Spasic, MB

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orcid::0000-0001-9046-0139
  • Spasic, MB (5)
  • Spasic, Mihajlo B. (1)
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Author's Bibliography

Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction

Slavic, Marija; Đorđević, Aleksandar N.; Radojicic, Ratko; Milovanović, Slobodan; Oreščanin-Dušić, Zorana S.; Rakočević, Zlatko Lj.; Spasic, Mihajlo B.; Blagojević, Duško P.

(2013)

TY  - JOUR
AU  - Slavic, Marija
AU  - Đorđević, Aleksandar N.
AU  - Radojicic, Ratko
AU  - Milovanović, Slobodan
AU  - Oreščanin-Dušić, Zorana S.
AU  - Rakočević, Zlatko Lj.
AU  - Spasic, Mihajlo B.
AU  - Blagojević, Duško P.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5496
AB  - Dimethyl sulfoxide (DMSO) is a widely used solvent and cryoprotectant that can cause impaired blood flow, reduction in intracranial pressure, tissue edema, inflammatory reactions, inhibition of vascular smooth muscle cell migration and proliferation, processes which can lead to atherosclerosis of the coronary, peripheral and cerebral circulation. Although the adverse effects are rare when DMSO is administered in clinically established concentrations, there is no safe antagonist for an overdose. In this work, we treated isolated spontaneous and calcium-induced contractile active rat uteri (Wistar, virgo intacta), with DMSO and fullerenol C-60(OH)(24) nanoparticle (FNP) in DMSO. FNP is a water-soluble derivative of fullerene C-60. Its size is a 1.1 nm in diameter and is a very promising candidate for a drug carrier in nanomedicine. FNP also displays free radical scavenging activity. DMSO decreased both spontaneous and calcium-induced contractions. In contrast, FNP only decreased spontaneous contraction. FNP decreased copper-zinc superoxide dismutase activity and prevented the DMSO-induced increase in glutathione reductase activity. Atomic force microscopy detected that FNP aggregated with calcium ions. Our results indicate that FNP has properties that make it a good candidate to be a modulator of DMSO activity which could minimize side effects of the latter.
T2  - Journal of Nanoparticle Research
T1  - Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction
VL  - 15
IS  - 5
DO  - 10.1007/s11051-013-1650-1
ER  - 
@article{
author = "Slavic, Marija and Đorđević, Aleksandar N. and Radojicic, Ratko and Milovanović, Slobodan and Oreščanin-Dušić, Zorana S. and Rakočević, Zlatko Lj. and Spasic, Mihajlo B. and Blagojević, Duško P.",
year = "2013",
abstract = "Dimethyl sulfoxide (DMSO) is a widely used solvent and cryoprotectant that can cause impaired blood flow, reduction in intracranial pressure, tissue edema, inflammatory reactions, inhibition of vascular smooth muscle cell migration and proliferation, processes which can lead to atherosclerosis of the coronary, peripheral and cerebral circulation. Although the adverse effects are rare when DMSO is administered in clinically established concentrations, there is no safe antagonist for an overdose. In this work, we treated isolated spontaneous and calcium-induced contractile active rat uteri (Wistar, virgo intacta), with DMSO and fullerenol C-60(OH)(24) nanoparticle (FNP) in DMSO. FNP is a water-soluble derivative of fullerene C-60. Its size is a 1.1 nm in diameter and is a very promising candidate for a drug carrier in nanomedicine. FNP also displays free radical scavenging activity. DMSO decreased both spontaneous and calcium-induced contractions. In contrast, FNP only decreased spontaneous contraction. FNP decreased copper-zinc superoxide dismutase activity and prevented the DMSO-induced increase in glutathione reductase activity. Atomic force microscopy detected that FNP aggregated with calcium ions. Our results indicate that FNP has properties that make it a good candidate to be a modulator of DMSO activity which could minimize side effects of the latter.",
journal = "Journal of Nanoparticle Research",
title = "Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction",
volume = "15",
number = "5",
doi = "10.1007/s11051-013-1650-1"
}
Slavic, M., Đorđević, A. N., Radojicic, R., Milovanović, S., Oreščanin-Dušić, Z. S., Rakočević, Z. Lj., Spasic, M. B.,& Blagojević, D. P.. (2013). Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction. in Journal of Nanoparticle Research, 15(5).
https://doi.org/10.1007/s11051-013-1650-1
Slavic M, Đorđević AN, Radojicic R, Milovanović S, Oreščanin-Dušić ZS, Rakočević ZL, Spasic MB, Blagojević DP. Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction. in Journal of Nanoparticle Research. 2013;15(5).
doi:10.1007/s11051-013-1650-1 .
Slavic, Marija, Đorđević, Aleksandar N., Radojicic, Ratko, Milovanović, Slobodan, Oreščanin-Dušić, Zorana S., Rakočević, Zlatko Lj., Spasic, Mihajlo B., Blagojević, Duško P., "Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction" in Journal of Nanoparticle Research, 15, no. 5 (2013),
https://doi.org/10.1007/s11051-013-1650-1 . .
15
12
15

Systemic NF-kappa B activation in blood cells of breast cancer patients

Adžić, Miroslav; Nićiforović, Ana; Vučić, Vesna; Nešković-Konstantinović, Zora; Spasić, Snežana D.; Jones, DR; Radoičić, Marija B.; Spasic, MB

(2006)

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Vučić, Vesna
AU  - Nešković-Konstantinović, Zora
AU  - Spasić, Snežana D.
AU  - Jones, DR
AU  - Radoičić, Marija B.
AU  - Spasic, MB
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3008
AB  - There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappa B and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappa B and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.
T2  - Redox Report
T1  - Systemic NF-kappa B activation in blood cells of breast cancer patients
VL  - 11
IS  - 1
SP  - 38
EP  - 44
DO  - 10.1179/135100006X101002
ER  - 
@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Vučić, Vesna and Nešković-Konstantinović, Zora and Spasić, Snežana D. and Jones, DR and Radoičić, Marija B. and Spasic, MB",
year = "2006",
abstract = "There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappa B and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappa B and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.",
journal = "Redox Report",
title = "Systemic NF-kappa B activation in blood cells of breast cancer patients",
volume = "11",
number = "1",
pages = "38-44",
doi = "10.1179/135100006X101002"
}
Adžić, M., Nićiforović, A., Vučić, V., Nešković-Konstantinović, Z., Spasić, S. D., Jones, D., Radoičić, M. B.,& Spasic, M.. (2006). Systemic NF-kappa B activation in blood cells of breast cancer patients. in Redox Report, 11(1), 38-44.
https://doi.org/10.1179/135100006X101002
Adžić M, Nićiforović A, Vučić V, Nešković-Konstantinović Z, Spasić SD, Jones D, Radoičić MB, Spasic M. Systemic NF-kappa B activation in blood cells of breast cancer patients. in Redox Report. 2006;11(1):38-44.
doi:10.1179/135100006X101002 .
Adžić, Miroslav, Nićiforović, Ana, Vučić, Vesna, Nešković-Konstantinović, Zora, Spasić, Snežana D., Jones, DR, Radoičić, Marija B., Spasic, MB, "Systemic NF-kappa B activation in blood cells of breast cancer patients" in Redox Report, 11, no. 1 (2006):38-44,
https://doi.org/10.1179/135100006X101002 . .
10
13

The effect of ovarian hormones on antioxidant enzyme activities in the brain of male rats

Pajović, Snežana B.; Saicic, ZS; Spasic, MB; Petrović, VM

(2003)

TY  - JOUR
AU  - Pajović, Snežana B.
AU  - Saicic, ZS
AU  - Spasic, MB
AU  - Petrović, VM
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2628
AB  - The brain is widely responsive to gonadal hormones. The functional significance of ovarian hormones in the brain is evident from biochemical studies indicating that estradiol or progesterone treatment of testectomized rats produces changes of antioxidant enzyme activities. The effect of estradiol benzoate (EB) and progesterone (P) in the control of antioxidant (AO) enzyme activities was studied in the brain of adult male Wistar rats. The activities of catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) and glutathione reductase (GR) were measured in appropriate subcellular fractions, prepared from brains of animals belonging to various experimental groups. These groups were designed with the intention to follow changes in enzyme activities 2 h or 24 h after systemic administration of 5 mug EB or 2 mg P to testectomized (TX) animals. The obtained results show that both EB and P increase CAT activity, whereas EB decreases GSH-Px, GST and GR activities. These findings clearly show the modulatory role of EB and P in the control of enzymes responsible for the protection of rat nerve cells against oxidative damage caused by free oxygen radicals.
T2  - Physiological Research
T1  - The effect of ovarian hormones on antioxidant enzyme activities in the brain of male rats
VL  - 52
IS  - 2
SP  - 189
EP  - 194
ER  - 
@article{
author = "Pajović, Snežana B. and Saicic, ZS and Spasic, MB and Petrović, VM",
year = "2003",
abstract = "The brain is widely responsive to gonadal hormones. The functional significance of ovarian hormones in the brain is evident from biochemical studies indicating that estradiol or progesterone treatment of testectomized rats produces changes of antioxidant enzyme activities. The effect of estradiol benzoate (EB) and progesterone (P) in the control of antioxidant (AO) enzyme activities was studied in the brain of adult male Wistar rats. The activities of catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) and glutathione reductase (GR) were measured in appropriate subcellular fractions, prepared from brains of animals belonging to various experimental groups. These groups were designed with the intention to follow changes in enzyme activities 2 h or 24 h after systemic administration of 5 mug EB or 2 mg P to testectomized (TX) animals. The obtained results show that both EB and P increase CAT activity, whereas EB decreases GSH-Px, GST and GR activities. These findings clearly show the modulatory role of EB and P in the control of enzymes responsible for the protection of rat nerve cells against oxidative damage caused by free oxygen radicals.",
journal = "Physiological Research",
title = "The effect of ovarian hormones on antioxidant enzyme activities in the brain of male rats",
volume = "52",
number = "2",
pages = "189-194"
}
Pajović, S. B., Saicic, Z., Spasic, M.,& Petrović, V.. (2003). The effect of ovarian hormones on antioxidant enzyme activities in the brain of male rats. in Physiological Research, 52(2), 189-194.
Pajović SB, Saicic Z, Spasic M, Petrović V. The effect of ovarian hormones on antioxidant enzyme activities in the brain of male rats. in Physiological Research. 2003;52(2):189-194..
Pajović, Snežana B., Saicic, ZS, Spasic, MB, Petrović, VM, "The effect of ovarian hormones on antioxidant enzyme activities in the brain of male rats" in Physiological Research, 52, no. 2 (2003):189-194.
34

Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats

Pajović, Snežana B.; Saicic, ZS; Spasic, MB; Petrović, VM; Martinović, Jelena

(1999)

TY  - JOUR
AU  - Pajović, Snežana B.
AU  - Saicic, ZS
AU  - Spasic, MB
AU  - Petrović, VM
AU  - Martinović, Jelena
PY  - 1999
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2251
AB  - The activities of glutathione dependent antioxidant enzymes were measured in subcellular fractions of whole brain homogenates prepared from ovariectomized (OVX) female rats, untreated or treated 2 h or 24 h prior to sacrifice with a single dose of 2 mg progesterone (P) or 5 mu g estradiol benzoate (EB). Glutathione peroxidase (GSH-Px) activity was not changed following systemic administration of EB, but P increased GSH-Px in the brain of OVX rats 24 h after the treatment. The activity of glutathione reductase (GR) was suppressed by EB short time, only 2 h following treatment, whereas P increased the enzyme activity 24 h after treatment. On the other hand, the activities of catalase (CAT) and glutathione-S-transferase (GST) were not changed following systemic administration of EB or P. The present work was carried out to study the involvement of ovarian steroids, especially P, in the control of GSH-Px and GR activities, and our results suggest that oxidative stress in the brain of female rats may be modulated by the level of progesterone.
T2  - General Physiology and Biophysics
T1  - Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats
VL  - 18
IS  - 1
SP  - 35
EP  - 44
ER  - 
@article{
author = "Pajović, Snežana B. and Saicic, ZS and Spasic, MB and Petrović, VM and Martinović, Jelena",
year = "1999",
abstract = "The activities of glutathione dependent antioxidant enzymes were measured in subcellular fractions of whole brain homogenates prepared from ovariectomized (OVX) female rats, untreated or treated 2 h or 24 h prior to sacrifice with a single dose of 2 mg progesterone (P) or 5 mu g estradiol benzoate (EB). Glutathione peroxidase (GSH-Px) activity was not changed following systemic administration of EB, but P increased GSH-Px in the brain of OVX rats 24 h after the treatment. The activity of glutathione reductase (GR) was suppressed by EB short time, only 2 h following treatment, whereas P increased the enzyme activity 24 h after treatment. On the other hand, the activities of catalase (CAT) and glutathione-S-transferase (GST) were not changed following systemic administration of EB or P. The present work was carried out to study the involvement of ovarian steroids, especially P, in the control of GSH-Px and GR activities, and our results suggest that oxidative stress in the brain of female rats may be modulated by the level of progesterone.",
journal = "General Physiology and Biophysics",
title = "Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats",
volume = "18",
number = "1",
pages = "35-44"
}
Pajović, S. B., Saicic, Z., Spasic, M., Petrović, V.,& Martinović, J.. (1999). Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats. in General Physiology and Biophysics, 18(1), 35-44.
Pajović SB, Saicic Z, Spasic M, Petrović V, Martinović J. Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats. in General Physiology and Biophysics. 1999;18(1):35-44..
Pajović, Snežana B., Saicic, ZS, Spasic, MB, Petrović, VM, Martinović, Jelena, "Effects of progesterone and estradiol benzoate on glutathione dependent antioxidant enzyme activities in the brain of female rats" in General Physiology and Biophysics, 18, no. 1 (1999):35-44.
29

Glutathione-dependent antioxidant enzyme activities and glutathione content in the rat brain at different stages of oestrous cycle

Saicic, ZS; Pajović, Snežana B.; Korac, B; Spasic, MB; Martinović, Jelena; Petrović, VM

(1998)

TY  - JOUR
AU  - Saicic, ZS
AU  - Pajović, Snežana B.
AU  - Korac, B
AU  - Spasic, MB
AU  - Martinović, Jelena
AU  - Petrović, VM
PY  - 1998
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2120
AB  - Enzymatic activities of glutathione peroxidase, glutathione-S-transferase, glutathione reductase and catalase, as well as the glutathione content were measured in the brain tissue of regularly cycling rats at dioestrus, proestrus and estrus. The activity of glutathione peroxidase was found to be suppressed at proestrus, whereas that of catalase was increased at dioestrus. Glutathione transferase and glutathione reductase activities, as well as the glutathione content appeared to be stable during the oestrous cycle. These results suggest that, in the female rat, glutathione peroxidase and catalase activities in the brain tissue are influenced by the ovarian hormone status.
T2  - Physiological Research
T1  - Glutathione-dependent antioxidant enzyme activities and glutathione content in the rat brain at different stages of oestrous cycle
VL  - 47
IS  - 1
SP  - 61
EP  - 66
ER  - 
@article{
author = "Saicic, ZS and Pajović, Snežana B. and Korac, B and Spasic, MB and Martinović, Jelena and Petrović, VM",
year = "1998",
abstract = "Enzymatic activities of glutathione peroxidase, glutathione-S-transferase, glutathione reductase and catalase, as well as the glutathione content were measured in the brain tissue of regularly cycling rats at dioestrus, proestrus and estrus. The activity of glutathione peroxidase was found to be suppressed at proestrus, whereas that of catalase was increased at dioestrus. Glutathione transferase and glutathione reductase activities, as well as the glutathione content appeared to be stable during the oestrous cycle. These results suggest that, in the female rat, glutathione peroxidase and catalase activities in the brain tissue are influenced by the ovarian hormone status.",
journal = "Physiological Research",
title = "Glutathione-dependent antioxidant enzyme activities and glutathione content in the rat brain at different stages of oestrous cycle",
volume = "47",
number = "1",
pages = "61-66"
}
Saicic, Z., Pajović, S. B., Korac, B., Spasic, M., Martinović, J.,& Petrović, V.. (1998). Glutathione-dependent antioxidant enzyme activities and glutathione content in the rat brain at different stages of oestrous cycle. in Physiological Research, 47(1), 61-66.
Saicic Z, Pajović SB, Korac B, Spasic M, Martinović J, Petrović V. Glutathione-dependent antioxidant enzyme activities and glutathione content in the rat brain at different stages of oestrous cycle. in Physiological Research. 1998;47(1):61-66..
Saicic, ZS, Pajović, Snežana B., Korac, B, Spasic, MB, Martinović, Jelena, Petrović, VM, "Glutathione-dependent antioxidant enzyme activities and glutathione content in the rat brain at different stages of oestrous cycle" in Physiological Research, 47, no. 1 (1998):61-66.
6

Effects of progesterone and estradiol benzoate on superoxide dismutase activity in the brain of male rats

Pajović, Snežana B.; Saicic, ZS; Spasic, MB; Petrović, VM; Martinović, Jelena

(1996)

TY  - JOUR
AU  - Pajović, Snežana B.
AU  - Saicic, ZS
AU  - Spasic, MB
AU  - Petrović, VM
AU  - Martinović, Jelena
PY  - 1996
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1969
AB  - The activities of mitochondrial, manganese-containing superoxide dismutase (MnSOD) and cytoplasmic, copper-zinc-containing superoxide dismutase (CuZnSOD) were measured in subcellular fractions of whole brain homogenates prepared from intact and gonadectomized (GDX) male rats, untreated or treated subcutaneously (sc) with a single dose of 2 mg progesterone (P) and/or 5 mu g estradiol benzoate (EB). Neither MnSOD nor CuZnSOD was affected by the removal of the testes. Similarly, CuZnSOD activity was steady following systemic administration of P and/or EB to intact and GDX animals 2 h or 24 h prior to sacrifice. On the other hand, both P and EB suppressed MnSOD in the brain of either intact or GDX rats. These results suggest involvement of P and EB in the control of MnSOD activity in the brain of male rats.
T2  - Experientia
T1  - Effects of progesterone and estradiol benzoate on superoxide dismutase activity in the brain of male rats
VL  - 52
IS  - 3
SP  - 221
EP  - 224
ER  - 
@article{
author = "Pajović, Snežana B. and Saicic, ZS and Spasic, MB and Petrović, VM and Martinović, Jelena",
year = "1996",
abstract = "The activities of mitochondrial, manganese-containing superoxide dismutase (MnSOD) and cytoplasmic, copper-zinc-containing superoxide dismutase (CuZnSOD) were measured in subcellular fractions of whole brain homogenates prepared from intact and gonadectomized (GDX) male rats, untreated or treated subcutaneously (sc) with a single dose of 2 mg progesterone (P) and/or 5 mu g estradiol benzoate (EB). Neither MnSOD nor CuZnSOD was affected by the removal of the testes. Similarly, CuZnSOD activity was steady following systemic administration of P and/or EB to intact and GDX animals 2 h or 24 h prior to sacrifice. On the other hand, both P and EB suppressed MnSOD in the brain of either intact or GDX rats. These results suggest involvement of P and EB in the control of MnSOD activity in the brain of male rats.",
journal = "Experientia",
title = "Effects of progesterone and estradiol benzoate on superoxide dismutase activity in the brain of male rats",
volume = "52",
number = "3",
pages = "221-224"
}
Pajović, S. B., Saicic, Z., Spasic, M., Petrović, V.,& Martinović, J.. (1996). Effects of progesterone and estradiol benzoate on superoxide dismutase activity in the brain of male rats. in Experientia, 52(3), 221-224.
Pajović SB, Saicic Z, Spasic M, Petrović V, Martinović J. Effects of progesterone and estradiol benzoate on superoxide dismutase activity in the brain of male rats. in Experientia. 1996;52(3):221-224..
Pajović, Snežana B., Saicic, ZS, Spasic, MB, Petrović, VM, Martinović, Jelena, "Effects of progesterone and estradiol benzoate on superoxide dismutase activity in the brain of male rats" in Experientia, 52, no. 3 (1996):221-224.