TY  - JOUR
AU  - Petrović, Marija
AU  - Popović, Suzana
AU  - Baskić, Dejan
AU  - Todorović, Miloš
AU  - Đurđević, Predrag
AU  - Ristić-Fira, Aleksandra
AU  - Keta, Otilija D.
AU  - Petković, Vladana
AU  - Korićanac, Lela
AU  - Stojković, Danijela
AU  - Jevtić, Verica
AU  - Trifunović, Srećko
AU  - Todorović, Danijela
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9631
AB  - Aim: Newly synthesized platinum(IV) complexes with ethylenediamine-N,N’-diacetate ligands (EDDA-type) (butyl-Pt and pentyl-Pt) were investigated against two cancer (A549 lung, and HTB 140 melanoma) and one non-cancerous (MRC-5 embryonic lung fibroblast) human cell lines. Materials and Methods: The effects of these agents were compared with those of cisplatin after 6-, 24- and 48-h treatment. Sulforhodamine-B (SRB) assay was performed to estimate the cytotoxic effect, while the inhibitory effect on cell proliferation was measured using 5-bromo-2,-deoxyuridine (BrdU) incorporation assay. Cell cycle analysis was performed by flow cytometry. Type of cell death induced by these agents was determined by electrophoretic analysis of DNA, flow cytometry and by western blot analysis of proteins involved in induction of apoptosis. The effects of gamma irradiation, alone and in combination with platinum-based compounds, were examined by clonogenic and SRB assays. Results: All examined platinum-based compounds had inhibitory and antiproliferative effects on A549 cells, but not on HTB140 and MRC-5 cells. Butyl-Pt, pentyl-Pt and cisplatin arrested the cell cycle in the S-phase and induced apoptotic cell death via regulation of expression of B-cell lymphoma 2 (BCL2) and BCL2-associated X (BAX) proteins. Platinum-based compounds increased the sensitivity of A549 cells to gamma irradiation. Butyl-Pt and pentyl-Pt showed better antitumour effects against A549 cells than did cisplatin, by interfering in cell proliferation and the cell cycle, and by triggering apoptosis. Conclusion: The effects of gamma irradiation on tumour cells may be amplified by pre-treatment of cells with platinum-based compounds.
T2  - Anticancer Research
T1  - The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro
VL  - 40
IS  - 9
SP  - 5001
EP  - 5013
DO  - 10.21873/anticanres.14503
ER  - 

@article{
author = "Petrović, Marija and Popović, Suzana and Baskić, Dejan and Todorović, Miloš and Đurđević, Predrag and Ristić-Fira, Aleksandra and Keta, Otilija D. and Petković, Vladana and Korićanac, Lela and Stojković, Danijela and Jevtić, Verica and Trifunović, Srećko and Todorović, Danijela",
year = "2020",
abstract = "Aim: Newly synthesized platinum(IV) complexes with ethylenediamine-N,N’-diacetate ligands (EDDA-type) (butyl-Pt and pentyl-Pt) were investigated against two cancer (A549 lung, and HTB 140 melanoma) and one non-cancerous (MRC-5 embryonic lung fibroblast) human cell lines. Materials and Methods: The effects of these agents were compared with those of cisplatin after 6-, 24- and 48-h treatment. Sulforhodamine-B (SRB) assay was performed to estimate the cytotoxic effect, while the inhibitory effect on cell proliferation was measured using 5-bromo-2,-deoxyuridine (BrdU) incorporation assay. Cell cycle analysis was performed by flow cytometry. Type of cell death induced by these agents was determined by electrophoretic analysis of DNA, flow cytometry and by western blot analysis of proteins involved in induction of apoptosis. The effects of gamma irradiation, alone and in combination with platinum-based compounds, were examined by clonogenic and SRB assays. Results: All examined platinum-based compounds had inhibitory and antiproliferative effects on A549 cells, but not on HTB140 and MRC-5 cells. Butyl-Pt, pentyl-Pt and cisplatin arrested the cell cycle in the S-phase and induced apoptotic cell death via regulation of expression of B-cell lymphoma 2 (BCL2) and BCL2-associated X (BAX) proteins. Platinum-based compounds increased the sensitivity of A549 cells to gamma irradiation. Butyl-Pt and pentyl-Pt showed better antitumour effects against A549 cells than did cisplatin, by interfering in cell proliferation and the cell cycle, and by triggering apoptosis. Conclusion: The effects of gamma irradiation on tumour cells may be amplified by pre-treatment of cells with platinum-based compounds.",
journal = "Anticancer Research",
title = "The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro",
volume = "40",
number = "9",
pages = "5001-5013",
doi = "10.21873/anticanres.14503"
}

Petrović, M., Popović, S., Baskić, D., Todorović, M., Đurđević, P., Ristić-Fira, A., Keta, O. D., Petković, V., Korićanac, L., Stojković, D., Jevtić, V., Trifunović, S.,& Todorović, D.. (2020). The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro. in Anticancer Research, 40(9), 5001-5013.
https://doi.org/10.21873/anticanres.14503

Petrović M, Popović S, Baskić D, Todorović M, Đurđević P, Ristić-Fira A, Keta OD, Petković V, Korićanac L, Stojković D, Jevtić V, Trifunović S, Todorović D. The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro. in Anticancer Research. 2020;40(9):5001-5013.
doi:10.21873/anticanres.14503 .

Petrović, Marija, Popović, Suzana, Baskić, Dejan, Todorović, Miloš, Đurđević, Predrag, Ristić-Fira, Aleksandra, Keta, Otilija D., Petković, Vladana, Korićanac, Lela, Stojković, Danijela, Jevtić, Verica, Trifunović, Srećko, Todorović, Danijela, "The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro" in Anticancer Research, 40, no. 9 (2020):5001-5013,
https://doi.org/10.21873/anticanres.14503 . .

TY  - JOUR
AU  - Joksimović, Nenad
AU  - Petronijević, Jelena
AU  - Janković, Nenad Ž.
AU  - Baskić, Dejan
AU  - Popović, Suzana Lj.
AU  - Todorović, Danijela V.
AU  - Matić, Sanja Lj.
AU  - Bogdanović, Goran A.
AU  - Vraneš, Milan
AU  - Tot, Aleksandar
AU  - Bugarčić, Zorica M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8173
AB  - In order to make a progress in discovering a new agents for chemotherapy with improved properties and bearing in mind the fact that substituted 3-hydroxy-3-pyrrolin-2-ones belong to a class of biologically active compounds, series of novel 1,5-diaryl-4-(2-thienylcarbonyl)-3-hydroxy-3-pyrrolin-2-ones were synthesized and characterized by spectral (UV–Vis, IR, NMR, ESI-MS), X-ray and elemental analysis. All compounds were examined for their cytotoxic effect on human cancer cell lines HeLa and MDA-MB 231 and normal fibroblasts (MRC-5). Four compounds, 3-hydroxy-1-(p-tolyl)-4-(2-thienylcarbonyl)-5-(4-chlorophenyl)-2,5-dihydro-1H-pyrrol-2-one (D10), 3-hydroxy-1-(3-nitrophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D13), 3-hydroxy-1-(4-nitrophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D14), and 3-hydroxy-1-(4-chlorophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D15), that showed the highest cytotoxicity against malignant cells and the best selectivity towards normal cells were selected for further experiments. Results obtained by investigating mechanisms of cytotoxic activity suggest that selected 3-hydroxy-3-pyrrolin-2-one derivatives in HeLa cells induce apoptosis that is associated with S phase arrest (D13, D15, and D10) or unrelated to cell cycle distribution (D14). Additionally, to better understand their suitability for potential use as anticancer medicaments we studied the interactions between biomacromolecules (DNA or BSA) and D13 and D15. The results indicated that D13 and D15 have great affinity to displace EB from the EB-DNA complex through intercalation [K sv = (3.7 ± 0.1) and (3.4 ± 0.1) × 10 3 M −1 , respectively], an intercalative mode also confirmed through viscosity measurements. K a values, obtained as result of fluorescence titration of BSA with D13 and D15 [K a = (4.2 ± 0.2) and (2.6 ± 0.2) × 10 5 M, respectively], support the fact that a significant amount of the tested compounds could be transported and distributed through the cells. In addition, by DNA and BSA molecular docking study for D13, D14 and D15 is determined and predicted the binding mode and the interaction region. © 2019 Elsevier Inc.
T2  - Bioorganic Chemistry
T1  - Synthesis, characterization, anticancer evaluation and mechanisms of cytotoxic activity of novel 3-hydroxy-3-pyrrolin-2-ones bearing thenoyl fragment: DNA, BSA interactions and molecular docking study
VL  - 88
SP  - 102954
DO  - 10.1016/j.bioorg.2019.102954
ER  - 

@article{
author = "Joksimović, Nenad and Petronijević, Jelena and Janković, Nenad Ž. and Baskić, Dejan and Popović, Suzana Lj. and Todorović, Danijela V. and Matić, Sanja Lj. and Bogdanović, Goran A. and Vraneš, Milan and Tot, Aleksandar and Bugarčić, Zorica M.",
year = "2019",
abstract = "In order to make a progress in discovering a new agents for chemotherapy with improved properties and bearing in mind the fact that substituted 3-hydroxy-3-pyrrolin-2-ones belong to a class of biologically active compounds, series of novel 1,5-diaryl-4-(2-thienylcarbonyl)-3-hydroxy-3-pyrrolin-2-ones were synthesized and characterized by spectral (UV–Vis, IR, NMR, ESI-MS), X-ray and elemental analysis. All compounds were examined for their cytotoxic effect on human cancer cell lines HeLa and MDA-MB 231 and normal fibroblasts (MRC-5). Four compounds, 3-hydroxy-1-(p-tolyl)-4-(2-thienylcarbonyl)-5-(4-chlorophenyl)-2,5-dihydro-1H-pyrrol-2-one (D10), 3-hydroxy-1-(3-nitrophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D13), 3-hydroxy-1-(4-nitrophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D14), and 3-hydroxy-1-(4-chlorophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D15), that showed the highest cytotoxicity against malignant cells and the best selectivity towards normal cells were selected for further experiments. Results obtained by investigating mechanisms of cytotoxic activity suggest that selected 3-hydroxy-3-pyrrolin-2-one derivatives in HeLa cells induce apoptosis that is associated with S phase arrest (D13, D15, and D10) or unrelated to cell cycle distribution (D14). Additionally, to better understand their suitability for potential use as anticancer medicaments we studied the interactions between biomacromolecules (DNA or BSA) and D13 and D15. The results indicated that D13 and D15 have great affinity to displace EB from the EB-DNA complex through intercalation [K sv = (3.7 ± 0.1) and (3.4 ± 0.1) × 10 3 M −1 , respectively], an intercalative mode also confirmed through viscosity measurements. K a values, obtained as result of fluorescence titration of BSA with D13 and D15 [K a = (4.2 ± 0.2) and (2.6 ± 0.2) × 10 5 M, respectively], support the fact that a significant amount of the tested compounds could be transported and distributed through the cells. In addition, by DNA and BSA molecular docking study for D13, D14 and D15 is determined and predicted the binding mode and the interaction region. © 2019 Elsevier Inc.",
journal = "Bioorganic Chemistry",
title = "Synthesis, characterization, anticancer evaluation and mechanisms of cytotoxic activity of novel 3-hydroxy-3-pyrrolin-2-ones bearing thenoyl fragment: DNA, BSA interactions and molecular docking study",
volume = "88",
pages = "102954",
doi = "10.1016/j.bioorg.2019.102954"
}

Joksimović, N., Petronijević, J., Janković, N. Ž., Baskić, D., Popović, S. Lj., Todorović, D. V., Matić, S. Lj., Bogdanović, G. A., Vraneš, M., Tot, A.,& Bugarčić, Z. M.. (2019). Synthesis, characterization, anticancer evaluation and mechanisms of cytotoxic activity of novel 3-hydroxy-3-pyrrolin-2-ones bearing thenoyl fragment: DNA, BSA interactions and molecular docking study. in Bioorganic Chemistry, 88, 102954.
https://doi.org/10.1016/j.bioorg.2019.102954

Joksimović N, Petronijević J, Janković NŽ, Baskić D, Popović SL, Todorović DV, Matić SL, Bogdanović GA, Vraneš M, Tot A, Bugarčić ZM. Synthesis, characterization, anticancer evaluation and mechanisms of cytotoxic activity of novel 3-hydroxy-3-pyrrolin-2-ones bearing thenoyl fragment: DNA, BSA interactions and molecular docking study. in Bioorganic Chemistry. 2019;88:102954.
doi:10.1016/j.bioorg.2019.102954 .

Joksimović, Nenad, Petronijević, Jelena, Janković, Nenad Ž., Baskić, Dejan, Popović, Suzana Lj., Todorović, Danijela V., Matić, Sanja Lj., Bogdanović, Goran A., Vraneš, Milan, Tot, Aleksandar, Bugarčić, Zorica M., "Synthesis, characterization, anticancer evaluation and mechanisms of cytotoxic activity of novel 3-hydroxy-3-pyrrolin-2-ones bearing thenoyl fragment: DNA, BSA interactions and molecular docking study" in Bioorganic Chemistry, 88 (2019):102954,
https://doi.org/10.1016/j.bioorg.2019.102954 . .

TY  - JOUR
AU  - Joksimović, Nenad
AU  - Petronijević, Jelena
AU  - Janković, Nenad Ž.
AU  - Baskić, Dejan
AU  - Popović, Suzana Lj.
AU  - Todorović, Danijela V.
AU  - Matić, Sanja Lj.
AU  - Bogdanović, Goran A.
AU  - Vraneš, Milan
AU  - Tot, Aleksandar
AU  - Bugarčić, Zorica M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8186
AB  - In order to make a progress in discovering a new agents for chemotherapy with improved properties and bearing in mind the fact that substituted 3-hydroxy-3-pyrrolin-2-ones belong to a class of biologically active compounds, series of novel 1,5-diaryl-4-(2-thienylcarbonyl)-3-hydroxy-3-pyrrolin-2-ones were synthesized and characterized by spectral (UV–Vis, IR, NMR, ESI-MS), X-ray and elemental analysis. All compounds were examined for their cytotoxic effect on human cancer cell lines HeLa and MDA-MB 231 and normal fibroblasts (MRC-5). Four compounds, 3-hydroxy-1-(p-tolyl)-4-(2-thienylcarbonyl)-5-(4-chlorophenyl)-2,5-dihydro-1H-pyrrol-2-one (D10), 3-hydroxy-1-(3-nitrophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D13), 3-hydroxy-1-(4-nitrophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D14), and 3-hydroxy-1-(4-chlorophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D15), that showed the highest cytotoxicity against malignant cells and the best selectivity towards normal cells were selected for further experiments. Results obtained by investigating mechanisms of cytotoxic activity suggest that selected 3-hydroxy-3-pyrrolin-2-one derivatives in HeLa cells induce apoptosis that is associated with S phase arrest (D13, D15, and D10) or unrelated to cell cycle distribution (D14). Additionally, to better understand their suitability for potential use as anticancer medicaments we studied the interactions between biomacromolecules (DNA or BSA) and D13 and D15. The results indicated that D13 and D15 have great affinity to displace EB from the EB-DNA complex through intercalation [K sv = (3.7 ± 0.1) and (3.4 ± 0.1) × 10 3 M −1 , respectively], an intercalative mode also confirmed through viscosity measurements. K a values, obtained as result of fluorescence titration of BSA with D13 and D15 [K a = (4.2 ± 0.2) and (2.6 ± 0.2) × 10 5 M, respectively], support the fact that a significant amount of the tested compounds could be transported and distributed through the cells. In addition, by DNA and BSA molecular docking study for D13, D14 and D15 is determined and predicted the binding mode and the interaction region. © 2019 Elsevier Inc.
T2  - Bioorganic Chemistry
T1  - Synthesis, characterization, anticancer evaluation and mechanisms of cytotoxic activity of novel 3-hydroxy-3-pyrrolin-2-ones bearing thenoyl fragment: DNA, BSA interactions and molecular docking study
VL  - 88
SP  - 102954
DO  - 10.1016/j.bioorg.2019.102954
ER  - 

@article{
author = "Joksimović, Nenad and Petronijević, Jelena and Janković, Nenad Ž. and Baskić, Dejan and Popović, Suzana Lj. and Todorović, Danijela V. and Matić, Sanja Lj. and Bogdanović, Goran A. and Vraneš, Milan and Tot, Aleksandar and Bugarčić, Zorica M.",
year = "2019",
abstract = "In order to make a progress in discovering a new agents for chemotherapy with improved properties and bearing in mind the fact that substituted 3-hydroxy-3-pyrrolin-2-ones belong to a class of biologically active compounds, series of novel 1,5-diaryl-4-(2-thienylcarbonyl)-3-hydroxy-3-pyrrolin-2-ones were synthesized and characterized by spectral (UV–Vis, IR, NMR, ESI-MS), X-ray and elemental analysis. All compounds were examined for their cytotoxic effect on human cancer cell lines HeLa and MDA-MB 231 and normal fibroblasts (MRC-5). Four compounds, 3-hydroxy-1-(p-tolyl)-4-(2-thienylcarbonyl)-5-(4-chlorophenyl)-2,5-dihydro-1H-pyrrol-2-one (D10), 3-hydroxy-1-(3-nitrophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D13), 3-hydroxy-1-(4-nitrophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D14), and 3-hydroxy-1-(4-chlorophenyl)-4-(2-thienylcarbonyl)-5-(4-(benzyloxy)phenyl)-2,5-dihydro-1H-pyrrol-2-one (D15), that showed the highest cytotoxicity against malignant cells and the best selectivity towards normal cells were selected for further experiments. Results obtained by investigating mechanisms of cytotoxic activity suggest that selected 3-hydroxy-3-pyrrolin-2-one derivatives in HeLa cells induce apoptosis that is associated with S phase arrest (D13, D15, and D10) or unrelated to cell cycle distribution (D14). Additionally, to better understand their suitability for potential use as anticancer medicaments we studied the interactions between biomacromolecules (DNA or BSA) and D13 and D15. The results indicated that D13 and D15 have great affinity to displace EB from the EB-DNA complex through intercalation [K sv = (3.7 ± 0.1) and (3.4 ± 0.1) × 10 3 M −1 , respectively], an intercalative mode also confirmed through viscosity measurements. K a values, obtained as result of fluorescence titration of BSA with D13 and D15 [K a = (4.2 ± 0.2) and (2.6 ± 0.2) × 10 5 M, respectively], support the fact that a significant amount of the tested compounds could be transported and distributed through the cells. In addition, by DNA and BSA molecular docking study for D13, D14 and D15 is determined and predicted the binding mode and the interaction region. © 2019 Elsevier Inc.",
journal = "Bioorganic Chemistry",
title = "Synthesis, characterization, anticancer evaluation and mechanisms of cytotoxic activity of novel 3-hydroxy-3-pyrrolin-2-ones bearing thenoyl fragment: DNA, BSA interactions and molecular docking study",
volume = "88",
pages = "102954",
doi = "10.1016/j.bioorg.2019.102954"
}

Joksimović, N., Petronijević, J., Janković, N. Ž., Baskić, D., Popović, S. Lj., Todorović, D. V., Matić, S. Lj., Bogdanović, G. A., Vraneš, M., Tot, A.,& Bugarčić, Z. M.. (2019). Synthesis, characterization, anticancer evaluation and mechanisms of cytotoxic activity of novel 3-hydroxy-3-pyrrolin-2-ones bearing thenoyl fragment: DNA, BSA interactions and molecular docking study. in Bioorganic Chemistry, 88, 102954.
https://doi.org/10.1016/j.bioorg.2019.102954

Joksimović N, Petronijević J, Janković NŽ, Baskić D, Popović SL, Todorović DV, Matić SL, Bogdanović GA, Vraneš M, Tot A, Bugarčić ZM. Synthesis, characterization, anticancer evaluation and mechanisms of cytotoxic activity of novel 3-hydroxy-3-pyrrolin-2-ones bearing thenoyl fragment: DNA, BSA interactions and molecular docking study. in Bioorganic Chemistry. 2019;88:102954.
doi:10.1016/j.bioorg.2019.102954 .

Joksimović, Nenad, Petronijević, Jelena, Janković, Nenad Ž., Baskić, Dejan, Popović, Suzana Lj., Todorović, Danijela V., Matić, Sanja Lj., Bogdanović, Goran A., Vraneš, Milan, Tot, Aleksandar, Bugarčić, Zorica M., "Synthesis, characterization, anticancer evaluation and mechanisms of cytotoxic activity of novel 3-hydroxy-3-pyrrolin-2-ones bearing thenoyl fragment: DNA, BSA interactions and molecular docking study" in Bioorganic Chemistry, 88 (2019):102954,
https://doi.org/10.1016/j.bioorg.2019.102954 . .

TY  - JOUR
AU  - Ćoćić, Dušan
AU  - Jovanović, Snežana
AU  - Nišavić, Marija
AU  - Baskic, Dejan
AU  - Todorović, Danijela V.
AU  - Popovic, Suzana
AU  - Bugarčić, Živadin D.
AU  - Petrović, Biljana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1758
AB  - Six new dinuclear Pd(II) complexes, [{Pd(2,2-bipy)Cl}(2)(mu-pz)](ClO4)2 (Pd1), [{Pd(dach)Cl}(2)(mu-pz)](ClO4)(2) (Pd2), [{Pd(en)Cl}(2)(mu-pz)] (ClO4)(2) (Pd3), [{Pd(2,2-bipy)Cl}(2)(mu-4,4-bipy)](ClO4)(2) (Pd4), [{Pd(dach)Cl}(2)(mu-4,4-bipy)] (ClO4)(2) (Pd5) and [{Pd(en)Cl-2(mu-4,4-bipy)](ClO4)(2) (Pd6) (where 2,2-bipy = 2,2-bipyridyl, pz = pyrazine, dach = trans-(+/-)-1,2-diaminocyclohexane, en = ethylenediamine, 4,4-bipy = 4,4-bipyridyl) have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR and MALDI-TOF mass spectrometry. The pK(a) values of corresponding diaqua complexes were determined by spectrophotometric pH titration. Substitution reactions with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP) were studied under the pseudo-first order conditions at pH 7.2. Reactions of Pdl with Tu, L-Met and L-Cys were followed by decomposition of complexes, while structures of dinuclear complexes were preserved during the substitution with nitrogen donors. Interactions with calf-thymus DNA (CT DNA) were followed by absorption spectroscopy and fluorescence quenching measurements. All complexes can bind to CT-DNA exhibiting high intrinsic binding constants (K-b = 10(4)-10(5) M-1). Competitive studies with ethidium bromide (EB) have shown that complexes can displace DNA-bound EB. High values of binding constants towards bovine serum albumin protein (BSA) indicate good binding affinity. Finally, all complexes showed moderate to high cytotoxic activity against HeLa (human cervical epithelial carcinoma cell lines) and MDA-MB-231 (human breast epithelial carcinoma cell lines) tumor cell lines inducing apoptotic type cell death, whereas normal fibroblasts were significantly less sensitive. The impact on cell cycle of these cells was distinctive, where Pd4, Pd5 and Pd6 showed the most prominent effect arresting MDA-MB-231 (human lung fibroblast cell lines) cell in G1/S phase of cell cycle.
T2  - Journal of Inorganic Biochemistry
T1  - New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity
VL  - 175
SP  - 67
EP  - 79
DO  - 10.1016/j.jinorgbio.2017.07.009
ER  - 

@article{
author = "Ćoćić, Dušan and Jovanović, Snežana and Nišavić, Marija and Baskic, Dejan and Todorović, Danijela V. and Popovic, Suzana and Bugarčić, Živadin D. and Petrović, Biljana",
year = "2017",
abstract = "Six new dinuclear Pd(II) complexes, [{Pd(2,2-bipy)Cl}(2)(mu-pz)](ClO4)2 (Pd1), [{Pd(dach)Cl}(2)(mu-pz)](ClO4)(2) (Pd2), [{Pd(en)Cl}(2)(mu-pz)] (ClO4)(2) (Pd3), [{Pd(2,2-bipy)Cl}(2)(mu-4,4-bipy)](ClO4)(2) (Pd4), [{Pd(dach)Cl}(2)(mu-4,4-bipy)] (ClO4)(2) (Pd5) and [{Pd(en)Cl-2(mu-4,4-bipy)](ClO4)(2) (Pd6) (where 2,2-bipy = 2,2-bipyridyl, pz = pyrazine, dach = trans-(+/-)-1,2-diaminocyclohexane, en = ethylenediamine, 4,4-bipy = 4,4-bipyridyl) have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR and MALDI-TOF mass spectrometry. The pK(a) values of corresponding diaqua complexes were determined by spectrophotometric pH titration. Substitution reactions with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP) were studied under the pseudo-first order conditions at pH 7.2. Reactions of Pdl with Tu, L-Met and L-Cys were followed by decomposition of complexes, while structures of dinuclear complexes were preserved during the substitution with nitrogen donors. Interactions with calf-thymus DNA (CT DNA) were followed by absorption spectroscopy and fluorescence quenching measurements. All complexes can bind to CT-DNA exhibiting high intrinsic binding constants (K-b = 10(4)-10(5) M-1). Competitive studies with ethidium bromide (EB) have shown that complexes can displace DNA-bound EB. High values of binding constants towards bovine serum albumin protein (BSA) indicate good binding affinity. Finally, all complexes showed moderate to high cytotoxic activity against HeLa (human cervical epithelial carcinoma cell lines) and MDA-MB-231 (human breast epithelial carcinoma cell lines) tumor cell lines inducing apoptotic type cell death, whereas normal fibroblasts were significantly less sensitive. The impact on cell cycle of these cells was distinctive, where Pd4, Pd5 and Pd6 showed the most prominent effect arresting MDA-MB-231 (human lung fibroblast cell lines) cell in G1/S phase of cell cycle.",
journal = "Journal of Inorganic Biochemistry",
title = "New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity",
volume = "175",
pages = "67-79",
doi = "10.1016/j.jinorgbio.2017.07.009"
}

Ćoćić, D., Jovanović, S., Nišavić, M., Baskic, D., Todorović, D. V., Popovic, S., Bugarčić, Ž. D.,& Petrović, B.. (2017). New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity. in Journal of Inorganic Biochemistry, 175, 67-79.
https://doi.org/10.1016/j.jinorgbio.2017.07.009

Ćoćić D, Jovanović S, Nišavić M, Baskic D, Todorović DV, Popovic S, Bugarčić ŽD, Petrović B. New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity. in Journal of Inorganic Biochemistry. 2017;175:67-79.
doi:10.1016/j.jinorgbio.2017.07.009 .

Ćoćić, Dušan, Jovanović, Snežana, Nišavić, Marija, Baskic, Dejan, Todorović, Danijela V., Popovic, Suzana, Bugarčić, Živadin D., Petrović, Biljana, "New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity" in Journal of Inorganic Biochemistry, 175 (2017):67-79,
https://doi.org/10.1016/j.jinorgbio.2017.07.009 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Bulat, Tanja M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1573
AB  - The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied.
T2  - Experimental Biology and Medicine
T1  - Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions
VL  - 242
IS  - 10
SP  - 1015
EP  - 1024
DO  - 10.1177/1535370216669611
ER  - 

@article{
author = "Keta, Otilija D. and Todorović, Danijela V. and Bulat, Tanja M. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2017",
abstract = "The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied.",
journal = "Experimental Biology and Medicine",
title = "Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions",
volume = "242",
number = "10",
pages = "1015-1024",
doi = "10.1177/1535370216669611"
}

Keta, O. D., Todorović, D. V., Bulat, T. M., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2017). Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. in Experimental Biology and Medicine, 242(10), 1015-1024.
https://doi.org/10.1177/1535370216669611

Keta OD, Todorović DV, Bulat TM, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. in Experimental Biology and Medicine. 2017;242(10):1015-1024.
doi:10.1177/1535370216669611 .

Keta, Otilija D., Todorović, Danijela V., Bulat, Tanja M., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions" in Experimental Biology and Medicine, 242, no. 10 (2017):1015-1024,
https://doi.org/10.1177/1535370216669611 . .

TY  - JOUR
AU  - Bulat, Tanja M.
AU  - Keta, Otilija D.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/970
AB  - Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (gamma H2AX). Immunofluorescent staining visualizes formation of gamma H2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of gamma H2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to gamma-rays, in the dose range from 1 to 16 Gy. Radiation effects on the DNA level were analyzed at different time intervals after irradiation by Western blot analysis and immunofluorescence microscopy. Immunochemically stained cells were visualized with two types of microscopes: AxioVision (Zeiss, Germany) microscope, comprising an ApoTome software, and AxioImagerA1 microscope (Zeiss, Germany). Obtained results show that the level of gamma H2AX is time and dose dependent. Immunofluorescence microscopy provided better detection of DSBs for lower irradiation doses, while Western blot analysis was more reliable for higher irradiation doses. AxioVision microscope containing ApoTome software was more suitable for the detection of gamma H2AX foci.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Radiation dose determines the method for quantification of DNA double strand breaks
VL  - 88
IS  - 1
SP  - 127
EP  - 136
DO  - 10.1590/0001-3765201620140553
ER  - 

@article{
author = "Bulat, Tanja M. and Keta, Otilija D. and Korićanac, Lela and Žakula, Jelena and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V.",
year = "2016",
abstract = "Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (gamma H2AX). Immunofluorescent staining visualizes formation of gamma H2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of gamma H2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to gamma-rays, in the dose range from 1 to 16 Gy. Radiation effects on the DNA level were analyzed at different time intervals after irradiation by Western blot analysis and immunofluorescence microscopy. Immunochemically stained cells were visualized with two types of microscopes: AxioVision (Zeiss, Germany) microscope, comprising an ApoTome software, and AxioImagerA1 microscope (Zeiss, Germany). Obtained results show that the level of gamma H2AX is time and dose dependent. Immunofluorescence microscopy provided better detection of DSBs for lower irradiation doses, while Western blot analysis was more reliable for higher irradiation doses. AxioVision microscope containing ApoTome software was more suitable for the detection of gamma H2AX foci.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Radiation dose determines the method for quantification of DNA double strand breaks",
volume = "88",
number = "1",
pages = "127-136",
doi = "10.1590/0001-3765201620140553"
}

Bulat, T. M., Keta, O. D., Korićanac, L., Žakula, J., Petrović, I. M., Ristić-Fira, A.,& Todorović, D. V.. (2016). Radiation dose determines the method for quantification of DNA double strand breaks. in Anais de Academia Brasileira de Ciencias, 88(1), 127-136.
https://doi.org/10.1590/0001-3765201620140553

Bulat TM, Keta OD, Korićanac L, Žakula J, Petrović IM, Ristić-Fira A, Todorović DV. Radiation dose determines the method for quantification of DNA double strand breaks. in Anais de Academia Brasileira de Ciencias. 2016;88(1):127-136.
doi:10.1590/0001-3765201620140553 .

Bulat, Tanja M., Keta, Otilija D., Korićanac, Lela, Žakula, Jelena, Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., "Radiation dose determines the method for quantification of DNA double strand breaks" in Anais de Academia Brasileira de Ciencias, 88, no. 1 (2016):127-136,
https://doi.org/10.1590/0001-3765201620140553 . .

TY  - JOUR
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1290
AB  - Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.
T2  - Indian Journal of Medical Research
T1  - Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells
VL  - 143
SP  - 120
EP  - 128
DO  - 10.4103/0971-5916.191811
ER  - 

@article{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Todorović, Danijela V. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.",
journal = "Indian Journal of Medical Research",
title = "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells",
volume = "143",
pages = "120-128",
doi = "10.4103/0971-5916.191811"
}

Žakula, J., Korićanac, L., Keta, O. D., Todorović, D. V., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2016). Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research, 143, 120-128.
https://doi.org/10.4103/0971-5916.191811

Žakula J, Korićanac L, Keta OD, Todorović DV, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research. 2016;143:120-128.
doi:10.4103/0971-5916.191811 .

Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Todorović, Danijela V., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells" in Indian Journal of Medical Research, 143 (2016):120-128,
https://doi.org/10.4103/0971-5916.191811 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Popović, Nataša M.
AU  - Korićanac, Lela
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5447
AB  - Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.
T2  - Archives of Medical Science
T1  - Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons
VL  - 10
IS  - 3
SP  - 578
EP  - 586
DO  - 10.5114/aoms.2014.43751
ER  - 

@article{
author = "Keta, Otilija D. and Todorović, Danijela V. and Popović, Nataša M. and Korićanac, Lela and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2014",
abstract = "Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.",
journal = "Archives of Medical Science",
title = "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons",
volume = "10",
number = "3",
pages = "578-586",
doi = "10.5114/aoms.2014.43751"
}

Keta, O. D., Todorović, D. V., Popović, N. M., Korićanac, L., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2014). Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. in Archives of Medical Science, 10(3), 578-586.
https://doi.org/10.5114/aoms.2014.43751

Keta OD, Todorović DV, Popović NM, Korićanac L, Cuttone G, Petrović IM, Ristić-Fira A. Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. in Archives of Medical Science. 2014;10(3):578-586.
doi:10.5114/aoms.2014.43751 .

Keta, Otilija D., Todorović, Danijela V., Popović, Nataša M., Korićanac, Lela, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons" in Archives of Medical Science, 10, no. 3 (2014):578-586,
https://doi.org/10.5114/aoms.2014.43751 . .

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4537
AB  - Conventional radiotherapy with X-and gamma-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and (12)C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the radio-resistant human HTB140 melanoma cells. The cells were irradiated with gamma-rays, the 62 MeV protons at the Bragg peak and in the middle of the spread-out Bragg peak (SOBP), as well as with the 62 MeV/u (12)C ions. The doses ranged from 2 to 24 Gy. Cell survival and proliferation were assessed 7 days after irradiation, whereas apoptosis was evaluated after 48 h. The acquired results confirmed the high radio-resistance of cells, showing better effectiveness of protons than gamma-rays. The best efficiency was obtained with (12)C ions due to higher linear energy transfer. All analyzed radiation qualities reduced cell proliferation. The highest proliferation was detected for (12)C ions because of their large killing capacity followed by small induction of reparable lesions. This enabled unharmed cells to preserve proliferative activity. Irradiations with protons and (12)C ions revealed similar moderate pro-apoptotic ability that is in agreement with the level of cellular radio-resistance.
T2  - Physiological Research
T1  - Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons
VL  - 60
SP  - S129
EP  - S135
UR  - https://hdl.handle.net/21.15107/rcub_vinar_4537
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Žakula, Jelena and Keta, Otilija D. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2011",
abstract = "Conventional radiotherapy with X-and gamma-rays is one of the common and effective treatments of cancer. High energy hadrons, i.e., charged particles like protons and (12)C ions, due to their specific physics and radiobiological advantages are increasingly used. In this study, effectiveness of different radiation types is evaluated on the radio-resistant human HTB140 melanoma cells. The cells were irradiated with gamma-rays, the 62 MeV protons at the Bragg peak and in the middle of the spread-out Bragg peak (SOBP), as well as with the 62 MeV/u (12)C ions. The doses ranged from 2 to 24 Gy. Cell survival and proliferation were assessed 7 days after irradiation, whereas apoptosis was evaluated after 48 h. The acquired results confirmed the high radio-resistance of cells, showing better effectiveness of protons than gamma-rays. The best efficiency was obtained with (12)C ions due to higher linear energy transfer. All analyzed radiation qualities reduced cell proliferation. The highest proliferation was detected for (12)C ions because of their large killing capacity followed by small induction of reparable lesions. This enabled unharmed cells to preserve proliferative activity. Irradiations with protons and (12)C ions revealed similar moderate pro-apoptotic ability that is in agreement with the level of cellular radio-resistance.",
journal = "Physiological Research",
title = "Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons",
volume = "60",
pages = "S129-S135",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4537"
}

Ristić-Fira, A., Todorović, D. V., Žakula, J., Keta, O. D., Cirrone, G. A. P., Cuttone, G.,& Petrović, I. M.. (2011). Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons. in Physiological Research, 60, S129-S135.
https://hdl.handle.net/21.15107/rcub_vinar_4537

Ristić-Fira A, Todorović DV, Žakula J, Keta OD, Cirrone GAP, Cuttone G, Petrović IM. Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons. in Physiological Research. 2011;60:S129-S135.
https://hdl.handle.net/21.15107/rcub_vinar_4537 .

Ristić-Fira, Aleksandra, Todorović, Danijela V., Žakula, Jelena, Keta, Otilija D., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Petrović, Ivan M., "Response of Human HTB140 Melanoma Cells to Conventional Radiation and Hadrons" in Physiological Research, 60 (2011):S129-S135,
https://hdl.handle.net/21.15107/rcub_vinar_4537 .

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Valastro, Lucia
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4101
AB  - Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.
T2  - International Journal of Radiation Biology
T1  - Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak
VL  - 86
IS  - 9
SP  - 742
EP  - 751
DO  - 10.3109/09553002.2010.481322
ER  - 

@article{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Valastro, Lucia and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2010",
abstract = "Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.",
journal = "International Journal of Radiation Biology",
title = "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak",
volume = "86",
number = "9",
pages = "742-751",
doi = "10.3109/09553002.2010.481322"
}

Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Korićanac, L., Valastro, L., Cirrone, G. A. P.,& Cuttone, G.. (2010). Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology, 86(9), 742-751.
https://doi.org/10.3109/09553002.2010.481322

Petrović IM, Ristić-Fira A, Todorović DV, Korićanac L, Valastro L, Cirrone GAP, Cuttone G. Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology. 2010;86(9):742-751.
doi:10.3109/09553002.2010.481322 .

Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Valastro, Lucia, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak" in International Journal of Radiation Biology, 86, no. 9 (2010):742-751,
https://doi.org/10.3109/09553002.2010.481322 . .

TY  - JOUR
AU  - Todorović, Danijela V.
AU  - Petrović, Ivan M.
AU  - Todorović, M.
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3573
AB  - The effects of irradiation with gamma rays and protons on HTB140 human melanoma cell morphology and viability were analyzed. Exponentially growing cells were irradiated close to the Bragg peak maximum of the 62-MeV proton beam, as well as with (60)Co gamma rays, with doses ranging from 8 to 24 Gy. The overall cell morphology was unchanged 6 and 48 h after gamma irradiation, also showing a relatively weak cell-inactivation level. After exposure to proton beam, considerable changes in cell morphology followed by stronger cell inactivation were achieved. Proliferation capacity of irradiated cells significantly decreased in both experimental set-ups. Higher ionization level of protons with respect to gamma rays, representing the main physical difference between these two types of radiation, was also revealed on the cell membrane level through larger pro-apoptotic capacity of protons.
T2  - Journal of Microscopy, Oxford
T1  - Early effects of gamma rays and protons on human melanoma cell viability and morphology
VL  - 232
IS  - 3
SP  - 517
EP  - 521
DO  - 10.1111/j.1365-2818.2008.02151.x
ER  - 

@article{
author = "Todorović, Danijela V. and Petrović, Ivan M. and Todorović, M. and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2008",
abstract = "The effects of irradiation with gamma rays and protons on HTB140 human melanoma cell morphology and viability were analyzed. Exponentially growing cells were irradiated close to the Bragg peak maximum of the 62-MeV proton beam, as well as with (60)Co gamma rays, with doses ranging from 8 to 24 Gy. The overall cell morphology was unchanged 6 and 48 h after gamma irradiation, also showing a relatively weak cell-inactivation level. After exposure to proton beam, considerable changes in cell morphology followed by stronger cell inactivation were achieved. Proliferation capacity of irradiated cells significantly decreased in both experimental set-ups. Higher ionization level of protons with respect to gamma rays, representing the main physical difference between these two types of radiation, was also revealed on the cell membrane level through larger pro-apoptotic capacity of protons.",
journal = "Journal of Microscopy, Oxford",
title = "Early effects of gamma rays and protons on human melanoma cell viability and morphology",
volume = "232",
number = "3",
pages = "517-521",
doi = "10.1111/j.1365-2818.2008.02151.x"
}

Todorović, D. V., Petrović, I. M., Todorović, M., Cuttone, G.,& Ristić-Fira, A.. (2008). Early effects of gamma rays and protons on human melanoma cell viability and morphology. in Journal of Microscopy, Oxford, 232(3), 517-521.
https://doi.org/10.1111/j.1365-2818.2008.02151.x

Todorović DV, Petrović IM, Todorović M, Cuttone G, Ristić-Fira A. Early effects of gamma rays and protons on human melanoma cell viability and morphology. in Journal of Microscopy, Oxford. 2008;232(3):517-521.
doi:10.1111/j.1365-2818.2008.02151.x .

Todorović, Danijela V., Petrović, Ivan M., Todorović, M., Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Early effects of gamma rays and protons on human melanoma cell viability and morphology" in Journal of Microscopy, Oxford, 232, no. 3 (2008):517-521,
https://doi.org/10.1111/j.1365-2818.2008.02151.x . .

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Korićanac, Lela
AU  - Todorović, Danijela V.
AU  - Ristić-Fira, Aleksandra
AU  - Valastro, Lucia M.
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6647
AB  - Viability of human HTB140 melanoma cells after being exposed to fotemustine (FM) and dacarbazine (DTIC) as well as to proton irradiation was studied. Effects of 100 and 250 mu M drugs were assessed after incubation of 6, 24, 48, 72, and 96 h. Irradiations were performed with 62 MeV therapeutic protons, delivering to the cell monolayer single doses of 2, 4, 8, 12, and 16 Gy. Viability was evaluated 7 days after irradiation. Inactivation level was estimated using microtetrasolium (MTT) and sulforhodamine B (SRB) assays. Combined effects of each drug and protons, were carried out using the same drug concentrations. Proton doses applied were those used in therapy, that is, 12 and 16 Gy. With the increase of drug concentration or irradiation dose, level of cell inactivation reached approximately 60%, 48 h after drug treatment or 7 days after irradiation at 16 Gy. Considering the rate of drug concentrations used, as well as the level of doses applied, it appears that HTB140 cells are more resistant to proton irradiation than to alkylating agents tested. The combined treatment with FM or DTIC and protons did not show significant changes of cell viability as compared to the effects of single agents. Since the time point for measuring cumulative effects of drug and irradiation was 48 h post irradiation, it seems that the obtained level of viability could be attributed primarily to the effects of drugs.
T2  - Annals of the New York Academy of Sciences
T1  - Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation
VL  - 1095
SP  - 154
EP  - 164
DO  - 10.1196/annals.1397.019
ER  - 

@article{
author = "Petrović, Ivan M. and Korićanac, Lela and Todorović, Danijela V. and Ristić-Fira, Aleksandra and Valastro, Lucia M. and Privitera, Giuseppe and Cuttone, Giacomo",
year = "2007",
abstract = "Viability of human HTB140 melanoma cells after being exposed to fotemustine (FM) and dacarbazine (DTIC) as well as to proton irradiation was studied. Effects of 100 and 250 mu M drugs were assessed after incubation of 6, 24, 48, 72, and 96 h. Irradiations were performed with 62 MeV therapeutic protons, delivering to the cell monolayer single doses of 2, 4, 8, 12, and 16 Gy. Viability was evaluated 7 days after irradiation. Inactivation level was estimated using microtetrasolium (MTT) and sulforhodamine B (SRB) assays. Combined effects of each drug and protons, were carried out using the same drug concentrations. Proton doses applied were those used in therapy, that is, 12 and 16 Gy. With the increase of drug concentration or irradiation dose, level of cell inactivation reached approximately 60%, 48 h after drug treatment or 7 days after irradiation at 16 Gy. Considering the rate of drug concentrations used, as well as the level of doses applied, it appears that HTB140 cells are more resistant to proton irradiation than to alkylating agents tested. The combined treatment with FM or DTIC and protons did not show significant changes of cell viability as compared to the effects of single agents. Since the time point for measuring cumulative effects of drug and irradiation was 48 h post irradiation, it seems that the obtained level of viability could be attributed primarily to the effects of drugs.",
journal = "Annals of the New York Academy of Sciences",
title = "Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation",
volume = "1095",
pages = "154-164",
doi = "10.1196/annals.1397.019"
}

Petrović, I. M., Korićanac, L., Todorović, D. V., Ristić-Fira, A., Valastro, L. M., Privitera, G.,& Cuttone, G.. (2007). Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation. in Annals of the New York Academy of Sciences, 1095, 154-164.
https://doi.org/10.1196/annals.1397.019

Petrović IM, Korićanac L, Todorović DV, Ristić-Fira A, Valastro LM, Privitera G, Cuttone G. Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation. in Annals of the New York Academy of Sciences. 2007;1095:154-164.
doi:10.1196/annals.1397.019 .

Petrović, Ivan M., Korićanac, Lela, Todorović, Danijela V., Ristić-Fira, Aleksandra, Valastro, Lucia M., Privitera, Giuseppe, Cuttone, Giacomo, "Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation" in Annals of the New York Academy of Sciences, 1095 (2007):154-164,
https://doi.org/10.1196/annals.1397.019 . .

TY  - CONF
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Petrović, Ivan M.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Raffaele, Luigi
AU  - Cuttone, Giacomo
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6648
AB  - Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cells decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells.
C3  - Annals of the New York Academy of Sciences
T1  - Response of a human melanoma cell line to low and high ionizing radiation
VL  - 1095
SP  - 165
EP  - 174
DO  - 10.1196/annals.1397.020
ER  - 

@conference{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Petrović, Ivan M. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Raffaele, Luigi and Cuttone, Giacomo",
year = "2007",
abstract = "Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cells decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells.",
journal = "Annals of the New York Academy of Sciences",
title = "Response of a human melanoma cell line to low and high ionizing radiation",
volume = "1095",
pages = "165-174",
doi = "10.1196/annals.1397.020"
}

Ristić-Fira, A., Todorović, D. V., Korićanac, L., Petrović, I. M., Valastro, L. M., Cirrone, G. A. P., Raffaele, L.,& Cuttone, G.. (2007). Response of a human melanoma cell line to low and high ionizing radiation. in Annals of the New York Academy of Sciences, 1095, 165-174.
https://doi.org/10.1196/annals.1397.020

Ristić-Fira A, Todorović DV, Korićanac L, Petrović IM, Valastro LM, Cirrone GAP, Raffaele L, Cuttone G. Response of a human melanoma cell line to low and high ionizing radiation. in Annals of the New York Academy of Sciences. 2007;1095:165-174.
doi:10.1196/annals.1397.020 .

Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Petrović, Ivan M., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Raffaele, Luigi, Cuttone, Giacomo, "Response of a human melanoma cell line to low and high ionizing radiation" in Annals of the New York Academy of Sciences, 1095 (2007):165-174,
https://doi.org/10.1196/annals.1397.020 . .

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3017
AB  - Purpose: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. Materials and methods: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with Co-60 gamma-rays with single doses, ranging from 8-24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. Results: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of similar to 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. Conclusions: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.
T2  - International Journal of Radiation Biology
T1  - Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam
VL  - 82
IS  - 4
SP  - 251
EP  - 265
DO  - 10.1080/09553000600669859
ER  - 

@article{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2006",
abstract = "Purpose: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. Materials and methods: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with Co-60 gamma-rays with single doses, ranging from 8-24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. Results: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of similar to 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. Conclusions: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.",
journal = "International Journal of Radiation Biology",
title = "Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam",
volume = "82",
number = "4",
pages = "251-265",
doi = "10.1080/09553000600669859"
}

Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Valastro, L. M., Cirrone, G. A. P.,& Cuttone, G.. (2006). Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam. in International Journal of Radiation Biology, 82(4), 251-265.
https://doi.org/10.1080/09553000600669859

Petrović IM, Ristić-Fira A, Todorović DV, Valastro LM, Cirrone GAP, Cuttone G. Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam. in International Journal of Radiation Biology. 2006;82(4):251-265.
doi:10.1080/09553000600669859 .

Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam" in International Journal of Radiation Biology, 82, no. 4 (2006):251-265,
https://doi.org/10.1080/09553000600669859 . .

TY  - CONF
AU  - Korićanac, Lela
AU  - Todorović, Danijela V.
AU  - Popović, Nataša M.
AU  - Ristić-Fira, Aleksandra
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9505
AB  - The aim of this study was to investigate the in vitro effects of 8- chloroadenosine 3′, 5′-monophosphate (8-Cl-cAMP) and tiazofurin (TR) on the expression of c-myc gene in B16/F10 and B16/C3 mouse melanoma cells. Exponentially growing cells were treated with 8-Cl-cAMP or TR (5µmol - 25µmol) for 6h and 24h. The level of c-myc expression, estimated by RT-PCR, did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. Similar results were obtained in B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression has shown a significant increase in B16/C3 cells after treatment with TR. Further studies of these agents will lead to better understanding of molecular mechanisms of their action.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Changes of c-myc expression in b16 melanoma cells induced by 8-chloroadenosine-3′, 5′-monophosphate and tiazofurin
VL  - 1
SP  - 413
EP  - 415
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9505
ER  - 

@conference{
author = "Korićanac, Lela and Todorović, Danijela V. and Popović, Nataša M. and Ristić-Fira, Aleksandra",
year = "2004",
abstract = "The aim of this study was to investigate the in vitro effects of 8- chloroadenosine 3′, 5′-monophosphate (8-Cl-cAMP) and tiazofurin (TR) on the expression of c-myc gene in B16/F10 and B16/C3 mouse melanoma cells. Exponentially growing cells were treated with 8-Cl-cAMP or TR (5µmol - 25µmol) for 6h and 24h. The level of c-myc expression, estimated by RT-PCR, did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. Similar results were obtained in B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression has shown a significant increase in B16/C3 cells after treatment with TR. Further studies of these agents will lead to better understanding of molecular mechanisms of their action.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Changes of c-myc expression in b16 melanoma cells induced by 8-chloroadenosine-3′, 5′-monophosphate and tiazofurin",
volume = "1",
pages = "413-415",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9505"
}

Korićanac, L., Todorović, D. V., Popović, N. M.,& Ristić-Fira, A.. (2004). Changes of c-myc expression in b16 melanoma cells induced by 8-chloroadenosine-3′, 5′-monophosphate and tiazofurin. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 413-415.
https://hdl.handle.net/21.15107/rcub_vinar_9505

Korićanac L, Todorović DV, Popović NM, Ristić-Fira A. Changes of c-myc expression in b16 melanoma cells induced by 8-chloroadenosine-3′, 5′-monophosphate and tiazofurin. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:413-415.
https://hdl.handle.net/21.15107/rcub_vinar_9505 .

Korićanac, Lela, Todorović, Danijela V., Popović, Nataša M., Ristić-Fira, Aleksandra, "Changes of c-myc expression in b16 melanoma cells induced by 8-chloroadenosine-3′, 5′-monophosphate and tiazofurin" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):413-415,
https://hdl.handle.net/21.15107/rcub_vinar_9505 .

TY  - JOUR
AU  - Korićanac, Lela
AU  - Todorović, Danijela V.
AU  - Popović, Nataša M.
AU  - Demajo, Miroslav
AU  - Ruždijić, Sabera
AU  - Ristić-Fira, Aleksandra
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6500
AB  - Novel antineoplastic agents, 8-chloroadenosine 3,5-monophosphate (8-Cl-cAMP) and tiazofurin (TR), have been shown to be effective against different malignant cells. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, and apoptosis. The aim of this work was the in vitro study of either 8-Cl-cAMP or TR effects on B16/F10 and B16/C3 mouse melanoma cell growth and cell death. Significant cell growth inhibition was obtained after the application of 8-Cl-cAMP or TR. The presence and number of apoptotic cells was evaluated using agarose gel electrophoresis and flow cytometry. The number of apoptotic nuclei, after treatment with antineoplastic agents, did not significantly change in B16/F10 cells, although it did show a significant increase in B16/C3 cells. The expression of c-myc did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. The same results were obtained in B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression showed a significant increase in B16/C3 cells after treatment with TR. Concerning the effects that the analyzed agents exhibited on melanoma cells and other cancer cells, further preclinical studies of these drugs will potentially lead to better understanding of the molecular mechanisms of their action and finally more efficient therapeutic approaches to malignant diseases.
T2  - Annals of the New York Academy of Sciences
T1  - Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin
VL  - 1030
SP  - 384
EP  - 392
DO  - 10.1196/annals.1329.048
ER  - 

@article{
author = "Korićanac, Lela and Todorović, Danijela V. and Popović, Nataša M. and Demajo, Miroslav and Ruždijić, Sabera and Ristić-Fira, Aleksandra",
year = "2004",
abstract = "Novel antineoplastic agents, 8-chloroadenosine 3,5-monophosphate (8-Cl-cAMP) and tiazofurin (TR), have been shown to be effective against different malignant cells. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, and apoptosis. The aim of this work was the in vitro study of either 8-Cl-cAMP or TR effects on B16/F10 and B16/C3 mouse melanoma cell growth and cell death. Significant cell growth inhibition was obtained after the application of 8-Cl-cAMP or TR. The presence and number of apoptotic cells was evaluated using agarose gel electrophoresis and flow cytometry. The number of apoptotic nuclei, after treatment with antineoplastic agents, did not significantly change in B16/F10 cells, although it did show a significant increase in B16/C3 cells. The expression of c-myc did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. The same results were obtained in B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression showed a significant increase in B16/C3 cells after treatment with TR. Concerning the effects that the analyzed agents exhibited on melanoma cells and other cancer cells, further preclinical studies of these drugs will potentially lead to better understanding of the molecular mechanisms of their action and finally more efficient therapeutic approaches to malignant diseases.",
journal = "Annals of the New York Academy of Sciences",
title = "Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin",
volume = "1030",
pages = "384-392",
doi = "10.1196/annals.1329.048"
}

Korićanac, L., Todorović, D. V., Popović, N. M., Demajo, M., Ruždijić, S.,& Ristić-Fira, A.. (2004). Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin. in Annals of the New York Academy of Sciences, 1030, 384-392.
https://doi.org/10.1196/annals.1329.048

Korićanac L, Todorović DV, Popović NM, Demajo M, Ruždijić S, Ristić-Fira A. Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin. in Annals of the New York Academy of Sciences. 2004;1030:384-392.
doi:10.1196/annals.1329.048 .

Korićanac, Lela, Todorović, Danijela V., Popović, Nataša M., Demajo, Miroslav, Ruždijić, Sabera, Ristić-Fira, Aleksandra, "Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin" in Annals of the New York Academy of Sciences, 1030 (2004):384-392,
https://doi.org/10.1196/annals.1329.048 . .

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Vujčić, Miroslava T.
AU  - Demajo, Miroslav
AU  - Sabini, G
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2829
AB  - The effects of single irradiation with gamma rays and protons on HTB63 human melanoma cell growth were compared. The exponentially growing cells were irradiated with gamma rays or protons using doses ranging from 2-20 Gy. At 48 It of post-irradiation incubation under standard conditions, cell survival and induction of apoptotic cell death were examined. The best effect of the single irradiation with gamma rays was the reduction of cell growth by up to 26% (p=0.048, irradiation vs. control), obtained using the dose of 16 Gy. The same doses of proton irradiation, having energy at the target of 22.6 MeV, significantly inhibited melanoma cell growth. Doses of 12 and 16 Gy of protons provoked growth inhibition of 48.9% (p=0.003, irradiation vs. control) and 51.2% (p=0.012, irradiation vs. control) respectively. Irradiation with 12 and 16 Gy protons, compared to the effects of the same doses of gamma rays, significantly reduced melanoma cell growth (p=0.015 and p=0.028, protons vs. gamma rays, respectively). Estimated RBEs for growth inhibition of HTB63 cells ranged from 1.02 to 1.45. The electrophoretical analyses of DNA samples and flow cytometric evaluation have shown a low percentage of apoptotic cells after both types of irradiation. The better inhibitory effect achieved by protons in contrast to gamma rays, can be explained considering specific physical properties of protons, especially taking into account the highly localized energy deposition (high LET).
T2  - Oncology Reports
T1  - Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays
VL  - 12
IS  - 6
SP  - 1323
EP  - 1328
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2829
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Petrović, Ivan M. and Todorović, Danijela V. and Korićanac, Lela and Vujčić, Miroslava T. and Demajo, Miroslav and Sabini, G and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2004",
abstract = "The effects of single irradiation with gamma rays and protons on HTB63 human melanoma cell growth were compared. The exponentially growing cells were irradiated with gamma rays or protons using doses ranging from 2-20 Gy. At 48 It of post-irradiation incubation under standard conditions, cell survival and induction of apoptotic cell death were examined. The best effect of the single irradiation with gamma rays was the reduction of cell growth by up to 26% (p=0.048, irradiation vs. control), obtained using the dose of 16 Gy. The same doses of proton irradiation, having energy at the target of 22.6 MeV, significantly inhibited melanoma cell growth. Doses of 12 and 16 Gy of protons provoked growth inhibition of 48.9% (p=0.003, irradiation vs. control) and 51.2% (p=0.012, irradiation vs. control) respectively. Irradiation with 12 and 16 Gy protons, compared to the effects of the same doses of gamma rays, significantly reduced melanoma cell growth (p=0.015 and p=0.028, protons vs. gamma rays, respectively). Estimated RBEs for growth inhibition of HTB63 cells ranged from 1.02 to 1.45. The electrophoretical analyses of DNA samples and flow cytometric evaluation have shown a low percentage of apoptotic cells after both types of irradiation. The better inhibitory effect achieved by protons in contrast to gamma rays, can be explained considering specific physical properties of protons, especially taking into account the highly localized energy deposition (high LET).",
journal = "Oncology Reports",
title = "Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays",
volume = "12",
number = "6",
pages = "1323-1328",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2829"
}

Ristić-Fira, A., Petrović, I. M., Todorović, D. V., Korićanac, L., Vujčić, M. T., Demajo, M., Sabini, G., Cirrone, G. A. P.,& Cuttone, G.. (2004). Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays. in Oncology Reports, 12(6), 1323-1328.
https://hdl.handle.net/21.15107/rcub_vinar_2829

Ristić-Fira A, Petrović IM, Todorović DV, Korićanac L, Vujčić MT, Demajo M, Sabini G, Cirrone GAP, Cuttone G. Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays. in Oncology Reports. 2004;12(6):1323-1328.
https://hdl.handle.net/21.15107/rcub_vinar_2829 .

Ristić-Fira, Aleksandra, Petrović, Ivan M., Todorović, Danijela V., Korićanac, Lela, Vujčić, Miroslava T., Demajo, Miroslav, Sabini, G, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays" in Oncology Reports, 12, no. 6 (2004):1323-1328,
https://hdl.handle.net/21.15107/rcub_vinar_2829 .

TY  - CONF
AU  - Petrović, Ivan M.
AU  - Todorović, Danijela V.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9506
AB  - The aim of this study was to determine the initial inactivation of cells induced by high-energy proton beam designed for the treatment of eye melanoma. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam delivered over the single dose range from 8 Gy to 24 Gy. Position of samples was in the zone of the Bragg peak, having high LET values. Surviving fractions were evaluated at 6, 24 and 48 h post-irradiation. The survival curves exhibited a well-known shoulder, decreasing for doses higher than 8 Gy. Therefore, a significant dose dependent early cell inactivation after single delivery of 16 Gy to 24 Gy to the cell monolayer was observed. With the increase of the post-irradiation incubation time, a better killing effect, as the consequence of clonogenic survival, was detected.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells
VL  - 1
SP  - 426
EP  - 428
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9506
ER  - 

@conference{
author = "Petrović, Ivan M. and Todorović, Danijela V. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2004",
abstract = "The aim of this study was to determine the initial inactivation of cells induced by high-energy proton beam designed for the treatment of eye melanoma. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam delivered over the single dose range from 8 Gy to 24 Gy. Position of samples was in the zone of the Bragg peak, having high LET values. Surviving fractions were evaluated at 6, 24 and 48 h post-irradiation. The survival curves exhibited a well-known shoulder, decreasing for doses higher than 8 Gy. Therefore, a significant dose dependent early cell inactivation after single delivery of 16 Gy to 24 Gy to the cell monolayer was observed. With the increase of the post-irradiation incubation time, a better killing effect, as the consequence of clonogenic survival, was detected.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells",
volume = "1",
pages = "426-428",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9506"
}

Petrović, I. M., Todorović, D. V., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Ristić-Fira, A.. (2004). Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 426-428.
https://hdl.handle.net/21.15107/rcub_vinar_9506

Petrović IM, Todorović DV, Valastro LM, Cirrone GAP, Cuttone G, Ristić-Fira A. Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:426-428.
https://hdl.handle.net/21.15107/rcub_vinar_9506 .

Petrović, Ivan M., Todorović, Danijela V., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):426-428,
https://hdl.handle.net/21.15107/rcub_vinar_9506 .

TY  - CONF
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Valastro, Lucia M.
AU  - Petrović, Ivan M.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9507
AB  - The objective of this study was to determine whether apoptosis and cell cycle redistribution were influenced by high-LET irradiation. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam, within the Bragg peak, delivered over the single dose range from 8 Gy to 24 Gy. At 6 h post-irradiation, there was a low level of early apoptosis. At 48 h irradiated cells were more damaged, showing the increase in number of apoptotic nuclei. The dose dependent cell cycle phase distribution was detected at 48 h post-irradiation. The cell population exhibited phase redistribution toward G2/M phase.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells
VL  - 1
SP  - 429
EP  - 431
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9507
ER  - 

@conference{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Valastro, Lucia M. and Petrović, Ivan M.",
year = "2004",
abstract = "The objective of this study was to determine whether apoptosis and cell cycle redistribution were influenced by high-LET irradiation. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam, within the Bragg peak, delivered over the single dose range from 8 Gy to 24 Gy. At 6 h post-irradiation, there was a low level of early apoptosis. At 48 h irradiated cells were more damaged, showing the increase in number of apoptotic nuclei. The dose dependent cell cycle phase distribution was detected at 48 h post-irradiation. The cell population exhibited phase redistribution toward G2/M phase.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells",
volume = "1",
pages = "429-431",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9507"
}

Ristić-Fira, A., Todorović, D. V., Cirrone, G. A. P., Cuttone, G., Valastro, L. M.,& Petrović, I. M.. (2004). Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 429-431.
https://hdl.handle.net/21.15107/rcub_vinar_9507

Ristić-Fira A, Todorović DV, Cirrone GAP, Cuttone G, Valastro LM, Petrović IM. Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:429-431.
https://hdl.handle.net/21.15107/rcub_vinar_9507 .

Ristić-Fira, Aleksandra, Todorović, Danijela V., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Valastro, Lucia M., Petrović, Ivan M., "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):429-431,
https://hdl.handle.net/21.15107/rcub_vinar_9507 .

TY  - CONF
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Vujčić, Miroslava T.
AU  - Korićanac, Lela
AU  - Ruždijić, Sabera
AU  - Demajo, Miroslav
AU  - Cuttone, Giacomo
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8631
C3  - Turkish Journal of Biochemistry
T1  - Inactivation of melanoma cells irradiated with gamma rays and low energy protons
VL  - 28
IS  - 3
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8631
ER  - 

@conference{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Vujčić, Miroslava T. and Korićanac, Lela and Ruždijić, Sabera and Demajo, Miroslav and Cuttone, Giacomo",
year = "2003",
journal = "Turkish Journal of Biochemistry",
title = "Inactivation of melanoma cells irradiated with gamma rays and low energy protons",
volume = "28",
number = "3",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8631"
}

Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Vujčić, M. T., Korićanac, L., Ruždijić, S., Demajo, M.,& Cuttone, G.. (2003). Inactivation of melanoma cells irradiated with gamma rays and low energy protons. in Turkish Journal of Biochemistry, 28(3), 85.
https://hdl.handle.net/21.15107/rcub_vinar_8631

Petrović IM, Ristić-Fira A, Todorović DV, Vujčić MT, Korićanac L, Ruždijić S, Demajo M, Cuttone G. Inactivation of melanoma cells irradiated with gamma rays and low energy protons. in Turkish Journal of Biochemistry. 2003;28(3):85.
https://hdl.handle.net/21.15107/rcub_vinar_8631 .

Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Vujčić, Miroslava T., Korićanac, Lela, Ruždijić, Sabera, Demajo, Miroslav, Cuttone, Giacomo, "Inactivation of melanoma cells irradiated with gamma rays and low energy protons" in Turkish Journal of Biochemistry, 28, no. 3 (2003):85,
https://hdl.handle.net/21.15107/rcub_vinar_8631 .

TY  - JOUR
AU  - Ruždijić, Sabera
AU  - Milošević, Jovan
AU  - Popović, Nataša M.
AU  - Pešić, Milica
AU  - Stojilkovic, M
AU  - Kanazir, Selma
AU  - Todorović, Danijela V.
AU  - Ristić-Fira, Aleksandra
AU  - Krstić-Demonacos, Marija
AU  - Kanazir, Selma
AU  - Rakić, Ljubisav
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2423
AB  - Tiazofurin and 8-Cl-cAMP are novel antineoplastic agents that have been shown to be effective against various cancer cells in vitro and in vivo. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, apoptosis and downregulation of c-ras and c-myc gene expression. We examined the effects of 8-Cl-cAMP and tiazofurin, either separately or together, on apoptosis induction and c-fos and c-myc expression in melanoma cells. 8-Cl-cAMP and tiazofurin inhibited the growth of melanoma cells in a dose-responsive manner. Whether used separately or together, each agent induced apoptotic cell death. Apoptosis was accompanied by a marked inhibition of c-fos and c-mye gene expression. RT-PCR analysis showed that 8-Cl-cAMP, together with tiazofurin, promoted 61% and 75% decreases of c-myc and c-fos expression in melanoma cells respectively. These results clearly indicate that the combination of 8-Cl-cAMP and tiazofurin could provide a promising therapeutic approach for melanoma treatment.
T2  - Jugoslovenska Medicinska Biohemija
T1  - Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells
VL  - 20
IS  - 1
SP  - 9
EP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2423
ER  - 

@article{
author = "Ruždijić, Sabera and Milošević, Jovan and Popović, Nataša M. and Pešić, Milica and Stojilkovic, M and Kanazir, Selma and Todorović, Danijela V. and Ristić-Fira, Aleksandra and Krstić-Demonacos, Marija and Kanazir, Selma and Rakić, Ljubisav",
year = "2001",
abstract = "Tiazofurin and 8-Cl-cAMP are novel antineoplastic agents that have been shown to be effective against various cancer cells in vitro and in vivo. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, apoptosis and downregulation of c-ras and c-myc gene expression. We examined the effects of 8-Cl-cAMP and tiazofurin, either separately or together, on apoptosis induction and c-fos and c-myc expression in melanoma cells. 8-Cl-cAMP and tiazofurin inhibited the growth of melanoma cells in a dose-responsive manner. Whether used separately or together, each agent induced apoptotic cell death. Apoptosis was accompanied by a marked inhibition of c-fos and c-mye gene expression. RT-PCR analysis showed that 8-Cl-cAMP, together with tiazofurin, promoted 61% and 75% decreases of c-myc and c-fos expression in melanoma cells respectively. These results clearly indicate that the combination of 8-Cl-cAMP and tiazofurin could provide a promising therapeutic approach for melanoma treatment.",
journal = "Jugoslovenska Medicinska Biohemija",
title = "Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells",
volume = "20",
number = "1",
pages = "9-18",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2423"
}

Ruždijić, S., Milošević, J., Popović, N. M., Pešić, M., Stojilkovic, M., Kanazir, S., Todorović, D. V., Ristić-Fira, A., Krstić-Demonacos, M., Kanazir, S.,& Rakić, L.. (2001). Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells. in Jugoslovenska Medicinska Biohemija, 20(1), 9-18.
https://hdl.handle.net/21.15107/rcub_vinar_2423

Ruždijić S, Milošević J, Popović NM, Pešić M, Stojilkovic M, Kanazir S, Todorović DV, Ristić-Fira A, Krstić-Demonacos M, Kanazir S, Rakić L. Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells. in Jugoslovenska Medicinska Biohemija. 2001;20(1):9-18.
https://hdl.handle.net/21.15107/rcub_vinar_2423 .

Ruždijić, Sabera, Milošević, Jovan, Popović, Nataša M., Pešić, Milica, Stojilkovic, M, Kanazir, Selma, Todorović, Danijela V., Ristić-Fira, Aleksandra, Krstić-Demonacos, Marija, Kanazir, Selma, Rakić, Ljubisav, "Downregulation of c-fos and c-myc expression and apoptosis induction by tiazofurin and 8-Cl-cAMP in human melanoma cells" in Jugoslovenska Medicinska Biohemija, 20, no. 1 (2001):9-18,
https://hdl.handle.net/21.15107/rcub_vinar_2423 .

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Petrović, Ivan M.
AU  - Ruzvdijic, S
AU  - Raffaele, L
AU  - Sabini, MG
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Farruggia, G
AU  - Masotti, L
AU  - Kanazir, Dušan T.
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6321
AB  - The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.
T2  - Physica Medica
T1  - Inhibition of human melanoma cell growth by proton irradiation
VL  - 17
SP  - 71
EP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6321
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Petrović, Ivan M. and Ruzvdijic, S and Raffaele, L and Sabini, MG and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Farruggia, G and Masotti, L and Kanazir, Dušan T.",
year = "2001",
abstract = "The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.",
journal = "Physica Medica",
title = "Inhibition of human melanoma cell growth by proton irradiation",
volume = "17",
pages = "71-75",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6321"
}

Ristić-Fira, A., Todorović, D. V., Petrović, I. M., Ruzvdijic, S., Raffaele, L., Sabini, M., Cirrone, G. A. P., Cuttone, G., Farruggia, G., Masotti, L.,& Kanazir, D. T.. (2001). Inhibition of human melanoma cell growth by proton irradiation. in Physica Medica, 17, 71-75.
https://hdl.handle.net/21.15107/rcub_vinar_6321

Ristić-Fira A, Todorović DV, Petrović IM, Ruzvdijic S, Raffaele L, Sabini M, Cirrone GAP, Cuttone G, Farruggia G, Masotti L, Kanazir DT. Inhibition of human melanoma cell growth by proton irradiation. in Physica Medica. 2001;17:71-75.
https://hdl.handle.net/21.15107/rcub_vinar_6321 .

Ristić-Fira, Aleksandra, Todorović, Danijela V., Petrović, Ivan M., Ruzvdijic, S, Raffaele, L, Sabini, MG, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Farruggia, G, Masotti, L, Kanazir, Dušan T., "Inhibition of human melanoma cell growth by proton irradiation" in Physica Medica, 17 (2001):71-75,
https://hdl.handle.net/21.15107/rcub_vinar_6321 .