TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Jesthke, Anke
AU  - Čolović, Božana M.
AU  - Milovanović, Petar
AU  - Jevremovic, Danimir
AU  - Kisić, Danilo
AU  - vom Scheidt, Annika
AU  - Hahn, Michael
AU  - Amling, Michael
AU  - Jokanović, Vukoman R.
AU  - Busse, Bjoern
AU  - Đurić, Marija
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/847
AB  - Introduction: Calcium silicate cements (CSCs) with the addition of nanohydroxyapatite and calcium carbonate play a critical role in dental applications. To further improve their properties, particularly radiopacity and biointeractivity, the fluoride-containing radiopacifier ytterbium trifluoride (YbF3) was added to their composition, and biological and mechanical characteristics were evaluated. Methods: YbF3 was added to 3 different CSCs: cement I (CSC + calcium carbonate), cement II (CSC + nanohydroxyapatite), and Portland cement. Material characterization encompassed measurements of pH, calcium, ytterbium, and fluoride ion release; radiopacity; setting time; porosity; microindentation properties; wettability; and Fourier transform infrared spectroscopic, x-ray diffraction, and scanning electron microscopic analyses. Osteoblast- and osteoclast-like cells were grown on the materials surface to evaluate their adherence. Results: The addition of calcium carbonate, nanohydroxyapatite, and 30 wt% of YbF3 improved radiopacity and the setting time of experimental cements. The pH values did not differ among the groups. The greatest ytterbium and fluoride releases occurred in the Portland cement + YbF3 group. Combined x-ray diffraction and Fourier transform infrared spectroscopic analysis showed the presence of calcium hydroxide and calcium silicate hydrates. In addition, the presence of calcium ytterbium fluoride and ytterbium oxide proved that YbF3 reacted with cement compounds. Wettability of cement I + YbF3 was superior to other formulations, but its porosity and microindentation properties were weaker than in the Portland cement + YbF3 mixture. Cement II + YbF3 presented micromechanical indentation and porosity characteristics similar to the Portland-based cement formulation. Osteoclast- and osteoblast-like cells adhered to the cements surfaces without alteration of the cell structural integrity. Conclusions: YbF3-containing CSCs with nanostructured hydroxyapatite and calcium carbonate are well suited for dental application.
T2  - Journal of Endodontics
T1  - Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements
VL  - 41
IS  - 12
SP  - 2050
EP  - 2057
DO  - 10.1016/j.joen.2015.09.008
ER  - 

@article{
author = "Antonijević, Đorđe and Jesthke, Anke and Čolović, Božana M. and Milovanović, Petar and Jevremovic, Danimir and Kisić, Danilo and vom Scheidt, Annika and Hahn, Michael and Amling, Michael and Jokanović, Vukoman R. and Busse, Bjoern and Đurić, Marija",
year = "2015",
abstract = "Introduction: Calcium silicate cements (CSCs) with the addition of nanohydroxyapatite and calcium carbonate play a critical role in dental applications. To further improve their properties, particularly radiopacity and biointeractivity, the fluoride-containing radiopacifier ytterbium trifluoride (YbF3) was added to their composition, and biological and mechanical characteristics were evaluated. Methods: YbF3 was added to 3 different CSCs: cement I (CSC + calcium carbonate), cement II (CSC + nanohydroxyapatite), and Portland cement. Material characterization encompassed measurements of pH, calcium, ytterbium, and fluoride ion release; radiopacity; setting time; porosity; microindentation properties; wettability; and Fourier transform infrared spectroscopic, x-ray diffraction, and scanning electron microscopic analyses. Osteoblast- and osteoclast-like cells were grown on the materials surface to evaluate their adherence. Results: The addition of calcium carbonate, nanohydroxyapatite, and 30 wt% of YbF3 improved radiopacity and the setting time of experimental cements. The pH values did not differ among the groups. The greatest ytterbium and fluoride releases occurred in the Portland cement + YbF3 group. Combined x-ray diffraction and Fourier transform infrared spectroscopic analysis showed the presence of calcium hydroxide and calcium silicate hydrates. In addition, the presence of calcium ytterbium fluoride and ytterbium oxide proved that YbF3 reacted with cement compounds. Wettability of cement I + YbF3 was superior to other formulations, but its porosity and microindentation properties were weaker than in the Portland cement + YbF3 mixture. Cement II + YbF3 presented micromechanical indentation and porosity characteristics similar to the Portland-based cement formulation. Osteoclast- and osteoblast-like cells adhered to the cements surfaces without alteration of the cell structural integrity. Conclusions: YbF3-containing CSCs with nanostructured hydroxyapatite and calcium carbonate are well suited for dental application.",
journal = "Journal of Endodontics",
title = "Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements",
volume = "41",
number = "12",
pages = "2050-2057",
doi = "10.1016/j.joen.2015.09.008"
}

Antonijević, Đ., Jesthke, A., Čolović, B. M., Milovanović, P., Jevremovic, D., Kisić, D., vom Scheidt, A., Hahn, M., Amling, M., Jokanović, V. R., Busse, B.,& Đurić, M.. (2015). Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements. in Journal of Endodontics, 41(12), 2050-2057.
https://doi.org/10.1016/j.joen.2015.09.008

Antonijević Đ, Jesthke A, Čolović BM, Milovanović P, Jevremovic D, Kisić D, vom Scheidt A, Hahn M, Amling M, Jokanović VR, Busse B, Đurić M. Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements. in Journal of Endodontics. 2015;41(12):2050-2057.
doi:10.1016/j.joen.2015.09.008 .

Antonijević, Đorđe, Jesthke, Anke, Čolović, Božana M., Milovanović, Petar, Jevremovic, Danimir, Kisić, Danilo, vom Scheidt, Annika, Hahn, Michael, Amling, Michael, Jokanović, Vukoman R., Busse, Bjoern, Đurić, Marija, "Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements" in Journal of Endodontics, 41, no. 12 (2015):2050-2057,
https://doi.org/10.1016/j.joen.2015.09.008 . .

TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Milovanović, Petar
AU  - Brajkovic, Denis
AU  - Ilić, Dragan V.
AU  - Hahn, Michael
AU  - Amling, Michael
AU  - Rakočević, Zlatko Lj.
AU  - Đurić, Marija
AU  - Busse, Bjoern
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/791
AB  - The objective of this study was to determine the effects of various irrigation solutions on root canal dentine and gutta-percha surface properties. In addition, the effects of disinfectant chemicals on the wettability and surface morphological properties of the filling materials were evaluated. Ethylenediaminetetraacetic acid (EDTA), citric acid, and ozone were employed as irrigation solutions for dentine and gutta-percha treatment. Thereafter, the samples microstructure, degree of mineralization, and mechanical properties were assessed by means of quantitative backscattered electron imaging (qBEI) and reference point indentation (RN). A contact angle analyzer was used to measure adhesion on the tested materials. Here, EDTA had the most significant affect on both the mechanical properties and the adhesive behavior of dentine. Citric acid did not affect dentine wettability, whereas the indentation properties and the mineralization were reduced. Similar effects were observed when ozone was used. The dentinal tubules were significantly widened in citric acid compared to the ozone group. EDTA causes considerable micromechanical surface alteration of dentine and gutta-percha, but represents the best option in clinical cases where a high adhesiveness of the filling materials is desired. (c) 2015 Elsevier B.V. All rights reserved.
T2  - Applied Surface Science
T1  - Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation
VL  - 355
SP  - 369
EP  - 378
DO  - 10.1016/j.apsusc.2015.07.023
ER  - 

@article{
author = "Antonijević, Đorđe and Milovanović, Petar and Brajkovic, Denis and Ilić, Dragan V. and Hahn, Michael and Amling, Michael and Rakočević, Zlatko Lj. and Đurić, Marija and Busse, Bjoern",
year = "2015",
abstract = "The objective of this study was to determine the effects of various irrigation solutions on root canal dentine and gutta-percha surface properties. In addition, the effects of disinfectant chemicals on the wettability and surface morphological properties of the filling materials were evaluated. Ethylenediaminetetraacetic acid (EDTA), citric acid, and ozone were employed as irrigation solutions for dentine and gutta-percha treatment. Thereafter, the samples microstructure, degree of mineralization, and mechanical properties were assessed by means of quantitative backscattered electron imaging (qBEI) and reference point indentation (RN). A contact angle analyzer was used to measure adhesion on the tested materials. Here, EDTA had the most significant affect on both the mechanical properties and the adhesive behavior of dentine. Citric acid did not affect dentine wettability, whereas the indentation properties and the mineralization were reduced. Similar effects were observed when ozone was used. The dentinal tubules were significantly widened in citric acid compared to the ozone group. EDTA causes considerable micromechanical surface alteration of dentine and gutta-percha, but represents the best option in clinical cases where a high adhesiveness of the filling materials is desired. (c) 2015 Elsevier B.V. All rights reserved.",
journal = "Applied Surface Science",
title = "Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation",
volume = "355",
pages = "369-378",
doi = "10.1016/j.apsusc.2015.07.023"
}

Antonijević, Đ., Milovanović, P., Brajkovic, D., Ilić, D. V., Hahn, M., Amling, M., Rakočević, Z. Lj., Đurić, M.,& Busse, B.. (2015). Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation. in Applied Surface Science, 355, 369-378.
https://doi.org/10.1016/j.apsusc.2015.07.023

Antonijević Đ, Milovanović P, Brajkovic D, Ilić DV, Hahn M, Amling M, Rakočević ZL, Đurić M, Busse B. Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation. in Applied Surface Science. 2015;355:369-378.
doi:10.1016/j.apsusc.2015.07.023 .

Antonijević, Đorđe, Milovanović, Petar, Brajkovic, Denis, Ilić, Dragan V., Hahn, Michael, Amling, Michael, Rakočević, Zlatko Lj., Đurić, Marija, Busse, Bjoern, "Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation" in Applied Surface Science, 355 (2015):369-378,
https://doi.org/10.1016/j.apsusc.2015.07.023 . .

TY  - JOUR
AU  - Milovanović, Petar
AU  - Rakočević, Zlatko Lj.
AU  - Djonic, Danijela
AU  - Živković, Vladimir
AU  - Hahn, Michael
AU  - Nikolic, Slobodan
AU  - Amling, Michael
AU  - Busse, Bjoern
AU  - Đurić, Marija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6001
AB  - To unravel the origins of decreased bone strength in the superolateral femoral neck, we assessed bone structural features across multiple length scales at this cortical fracture initiating region in postmenopausal women with hip fracture and in aged-matched controls. Our combined methodological approach encompassed atomic force microscopy (AFM) characterization of cortical bone nano-structure, assessment of mineral content/distribution via quantitative backscattered electron imaging (qBEI), measurement of bone material properties by reference point indentation, aswell as evaluation of cortical micro-architecture and osteocyte lacunar density. Our findings revealed a wide range of differences between the fracture group and the controls, suggesting a number of detrimental changes at various levels of cortical bone hierarchical organization that may render bone fragile. Namely, mineral crystals at external cortical bone surfaces of the fracture group were larger (65.22 nm +/- 41.21 nm vs. 36.75 nm +/- 18.49 nm, p LT 0.001), and a shift to a higher mineral content and more homogenous mineralization profile as revealed via qBEI were found in the bone matrix of the fracture group. Fracture cases showed nearly 35% higher cortical porosity and showed significantly reduced osteocyte lacunar density compared to controls (226 +/- 27 vs. 247 +/- 32 #/mm2, p= 0.05). Along with increased crystal size, a shift towards higher mineralization and a tendency to increased cortical porosity and reduced osteocyte lacunar number delineate that cortical bone of the superolateral femoral neck bears distinct signs of fragility at various levels of its structural organization. These results contribute to the understanding of hierarchical bone structure changes in age-related fragility. (C) 2014 Elsevier Inc. All rights reserved.
T2  - Experimental Gerontology
T1  - Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls
VL  - 55
SP  - 19
EP  - 28
DO  - 10.1016/j.exger.2014.03.001
ER  - 

@article{
author = "Milovanović, Petar and Rakočević, Zlatko Lj. and Djonic, Danijela and Živković, Vladimir and Hahn, Michael and Nikolic, Slobodan and Amling, Michael and Busse, Bjoern and Đurić, Marija",
year = "2014",
abstract = "To unravel the origins of decreased bone strength in the superolateral femoral neck, we assessed bone structural features across multiple length scales at this cortical fracture initiating region in postmenopausal women with hip fracture and in aged-matched controls. Our combined methodological approach encompassed atomic force microscopy (AFM) characterization of cortical bone nano-structure, assessment of mineral content/distribution via quantitative backscattered electron imaging (qBEI), measurement of bone material properties by reference point indentation, aswell as evaluation of cortical micro-architecture and osteocyte lacunar density. Our findings revealed a wide range of differences between the fracture group and the controls, suggesting a number of detrimental changes at various levels of cortical bone hierarchical organization that may render bone fragile. Namely, mineral crystals at external cortical bone surfaces of the fracture group were larger (65.22 nm +/- 41.21 nm vs. 36.75 nm +/- 18.49 nm, p LT 0.001), and a shift to a higher mineral content and more homogenous mineralization profile as revealed via qBEI were found in the bone matrix of the fracture group. Fracture cases showed nearly 35% higher cortical porosity and showed significantly reduced osteocyte lacunar density compared to controls (226 +/- 27 vs. 247 +/- 32 #/mm2, p= 0.05). Along with increased crystal size, a shift towards higher mineralization and a tendency to increased cortical porosity and reduced osteocyte lacunar number delineate that cortical bone of the superolateral femoral neck bears distinct signs of fragility at various levels of its structural organization. These results contribute to the understanding of hierarchical bone structure changes in age-related fragility. (C) 2014 Elsevier Inc. All rights reserved.",
journal = "Experimental Gerontology",
title = "Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls",
volume = "55",
pages = "19-28",
doi = "10.1016/j.exger.2014.03.001"
}

Milovanović, P., Rakočević, Z. Lj., Djonic, D., Živković, V., Hahn, M., Nikolic, S., Amling, M., Busse, B.,& Đurić, M.. (2014). Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls. in Experimental Gerontology, 55, 19-28.
https://doi.org/10.1016/j.exger.2014.03.001

Milovanović P, Rakočević ZL, Djonic D, Živković V, Hahn M, Nikolic S, Amling M, Busse B, Đurić M. Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls. in Experimental Gerontology. 2014;55:19-28.
doi:10.1016/j.exger.2014.03.001 .

Milovanović, Petar, Rakočević, Zlatko Lj., Djonic, Danijela, Živković, Vladimir, Hahn, Michael, Nikolic, Slobodan, Amling, Michael, Busse, Bjoern, Đurić, Marija, "Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls" in Experimental Gerontology, 55 (2014):19-28,
https://doi.org/10.1016/j.exger.2014.03.001 . .