TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Jesthke, Anke
AU  - Čolović, Božana M.
AU  - Milovanović, Petar
AU  - Jevremovic, Danimir
AU  - Kisić, Danilo
AU  - vom Scheidt, Annika
AU  - Hahn, Michael
AU  - Amling, Michael
AU  - Jokanović, Vukoman R.
AU  - Busse, Bjoern
AU  - Đurić, Marija
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/847
AB  - Introduction: Calcium silicate cements (CSCs) with the addition of nanohydroxyapatite and calcium carbonate play a critical role in dental applications. To further improve their properties, particularly radiopacity and biointeractivity, the fluoride-containing radiopacifier ytterbium trifluoride (YbF3) was added to their composition, and biological and mechanical characteristics were evaluated. Methods: YbF3 was added to 3 different CSCs: cement I (CSC + calcium carbonate), cement II (CSC + nanohydroxyapatite), and Portland cement. Material characterization encompassed measurements of pH, calcium, ytterbium, and fluoride ion release; radiopacity; setting time; porosity; microindentation properties; wettability; and Fourier transform infrared spectroscopic, x-ray diffraction, and scanning electron microscopic analyses. Osteoblast- and osteoclast-like cells were grown on the materials surface to evaluate their adherence. Results: The addition of calcium carbonate, nanohydroxyapatite, and 30 wt% of YbF3 improved radiopacity and the setting time of experimental cements. The pH values did not differ among the groups. The greatest ytterbium and fluoride releases occurred in the Portland cement + YbF3 group. Combined x-ray diffraction and Fourier transform infrared spectroscopic analysis showed the presence of calcium hydroxide and calcium silicate hydrates. In addition, the presence of calcium ytterbium fluoride and ytterbium oxide proved that YbF3 reacted with cement compounds. Wettability of cement I + YbF3 was superior to other formulations, but its porosity and microindentation properties were weaker than in the Portland cement + YbF3 mixture. Cement II + YbF3 presented micromechanical indentation and porosity characteristics similar to the Portland-based cement formulation. Osteoclast- and osteoblast-like cells adhered to the cements surfaces without alteration of the cell structural integrity. Conclusions: YbF3-containing CSCs with nanostructured hydroxyapatite and calcium carbonate are well suited for dental application.
T2  - Journal of Endodontics
T1  - Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements
VL  - 41
IS  - 12
SP  - 2050
EP  - 2057
DO  - 10.1016/j.joen.2015.09.008
ER  - 

@article{
author = "Antonijević, Đorđe and Jesthke, Anke and Čolović, Božana M. and Milovanović, Petar and Jevremovic, Danimir and Kisić, Danilo and vom Scheidt, Annika and Hahn, Michael and Amling, Michael and Jokanović, Vukoman R. and Busse, Bjoern and Đurić, Marija",
year = "2015",
abstract = "Introduction: Calcium silicate cements (CSCs) with the addition of nanohydroxyapatite and calcium carbonate play a critical role in dental applications. To further improve their properties, particularly radiopacity and biointeractivity, the fluoride-containing radiopacifier ytterbium trifluoride (YbF3) was added to their composition, and biological and mechanical characteristics were evaluated. Methods: YbF3 was added to 3 different CSCs: cement I (CSC + calcium carbonate), cement II (CSC + nanohydroxyapatite), and Portland cement. Material characterization encompassed measurements of pH, calcium, ytterbium, and fluoride ion release; radiopacity; setting time; porosity; microindentation properties; wettability; and Fourier transform infrared spectroscopic, x-ray diffraction, and scanning electron microscopic analyses. Osteoblast- and osteoclast-like cells were grown on the materials surface to evaluate their adherence. Results: The addition of calcium carbonate, nanohydroxyapatite, and 30 wt% of YbF3 improved radiopacity and the setting time of experimental cements. The pH values did not differ among the groups. The greatest ytterbium and fluoride releases occurred in the Portland cement + YbF3 group. Combined x-ray diffraction and Fourier transform infrared spectroscopic analysis showed the presence of calcium hydroxide and calcium silicate hydrates. In addition, the presence of calcium ytterbium fluoride and ytterbium oxide proved that YbF3 reacted with cement compounds. Wettability of cement I + YbF3 was superior to other formulations, but its porosity and microindentation properties were weaker than in the Portland cement + YbF3 mixture. Cement II + YbF3 presented micromechanical indentation and porosity characteristics similar to the Portland-based cement formulation. Osteoclast- and osteoblast-like cells adhered to the cements surfaces without alteration of the cell structural integrity. Conclusions: YbF3-containing CSCs with nanostructured hydroxyapatite and calcium carbonate are well suited for dental application.",
journal = "Journal of Endodontics",
title = "Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements",
volume = "41",
number = "12",
pages = "2050-2057",
doi = "10.1016/j.joen.2015.09.008"
}

Antonijević, Đ., Jesthke, A., Čolović, B. M., Milovanović, P., Jevremovic, D., Kisić, D., vom Scheidt, A., Hahn, M., Amling, M., Jokanović, V. R., Busse, B.,& Đurić, M.. (2015). Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements. in Journal of Endodontics, 41(12), 2050-2057.
https://doi.org/10.1016/j.joen.2015.09.008

Antonijević Đ, Jesthke A, Čolović BM, Milovanović P, Jevremovic D, Kisić D, vom Scheidt A, Hahn M, Amling M, Jokanović VR, Busse B, Đurić M. Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements. in Journal of Endodontics. 2015;41(12):2050-2057.
doi:10.1016/j.joen.2015.09.008 .

Antonijević, Đorđe, Jesthke, Anke, Čolović, Božana M., Milovanović, Petar, Jevremovic, Danimir, Kisić, Danilo, vom Scheidt, Annika, Hahn, Michael, Amling, Michael, Jokanović, Vukoman R., Busse, Bjoern, Đurić, Marija, "Addition of a Fluoride-containing Radiopacifier Improves Micromechanical and Biological Characteristics of Modified Calcium Silicate Cements" in Journal of Endodontics, 41, no. 12 (2015):2050-2057,
https://doi.org/10.1016/j.joen.2015.09.008 . .

TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Milovanović, Petar
AU  - Brajkovic, Denis
AU  - Ilić, Dragan V.
AU  - Hahn, Michael
AU  - Amling, Michael
AU  - Rakočević, Zlatko Lj.
AU  - Đurić, Marija
AU  - Busse, Bjoern
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/791
AB  - The objective of this study was to determine the effects of various irrigation solutions on root canal dentine and gutta-percha surface properties. In addition, the effects of disinfectant chemicals on the wettability and surface morphological properties of the filling materials were evaluated. Ethylenediaminetetraacetic acid (EDTA), citric acid, and ozone were employed as irrigation solutions for dentine and gutta-percha treatment. Thereafter, the samples microstructure, degree of mineralization, and mechanical properties were assessed by means of quantitative backscattered electron imaging (qBEI) and reference point indentation (RN). A contact angle analyzer was used to measure adhesion on the tested materials. Here, EDTA had the most significant affect on both the mechanical properties and the adhesive behavior of dentine. Citric acid did not affect dentine wettability, whereas the indentation properties and the mineralization were reduced. Similar effects were observed when ozone was used. The dentinal tubules were significantly widened in citric acid compared to the ozone group. EDTA causes considerable micromechanical surface alteration of dentine and gutta-percha, but represents the best option in clinical cases where a high adhesiveness of the filling materials is desired. (c) 2015 Elsevier B.V. All rights reserved.
T2  - Applied Surface Science
T1  - Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation
VL  - 355
SP  - 369
EP  - 378
DO  - 10.1016/j.apsusc.2015.07.023
ER  - 

@article{
author = "Antonijević, Đorđe and Milovanović, Petar and Brajkovic, Denis and Ilić, Dragan V. and Hahn, Michael and Amling, Michael and Rakočević, Zlatko Lj. and Đurić, Marija and Busse, Bjoern",
year = "2015",
abstract = "The objective of this study was to determine the effects of various irrigation solutions on root canal dentine and gutta-percha surface properties. In addition, the effects of disinfectant chemicals on the wettability and surface morphological properties of the filling materials were evaluated. Ethylenediaminetetraacetic acid (EDTA), citric acid, and ozone were employed as irrigation solutions for dentine and gutta-percha treatment. Thereafter, the samples microstructure, degree of mineralization, and mechanical properties were assessed by means of quantitative backscattered electron imaging (qBEI) and reference point indentation (RN). A contact angle analyzer was used to measure adhesion on the tested materials. Here, EDTA had the most significant affect on both the mechanical properties and the adhesive behavior of dentine. Citric acid did not affect dentine wettability, whereas the indentation properties and the mineralization were reduced. Similar effects were observed when ozone was used. The dentinal tubules were significantly widened in citric acid compared to the ozone group. EDTA causes considerable micromechanical surface alteration of dentine and gutta-percha, but represents the best option in clinical cases where a high adhesiveness of the filling materials is desired. (c) 2015 Elsevier B.V. All rights reserved.",
journal = "Applied Surface Science",
title = "Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation",
volume = "355",
pages = "369-378",
doi = "10.1016/j.apsusc.2015.07.023"
}

Antonijević, Đ., Milovanović, P., Brajkovic, D., Ilić, D. V., Hahn, M., Amling, M., Rakočević, Z. Lj., Đurić, M.,& Busse, B.. (2015). Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation. in Applied Surface Science, 355, 369-378.
https://doi.org/10.1016/j.apsusc.2015.07.023

Antonijević Đ, Milovanović P, Brajkovic D, Ilić DV, Hahn M, Amling M, Rakočević ZL, Đurić M, Busse B. Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation. in Applied Surface Science. 2015;355:369-378.
doi:10.1016/j.apsusc.2015.07.023 .

Antonijević, Đorđe, Milovanović, Petar, Brajkovic, Denis, Ilić, Dragan V., Hahn, Michael, Amling, Michael, Rakočević, Zlatko Lj., Đurić, Marija, Busse, Bjoern, "Microstructure and wettability of root canal dentine and root canal filling materials after different chemical irrigation" in Applied Surface Science, 355 (2015):369-378,
https://doi.org/10.1016/j.apsusc.2015.07.023 . .

TY  - JOUR
AU  - Zelic, Ksenija
AU  - Milovanović, Petar
AU  - Rakočević, Zlatko Lj.
AU  - Aškrabić, Sonja
AU  - Potočnik, Jelena
AU  - Popovic, Miroslav
AU  - Đurić, Marija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5962
AB  - Objective. Increased tooth fragility after devitalization is commonly observed but there is no definite mechanistic explanation for such phenomenon. Therefore, it is important to analyze more profoundly structural and compositional properties of this altered form of dentin. The present study investigates the differences between normal and devitalized dentin using advanced techniques. Methods. Atomic force microscopic imaging (AFM), energy dispersive X-ray analysis (EDX) and micro-Raman spectroscopy were performed on 16 dentin specimens, eight vital and eight that underwent root-canal treatment at least two years before extraction and had no infection in root canals before or after devitalization. Results. The mean size of mineral crystals showed by AFM was larger in devitalized than in healthy dentin in the same age category. AFM phase shifts in devitalized cases revealed altered mechanical characteristics and suggested differences in composition of material between devitalized teeth and healthy controls. No significant difference in Ca/P ratio between vital and devitalized teeth was found using EDX. However, micro-Raman analyses showed that in devitalized teeth, apart from hydroxyapatite, dentin contained significant amounts of apatite phases with lower calcium content: octacalcium phosphate, dicalcium phosphate dihydrate and tricalcium phosphate. Significance. Differences between vital and devitalized dentin bring new insights into the basis of devitalized tooth fragility. Larger mineral crystals could account for decreased mechanical strength in devitalized teeth. Moreover, calcium-phosphate phases with lower Ca content have lower material strength, and the presence of these phases in devitalized teeth may explain their increased fragility. (C) 2014 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
T2  - Dental Materials
T1  - Nano-structural and compositional basis of devitalized tooth fragility
VL  - 30
IS  - 5
SP  - 476
EP  - 486
DO  - 10.1016/j.dental.2014.01.014
ER  - 

@article{
author = "Zelic, Ksenija and Milovanović, Petar and Rakočević, Zlatko Lj. and Aškrabić, Sonja and Potočnik, Jelena and Popovic, Miroslav and Đurić, Marija",
year = "2014",
abstract = "Objective. Increased tooth fragility after devitalization is commonly observed but there is no definite mechanistic explanation for such phenomenon. Therefore, it is important to analyze more profoundly structural and compositional properties of this altered form of dentin. The present study investigates the differences between normal and devitalized dentin using advanced techniques. Methods. Atomic force microscopic imaging (AFM), energy dispersive X-ray analysis (EDX) and micro-Raman spectroscopy were performed on 16 dentin specimens, eight vital and eight that underwent root-canal treatment at least two years before extraction and had no infection in root canals before or after devitalization. Results. The mean size of mineral crystals showed by AFM was larger in devitalized than in healthy dentin in the same age category. AFM phase shifts in devitalized cases revealed altered mechanical characteristics and suggested differences in composition of material between devitalized teeth and healthy controls. No significant difference in Ca/P ratio between vital and devitalized teeth was found using EDX. However, micro-Raman analyses showed that in devitalized teeth, apart from hydroxyapatite, dentin contained significant amounts of apatite phases with lower calcium content: octacalcium phosphate, dicalcium phosphate dihydrate and tricalcium phosphate. Significance. Differences between vital and devitalized dentin bring new insights into the basis of devitalized tooth fragility. Larger mineral crystals could account for decreased mechanical strength in devitalized teeth. Moreover, calcium-phosphate phases with lower Ca content have lower material strength, and the presence of these phases in devitalized teeth may explain their increased fragility. (C) 2014 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.",
journal = "Dental Materials",
title = "Nano-structural and compositional basis of devitalized tooth fragility",
volume = "30",
number = "5",
pages = "476-486",
doi = "10.1016/j.dental.2014.01.014"
}

Zelic, K., Milovanović, P., Rakočević, Z. Lj., Aškrabić, S., Potočnik, J., Popovic, M.,& Đurić, M.. (2014). Nano-structural and compositional basis of devitalized tooth fragility. in Dental Materials, 30(5), 476-486.
https://doi.org/10.1016/j.dental.2014.01.014

Zelic K, Milovanović P, Rakočević ZL, Aškrabić S, Potočnik J, Popovic M, Đurić M. Nano-structural and compositional basis of devitalized tooth fragility. in Dental Materials. 2014;30(5):476-486.
doi:10.1016/j.dental.2014.01.014 .

Zelic, Ksenija, Milovanović, Petar, Rakočević, Zlatko Lj., Aškrabić, Sonja, Potočnik, Jelena, Popovic, Miroslav, Đurić, Marija, "Nano-structural and compositional basis of devitalized tooth fragility" in Dental Materials, 30, no. 5 (2014):476-486,
https://doi.org/10.1016/j.dental.2014.01.014 . .

TY  - JOUR
AU  - Brajkovic, Denis
AU  - Antonijević, Đorđe
AU  - Milovanović, Petar
AU  - Kisić, Danilo
AU  - Zelic, Ksenija
AU  - Đurić, Marija
AU  - Rakočević, Zlatko Lj.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6071
AB  - Background: Material surface free energy and surface roughness strongly influence the bacterial adhesion in oral cavity. The aim of this study was to analyze these two parameters in various commercial luting agents used for cementation of implant restorations. Materials and methods: Zinc-based, glass-ionomers, resin modified glass-ionomer and resin-cements were investigated. Contact angle and surface free energy were measured by contact angle analyzer using Image J software program. Materials average roughness and fractal dimension were calculated based on Atomic Force Microscope topography images. Results: Zinc phosphate cements presented significantly higher total surface free energy and significantly lower dispersive component of surface free energy compared to other groups, while resin-cements showed significantly lower polar component than other groups. The surface roughness and fractal dimension values were statistically the highest in the zinc phosphate cements and the lowest for the glass-ionomers cements. Conclusion: Glass-ionomers-cements presented lower values of surface free energy and surface roughness than zinc phosphate and resin cements, indicating that their surfaces are less prone to biofilm adhesion. Practical implications: Within limitations of an in vitro trial, our results indicate that glass-ionomers-cements could be the cements of choice for fixation of cement retained implant restorations due to superior surface properties compared to zinc phosphate and resin cements, which may result in reduced plaque formation and presumably prevent periimplant infection. (C) 2014 Elsevier B.V. All rights reserved.
T2  - Applied Surface Science
T1  - Surface characterization of the cement for retention of implant supported dental prostheses: In vitro evaluation of cement roughness and surface free energy
VL  - 311
SP  - 131
EP  - 138
DO  - 10.1016/j.apsusc.2014.05.027
ER  - 

@article{
author = "Brajkovic, Denis and Antonijević, Đorđe and Milovanović, Petar and Kisić, Danilo and Zelic, Ksenija and Đurić, Marija and Rakočević, Zlatko Lj.",
year = "2014",
abstract = "Background: Material surface free energy and surface roughness strongly influence the bacterial adhesion in oral cavity. The aim of this study was to analyze these two parameters in various commercial luting agents used for cementation of implant restorations. Materials and methods: Zinc-based, glass-ionomers, resin modified glass-ionomer and resin-cements were investigated. Contact angle and surface free energy were measured by contact angle analyzer using Image J software program. Materials average roughness and fractal dimension were calculated based on Atomic Force Microscope topography images. Results: Zinc phosphate cements presented significantly higher total surface free energy and significantly lower dispersive component of surface free energy compared to other groups, while resin-cements showed significantly lower polar component than other groups. The surface roughness and fractal dimension values were statistically the highest in the zinc phosphate cements and the lowest for the glass-ionomers cements. Conclusion: Glass-ionomers-cements presented lower values of surface free energy and surface roughness than zinc phosphate and resin cements, indicating that their surfaces are less prone to biofilm adhesion. Practical implications: Within limitations of an in vitro trial, our results indicate that glass-ionomers-cements could be the cements of choice for fixation of cement retained implant restorations due to superior surface properties compared to zinc phosphate and resin cements, which may result in reduced plaque formation and presumably prevent periimplant infection. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Applied Surface Science",
title = "Surface characterization of the cement for retention of implant supported dental prostheses: In vitro evaluation of cement roughness and surface free energy",
volume = "311",
pages = "131-138",
doi = "10.1016/j.apsusc.2014.05.027"
}

Brajkovic, D., Antonijević, Đ., Milovanović, P., Kisić, D., Zelic, K., Đurić, M.,& Rakočević, Z. Lj.. (2014). Surface characterization of the cement for retention of implant supported dental prostheses: In vitro evaluation of cement roughness and surface free energy. in Applied Surface Science, 311, 131-138.
https://doi.org/10.1016/j.apsusc.2014.05.027

Brajkovic D, Antonijević Đ, Milovanović P, Kisić D, Zelic K, Đurić M, Rakočević ZL. Surface characterization of the cement for retention of implant supported dental prostheses: In vitro evaluation of cement roughness and surface free energy. in Applied Surface Science. 2014;311:131-138.
doi:10.1016/j.apsusc.2014.05.027 .

Brajkovic, Denis, Antonijević, Đorđe, Milovanović, Petar, Kisić, Danilo, Zelic, Ksenija, Đurić, Marija, Rakočević, Zlatko Lj., "Surface characterization of the cement for retention of implant supported dental prostheses: In vitro evaluation of cement roughness and surface free energy" in Applied Surface Science, 311 (2014):131-138,
https://doi.org/10.1016/j.apsusc.2014.05.027 . .

TY  - JOUR
AU  - Milovanović, Petar
AU  - Rakočević, Zlatko Lj.
AU  - Djonic, Danijela
AU  - Živković, Vladimir
AU  - Hahn, Michael
AU  - Nikolic, Slobodan
AU  - Amling, Michael
AU  - Busse, Bjoern
AU  - Đurić, Marija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6001
AB  - To unravel the origins of decreased bone strength in the superolateral femoral neck, we assessed bone structural features across multiple length scales at this cortical fracture initiating region in postmenopausal women with hip fracture and in aged-matched controls. Our combined methodological approach encompassed atomic force microscopy (AFM) characterization of cortical bone nano-structure, assessment of mineral content/distribution via quantitative backscattered electron imaging (qBEI), measurement of bone material properties by reference point indentation, aswell as evaluation of cortical micro-architecture and osteocyte lacunar density. Our findings revealed a wide range of differences between the fracture group and the controls, suggesting a number of detrimental changes at various levels of cortical bone hierarchical organization that may render bone fragile. Namely, mineral crystals at external cortical bone surfaces of the fracture group were larger (65.22 nm +/- 41.21 nm vs. 36.75 nm +/- 18.49 nm, p LT 0.001), and a shift to a higher mineral content and more homogenous mineralization profile as revealed via qBEI were found in the bone matrix of the fracture group. Fracture cases showed nearly 35% higher cortical porosity and showed significantly reduced osteocyte lacunar density compared to controls (226 +/- 27 vs. 247 +/- 32 #/mm2, p= 0.05). Along with increased crystal size, a shift towards higher mineralization and a tendency to increased cortical porosity and reduced osteocyte lacunar number delineate that cortical bone of the superolateral femoral neck bears distinct signs of fragility at various levels of its structural organization. These results contribute to the understanding of hierarchical bone structure changes in age-related fragility. (C) 2014 Elsevier Inc. All rights reserved.
T2  - Experimental Gerontology
T1  - Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls
VL  - 55
SP  - 19
EP  - 28
DO  - 10.1016/j.exger.2014.03.001
ER  - 

@article{
author = "Milovanović, Petar and Rakočević, Zlatko Lj. and Djonic, Danijela and Živković, Vladimir and Hahn, Michael and Nikolic, Slobodan and Amling, Michael and Busse, Bjoern and Đurić, Marija",
year = "2014",
abstract = "To unravel the origins of decreased bone strength in the superolateral femoral neck, we assessed bone structural features across multiple length scales at this cortical fracture initiating region in postmenopausal women with hip fracture and in aged-matched controls. Our combined methodological approach encompassed atomic force microscopy (AFM) characterization of cortical bone nano-structure, assessment of mineral content/distribution via quantitative backscattered electron imaging (qBEI), measurement of bone material properties by reference point indentation, aswell as evaluation of cortical micro-architecture and osteocyte lacunar density. Our findings revealed a wide range of differences between the fracture group and the controls, suggesting a number of detrimental changes at various levels of cortical bone hierarchical organization that may render bone fragile. Namely, mineral crystals at external cortical bone surfaces of the fracture group were larger (65.22 nm +/- 41.21 nm vs. 36.75 nm +/- 18.49 nm, p LT 0.001), and a shift to a higher mineral content and more homogenous mineralization profile as revealed via qBEI were found in the bone matrix of the fracture group. Fracture cases showed nearly 35% higher cortical porosity and showed significantly reduced osteocyte lacunar density compared to controls (226 +/- 27 vs. 247 +/- 32 #/mm2, p= 0.05). Along with increased crystal size, a shift towards higher mineralization and a tendency to increased cortical porosity and reduced osteocyte lacunar number delineate that cortical bone of the superolateral femoral neck bears distinct signs of fragility at various levels of its structural organization. These results contribute to the understanding of hierarchical bone structure changes in age-related fragility. (C) 2014 Elsevier Inc. All rights reserved.",
journal = "Experimental Gerontology",
title = "Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls",
volume = "55",
pages = "19-28",
doi = "10.1016/j.exger.2014.03.001"
}

Milovanović, P., Rakočević, Z. Lj., Djonic, D., Živković, V., Hahn, M., Nikolic, S., Amling, M., Busse, B.,& Đurić, M.. (2014). Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls. in Experimental Gerontology, 55, 19-28.
https://doi.org/10.1016/j.exger.2014.03.001

Milovanović P, Rakočević ZL, Djonic D, Živković V, Hahn M, Nikolic S, Amling M, Busse B, Đurić M. Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls. in Experimental Gerontology. 2014;55:19-28.
doi:10.1016/j.exger.2014.03.001 .

Milovanović, Petar, Rakočević, Zlatko Lj., Djonic, Danijela, Živković, Vladimir, Hahn, Michael, Nikolic, Slobodan, Amling, Michael, Busse, Bjoern, Đurić, Marija, "Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls" in Experimental Gerontology, 55 (2014):19-28,
https://doi.org/10.1016/j.exger.2014.03.001 . .

TY  - CONF
AU  - Zelić Mihajlović, Ksenija
AU  - Milovanović, Petar
AU  - Rakočević, Zlatko Lj.
AU  - Aškrabić, Sonja
AU  - Potočnik, Jelena
AU  - Popović, Miroslav
AU  - Đurić, Marija
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10778
AB  - Tooth fracture is considered as a major problem in dentistry. As it is commonly observed in dental practice, one of the main factors that lead to increased tooth fragility is its devitalization. However, there is no definite mechanistic explanation for such phenomenon. We hypothesize that the possible response to this matter lies in the changes that occur in dentin due to altered microenvironment after endodontic procedure. Therefore, in this study we analyzed the structural and compositional differences between vital and devitalized dentine.  Atomic force microscopic imaging (AFM), and micro-Raman spectroscopy were performed on 16 dentine specimens, eight taken from vital teeth and eight taken from teeth that underwent root-canal treatment at least 2 years before extraction and had no infection in root canals. All teeth were upper premolars.  The mean size of mineral grains, showed by AFM topography images, was larger in devitalized than in healthy dentine in the same age category. AFM phase shifts in devitalized cases revealed altered mechanical characteristics and suggested differences in composition of material between devitalized teeth and healthy controls. Micro-Raman analyses showed that in devitalized teeth, apart from hydroxyapatite, dentine contained significant amounts of apatite phases with lower calcium content: octacalcium phosphate, dicalcium phosphate dihidrate and tricalcium phosphate.  Differences between vital and devitalized dentine bring new insight into basis of devitalized tooth fragility. Larger mineral grains could account for decreased mechanical strength in devitalized teeth. Moreover, calcium-phosphate phases with lower Ca content have lower material strength, and the presence of these phases in devitalized teeth may explain their increased fragility.
C3  - European Calcified Tissue Society Congress 2013 : Bone Abstracts
T1  - Nano-structural and compositional basis of devitalized tooth fragility
VL  - 1
SP  - pp49
DO  - 10.1530/boneabs.1.PP49
ER  - 

@conference{
author = "Zelić Mihajlović, Ksenija and Milovanović, Petar and Rakočević, Zlatko Lj. and Aškrabić, Sonja and Potočnik, Jelena and Popović, Miroslav and Đurić, Marija",
year = "2013",
abstract = "Tooth fracture is considered as a major problem in dentistry. As it is commonly observed in dental practice, one of the main factors that lead to increased tooth fragility is its devitalization. However, there is no definite mechanistic explanation for such phenomenon. We hypothesize that the possible response to this matter lies in the changes that occur in dentin due to altered microenvironment after endodontic procedure. Therefore, in this study we analyzed the structural and compositional differences between vital and devitalized dentine.  Atomic force microscopic imaging (AFM), and micro-Raman spectroscopy were performed on 16 dentine specimens, eight taken from vital teeth and eight taken from teeth that underwent root-canal treatment at least 2 years before extraction and had no infection in root canals. All teeth were upper premolars.  The mean size of mineral grains, showed by AFM topography images, was larger in devitalized than in healthy dentine in the same age category. AFM phase shifts in devitalized cases revealed altered mechanical characteristics and suggested differences in composition of material between devitalized teeth and healthy controls. Micro-Raman analyses showed that in devitalized teeth, apart from hydroxyapatite, dentine contained significant amounts of apatite phases with lower calcium content: octacalcium phosphate, dicalcium phosphate dihidrate and tricalcium phosphate.  Differences between vital and devitalized dentine bring new insight into basis of devitalized tooth fragility. Larger mineral grains could account for decreased mechanical strength in devitalized teeth. Moreover, calcium-phosphate phases with lower Ca content have lower material strength, and the presence of these phases in devitalized teeth may explain their increased fragility.",
journal = "European Calcified Tissue Society Congress 2013 : Bone Abstracts",
title = "Nano-structural and compositional basis of devitalized tooth fragility",
volume = "1",
pages = "pp49",
doi = "10.1530/boneabs.1.PP49"
}

Zelić Mihajlović, K., Milovanović, P., Rakočević, Z. Lj., Aškrabić, S., Potočnik, J., Popović, M.,& Đurić, M.. (2013). Nano-structural and compositional basis of devitalized tooth fragility. in European Calcified Tissue Society Congress 2013 : Bone Abstracts, 1, pp49.
https://doi.org/10.1530/boneabs.1.PP49

Zelić Mihajlović K, Milovanović P, Rakočević ZL, Aškrabić S, Potočnik J, Popović M, Đurić M. Nano-structural and compositional basis of devitalized tooth fragility. in European Calcified Tissue Society Congress 2013 : Bone Abstracts. 2013;1:pp49.
doi:10.1530/boneabs.1.PP49 .

Zelić Mihajlović, Ksenija, Milovanović, Petar, Rakočević, Zlatko Lj., Aškrabić, Sonja, Potočnik, Jelena, Popović, Miroslav, Đurić, Marija, "Nano-structural and compositional basis of devitalized tooth fragility" in European Calcified Tissue Society Congress 2013 : Bone Abstracts, 1 (2013):pp49,
https://doi.org/10.1530/boneabs.1.PP49 . .

TY  - JOUR
AU  - Milovanović, Petar
AU  - Đurić, Marija
AU  - Nešković, Olivera M.
AU  - Djonic, Danijela
AU  - Potočnik, Jelena
AU  - Nikolic, Slobodan
AU  - Stoiljković, Milovan
AU  - Živković, Vladimir
AU  - Rakočević, Zlatko Lj.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5687
AB  - On the basis of the suggestion that bone nanostructure bears tissue age information and may reflect surface deposition/modification processes, we performed nanoscale characterization of the external cortical bone surface at the femoral neck in women using atomic force microscopy (AFM). The specific aims were to assess age-related differences in bone nanostructure and explore the existence of nanostructural traces of potential bone apposition at this surface. Our findings revealed that the external cortical surface represents a continuous phase composed of densely packed mineral grains. Although the grains varied in size and shape, there was a domination of small grains indicative of freshly deposited bone (mean grain size: young, 35 nm; old, 37 nm; p. 0.05). Advanced quantitative analysis of surface morphological patterns revealed comparable roughness and complexity of the surface, suggesting a similar rate of mineral particle deposition at the surface in both groups. Calcium/phosphorus ratio, a measure of bone tissue age, was within the same range in both groups. In summary, our AFM analyses showed consistent nanostructural and compositional bone features, suggesting existence of new bone at the periosteal bone surface in both young and elderly women. Considering observed age-related increase in the neck diameter, AFM findings may support the theory of continuous bone apposition at the periosteal surface.
T2  - Microscopy and Microanalysis
T1  - Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging
VL  - 19
IS  - 5
SP  - 1341
EP  - 1349
DO  - 10.1017/S1431927613001761
ER  - 

@article{
author = "Milovanović, Petar and Đurić, Marija and Nešković, Olivera M. and Djonic, Danijela and Potočnik, Jelena and Nikolic, Slobodan and Stoiljković, Milovan and Živković, Vladimir and Rakočević, Zlatko Lj.",
year = "2013",
abstract = "On the basis of the suggestion that bone nanostructure bears tissue age information and may reflect surface deposition/modification processes, we performed nanoscale characterization of the external cortical bone surface at the femoral neck in women using atomic force microscopy (AFM). The specific aims were to assess age-related differences in bone nanostructure and explore the existence of nanostructural traces of potential bone apposition at this surface. Our findings revealed that the external cortical surface represents a continuous phase composed of densely packed mineral grains. Although the grains varied in size and shape, there was a domination of small grains indicative of freshly deposited bone (mean grain size: young, 35 nm; old, 37 nm; p. 0.05). Advanced quantitative analysis of surface morphological patterns revealed comparable roughness and complexity of the surface, suggesting a similar rate of mineral particle deposition at the surface in both groups. Calcium/phosphorus ratio, a measure of bone tissue age, was within the same range in both groups. In summary, our AFM analyses showed consistent nanostructural and compositional bone features, suggesting existence of new bone at the periosteal bone surface in both young and elderly women. Considering observed age-related increase in the neck diameter, AFM findings may support the theory of continuous bone apposition at the periosteal surface.",
journal = "Microscopy and Microanalysis",
title = "Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging",
volume = "19",
number = "5",
pages = "1341-1349",
doi = "10.1017/S1431927613001761"
}

Milovanović, P., Đurić, M., Nešković, O. M., Djonic, D., Potočnik, J., Nikolic, S., Stoiljković, M., Živković, V.,& Rakočević, Z. Lj.. (2013). Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging. in Microscopy and Microanalysis, 19(5), 1341-1349.
https://doi.org/10.1017/S1431927613001761

Milovanović P, Đurić M, Nešković OM, Djonic D, Potočnik J, Nikolic S, Stoiljković M, Živković V, Rakočević ZL. Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging. in Microscopy and Microanalysis. 2013;19(5):1341-1349.
doi:10.1017/S1431927613001761 .

Milovanović, Petar, Đurić, Marija, Nešković, Olivera M., Djonic, Danijela, Potočnik, Jelena, Nikolic, Slobodan, Stoiljković, Milovan, Živković, Vladimir, Rakočević, Zlatko Lj., "Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging" in Microscopy and Microanalysis, 19, no. 5 (2013):1341-1349,
https://doi.org/10.1017/S1431927613001761 . .

TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Denis, Brajkovic
AU  - Nenadović, Miloš
AU  - Milovanović, Petar
AU  - Marija, Djuric
AU  - Rakočević, Zlatko Lj.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5637
AB  - Objective: The aim of this in vitro study was to compare the surface roughness parameters of four different types of dental luting agents used for cementation of implant restorations. Method and materials: Five specimens (8 mm high and 1 mm thick) of each cement were made using metal ring steelless molds. Atomic Force Microscope was employed to analyze different surface texture parameters of the materials. Bearing ratio analysis was used to calculate the potential microgap size between the cement and implant material and to calculate the depth of the valleys on the cement surface, while power spectral density (PSD) measurements were performed to measure the percentage of the surface prone to bacterial adhesion. Results: Glass ionomer cement showed significantly lower value of average surface roughness then the other groups of the materials (P LT 0.05) which was in line with the results of Bearing ratio analysis. On the other side, PSD analysis showed that zinc phosphate cement experience the lowest percentage of the surface which promote bacterial colonization. Conclusion: Glas ionomer cements present the surface roughness parameters that are less favorable for bacterial adhesion than that of zinc phosphate, resin-modified glass ionomer and resin cements. Microsc. Res. Tech. 76:924-930, 2013. (c) 2013 Wiley Periodicals, Inc.
T2  - Microscopy Research and Technique
T1  - An in vitro atomic force microscopic study of commercially available dental luting materials
VL  - 76
IS  - 9
SP  - 924
EP  - 930
DO  - 10.1002/jemt.22249
ER  - 

@article{
author = "Antonijević, Đorđe and Denis, Brajkovic and Nenadović, Miloš and Milovanović, Petar and Marija, Djuric and Rakočević, Zlatko Lj.",
year = "2013",
abstract = "Objective: The aim of this in vitro study was to compare the surface roughness parameters of four different types of dental luting agents used for cementation of implant restorations. Method and materials: Five specimens (8 mm high and 1 mm thick) of each cement were made using metal ring steelless molds. Atomic Force Microscope was employed to analyze different surface texture parameters of the materials. Bearing ratio analysis was used to calculate the potential microgap size between the cement and implant material and to calculate the depth of the valleys on the cement surface, while power spectral density (PSD) measurements were performed to measure the percentage of the surface prone to bacterial adhesion. Results: Glass ionomer cement showed significantly lower value of average surface roughness then the other groups of the materials (P LT 0.05) which was in line with the results of Bearing ratio analysis. On the other side, PSD analysis showed that zinc phosphate cement experience the lowest percentage of the surface which promote bacterial colonization. Conclusion: Glas ionomer cements present the surface roughness parameters that are less favorable for bacterial adhesion than that of zinc phosphate, resin-modified glass ionomer and resin cements. Microsc. Res. Tech. 76:924-930, 2013. (c) 2013 Wiley Periodicals, Inc.",
journal = "Microscopy Research and Technique",
title = "An in vitro atomic force microscopic study of commercially available dental luting materials",
volume = "76",
number = "9",
pages = "924-930",
doi = "10.1002/jemt.22249"
}

Antonijević, Đ., Denis, B., Nenadović, M., Milovanović, P., Marija, D.,& Rakočević, Z. Lj.. (2013). An in vitro atomic force microscopic study of commercially available dental luting materials. in Microscopy Research and Technique, 76(9), 924-930.
https://doi.org/10.1002/jemt.22249

Antonijević Đ, Denis B, Nenadović M, Milovanović P, Marija D, Rakočević ZL. An in vitro atomic force microscopic study of commercially available dental luting materials. in Microscopy Research and Technique. 2013;76(9):924-930.
doi:10.1002/jemt.22249 .

Antonijević, Đorđe, Denis, Brajkovic, Nenadović, Miloš, Milovanović, Petar, Marija, Djuric, Rakočević, Zlatko Lj., "An in vitro atomic force microscopic study of commercially available dental luting materials" in Microscopy Research and Technique, 76, no. 9 (2013):924-930,
https://doi.org/10.1002/jemt.22249 . .

TY  - JOUR
AU  - Milovanović, Petar
AU  - Potočnik, Jelena
AU  - Djonic, Danijela
AU  - Nikolic, Slobodan
AU  - Živković, Vladimir
AU  - Đurić, Marija
AU  - Rakočević, Zlatko Lj.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4716
AB  - Despite general belief that the mechanical properties of bone material contribute to whole bone strength, it is still obscure what the age effects are on mechanical behavior of the bone material, particularly in the case of the femoral neck trabeculae. In this study, atomic force microscopy was used for imaging and measuring the size of mineral grains, as well as nano-scale mechanical characterization (nanoindentation) of the bone mineralized matrix of trabeculae, with the aim to explore the age effects on bone elasticity and give new insight into age-related bone fragility. The bone samples in this study comprised trabecular bone specimens of the femoral neck region, collected from eight skeletal healthy women (five young adults: 27-38 yrs., three elderly: 83-94 yrs.) at autopsy. Bone trabeculae in the elderly displayed a higher modulus and nanohardness, signifying a decreased amount of energy that can be accommodated by the bone tissue during loading. Regression analysis revealed that nearly 65% of variability in the bone matrix elastic modulus can be statistically explained by the changes in size of the matrix mineral grains. This study revealed that the bone trabeculae of elderly women express less elastic behavior at the material level, which makes them more vulnerable to unusual impact loads originating from a fall. The observed age-related structural and mechanical alteration at the bone material level adds new evidence for understanding why hip fractures are more frequent in elderly women. (C) 2011 Elsevier Inc. All rights reserved.
T2  - Experimental Gerontology
T1  - Age-related deterioration in trabecular bone mechanical properties at material level: Nanoindentation study of the femoral neck in women by using AFM
VL  - 47
IS  - 2
SP  - 154
EP  - 159
DO  - 10.1016/j.exger.2011.11.011
ER  - 

@article{
author = "Milovanović, Petar and Potočnik, Jelena and Djonic, Danijela and Nikolic, Slobodan and Živković, Vladimir and Đurić, Marija and Rakočević, Zlatko Lj.",
year = "2012",
abstract = "Despite general belief that the mechanical properties of bone material contribute to whole bone strength, it is still obscure what the age effects are on mechanical behavior of the bone material, particularly in the case of the femoral neck trabeculae. In this study, atomic force microscopy was used for imaging and measuring the size of mineral grains, as well as nano-scale mechanical characterization (nanoindentation) of the bone mineralized matrix of trabeculae, with the aim to explore the age effects on bone elasticity and give new insight into age-related bone fragility. The bone samples in this study comprised trabecular bone specimens of the femoral neck region, collected from eight skeletal healthy women (five young adults: 27-38 yrs., three elderly: 83-94 yrs.) at autopsy. Bone trabeculae in the elderly displayed a higher modulus and nanohardness, signifying a decreased amount of energy that can be accommodated by the bone tissue during loading. Regression analysis revealed that nearly 65% of variability in the bone matrix elastic modulus can be statistically explained by the changes in size of the matrix mineral grains. This study revealed that the bone trabeculae of elderly women express less elastic behavior at the material level, which makes them more vulnerable to unusual impact loads originating from a fall. The observed age-related structural and mechanical alteration at the bone material level adds new evidence for understanding why hip fractures are more frequent in elderly women. (C) 2011 Elsevier Inc. All rights reserved.",
journal = "Experimental Gerontology",
title = "Age-related deterioration in trabecular bone mechanical properties at material level: Nanoindentation study of the femoral neck in women by using AFM",
volume = "47",
number = "2",
pages = "154-159",
doi = "10.1016/j.exger.2011.11.011"
}

Milovanović, P., Potočnik, J., Djonic, D., Nikolic, S., Živković, V., Đurić, M.,& Rakočević, Z. Lj.. (2012). Age-related deterioration in trabecular bone mechanical properties at material level: Nanoindentation study of the femoral neck in women by using AFM. in Experimental Gerontology, 47(2), 154-159.
https://doi.org/10.1016/j.exger.2011.11.011

Milovanović P, Potočnik J, Djonic D, Nikolic S, Živković V, Đurić M, Rakočević ZL. Age-related deterioration in trabecular bone mechanical properties at material level: Nanoindentation study of the femoral neck in women by using AFM. in Experimental Gerontology. 2012;47(2):154-159.
doi:10.1016/j.exger.2011.11.011 .

Milovanović, Petar, Potočnik, Jelena, Djonic, Danijela, Nikolic, Slobodan, Živković, Vladimir, Đurić, Marija, Rakočević, Zlatko Lj., "Age-related deterioration in trabecular bone mechanical properties at material level: Nanoindentation study of the femoral neck in women by using AFM" in Experimental Gerontology, 47, no. 2 (2012):154-159,
https://doi.org/10.1016/j.exger.2011.11.011 . .

TY  - JOUR
AU  - Milovanović, Petar
AU  - Đurić, Marija
AU  - Rakočević, Zlatko Lj.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5066
AB  - There is an increasing interest in bone nano-structure, the ultimate goal being to reveal the basis of age-related bone fragility. In this study, power spectral density (PSD) data and fractal dimensions of the mineralized bone matrix were extracted from atomic force microscope topography images of the femoral neck trabeculae. The aim was to evaluate age-dependent differences in the mineralized matrix of human bone and to consider whether these advanced nano-descriptors might be linked to decreased bone remodeling observed by some authors and age-related decline in bone mechanical competence. The investigated bone specimens belonged to a group of young adult women (n = 5, age: 2040 years) and a group of elderly women (n = 5, age: 7095 years) without bone diseases. PSD graphs showed the roughness density distribution in relation to spatial frequency. In all cases, there was a fairly linear decrease in magnitude of the power spectra with increasing spatial frequencies. The PSD slope was steeper in elderly individuals (-2.374 vs. -2.066), suggesting the dominance of larger surface morphological features. Fractal dimension of the mineralized bone matrix showed a significant negative trend with advanced age, declining from 2.467 in young individuals to 2.313 in the elderly (r = 0.65, P = 0.04). Higher fractal dimension in young women reflects domination of smaller mineral grains, which is compatible with the more freshly remodeled structure. In contrast, the surface patterns in elderly individuals were indicative of older tissue age. Lower roughness and reduced structural complexity (decreased fractal dimension) of the interfibrillar bone matrix in the elderly suggest a decline in bone toughness, which explains why aged bone is more brittle and prone to fractures.
T2  - Journal of Anatomy
T1  - Age-dependence of power spectral density and fractal dimension of bone mineralized matrix in atomic force microscope topography images: potential correlates of bone tissue age and bone fragility in female femoral neck trabeculae
VL  - 221
IS  - 5
SP  - 427
EP  - 433
DO  - 10.1111/j.1469-7580.2012.01556.x
ER  - 

@article{
author = "Milovanović, Petar and Đurić, Marija and Rakočević, Zlatko Lj.",
year = "2012",
abstract = "There is an increasing interest in bone nano-structure, the ultimate goal being to reveal the basis of age-related bone fragility. In this study, power spectral density (PSD) data and fractal dimensions of the mineralized bone matrix were extracted from atomic force microscope topography images of the femoral neck trabeculae. The aim was to evaluate age-dependent differences in the mineralized matrix of human bone and to consider whether these advanced nano-descriptors might be linked to decreased bone remodeling observed by some authors and age-related decline in bone mechanical competence. The investigated bone specimens belonged to a group of young adult women (n = 5, age: 2040 years) and a group of elderly women (n = 5, age: 7095 years) without bone diseases. PSD graphs showed the roughness density distribution in relation to spatial frequency. In all cases, there was a fairly linear decrease in magnitude of the power spectra with increasing spatial frequencies. The PSD slope was steeper in elderly individuals (-2.374 vs. -2.066), suggesting the dominance of larger surface morphological features. Fractal dimension of the mineralized bone matrix showed a significant negative trend with advanced age, declining from 2.467 in young individuals to 2.313 in the elderly (r = 0.65, P = 0.04). Higher fractal dimension in young women reflects domination of smaller mineral grains, which is compatible with the more freshly remodeled structure. In contrast, the surface patterns in elderly individuals were indicative of older tissue age. Lower roughness and reduced structural complexity (decreased fractal dimension) of the interfibrillar bone matrix in the elderly suggest a decline in bone toughness, which explains why aged bone is more brittle and prone to fractures.",
journal = "Journal of Anatomy",
title = "Age-dependence of power spectral density and fractal dimension of bone mineralized matrix in atomic force microscope topography images: potential correlates of bone tissue age and bone fragility in female femoral neck trabeculae",
volume = "221",
number = "5",
pages = "427-433",
doi = "10.1111/j.1469-7580.2012.01556.x"
}

Milovanović, P., Đurić, M.,& Rakočević, Z. Lj.. (2012). Age-dependence of power spectral density and fractal dimension of bone mineralized matrix in atomic force microscope topography images: potential correlates of bone tissue age and bone fragility in female femoral neck trabeculae. in Journal of Anatomy, 221(5), 427-433.
https://doi.org/10.1111/j.1469-7580.2012.01556.x

Milovanović P, Đurić M, Rakočević ZL. Age-dependence of power spectral density and fractal dimension of bone mineralized matrix in atomic force microscope topography images: potential correlates of bone tissue age and bone fragility in female femoral neck trabeculae. in Journal of Anatomy. 2012;221(5):427-433.
doi:10.1111/j.1469-7580.2012.01556.x .

Milovanović, Petar, Đurić, Marija, Rakočević, Zlatko Lj., "Age-dependence of power spectral density and fractal dimension of bone mineralized matrix in atomic force microscope topography images: potential correlates of bone tissue age and bone fragility in female femoral neck trabeculae" in Journal of Anatomy, 221, no. 5 (2012):427-433,
https://doi.org/10.1111/j.1469-7580.2012.01556.x . .

TY  - JOUR
AU  - Milovanović, Petar
AU  - Potočnik, Jelena
AU  - Stoiljković, Milovan
AU  - Djonic, Danijela
AU  - Nikolic, Slobodan
AU  - Nešković, Olivera M.
AU  - Đurić, Marija
AU  - Rakočević, Zlatko Lj.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4456
AB  - Despite interest in investigating age-related hip fractures, the determinants of decreased bone strength in advanced age are not clear enough. Hitherto it has been obscure how the aging process affects the femoral neck nanostructure and composition, particularly in the lateral subregion of the femoral neck, which is considered as a fracture-initiating site. The femoral bone samples used in this study were obtained at autopsy in 10 women without skeletal disease (five younger: aged 20-40 years, and five elderly: aged 73-94 years). Atomic force microscopy (AFM) was applied to explore the mineral grain size in situ in young vs. old trabecular bone samples from the lateral femoral neck. The chemical compositions of the samples were determined using inductively coupled plasma optical emission spectroscopy and direct current argon arc plasma optical emission spectrometry. Our AFM study revealed differences in trabecular bone nanostructure between young and elderly women. The mineral grain size in the trabeculae of the old women was larger than that in the young (median: 95 vs. 59 nm), with a particular bimodal distribution: 45% were small grains (similar to the young) and the rest were larger. Since chemical analyses showed that levels of calcium and phosphorus were unchanged with age, our study suggests that during aging the existing bone mineral is reorganized and forms larger aggregates. Given the mechanical disadvantage of large-grained structures (decreased material strength), the observed nanostructural differences contribute to our understanding of the increased fragility of the lateral femoral neck in aged females. Moreover, increasing data on mineral grains in natural bone is essential for advancing calcium-phosphate ceramics for bone tissue replacement. (C) 2011 Acts Materialia Inc. Published by Elsevier Ltd. All rights reserved.
T2  - Acta Biomaterialia
T1  - Nanostructure and mineral composition of trabecular bone in the lateral femoral neck: Implications for bone fragility in elderly women
VL  - 7
IS  - 9
SP  - 3446
EP  - 3451
DO  - 10.1016/j.actbio.2011.05.028
ER  - 

@article{
author = "Milovanović, Petar and Potočnik, Jelena and Stoiljković, Milovan and Djonic, Danijela and Nikolic, Slobodan and Nešković, Olivera M. and Đurić, Marija and Rakočević, Zlatko Lj.",
year = "2011",
abstract = "Despite interest in investigating age-related hip fractures, the determinants of decreased bone strength in advanced age are not clear enough. Hitherto it has been obscure how the aging process affects the femoral neck nanostructure and composition, particularly in the lateral subregion of the femoral neck, which is considered as a fracture-initiating site. The femoral bone samples used in this study were obtained at autopsy in 10 women without skeletal disease (five younger: aged 20-40 years, and five elderly: aged 73-94 years). Atomic force microscopy (AFM) was applied to explore the mineral grain size in situ in young vs. old trabecular bone samples from the lateral femoral neck. The chemical compositions of the samples were determined using inductively coupled plasma optical emission spectroscopy and direct current argon arc plasma optical emission spectrometry. Our AFM study revealed differences in trabecular bone nanostructure between young and elderly women. The mineral grain size in the trabeculae of the old women was larger than that in the young (median: 95 vs. 59 nm), with a particular bimodal distribution: 45% were small grains (similar to the young) and the rest were larger. Since chemical analyses showed that levels of calcium and phosphorus were unchanged with age, our study suggests that during aging the existing bone mineral is reorganized and forms larger aggregates. Given the mechanical disadvantage of large-grained structures (decreased material strength), the observed nanostructural differences contribute to our understanding of the increased fragility of the lateral femoral neck in aged females. Moreover, increasing data on mineral grains in natural bone is essential for advancing calcium-phosphate ceramics for bone tissue replacement. (C) 2011 Acts Materialia Inc. Published by Elsevier Ltd. All rights reserved.",
journal = "Acta Biomaterialia",
title = "Nanostructure and mineral composition of trabecular bone in the lateral femoral neck: Implications for bone fragility in elderly women",
volume = "7",
number = "9",
pages = "3446-3451",
doi = "10.1016/j.actbio.2011.05.028"
}

Milovanović, P., Potočnik, J., Stoiljković, M., Djonic, D., Nikolic, S., Nešković, O. M., Đurić, M.,& Rakočević, Z. Lj.. (2011). Nanostructure and mineral composition of trabecular bone in the lateral femoral neck: Implications for bone fragility in elderly women. in Acta Biomaterialia, 7(9), 3446-3451.
https://doi.org/10.1016/j.actbio.2011.05.028

Milovanović P, Potočnik J, Stoiljković M, Djonic D, Nikolic S, Nešković OM, Đurić M, Rakočević ZL. Nanostructure and mineral composition of trabecular bone in the lateral femoral neck: Implications for bone fragility in elderly women. in Acta Biomaterialia. 2011;7(9):3446-3451.
doi:10.1016/j.actbio.2011.05.028 .

Milovanović, Petar, Potočnik, Jelena, Stoiljković, Milovan, Djonic, Danijela, Nikolic, Slobodan, Nešković, Olivera M., Đurić, Marija, Rakočević, Zlatko Lj., "Nanostructure and mineral composition of trabecular bone in the lateral femoral neck: Implications for bone fragility in elderly women" in Acta Biomaterialia, 7, no. 9 (2011):3446-3451,
https://doi.org/10.1016/j.actbio.2011.05.028 . .