TY  - JOUR
AU  - Kolesnikova, I. A.
AU  - Lalkovičova, M.
AU  - Severyukhin, Yu. S.
AU  - Golikova, K. N.
AU  - Utina, D. M.
AU  - Pronskikh, E. V.
AU  - Despotović, Sanja Z.
AU  - Gaevsky, V. N.
AU  - Pirić, David
AU  - Masnikosa, Romana
AU  - Budennaya, N. N.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11224
AB  - We designed a study with the objective to determine the long-term radiation effects of gamma rays, originating from a single shot of Co60 at a dose of 2 Gy on the 7-month-old male mice of the ICR line in 30 days after the irradiation. The aim of this study was to characterize the behavior of animals using the Open Field test, immuno-hematological status, and morpho-functional changes in the central nervous system of mice. Irradiated animals displayed significantly different behavior in the OF in comparison with the control group. The radiation damage was confirmed by assessing the ratio of leukocytes in the peripheral blood of mice at a later date after exposure to Co60. After irradiation, a decrease in the glioneuronal complex was observed in the irritated group as well as histological changes of brain cells. To sum up, not only was the hematological status of mice altered upon the total gamma irradiation, but also their behavior, which was most probably due to significant alterations in the CNS. Graphical Abstract: Study of influence of ionizing radiation on female mice, comparison between different age groups. Open Field test on the 30 days after 2 Gy of γ-rays and histological analysis indicated changes in behavioral patterns, leucocytes, and brain tissue. [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Cellular and Molecular Neurobiology
T1  - The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes
VL  - InPress
DO  - 10.1007/s10571-023-01381-1
ER  - 

@article{
author = "Kolesnikova, I. A. and Lalkovičova, M. and Severyukhin, Yu. S. and Golikova, K. N. and Utina, D. M. and Pronskikh, E. V. and Despotović, Sanja Z. and Gaevsky, V. N. and Pirić, David and Masnikosa, Romana and Budennaya, N. N.",
year = "2023",
abstract = "We designed a study with the objective to determine the long-term radiation effects of gamma rays, originating from a single shot of Co60 at a dose of 2 Gy on the 7-month-old male mice of the ICR line in 30 days after the irradiation. The aim of this study was to characterize the behavior of animals using the Open Field test, immuno-hematological status, and morpho-functional changes in the central nervous system of mice. Irradiated animals displayed significantly different behavior in the OF in comparison with the control group. The radiation damage was confirmed by assessing the ratio of leukocytes in the peripheral blood of mice at a later date after exposure to Co60. After irradiation, a decrease in the glioneuronal complex was observed in the irritated group as well as histological changes of brain cells. To sum up, not only was the hematological status of mice altered upon the total gamma irradiation, but also their behavior, which was most probably due to significant alterations in the CNS. Graphical Abstract: Study of influence of ionizing radiation on female mice, comparison between different age groups. Open Field test on the 30 days after 2 Gy of γ-rays and histological analysis indicated changes in behavioral patterns, leucocytes, and brain tissue. [Figure not available: see fulltext.] © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Cellular and Molecular Neurobiology",
title = "The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes",
volume = "InPress",
doi = "10.1007/s10571-023-01381-1"
}

Kolesnikova, I. A., Lalkovičova, M., Severyukhin, Yu. S., Golikova, K. N., Utina, D. M., Pronskikh, E. V., Despotović, S. Z., Gaevsky, V. N., Pirić, D., Masnikosa, R.,& Budennaya, N. N.. (2023). The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes. in Cellular and Molecular Neurobiology, InPress.
https://doi.org/10.1007/s10571-023-01381-1

Kolesnikova IA, Lalkovičova M, Severyukhin YS, Golikova KN, Utina DM, Pronskikh EV, Despotović SZ, Gaevsky VN, Pirić D, Masnikosa R, Budennaya NN. The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes. in Cellular and Molecular Neurobiology. 2023;InPress.
doi:10.1007/s10571-023-01381-1 .

Kolesnikova, I. A., Lalkovičova, M., Severyukhin, Yu. S., Golikova, K. N., Utina, D. M., Pronskikh, E. V., Despotović, Sanja Z., Gaevsky, V. N., Pirić, David, Masnikosa, Romana, Budennaya, N. N., "The Effects of Whole Body Gamma Irradiation on Mice, Age-Related Behavioral, and Pathophysiological Changes" in Cellular and Molecular Neurobiology, InPress (2023),
https://doi.org/10.1007/s10571-023-01381-1 . .

TY  - JOUR
AU  - Despotović, Sanja Z.
AU  - Ćosić, Marko
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10381
AB  - The morphological method—based on the topology and singularity theory and originally developed for the analysis of the scattering experiments—was extended to be applicable for the analysis of biological data. The usefulness of the topological viewpoint was demonstrated by quantification of the changes in collagen fiber straightness in the human colon mucosa (healthy mucosa, colorectal cancer, and uninvolved mucosa far from cancer). This has been done by modeling the distribution of collagen segment angles by the polymorphic beta-distribution. Its shapes were classified according to the number and type of critical points. We found that biologically relevant shapes could be classified as shapes without any preferable orientation (i.e. shapes without local extrema), transitional forms (i.e. forms with one broad local maximum), and highly oriented forms (i.e. forms with two minima at both ends and one very narrow maximum between them). Thus, changes in the fiber organization were linked to the metamorphoses of the beta-distribution forms. The obtained classification was used to define a new, shape-aware/based, measure of the collagen straightness, which revealed a slight and moderate increase of the straightness in mucosa samples taken 20 and 10 cm away from the tumor. The largest increase of collagen straightness was found in samples of cancer tissue. Samples of healthy individuals have a uniform distribution of beta-distribution forms. We found that this distribution has the maximal information entropy. At 20 cm and 10 cm away from cancer, the transition forms redistribute into unoriented and highly oriented forms. Closer to cancer the number of unoriented forms decreases rapidly leaving only highly oriented forms present in the samples of the cancer tissue, whose distribution has minimal information entropy. The polarization of the distribution was followed by a significant increase in the number of quasi-symmetrical forms in samples 20 cm away from cancer which decreases closer to cancer. This work shows that the evolution of the distribution of the beta-distribution forms—an abstract construction of the mind—follows the familiar laws of statistical mechanics. Additionally, the polarization of the beta-distribution forms together with the described change in the number of quasi-symmetrical forms, clearly visible in the parametric space of the beta-distribution and very difficult to notice in the observable space, can be a useful indicator of the early stages in the development of colorectal cancer.
T2  - Frontiers in Physics
T1  - The Morphological Analysis of the Collagen Fiber Straightness in the Healthy Uninvolved Human Colon Mucosa Away From the Cancer
VL  - 10
DO  - 10.3389/fphy.2022.915644
ER  - 

@article{
author = "Despotović, Sanja Z. and Ćosić, Marko",
year = "2022",
abstract = "The morphological method—based on the topology and singularity theory and originally developed for the analysis of the scattering experiments—was extended to be applicable for the analysis of biological data. The usefulness of the topological viewpoint was demonstrated by quantification of the changes in collagen fiber straightness in the human colon mucosa (healthy mucosa, colorectal cancer, and uninvolved mucosa far from cancer). This has been done by modeling the distribution of collagen segment angles by the polymorphic beta-distribution. Its shapes were classified according to the number and type of critical points. We found that biologically relevant shapes could be classified as shapes without any preferable orientation (i.e. shapes without local extrema), transitional forms (i.e. forms with one broad local maximum), and highly oriented forms (i.e. forms with two minima at both ends and one very narrow maximum between them). Thus, changes in the fiber organization were linked to the metamorphoses of the beta-distribution forms. The obtained classification was used to define a new, shape-aware/based, measure of the collagen straightness, which revealed a slight and moderate increase of the straightness in mucosa samples taken 20 and 10 cm away from the tumor. The largest increase of collagen straightness was found in samples of cancer tissue. Samples of healthy individuals have a uniform distribution of beta-distribution forms. We found that this distribution has the maximal information entropy. At 20 cm and 10 cm away from cancer, the transition forms redistribute into unoriented and highly oriented forms. Closer to cancer the number of unoriented forms decreases rapidly leaving only highly oriented forms present in the samples of the cancer tissue, whose distribution has minimal information entropy. The polarization of the distribution was followed by a significant increase in the number of quasi-symmetrical forms in samples 20 cm away from cancer which decreases closer to cancer. This work shows that the evolution of the distribution of the beta-distribution forms—an abstract construction of the mind—follows the familiar laws of statistical mechanics. Additionally, the polarization of the beta-distribution forms together with the described change in the number of quasi-symmetrical forms, clearly visible in the parametric space of the beta-distribution and very difficult to notice in the observable space, can be a useful indicator of the early stages in the development of colorectal cancer.",
journal = "Frontiers in Physics",
title = "The Morphological Analysis of the Collagen Fiber Straightness in the Healthy Uninvolved Human Colon Mucosa Away From the Cancer",
volume = "10",
doi = "10.3389/fphy.2022.915644"
}

Despotović, S. Z.,& Ćosić, M.. (2022). The Morphological Analysis of the Collagen Fiber Straightness in the Healthy Uninvolved Human Colon Mucosa Away From the Cancer. in Frontiers in Physics, 10.
https://doi.org/10.3389/fphy.2022.915644

Despotović SZ, Ćosić M. The Morphological Analysis of the Collagen Fiber Straightness in the Healthy Uninvolved Human Colon Mucosa Away From the Cancer. in Frontiers in Physics. 2022;10.
doi:10.3389/fphy.2022.915644 .

Despotović, Sanja Z., Ćosić, Marko, "The Morphological Analysis of the Collagen Fiber Straightness in the Healthy Uninvolved Human Colon Mucosa Away From the Cancer" in Frontiers in Physics, 10 (2022),
https://doi.org/10.3389/fphy.2022.915644 . .