TY  - JOUR
AU  - Mirković, Marija D.
AU  - Radović, Magdalena
AU  - Stanković, Dalibor M.
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
AU  - Petrović, Djordje
AU  - Mihajlović-Lalić, Ljiljana E.
AU  - Prijović, Željko
AU  - Milanović, Zorana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10169
AB  - Two new Schiff base cobalt(III) ([Co(LH)Cl2], 1) and nickel(II) ([Ni(LH)ClO4], 2) complexes with a diimine-dioxime ligand, (4,9-diaza-3,10-diethyl-3,9-dodecadiene-2,11-dione bis oxime (LH2)), were synthesized and characterized. The compounds were obtained in MeOH from corresponding metal salts and LH2 in molar ratio 1:1 and further characterized by mass spectrometry, IR spectroscopy, electrochemistry, and elemental analysis. Previously reported copper(II) analog ([Cu2(LH)2]·(ClO4)2, 3) was joined to 1 and 2, and the three metal analogs, 1–3, were further investigated in terms of their electrochemical behavior. Binding studies of the complexes with deoxyribonucleic acid (DNA) and human serum albumin (HSA) were carried out using both spectrophotometric and electrochemical methods. All three complexes exhibit binding affinity towards the DNA chain through intercalative interactions. The binding reaction with HSA showed for 1 and 3 decrease in the peak current obtained in the case of complexes before the addition of HSA, while the Ni complex–HSA possesses the same electroactivity as starting complex. The cytotoxicity of LH2 as well as its metal complexes, and cisplatin were evaluated on CT-26 mouse colon carcinoma and human LS174T cancer cell lines employing MTT assay. The copper(II) complex exhibited very promising anticancer activity compared with cisplatin.
T2  - Journal of Coordination Chemistry
T1  - Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity
IS  - 1-2
SP  - 211
EP  - 224
DO  - 10.1080/00958972.2022.2032683
ER  - 

@article{
author = "Mirković, Marija D. and Radović, Magdalena and Stanković, Dalibor M. and Vranješ-Đurić, Sanja and Janković, Drina and Petrović, Djordje and Mihajlović-Lalić, Ljiljana E. and Prijović, Željko and Milanović, Zorana",
year = "2022",
abstract = "Two new Schiff base cobalt(III) ([Co(LH)Cl2], 1) and nickel(II) ([Ni(LH)ClO4], 2) complexes with a diimine-dioxime ligand, (4,9-diaza-3,10-diethyl-3,9-dodecadiene-2,11-dione bis oxime (LH2)), were synthesized and characterized. The compounds were obtained in MeOH from corresponding metal salts and LH2 in molar ratio 1:1 and further characterized by mass spectrometry, IR spectroscopy, electrochemistry, and elemental analysis. Previously reported copper(II) analog ([Cu2(LH)2]·(ClO4)2, 3) was joined to 1 and 2, and the three metal analogs, 1–3, were further investigated in terms of their electrochemical behavior. Binding studies of the complexes with deoxyribonucleic acid (DNA) and human serum albumin (HSA) were carried out using both spectrophotometric and electrochemical methods. All three complexes exhibit binding affinity towards the DNA chain through intercalative interactions. The binding reaction with HSA showed for 1 and 3 decrease in the peak current obtained in the case of complexes before the addition of HSA, while the Ni complex–HSA possesses the same electroactivity as starting complex. The cytotoxicity of LH2 as well as its metal complexes, and cisplatin were evaluated on CT-26 mouse colon carcinoma and human LS174T cancer cell lines employing MTT assay. The copper(II) complex exhibited very promising anticancer activity compared with cisplatin.",
journal = "Journal of Coordination Chemistry",
title = "Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity",
number = "1-2",
pages = "211-224",
doi = "10.1080/00958972.2022.2032683"
}

Mirković, M. D., Radović, M., Stanković, D. M., Vranješ-Đurić, S., Janković, D., Petrović, D., Mihajlović-Lalić, L. E., Prijović, Ž.,& Milanović, Z.. (2022). Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity. in Journal of Coordination Chemistry(1-2), 211-224.
https://doi.org/10.1080/00958972.2022.2032683

Mirković MD, Radović M, Stanković DM, Vranješ-Đurić S, Janković D, Petrović D, Mihajlović-Lalić LE, Prijović Ž, Milanović Z. Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity. in Journal of Coordination Chemistry. 2022;(1-2):211-224.
doi:10.1080/00958972.2022.2032683 .

Mirković, Marija D., Radović, Magdalena, Stanković, Dalibor M., Vranješ-Đurić, Sanja, Janković, Drina, Petrović, Djordje, Mihajlović-Lalić, Ljiljana E., Prijović, Željko, Milanović, Zorana, "Co(III), Ni(II) and Cu(II) complexes with a tetradentate Schiff base ligand: synthesis, characterization, electrochemical behavior, binding assessment and in vitro cytotoxicity" in Journal of Coordination Chemistry, no. 1-2 (2022):211-224,
https://doi.org/10.1080/00958972.2022.2032683 . .

TY  - JOUR
AU  - Mihajlović-Lalić, Ljiljana E.
AU  - Stanković, Dalibor M.
AU  - Novaković, Irena T.
AU  - Grgurić-Šipka, Sanja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10344
AB  - Three ruthenium-bipyridine complexes (1–3) carrying acetylpyridine ligand unit were synthesized in methanol via the reaction of [RuCl2(bpy)2] with corresponding acetylpyridine (2-, 3-, and 4-acpy). Obtained complexes were characterized by (1H and 13C) NMR and IR spectroscopy, MS spectrometry, UV‒vis spectrophotometry, and cyclic voltammetry. Their structural characterization revealed bidentate coordination mode for 2-acpy while 3- and 4-acpy acted as monodentate ligands. The electrochemical profile of newly synthesized compounds was investigated by cyclic voltammetry which confirmed their electrochemical activity. Voltammetric responses within the −1.20 < Ep < 1.50 V range of potentials were summarized in two major events: Ru(II)→Ru(III) oxidation spotted at app. ΔEp = 0.65 V and successive reductions of bpy units located from ‒0.79 V to 0.47 V (vs. Ag/AgCl (3 M) electrode). The DNA-binding activity of the complexes was evaluated by both UV‒vis spectrophotometry and cyclic voltammetry indicating DNA-intercalation with a slight contribution of electrostatic interactions. Furthermore, antimicrobial activity was tested against bacterial and fungal strains, for which moderate activity was observed. Assessment of in vitro toxicity against freshly hatched nauplii of Artemia salina as well as radical scavenging capacity was evaluated. The test compounds showed neither toxicity nor antioxidant activity.
T2  - Journal of Coordination Chemistry
T1  - (Electro)chemical and antimicrobial characterization of novel Ru(II) bipyridine complexes with acetylpyridine analogs
SP  - 1
EP  - 15
DO  - 10.1080/00958972.2022.2090247
ER  - 

@article{
author = "Mihajlović-Lalić, Ljiljana E. and Stanković, Dalibor M. and Novaković, Irena T. and Grgurić-Šipka, Sanja",
year = "2022",
abstract = "Three ruthenium-bipyridine complexes (1–3) carrying acetylpyridine ligand unit were synthesized in methanol via the reaction of [RuCl2(bpy)2] with corresponding acetylpyridine (2-, 3-, and 4-acpy). Obtained complexes were characterized by (1H and 13C) NMR and IR spectroscopy, MS spectrometry, UV‒vis spectrophotometry, and cyclic voltammetry. Their structural characterization revealed bidentate coordination mode for 2-acpy while 3- and 4-acpy acted as monodentate ligands. The electrochemical profile of newly synthesized compounds was investigated by cyclic voltammetry which confirmed their electrochemical activity. Voltammetric responses within the −1.20 < Ep < 1.50 V range of potentials were summarized in two major events: Ru(II)→Ru(III) oxidation spotted at app. ΔEp = 0.65 V and successive reductions of bpy units located from ‒0.79 V to 0.47 V (vs. Ag/AgCl (3 M) electrode). The DNA-binding activity of the complexes was evaluated by both UV‒vis spectrophotometry and cyclic voltammetry indicating DNA-intercalation with a slight contribution of electrostatic interactions. Furthermore, antimicrobial activity was tested against bacterial and fungal strains, for which moderate activity was observed. Assessment of in vitro toxicity against freshly hatched nauplii of Artemia salina as well as radical scavenging capacity was evaluated. The test compounds showed neither toxicity nor antioxidant activity.",
journal = "Journal of Coordination Chemistry",
title = "(Electro)chemical and antimicrobial characterization of novel Ru(II) bipyridine complexes with acetylpyridine analogs",
pages = "1-15",
doi = "10.1080/00958972.2022.2090247"
}

Mihajlović-Lalić, L. E., Stanković, D. M., Novaković, I. T.,& Grgurić-Šipka, S.. (2022). (Electro)chemical and antimicrobial characterization of novel Ru(II) bipyridine complexes with acetylpyridine analogs. in Journal of Coordination Chemistry, 1-15.
https://doi.org/10.1080/00958972.2022.2090247

Mihajlović-Lalić LE, Stanković DM, Novaković IT, Grgurić-Šipka S. (Electro)chemical and antimicrobial characterization of novel Ru(II) bipyridine complexes with acetylpyridine analogs. in Journal of Coordination Chemistry. 2022;:1-15.
doi:10.1080/00958972.2022.2090247 .

Mihajlović-Lalić, Ljiljana E., Stanković, Dalibor M., Novaković, Irena T., Grgurić-Šipka, Sanja, "(Electro)chemical and antimicrobial characterization of novel Ru(II) bipyridine complexes with acetylpyridine analogs" in Journal of Coordination Chemistry (2022):1-15,
https://doi.org/10.1080/00958972.2022.2090247 . .

TY  - JOUR
AU  - Mihajlović-Lalić, Ljiljana E.
AU  - Stanković, Dalibor M.
AU  - Poljarević, Jelena M.
AU  - Sabo, Tibor J.
AU  - Manojlović, Dragan D.
PY  - 2018
UR  - http://jes.ecsdl.org/lookup/doi/10.1149/2.1121810jes
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7892
AB  - The electrochemical behavior of four dialkyl esters (methyl, ethyl, n-propyl and n-butyl) of (S,S)-α,α ′-(1,2-ethanediyldiimino)- bis[cyclohexanepropanoic acid] with moderate antitumor activity has been studied by cyclic voltammetry. I-E curves recorded at boron-doped diamond electrode in -2.00 < Ep < 0.00 V range show a single oxidative peak located in the -0.95 < Ep,a < -0.52 V region in a form of one-electron wave. The electrochemical processes appear to be irreversible for all compounds except for the compound with methyl substituent which demonstrates well-defined reversible electron transfer. Derivatives with bulkier moieties are easier to oxidize. For all investigated compounds an amine-centered redox process is dominant. Further investigation indicates that electrochemical reaction is diffusion controlled, chemically irreversible process. The results are discussed in terms of linking their electrochemical activity to antitumor effect. © 2018 The Electrochemical Society.
T2  - Journal of The Electrochemical Society
T1  - Voltammetric Study of Antitumor Efficient Ethylenediamine-Type of Ligands
VL  - 165
IS  - 10
SP  - G123
EP  - G127
DO  - 10.1149/2.1121810jes
ER  - 

@article{
author = "Mihajlović-Lalić, Ljiljana E. and Stanković, Dalibor M. and Poljarević, Jelena M. and Sabo, Tibor J. and Manojlović, Dragan D.",
year = "2018",
abstract = "The electrochemical behavior of four dialkyl esters (methyl, ethyl, n-propyl and n-butyl) of (S,S)-α,α ′-(1,2-ethanediyldiimino)- bis[cyclohexanepropanoic acid] with moderate antitumor activity has been studied by cyclic voltammetry. I-E curves recorded at boron-doped diamond electrode in -2.00 < Ep < 0.00 V range show a single oxidative peak located in the -0.95 < Ep,a < -0.52 V region in a form of one-electron wave. The electrochemical processes appear to be irreversible for all compounds except for the compound with methyl substituent which demonstrates well-defined reversible electron transfer. Derivatives with bulkier moieties are easier to oxidize. For all investigated compounds an amine-centered redox process is dominant. Further investigation indicates that electrochemical reaction is diffusion controlled, chemically irreversible process. The results are discussed in terms of linking their electrochemical activity to antitumor effect. © 2018 The Electrochemical Society.",
journal = "Journal of The Electrochemical Society",
title = "Voltammetric Study of Antitumor Efficient Ethylenediamine-Type of Ligands",
volume = "165",
number = "10",
pages = "G123-G127",
doi = "10.1149/2.1121810jes"
}

Mihajlović-Lalić, L. E., Stanković, D. M., Poljarević, J. M., Sabo, T. J.,& Manojlović, D. D.. (2018). Voltammetric Study of Antitumor Efficient Ethylenediamine-Type of Ligands. in Journal of The Electrochemical Society, 165(10), G123-G127.
https://doi.org/10.1149/2.1121810jes

Mihajlović-Lalić LE, Stanković DM, Poljarević JM, Sabo TJ, Manojlović DD. Voltammetric Study of Antitumor Efficient Ethylenediamine-Type of Ligands. in Journal of The Electrochemical Society. 2018;165(10):G123-G127.
doi:10.1149/2.1121810jes .

Mihajlović-Lalić, Ljiljana E., Stanković, Dalibor M., Poljarević, Jelena M., Sabo, Tibor J., Manojlović, Dragan D., "Voltammetric Study of Antitumor Efficient Ethylenediamine-Type of Ligands" in Journal of The Electrochemical Society, 165, no. 10 (2018):G123-G127,
https://doi.org/10.1149/2.1121810jes . .

TY  - JOUR
AU  - Baroud, Afya A.
AU  - Mihajlović-Lalić, Ljiljana E.
AU  - Gligorijević, Nevenka N.
AU  - Aranđelović, Sandra
AU  - Stanković, Dalibor M.
AU  - Radulović, Siniša S.
AU  - Van Hecke, Kristof
AU  - Savić, Aleksandar
AU  - Grgurić-Šipka, Sanja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1438
AB  - Complexes 1-4, [Ru(L)(bpy)(2)]PF6, where bpy=2,2-bipyridine; HL=3-methylpyridine-2-carboxylic acid (HL1), 6-methylpyridine-2-carboxylic acid (HL2), 5-bromopyridine-2-carboxylic acid (HL3) and 6-bromopyridine-2-carboxylic acid (HL4), were synthesized and characterized. The electrochemical character of the complexes was investigated by cyclic voltammetry revealing two reversible reduction waves in the negative range of potentials, most likely due to a reduction of the bipyridine moiety. Cytotoxicity studies by MTT assay for 72h of drug action revealed that 2-4 exhibited moderate activity in cervical human tumor cells (HeLa). Complex 2 exhibited low activity in colon cancer LS-174 cells (180 +/- 10), while all complexes were devoid of activity in lung cancer A549 and non-tumor MRC-5 cells, up to 200M. Combinational studies of the most active complex 2, with pharmacological modulators of cell redox status, L-buthionine-sulfoximine (L-BSO) or N-acetyl-L-cysteine (NAC), showed that when L-BSO potentiated, 2 induced a sub-G1 peak of the cell cycle in the HeLa cell line. UV-vis and cyclic voltammetry were performed in order to investigate the binding mode of 2 to DNA and suggested intercalation for the complex-DNA interaction. [GRAPHICS]
T2  - Journal of Coordination Chemistry
T1  - Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation
VL  - 70
IS  - 5
SP  - 831
EP  - 847
DO  - 10.1080/00958972.2017.1282611
ER  - 

@article{
author = "Baroud, Afya A. and Mihajlović-Lalić, Ljiljana E. and Gligorijević, Nevenka N. and Aranđelović, Sandra and Stanković, Dalibor M. and Radulović, Siniša S. and Van Hecke, Kristof and Savić, Aleksandar and Grgurić-Šipka, Sanja",
year = "2017",
abstract = "Complexes 1-4, [Ru(L)(bpy)(2)]PF6, where bpy=2,2-bipyridine; HL=3-methylpyridine-2-carboxylic acid (HL1), 6-methylpyridine-2-carboxylic acid (HL2), 5-bromopyridine-2-carboxylic acid (HL3) and 6-bromopyridine-2-carboxylic acid (HL4), were synthesized and characterized. The electrochemical character of the complexes was investigated by cyclic voltammetry revealing two reversible reduction waves in the negative range of potentials, most likely due to a reduction of the bipyridine moiety. Cytotoxicity studies by MTT assay for 72h of drug action revealed that 2-4 exhibited moderate activity in cervical human tumor cells (HeLa). Complex 2 exhibited low activity in colon cancer LS-174 cells (180 +/- 10), while all complexes were devoid of activity in lung cancer A549 and non-tumor MRC-5 cells, up to 200M. Combinational studies of the most active complex 2, with pharmacological modulators of cell redox status, L-buthionine-sulfoximine (L-BSO) or N-acetyl-L-cysteine (NAC), showed that when L-BSO potentiated, 2 induced a sub-G1 peak of the cell cycle in the HeLa cell line. UV-vis and cyclic voltammetry were performed in order to investigate the binding mode of 2 to DNA and suggested intercalation for the complex-DNA interaction. [GRAPHICS]",
journal = "Journal of Coordination Chemistry",
title = "Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation",
volume = "70",
number = "5",
pages = "831-847",
doi = "10.1080/00958972.2017.1282611"
}

Baroud, A. A., Mihajlović-Lalić, L. E., Gligorijević, N. N., Aranđelović, S., Stanković, D. M., Radulović, S. S., Van Hecke, K., Savić, A.,& Grgurić-Šipka, S.. (2017). Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation. in Journal of Coordination Chemistry, 70(5), 831-847.
https://doi.org/10.1080/00958972.2017.1282611

Baroud AA, Mihajlović-Lalić LE, Gligorijević NN, Aranđelović S, Stanković DM, Radulović SS, Van Hecke K, Savić A, Grgurić-Šipka S. Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation. in Journal of Coordination Chemistry. 2017;70(5):831-847.
doi:10.1080/00958972.2017.1282611 .

Baroud, Afya A., Mihajlović-Lalić, Ljiljana E., Gligorijević, Nevenka N., Aranđelović, Sandra, Stanković, Dalibor M., Radulović, Siniša S., Van Hecke, Kristof, Savić, Aleksandar, Grgurić-Šipka, Sanja, "Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation" in Journal of Coordination Chemistry, 70, no. 5 (2017):831-847,
https://doi.org/10.1080/00958972.2017.1282611 . .

TY  - JOUR
AU  - Baroud, Afya A.
AU  - Mihajlović-Lalić, Ljiljana E.
AU  - Stanković, Dalibor M.
AU  - Kajzerberger, Marijana
AU  - Van Hecke, Kristof
AU  - Grgurić-Šipka, Sanja
AU  - Savić, Aleksandar
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1569
AB  - A new Ru(II) bipyridyl complex with O-4-hydrogenpyridine-2,4-dicarboxylate was synthesized and characterized by IR, NMR and mass spectrometry, X-ray diffraction analysis and elemental analysis. The electrochemical characteristics of the complex were investigated by cyclic voltammetry, revealing Ru(II)/Ru(III) electron transfer in the positive range of potentials. On the opposite potential side, multiple partially reversible peaks were dominant, representing subsequent reductions of the bulky bipyridyl moiety. The cytotoxic activity of the complex was tested in two human cancer cell lines: A549 (lung cancer) and K562 (leukemia) as well as non-tumor MRC-5 cells, by MTT assays. The IC50 values were GT 300 and 177.63+/-2.28 mu M for the A549 and K562 cells, respectively.
T2  - Journal of the Serbian Chemical Society
T1  - New ruthenium(II) bipyridyl complex: Synthesis, crystal structure and cytotoxicity
VL  - 82
IS  - 3
SP  - 267
EP  - 275
DO  - 10.2298/JSC170109025B
ER  - 

@article{
author = "Baroud, Afya A. and Mihajlović-Lalić, Ljiljana E. and Stanković, Dalibor M. and Kajzerberger, Marijana and Van Hecke, Kristof and Grgurić-Šipka, Sanja and Savić, Aleksandar",
year = "2017",
abstract = "A new Ru(II) bipyridyl complex with O-4-hydrogenpyridine-2,4-dicarboxylate was synthesized and characterized by IR, NMR and mass spectrometry, X-ray diffraction analysis and elemental analysis. The electrochemical characteristics of the complex were investigated by cyclic voltammetry, revealing Ru(II)/Ru(III) electron transfer in the positive range of potentials. On the opposite potential side, multiple partially reversible peaks were dominant, representing subsequent reductions of the bulky bipyridyl moiety. The cytotoxic activity of the complex was tested in two human cancer cell lines: A549 (lung cancer) and K562 (leukemia) as well as non-tumor MRC-5 cells, by MTT assays. The IC50 values were GT 300 and 177.63+/-2.28 mu M for the A549 and K562 cells, respectively.",
journal = "Journal of the Serbian Chemical Society",
title = "New ruthenium(II) bipyridyl complex: Synthesis, crystal structure and cytotoxicity",
volume = "82",
number = "3",
pages = "267-275",
doi = "10.2298/JSC170109025B"
}

Baroud, A. A., Mihajlović-Lalić, L. E., Stanković, D. M., Kajzerberger, M., Van Hecke, K., Grgurić-Šipka, S.,& Savić, A.. (2017). New ruthenium(II) bipyridyl complex: Synthesis, crystal structure and cytotoxicity. in Journal of the Serbian Chemical Society, 82(3), 267-275.
https://doi.org/10.2298/JSC170109025B

Baroud AA, Mihajlović-Lalić LE, Stanković DM, Kajzerberger M, Van Hecke K, Grgurić-Šipka S, Savić A. New ruthenium(II) bipyridyl complex: Synthesis, crystal structure and cytotoxicity. in Journal of the Serbian Chemical Society. 2017;82(3):267-275.
doi:10.2298/JSC170109025B .

Baroud, Afya A., Mihajlović-Lalić, Ljiljana E., Stanković, Dalibor M., Kajzerberger, Marijana, Van Hecke, Kristof, Grgurić-Šipka, Sanja, Savić, Aleksandar, "New ruthenium(II) bipyridyl complex: Synthesis, crystal structure and cytotoxicity" in Journal of the Serbian Chemical Society, 82, no. 3 (2017):267-275,
https://doi.org/10.2298/JSC170109025B . .

TY  - JOUR
AU  - Baroud, Afya A.
AU  - Mihajlović-Lalić, Ljiljana E.
AU  - Gligorijević, Nevenka N.
AU  - Aranđelović, Sandra
AU  - Stanković, Dalibor M.
AU  - Radulović, Siniša S.
AU  - Van Hecke, Kristof
AU  - Savić, Aleksandar
AU  - Grgurić-Šipka, Sanja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8852
AB  - Complexes 1-4, [Ru(L)(bpy)(2)]PF6, where bpy=2,2-bipyridine; HL=3-methylpyridine-2-carboxylic acid (HL1), 6-methylpyridine-2-carboxylic acid (HL2), 5-bromopyridine-2-carboxylic acid (HL3) and 6-bromopyridine-2-carboxylic acid (HL4), were synthesized and characterized. The electrochemical character of the complexes was investigated by cyclic voltammetry revealing two reversible reduction waves in the negative range of potentials, most likely due to a reduction of the bipyridine moiety. Cytotoxicity studies by MTT assay for 72h of drug action revealed that 2-4 exhibited moderate activity in cervical human tumor cells (HeLa). Complex 2 exhibited low activity in colon cancer LS-174 cells (180 +/- 10), while all complexes were devoid of activity in lung cancer A549 and non-tumor MRC-5 cells, up to 200M. Combinational studies of the most active complex 2, with pharmacological modulators of cell redox status, L-buthionine-sulfoximine (L-BSO) or N-acetyl-L-cysteine (NAC), showed that when L-BSO potentiated, 2 induced a sub-G1 peak of the cell cycle in the HeLa cell line. UV-vis and cyclic voltammetry were performed in order to investigate the binding mode of 2 to DNA and suggested intercalation for the complex-DNA interaction. [GRAPHICS]
T2  - Journal of Coordination Chemistry
T1  - Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation
VL  - 70
IS  - 5
SP  - 831
EP  - 847
DO  - 10.1080/00958972.2017.1282611
ER  - 

@article{
author = "Baroud, Afya A. and Mihajlović-Lalić, Ljiljana E. and Gligorijević, Nevenka N. and Aranđelović, Sandra and Stanković, Dalibor M. and Radulović, Siniša S. and Van Hecke, Kristof and Savić, Aleksandar and Grgurić-Šipka, Sanja",
year = "2017",
abstract = "Complexes 1-4, [Ru(L)(bpy)(2)]PF6, where bpy=2,2-bipyridine; HL=3-methylpyridine-2-carboxylic acid (HL1), 6-methylpyridine-2-carboxylic acid (HL2), 5-bromopyridine-2-carboxylic acid (HL3) and 6-bromopyridine-2-carboxylic acid (HL4), were synthesized and characterized. The electrochemical character of the complexes was investigated by cyclic voltammetry revealing two reversible reduction waves in the negative range of potentials, most likely due to a reduction of the bipyridine moiety. Cytotoxicity studies by MTT assay for 72h of drug action revealed that 2-4 exhibited moderate activity in cervical human tumor cells (HeLa). Complex 2 exhibited low activity in colon cancer LS-174 cells (180 +/- 10), while all complexes were devoid of activity in lung cancer A549 and non-tumor MRC-5 cells, up to 200M. Combinational studies of the most active complex 2, with pharmacological modulators of cell redox status, L-buthionine-sulfoximine (L-BSO) or N-acetyl-L-cysteine (NAC), showed that when L-BSO potentiated, 2 induced a sub-G1 peak of the cell cycle in the HeLa cell line. UV-vis and cyclic voltammetry were performed in order to investigate the binding mode of 2 to DNA and suggested intercalation for the complex-DNA interaction. [GRAPHICS]",
journal = "Journal of Coordination Chemistry",
title = "Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation",
volume = "70",
number = "5",
pages = "831-847",
doi = "10.1080/00958972.2017.1282611"
}

Baroud, A. A., Mihajlović-Lalić, L. E., Gligorijević, N. N., Aranđelović, S., Stanković, D. M., Radulović, S. S., Van Hecke, K., Savić, A.,& Grgurić-Šipka, S.. (2017). Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation. in Journal of Coordination Chemistry, 70(5), 831-847.
https://doi.org/10.1080/00958972.2017.1282611

Baroud AA, Mihajlović-Lalić LE, Gligorijević NN, Aranđelović S, Stanković DM, Radulović SS, Van Hecke K, Savić A, Grgurić-Šipka S. Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation. in Journal of Coordination Chemistry. 2017;70(5):831-847.
doi:10.1080/00958972.2017.1282611 .

Baroud, Afya A., Mihajlović-Lalić, Ljiljana E., Gligorijević, Nevenka N., Aranđelović, Sandra, Stanković, Dalibor M., Radulović, Siniša S., Van Hecke, Kristof, Savić, Aleksandar, Grgurić-Šipka, Sanja, "Ruthenium(II) bipyridine complexes: from synthesis and crystal structures to electrochemical and cytotoxicity investigation" in Journal of Coordination Chemistry, 70, no. 5 (2017):831-847,
https://doi.org/10.1080/00958972.2017.1282611 . .