Petrović, Sandra

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Authority KeyName Variants
orcid::0000-0003-0930-6455
  • Petrović, Sandra (43)
Projects
Studies of enzyme interactions with toxic and pharmacologically active molecules Radiosensitivity of human genome
[1956] Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) Ministry of Science and Environmental Protection of Serbia [143046]
AIRC [IG-12085], Beneficentia Stiftung Vaduz Beneficentia Stiftung (Vaduz), ITT (Istituto Toscano Tumori), Fondazione Cassa Risparmio Firenze (CRF), AIRC [IG-16049], AIRC-FIRC (Fondazione Italiana per la Ricerca sul Cancro) [18044]
CMST COST Action [CM1203 (PoCheMoN)] COST action CM1203 Polyoxometalate Chemistry for Molecular Nanoscience (PoCheMoN), COST-STSM-ECOST-STSM-CM1203-030416-072554
COST Action [MP1302] Fanconi Anemia Research Fund
FIRB-MERIT [RBNE08HWLZ_012] Hydrogen Energy - Research and Development of New Materials: Electrolytic Hydrogen Production, Hydrogen Fuel Cells, Isotope Effects
Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites Microbial diversity study and characterization of beneficial environmental microorganisms
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200178 (University of Belgrade, Faculty of Biology) Application of low temperature plasmas in biomedicine, environmental protection and nanotechnologies
Molekularni mehanizmi radiosenzitivnosti humanog genoma Application of biotechnological methods for sustainable exploitation of by-products of agro-industry
Ministry of Science and Technological Development of the Republic of Serbia [142051], Ministry of Higher Education, Science and Technology of the Republic of Slovenia Ministry of Science and Technological Development, Republic of Serbia [142001, 143046]
NATO SfP [984555] Serbian Ministry of Science and Environmental Protection [143046]
Serbian Ministry of Science and Technological Development [143046]

Author's Bibliography

Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro

Ivković, Ivana; Bukvički, Danka; Novaković, Miroslav M.; Majstorović, Ivana; Leskovac, Andreja; Petrović, Sandra; Veljić, Milan

(2021)

TY  - JOUR
AU  - Ivković, Ivana
AU  - Bukvički, Danka
AU  - Novaković, Miroslav M.
AU  - Majstorović, Ivana
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Veljić, Milan
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10051
AB  - Liverworts are characterized by a high content of bioactive compounds reported to show antimicrobial, anticancer, and antioxidant properties. In this study, the biological effects of the methanol extract of the liverwort Pellia endiviifolia and its constituents, bis-bibenzyls perrottetin E, 10′-hydroxyperrottetin E, and 10,10′-dihydroxyperrottetin E, were investigated using human peripheral blood cells as a model system. The assessment of the investigated compounds comprised testing their genotoxicity, apoptotic potential, and redox modulating activities. The genotoxicity testing indicated that medium (25 µM) and high concentrations (100 µM) of the investigated compounds displayed genotoxic and antiproliferative effects in human lymphocytes as revealed by significant, concentration-dependent enhancement of the micronuclei incidence and decrease in the cytokinesis-block proliferation index compared to the control (P <.001). Analysis of leukocyte apoptosis showed a substantial potential of all investigated compounds to induce apoptosis, which was not concentration-dependent. The P endiviifolia extract and perrottetin E demonstrated considerable pro-apoptotic potential, even at the lowest concentration (1 µM) applied. Evaluation of the redox modulating effects, which comprised measuring erythrocyte catalase activity and the lymphocyte malondialdehyde level, showed that the investigated compounds did not induce oxidative stress in human peripheral blood cells (P >.05). The observed genotoxic, antiproliferative, and proapoptotic effects of the investigated compounds make them suitable for further comprehensive studies related to their possible applications as anticancer agents.
T2  - Natural Product Communications
T1  - Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro
VL  - 16
IS  - 11
SP  - 1
DO  - 10.1177/1934578X211056422
ER  - 
@article{
author = "Ivković, Ivana and Bukvički, Danka and Novaković, Miroslav M. and Majstorović, Ivana and Leskovac, Andreja and Petrović, Sandra and Veljić, Milan",
year = "2021",
abstract = "Liverworts are characterized by a high content of bioactive compounds reported to show antimicrobial, anticancer, and antioxidant properties. In this study, the biological effects of the methanol extract of the liverwort Pellia endiviifolia and its constituents, bis-bibenzyls perrottetin E, 10′-hydroxyperrottetin E, and 10,10′-dihydroxyperrottetin E, were investigated using human peripheral blood cells as a model system. The assessment of the investigated compounds comprised testing their genotoxicity, apoptotic potential, and redox modulating activities. The genotoxicity testing indicated that medium (25 µM) and high concentrations (100 µM) of the investigated compounds displayed genotoxic and antiproliferative effects in human lymphocytes as revealed by significant, concentration-dependent enhancement of the micronuclei incidence and decrease in the cytokinesis-block proliferation index compared to the control (P <.001). Analysis of leukocyte apoptosis showed a substantial potential of all investigated compounds to induce apoptosis, which was not concentration-dependent. The P endiviifolia extract and perrottetin E demonstrated considerable pro-apoptotic potential, even at the lowest concentration (1 µM) applied. Evaluation of the redox modulating effects, which comprised measuring erythrocyte catalase activity and the lymphocyte malondialdehyde level, showed that the investigated compounds did not induce oxidative stress in human peripheral blood cells (P >.05). The observed genotoxic, antiproliferative, and proapoptotic effects of the investigated compounds make them suitable for further comprehensive studies related to their possible applications as anticancer agents.",
journal = "Natural Product Communications",
title = "Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro",
volume = "16",
number = "11",
pages = "1",
doi = "10.1177/1934578X211056422"
}
Ivković, I., Bukvički, D., Novaković, M. M., Majstorović, I., Leskovac, A., Petrović, S.,& Veljić, M.. (2021). Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro. in Natural Product Communications, 16(11), 1.
https://doi.org/10.1177/1934578X211056422
Ivković I, Bukvički D, Novaković MM, Majstorović I, Leskovac A, Petrović S, Veljić M. Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro. in Natural Product Communications. 2021;16(11):1.
doi:10.1177/1934578X211056422 .
Ivković, Ivana, Bukvički, Danka, Novaković, Miroslav M., Majstorović, Ivana, Leskovac, Andreja, Petrović, Sandra, Veljić, Milan, "Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro" in Natural Product Communications, 16, no. 11 (2021):1,
https://doi.org/10.1177/1934578X211056422 . .

Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles

Stamenović, Una; Davidović, Slađana; Petrović, Sandra; Leskovac, Andreja; Stoiljković, Milovan; Vodnik, Vesna

(2021)

TY  - JOUR
AU  - Stamenović, Una
AU  - Davidović, Slađana
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Stoiljković, Milovan
AU  - Vodnik, Vesna
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9874
AB  - Two silver–polyaniline/polyvinylpyrrolidone (Ag–PANI/PVP) nanocomposites were prepared using in situ integration of silver nanoparticles (AgNPs) during oxidative aniline polymerization, accelerated by the presence of PVP, which as well minimized the risk of particle agglomeration and macroscopic precipitation. Both nanocomposites have similar silver content (∼44 wt% Ag) but different morphological features of AgNPs (spheres/triangles) and polymers (granular/wrinkling pattern). Several spectroscopic, macroscopic, and analytical techniques, microscopy, and surface analysis methods have been used to analyze their physical and chemical properties. Investigation of their antimicrobial potential and possible application as an effective weapon against indicator microbial strains, E. coli, S. aureus, and C. albicans, has shown inhibition of microbial growth by more than 90%, even at low composite concentrations (5 ppm) and short contact time (4 h). A biosafety assessment of Ag–PANI/PVP that comprised testing the genotoxicity and redox modulating activity was performed using human peripheral blood cells as a model system. The obtained results have shown that the investigated Ag–PANI/PVP exhibited significant prooxidant and cytostatic effects (p < 0.05) with no apparent potential to induce DNA damage. Although precautions should be taken to protect human health, the significant antimicrobial efficiency of Ag–PANI/PVP makes it suitable for further studies and applications in non-medical areas, such as wastewater treatment.
T2  - New Journal of Chemistry
T1  - Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles
VL  - 45
IS  - 28
SP  - 12711
EP  - 12720
DO  - 10.1039/D1NJ02729H
ER  - 
@article{
author = "Stamenović, Una and Davidović, Slađana and Petrović, Sandra and Leskovac, Andreja and Stoiljković, Milovan and Vodnik, Vesna",
year = "2021",
abstract = "Two silver–polyaniline/polyvinylpyrrolidone (Ag–PANI/PVP) nanocomposites were prepared using in situ integration of silver nanoparticles (AgNPs) during oxidative aniline polymerization, accelerated by the presence of PVP, which as well minimized the risk of particle agglomeration and macroscopic precipitation. Both nanocomposites have similar silver content (∼44 wt% Ag) but different morphological features of AgNPs (spheres/triangles) and polymers (granular/wrinkling pattern). Several spectroscopic, macroscopic, and analytical techniques, microscopy, and surface analysis methods have been used to analyze their physical and chemical properties. Investigation of their antimicrobial potential and possible application as an effective weapon against indicator microbial strains, E. coli, S. aureus, and C. albicans, has shown inhibition of microbial growth by more than 90%, even at low composite concentrations (5 ppm) and short contact time (4 h). A biosafety assessment of Ag–PANI/PVP that comprised testing the genotoxicity and redox modulating activity was performed using human peripheral blood cells as a model system. The obtained results have shown that the investigated Ag–PANI/PVP exhibited significant prooxidant and cytostatic effects (p < 0.05) with no apparent potential to induce DNA damage. Although precautions should be taken to protect human health, the significant antimicrobial efficiency of Ag–PANI/PVP makes it suitable for further studies and applications in non-medical areas, such as wastewater treatment.",
journal = "New Journal of Chemistry",
title = "Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles",
volume = "45",
number = "28",
pages = "12711-12720",
doi = "10.1039/D1NJ02729H"
}
Stamenović, U., Davidović, S., Petrović, S., Leskovac, A., Stoiljković, M.,& Vodnik, V.. (2021). Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles. in New Journal of Chemistry, 45(28), 12711-12720.
https://doi.org/10.1039/D1NJ02729H
Stamenović U, Davidović S, Petrović S, Leskovac A, Stoiljković M, Vodnik V. Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles. in New Journal of Chemistry. 2021;45(28):12711-12720.
doi:10.1039/D1NJ02729H .
Stamenović, Una, Davidović, Slađana, Petrović, Sandra, Leskovac, Andreja, Stoiljković, Milovan, Vodnik, Vesna, "Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles" in New Journal of Chemistry, 45, no. 28 (2021):12711-12720,
https://doi.org/10.1039/D1NJ02729H . .

A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition

Bondžić, Aleksandra M.; Lazarević-Pašti, Tamara; Leskovac, Andreja; Petrović, Sandra; Čolović, Mirjana B.; Parac Vogt, Tatjana N.; Janjić, Goran V.

(2020)

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Lazarević-Pašti, Tamara
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Čolović, Mirjana B.
AU  - Parac Vogt, Tatjana N.
AU  - Janjić, Goran V.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9028
AB  - Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors,12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate(POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism andtryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significantchanges in the secondary structure of the enzyme. The molecular docking approach has revealed a newallosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChEby POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is consideredresponsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selectedPOMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. Itwas shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, whichindicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable ofbinding to an allosteric site that so far has not been considered responsible for enzyme inhibition could befundamental for the development of new drug design strategies and the discovery of more efficient AChEmodulators.
T2  - European Journal of Pharmaceutical Sciences
T1  - A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition
VL  - 151
SP  - 105376
DO  - 10.1016/j.ejps.2020.105376
ER  - 
@article{
author = "Bondžić, Aleksandra M. and Lazarević-Pašti, Tamara and Leskovac, Andreja and Petrović, Sandra and Čolović, Mirjana B. and Parac Vogt, Tatjana N. and Janjić, Goran V.",
year = "2020",
abstract = "Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors,12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate(POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism andtryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significantchanges in the secondary structure of the enzyme. The molecular docking approach has revealed a newallosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChEby POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is consideredresponsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selectedPOMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. Itwas shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, whichindicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable ofbinding to an allosteric site that so far has not been considered responsible for enzyme inhibition could befundamental for the development of new drug design strategies and the discovery of more efficient AChEmodulators.",
journal = "European Journal of Pharmaceutical Sciences",
title = "A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition",
volume = "151",
pages = "105376",
doi = "10.1016/j.ejps.2020.105376"
}
Bondžić, A. M., Lazarević-Pašti, T., Leskovac, A., Petrović, S., Čolović, M. B., Parac Vogt, T. N.,& Janjić, G. V.. (2020). A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition. in European Journal of Pharmaceutical Sciences, 151, 105376.
https://doi.org/10.1016/j.ejps.2020.105376
Bondžić AM, Lazarević-Pašti T, Leskovac A, Petrović S, Čolović MB, Parac Vogt TN, Janjić GV. A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition. in European Journal of Pharmaceutical Sciences. 2020;151:105376.
doi:10.1016/j.ejps.2020.105376 .
Bondžić, Aleksandra M., Lazarević-Pašti, Tamara, Leskovac, Andreja, Petrović, Sandra, Čolović, Mirjana B., Parac Vogt, Tatjana N., Janjić, Goran V., "A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition" in European Journal of Pharmaceutical Sciences, 151 (2020):105376,
https://doi.org/10.1016/j.ejps.2020.105376 . .
8
6

Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles

Laban, Bojana B.; Ralević, Uroš; Petrović, Sandra; Leskovac, Andreja; Vasić Anićijević, Dragana D.; Marković, Mirjana; Vasić, Vesna M.

(2020)

TY  - JOUR
AU  - Laban, Bojana B.
AU  - Ralević, Uroš
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Vasić Anićijević, Dragana D.
AU  - Marković, Mirjana
AU  - Vasić, Vesna M.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8673
AB  - The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019
T2  - Journal of Inorganic Biochemistry
T1  - Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles
VL  - 204
SP  - 110958
DO  - 10.1016/j.jinorgbio.2019.110958
ER  - 
@article{
author = "Laban, Bojana B. and Ralević, Uroš and Petrović, Sandra and Leskovac, Andreja and Vasić Anićijević, Dragana D. and Marković, Mirjana and Vasić, Vesna M.",
year = "2020",
abstract = "The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019",
journal = "Journal of Inorganic Biochemistry",
title = "Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles",
volume = "204",
pages = "110958",
doi = "10.1016/j.jinorgbio.2019.110958"
}
Laban, B. B., Ralević, U., Petrović, S., Leskovac, A., Vasić Anićijević, D. D., Marković, M.,& Vasić, V. M.. (2020). Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles. in Journal of Inorganic Biochemistry, 204, 110958.
https://doi.org/10.1016/j.jinorgbio.2019.110958
Laban BB, Ralević U, Petrović S, Leskovac A, Vasić Anićijević DD, Marković M, Vasić VM. Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles. in Journal of Inorganic Biochemistry. 2020;204:110958.
doi:10.1016/j.jinorgbio.2019.110958 .
Laban, Bojana B., Ralević, Uroš, Petrović, Sandra, Leskovac, Andreja, Vasić Anićijević, Dragana D., Marković, Mirjana, Vasić, Vesna M., "Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles" in Journal of Inorganic Biochemistry, 204 (2020):110958,
https://doi.org/10.1016/j.jinorgbio.2019.110958 . .
14
10
12

Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue

Bondžić, Aleksandra M.; Leskovac, Andreja; Petrović, Sandra; Vasić Anićijević, Dragana D.; Luce, Marco; Massai, Lara; Generosi, Amanda; Paci, Barbara; Cricenti, Antonio; Messori, Luigi; Vasić, Vesna M.

(2019)

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Vasić Anićijević, Dragana D.
AU  - Luce, Marco
AU  - Massai, Lara
AU  - Generosi, Amanda
AU  - Paci, Barbara
AU  - Cricenti, Antonio
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8747
AB  - Citrate-capped gold nanoparticles (AuNPs) were functionalized with three distinct antitumor gold(III) complexes, e.g., [Au(N,N)(OH)2][PF6], where (N,N)=2,2′-bipyridine; [Au(C,N)(AcO)2], where (C,N)=deprotonated 6-(1,1-dimethylbenzyl)-pyridine; [Au(C,N,N)(OH)][PF6], where (C,N,N)=deprotonated 6-(1,1-dimethylbenzyl)-2,2′-bipyridine, to assess the chance of tracking their subcellular distribution by atomic force microscopy (AFM), and surface enhanced Raman spectroscopy (SERS) techniques. An extensive physicochemical characterization of the formed conjugates was, thus, carried out by applying a variety of methods (density functional theory—DFT, UV/Vis spectrophotometry, AFM, Raman spectroscopy, and SERS). The resulting gold(III) complexes/AuNPs conjugates turned out to be pretty stable. Interestingly, they exhibited a dramatically increased resonance intensity in the Raman spectra induced by AuNPs. For testing the use of the functionalized AuNPs for biosensing, their distribution in the nuclear, cytosolic, and membrane cell fractions obtained from human lymphocytes was investigated by AFM and SERS. The conjugates were detected in the membrane and nuclear cell fractions but not in the cytosol. The AFM method confirmed that conjugates induced changes in the morphology and nanostructure of the membrane and nuclear fractions. The obtained results point out that the conjugates formed between AuNPs and gold(III) complexes may be used as a tool for tracking metallodrug distribution in the different cell fractions.
T2  - International Journal of Molecular Sciences
T1  - Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue
VL  - 20
IS  - 24
SP  - 6306
DO  - 10.3390/ijms20246306
ER  - 
@article{
author = "Bondžić, Aleksandra M. and Leskovac, Andreja and Petrović, Sandra and Vasić Anićijević, Dragana D. and Luce, Marco and Massai, Lara and Generosi, Amanda and Paci, Barbara and Cricenti, Antonio and Messori, Luigi and Vasić, Vesna M.",
year = "2019",
abstract = "Citrate-capped gold nanoparticles (AuNPs) were functionalized with three distinct antitumor gold(III) complexes, e.g., [Au(N,N)(OH)2][PF6], where (N,N)=2,2′-bipyridine; [Au(C,N)(AcO)2], where (C,N)=deprotonated 6-(1,1-dimethylbenzyl)-pyridine; [Au(C,N,N)(OH)][PF6], where (C,N,N)=deprotonated 6-(1,1-dimethylbenzyl)-2,2′-bipyridine, to assess the chance of tracking their subcellular distribution by atomic force microscopy (AFM), and surface enhanced Raman spectroscopy (SERS) techniques. An extensive physicochemical characterization of the formed conjugates was, thus, carried out by applying a variety of methods (density functional theory—DFT, UV/Vis spectrophotometry, AFM, Raman spectroscopy, and SERS). The resulting gold(III) complexes/AuNPs conjugates turned out to be pretty stable. Interestingly, they exhibited a dramatically increased resonance intensity in the Raman spectra induced by AuNPs. For testing the use of the functionalized AuNPs for biosensing, their distribution in the nuclear, cytosolic, and membrane cell fractions obtained from human lymphocytes was investigated by AFM and SERS. The conjugates were detected in the membrane and nuclear cell fractions but not in the cytosol. The AFM method confirmed that conjugates induced changes in the morphology and nanostructure of the membrane and nuclear fractions. The obtained results point out that the conjugates formed between AuNPs and gold(III) complexes may be used as a tool for tracking metallodrug distribution in the different cell fractions.",
journal = "International Journal of Molecular Sciences",
title = "Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue",
volume = "20",
number = "24",
pages = "6306",
doi = "10.3390/ijms20246306"
}
Bondžić, A. M., Leskovac, A., Petrović, S., Vasić Anićijević, D. D., Luce, M., Massai, L., Generosi, A., Paci, B., Cricenti, A., Messori, L.,& Vasić, V. M.. (2019). Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue. in International Journal of Molecular Sciences, 20(24), 6306.
https://doi.org/10.3390/ijms20246306
Bondžić AM, Leskovac A, Petrović S, Vasić Anićijević DD, Luce M, Massai L, Generosi A, Paci B, Cricenti A, Messori L, Vasić VM. Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue. in International Journal of Molecular Sciences. 2019;20(24):6306.
doi:10.3390/ijms20246306 .
Bondžić, Aleksandra M., Leskovac, Andreja, Petrović, Sandra, Vasić Anićijević, Dragana D., Luce, Marco, Massai, Lara, Generosi, Amanda, Paci, Barbara, Cricenti, Antonio, Messori, Luigi, Vasić, Vesna M., "Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue" in International Journal of Molecular Sciences, 20, no. 24 (2019):6306,
https://doi.org/10.3390/ijms20246306 . .
3
1
3

UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations

Savić, Jasmina; Petrović, Sandra; Leskovac, Andreja; Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Tanović, Brankica B.; Gašić, Slavica M.; Vasić, Vesna M.

(2019)

TY  - JOUR
AU  - Savić, Jasmina
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Tanović, Brankica B.
AU  - Gašić, Slavica M.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7821
AB  - UV-C irradiation is widely used in the food industry. However, the health effects from dietary exposure to the irradiated pesticide residues retained in foodstuffs are underestimated. In this study, technical chlorpyrifos (TCPF) and its oil in water (EW) and emulsifiable concentrate (EC) formulations were irradiated by UV-C, and their photodegradation products were subjected to toxicity assessment, including determination of acetylcholinesterase (AChE) activity, genotoxicity and oxidative stress using human blood cells as a model system. Toxicity studies were performed using the chlorpyrifos concentrations in the range of those proposed as the maximum residue levels in plant commodities. TCPF, EW and EC photodegradation products induced DNA damage and oxidative stress, and their genotoxicity did not decrease as a function of irradiation time. Irradiated TCPF and EC are more potent AChE inhibitors than irradiated EW. Accordingly, the application of UV-C irradiation must be considered when processing the plants previously treated with chlorpyrifos formulations. © 2018 Elsevier Ltd
T2  - Food Chemistry
T1  - UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations
VL  - 271
SP  - 469
EP  - 478
DO  - 10.1016/j.foodchem.2018.07.207
ER  - 
@article{
author = "Savić, Jasmina and Petrović, Sandra and Leskovac, Andreja and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Tanović, Brankica B. and Gašić, Slavica M. and Vasić, Vesna M.",
year = "2019",
abstract = "UV-C irradiation is widely used in the food industry. However, the health effects from dietary exposure to the irradiated pesticide residues retained in foodstuffs are underestimated. In this study, technical chlorpyrifos (TCPF) and its oil in water (EW) and emulsifiable concentrate (EC) formulations were irradiated by UV-C, and their photodegradation products were subjected to toxicity assessment, including determination of acetylcholinesterase (AChE) activity, genotoxicity and oxidative stress using human blood cells as a model system. Toxicity studies were performed using the chlorpyrifos concentrations in the range of those proposed as the maximum residue levels in plant commodities. TCPF, EW and EC photodegradation products induced DNA damage and oxidative stress, and their genotoxicity did not decrease as a function of irradiation time. Irradiated TCPF and EC are more potent AChE inhibitors than irradiated EW. Accordingly, the application of UV-C irradiation must be considered when processing the plants previously treated with chlorpyrifos formulations. © 2018 Elsevier Ltd",
journal = "Food Chemistry",
title = "UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations",
volume = "271",
pages = "469-478",
doi = "10.1016/j.foodchem.2018.07.207"
}
Savić, J., Petrović, S., Leskovac, A., Lazarević-Pašti, T., Nastasijević, B. J., Tanović, B. B., Gašić, S. M.,& Vasić, V. M.. (2019). UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry, 271, 469-478.
https://doi.org/10.1016/j.foodchem.2018.07.207
Savić J, Petrović S, Leskovac A, Lazarević-Pašti T, Nastasijević BJ, Tanović BB, Gašić SM, Vasić VM. UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry. 2019;271:469-478.
doi:10.1016/j.foodchem.2018.07.207 .
Savić, Jasmina, Petrović, Sandra, Leskovac, Andreja, Lazarević-Pašti, Tamara, Nastasijević, Branislav J., Tanović, Brankica B., Gašić, Slavica M., Vasić, Vesna M., "UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations" in Food Chemistry, 271 (2019):469-478,
https://doi.org/10.1016/j.foodchem.2018.07.207 . .
11
8

Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations

Savić, Jasmina; Petrović, Sandra; Leskovac, Andreja; Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Tanović, Brankica B.; Gašić, Slavica M.; Vasić, Vesna M.

(2019)

TY  - GEN
AU  - Savić, Jasmina
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Tanović, Brankica B.
AU  - Gašić, Slavica M.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7831
AB  - Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; 
Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; 
Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4
T2  - Food Chemistry
T1  - Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations
VL  - 271
SP  - 469
EP  - 478
DO  - 10.1016/j.foodchem.2018.07.207
ER  - 
@misc{
author = "Savić, Jasmina and Petrović, Sandra and Leskovac, Andreja and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Tanović, Brankica B. and Gašić, Slavica M. and Vasić, Vesna M.",
year = "2019",
abstract = "Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; 
Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; 
Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4",
journal = "Food Chemistry",
title = "Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations",
volume = "271",
pages = "469-478",
doi = "10.1016/j.foodchem.2018.07.207"
}
Savić, J., Petrović, S., Leskovac, A., Lazarević-Pašti, T., Nastasijević, B. J., Tanović, B. B., Gašić, S. M.,& Vasić, V. M.. (2019). Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry, 271, 469-478.
https://doi.org/10.1016/j.foodchem.2018.07.207
Savić J, Petrović S, Leskovac A, Lazarević-Pašti T, Nastasijević BJ, Tanović BB, Gašić SM, Vasić VM. Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry. 2019;271:469-478.
doi:10.1016/j.foodchem.2018.07.207 .
Savić, Jasmina, Petrović, Sandra, Leskovac, Andreja, Lazarević-Pašti, Tamara, Nastasijević, Branislav J., Tanović, Brankica B., Gašić, Slavica M., Vasić, Vesna M., "Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations" in Food Chemistry, 271 (2019):469-478,
https://doi.org/10.1016/j.foodchem.2018.07.207 . .
11
8

Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents

Leskovac, Andreja; Petrović, Sandra; Lazarević-Pašti, Tamara; Krstić, Milena P.; Vasić, Vesna M.

(2018)

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Lazarević-Pašti, Tamara
AU  - Krstić, Milena P.
AU  - Vasić, Vesna M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s00775-018-1560-x
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7765
AB  - In recent years, the search for effective anticancer compounds based on transition metal complexes has been the focus of medical investigations. The synergy between the ruthenium(II) and N-alkylphenothiazine counter-ions (chlorpromazine hydrochloride, thioridazine hydrochloride and trifluoperazine dihydrochloride, respectively) through the formation of three different complexes (1–3) was investigated. We explored whether the selected counter-ions and complexes might affect redox homeostasis and genome integrity of normal human blood cells, and induce an inhibition of Na+/K+-ATPase and AChE at pharmacologically relevant doses. Our results have shown that counter-ions and complexes did not affect the activity of Na+/K+-ATPase, while AChE activity was inhibited in a dose-dependent manner. All investigated compounds disturbed the viability and redox homeostasis of lymphocytes. Complexes 1 and 2 displayed potent cytotoxic and prooxidant action while complex 3 behaved as a weaker genotoxic inducer. Still, the tested complexes appeared to be less genotoxic and more cytostatic than the corresponding counter-ions. The effects of selected complexes were also tested in PC12 and U2OS cancer cells with special attention being given to the ability of phenothiazines to affect dopamine D2 receptors. Using the confocal laser scanning microscopy, we observed that all the complexes reduced cell viability. Although all investigated complexes have been bound to the dopamine receptor D2-eGFP, only complex 3 reduced its surface density and increased its lateral mobility in investigated cell lines. Albeit the role of alternative targets for complex 3 cannot be ruled out, its effects should be further examined as potential treatment strategy against cancer cells that overexpress D2.
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents
VL  - 23
IS  - 5
SP  - 689
EP  - 704
DO  - 10.1007/s00775-018-1560-x
ER  - 
@article{
author = "Leskovac, Andreja and Petrović, Sandra and Lazarević-Pašti, Tamara and Krstić, Milena P. and Vasić, Vesna M.",
year = "2018",
abstract = "In recent years, the search for effective anticancer compounds based on transition metal complexes has been the focus of medical investigations. The synergy between the ruthenium(II) and N-alkylphenothiazine counter-ions (chlorpromazine hydrochloride, thioridazine hydrochloride and trifluoperazine dihydrochloride, respectively) through the formation of three different complexes (1–3) was investigated. We explored whether the selected counter-ions and complexes might affect redox homeostasis and genome integrity of normal human blood cells, and induce an inhibition of Na+/K+-ATPase and AChE at pharmacologically relevant doses. Our results have shown that counter-ions and complexes did not affect the activity of Na+/K+-ATPase, while AChE activity was inhibited in a dose-dependent manner. All investigated compounds disturbed the viability and redox homeostasis of lymphocytes. Complexes 1 and 2 displayed potent cytotoxic and prooxidant action while complex 3 behaved as a weaker genotoxic inducer. Still, the tested complexes appeared to be less genotoxic and more cytostatic than the corresponding counter-ions. The effects of selected complexes were also tested in PC12 and U2OS cancer cells with special attention being given to the ability of phenothiazines to affect dopamine D2 receptors. Using the confocal laser scanning microscopy, we observed that all the complexes reduced cell viability. Although all investigated complexes have been bound to the dopamine receptor D2-eGFP, only complex 3 reduced its surface density and increased its lateral mobility in investigated cell lines. Albeit the role of alternative targets for complex 3 cannot be ruled out, its effects should be further examined as potential treatment strategy against cancer cells that overexpress D2.",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents",
volume = "23",
number = "5",
pages = "689-704",
doi = "10.1007/s00775-018-1560-x"
}
Leskovac, A., Petrović, S., Lazarević-Pašti, T., Krstić, M. P.,& Vasić, V. M.. (2018). Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents. in JBIC Journal of Biological Inorganic Chemistry, 23(5), 689-704.
https://doi.org/10.1007/s00775-018-1560-x
Leskovac A, Petrović S, Lazarević-Pašti T, Krstić MP, Vasić VM. Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents. in JBIC Journal of Biological Inorganic Chemistry. 2018;23(5):689-704.
doi:10.1007/s00775-018-1560-x .
Leskovac, Andreja, Petrović, Sandra, Lazarević-Pašti, Tamara, Krstić, Milena P., Vasić, Vesna M., "Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents" in JBIC Journal of Biological Inorganic Chemistry, 23, no. 5 (2018):689-704,
https://doi.org/10.1007/s00775-018-1560-x . .
5
3
2

Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation

Bogdanović, Una; Dimitrijević, Suzana I.; Škapin, Srečo Davor; Popović, Maja; Rakočević, Zlatko Lj.; Leskovac, Andreja; Petrović, Sandra; Stoiljković, Milovan; Vodnik, Vesna

(2018)

TY  - JOUR
AU  - Bogdanović, Una
AU  - Dimitrijević, Suzana I.
AU  - Škapin, Srečo Davor
AU  - Popović, Maja
AU  - Rakočević, Zlatko Lj.
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Stoiljković, Milovan
AU  - Vodnik, Vesna
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0928493117326449
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7799
AB  - Copper nanoparticles (Cu NPs) have proven to own excellent antimicrobial efficacy, but the problems of easy oxidation and aggregation limit their practical application. Here, nanocomposite based on polyaniline (PANI) and Cu NPs solved this problem and brought additional physicochemical properties that are markedly advantageous for antimicrobial applications. Current work exploits this potential, to examine its time- and concentration-dependent antimicrobial activity, employing E. coli, S. aureus, and C. albicans as a model microbial species. Regarding the presence of polaronic charge carriers in the fibrous polyaniline network, effects of Cu NPs’ size and their partially oxidized surfaces (the data were confirmed by HRTEM, FESEM, XRD, Raman and XPS analysis), as well as rapid copper ions release, Cu-PANI nanocomposite showed efficient bactericidal and fungicidal activities at the concentrations ≤1 ppm, within the incubation time of 2 h. Beside the quantitative analysis, the high levels of cellular disruption for all tested microbes were evidenced by atomic force microscopy. Moreover, the minimum inhibitory and bactericidal concentrations of the Cu-PANI nanocomposite were lower than those reported for other nanocomposites. Using such low concentrations is recognized as a good way to avoid its toxicity toward the environment. For this purpose, Cu-PANI nanocomposite is tested for its genotoxicity and influence on the oxidative status of the human cells in vitro.
T2  - Materials Science and Engineering: C
T1  - Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation
VL  - 93
SP  - 49
EP  - 60
DO  - 10.1016/j.msec.2018.07.067
ER  - 
@article{
author = "Bogdanović, Una and Dimitrijević, Suzana I. and Škapin, Srečo Davor and Popović, Maja and Rakočević, Zlatko Lj. and Leskovac, Andreja and Petrović, Sandra and Stoiljković, Milovan and Vodnik, Vesna",
year = "2018",
abstract = "Copper nanoparticles (Cu NPs) have proven to own excellent antimicrobial efficacy, but the problems of easy oxidation and aggregation limit their practical application. Here, nanocomposite based on polyaniline (PANI) and Cu NPs solved this problem and brought additional physicochemical properties that are markedly advantageous for antimicrobial applications. Current work exploits this potential, to examine its time- and concentration-dependent antimicrobial activity, employing E. coli, S. aureus, and C. albicans as a model microbial species. Regarding the presence of polaronic charge carriers in the fibrous polyaniline network, effects of Cu NPs’ size and their partially oxidized surfaces (the data were confirmed by HRTEM, FESEM, XRD, Raman and XPS analysis), as well as rapid copper ions release, Cu-PANI nanocomposite showed efficient bactericidal and fungicidal activities at the concentrations ≤1 ppm, within the incubation time of 2 h. Beside the quantitative analysis, the high levels of cellular disruption for all tested microbes were evidenced by atomic force microscopy. Moreover, the minimum inhibitory and bactericidal concentrations of the Cu-PANI nanocomposite were lower than those reported for other nanocomposites. Using such low concentrations is recognized as a good way to avoid its toxicity toward the environment. For this purpose, Cu-PANI nanocomposite is tested for its genotoxicity and influence on the oxidative status of the human cells in vitro.",
journal = "Materials Science and Engineering: C",
title = "Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation",
volume = "93",
pages = "49-60",
doi = "10.1016/j.msec.2018.07.067"
}
Bogdanović, U., Dimitrijević, S. I., Škapin, S. D., Popović, M., Rakočević, Z. Lj., Leskovac, A., Petrović, S., Stoiljković, M.,& Vodnik, V.. (2018). Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation. in Materials Science and Engineering: C, 93, 49-60.
https://doi.org/10.1016/j.msec.2018.07.067
Bogdanović U, Dimitrijević SI, Škapin SD, Popović M, Rakočević ZL, Leskovac A, Petrović S, Stoiljković M, Vodnik V. Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation. in Materials Science and Engineering: C. 2018;93:49-60.
doi:10.1016/j.msec.2018.07.067 .
Bogdanović, Una, Dimitrijević, Suzana I., Škapin, Srečo Davor, Popović, Maja, Rakočević, Zlatko Lj., Leskovac, Andreja, Petrović, Sandra, Stoiljković, Milovan, Vodnik, Vesna, "Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation" in Materials Science and Engineering: C, 93 (2018):49-60,
https://doi.org/10.1016/j.msec.2018.07.067 . .
17
14
14

The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach

Bondžić, Aleksandra M.; Čolović, Mirjana B.; Janjić, Goran V.; Zarić, Božidarka; Petrović, Sandra; Krstić, Danijela Z.; Marzo, Tiziano; Messori, Luigi; Vasić, Vesna M.

(2017)

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Čolović, Mirjana B.
AU  - Janjić, Goran V.
AU  - Zarić, Božidarka
AU  - Petrović, Sandra
AU  - Krstić, Danijela Z.
AU  - Marzo, Tiziano
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1648
AB  - The in vitro effects of oxo-bridged binuclear gold(III) complexes, i.e., [(bipy2Me)(2)Au-2(mu-O)(2)][PF6](2) (Auoxo6), Au-2[(bipydmb-H)(2)(mu-O)][PF6] (Au(2)bipyC) and [Au-2(phen(2Me))(2)(mu-O)(2)](PF6)(2) (Au(2)phen) on Na/K-ATPase, purified from the porcine cerebral cortex, were investigated. All three studied gold complexes inhibited the enzyme activity in a concentration-dependent manner achieving IC50 values in the low micromolar range. Kinetic analysis suggested an uncompetitive mode of inhibition for Auoxo6 and Au(2)bipyC, and a mixed type one for Au(2)phen. Docking studies indicated that the inhibitory actions of all tested complexes are related to E2-P enzyme conformation binding to ion channel and intracellular part between N and P sub-domain. In addition, Au(2)phen was able to inhibit the enzyme by interacting with its extracellular part as well. Toxic effects of the gold(III) complexes were evaluated in vitro by following lactate dehydrogenase activity in rat brain synaptosomes and incidence of micronuclei and cytokinesis-block proliferation index in cultivated human lymphocytes. All investigated complexes turned out to induce cytogenetic damage consisting of a significant decrease in cell proliferation and an increase in micronuclei in a dose-dependent manner. On the other hand, lactate dehydrogenase activity, an indicator of membrane integrity/viability, was not affected by Auoxo6 and Au(2)bipyC, while Au(2)phen slightly modified its activity.
T2  - Journal of Biological Inorganic Chemistry
T1  - The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach
VL  - 22
IS  - 6
SP  - 819
EP  - 832
DO  - 10.1007/s00775-017-1460-5
ER  - 
@article{
author = "Bondžić, Aleksandra M. and Čolović, Mirjana B. and Janjić, Goran V. and Zarić, Božidarka and Petrović, Sandra and Krstić, Danijela Z. and Marzo, Tiziano and Messori, Luigi and Vasić, Vesna M.",
year = "2017",
abstract = "The in vitro effects of oxo-bridged binuclear gold(III) complexes, i.e., [(bipy2Me)(2)Au-2(mu-O)(2)][PF6](2) (Auoxo6), Au-2[(bipydmb-H)(2)(mu-O)][PF6] (Au(2)bipyC) and [Au-2(phen(2Me))(2)(mu-O)(2)](PF6)(2) (Au(2)phen) on Na/K-ATPase, purified from the porcine cerebral cortex, were investigated. All three studied gold complexes inhibited the enzyme activity in a concentration-dependent manner achieving IC50 values in the low micromolar range. Kinetic analysis suggested an uncompetitive mode of inhibition for Auoxo6 and Au(2)bipyC, and a mixed type one for Au(2)phen. Docking studies indicated that the inhibitory actions of all tested complexes are related to E2-P enzyme conformation binding to ion channel and intracellular part between N and P sub-domain. In addition, Au(2)phen was able to inhibit the enzyme by interacting with its extracellular part as well. Toxic effects of the gold(III) complexes were evaluated in vitro by following lactate dehydrogenase activity in rat brain synaptosomes and incidence of micronuclei and cytokinesis-block proliferation index in cultivated human lymphocytes. All investigated complexes turned out to induce cytogenetic damage consisting of a significant decrease in cell proliferation and an increase in micronuclei in a dose-dependent manner. On the other hand, lactate dehydrogenase activity, an indicator of membrane integrity/viability, was not affected by Auoxo6 and Au(2)bipyC, while Au(2)phen slightly modified its activity.",
journal = "Journal of Biological Inorganic Chemistry",
title = "The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach",
volume = "22",
number = "6",
pages = "819-832",
doi = "10.1007/s00775-017-1460-5"
}
Bondžić, A. M., Čolović, M. B., Janjić, G. V., Zarić, B., Petrović, S., Krstić, D. Z., Marzo, T., Messori, L.,& Vasić, V. M.. (2017). The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach. in Journal of Biological Inorganic Chemistry, 22(6), 819-832.
https://doi.org/10.1007/s00775-017-1460-5
Bondžić AM, Čolović MB, Janjić GV, Zarić B, Petrović S, Krstić DZ, Marzo T, Messori L, Vasić VM. The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach. in Journal of Biological Inorganic Chemistry. 2017;22(6):819-832.
doi:10.1007/s00775-017-1460-5 .
Bondžić, Aleksandra M., Čolović, Mirjana B., Janjić, Goran V., Zarić, Božidarka, Petrović, Sandra, Krstić, Danijela Z., Marzo, Tiziano, Messori, Luigi, Vasić, Vesna M., "The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach" in Journal of Biological Inorganic Chemistry, 22, no. 6 (2017):819-832,
https://doi.org/10.1007/s00775-017-1460-5 . .
5
4
4

Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro

Lazovic, Sasa; Leskovac, Andreja; Petrović, Sandra; Senerović, Lidija; Krivokapić, Nevena; Mitrović, Tatjana; Božović, Nikola; Vasić, Vesna M.; Nikodinović-Runić, Jasmina

(2017)

TY  - JOUR
AU  - Lazovic, Sasa
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Senerović, Lidija
AU  - Krivokapić, Nevena
AU  - Mitrović, Tatjana
AU  - Božović, Nikola
AU  - Vasić, Vesna M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1370
AB  - It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((52)-4-methoxy-5-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5H-2,2-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.
T2  - Experimental and Toxicologic Pathology
T1  - Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro
VL  - 69
IS  - 1
SP  - 55
EP  - 62
DO  - 10.1016/j.etp.2016.11.003
ER  - 
@article{
author = "Lazovic, Sasa and Leskovac, Andreja and Petrović, Sandra and Senerović, Lidija and Krivokapić, Nevena and Mitrović, Tatjana and Božović, Nikola and Vasić, Vesna M. and Nikodinović-Runić, Jasmina",
year = "2017",
abstract = "It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((52)-4-methoxy-5-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5H-2,2-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.",
journal = "Experimental and Toxicologic Pathology",
title = "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro",
volume = "69",
number = "1",
pages = "55-62",
doi = "10.1016/j.etp.2016.11.003"
}
Lazovic, S., Leskovac, A., Petrović, S., Senerović, L., Krivokapić, N., Mitrović, T., Božović, N., Vasić, V. M.,& Nikodinović-Runić, J.. (2017). Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology, 69(1), 55-62.
https://doi.org/10.1016/j.etp.2016.11.003
Lazovic S, Leskovac A, Petrović S, Senerović L, Krivokapić N, Mitrović T, Božović N, Vasić VM, Nikodinović-Runić J. Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology. 2017;69(1):55-62.
doi:10.1016/j.etp.2016.11.003 .
Lazovic, Sasa, Leskovac, Andreja, Petrović, Sandra, Senerović, Lidija, Krivokapić, Nevena, Mitrović, Tatjana, Božović, Nikola, Vasić, Vesna M., Nikodinović-Runić, Jasmina, "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro" in Experimental and Toxicologic Pathology, 69, no. 1 (2017):55-62,
https://doi.org/10.1016/j.etp.2016.11.003 . .
3
3
3

The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro

Petrović, Sandra; Vasić, Vesna M.; Mitrović, Tatjana; Lazovic, Sasa; Leskovac, Andreja

(2017)

TY  - JOUR
AU  - Petrović, Sandra
AU  - Vasić, Vesna M.
AU  - Mitrović, Tatjana
AU  - Lazovic, Sasa
AU  - Leskovac, Andreja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1506
AB  - Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.
T2  - Arhiv za higijenu rada i toksikologiju
T1  - The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro
VL  - 68
IS  - 1
SP  - 1
EP  - 8
DO  - 10.1515/aiht-2017-68-2897
ER  - 
@article{
author = "Petrović, Sandra and Vasić, Vesna M. and Mitrović, Tatjana and Lazovic, Sasa and Leskovac, Andreja",
year = "2017",
abstract = "Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro",
volume = "68",
number = "1",
pages = "1-8",
doi = "10.1515/aiht-2017-68-2897"
}
Petrović, S., Vasić, V. M., Mitrović, T., Lazovic, S.,& Leskovac, A.. (2017). The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro. in Arhiv za higijenu rada i toksikologiju, 68(1), 1-8.
https://doi.org/10.1515/aiht-2017-68-2897
Petrović S, Vasić VM, Mitrović T, Lazovic S, Leskovac A. The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro. in Arhiv za higijenu rada i toksikologiju. 2017;68(1):1-8.
doi:10.1515/aiht-2017-68-2897 .
Petrović, Sandra, Vasić, Vesna M., Mitrović, Tatjana, Lazovic, Sasa, Leskovac, Andreja, "The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro" in Arhiv za higijenu rada i toksikologiju, 68, no. 1 (2017):1-8,
https://doi.org/10.1515/aiht-2017-68-2897 . .
7
5
5

Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs

Lazarević-Pašti, Tamara; Leskovac, Andreja; Momić, Tatjana; Petrović, Sandra; Vasić, Vesna M.

(2017)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Leskovac, Andreja
AU  - Momić, Tatjana
AU  - Petrović, Sandra
AU  - Vasić, Vesna M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1796
AB  - Background: Acetylcholinesterase (AChE) is involved in the termination of impulse transmission by rapid hydrolysis of the neurotransmitter acetylcholine in numerous cholinergic pathways in the central and peripheral nervous systems. The enzyme inactivation leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. Hence, acetylcholinesterase inhibitors, interacting with the enzyme as their primary target, are applied as relevant drugs for different neurodegenerative diseases (such as Alzheimers and Parkinsons) as well as toxins. At the same time, there are increasing evidence that in non-neuronal context, AChE is involved in the regulation of cell proliferation, differentiation, apoptosis and cell-cell interaction. An irregular expression of AChE has been found in different types of tumors, suggesting the involvement of AChE in the regulation of tumor development. Having all this in mind, there is a possibility that some AChE inhibitors could be used as anti-cancer agents. Objective: This contribution will discuss a broad range of possible application of different AChE inhibitors as drugs, from well-known anti-Alzheimers disease drugs to their use in cancer treatment in future. Emphasis will be put on various known AChE inhibitors classes, whose application as drugs could be controversy, as well as on newly investigated natural products, which can also modulate AChE activity. Conclusion: It is not clear a patient treated for neurodegenerative condition prone to increased risk for some types of cancer and vice versa. This is necessary to keep in mind during rational drug design process for all therapies, which are based on AChE as a target molecule.
T2  - Current Medicinal Chemistry
T1  - Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs
VL  - 24
IS  - 30
SP  - 3283
EP  - 3309
DO  - 10.2174/0929867324666170705123509
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Leskovac, Andreja and Momić, Tatjana and Petrović, Sandra and Vasić, Vesna M.",
year = "2017",
abstract = "Background: Acetylcholinesterase (AChE) is involved in the termination of impulse transmission by rapid hydrolysis of the neurotransmitter acetylcholine in numerous cholinergic pathways in the central and peripheral nervous systems. The enzyme inactivation leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. Hence, acetylcholinesterase inhibitors, interacting with the enzyme as their primary target, are applied as relevant drugs for different neurodegenerative diseases (such as Alzheimers and Parkinsons) as well as toxins. At the same time, there are increasing evidence that in non-neuronal context, AChE is involved in the regulation of cell proliferation, differentiation, apoptosis and cell-cell interaction. An irregular expression of AChE has been found in different types of tumors, suggesting the involvement of AChE in the regulation of tumor development. Having all this in mind, there is a possibility that some AChE inhibitors could be used as anti-cancer agents. Objective: This contribution will discuss a broad range of possible application of different AChE inhibitors as drugs, from well-known anti-Alzheimers disease drugs to their use in cancer treatment in future. Emphasis will be put on various known AChE inhibitors classes, whose application as drugs could be controversy, as well as on newly investigated natural products, which can also modulate AChE activity. Conclusion: It is not clear a patient treated for neurodegenerative condition prone to increased risk for some types of cancer and vice versa. This is necessary to keep in mind during rational drug design process for all therapies, which are based on AChE as a target molecule.",
journal = "Current Medicinal Chemistry",
title = "Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs",
volume = "24",
number = "30",
pages = "3283-3309",
doi = "10.2174/0929867324666170705123509"
}
Lazarević-Pašti, T., Leskovac, A., Momić, T., Petrović, S.,& Vasić, V. M.. (2017). Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs. in Current Medicinal Chemistry, 24(30), 3283-3309.
https://doi.org/10.2174/0929867324666170705123509
Lazarević-Pašti T, Leskovac A, Momić T, Petrović S, Vasić VM. Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs. in Current Medicinal Chemistry. 2017;24(30):3283-3309.
doi:10.2174/0929867324666170705123509 .
Lazarević-Pašti, Tamara, Leskovac, Andreja, Momić, Tatjana, Petrović, Sandra, Vasić, Vesna M., "Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs" in Current Medicinal Chemistry, 24, no. 30 (2017):3283-3309,
https://doi.org/10.2174/0929867324666170705123509 . .
7
53
46
45

Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients

Petrović, Sandra; Leskovac, Andreja; Joksić, Ivana; Vujic, Dragana; Valenta-Šobot, Ana; Filipović, Jelena G.; Joksić, Gordana

(2015)

TY  - JOUR
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Vujic, Dragana
AU  - Valenta-Šobot, Ana
AU  - Filipović, Jelena G.
AU  - Joksić, Gordana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/777
AB  - Fanconi anemia (FA) is a rare genetically heterogeneous disorder associated with bone marrow failure, birth defects and cancer susceptibility. Apart from the disease-causing mutations in FANC genes, the identification of specific DNA variations, such as single nucleotide polymorphisms (SNPs), in other candidate genes may lead to a better clinical description of this condition enabling individualized treatment with improvement of the prognosis. In this study, we have assessed 95 SNPs located in 52 key genes involved in base excision repair (BER), nucleotide excision repair (NER), mismatch repair (MMR), double strand break (DSB) repair and cell cycle control using a DNA repair chip (Asper Biotech, Estonia) which includes most of the common variants for the candidate genes. The SNP genotyping was performed in five FA-D2 patients and in one FA-A patient. The polymorphisms studied were synonymous (n=10), nonsynonymous (missense) (n=52) and in non-coding regions of the genome (introns and 5 and 3 untranslated regions (UTR)) (n=33). Polymorphisms found at the homozygous state are selected for further analysis. Our results have shown a significant inter-individual variability among patients in the type and the frequency of SNPs and also elucidate the need for further studies of polymorphisms located in ATM, APEX APE 1, XRCC1, ERCC2, MSH3, PARP4, NBS1, BARD1, CDKN1B, TP53 and TP53BP1 which may be of great importance for better clinical description of FA. In addition, the present report recommends the use of SNPs as predictive and prognostic genetic markers to individualize therapy of FA patients.
T2  - Genetika (Beograd)
T1  - Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients
VL  - 47
IS  - 2
SP  - 695
EP  - 710
DO  - 10.2298/GENSR1502695P
ER  - 
@article{
author = "Petrović, Sandra and Leskovac, Andreja and Joksić, Ivana and Vujic, Dragana and Valenta-Šobot, Ana and Filipović, Jelena G. and Joksić, Gordana",
year = "2015",
abstract = "Fanconi anemia (FA) is a rare genetically heterogeneous disorder associated with bone marrow failure, birth defects and cancer susceptibility. Apart from the disease-causing mutations in FANC genes, the identification of specific DNA variations, such as single nucleotide polymorphisms (SNPs), in other candidate genes may lead to a better clinical description of this condition enabling individualized treatment with improvement of the prognosis. In this study, we have assessed 95 SNPs located in 52 key genes involved in base excision repair (BER), nucleotide excision repair (NER), mismatch repair (MMR), double strand break (DSB) repair and cell cycle control using a DNA repair chip (Asper Biotech, Estonia) which includes most of the common variants for the candidate genes. The SNP genotyping was performed in five FA-D2 patients and in one FA-A patient. The polymorphisms studied were synonymous (n=10), nonsynonymous (missense) (n=52) and in non-coding regions of the genome (introns and 5 and 3 untranslated regions (UTR)) (n=33). Polymorphisms found at the homozygous state are selected for further analysis. Our results have shown a significant inter-individual variability among patients in the type and the frequency of SNPs and also elucidate the need for further studies of polymorphisms located in ATM, APEX APE 1, XRCC1, ERCC2, MSH3, PARP4, NBS1, BARD1, CDKN1B, TP53 and TP53BP1 which may be of great importance for better clinical description of FA. In addition, the present report recommends the use of SNPs as predictive and prognostic genetic markers to individualize therapy of FA patients.",
journal = "Genetika (Beograd)",
title = "Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients",
volume = "47",
number = "2",
pages = "695-710",
doi = "10.2298/GENSR1502695P"
}
Petrović, S., Leskovac, A., Joksić, I., Vujic, D., Valenta-Šobot, A., Filipović, J. G.,& Joksić, G.. (2015). Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients. in Genetika (Beograd), 47(2), 695-710.
https://doi.org/10.2298/GENSR1502695P
Petrović S, Leskovac A, Joksić I, Vujic D, Valenta-Šobot A, Filipović JG, Joksić G. Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients. in Genetika (Beograd). 2015;47(2):695-710.
doi:10.2298/GENSR1502695P .
Petrović, Sandra, Leskovac, Andreja, Joksić, Ivana, Vujic, Dragana, Valenta-Šobot, Ana, Filipović, Jelena G., Joksić, Gordana, "Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients" in Genetika (Beograd), 47, no. 2 (2015):695-710,
https://doi.org/10.2298/GENSR1502695P . .

Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes

Petrović, Voin; Petrović, Sandra; Joksić, Gordana; Savić, Jasmina; Čolović, Mirjana B.; Cinellu, Maria Agostina; Massai, Lara; Messori, Luigi; Vasić, Vesna M.

(2014)

TY  - JOUR
AU  - Petrović, Voin
AU  - Petrović, Sandra
AU  - Joksić, Gordana
AU  - Savić, Jasmina
AU  - Čolović, Mirjana B.
AU  - Cinellu, Maria Agostina
AU  - Massai, Lara
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/140
AB  - Na+/K+-ATPase is in charge of maintaining the ionic and osmotic intracellular balance by using ATP as an energy source to drive excess Na+ ions out of the cell in exchange for K+ ions. We explored whether three representative cytotoxic gold(III) compounds might interfere with Na+/K+-ATPase and cause its inhibition at pharmacologically relevant concentrations. The tested complexes were [Au(bipy)(OH)(2)][PF6] (bipy = 2,2-bipyridine), [Au (py(dmb)-H)(CH3COO)(2)] (py(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-pyridine), and [Au(bipy(dmb)-H)(OH)][PF6] (bipy(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-2,2-bipyridine). We found that all of them caused a pronounced and similar inhibition of Na+/K+-ATPase activity. Inhibition was found to be non-competitive and reversible. Remarkably, treatment with cysteine resulted in reversal or prevention of Na+/K+-ATPase inhibition. It is very likely that the described effects may contribute to the overall cytotoxic profile of these gold complexes. (C) 2014 Elsevier Inc. All rights reserved.
T2  - Journal of Inorganic Biochemistry
T1  - Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes
VL  - 140
SP  - 228
EP  - 235
DO  - 10.1016/j.jinorgbio.2014.07.015
ER  - 
@article{
author = "Petrović, Voin and Petrović, Sandra and Joksić, Gordana and Savić, Jasmina and Čolović, Mirjana B. and Cinellu, Maria Agostina and Massai, Lara and Messori, Luigi and Vasić, Vesna M.",
year = "2014",
abstract = "Na+/K+-ATPase is in charge of maintaining the ionic and osmotic intracellular balance by using ATP as an energy source to drive excess Na+ ions out of the cell in exchange for K+ ions. We explored whether three representative cytotoxic gold(III) compounds might interfere with Na+/K+-ATPase and cause its inhibition at pharmacologically relevant concentrations. The tested complexes were [Au(bipy)(OH)(2)][PF6] (bipy = 2,2-bipyridine), [Au (py(dmb)-H)(CH3COO)(2)] (py(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-pyridine), and [Au(bipy(dmb)-H)(OH)][PF6] (bipy(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-2,2-bipyridine). We found that all of them caused a pronounced and similar inhibition of Na+/K+-ATPase activity. Inhibition was found to be non-competitive and reversible. Remarkably, treatment with cysteine resulted in reversal or prevention of Na+/K+-ATPase inhibition. It is very likely that the described effects may contribute to the overall cytotoxic profile of these gold complexes. (C) 2014 Elsevier Inc. All rights reserved.",
journal = "Journal of Inorganic Biochemistry",
title = "Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes",
volume = "140",
pages = "228-235",
doi = "10.1016/j.jinorgbio.2014.07.015"
}
Petrović, V., Petrović, S., Joksić, G., Savić, J., Čolović, M. B., Cinellu, M. A., Massai, L., Messori, L.,& Vasić, V. M.. (2014). Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes. in Journal of Inorganic Biochemistry, 140, 228-235.
https://doi.org/10.1016/j.jinorgbio.2014.07.015
Petrović V, Petrović S, Joksić G, Savić J, Čolović MB, Cinellu MA, Massai L, Messori L, Vasić VM. Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes. in Journal of Inorganic Biochemistry. 2014;140:228-235.
doi:10.1016/j.jinorgbio.2014.07.015 .
Petrović, Voin, Petrović, Sandra, Joksić, Gordana, Savić, Jasmina, Čolović, Mirjana B., Cinellu, Maria Agostina, Massai, Lara, Messori, Luigi, Vasić, Vesna M., "Inhibition of Na+/K+-ATPase and cytotoxicity of a few selected gold(III) complexes" in Journal of Inorganic Biochemistry, 140 (2014):228-235,
https://doi.org/10.1016/j.jinorgbio.2014.07.015 . .
9
8
9

Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia

Vujić, Dragana; Petrović, Sandra; Lazić, Emilija; Kuzmanović, Miloš; Leskovac, Andreja; Joksić, Ivana; Mićić, Dragan; Jovanović, Ankica; Zečević, Željko; Guć-Šćekić, Marija; Ćirković, Sanja; Joksić, Gordana

(2014)

TY  - JOUR
AU  - Vujić, Dragana
AU  - Petrović, Sandra
AU  - Lazić, Emilija
AU  - Kuzmanović, Miloš
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Mićić, Dragan
AU  - Jovanović, Ankica
AU  - Zečević, Željko
AU  - Guć-Šćekić, Marija
AU  - Ćirković, Sanja
AU  - Joksić, Gordana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5928
AB  - To investigate genetic subtypes of inherited bone marrow failure syndrome Fanconi anemia (FA) in Sebia. FA-D2 subtype was found to be the most frequent genetic subtype among investigated FA patients; specific observations of FA-D2 phenotype are pointed out. Several biological endpoints of FA cells in vitro such as radiation-induced level of lymphocyte micronuclei (radiosensitivity), base line and radiation induced level of the DNA double strand breaks (DSBs), leukocyte apoptosis, and telomere capping function were assessed. The results indicate that all FA-D2 patients display radioresistant in vitro response, which is seen as significantly reduced yield of radiation-induced micronuclei. On the contrary, FA-A patients display radiosensitive in vitro response seen as increased number of radiation-induced micronuclei (MN). A massive elimination of irradiated cells via apoptosis is found in both FA-A and FA-D2 subtypes. In FA-A subtype apoptosis positively relates with the yield of radiation-induced MN, whereas in FA-D2 subtype apoptosis relates with a high percentage of cells carrying dysfunctional telomeres. The present results unequivocally demonstrate that cytokinesis-block micronucleus (CBMN) assay and analyses of telomere capping function can be used to distinguish FA-D2 and FA-A complementation groups. Considering all biological endpoints were analyzed, it can be concluded that all FA patients are radiosensitive, regardless of their complementation group. Thus, using CBMN test and telomere capping function analysis can discriminate FA-A from FA-D2 complementation groups, which could be important for assessment the conditioning regimens prior to bone marrow transplantation.
T2  - Indian Journal of Pediatrics
T1  - Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia
VL  - 81
IS  - 3
SP  - 260
EP  - 265
DO  - 10.1007/s12098-013-1284-4
ER  - 
@article{
author = "Vujić, Dragana and Petrović, Sandra and Lazić, Emilija and Kuzmanović, Miloš and Leskovac, Andreja and Joksić, Ivana and Mićić, Dragan and Jovanović, Ankica and Zečević, Željko and Guć-Šćekić, Marija and Ćirković, Sanja and Joksić, Gordana",
year = "2014",
abstract = "To investigate genetic subtypes of inherited bone marrow failure syndrome Fanconi anemia (FA) in Sebia. FA-D2 subtype was found to be the most frequent genetic subtype among investigated FA patients; specific observations of FA-D2 phenotype are pointed out. Several biological endpoints of FA cells in vitro such as radiation-induced level of lymphocyte micronuclei (radiosensitivity), base line and radiation induced level of the DNA double strand breaks (DSBs), leukocyte apoptosis, and telomere capping function were assessed. The results indicate that all FA-D2 patients display radioresistant in vitro response, which is seen as significantly reduced yield of radiation-induced micronuclei. On the contrary, FA-A patients display radiosensitive in vitro response seen as increased number of radiation-induced micronuclei (MN). A massive elimination of irradiated cells via apoptosis is found in both FA-A and FA-D2 subtypes. In FA-A subtype apoptosis positively relates with the yield of radiation-induced MN, whereas in FA-D2 subtype apoptosis relates with a high percentage of cells carrying dysfunctional telomeres. The present results unequivocally demonstrate that cytokinesis-block micronucleus (CBMN) assay and analyses of telomere capping function can be used to distinguish FA-D2 and FA-A complementation groups. Considering all biological endpoints were analyzed, it can be concluded that all FA patients are radiosensitive, regardless of their complementation group. Thus, using CBMN test and telomere capping function analysis can discriminate FA-A from FA-D2 complementation groups, which could be important for assessment the conditioning regimens prior to bone marrow transplantation.",
journal = "Indian Journal of Pediatrics",
title = "Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia",
volume = "81",
number = "3",
pages = "260-265",
doi = "10.1007/s12098-013-1284-4"
}
Vujić, D., Petrović, S., Lazić, E., Kuzmanović, M., Leskovac, A., Joksić, I., Mićić, D., Jovanović, A., Zečević, Ž., Guć-Šćekić, M., Ćirković, S.,& Joksić, G.. (2014). Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia. in Indian Journal of Pediatrics, 81(3), 260-265.
https://doi.org/10.1007/s12098-013-1284-4
Vujić D, Petrović S, Lazić E, Kuzmanović M, Leskovac A, Joksić I, Mićić D, Jovanović A, Zečević Ž, Guć-Šćekić M, Ćirković S, Joksić G. Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia. in Indian Journal of Pediatrics. 2014;81(3):260-265.
doi:10.1007/s12098-013-1284-4 .
Vujić, Dragana, Petrović, Sandra, Lazić, Emilija, Kuzmanović, Miloš, Leskovac, Andreja, Joksić, Ivana, Mićić, Dragan, Jovanović, Ankica, Zečević, Željko, Guć-Šćekić, Marija, Ćirković, Sanja, Joksić, Gordana, "Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia" in Indian Journal of Pediatrics, 81, no. 3 (2014):260-265,
https://doi.org/10.1007/s12098-013-1284-4 . .
2
3
3

Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts

Leskovac, Andreja; Petrović, Sandra; Guć-Šćekić, Marija; Vujic, Dragana; Joksić, Gordana

(2014)

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Guć-Šćekić, Marija
AU  - Vujic, Dragana
AU  - Joksić, Gordana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5986
AB  - Purpose: As the Fanconi anemia (FA) pathway is required for appropriate cell cycle progression through mitosis and the completion of cell division, the aim of the present study was to determine the destiny of FA cells after irradiation in vitro and to elucidate any difference in radiosensitivity between FA and control cells. Materials and methods: Analyses of phosphorylated histone H2AX (gamma-H2AX) foci, micronuclei formation and cell cycle analysis were performed in unirradiated (0 min) and irradiated primary FA fibroblasts and in a control group at different post-irradiation times (30 min, 2 h, 5 h and 24 h). Results: The accumulation of gamma-H2AX foci in irradiated FA fibroblasts was observed. At 24 h post-irradiation, 57% of FA cells were gamma-H2AX foci-positive, significantly higher than in the control (p LT 0.01). The cell cycle analysis has shown the transient G2/M arrest in irradiated FA fibroblasts. The portion of cells in the G2/M phase showed initial increase at 30 min post-irradiation and afterwards decreased over time reaching the pretreatment level 24 h after irradiation. Irradiated FA fibroblasts progressed to abnormal mitosis, as is shown by the production of cells with different nuclear morphologies from binucleated to multinucleated surrounded with micronuclei, and also by a high percentage of foci-positive micronuclei. The majority of radiation-induced micronuclei were gamma-H2AX foci-positive, indicating that radiation-induced micronuclei contain fragments of damaged chromosomes. In contrast, in the control group, most of the micronuclei were classified as gamma-H2AX foci-negative, which indicates that cells with unrepaired damage were blocked before entering mitosis. Conclusion: The results clearly indicate that mitotic catastrophe might be an important cell-death mechanism involved in the response of FA fibroblasts to ionizing radiation.
T2  - International Journal of Radiation Biology
T1  - Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts
VL  - 90
IS  - 5
SP  - 373
EP  - 381
DO  - 10.3109/09553002.2014.892224
ER  - 
@article{
author = "Leskovac, Andreja and Petrović, Sandra and Guć-Šćekić, Marija and Vujic, Dragana and Joksić, Gordana",
year = "2014",
abstract = "Purpose: As the Fanconi anemia (FA) pathway is required for appropriate cell cycle progression through mitosis and the completion of cell division, the aim of the present study was to determine the destiny of FA cells after irradiation in vitro and to elucidate any difference in radiosensitivity between FA and control cells. Materials and methods: Analyses of phosphorylated histone H2AX (gamma-H2AX) foci, micronuclei formation and cell cycle analysis were performed in unirradiated (0 min) and irradiated primary FA fibroblasts and in a control group at different post-irradiation times (30 min, 2 h, 5 h and 24 h). Results: The accumulation of gamma-H2AX foci in irradiated FA fibroblasts was observed. At 24 h post-irradiation, 57% of FA cells were gamma-H2AX foci-positive, significantly higher than in the control (p LT 0.01). The cell cycle analysis has shown the transient G2/M arrest in irradiated FA fibroblasts. The portion of cells in the G2/M phase showed initial increase at 30 min post-irradiation and afterwards decreased over time reaching the pretreatment level 24 h after irradiation. Irradiated FA fibroblasts progressed to abnormal mitosis, as is shown by the production of cells with different nuclear morphologies from binucleated to multinucleated surrounded with micronuclei, and also by a high percentage of foci-positive micronuclei. The majority of radiation-induced micronuclei were gamma-H2AX foci-positive, indicating that radiation-induced micronuclei contain fragments of damaged chromosomes. In contrast, in the control group, most of the micronuclei were classified as gamma-H2AX foci-negative, which indicates that cells with unrepaired damage were blocked before entering mitosis. Conclusion: The results clearly indicate that mitotic catastrophe might be an important cell-death mechanism involved in the response of FA fibroblasts to ionizing radiation.",
journal = "International Journal of Radiation Biology",
title = "Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts",
volume = "90",
number = "5",
pages = "373-381",
doi = "10.3109/09553002.2014.892224"
}
Leskovac, A., Petrović, S., Guć-Šćekić, M., Vujic, D.,& Joksić, G.. (2014). Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts. in International Journal of Radiation Biology, 90(5), 373-381.
https://doi.org/10.3109/09553002.2014.892224
Leskovac A, Petrović S, Guć-Šćekić M, Vujic D, Joksić G. Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts. in International Journal of Radiation Biology. 2014;90(5):373-381.
doi:10.3109/09553002.2014.892224 .
Leskovac, Andreja, Petrović, Sandra, Guć-Šćekić, Marija, Vujic, Dragana, Joksić, Gordana, "Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts" in International Journal of Radiation Biology, 90, no. 5 (2014):373-381,
https://doi.org/10.3109/09553002.2014.892224 . .
1
4
4
4

Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials

Pagano, Giovanni; Talamanca, Annarita Aiello; Castello, Giuseppe; Cordero, Mario D.; d'Ischia, Marco; Gadaleta, Maria Nicola; Pallardo, Federico V.; Petrović, Sandra; Tiano, Luca; Zatterale, Adriana

(2014)

TY  - JOUR
AU  - Pagano, Giovanni
AU  - Talamanca, Annarita Aiello
AU  - Castello, Giuseppe
AU  - Cordero, Mario D.
AU  - d'Ischia, Marco
AU  - Gadaleta, Maria Nicola
AU  - Pallardo, Federico V.
AU  - Petrović, Sandra
AU  - Tiano, Luca
AU  - Zatterale, Adriana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/60
AB  - An extensive number of pathologies are associated with mitochondrial dysfunction (MDF) and oxidative stress (OS). Thus, mitochondrial cofactors termed mitochondrial nutrients (MN), such as alpha-lipoic acid (ALA), Coenzyme Q10 (CoQ10), and L-carnitine (CARN) (or its derivatives) have been tested in a number of clinical trials, and this review is focused on the use of MN-based clinical trials. The papers reporting on MN-based clinical trials were retrieved in MedLine up to July 2014, and evaluated for the following endpoints: (a) treated diseases; (b) dosages, number of enrolled patients and duration of treatment; (c) trial success for each MN or MN combinations as reported by authors. The reports satisfying the above endpoints included total numbers of trials and frequencies of randomized, controlled studies, i.e., 81 trials testing ALA, 107 reports testing CoQ10, and 74 reports testing CARN, while only 7 reports were retrieved testing double MN associations, while no report was found testing a triple MN combination. A total of 28 reports tested MN associations with classical antioxidants, such as antioxidant nutrients or drugs. Combinations of MN showed better outcomes than individual MN, suggesting forthcoming clinical studies. The criteria in study design and monitoring MN-based clinical trials are discussed.
T2  - International Journal of Molecular Sciences
T1  - Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials
VL  - 15
IS  - 11
SP  - 20169
EP  - 20208
DO  - 10.3390/ijms151120169
ER  - 
@article{
author = "Pagano, Giovanni and Talamanca, Annarita Aiello and Castello, Giuseppe and Cordero, Mario D. and d'Ischia, Marco and Gadaleta, Maria Nicola and Pallardo, Federico V. and Petrović, Sandra and Tiano, Luca and Zatterale, Adriana",
year = "2014",
abstract = "An extensive number of pathologies are associated with mitochondrial dysfunction (MDF) and oxidative stress (OS). Thus, mitochondrial cofactors termed mitochondrial nutrients (MN), such as alpha-lipoic acid (ALA), Coenzyme Q10 (CoQ10), and L-carnitine (CARN) (or its derivatives) have been tested in a number of clinical trials, and this review is focused on the use of MN-based clinical trials. The papers reporting on MN-based clinical trials were retrieved in MedLine up to July 2014, and evaluated for the following endpoints: (a) treated diseases; (b) dosages, number of enrolled patients and duration of treatment; (c) trial success for each MN or MN combinations as reported by authors. The reports satisfying the above endpoints included total numbers of trials and frequencies of randomized, controlled studies, i.e., 81 trials testing ALA, 107 reports testing CoQ10, and 74 reports testing CARN, while only 7 reports were retrieved testing double MN associations, while no report was found testing a triple MN combination. A total of 28 reports tested MN associations with classical antioxidants, such as antioxidant nutrients or drugs. Combinations of MN showed better outcomes than individual MN, suggesting forthcoming clinical studies. The criteria in study design and monitoring MN-based clinical trials are discussed.",
journal = "International Journal of Molecular Sciences",
title = "Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials",
volume = "15",
number = "11",
pages = "20169-20208",
doi = "10.3390/ijms151120169"
}
Pagano, G., Talamanca, A. A., Castello, G., Cordero, M. D., d'Ischia, M., Gadaleta, M. N., Pallardo, F. V., Petrović, S., Tiano, L.,& Zatterale, A.. (2014). Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials. in International Journal of Molecular Sciences, 15(11), 20169-20208.
https://doi.org/10.3390/ijms151120169
Pagano G, Talamanca AA, Castello G, Cordero MD, d'Ischia M, Gadaleta MN, Pallardo FV, Petrović S, Tiano L, Zatterale A. Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials. in International Journal of Molecular Sciences. 2014;15(11):20169-20208.
doi:10.3390/ijms151120169 .
Pagano, Giovanni, Talamanca, Annarita Aiello, Castello, Giuseppe, Cordero, Mario D., d'Ischia, Marco, Gadaleta, Maria Nicola, Pallardo, Federico V., Petrović, Sandra, Tiano, Luca, Zatterale, Adriana, "Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials" in International Journal of Molecular Sciences, 15, no. 11 (2014):20169-20208,
https://doi.org/10.3390/ijms151120169 . .
18
15
17

Oxidative Stress and Mitochondrial Dysfunction across Broad-Ranging Pathologies: Toward Mitochondria-Targeted Clinical Strategies

Pagano, Giovanni; Talamanca, Annarita Aiello; Castello, Giuseppe; Cordero, Mario D.; d'Ischia, Marco; Gadaleta, Maria Nicola; Pallardo, Federico V.; Petrović, Sandra; Tiano, Luca; Zatterale, Adriana

(2014)

TY  - JOUR
AU  - Pagano, Giovanni
AU  - Talamanca, Annarita Aiello
AU  - Castello, Giuseppe
AU  - Cordero, Mario D.
AU  - d'Ischia, Marco
AU  - Gadaleta, Maria Nicola
AU  - Pallardo, Federico V.
AU  - Petrović, Sandra
AU  - Tiano, Luca
AU  - Zatterale, Adriana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6007
AB  - Beyond the disorders recognized as mitochondrial diseases, abnormalities in function and/or ultrastructure of mitochondria have been reported in several unrelated pathologies. These encompass ageing, malformations, and a number of genetic or acquired diseases, as diabetes and cardiologic, haematologic, organ-specific (e.g., eye or liver), neurologic and psychiatric, autoimmune, and dermatologic disorders. The mechanistic grounds for mitochondrial dysfunction (MDF) along with the occurrence of oxidative stress (OS) have been investigated within the pathogenesis of individual disorders or in groups of interrelated disorders. We attempt to review broad-ranging pathologies that involvemitochondrial-specific deficiencies or rely on cytosol-derived prooxidant states or on autoimmune-induced mitochondrial damage. The established knowledge in these subjects warrants studies aimed at elucidating several open questions that are highlighted in the present review. The relevance of OS and MDF in different pathologies may establish the grounds for chemoprevention trials aimed at compensating OS/MDF by means of antioxidants and mitochondrial nutrients.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Oxidative Stress and Mitochondrial Dysfunction across Broad-Ranging Pathologies: Toward Mitochondria-Targeted Clinical Strategies
DO  - 10.1155/2014/541230
ER  - 
@article{
author = "Pagano, Giovanni and Talamanca, Annarita Aiello and Castello, Giuseppe and Cordero, Mario D. and d'Ischia, Marco and Gadaleta, Maria Nicola and Pallardo, Federico V. and Petrović, Sandra and Tiano, Luca and Zatterale, Adriana",
year = "2014",
abstract = "Beyond the disorders recognized as mitochondrial diseases, abnormalities in function and/or ultrastructure of mitochondria have been reported in several unrelated pathologies. These encompass ageing, malformations, and a number of genetic or acquired diseases, as diabetes and cardiologic, haematologic, organ-specific (e.g., eye or liver), neurologic and psychiatric, autoimmune, and dermatologic disorders. The mechanistic grounds for mitochondrial dysfunction (MDF) along with the occurrence of oxidative stress (OS) have been investigated within the pathogenesis of individual disorders or in groups of interrelated disorders. We attempt to review broad-ranging pathologies that involvemitochondrial-specific deficiencies or rely on cytosol-derived prooxidant states or on autoimmune-induced mitochondrial damage. The established knowledge in these subjects warrants studies aimed at elucidating several open questions that are highlighted in the present review. The relevance of OS and MDF in different pathologies may establish the grounds for chemoprevention trials aimed at compensating OS/MDF by means of antioxidants and mitochondrial nutrients.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Oxidative Stress and Mitochondrial Dysfunction across Broad-Ranging Pathologies: Toward Mitochondria-Targeted Clinical Strategies",
doi = "10.1155/2014/541230"
}
Pagano, G., Talamanca, A. A., Castello, G., Cordero, M. D., d'Ischia, M., Gadaleta, M. N., Pallardo, F. V., Petrović, S., Tiano, L.,& Zatterale, A.. (2014). Oxidative Stress and Mitochondrial Dysfunction across Broad-Ranging Pathologies: Toward Mitochondria-Targeted Clinical Strategies. in Oxidative Medicine and Cellular Longevity.
https://doi.org/10.1155/2014/541230
Pagano G, Talamanca AA, Castello G, Cordero MD, d'Ischia M, Gadaleta MN, Pallardo FV, Petrović S, Tiano L, Zatterale A. Oxidative Stress and Mitochondrial Dysfunction across Broad-Ranging Pathologies: Toward Mitochondria-Targeted Clinical Strategies. in Oxidative Medicine and Cellular Longevity. 2014;.
doi:10.1155/2014/541230 .
Pagano, Giovanni, Talamanca, Annarita Aiello, Castello, Giuseppe, Cordero, Mario D., d'Ischia, Marco, Gadaleta, Maria Nicola, Pallardo, Federico V., Petrović, Sandra, Tiano, Luca, Zatterale, Adriana, "Oxidative Stress and Mitochondrial Dysfunction across Broad-Ranging Pathologies: Toward Mitochondria-Targeted Clinical Strategies" in Oxidative Medicine and Cellular Longevity (2014),
https://doi.org/10.1155/2014/541230 . .
2
97
76
90

The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin

Leskovac, Andreja; Joksić, Gordana; Pašti, Igor A.; Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Petrović, Sandra

(2013)

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Joksić, Gordana
AU  - Pašti, Igor A.
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Petrović, Sandra
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5830
AB  - Herbal preparation Chlamyfin was investigated for its total polyphenol content, 2,2-diphenyl-1picrylhydrazyl (DPPH) scavenging activity, and anti-inflammatory and genotoxic properties. A high total polyphenol content provided evidence of high DPPH radical scavenging activity (IC50 = 4.96 +/- 0.23 mu g/ml). Analysis of the electrochemical behavior of Chlamyfin indicated high reducing ability, i.e., high antioxidant capacity, in agreement with the DPPH test. Analysis of myeloperoxidase (MPO) inhibition by Chlamyfin suggested anti-inflammatory action (IC50 values of 5.40 mu g/ml and 4.45 i_tg/m1 for an incubation time of 10 and 30 min, respectively). For genotoxic assessment, oxidative stress was induced by irradiation of peripheral whole blood with gamma-radiation in vitro. In the presence of Chlamyfin, reduced incidence of micronuclei without disturbance to the proliferative potential of cells was evidenced in both irradiated and unirradiated samples, indicating its genoprotective properties. It was shown that Chlamyfm, in addition to its bactericidal effect, also possesses strong antioxidant, anti-inflammatory and anti-genotoxic properties.
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin
VL  - 32
IS  - 2
SP  - 227
EP  - 237
ER  - 
@article{
author = "Leskovac, Andreja and Joksić, Gordana and Pašti, Igor A. and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Petrović, Sandra",
year = "2013",
abstract = "Herbal preparation Chlamyfin was investigated for its total polyphenol content, 2,2-diphenyl-1picrylhydrazyl (DPPH) scavenging activity, and anti-inflammatory and genotoxic properties. A high total polyphenol content provided evidence of high DPPH radical scavenging activity (IC50 = 4.96 +/- 0.23 mu g/ml). Analysis of the electrochemical behavior of Chlamyfin indicated high reducing ability, i.e., high antioxidant capacity, in agreement with the DPPH test. Analysis of myeloperoxidase (MPO) inhibition by Chlamyfin suggested anti-inflammatory action (IC50 values of 5.40 mu g/ml and 4.45 i_tg/m1 for an incubation time of 10 and 30 min, respectively). For genotoxic assessment, oxidative stress was induced by irradiation of peripheral whole blood with gamma-radiation in vitro. In the presence of Chlamyfin, reduced incidence of micronuclei without disturbance to the proliferative potential of cells was evidenced in both irradiated and unirradiated samples, indicating its genoprotective properties. It was shown that Chlamyfm, in addition to its bactericidal effect, also possesses strong antioxidant, anti-inflammatory and anti-genotoxic properties.",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin",
volume = "32",
number = "2",
pages = "227-237"
}
Leskovac, A., Joksić, G., Pašti, I. A., Lazarević-Pašti, T., Nastasijević, B. J.,& Petrović, S.. (2013). The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin. in Macedonian Journal of Chemistry and Chemical Engineering, 32(2), 227-237.
Leskovac A, Joksić G, Pašti IA, Lazarević-Pašti T, Nastasijević BJ, Petrović S. The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin. in Macedonian Journal of Chemistry and Chemical Engineering. 2013;32(2):227-237..
Leskovac, Andreja, Joksić, Gordana, Pašti, Igor A., Lazarević-Pašti, Tamara, Nastasijević, Branislav J., Petrović, Sandra, "The Antiradical, Anti-Inflammatory and Anti-Genotoxic Potential of Herbal Preparation Chlamyfin" in Macedonian Journal of Chemistry and Chemical Engineering, 32, no. 2 (2013):227-237.
5

In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation

Petrovic, Voin; Čolović, Mirjana B.; Krstić, Danijela Z.; Vujačić, Ana V.; Petrović, Sandra; Joksić, Gordana; Bugarčić, Živadin D.; Vasić, Vesna M.

(2013)

TY  - JOUR
AU  - Petrovic, Voin
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Vujačić, Ana V.
AU  - Petrović, Sandra
AU  - Joksić, Gordana
AU  - Bugarčić, Živadin D.
AU  - Vasić, Vesna M.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5539
AB  - The in vitro influence of gold(III) complexes, H[AuCl4], [Au(DMSO)(2)Cl-2]Cl and [Au(bipy)Cl-2]Cl (bipy = 2,2-bipyridine), upon commercially available Na+ /K+ ATPase activity, purified from porcine brain cortex, was investigated. Additionally, the complexes were tested on human lymphocytes, and incidence of micronuclei and cell proliferation index was determined. Concentration-dependent inhibition of the enzyme for all three compounds was obtained, but with differing potencies. Calculated IC50 from Hill analysis were (in M): 5.75 x 10(-7), 5.50 x 10(-6) and 3.98 x 10(-5), for H[AuCl4], [Au(DMSO)(2)Cl-2]Cl and [Au(bipy)Cl-2]Cl, respectively, while Hill coefficient values, n, were above 1 in all cases. This inhibition can be prevented using -SH donating ligands such as L-Cys and glutathione, and these ligands can also cause a recovery of the enzyme activity after the induced inhibition. Kinetic analysis demonstrated that each of the studied gold(III) complexes affects Na+ /K+ ATPase reducing maximum enzymatic velocity, V-max, but not significantly changing the affinity for the substrate (K-M value), implying a noncompetitive mode of the interaction. Furthermore, among investigated gold(III) complexes, the [Au(bipy)Cl-2]Cl complex exhibits a strong cytotoxic effect on human lymphocytes, which suggests its potential for use in antitumor therapy. (C(C) 2013 Elsevier Inc. All rights reserved.
T2  - Journal of Inorganic Biochemistry
T1  - In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation
VL  - 124
SP  - 35
EP  - 41
DO  - 10.1016/j.jinorgbio.2013.03.013
ER  - 
@article{
author = "Petrovic, Voin and Čolović, Mirjana B. and Krstić, Danijela Z. and Vujačić, Ana V. and Petrović, Sandra and Joksić, Gordana and Bugarčić, Živadin D. and Vasić, Vesna M.",
year = "2013",
abstract = "The in vitro influence of gold(III) complexes, H[AuCl4], [Au(DMSO)(2)Cl-2]Cl and [Au(bipy)Cl-2]Cl (bipy = 2,2-bipyridine), upon commercially available Na+ /K+ ATPase activity, purified from porcine brain cortex, was investigated. Additionally, the complexes were tested on human lymphocytes, and incidence of micronuclei and cell proliferation index was determined. Concentration-dependent inhibition of the enzyme for all three compounds was obtained, but with differing potencies. Calculated IC50 from Hill analysis were (in M): 5.75 x 10(-7), 5.50 x 10(-6) and 3.98 x 10(-5), for H[AuCl4], [Au(DMSO)(2)Cl-2]Cl and [Au(bipy)Cl-2]Cl, respectively, while Hill coefficient values, n, were above 1 in all cases. This inhibition can be prevented using -SH donating ligands such as L-Cys and glutathione, and these ligands can also cause a recovery of the enzyme activity after the induced inhibition. Kinetic analysis demonstrated that each of the studied gold(III) complexes affects Na+ /K+ ATPase reducing maximum enzymatic velocity, V-max, but not significantly changing the affinity for the substrate (K-M value), implying a noncompetitive mode of the interaction. Furthermore, among investigated gold(III) complexes, the [Au(bipy)Cl-2]Cl complex exhibits a strong cytotoxic effect on human lymphocytes, which suggests its potential for use in antitumor therapy. (C(C) 2013 Elsevier Inc. All rights reserved.",
journal = "Journal of Inorganic Biochemistry",
title = "In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation",
volume = "124",
pages = "35-41",
doi = "10.1016/j.jinorgbio.2013.03.013"
}
Petrovic, V., Čolović, M. B., Krstić, D. Z., Vujačić, A. V., Petrović, S., Joksić, G., Bugarčić, Ž. D.,& Vasić, V. M.. (2013). In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation. in Journal of Inorganic Biochemistry, 124, 35-41.
https://doi.org/10.1016/j.jinorgbio.2013.03.013
Petrovic V, Čolović MB, Krstić DZ, Vujačić AV, Petrović S, Joksić G, Bugarčić ŽD, Vasić VM. In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation. in Journal of Inorganic Biochemistry. 2013;124:35-41.
doi:10.1016/j.jinorgbio.2013.03.013 .
Petrovic, Voin, Čolović, Mirjana B., Krstić, Danijela Z., Vujačić, Ana V., Petrović, Sandra, Joksić, Gordana, Bugarčić, Živadin D., Vasić, Vesna M., "In vitro effects of some gold complexes on Na+/K+ ATPase activity and cell proliferation" in Journal of Inorganic Biochemistry, 124 (2013):35-41,
https://doi.org/10.1016/j.jinorgbio.2013.03.013 . .
13
13
12

Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia

Petrović, Sandra; Leskovac, Andreja; Joksić, Ivana; Filipović, Jelena G.; Vujic, Dragana; Guć-Šćekić, Marija; Joksić, Gordana

(2013)

TY  - JOUR
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Filipović, Jelena G.
AU  - Vujic, Dragana
AU  - Guć-Šćekić, Marija
AU  - Joksić, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5606
AB  - Fanconi anemia (FA) is a rare cancer-prone genetic disease characterized by impaired oxygen metabolism and defects in DNA damage repair. Response of FA cells to ionizing radiation has been an issue intensively debated in the literature. To study in vitro radiosensitivity in patients suffering from FA and their parents (heterozygous carriers), we determined radiation-induced leukocyte apoptosis using flow cytometry. As TP53 gene is involved in the control of apoptosis, we studied its status in FA lymphocytes using dual colour fluorescence in situ hybridization (FISH). FA patients and female heterozygous carriers display radiosensitive response to ionizing radiation seen as abnormal elimination of cells via apoptosis. By employment of FISH, the TP53 allele loss in FA lymphocytes was not observed. In diseases related to oxidative stress, determination of radiation-induced apoptosis is the method of choice for testing the radiosensitivity.
T2  - Current Science
T1  - Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia
VL  - 105
IS  - 1
SP  - 56
EP  - 60
ER  - 
@article{
author = "Petrović, Sandra and Leskovac, Andreja and Joksić, Ivana and Filipović, Jelena G. and Vujic, Dragana and Guć-Šćekić, Marija and Joksić, Gordana",
year = "2013",
abstract = "Fanconi anemia (FA) is a rare cancer-prone genetic disease characterized by impaired oxygen metabolism and defects in DNA damage repair. Response of FA cells to ionizing radiation has been an issue intensively debated in the literature. To study in vitro radiosensitivity in patients suffering from FA and their parents (heterozygous carriers), we determined radiation-induced leukocyte apoptosis using flow cytometry. As TP53 gene is involved in the control of apoptosis, we studied its status in FA lymphocytes using dual colour fluorescence in situ hybridization (FISH). FA patients and female heterozygous carriers display radiosensitive response to ionizing radiation seen as abnormal elimination of cells via apoptosis. By employment of FISH, the TP53 allele loss in FA lymphocytes was not observed. In diseases related to oxidative stress, determination of radiation-induced apoptosis is the method of choice for testing the radiosensitivity.",
journal = "Current Science",
title = "Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia",
volume = "105",
number = "1",
pages = "56-60"
}
Petrović, S., Leskovac, A., Joksić, I., Filipović, J. G., Vujic, D., Guć-Šćekić, M.,& Joksić, G.. (2013). Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia. in Current Science, 105(1), 56-60.
Petrović S, Leskovac A, Joksić I, Filipović JG, Vujic D, Guć-Šćekić M, Joksić G. Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia. in Current Science. 2013;105(1):56-60..
Petrović, Sandra, Leskovac, Andreja, Joksić, Ivana, Filipović, Jelena G., Vujic, Dragana, Guć-Šćekić, Marija, Joksić, Gordana, "Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia" in Current Science, 105, no. 1 (2013):56-60.
1

Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype

Joksić, Ivana; Petrović, Sandra; Leskovac, Andreja; Filipović, Jelena G.; Guć-Šćekić, Marija; Vujic, Dragana; Joksić, Gordana

(2013)

TY  - JOUR
AU  - Joksić, Ivana
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Filipović, Jelena G.
AU  - Guć-Šćekić, Marija
AU  - Vujic, Dragana
AU  - Joksić, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5798
AB  - Fanconi anemia (FA) is a rare genetically heterogeneous disease characterized by developmental abnormalities, progressive bone marrow failure, and cancer susceptibility. We examined spontaneous, diepoxybutane (DEB)-induced and radiation-induced sister chromatid exchanges (SCEs) in wholeblood lymphocyte cultures of bone marrow failure (BMF) patients including Fanconi anemia, mothers of affected individuals, and healthy controls. The baseline frequency of SCE in FA cells was similar to that observed in controls. However, in response to DEB SCE frequencies in FA patients and their mothers were significantly increased compared to both non-FA BMF families and healthy controls. In response to ionizing radiation, cells displayed increased frequency of SCE, but no differences between FA patients and non-FA BMF patients were seen. Our data confirm and expand previous findings by showing that SCE induced by DEB can be used as an adjunct diagnostic test not only for FA patients, but also for female heterozygous carriers, at least for complementation groups FANCA and FANCD2.
T2  - Genetika (Beograd)
T1  - Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype
VL  - 45
IS  - 2
SP  - 393
EP  - 403
DO  - 10.2298/GENSR1302393J
ER  - 
@article{
author = "Joksić, Ivana and Petrović, Sandra and Leskovac, Andreja and Filipović, Jelena G. and Guć-Šćekić, Marija and Vujic, Dragana and Joksić, Gordana",
year = "2013",
abstract = "Fanconi anemia (FA) is a rare genetically heterogeneous disease characterized by developmental abnormalities, progressive bone marrow failure, and cancer susceptibility. We examined spontaneous, diepoxybutane (DEB)-induced and radiation-induced sister chromatid exchanges (SCEs) in wholeblood lymphocyte cultures of bone marrow failure (BMF) patients including Fanconi anemia, mothers of affected individuals, and healthy controls. The baseline frequency of SCE in FA cells was similar to that observed in controls. However, in response to DEB SCE frequencies in FA patients and their mothers were significantly increased compared to both non-FA BMF families and healthy controls. In response to ionizing radiation, cells displayed increased frequency of SCE, but no differences between FA patients and non-FA BMF patients were seen. Our data confirm and expand previous findings by showing that SCE induced by DEB can be used as an adjunct diagnostic test not only for FA patients, but also for female heterozygous carriers, at least for complementation groups FANCA and FANCD2.",
journal = "Genetika (Beograd)",
title = "Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype",
volume = "45",
number = "2",
pages = "393-403",
doi = "10.2298/GENSR1302393J"
}
Joksić, I., Petrović, S., Leskovac, A., Filipović, J. G., Guć-Šćekić, M., Vujic, D.,& Joksić, G.. (2013). Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype. in Genetika (Beograd), 45(2), 393-403.
https://doi.org/10.2298/GENSR1302393J
Joksić I, Petrović S, Leskovac A, Filipović JG, Guć-Šćekić M, Vujic D, Joksić G. Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype. in Genetika (Beograd). 2013;45(2):393-403.
doi:10.2298/GENSR1302393J .
Joksić, Ivana, Petrović, Sandra, Leskovac, Andreja, Filipović, Jelena G., Guć-Šćekić, Marija, Vujic, Dragana, Joksić, Gordana, "Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype" in Genetika (Beograd), 45, no. 2 (2013):393-403,
https://doi.org/10.2298/GENSR1302393J . .
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2

From clinical description, to in vitro and animal studies, and backward to patients: Oxidative stress and mitochondrial dysfunction in Fanconi anemia

Pagano, Giovanni; Talamanca, Annarita Aiello; Castello, Giuseppe; d'Ischia, Marco; Pallardo, Federico V.; Petrović, Sandra; Porto, Beatriz; Tiano, Luca; Zatterale, Adriana

(2013)

TY  - JOUR
AU  - Pagano, Giovanni
AU  - Talamanca, Annarita Aiello
AU  - Castello, Giuseppe
AU  - d'Ischia, Marco
AU  - Pallardo, Federico V.
AU  - Petrović, Sandra
AU  - Porto, Beatriz
AU  - Tiano, Luca
AU  - Zatterale, Adriana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5454
AB  - Fanconi anemia (FA) is a rare genetic disease associated with deficiencies in DNA repair pathways. A body of literature points to a pro-oxidant state in FA patients, along with evidence for oxidative stress (OS) in the FA phenotype reported by in vitro, molecular, and animal studies. A highlight arises from the detection of mitochondrial dysfunction (MDF) in FA cell lines of complementation groups A, C, D2, and G. As yet lacking, in vivo studies should focus on FA-associated MDF, which may help in the understanding of the mitochondrial basis of OS detected in cells and body fluids from FA patients. Beyond the in vitro and animal databases, the available analytical devices may prompt the direct observation of metabolic and mitochondrial alterations in FA patients. These studies should evaluate a set of MDF-related endpoints, to be related to OS endpoints. The working hypothesis is raised that, parallel to OS, nitrosative stress might be another, so far unexplored, hallmark of the FA phenotype. The expected results may shed light on the FA pathogenesis and might provide grounds for pilot chemoprevention trials using mitochondrial nutrients. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Free Radical Biology and Medicine
T1  - From clinical description, to in vitro and animal studies, and backward to patients: Oxidative stress and mitochondrial dysfunction in Fanconi anemia
VL  - 58
SP  - 118
EP  - 125
DO  - 10.1016/j.freeradbiomed.2013.01.015
ER  - 
@article{
author = "Pagano, Giovanni and Talamanca, Annarita Aiello and Castello, Giuseppe and d'Ischia, Marco and Pallardo, Federico V. and Petrović, Sandra and Porto, Beatriz and Tiano, Luca and Zatterale, Adriana",
year = "2013",
abstract = "Fanconi anemia (FA) is a rare genetic disease associated with deficiencies in DNA repair pathways. A body of literature points to a pro-oxidant state in FA patients, along with evidence for oxidative stress (OS) in the FA phenotype reported by in vitro, molecular, and animal studies. A highlight arises from the detection of mitochondrial dysfunction (MDF) in FA cell lines of complementation groups A, C, D2, and G. As yet lacking, in vivo studies should focus on FA-associated MDF, which may help in the understanding of the mitochondrial basis of OS detected in cells and body fluids from FA patients. Beyond the in vitro and animal databases, the available analytical devices may prompt the direct observation of metabolic and mitochondrial alterations in FA patients. These studies should evaluate a set of MDF-related endpoints, to be related to OS endpoints. The working hypothesis is raised that, parallel to OS, nitrosative stress might be another, so far unexplored, hallmark of the FA phenotype. The expected results may shed light on the FA pathogenesis and might provide grounds for pilot chemoprevention trials using mitochondrial nutrients. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Free Radical Biology and Medicine",
title = "From clinical description, to in vitro and animal studies, and backward to patients: Oxidative stress and mitochondrial dysfunction in Fanconi anemia",
volume = "58",
pages = "118-125",
doi = "10.1016/j.freeradbiomed.2013.01.015"
}
Pagano, G., Talamanca, A. A., Castello, G., d'Ischia, M., Pallardo, F. V., Petrović, S., Porto, B., Tiano, L.,& Zatterale, A.. (2013). From clinical description, to in vitro and animal studies, and backward to patients: Oxidative stress and mitochondrial dysfunction in Fanconi anemia. in Free Radical Biology and Medicine, 58, 118-125.
https://doi.org/10.1016/j.freeradbiomed.2013.01.015
Pagano G, Talamanca AA, Castello G, d'Ischia M, Pallardo FV, Petrović S, Porto B, Tiano L, Zatterale A. From clinical description, to in vitro and animal studies, and backward to patients: Oxidative stress and mitochondrial dysfunction in Fanconi anemia. in Free Radical Biology and Medicine. 2013;58:118-125.
doi:10.1016/j.freeradbiomed.2013.01.015 .
Pagano, Giovanni, Talamanca, Annarita Aiello, Castello, Giuseppe, d'Ischia, Marco, Pallardo, Federico V., Petrović, Sandra, Porto, Beatriz, Tiano, Luca, Zatterale, Adriana, "From clinical description, to in vitro and animal studies, and backward to patients: Oxidative stress and mitochondrial dysfunction in Fanconi anemia" in Free Radical Biology and Medicine, 58 (2013):118-125,
https://doi.org/10.1016/j.freeradbiomed.2013.01.015 . .
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Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing

Vujic, Dragana S.; Petrović, Sandra; Leskovac, Andreja; Joksić, Ivana; Filipović, Jelena G.; Valenta-Šobot, Ana

(2013)

TY  - JOUR
AU  - Vujic, Dragana S.
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Filipović, Jelena G.
AU  - Valenta-Šobot, Ana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5595
AB  - In this paper we present the data of lymphocyte radiosensitivity testing used for characterization of radiosensitive cellular phenotype and diagnostics of ataxia-telangiectasia disease. We point out the advantage of lymphocyte micronucleus test (CBMN) over other cellular tests for assessment of radiosensitivity: the first advantage of CBMN is that primary patient cells are used (less than 1 ml), the second one is that the results of testing are obtained within 3 days and there is no need for establishing a patient-derived cell line, which requires additional time and application of more expensive methods. The third advantage of CBMN method is that it gives information about proliferative ability of cells, which can recognize dysfunctional ataxia-telangiectasia mutated protein. The results are fast and accurate in diagnostics of ataxia-telagiectasia diseases.
T2  - Nuclear technology and radiation protection
T1  - Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing
VL  - 28
IS  - 2
SP  - 221
EP  - 224
DO  - 10.2298/NTRP1302221V
ER  - 
@article{
author = "Vujic, Dragana S. and Petrović, Sandra and Leskovac, Andreja and Joksić, Ivana and Filipović, Jelena G. and Valenta-Šobot, Ana",
year = "2013",
abstract = "In this paper we present the data of lymphocyte radiosensitivity testing used for characterization of radiosensitive cellular phenotype and diagnostics of ataxia-telangiectasia disease. We point out the advantage of lymphocyte micronucleus test (CBMN) over other cellular tests for assessment of radiosensitivity: the first advantage of CBMN is that primary patient cells are used (less than 1 ml), the second one is that the results of testing are obtained within 3 days and there is no need for establishing a patient-derived cell line, which requires additional time and application of more expensive methods. The third advantage of CBMN method is that it gives information about proliferative ability of cells, which can recognize dysfunctional ataxia-telangiectasia mutated protein. The results are fast and accurate in diagnostics of ataxia-telagiectasia diseases.",
journal = "Nuclear technology and radiation protection",
title = "Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing",
volume = "28",
number = "2",
pages = "221-224",
doi = "10.2298/NTRP1302221V"
}
Vujic, D. S., Petrović, S., Leskovac, A., Joksić, I., Filipović, J. G.,& Valenta-Šobot, A.. (2013). Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing. in Nuclear technology and radiation protection, 28(2), 221-224.
https://doi.org/10.2298/NTRP1302221V
Vujic DS, Petrović S, Leskovac A, Joksić I, Filipović JG, Valenta-Šobot A. Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing. in Nuclear technology and radiation protection. 2013;28(2):221-224.
doi:10.2298/NTRP1302221V .
Vujic, Dragana S., Petrović, Sandra, Leskovac, Andreja, Joksić, Ivana, Filipović, Jelena G., Valenta-Šobot, Ana, "Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing" in Nuclear technology and radiation protection, 28, no. 2 (2013):221-224,
https://doi.org/10.2298/NTRP1302221V . .
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