TY  - JOUR
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović-Borović, Milica
AU  - Borisev, Ivana
AU  - Novaković, Mirjana M.
AU  - Rakočević, Zlatko Lj.
AU  - Đorđević, Aleksandar N.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8649
AB  - Fullerenol C60(OH)24 with its spherical shape, symmetrical structure, 1 nm size and the ability to form polyionic nanoparticles in water solution, was used to synthesise a novel nanocomposite made of fullerenol nanoparticles (FNP) and iron ions (Fe2+). The FNP/Fe2+ nanocomposite was characterised by DLS and TEM-EDS analyses which have shown that the size distribution of FNP/Fe2+ stayed in the same scope as the size distribution of FNP, ranging from 11 to 60 nm. However, Fe2+ did affect the change of FNP’s zeta potential (− 49.2 mV), shifting it to more positive values (− 30.8 mV). In this study, it was assumed that FNP/Fe2+ could reduce the toxic effects of doxorubicin (Dox). Male Wistar rats were treated i.p. with FNP/Fe2+ nanocomposite 1 h prior to Dox treatment. At the subcellular level, the ultrastructural analysis revealed minor alterations sporadically displayed within the heart and liver tissues. Moreover, at the molecular level, the gene expressions analysis of mRNAs for catalase (heart and liver) and MnSOD (only liver) were significantly downregulated, indicating reduction in oxidative stress. Overall, the pretreatment with FNP/Fe2+ nanocomposite, followed by Dox application, significantly diminished harmful effects of the applied drug on the heart and liver, suggesting the potential protective effect of the nanocomposite on the healthy tissues. © 2019, Springer Nature B.V.
T2  - Journal of Nanoparticle Research
T1  - Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity
VL  - 21
IS  - 11
SP  - 239
DO  - 10.1007/s11051-019-4681-4
ER  - 

@article{
author = "Seke, Mariana and Petrović, Danijela and Labudović-Borović, Milica and Borisev, Ivana and Novaković, Mirjana M. and Rakočević, Zlatko Lj. and Đorđević, Aleksandar N.",
year = "2019",
abstract = "Fullerenol C60(OH)24 with its spherical shape, symmetrical structure, 1 nm size and the ability to form polyionic nanoparticles in water solution, was used to synthesise a novel nanocomposite made of fullerenol nanoparticles (FNP) and iron ions (Fe2+). The FNP/Fe2+ nanocomposite was characterised by DLS and TEM-EDS analyses which have shown that the size distribution of FNP/Fe2+ stayed in the same scope as the size distribution of FNP, ranging from 11 to 60 nm. However, Fe2+ did affect the change of FNP’s zeta potential (− 49.2 mV), shifting it to more positive values (− 30.8 mV). In this study, it was assumed that FNP/Fe2+ could reduce the toxic effects of doxorubicin (Dox). Male Wistar rats were treated i.p. with FNP/Fe2+ nanocomposite 1 h prior to Dox treatment. At the subcellular level, the ultrastructural analysis revealed minor alterations sporadically displayed within the heart and liver tissues. Moreover, at the molecular level, the gene expressions analysis of mRNAs for catalase (heart and liver) and MnSOD (only liver) were significantly downregulated, indicating reduction in oxidative stress. Overall, the pretreatment with FNP/Fe2+ nanocomposite, followed by Dox application, significantly diminished harmful effects of the applied drug on the heart and liver, suggesting the potential protective effect of the nanocomposite on the healthy tissues. © 2019, Springer Nature B.V.",
journal = "Journal of Nanoparticle Research",
title = "Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity",
volume = "21",
number = "11",
pages = "239",
doi = "10.1007/s11051-019-4681-4"
}

Seke, M., Petrović, D., Labudović-Borović, M., Borisev, I., Novaković, M. M., Rakočević, Z. Lj.,& Đorđević, A. N.. (2019). Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity. in Journal of Nanoparticle Research, 21(11), 239.
https://doi.org/10.1007/s11051-019-4681-4

Seke M, Petrović D, Labudović-Borović M, Borisev I, Novaković MM, Rakočević ZL, Đorđević AN. Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity. in Journal of Nanoparticle Research. 2019;21(11):239.
doi:10.1007/s11051-019-4681-4 .

Seke, Mariana, Petrović, Danijela, Labudović-Borović, Milica, Borisev, Ivana, Novaković, Mirjana M., Rakočević, Zlatko Lj., Đorđević, Aleksandar N., "Fullerenol/iron nanocomposite diminishes doxorubicin-induced toxicity" in Journal of Nanoparticle Research, 21, no. 11 (2019):239,
https://doi.org/10.1007/s11051-019-4681-4 . .

TY  - CONF
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović Borović, Milica
AU  - Jović, Danica
AU  - Borišev, Ivana
AU  - Kanački, Zdenko
AU  - Žikić, Dragan
AU  - Đorđević, Aleksandar
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12680
AB  - Introduction: Doxorubicin is the most prominent chemotherapeutic, but its clinical use is limited by its severe systemic toxicity. An iron overload aggravates anthracycline toxicity. Fullerenol in aqueous solutions is in the form of polyanionic nanoparticles, serving as a good carrier of positively charged ions, such as Fe2+. Fullerenol’s antioxidant activity has already been proved in different biological systems. The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite on the rat liver as a pretreatment to doxorubicin application. Methods: After the 24h-treatment, adult male Wistar rats were sacrificed and livers were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, gene expression of enzymes involved in antioxidant defense was measured. Results: Ultrastructural analysis revealed that liver tissue was mainly preserved after the nanocomposite was applied prior to doxorubicin. However, the hepatocytes of animals treated with doxorubicin, presented significantly damaged morphology. Apoptosis of hepatocytes and endothelial cells, mitochondria of irregular size and with disruption of cristae, diffuse injury of capillaries were observed. RT-PCR results have shown that treatment with doxorubicin alone significantly increase the mRNA levels of catalase (p=0.008) and superoxide-dismutase (p=0.000003), while the pretreatment with the nanocomposite prior the doxirubicine treatment, dramaticly downregulated the mRNA levels of catalase (p=0.0004) and superoxide-dismutase (p=0.0001). Conclusion: Our results suggest that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin, demonstrated protection to the liver tissue and induced less damage to the hepatocytes in comparison to doxorubicin alone.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity
SP  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12680
ER  - 

@conference{
author = "Seke, Mariana and Petrović, Danijela and Labudović Borović, Milica and Jović, Danica and Borišev, Ivana and Kanački, Zdenko and Žikić, Dragan and Đorđević, Aleksandar",
year = "2017",
abstract = "Introduction: Doxorubicin is the most prominent chemotherapeutic, but its clinical use is limited by its severe systemic toxicity. An iron overload aggravates anthracycline toxicity. Fullerenol in aqueous solutions is in the form of polyanionic nanoparticles, serving as a good carrier of positively charged ions, such as Fe2+. Fullerenol’s antioxidant activity has already been proved in different biological systems. The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite on the rat liver as a pretreatment to doxorubicin application. Methods: After the 24h-treatment, adult male Wistar rats were sacrificed and livers were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, gene expression of enzymes involved in antioxidant defense was measured. Results: Ultrastructural analysis revealed that liver tissue was mainly preserved after the nanocomposite was applied prior to doxorubicin. However, the hepatocytes of animals treated with doxorubicin, presented significantly damaged morphology. Apoptosis of hepatocytes and endothelial cells, mitochondria of irregular size and with disruption of cristae, diffuse injury of capillaries were observed. RT-PCR results have shown that treatment with doxorubicin alone significantly increase the mRNA levels of catalase (p=0.008) and superoxide-dismutase (p=0.000003), while the pretreatment with the nanocomposite prior the doxirubicine treatment, dramaticly downregulated the mRNA levels of catalase (p=0.0004) and superoxide-dismutase (p=0.0001). Conclusion: Our results suggest that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin, demonstrated protection to the liver tissue and induced less damage to the hepatocytes in comparison to doxorubicin alone.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity",
pages = "71-71",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12680"
}

Seke, M., Petrović, D., Labudović Borović, M., Jović, D., Borišev, I., Kanački, Z., Žikić, D.,& Đorđević, A.. (2017). Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 71-71.
https://hdl.handle.net/21.15107/rcub_vinar_12680

Seke M, Petrović D, Labudović Borović M, Jović D, Borišev I, Kanački Z, Žikić D, Đorđević A. Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:71-71.
https://hdl.handle.net/21.15107/rcub_vinar_12680 .

Seke, Mariana, Petrović, Danijela, Labudović Borović, Milica, Jović, Danica, Borišev, Ivana, Kanački, Zdenko, Žikić, Dragan, Đorđević, Aleksandar, "Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):71-71,
https://hdl.handle.net/21.15107/rcub_vinar_12680 .