H2AX) foci per cell will be determined. The same procedure will be performed with the pulsed high dose rate protons. Detection and quantification of gamma-H2AX foci will be performed by immunohistochemical 3D time-dependent imaging analyses using laser scanning confocal microscopy. Immunoblotting will enable the follow-up of the relation between gamma-H2AX and cell cycle arrest via the p53/p21 pathway. In such a way the spatio-temporal changes on sub-cellular level will be visualized, quantified and compared. These results will show whether there is a difference in the effects on cells between continuous and pulsed irradiation mode. Therefore, they will contribute to the database that might promote pulsed sources for medical treatments of malignant growths.",
journal = "AIP Conference Proceedings",
title = "Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED",
volume = "1546",
pages = "101-104",
doi = "10.1063/1.4816616"
}
Ristić-Fira, A., Bulat, T. M., Keta, O. D., Romano, F., Cirrone, G. A. P., Cuttone, G.,& Petrović, I. M.. (2013). Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED. in AIP Conference Proceedings, 1546, 101-104.
https://doi.org/10.1063/1.4816616
Ristić-Fira A, Bulat TM, Keta OD, Romano F, Cirrone GAP, Cuttone G, Petrović IM. Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED. in AIP Conference Proceedings. 2013;1546:101-104.
doi:10.1063/1.4816616 .
Ristić-Fira, Aleksandra, Bulat, Tanja M., Keta, Otilija D., Romano, Francesco, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Petrović, Ivan M., "Spatio-Temporal Radiation Biology with Conventionally or Laser-Accelerated Particles for ELIMED" in AIP Conference Proceedings, 1546 (2013):101-104,
https://doi.org/10.1063/1.4816616 . .

Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells

Korićanac, Lela; Žakula, Jelena; Keta, Otilija D.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra; Petrović, Ivan M.

(2013)

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2013
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5593
AB  - This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation.
T2  - Nuclear technology and radiation protection
T1  - Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells
VL  - 28
IS  - 2
SP  - 195
EP  - 203
DO  - 10.2298/NTRP1302195K
ER  - 
@article{
author = "Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2013",
abstract = "This study was conducted in order to evaluate the ability of carbon ions to induce DNA double-strand breaks and apoptosis in the radio-resistant human HTB140 melanoma cells. The cells were irradiated with C-12 ions having the linear energy transfer of 258 keV/mu m. Irradiations were performed in the dose range from 2 to 16 Gy. Induction of DNA double-strand breaks was evaluated 2 hour after irradiation through expression of gamma H2AX protein. Increased level of gamma H2AX detected in irradiated samples was especially high after irradiation with 12 and 16 Gy. Dose dependent increase of apoptosis was detected 48 hour after irradiation by flow-cytometry, with the maximum value of 20.4% after irradiation with 16 Gy, and the apoptotic index of 9.3. Pro-apoptotic effects of carbon ion beams were confirmed by changes of key molecules of the mitochondrial apoptotic pathway, p53 protein expression, Bax/Bcl-2 ratio and caspase-3 activation.",
journal = "Nuclear technology and radiation protection",
title = "Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells",
volume = "28",
number = "2",
pages = "195-203",
doi = "10.2298/NTRP1302195K"
}
Korićanac, L., Žakula, J., Keta, O. D., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2013). Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells. in Nuclear technology and radiation protection, 28(2), 195-203.
https://doi.org/10.2298/NTRP1302195K
Korićanac L, Žakula J, Keta OD, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells. in Nuclear technology and radiation protection. 2013;28(2):195-203.
doi:10.2298/NTRP1302195K .
Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Carbon Ions Induce DNA Double Strand Breaks and Apoptosis in Htb140 Melanoma Cells" in Nuclear technology and radiation protection, 28, no. 2 (2013):195-203,
https://doi.org/10.2298/NTRP1302195K . .
2
2
2

Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET

Petrović, Ivan M.; Ristić-Fira, Aleksandra; Todorović, Dragana; Korićanac, Lela; Žakula, Jelena; Cirrone, Giuseppe Antonio Pablo; Romano, Francesco; Cuttone, Giacomo

(2012)

TY  - CONF
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Dragana
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8643
C3  - Radiotherapy and Oncology
T1  - Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET
VL  - 102
SP  - S108
EP  - S109
DO  - 10.1016/S0167-8140(12)70185-8
ER  - 
@conference{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Dragana and Korićanac, Lela and Žakula, Jelena and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo",
year = "2012",
journal = "Radiotherapy and Oncology",
title = "Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET",
volume = "102",
pages = "S108-S109",
doi = "10.1016/S0167-8140(12)70185-8"
}
Petrović, I. M., Ristić-Fira, A., Todorović, D., Korićanac, L., Žakula, J., Cirrone, G. A. P., Romano, F.,& Cuttone, G.. (2012). Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET. in Radiotherapy and Oncology, 102, S108-S109.
https://doi.org/10.1016/S0167-8140(12)70185-8
Petrović IM, Ristić-Fira A, Todorović D, Korićanac L, Žakula J, Cirrone GAP, Romano F, Cuttone G. Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET. in Radiotherapy and Oncology. 2012;102:S108-S109.
doi:10.1016/S0167-8140(12)70185-8 .
Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Dragana, Korićanac, Lela, Žakula, Jelena, Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, "Radio-resistant human malignant cells after irradiations with 1H and 12C ions of different LET" in Radiotherapy and Oncology, 102 (2012):S108-S109,
https://doi.org/10.1016/S0167-8140(12)70185-8 . .
1

Response of human lung adenocarcinoma cells to proton radiation and erlotinib

Ristić-Fira, Aleksandra; Petrović, Ivan M.; Todorović, Dragana; Korićanac, Lela; Keta, Otilija D.; Bulat, Tanja M.; Cirrone, Giuseppe Antonio Pablo; Romano, Francesco; Cuttone, Giacomo

(2012)

TY  - CONF
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Todorović, Dragana
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8642
C3  - Radiotherapy and Oncology
T1  - Response of human lung adenocarcinoma cells to proton radiation and erlotinib
VL  - 102
SP  - S106
EP  - S107
DO  - 10.1016/S0167-8140(12)70182-2
ER  - 
@conference{
author = "Ristić-Fira, Aleksandra and Petrović, Ivan M. and Todorović, Dragana and Korićanac, Lela and Keta, Otilija D. and Bulat, Tanja M. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo",
year = "2012",
journal = "Radiotherapy and Oncology",
title = "Response of human lung adenocarcinoma cells to proton radiation and erlotinib",
volume = "102",
pages = "S106-S107",
doi = "10.1016/S0167-8140(12)70182-2"
}
Ristić-Fira, A., Petrović, I. M., Todorović, D., Korićanac, L., Keta, O. D., Bulat, T. M., Cirrone, G. A. P., Romano, F.,& Cuttone, G.. (2012). Response of human lung adenocarcinoma cells to proton radiation and erlotinib. in Radiotherapy and Oncology, 102, S106-S107.
https://doi.org/10.1016/S0167-8140(12)70182-2
Ristić-Fira A, Petrović IM, Todorović D, Korićanac L, Keta OD, Bulat TM, Cirrone GAP, Romano F, Cuttone G. Response of human lung adenocarcinoma cells to proton radiation and erlotinib. in Radiotherapy and Oncology. 2012;102:S106-S107.
doi:10.1016/S0167-8140(12)70182-2 .
Ristić-Fira, Aleksandra, Petrović, Ivan M., Todorović, Dragana, Korićanac, Lela, Keta, Otilija D., Bulat, Tanja M., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, "Response of human lung adenocarcinoma cells to proton radiation and erlotinib" in Radiotherapy and Oncology, 102 (2012):S106-S107,
https://doi.org/10.1016/S0167-8140(12)70182-2 . .

Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line

Korićanac, Lela; Žakula, Jelena; Cirrone, Giuseppe Antonio Pablo; Privitera, Giuseppe; Cuttone, Giacomo; Petrović, Ivan M.; Ristić-Fira, Aleksandra

(2012)

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8644
AB  - The effects of combined treatments with fotemustine and proton radiation on cell proliferation and induction of apoptosis have been analyzed in this study. HTB140 human melanoma cells were treated with fotemustine (100, 250 M) 24 h prior to irradiation (12, 16 Gy). The cells were irradiated in the middle of a therapeutic 62 MeV proton spread-out Bragg peak. An efficiency of applied treatments was observed throughout the evaluation of the cell proliferation 7 days after proton irradiation. The combined treatments with fotemustine and protons resulted in a greater antiproliferative response than each treatment alone. The number of apoptotic cells was estimated after 6 or 48 h using flow cytometry. The highest percentage of apoptotic cells was obtained 48 h after treatment with 250 M fotemustine and protons. Western blot analysis showed that induction of apoptosis was associated with p53 and Bax up regulation, and Bcl-2 down regulation. The induction of a caspase-3 activity and cleavage of PARP were clearly observed. These data indicate that a combined application of FM and proton irradiation is more effective in reducing melanoma cell proliferation and the induction of apoptosis, suggesting that FM can increase the radio-sensitivity of HTB140 melanoma cells.
T2  - Advanced Science Letters
T1  - Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line
VL  - 5
IS  - 2
SP  - 552
EP  - 559
DO  - 10.1166/asl.2012.2150
ER  - 
@article{
author = "Korićanac, Lela and Žakula, Jelena and Cirrone, Giuseppe Antonio Pablo and Privitera, Giuseppe and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2012",
abstract = "The effects of combined treatments with fotemustine and proton radiation on cell proliferation and induction of apoptosis have been analyzed in this study. HTB140 human melanoma cells were treated with fotemustine (100, 250 M) 24 h prior to irradiation (12, 16 Gy). The cells were irradiated in the middle of a therapeutic 62 MeV proton spread-out Bragg peak. An efficiency of applied treatments was observed throughout the evaluation of the cell proliferation 7 days after proton irradiation. The combined treatments with fotemustine and protons resulted in a greater antiproliferative response than each treatment alone. The number of apoptotic cells was estimated after 6 or 48 h using flow cytometry. The highest percentage of apoptotic cells was obtained 48 h after treatment with 250 M fotemustine and protons. Western blot analysis showed that induction of apoptosis was associated with p53 and Bax up regulation, and Bcl-2 down regulation. The induction of a caspase-3 activity and cleavage of PARP were clearly observed. These data indicate that a combined application of FM and proton irradiation is more effective in reducing melanoma cell proliferation and the induction of apoptosis, suggesting that FM can increase the radio-sensitivity of HTB140 melanoma cells.",
journal = "Advanced Science Letters",
title = "Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line",
volume = "5",
number = "2",
pages = "552-559",
doi = "10.1166/asl.2012.2150"
}
Korićanac, L., Žakula, J., Cirrone, G. A. P., Privitera, G., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2012). Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line. in Advanced Science Letters, 5(2), 552-559.
https://doi.org/10.1166/asl.2012.2150
Korićanac L, Žakula J, Cirrone GAP, Privitera G, Cuttone G, Petrović IM, Ristić-Fira A. Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line. in Advanced Science Letters. 2012;5(2):552-559.
doi:10.1166/asl.2012.2150 .
Korićanac, Lela, Žakula, Jelena, Cirrone, Giuseppe Antonio Pablo, Privitera, Giuseppe, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Variation of Apoptotic Pathway Regulators by Fotemustine and Protons in a Human Melanoma Cell Line" in Advanced Science Letters, 5, no. 2 (2012):552-559,
https://doi.org/10.1166/asl.2012.2150 . .
1
1

Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons

Ristić-Fira, Aleksandra; Todorović, Danijela V.; Žakula, Jelena; Keta, Otilija D.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Petrović, Ivan M.

(2011)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4537
AB  - Conventional radiotherapy with X-and gamma-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and (12)C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the radio-resistant human HTB140 melanoma cells. The cells were irradiated with gamma-rays, the 62 MeV protons at the Bragg peak and in the middle of the spread-out Bragg peak (SOBP), as well as with the 62 MeV/u (12)C ions. The doses ranged from 2 to 24 Gy. Cell survival and proliferation were assessed 7 days after irradiation, whereas apoptosis was evaluated after 48 h. The acquired results confirmed the high radio-resistance of cells, showing better effectiveness of protons than gamma-rays. The best efficiency was obtained with (12)C ions due to higher linear energy transfer. All analyzed radiation qualities reduced cell proliferation. The highest proliferation was detected for (12)C ions because of their large killing capacity followed by small induction of reparable lesions. This enabled unharmed cells to preserve proliferative activity. Irradiations with protons and (12)C ions revealed similar moderate pro-apoptotic ability that is in agreement with the level of cellular radio-resistance.
T2  - Physiological Research
T1  - Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons
VL  - 60
SP  - S129
EP  - S135
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Žakula, Jelena and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2011",
abstract = "Conventional radiotherapy with X-and gamma-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and (12)C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the radio-resistant human HTB140 melanoma cells. The cells were irradiated with gamma-rays, the 62 MeV protons at the Bragg peak and in the middle of the spread-out Bragg peak (SOBP), as well as with the 62 MeV/u (12)C ions. The doses ranged from 2 to 24 Gy. Cell survival and proliferation were assessed 7 days after irradiation, whereas apoptosis was evaluated after 48 h. The acquired results confirmed the high radio-resistance of cells, showing better effectiveness of protons than gamma-rays. The best efficiency was obtained with (12)C ions due to higher linear energy transfer. All analyzed radiation qualities reduced cell proliferation. The highest proliferation was detected for (12)C ions because of their large killing capacity followed by small induction of reparable lesions. This enabled unharmed cells to preserve proliferative activity. Irradiations with protons and (12)C ions revealed similar moderate pro-apoptotic ability that is in agreement with the level of cellular radio-resistance.",
journal = "Physiological Research",
title = "Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons",
volume = "60",
pages = "S129-S135"
}
Ristić-Fira, A., Todorović, D. V., Žakula, J., Keta, O. D., Cirrone, G. A. P., Cuttone, G.,& Petrović, I. M.. (2011). Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons. in Physiological Research, 60, S129-S135.
Ristić-Fira A, Todorović DV, Žakula J, Keta OD, Cirrone GAP, Cuttone G, Petrović IM. Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons. in Physiological Research. 2011;60:S129-S135..
Ristić-Fira, Aleksandra, Todorović, Danijela V., Žakula, Jelena, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Petrović, Ivan M., "Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons" in Physiological Research, 60 (2011):S129-S135.
6

Recent Improvements in Geant4 Electromagnetic Physics Models and Interfaces

Ivanchenko, Vladimir; Apostolakis, John; Bagulya, Alexander; Abdelouahed, Haifa Ben; Black, Rachel; Bogdanov, Alexey; Burkhard, Helmut; Chauvie, Stéphane; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Depaola, Gerardo; Di Rosa, Francesco; Elles, Sabine; Francis, Ziad; Grichine, Vladimir; Gumplinger, Peter; Gueye, Paul; Incerti, Sebastien; Ivanchenko, Anton; Jacquemier, Jean; Lechner, Anton; Longo, Francesco; Kadri, Omrane; Karakatsanis, Nicolas; Karamitros, Mathieu; Kokoulin, Rostislav; Kurashige, Hisaya; Maire, Michel; Mantero, Alfonso; Mascialino, Barbara; Moscicki, Jakub; Pandola, Luciano; Perl, Joseph; Petrović, Ivan M.; Ristić-Fira, Aleksandra; Romano, Francesco; Russo, Giorgio; Santin, Giovanni; Schaelicke, Andreas; Toshito, Toshiyuki; Tran, Hoang; Urban, Laszlo; Yamashita, Tomohiro; Zacharatou, Christina

(2011)

TY  - JOUR
AU  - Ivanchenko, Vladimir
AU  - Apostolakis, John
AU  - Bagulya, Alexander
AU  - Abdelouahed, Haifa Ben
AU  - Black, Rachel
AU  - Bogdanov, Alexey
AU  - Burkhard, Helmut
AU  - Chauvie, Stéphane
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Depaola, Gerardo
AU  - Di Rosa, Francesco
AU  - Elles, Sabine
AU  - Francis, Ziad
AU  - Grichine, Vladimir
AU  - Gumplinger, Peter
AU  - Gueye, Paul
AU  - Incerti, Sebastien
AU  - Ivanchenko, Anton
AU  - Jacquemier, Jean
AU  - Lechner, Anton
AU  - Longo, Francesco
AU  - Kadri, Omrane
AU  - Karakatsanis, Nicolas
AU  - Karamitros, Mathieu
AU  - Kokoulin, Rostislav
AU  - Kurashige, Hisaya
AU  - Maire, Michel
AU  - Mantero, Alfonso
AU  - Mascialino, Barbara
AU  - Moscicki, Jakub
AU  - Pandola, Luciano
AU  - Perl, Joseph
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Romano, Francesco
AU  - Russo, Giorgio
AU  - Santin, Giovanni
AU  - Schaelicke, Andreas
AU  - Toshito, Toshiyuki
AU  - Tran, Hoang
AU  - Urban, Laszlo
AU  - Yamashita, Tomohiro
AU  - Zacharatou, Christina
PY  - 2011
UR  - http://inis.iaea.org/search/search.aspx?orig_q=RN:49057834
UR  - http://www.aesj.or.jp/publication/pnst002/data/898-903.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8641
AB  - An overview of the electromagnetic (EM) physics of the Geant4 toolkit is presented Two sets of EM models are available: the 'Standard' initially focused on high energy physics (HEP) while the 'Low-energy' was developed for medical, space and other applications The 'Standard' models provide a faster computation but are less accurate for keV energies, the 'Low-energy' models are more CPU time consuming A common interface to EM physics models has been developed allowing a natural combination of ultra-relativistic, relativistic and low-energy models for the same run providing both precision and CPU performance Due to this migration additional capabilities become available The new developments include relativistic models for bremsstrahlung and e+e- pair production, models of multiple and single scattering, hadron/ion ionization, microdosimetry for very low energies and also improvements in existing Geant4 models In parallel, validation suites and benchmarks have been intensively developed (author)
T2  - Progress in Nuclear Science and Technology
T1  - Recent Improvements in Geant4 Electromagnetic Physics Models and Interfaces
VL  - 2
SP  - 898
EP  - 903
DO  - 10.15669/pnst.2.898
ER  - 
@article{
author = "Ivanchenko, Vladimir and Apostolakis, John and Bagulya, Alexander and Abdelouahed, Haifa Ben and Black, Rachel and Bogdanov, Alexey and Burkhard, Helmut and Chauvie, Stéphane and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Depaola, Gerardo and Di Rosa, Francesco and Elles, Sabine and Francis, Ziad and Grichine, Vladimir and Gumplinger, Peter and Gueye, Paul and Incerti, Sebastien and Ivanchenko, Anton and Jacquemier, Jean and Lechner, Anton and Longo, Francesco and Kadri, Omrane and Karakatsanis, Nicolas and Karamitros, Mathieu and Kokoulin, Rostislav and Kurashige, Hisaya and Maire, Michel and Mantero, Alfonso and Mascialino, Barbara and Moscicki, Jakub and Pandola, Luciano and Perl, Joseph and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Romano, Francesco and Russo, Giorgio and Santin, Giovanni and Schaelicke, Andreas and Toshito, Toshiyuki and Tran, Hoang and Urban, Laszlo and Yamashita, Tomohiro and Zacharatou, Christina",
year = "2011",
abstract = "An overview of the electromagnetic (EM) physics of the Geant4 toolkit is presented Two sets of EM models are available: the 'Standard' initially focused on high energy physics (HEP) while the 'Low-energy' was developed for medical, space and other applications The 'Standard' models provide a faster computation but are less accurate for keV energies, the 'Low-energy' models are more CPU time consuming A common interface to EM physics models has been developed allowing a natural combination of ultra-relativistic, relativistic and low-energy models for the same run providing both precision and CPU performance Due to this migration additional capabilities become available The new developments include relativistic models for bremsstrahlung and e+e- pair production, models of multiple and single scattering, hadron/ion ionization, microdosimetry for very low energies and also improvements in existing Geant4 models In parallel, validation suites and benchmarks have been intensively developed (author)",
journal = "Progress in Nuclear Science and Technology",
title = "Recent Improvements in Geant4 Electromagnetic Physics Models and Interfaces",
volume = "2",
pages = "898-903",
doi = "10.15669/pnst.2.898"
}
Ivanchenko, V., Apostolakis, J., Bagulya, A., Abdelouahed, H. B., Black, R., Bogdanov, A., Burkhard, H., Chauvie, S., Cirrone, G. A. P., Cuttone, G., Depaola, G., Di Rosa, F., Elles, S., Francis, Z., Grichine, V., Gumplinger, P., Gueye, P., Incerti, S., Ivanchenko, A., Jacquemier, J., Lechner, A., Longo, F., Kadri, O., Karakatsanis, N., Karamitros, M., Kokoulin, R., Kurashige, H., Maire, M., Mantero, A., Mascialino, B., Moscicki, J., Pandola, L., Perl, J., Petrović, I. M., Ristić-Fira, A., Romano, F., Russo, G., Santin, G., Schaelicke, A., Toshito, T., Tran, H., Urban, L., Yamashita, T.,& Zacharatou, C.. (2011). Recent Improvements in Geant4 Electromagnetic Physics Models and Interfaces. in Progress in Nuclear Science and Technology, 2, 898-903.
https://doi.org/10.15669/pnst.2.898
Ivanchenko V, Apostolakis J, Bagulya A, Abdelouahed HB, Black R, Bogdanov A, Burkhard H, Chauvie S, Cirrone GAP, Cuttone G, Depaola G, Di Rosa F, Elles S, Francis Z, Grichine V, Gumplinger P, Gueye P, Incerti S, Ivanchenko A, Jacquemier J, Lechner A, Longo F, Kadri O, Karakatsanis N, Karamitros M, Kokoulin R, Kurashige H, Maire M, Mantero A, Mascialino B, Moscicki J, Pandola L, Perl J, Petrović IM, Ristić-Fira A, Romano F, Russo G, Santin G, Schaelicke A, Toshito T, Tran H, Urban L, Yamashita T, Zacharatou C. Recent Improvements in Geant4 Electromagnetic Physics Models and Interfaces. in Progress in Nuclear Science and Technology. 2011;2:898-903.
doi:10.15669/pnst.2.898 .
Ivanchenko, Vladimir, Apostolakis, John, Bagulya, Alexander, Abdelouahed, Haifa Ben, Black, Rachel, Bogdanov, Alexey, Burkhard, Helmut, Chauvie, Stéphane, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Depaola, Gerardo, Di Rosa, Francesco, Elles, Sabine, Francis, Ziad, Grichine, Vladimir, Gumplinger, Peter, Gueye, Paul, Incerti, Sebastien, Ivanchenko, Anton, Jacquemier, Jean, Lechner, Anton, Longo, Francesco, Kadri, Omrane, Karakatsanis, Nicolas, Karamitros, Mathieu, Kokoulin, Rostislav, Kurashige, Hisaya, Maire, Michel, Mantero, Alfonso, Mascialino, Barbara, Moscicki, Jakub, Pandola, Luciano, Perl, Joseph, Petrović, Ivan M., Ristić-Fira, Aleksandra, Romano, Francesco, Russo, Giorgio, Santin, Giovanni, Schaelicke, Andreas, Toshito, Toshiyuki, Tran, Hoang, Urban, Laszlo, Yamashita, Tomohiro, Zacharatou, Christina, "Recent Improvements in Geant4 Electromagnetic Physics Models and Interfaces" in Progress in Nuclear Science and Technology, 2 (2011):898-903,
https://doi.org/10.15669/pnst.2.898 . .
73

Hadrontherapy: a Geant4-Based Tool for Proton/Ion-Therapy Studies

Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Mazzaglia, Enrico S.; Romano, Francesco; Sardina, Daniele; Agodi, Clementina; Attili, Andrea; Blancato, A. Alessandra; De Napoli, Marzio; Di Rosa, Francesco; Kaitaniemi, Pekka; Marchetto, Flavio; Petrović, Ivan M.; Ristić-Fira, Aleksandra; Shin, Jungwook; Tarnavsky, Nikolai; Tropea, Stefania; Zacharatou, Christina

(2011)

TY  - JOUR
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Mazzaglia, Enrico S.
AU  - Romano, Francesco
AU  - Sardina, Daniele
AU  - Agodi, Clementina
AU  - Attili, Andrea
AU  - Blancato, A. Alessandra
AU  - De Napoli, Marzio
AU  - Di Rosa, Francesco
AU  - Kaitaniemi, Pekka
AU  - Marchetto, Flavio
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Shin, Jungwook
AU  - Tarnavsky, Nikolai
AU  - Tropea, Stefania
AU  - Zacharatou, Christina
PY  - 2011
UR  - http://inis.iaea.org/search/search.aspx?orig_q=RN:49052024
UR  - http://www.aesj.or.jp/publication/pnst002/data/207-212.pdf
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8640
AB  - Hadrontherapy is a C++, free and open source application developed using the Geant4 Monte Carlo libraries The basic version of Hadrontherapy is contained in the official Geant4 distribution (wwwcernch/Geant4/download), inside the category of the advanced examples This version permits the simulation of a typical proton/ion transport beam line and the calculation of dose and fluence distributions inside a test phantom A more complete version of the program is separately maintained and released by the authors and it offers a wider set of tools useful for Users interested in proton/ion-therapy studies It gives the possibility to retrieve ion stopping powers in arbitrary geometrical configuration, to calculate 3D distributions of fluences, dose deposited and LET of primary and of the generated secondary beams, to simulate typical nuclear physics experiments, to interactively switch between different implemented geometries, etc In this work the main characteristics of the actual full version of Hadrontherapy will be reported and results discussed and compared with the available experimental data For more information the reader can refer to the Hadrontherapy website (author)
T2  - Progress in Nuclear Science and Technology
T1  - Hadrontherapy: a Geant4-Based Tool for Proton/Ion-Therapy Studies
VL  - 2
SP  - 207
EP  - 212
DO  - 10.15669/pnst.2.207
ER  - 
@article{
author = "Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Mazzaglia, Enrico S. and Romano, Francesco and Sardina, Daniele and Agodi, Clementina and Attili, Andrea and Blancato, A. Alessandra and De Napoli, Marzio and Di Rosa, Francesco and Kaitaniemi, Pekka and Marchetto, Flavio and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Shin, Jungwook and Tarnavsky, Nikolai and Tropea, Stefania and Zacharatou, Christina",
year = "2011",
abstract = "Hadrontherapy is a C++, free and open source application developed using the Geant4 Monte Carlo libraries The basic version of Hadrontherapy is contained in the official Geant4 distribution (wwwcernch/Geant4/download), inside the category of the advanced examples This version permits the simulation of a typical proton/ion transport beam line and the calculation of dose and fluence distributions inside a test phantom A more complete version of the program is separately maintained and released by the authors and it offers a wider set of tools useful for Users interested in proton/ion-therapy studies It gives the possibility to retrieve ion stopping powers in arbitrary geometrical configuration, to calculate 3D distributions of fluences, dose deposited and LET of primary and of the generated secondary beams, to simulate typical nuclear physics experiments, to interactively switch between different implemented geometries, etc In this work the main characteristics of the actual full version of Hadrontherapy will be reported and results discussed and compared with the available experimental data For more information the reader can refer to the Hadrontherapy website (author)",
journal = "Progress in Nuclear Science and Technology",
title = "Hadrontherapy: a Geant4-Based Tool for Proton/Ion-Therapy Studies",
volume = "2",
pages = "207-212",
doi = "10.15669/pnst.2.207"
}
Cirrone, G. A. P., Cuttone, G., Mazzaglia, E. S., Romano, F., Sardina, D., Agodi, C., Attili, A., Blancato, A. A., De Napoli, M., Di Rosa, F., Kaitaniemi, P., Marchetto, F., Petrović, I. M., Ristić-Fira, A., Shin, J., Tarnavsky, N., Tropea, S.,& Zacharatou, C.. (2011). Hadrontherapy: a Geant4-Based Tool for Proton/Ion-Therapy Studies. in Progress in Nuclear Science and Technology, 2, 207-212.
https://doi.org/10.15669/pnst.2.207
Cirrone GAP, Cuttone G, Mazzaglia ES, Romano F, Sardina D, Agodi C, Attili A, Blancato AA, De Napoli M, Di Rosa F, Kaitaniemi P, Marchetto F, Petrović IM, Ristić-Fira A, Shin J, Tarnavsky N, Tropea S, Zacharatou C. Hadrontherapy: a Geant4-Based Tool for Proton/Ion-Therapy Studies. in Progress in Nuclear Science and Technology. 2011;2:207-212.
doi:10.15669/pnst.2.207 .
Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Mazzaglia, Enrico S., Romano, Francesco, Sardina, Daniele, Agodi, Clementina, Attili, Andrea, Blancato, A. Alessandra, De Napoli, Marzio, Di Rosa, Francesco, Kaitaniemi, Pekka, Marchetto, Flavio, Petrović, Ivan M., Ristić-Fira, Aleksandra, Shin, Jungwook, Tarnavsky, Nikolai, Tropea, Stefania, Zacharatou, Christina, "Hadrontherapy: a Geant4-Based Tool for Proton/Ion-Therapy Studies" in Progress in Nuclear Science and Technology, 2 (2011):207-212,
https://doi.org/10.15669/pnst.2.207 . .
53

Carbon ion beam as inducer of melanoma cell apoptosis

Žakula, Jelena; Korićanac, Lela; Keta, Otilija D.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra; Petrović, Ivan M.

(Society of Physical Chemists of Serbia, 2010)

TY  - CONF
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9325
AB  - In vitro effect of carbon ions on apoptosis was studied. The human melanoma
HTB140 cells were irradiated with the 62 MeV/u 12C ion beam. Percentage of
apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic
indexes were calculated. The expression of apoptosis-associated proteins, p53, Bax
and Bcl-2 was estimated by Western blot analyses. A dose dependent increase of
apoptosis was revealed, with the maximum value of 17 % after irradiation with 16
Gy, and the apoptotic index of 7.7. Pro-apoptotic effects of carbon ion beams were
confirmed by the detected changes of key regulators of the mitochondrial apoptotic
pathway, the p53 protein expression and the Bax/Bcl-2 ratio.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Carbon ion beam as inducer of melanoma cell apoptosis
ER  - 
@conference{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2010",
abstract = "In vitro effect of carbon ions on apoptosis was studied. The human melanoma
HTB140 cells were irradiated with the 62 MeV/u 12C ion beam. Percentage of
apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic
indexes were calculated. The expression of apoptosis-associated proteins, p53, Bax
and Bcl-2 was estimated by Western blot analyses. A dose dependent increase of
apoptosis was revealed, with the maximum value of 17 % after irradiation with 16
Gy, and the apoptotic index of 7.7. Pro-apoptotic effects of carbon ion beams were
confirmed by the detected changes of key regulators of the mitochondrial apoptotic
pathway, the p53 protein expression and the Bax/Bcl-2 ratio.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Carbon ion beam as inducer of melanoma cell apoptosis"
}
Žakula, J., Korićanac, L., Keta, O. D., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2010). Carbon ion beam as inducer of melanoma cell apoptosis. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
Žakula J, Korićanac L, Keta OD, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Carbon ion beam as inducer of melanoma cell apoptosis. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;..
Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Carbon ion beam as inducer of melanoma cell apoptosis" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010).

Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak

Petrović, Ivan M.; Ristić-Fira, Aleksandra; Todorović, Danijela V.; Korićanac, Lela; Valastro, Lucia; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo

(2010)

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Valastro, Lucia
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2010
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4101
AB  - Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.
T2  - International Journal of Radiation Biology
T1  - Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak
VL  - 86
IS  - 9
SP  - 742
EP  - 751
DO  - 10.3109/09553002.2010.481322
ER  - 
@article{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Valastro, Lucia and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2010",
abstract = "Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.",
journal = "International Journal of Radiation Biology",
title = "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak",
volume = "86",
number = "9",
pages = "742-751",
doi = "10.3109/09553002.2010.481322"
}
Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Korićanac, L., Valastro, L., Cirrone, G. A. P.,& Cuttone, G.. (2010). Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology, 86(9), 742-751.
https://doi.org/10.3109/09553002.2010.481322
Petrović IM, Ristić-Fira A, Todorović DV, Korićanac L, Valastro L, Cirrone GAP, Cuttone G. Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology. 2010;86(9):742-751.
doi:10.3109/09553002.2010.481322 .
Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Valastro, Lucia, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak" in International Journal of Radiation Biology, 86, no. 9 (2010):742-751,
https://doi.org/10.3109/09553002.2010.481322 . .
33
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Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab

Korićanac, Lela; Žakula, Jelena; Petrović, Ivan M.; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra

(2010)

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petrović, Ivan M.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2010
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4021
AB  - Background: Metastatic melanoma is one of the most aggressive tumours and is also very resistant to current therapeutic approaches. The aim of this investigation was the in vitro study of the anti-proliferative effects of fotemustine (FM; 100 and 250 mu M), bevacizumab (5 mu g/ml) and proton irradiation (12 and 16 Gy) on resistant HTB140 human melanoma cells. Methods: Viability was estimated by sulphorhodamine B assay, while cell proliferation was analyzed by 5-bromo-2-deoxyuridine assay. Cell cycle distribution and apoptosis were examined using flow cytometry. Results: Cell viability and proliferation were reduced after all applied treatments. The level of apoptosis significantly increased after treatment with FM, protons or a combination of all agents, while the apoptotic index ranged from 1.2 to 9.2. Proton irradiation, as well as combined treatment with bevacizumab and protons or 100 mu M FM, bevacizumab and protons, have reduced melanoma cell proliferation through the induction of G1 phase arrest. Single FM (250 mu M) or bevacizumab treatment and their combination, as well as the joint application of these 2 agents with protons, reduced cell proliferation and provoked G2 phase accumulation. Conclusion: The analyzed treatments reduced cell viability and proliferation, triggered G1 or G2 cell cycle phase accumulation and stimulated apoptotic cell death. Copyright (C) 2010 S. Karger AG, Basel
T2  - Chemotherapy
T1  - Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab
VL  - 56
IS  - 3
SP  - 214
EP  - 222
DO  - 10.1159/000316333
ER  - 
@article{
author = "Korićanac, Lela and Žakula, Jelena and Petrović, Ivan M. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2010",
abstract = "Background: Metastatic melanoma is one of the most aggressive tumours and is also very resistant to current therapeutic approaches. The aim of this investigation was the in vitro study of the anti-proliferative effects of fotemustine (FM; 100 and 250 mu M), bevacizumab (5 mu g/ml) and proton irradiation (12 and 16 Gy) on resistant HTB140 human melanoma cells. Methods: Viability was estimated by sulphorhodamine B assay, while cell proliferation was analyzed by 5-bromo-2-deoxyuridine assay. Cell cycle distribution and apoptosis were examined using flow cytometry. Results: Cell viability and proliferation were reduced after all applied treatments. The level of apoptosis significantly increased after treatment with FM, protons or a combination of all agents, while the apoptotic index ranged from 1.2 to 9.2. Proton irradiation, as well as combined treatment with bevacizumab and protons or 100 mu M FM, bevacizumab and protons, have reduced melanoma cell proliferation through the induction of G1 phase arrest. Single FM (250 mu M) or bevacizumab treatment and their combination, as well as the joint application of these 2 agents with protons, reduced cell proliferation and provoked G2 phase accumulation. Conclusion: The analyzed treatments reduced cell viability and proliferation, triggered G1 or G2 cell cycle phase accumulation and stimulated apoptotic cell death. Copyright (C) 2010 S. Karger AG, Basel",
journal = "Chemotherapy",
title = "Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab",
volume = "56",
number = "3",
pages = "214-222",
doi = "10.1159/000316333"
}
Korićanac, L., Žakula, J., Petrović, I. M., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Ristić-Fira, A.. (2010). Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab. in Chemotherapy, 56(3), 214-222.
https://doi.org/10.1159/000316333
Korićanac L, Žakula J, Petrović IM, Valastro LM, Cirrone GAP, Cuttone G, Ristić-Fira A. Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab. in Chemotherapy. 2010;56(3):214-222.
doi:10.1159/000316333 .
Korićanac, Lela, Žakula, Jelena, Petrović, Ivan M., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab" in Chemotherapy, 56, no. 3 (2010):214-222,
https://doi.org/10.1159/000316333 . .
3
3
2

Apoptotic ability of carbon ions on a resistant melanoma cell line

Požega, J. J.; Ristić-Fira, Aleksandra; Korićanac, Lela; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Petrović, Ivan M.

(2008)

TY  - CONF
AU  - Požega, J. J.
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2008
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6761
C3  - FEBS Journal
T1  - Apoptotic ability of carbon ions on a resistant melanoma cell line
VL  - 275
SP  - 425
EP  - 425
ER  - 
@conference{
author = "Požega, J. J. and Ristić-Fira, Aleksandra and Korićanac, Lela and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2008",
journal = "FEBS Journal",
title = "Apoptotic ability of carbon ions on a resistant melanoma cell line",
volume = "275",
pages = "425-425"
}
Požega, J. J., Ristić-Fira, A., Korićanac, L., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Petrović, I. M.. (2008). Apoptotic ability of carbon ions on a resistant melanoma cell line. in FEBS Journal, 275, 425-425.
Požega JJ, Ristić-Fira A, Korićanac L, Valastro LM, Cirrone GAP, Cuttone G, Petrović IM. Apoptotic ability of carbon ions on a resistant melanoma cell line. in FEBS Journal. 2008;275:425-425..
Požega, J. J., Ristić-Fira, Aleksandra, Korićanac, Lela, Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Petrović, Ivan M., "Apoptotic ability of carbon ions on a resistant melanoma cell line" in FEBS Journal, 275 (2008):425-425.

Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons

Korićanac, Lela; Petrović, Ivan M.; Požega, J. J.; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra

(2008)

TY  - CONF
AU  - Korićanac, Lela
AU  - Petrović, Ivan M.
AU  - Požega, J. J.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2008
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6760
C3  - FEBS Journal
T1  - Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons
VL  - 275
SP  - 409
EP  - 409
ER  - 
@conference{
author = "Korićanac, Lela and Petrović, Ivan M. and Požega, J. J. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2008",
journal = "FEBS Journal",
title = "Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons",
volume = "275",
pages = "409-409"
}
Korićanac, L., Petrović, I. M., Požega, J. J., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Ristić-Fira, A.. (2008). Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons. in FEBS Journal, 275, 409-409.
Korićanac L, Petrović IM, Požega JJ, Valastro LM, Cirrone GAP, Cuttone G, Ristić-Fira A. Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons. in FEBS Journal. 2008;275:409-409..
Korićanac, Lela, Petrović, Ivan M., Požega, J. J., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons" in FEBS Journal, 275 (2008):409-409.

Response of a human melanoma cell line to low and high ionizing radiation

Ristić-Fira, Aleksandra; Todorović, Danijela V.; Korićanac, Lela; Petrović, Ivan M.; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Raffaele, Luigi; Cuttone, Giacomo

(2007)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Petrović, Ivan M.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Raffaele, Luigi
AU  - Cuttone, Giacomo
PY  - 2007
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6648
AB  - Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cells decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells.
T2  - Annals of the New York Academy of Sciences
T1  - Response of a human melanoma cell line to low and high ionizing radiation
VL  - 1095
SP  - 165
EP  - 174
DO  - 10.1196/annals.1397.020
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Petrović, Ivan M. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Raffaele, Luigi and Cuttone, Giacomo",
year = "2007",
abstract = "Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cells decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells.",
journal = "Annals of the New York Academy of Sciences",
title = "Response of a human melanoma cell line to low and high ionizing radiation",
volume = "1095",
pages = "165-174",
doi = "10.1196/annals.1397.020"
}
Ristić-Fira, A., Todorović, D. V., Korićanac, L., Petrović, I. M., Valastro, L. M., Cirrone, G. A. P., Raffaele, L.,& Cuttone, G.. (2007). Response of a human melanoma cell line to low and high ionizing radiation. in Annals of the New York Academy of Sciences, 1095, 165-174.
https://doi.org/10.1196/annals.1397.020
Ristić-Fira A, Todorović DV, Korićanac L, Petrović IM, Valastro LM, Cirrone GAP, Raffaele L, Cuttone G. Response of a human melanoma cell line to low and high ionizing radiation. in Annals of the New York Academy of Sciences. 2007;1095:165-174.
doi:10.1196/annals.1397.020 .
Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Petrović, Ivan M., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Raffaele, Luigi, Cuttone, Giacomo, "Response of a human melanoma cell line to low and high ionizing radiation" in Annals of the New York Academy of Sciences, 1095 (2007):165-174,
https://doi.org/10.1196/annals.1397.020 . .
22
19
24

Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam

Petrović, Ivan M.; Ristić-Fira, Aleksandra; Todorović, Danijela V.; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo

(2006)

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2006
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3017
AB  - Purpose: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. Materials and methods: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with Co-60 gamma-rays with single doses, ranging from 8-24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. Results: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of similar to 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. Conclusions: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.
T2  - International Journal of Radiation Biology
T1  - Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam
VL  - 82
IS  - 4
SP  - 251
EP  - 265
DO  - 10.1080/09553000600669859
ER  - 
@article{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2006",
abstract = "Purpose: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. Materials and methods: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with Co-60 gamma-rays with single doses, ranging from 8-24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. Results: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of similar to 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. Conclusions: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.",
journal = "International Journal of Radiation Biology",
title = "Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam",
volume = "82",
number = "4",
pages = "251-265",
doi = "10.1080/09553000600669859"
}
Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Valastro, L. M., Cirrone, G. A. P.,& Cuttone, G.. (2006). Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam. in International Journal of Radiation Biology, 82(4), 251-265.
https://doi.org/10.1080/09553000600669859
Petrović IM, Ristić-Fira A, Todorović DV, Valastro LM, Cirrone GAP, Cuttone G. Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam. in International Journal of Radiation Biology. 2006;82(4):251-265.
doi:10.1080/09553000600669859 .
Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam" in International Journal of Radiation Biology, 82, no. 4 (2006):251-265,
https://doi.org/10.1080/09553000600669859 . .
3
33
35
35

Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays

Ristić-Fira, Aleksandra; Petrović, Ivan M.; Todorović, Danijela V.; Korićanac, Lela; Vujčić, Miroslava T.; Demajo, Miroslav; Sabini, G; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo

(2004)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Vujčić, Miroslava T.
AU  - Demajo, Miroslav
AU  - Sabini, G
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2004
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/2829
AB  - The effects of single irradiation with gamma rays and protons on HTB63 human melanoma cell growth were compared. The exponentially growing cells were irradiated with gamma rays or protons using doses ranging from 2-20 Gy. At 48 It of post-irradiation incubation under standard conditions, cell survival and induction of apoptotic cell death were examined. The best effect of the single irradiation with gamma rays was the reduction of cell growth by up to 26% (p=0.048, irradiation vs. control), obtained using the dose of 16 Gy. The same doses of proton irradiation, having energy at the target of 22.6 MeV, significantly inhibited melanoma cell growth. Doses of 12 and 16 Gy of protons provoked growth inhibition of 48.9% (p=0.003, irradiation vs. control) and 51.2% (p=0.012, irradiation vs. control) respectively. Irradiation with 12 and 16 Gy protons, compared to the effects of the same doses of gamma rays, significantly reduced melanoma cell growth (p=0.015 and p=0.028, protons vs. gamma rays, respectively). Estimated RBEs for growth inhibition of HTB63 cells ranged from 1.02 to 1.45. The electrophoretical analyses of DNA samples and flow cytometric evaluation have shown a low percentage of apoptotic cells after both types of irradiation. The better inhibitory effect achieved by protons in contrast to gamma rays, can be explained considering specific physical properties of protons, especially taking into account the highly localized energy deposition (high LET).
T2  - Oncology Reports
T1  - Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays
VL  - 12
IS  - 6
SP  - 1323
EP  - 1328
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Petrović, Ivan M. and Todorović, Danijela V. and Korićanac, Lela and Vujčić, Miroslava T. and Demajo, Miroslav and Sabini, G and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2004",
abstract = "The effects of single irradiation with gamma rays and protons on HTB63 human melanoma cell growth were compared. The exponentially growing cells were irradiated with gamma rays or protons using doses ranging from 2-20 Gy. At 48 It of post-irradiation incubation under standard conditions, cell survival and induction of apoptotic cell death were examined. The best effect of the single irradiation with gamma rays was the reduction of cell growth by up to 26% (p=0.048, irradiation vs. control), obtained using the dose of 16 Gy. The same doses of proton irradiation, having energy at the target of 22.6 MeV, significantly inhibited melanoma cell growth. Doses of 12 and 16 Gy of protons provoked growth inhibition of 48.9% (p=0.003, irradiation vs. control) and 51.2% (p=0.012, irradiation vs. control) respectively. Irradiation with 12 and 16 Gy protons, compared to the effects of the same doses of gamma rays, significantly reduced melanoma cell growth (p=0.015 and p=0.028, protons vs. gamma rays, respectively). Estimated RBEs for growth inhibition of HTB63 cells ranged from 1.02 to 1.45. The electrophoretical analyses of DNA samples and flow cytometric evaluation have shown a low percentage of apoptotic cells after both types of irradiation. The better inhibitory effect achieved by protons in contrast to gamma rays, can be explained considering specific physical properties of protons, especially taking into account the highly localized energy deposition (high LET).",
journal = "Oncology Reports",
title = "Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays",
volume = "12",
number = "6",
pages = "1323-1328"
}
Ristić-Fira, A., Petrović, I. M., Todorović, D. V., Korićanac, L., Vujčić, M. T., Demajo, M., Sabini, G., Cirrone, G. A. P.,& Cuttone, G.. (2004). Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays. in Oncology Reports, 12(6), 1323-1328.
Ristić-Fira A, Petrović IM, Todorović DV, Korićanac L, Vujčić MT, Demajo M, Sabini G, Cirrone GAP, Cuttone G. Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays. in Oncology Reports. 2004;12(6):1323-1328..
Ristić-Fira, Aleksandra, Petrović, Ivan M., Todorović, Danijela V., Korićanac, Lela, Vujčić, Miroslava T., Demajo, Miroslav, Sabini, G, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays" in Oncology Reports, 12, no. 6 (2004):1323-1328.
5

Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells

Ristić-Fira, Aleksandra; Todorović, Danijela V.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Valastro, Lucia M.; Petrović, Ivan

(Society of Physical Chemists of Serbia, 2004)

TY  - CONF
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Valastro, Lucia M.
AU  - Petrović, Ivan
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9507
AB  - The objective of this study was to determine whether apoptosis and cell cycle redistribution were influenced by high-LET irradiation. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam, within the Bragg peak, delivered over the single dose range from 8 Gy to 24 Gy. At 6 h post-irradiation, there was a low level of early apoptosis. At 48 h irradiated cells were more damaged, showing the increase in number of apoptotic nuclei. The dose dependent cell cycle phase distribution was detected at 48 h post-irradiation. The cell population exhibited phase redistribution toward G2/M phase.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells
VL  - 1
SP  - 429
EP  - 431
ER  - 
@conference{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Valastro, Lucia M. and Petrović, Ivan",
year = "2004",
abstract = "The objective of this study was to determine whether apoptosis and cell cycle redistribution were influenced by high-LET irradiation. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam, within the Bragg peak, delivered over the single dose range from 8 Gy to 24 Gy. At 6 h post-irradiation, there was a low level of early apoptosis. At 48 h irradiated cells were more damaged, showing the increase in number of apoptotic nuclei. The dose dependent cell cycle phase distribution was detected at 48 h post-irradiation. The cell population exhibited phase redistribution toward G2/M phase.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells",
volume = "1",
pages = "429-431"
}
Ristić-Fira, A., Todorović, D. V., Cirrone, G. A. P., Cuttone, G., Valastro, L. M.,& Petrović, I.. (2004). Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 429-431.
Ristić-Fira A, Todorović DV, Cirrone GAP, Cuttone G, Valastro LM, Petrović I. Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:429-431..
Ristić-Fira, Aleksandra, Todorović, Danijela V., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Valastro, Lucia M., Petrović, Ivan, "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):429-431.

Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells

Petrović, Ivan; Todorović, Danijela V.; Valastro, Lucia M.; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Ristić-Fira, Aleksandra

(Society of Physical Chemists of Serbia, 2004)

TY  - CONF
AU  - Petrović, Ivan
AU  - Todorović, Danijela V.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9506
AB  - The aim of this study was to determine the initial inactivation of cells induced by high-energy proton beam designed for the treatment of eye melanoma. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam delivered over the single dose range from 8 Gy to 24 Gy. Position of samples was in the zone of the Bragg peak, having high LET values. Surviving fractions were evaluated at 6, 24 and 48 h post-irradiation. The survival curves exhibited a well-known shoulder, decreasing for doses higher than 8 Gy. Therefore, a significant dose dependent early cell inactivation after single delivery of 16 Gy to 24 Gy to the cell monolayer was observed. With the increase of the post-irradiation incubation time, a better killing effect, as the consequence of clonogenic survival, was detected.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells
VL  - 1
SP  - 426
EP  - 428
ER  - 
@conference{
author = "Petrović, Ivan and Todorović, Danijela V. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2004",
abstract = "The aim of this study was to determine the initial inactivation of cells induced by high-energy proton beam designed for the treatment of eye melanoma. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam delivered over the single dose range from 8 Gy to 24 Gy. Position of samples was in the zone of the Bragg peak, having high LET values. Surviving fractions were evaluated at 6, 24 and 48 h post-irradiation. The survival curves exhibited a well-known shoulder, decreasing for doses higher than 8 Gy. Therefore, a significant dose dependent early cell inactivation after single delivery of 16 Gy to 24 Gy to the cell monolayer was observed. With the increase of the post-irradiation incubation time, a better killing effect, as the consequence of clonogenic survival, was detected.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells",
volume = "1",
pages = "426-428"
}
Petrović, I., Todorović, D. V., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Ristić-Fira, A.. (2004). Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 426-428.
Petrović I, Todorović DV, Valastro LM, Cirrone GAP, Cuttone G, Ristić-Fira A. Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:426-428..
Petrović, Ivan, Todorović, Danijela V., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):426-428.

Inhibition of human melanoma cell growth by proton irradiation

Ristić-Fira, Aleksandra; Todorović, Danijela V.; Petrović, Ivan M.; Ruzvdijic, S; Raffaele, L; Sabini, MG; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Farruggia, G; Masotti, L; Kanazir, Dušan T.

(2001)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Petrović, Ivan M.
AU  - Ruzvdijic, S
AU  - Raffaele, L
AU  - Sabini, MG
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Farruggia, G
AU  - Masotti, L
AU  - Kanazir, Dušan T.
PY  - 2001
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6321
AB  - The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.
T2  - Physica Medica
T1  - Inhibition of human melanoma cell growth by proton irradiation
VL  - 17
SP  - 71
EP  - 75
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Petrović, Ivan M. and Ruzvdijic, S and Raffaele, L and Sabini, MG and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Farruggia, G and Masotti, L and Kanazir, Dušan T.",
year = "2001",
abstract = "The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.",
journal = "Physica Medica",
title = "Inhibition of human melanoma cell growth by proton irradiation",
volume = "17",
pages = "71-75"
}
Ristić-Fira, A., Todorović, D. V., Petrović, I. M., Ruzvdijic, S., Raffaele, L., Sabini, M., Cirrone, G. A. P., Cuttone, G., Farruggia, G., Masotti, L.,& Kanazir, D. T.. (2001). Inhibition of human melanoma cell growth by proton irradiation. in Physica Medica, 17, 71-75.
Ristić-Fira A, Todorović DV, Petrović IM, Ruzvdijic S, Raffaele L, Sabini M, Cirrone GAP, Cuttone G, Farruggia G, Masotti L, Kanazir DT. Inhibition of human melanoma cell growth by proton irradiation. in Physica Medica. 2001;17:71-75..
Ristić-Fira, Aleksandra, Todorović, Danijela V., Petrović, Ivan M., Ruzvdijic, S, Raffaele, L, Sabini, MG, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Farruggia, G, Masotti, L, Kanazir, Dušan T., "Inhibition of human melanoma cell growth by proton irradiation" in Physica Medica, 17 (2001):71-75.
1

The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line

Ristić-Fira, Aleksandra; Nikolic, D; Petrović, Ivan M.; Ruždijić, Sabera; Raffaele, L; Sabini, MG; Cirrone, Giuseppe Antonio Pablo; Cuttone, Giacomo; Farruggia, G; Masotti, L; Kanazir, Dušan T.

(2001)

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Nikolic, D
AU  - Petrović, Ivan M.
AU  - Ruždijić, Sabera
AU  - Raffaele, L
AU  - Sabini, MG
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Farruggia, G
AU  - Masotti, L
AU  - Kanazir, Dušan T.
PY  - 2001
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/2426
AB  - The aim of this work is the in vitro study of the late effects of single proton irradiation on HTB63 human melanoma cell growth, cell cycle and cell death. The experimental conditions were focused on analyzing the effects of irradiation on the periphery of tumour that can be, in clinical practice, close to critical organs. Confluent cell monolayers were irradiated with single doses ranging from 1 - 20 Gy, using proton beams having an energy of 22.6 MeV at the target. Antiproliferative effect of protons, cell cycle analysis and initiation of cell death, were followed 48 hours after irradiation. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analysis and cell viability have shown the G2/M and G1/G0 arrest of irradiated cells correlating with the increase of the applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. These data demonstrate that irradiation with protons, under the chosen experimental conditions, have significant effects on melanoma cell growth inhibition being dose dependent, G2/M cell cycle arrest and appearance of apoptotic nuclei, even 48 hours after irradiation. The results obtained may help the understanding of the relationship between cell proliferation, death and cell cycle regulation of melanomas after proton irradiation.
T2  - Journal of Experimental and Clinical Cancer Research
T1  - The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line
VL  - 20
IS  - 1
SP  - 135
EP  - 143
ER  - 
@article{
author = "Ristić-Fira, Aleksandra and Nikolic, D and Petrović, Ivan M. and Ruždijić, Sabera and Raffaele, L and Sabini, MG and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Farruggia, G and Masotti, L and Kanazir, Dušan T.",
year = "2001",
abstract = "The aim of this work is the in vitro study of the late effects of single proton irradiation on HTB63 human melanoma cell growth, cell cycle and cell death. The experimental conditions were focused on analyzing the effects of irradiation on the periphery of tumour that can be, in clinical practice, close to critical organs. Confluent cell monolayers were irradiated with single doses ranging from 1 - 20 Gy, using proton beams having an energy of 22.6 MeV at the target. Antiproliferative effect of protons, cell cycle analysis and initiation of cell death, were followed 48 hours after irradiation. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analysis and cell viability have shown the G2/M and G1/G0 arrest of irradiated cells correlating with the increase of the applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. These data demonstrate that irradiation with protons, under the chosen experimental conditions, have significant effects on melanoma cell growth inhibition being dose dependent, G2/M cell cycle arrest and appearance of apoptotic nuclei, even 48 hours after irradiation. The results obtained may help the understanding of the relationship between cell proliferation, death and cell cycle regulation of melanomas after proton irradiation.",
journal = "Journal of Experimental and Clinical Cancer Research",
title = "The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line",
volume = "20",
number = "1",
pages = "135-143"
}
Ristić-Fira, A., Nikolic, D., Petrović, I. M., Ruždijić, S., Raffaele, L., Sabini, M., Cirrone, G. A. P., Cuttone, G., Farruggia, G., Masotti, L.,& Kanazir, D. T.. (2001). The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line. in Journal of Experimental and Clinical Cancer Research, 20(1), 135-143.
Ristić-Fira A, Nikolic D, Petrović IM, Ruždijić S, Raffaele L, Sabini M, Cirrone GAP, Cuttone G, Farruggia G, Masotti L, Kanazir DT. The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line. in Journal of Experimental and Clinical Cancer Research. 2001;20(1):135-143..
Ristić-Fira, Aleksandra, Nikolic, D, Petrović, Ivan M., Ruždijić, Sabera, Raffaele, L, Sabini, MG, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Farruggia, G, Masotti, L, Kanazir, Dušan T., "The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line" in Journal of Experimental and Clinical Cancer Research, 20, no. 1 (2001):135-143.
10