TY  - CONF
AU  - Seke, Mariana
AU  - Petrović, Danijela
AU  - Labudović Borović, Milica
AU  - Jović, Danica
AU  - Borišev, Ivana
AU  - Kanački, Zdenko
AU  - Žikić, Dragan
AU  - Đorđević, Aleksandar
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12680
AB  - Introduction: Doxorubicin is the most prominent chemotherapeutic, but its clinical use is limited by its severe systemic toxicity. An iron overload aggravates anthracycline toxicity. Fullerenol in aqueous solutions is in the form of polyanionic nanoparticles, serving as a good carrier of positively charged ions, such as Fe2+. Fullerenol’s antioxidant activity has already been proved in different biological systems. The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite on the rat liver as a pretreatment to doxorubicin application. Methods: After the 24h-treatment, adult male Wistar rats were sacrificed and livers were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, gene expression of enzymes involved in antioxidant defense was measured. Results: Ultrastructural analysis revealed that liver tissue was mainly preserved after the nanocomposite was applied prior to doxorubicin. However, the hepatocytes of animals treated with doxorubicin, presented significantly damaged morphology. Apoptosis of hepatocytes and endothelial cells, mitochondria of irregular size and with disruption of cristae, diffuse injury of capillaries were observed. RT-PCR results have shown that treatment with doxorubicin alone significantly increase the mRNA levels of catalase (p=0.008) and superoxide-dismutase (p=0.000003), while the pretreatment with the nanocomposite prior the doxirubicine treatment, dramaticly downregulated the mRNA levels of catalase (p=0.0004) and superoxide-dismutase (p=0.0001). Conclusion: Our results suggest that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin, demonstrated protection to the liver tissue and induced less damage to the hepatocytes in comparison to doxorubicin alone.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity
SP  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12680
ER  - 

@conference{
author = "Seke, Mariana and Petrović, Danijela and Labudović Borović, Milica and Jović, Danica and Borišev, Ivana and Kanački, Zdenko and Žikić, Dragan and Đorđević, Aleksandar",
year = "2017",
abstract = "Introduction: Doxorubicin is the most prominent chemotherapeutic, but its clinical use is limited by its severe systemic toxicity. An iron overload aggravates anthracycline toxicity. Fullerenol in aqueous solutions is in the form of polyanionic nanoparticles, serving as a good carrier of positively charged ions, such as Fe2+. Fullerenol’s antioxidant activity has already been proved in different biological systems. The aim of our study was to investigate the effects of the fullerenol/iron nanocomposite on the rat liver as a pretreatment to doxorubicin application. Methods: After the 24h-treatment, adult male Wistar rats were sacrificed and livers were collected for ultrastructural and qRT-PCR analysis. Considering the ability of doxorubicin to induce oxidative stress, and the fullerenol’s capability to mitigate it, gene expression of enzymes involved in antioxidant defense was measured. Results: Ultrastructural analysis revealed that liver tissue was mainly preserved after the nanocomposite was applied prior to doxorubicin. However, the hepatocytes of animals treated with doxorubicin, presented significantly damaged morphology. Apoptosis of hepatocytes and endothelial cells, mitochondria of irregular size and with disruption of cristae, diffuse injury of capillaries were observed. RT-PCR results have shown that treatment with doxorubicin alone significantly increase the mRNA levels of catalase (p=0.008) and superoxide-dismutase (p=0.000003), while the pretreatment with the nanocomposite prior the doxirubicine treatment, dramaticly downregulated the mRNA levels of catalase (p=0.0004) and superoxide-dismutase (p=0.0001). Conclusion: Our results suggest that the fullerenol/iron nanocomposite applied as pretreatment to doxorubicin, demonstrated protection to the liver tissue and induced less damage to the hepatocytes in comparison to doxorubicin alone.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity",
pages = "71-71",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12680"
}

Seke, M., Petrović, D., Labudović Borović, M., Jović, D., Borišev, I., Kanački, Z., Žikić, D.,& Đorđević, A.. (2017). Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 71-71.
https://hdl.handle.net/21.15107/rcub_vinar_12680

Seke M, Petrović D, Labudović Borović M, Jović D, Borišev I, Kanački Z, Žikić D, Đorđević A. Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:71-71.
https://hdl.handle.net/21.15107/rcub_vinar_12680 .

Seke, Mariana, Petrović, Danijela, Labudović Borović, Milica, Jović, Danica, Borišev, Ivana, Kanački, Zdenko, Žikić, Dragan, Đorđević, Aleksandar, "Fullerenol/iron nanocomposite modulates doxorubicin-induced hepatotoxicity" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):71-71,
https://hdl.handle.net/21.15107/rcub_vinar_12680 .