TY  - JOUR
AU  - Stefanović, Milan
AU  - Stojković, Ljiljana
AU  - Životić, Ivan
AU  - Dinčić, Evica
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12715
AB  - Multiple sclerosis (MS), a noncurable autoimmune neurodegenerative disease, requires constant research that could improve understanding of both environmental and genetic factors that lead to its occurrence and/or progression. Recognition of the genetic basis of MS further leads to an investigation of the regulatory role of genetic variants on gene expression. Among risk variants for MS, Ikaros zinc finger 3 (IKZF3) gene variant rs12946510 was identified as one of the top-ranked and the expression quantitative trait loci (eQTL) for genes residing in chromosomal locus 17q12- 21. The study aimed to investigate the association of gene expression of the immunologically relevant genes, which map to indicated locus, ORMDL3, GSDMB, and IKZF3, with MS and rs12946510 genotype, taking into account disease phase, clinical parameters of disease progression, and severity and immunomodulatory therapy. We used TaqMan® technology for both allelic discrimination and gene expression determination in 67 relapsing MS patients and 50 healthy controls. Decreased ORMDL3 and GSDMB mRNA levels had significant associations with MS and rs12946510 TT rare homozygote among patients. Significant positive correlations between ORMDL3 and GSDMB mRNA expression were observed in both patients and controls. We detected the significant between-effect of sex and rs12946510 on the expression of ORMDL3 in the patient group and interferon β therapy and rs12946510 on GSDMB expression. Our results show the association of ORMDL3 and GSDMB mRNA expression with the clinical manifestation of MS and confirm that IKZF3 rs12946510 exerts the eQTL effect on both genes in multiple sclerosis. Besides providing novel insight related to MS phases and interferon β therapy, the study results confirm previous studies on regulatory genetic variants, autoimmunity, and MS.
T2  - Heliyon
T1  - Expression levels of GSDMB and ORMDL3 are associated with relapsing-remitting multiple sclerosis and IKZF3 rs12946510 variant
VL  - 10
IS  - 3
SP  - e25033
DO  - 10.1016/j.heliyon.2024.e25033
ER  - 

@article{
author = "Stefanović, Milan and Stojković, Ljiljana and Životić, Ivan and Dinčić, Evica and Stanković, Aleksandra and Živković, Maja",
year = "2024",
abstract = "Multiple sclerosis (MS), a noncurable autoimmune neurodegenerative disease, requires constant research that could improve understanding of both environmental and genetic factors that lead to its occurrence and/or progression. Recognition of the genetic basis of MS further leads to an investigation of the regulatory role of genetic variants on gene expression. Among risk variants for MS, Ikaros zinc finger 3 (IKZF3) gene variant rs12946510 was identified as one of the top-ranked and the expression quantitative trait loci (eQTL) for genes residing in chromosomal locus 17q12- 21. The study aimed to investigate the association of gene expression of the immunologically relevant genes, which map to indicated locus, ORMDL3, GSDMB, and IKZF3, with MS and rs12946510 genotype, taking into account disease phase, clinical parameters of disease progression, and severity and immunomodulatory therapy. We used TaqMan® technology for both allelic discrimination and gene expression determination in 67 relapsing MS patients and 50 healthy controls. Decreased ORMDL3 and GSDMB mRNA levels had significant associations with MS and rs12946510 TT rare homozygote among patients. Significant positive correlations between ORMDL3 and GSDMB mRNA expression were observed in both patients and controls. We detected the significant between-effect of sex and rs12946510 on the expression of ORMDL3 in the patient group and interferon β therapy and rs12946510 on GSDMB expression. Our results show the association of ORMDL3 and GSDMB mRNA expression with the clinical manifestation of MS and confirm that IKZF3 rs12946510 exerts the eQTL effect on both genes in multiple sclerosis. Besides providing novel insight related to MS phases and interferon β therapy, the study results confirm previous studies on regulatory genetic variants, autoimmunity, and MS.",
journal = "Heliyon",
title = "Expression levels of GSDMB and ORMDL3 are associated with relapsing-remitting multiple sclerosis and IKZF3 rs12946510 variant",
volume = "10",
number = "3",
pages = "e25033",
doi = "10.1016/j.heliyon.2024.e25033"
}

Stefanović, M., Stojković, L., Životić, I., Dinčić, E., Stanković, A.,& Živković, M.. (2024). Expression levels of GSDMB and ORMDL3 are associated with relapsing-remitting multiple sclerosis and IKZF3 rs12946510 variant. in Heliyon, 10(3), e25033.
https://doi.org/10.1016/j.heliyon.2024.e25033

Stefanović M, Stojković L, Životić I, Dinčić E, Stanković A, Živković M. Expression levels of GSDMB and ORMDL3 are associated with relapsing-remitting multiple sclerosis and IKZF3 rs12946510 variant. in Heliyon. 2024;10(3):e25033.
doi:10.1016/j.heliyon.2024.e25033 .

Stefanović, Milan, Stojković, Ljiljana, Životić, Ivan, Dinčić, Evica, Stanković, Aleksandra, Živković, Maja, "Expression levels of GSDMB and ORMDL3 are associated with relapsing-remitting multiple sclerosis and IKZF3 rs12946510 variant" in Heliyon, 10, no. 3 (2024):e25033,
https://doi.org/10.1016/j.heliyon.2024.e25033 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Pavlović, Slađan
AU  - Dinčić, Evica
AU  - Kuveljić, Jovana
AU  - Manojlović, Dragan
AU  - Živković, Maja
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12937
AB  - Background  Multiple sclerosis (MS) is a chronic demyelinating disorder intricately linked to perturbations in trace element levels. While previous studies have explored circulating trace elements in a limited sample, understanding the impact of demographic and clinical variables on the elemental profile within a larger cohort remains elusive. Methods  This study aimed to evaluate essential trace elements (Cr, Mn, Co, Cu, Zn, and Se) in the sera of 215 MS patients compared to a meticulously matched control group of 100 individuals with similar gender and age. Our main objective was to identify potential variations in elemental profiles based on demographic and clinical parameters among MS patients, elucidating the prospective relevance of supplementing specific essential trace elements. Results  Data indicated a significant decrease in serum levels of Mn, Co, Zn, and Se, and an increase in Cr in MS patients compared to controls. These trace elements not only discriminated between MS patients and controls but also exhibited distinctive capabilities among demographic subgroups. Gender, smoking habits, and age strata (20-40 years and 41-60 years) revealed discernible variations in elemental profiles between MS patients and their control counterparts. Se demonstrated the singular ability to stratify cases of extreme MS severity, mild relapsing-remitting MS (RRMS) and highly severe secondary progressive MS (SPMS). In contrast, Co significantly differentiated RRMS from primary progressive MS (PPMS), while Cu significantly differentiated SPMS from PPMS. Additionally, Cu showed a negative correlation with MSSS, while Mn and Zn showed a positive correlation with EDSS. Conclusion  These findings underscore a substantive deficiency in Mn, Co, Zn, and Se in the MS cohort, supporting targeted supplementation with these trace elements. This study provides a comprehensive understanding of the intricate relationship between essential trace elements and MS, paving the way for further research into personalized nutritional interventions for this complex neurological disorder.
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Essential trace element levels in multiple sclerosis: bridging demographic and clinical gaps, assessing the need for supplementation
VL  - 83
SP  - 127421
DO  - 10.1016/j.jtemb.2024.127421
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Jagodić, Jovana and Pavlović, Slađan and Dinčić, Evica and Kuveljić, Jovana and Manojlović, Dragan and Živković, Maja",
year = "2024",
abstract = "Background  Multiple sclerosis (MS) is a chronic demyelinating disorder intricately linked to perturbations in trace element levels. While previous studies have explored circulating trace elements in a limited sample, understanding the impact of demographic and clinical variables on the elemental profile within a larger cohort remains elusive. Methods  This study aimed to evaluate essential trace elements (Cr, Mn, Co, Cu, Zn, and Se) in the sera of 215 MS patients compared to a meticulously matched control group of 100 individuals with similar gender and age. Our main objective was to identify potential variations in elemental profiles based on demographic and clinical parameters among MS patients, elucidating the prospective relevance of supplementing specific essential trace elements. Results  Data indicated a significant decrease in serum levels of Mn, Co, Zn, and Se, and an increase in Cr in MS patients compared to controls. These trace elements not only discriminated between MS patients and controls but also exhibited distinctive capabilities among demographic subgroups. Gender, smoking habits, and age strata (20-40 years and 41-60 years) revealed discernible variations in elemental profiles between MS patients and their control counterparts. Se demonstrated the singular ability to stratify cases of extreme MS severity, mild relapsing-remitting MS (RRMS) and highly severe secondary progressive MS (SPMS). In contrast, Co significantly differentiated RRMS from primary progressive MS (PPMS), while Cu significantly differentiated SPMS from PPMS. Additionally, Cu showed a negative correlation with MSSS, while Mn and Zn showed a positive correlation with EDSS. Conclusion  These findings underscore a substantive deficiency in Mn, Co, Zn, and Se in the MS cohort, supporting targeted supplementation with these trace elements. This study provides a comprehensive understanding of the intricate relationship between essential trace elements and MS, paving the way for further research into personalized nutritional interventions for this complex neurological disorder.",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Essential trace element levels in multiple sclerosis: bridging demographic and clinical gaps, assessing the need for supplementation",
volume = "83",
pages = "127421",
doi = "10.1016/j.jtemb.2024.127421"
}

Stojsavljević, A., Jagodić, J., Pavlović, S., Dinčić, E., Kuveljić, J., Manojlović, D.,& Živković, M.. (2024). Essential trace element levels in multiple sclerosis: bridging demographic and clinical gaps, assessing the need for supplementation. in Journal of Trace Elements in Medicine and Biology, 83, 127421.
https://doi.org/10.1016/j.jtemb.2024.127421

Stojsavljević A, Jagodić J, Pavlović S, Dinčić E, Kuveljić J, Manojlović D, Živković M. Essential trace element levels in multiple sclerosis: bridging demographic and clinical gaps, assessing the need for supplementation. in Journal of Trace Elements in Medicine and Biology. 2024;83:127421.
doi:10.1016/j.jtemb.2024.127421 .

Stojsavljević, Aleksandar, Jagodić, Jovana, Pavlović, Slađan, Dinčić, Evica, Kuveljić, Jovana, Manojlović, Dragan, Živković, Maja, "Essential trace element levels in multiple sclerosis: bridging demographic and clinical gaps, assessing the need for supplementation" in Journal of Trace Elements in Medicine and Biology, 83 (2024):127421,
https://doi.org/10.1016/j.jtemb.2024.127421 . .

TY  - JOUR
AU  - Kolić, Ivana
AU  - Stojković, Ljiljana S.
AU  - Stanković, Aleksandra
AU  - Stefanović, Milan
AU  - Dinčić, Evica
AU  - Živković, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9542
AB  - Background: Leptin (LEP), leptin receptor (LEPR) and peroxisome proliferator-activated receptor gamma co-activator 1-alpha (PGC1A) are involved in the pathogenesis of multiple sclerosis (MS) by affecting the inflammatory response and reactive oxygen species production. LEP rs7799039 and LEPR rs1137101 genetic variants modify the serum LEP levels and PGC1A rs8192678 alters the PGC1A activity. The study objective was to explore the associations of these variants with susceptibility to MS, disease course/clinical parameters and also with peripheral blood mononuclear cell expression of the target genes and plasma LEP concentrations, in the study subjects. Methods: The study groups included 528 patients with MS and 429 controls. TaqMan® assays were used for genotyping and gene expression quantification. The Chi-square, parametric and nonparametric tests and simple/multiple logistic regression were performed for the statistical analysis of data. Results: A multiple logistic regression model including all three investigated variants, applied to patients (RRMS + SPMS) and controls, showed that PGC1A rs8192678 minor allele had an increased risk for the occurrence of disease, with OR (95%CI) = 1,32 (1,01–1,73), P = 0,04. Between-effect of gender and LEPR variant on the multiple sclerosis severity score (MSSS) was identified (P = 0,005). In male patients (relapsing-remitting and secondary progressive), LEPR minor allele carriers had increased MSSS (GG + AG vs AA, median (minimum–maximum) = 5,38 (0,64–9,88) vs 4,27 (0,78–9,63); P = 0,01, Padj = 0,03). In relapsing-remitting patients LEP rs7799039 affected the LEP gene expression (P = 0,006; Padj = 0,04). Conclusion: The current findings implicate an impact of investigated genetic variants on the pathogenesis of MS. © 2021 Elsevier B.V.
T2  - Gene
T1  - Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis
VL  - 774
SP  - 145422
DO  - 10.1016/j.gene.2021.145422
ER  - 

@article{
author = "Kolić, Ivana and Stojković, Ljiljana S. and Stanković, Aleksandra and Stefanović, Milan and Dinčić, Evica and Živković, Maja",
year = "2021",
abstract = "Background: Leptin (LEP), leptin receptor (LEPR) and peroxisome proliferator-activated receptor gamma co-activator 1-alpha (PGC1A) are involved in the pathogenesis of multiple sclerosis (MS) by affecting the inflammatory response and reactive oxygen species production. LEP rs7799039 and LEPR rs1137101 genetic variants modify the serum LEP levels and PGC1A rs8192678 alters the PGC1A activity. The study objective was to explore the associations of these variants with susceptibility to MS, disease course/clinical parameters and also with peripheral blood mononuclear cell expression of the target genes and plasma LEP concentrations, in the study subjects. Methods: The study groups included 528 patients with MS and 429 controls. TaqMan® assays were used for genotyping and gene expression quantification. The Chi-square, parametric and nonparametric tests and simple/multiple logistic regression were performed for the statistical analysis of data. Results: A multiple logistic regression model including all three investigated variants, applied to patients (RRMS + SPMS) and controls, showed that PGC1A rs8192678 minor allele had an increased risk for the occurrence of disease, with OR (95%CI) = 1,32 (1,01–1,73), P = 0,04. Between-effect of gender and LEPR variant on the multiple sclerosis severity score (MSSS) was identified (P = 0,005). In male patients (relapsing-remitting and secondary progressive), LEPR minor allele carriers had increased MSSS (GG + AG vs AA, median (minimum–maximum) = 5,38 (0,64–9,88) vs 4,27 (0,78–9,63); P = 0,01, Padj = 0,03). In relapsing-remitting patients LEP rs7799039 affected the LEP gene expression (P = 0,006; Padj = 0,04). Conclusion: The current findings implicate an impact of investigated genetic variants on the pathogenesis of MS. © 2021 Elsevier B.V.",
journal = "Gene",
title = "Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis",
volume = "774",
pages = "145422",
doi = "10.1016/j.gene.2021.145422"
}

Kolić, I., Stojković, L. S., Stanković, A., Stefanović, M., Dinčić, E.,& Živković, M.. (2021). Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis. in Gene, 774, 145422.
https://doi.org/10.1016/j.gene.2021.145422

Kolić I, Stojković LS, Stanković A, Stefanović M, Dinčić E, Živković M. Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis. in Gene. 2021;774:145422.
doi:10.1016/j.gene.2021.145422 .

Kolić, Ivana, Stojković, Ljiljana S., Stanković, Aleksandra, Stefanović, Milan, Dinčić, Evica, Živković, Maja, "Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis" in Gene, 774 (2021):145422,
https://doi.org/10.1016/j.gene.2021.145422 . .

TY  - JOUR
AU  - Kolić, Ivana
AU  - Stojković, Ljiljana S.
AU  - Dinčić, Evica
AU  - Jovanović, Ivan G.
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8618
AB  - Leptin (LEP) may contribute to the pathogenesis of multiple sclerosis (MS) by its immunomodulatory, proinflammatory and prooxidant effects. Therefore, plasma LEP levels and mRNA expression of five genes related to the LEP signaling pathway (LEP, LEP receptor (LEPR), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1A), superoxide dismutase 2, tumor necrosis factor-alpha) were investigated in relapsing-remitting MS. In patients (N = 64), compared to healthy subjects (N = 62), relative LEP mRNA levels were significantly increased (p = 0,01), while LEPR and PGC1A mRNA levels were decreased (p = 0,001 and p = 0,04, respectively). Significant positive correlation was observed between LEPR mRNA levels and clinical parameters of MS progression (EDSS, MSSS). © 2019
T2  - Journal of Neuroimmunology
T1  - Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis
VL  - 338
SP  - 577090
DO  - 10.1016/j.jneuroim.2019.577090
ER  - 

@article{
author = "Kolić, Ivana and Stojković, Ljiljana S. and Dinčić, Evica and Jovanović, Ivan G. and Stanković, Aleksandra and Živković, Maja",
year = "2020",
abstract = "Leptin (LEP) may contribute to the pathogenesis of multiple sclerosis (MS) by its immunomodulatory, proinflammatory and prooxidant effects. Therefore, plasma LEP levels and mRNA expression of five genes related to the LEP signaling pathway (LEP, LEP receptor (LEPR), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1A), superoxide dismutase 2, tumor necrosis factor-alpha) were investigated in relapsing-remitting MS. In patients (N = 64), compared to healthy subjects (N = 62), relative LEP mRNA levels were significantly increased (p = 0,01), while LEPR and PGC1A mRNA levels were decreased (p = 0,001 and p = 0,04, respectively). Significant positive correlation was observed between LEPR mRNA levels and clinical parameters of MS progression (EDSS, MSSS). © 2019",
journal = "Journal of Neuroimmunology",
title = "Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis",
volume = "338",
pages = "577090",
doi = "10.1016/j.jneuroim.2019.577090"
}

Kolić, I., Stojković, L. S., Dinčić, E., Jovanović, I. G., Stanković, A.,& Živković, M.. (2020). Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis. in Journal of Neuroimmunology, 338, 577090.
https://doi.org/10.1016/j.jneuroim.2019.577090

Kolić I, Stojković LS, Dinčić E, Jovanović IG, Stanković A, Živković M. Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis. in Journal of Neuroimmunology. 2020;338:577090.
doi:10.1016/j.jneuroim.2019.577090 .

Kolić, Ivana, Stojković, Ljiljana S., Dinčić, Evica, Jovanović, Ivan G., Stanković, Aleksandra, Živković, Maja, "Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis" in Journal of Neuroimmunology, 338 (2020):577090,
https://doi.org/10.1016/j.jneuroim.2019.577090 . .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
AU  - Dinčić, Evica
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9725
AB  - Background/Aim. In vitro and in vivo studies show that CX3CL1 and CXCL16 chemokines and their specific receptors, CX3CR1 and CXCR6, respectively, mediate mechanism of neuroinflammation during the pathogenesis of multiple sclerosis (MS). The aim of this study was to investigate relative messenger ribonucleic acid (mRNA) levels of CX3CL1, CXCL16, CX3CR1 and CXCR6 in peripheral blood mononuclear cells, as potential molecular markers of relapsing-remitting (RR) MS. Methods. The study included 43 unrelated RR MS patients, 20 of them with clinically active disease (relapse) and 23 with clinically stable disease (remission), and 28 unrelated healthy subjects as controls. Real-time polymerase chain reactions (PCR) were performed using TaqMan® gene expression assays. Relative expression (mRNA) level of each target gene in each sample of peripheral blood mononuclear cells was calculated as the mean normalized expression. Results. The levels of CX3CR1 mRNA were significantly higher in clinically active RR MS patients compared to controls [fold change = 1.38, p (Mann-Whitney U test) = 0.009], and significantly lower in clinically stable vs active RR MS patients [fold change = - 1.43, p (t-test) = 0.03]. Stable RR MS patients had significantly higher CXCL16 mRNA levels than controls [fold change = 1.33, p (Mann-Whitney U test) = 0.006]. A trend of increased CXCR6 gene expression was found in active RR MS patients compared to controls [fold change = 1.23, p (Mann-Whitney U test) = 0.08]. In either active or stable RR MS patients there were no significant correlations of the clinical parameters with expression levels of the target genes. Conclusion. The current results show that increased CX3CR1 mRNA levels in peripheral blood mononuclear cells could represent a proinflammatory molecular marker of clinically active RR MS.
T2  - Vojnosanitetski pregled
T1  - Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study
VL  - 77
IS  - 9
SP  - 967
EP  - 973
DO  - 10.2298/VSP180717035S
ER  - 

@article{
author = "Stojković, Ljiljana S. and Stanković, Aleksandra and Životić, Ivan and Dinčić, Evica and Alavantić, Dragan and Živković, Maja",
year = "2020",
abstract = "Background/Aim. In vitro and in vivo studies show that CX3CL1 and CXCL16 chemokines and their specific receptors, CX3CR1 and CXCR6, respectively, mediate mechanism of neuroinflammation during the pathogenesis of multiple sclerosis (MS). The aim of this study was to investigate relative messenger ribonucleic acid (mRNA) levels of CX3CL1, CXCL16, CX3CR1 and CXCR6 in peripheral blood mononuclear cells, as potential molecular markers of relapsing-remitting (RR) MS. Methods. The study included 43 unrelated RR MS patients, 20 of them with clinically active disease (relapse) and 23 with clinically stable disease (remission), and 28 unrelated healthy subjects as controls. Real-time polymerase chain reactions (PCR) were performed using TaqMan® gene expression assays. Relative expression (mRNA) level of each target gene in each sample of peripheral blood mononuclear cells was calculated as the mean normalized expression. Results. The levels of CX3CR1 mRNA were significantly higher in clinically active RR MS patients compared to controls [fold change = 1.38, p (Mann-Whitney U test) = 0.009], and significantly lower in clinically stable vs active RR MS patients [fold change = - 1.43, p (t-test) = 0.03]. Stable RR MS patients had significantly higher CXCL16 mRNA levels than controls [fold change = 1.33, p (Mann-Whitney U test) = 0.006]. A trend of increased CXCR6 gene expression was found in active RR MS patients compared to controls [fold change = 1.23, p (Mann-Whitney U test) = 0.08]. In either active or stable RR MS patients there were no significant correlations of the clinical parameters with expression levels of the target genes. Conclusion. The current results show that increased CX3CR1 mRNA levels in peripheral blood mononuclear cells could represent a proinflammatory molecular marker of clinically active RR MS.",
journal = "Vojnosanitetski pregled",
title = "Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study",
volume = "77",
number = "9",
pages = "967-973",
doi = "10.2298/VSP180717035S"
}

Stojković, L. S., Stanković, A., Životić, I., Dinčić, E., Alavantić, D.,& Živković, M.. (2020). Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study. in Vojnosanitetski pregled, 77(9), 967-973.
https://doi.org/10.2298/VSP180717035S

Stojković LS, Stanković A, Životić I, Dinčić E, Alavantić D, Živković M. Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study. in Vojnosanitetski pregled. 2020;77(9):967-973.
doi:10.2298/VSP180717035S .

Stojković, Ljiljana S., Stanković, Aleksandra, Životić, Ivan, Dinčić, Evica, Alavantić, Dragan, Živković, Maja, "Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study" in Vojnosanitetski pregled, 77, no. 9 (2020):967-973,
https://doi.org/10.2298/VSP180717035S . .

TY  - JOUR
AU  - Stefanović, Milan
AU  - Životić, Ivan
AU  - Stojković, Ljiljana S.
AU  - Dinčić, Evica
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8885
AB  - An algorithm Probabilistic Identification of Causal SNPs, identified 434 causal variants for multiple sclerosis (MS) including IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510. Analysis of individual and combined effects of these variants in the Serbian population identified that Il2RA rs2104286 G allele carriers had a lower risk for developing MS (gender adjusted OR = 0.63, p = .003). With regard to the IFI30 rs11554159 recessive genetic model, among HLA-DRB1*15:01 positive patients, the AA homozygote had a significantly higher MSSS compared to the G allele carriers (p = .003). This study confirms role of IL2RA rs2104286 in MS and suggest the role of IFI30 rs11554159 in disease severity, which needs validation.
T2  - Journal of Neuroimmunology
T1  - The association of genetic variants IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510 with multiple sclerosis onset and severity in patients from Serbia
VL  - 347
SP  - 577346
DO  - 10.1016/j.jneuroim.2020.577346
ER  - 

@article{
author = "Stefanović, Milan and Životić, Ivan and Stojković, Ljiljana S. and Dinčić, Evica and Stanković, Aleksandra and Živković, Maja",
year = "2020",
abstract = "An algorithm Probabilistic Identification of Causal SNPs, identified 434 causal variants for multiple sclerosis (MS) including IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510. Analysis of individual and combined effects of these variants in the Serbian population identified that Il2RA rs2104286 G allele carriers had a lower risk for developing MS (gender adjusted OR = 0.63, p = .003). With regard to the IFI30 rs11554159 recessive genetic model, among HLA-DRB1*15:01 positive patients, the AA homozygote had a significantly higher MSSS compared to the G allele carriers (p = .003). This study confirms role of IL2RA rs2104286 in MS and suggest the role of IFI30 rs11554159 in disease severity, which needs validation.",
journal = "Journal of Neuroimmunology",
title = "The association of genetic variants IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510 with multiple sclerosis onset and severity in patients from Serbia",
volume = "347",
pages = "577346",
doi = "10.1016/j.jneuroim.2020.577346"
}

Stefanović, M., Životić, I., Stojković, L. S., Dinčić, E., Stanković, A.,& Živković, M.. (2020). The association of genetic variants IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510 with multiple sclerosis onset and severity in patients from Serbia. in Journal of Neuroimmunology, 347, 577346.
https://doi.org/10.1016/j.jneuroim.2020.577346

Stefanović M, Životić I, Stojković LS, Dinčić E, Stanković A, Živković M. The association of genetic variants IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510 with multiple sclerosis onset and severity in patients from Serbia. in Journal of Neuroimmunology. 2020;347:577346.
doi:10.1016/j.jneuroim.2020.577346 .

Stefanović, Milan, Životić, Ivan, Stojković, Ljiljana S., Dinčić, Evica, Stanković, Aleksandra, Živković, Maja, "The association of genetic variants IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510 with multiple sclerosis onset and severity in patients from Serbia" in Journal of Neuroimmunology, 347 (2020):577346,
https://doi.org/10.1016/j.jneuroim.2020.577346 . .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
AU  - Dinčić, Evica
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8884
AB  - Background/Aim. In vitro and in vivo studies show that CX3CL1 and CXCL16 chemokines and their specific receptors, CX3CR1 and CXCR6, respectively, mediate mechanism of neuroinflammation during the pathogenesis of multiple sclerosis (MS). The aim of this study was to investigate relative messenger ribonucleic acid (mRNA) levels of CX3CL1, CXCL16, CX3CR1 and CXCR6 in peripheral blood mononuclear cells, as potential molecular markers of relapsing-remitting (RR) MS. Methods. The study included 43 unrelated RR MS patients, 20 of them with clinically active disease (relapse) and 23 with clinically stable disease (remission), and 28 unrelated healthy subjects as controls. Real-time polymerase chain reactions (PCR) were performed using TaqMan? gene expression assays. Relative expression (mRNA) level of each target gene in each sample of peripheral blood mononuclear cells was calculated as the mean normalized expression. Results. The levels of CX3CR1 mRNA were significantly higher in clinically active RR MS patients compared to controls [fold change = 1.38, p (Mann-Whitney U test) = 0.009], and significantly lower in clinically stable vs active RR MS patients [fold change = - 1.43, p (t-test) = 0.03]. Stable RR MS patients had significantly higher CXCL16 mRNA levels than controls [fold change = 1.33, p (Mann-Whitney U test) = 0.006]. A trend of increased CXCR6 gene expression was found in active RR MS patients compared to controls [fold change = 1.23, p (Mann-Whitney U test) = 0.08]. In either active or stable RR MS patients there were no significant correlations of the clinical parameters with expression levels of the target genes. Conclusion. The current results show that increased CX3CR1 mRNA levels in peripheral blood mononuclear cells could represent a proinflammatory molecular marker of clinically active RR MS.
T2  - Vojnosanitetski pregled
T1  - Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study
VL  - 77
IS  - 9
SP  - 967
EP  - 973
DO  - 10.2298/VSP180717035S
ER  - 

@article{
author = "Stojković, Ljiljana S. and Stanković, Aleksandra and Životić, Ivan and Dinčić, Evica and Alavantić, Dragan and Živković, Maja",
year = "2020",
abstract = "Background/Aim. In vitro and in vivo studies show that CX3CL1 and CXCL16 chemokines and their specific receptors, CX3CR1 and CXCR6, respectively, mediate mechanism of neuroinflammation during the pathogenesis of multiple sclerosis (MS). The aim of this study was to investigate relative messenger ribonucleic acid (mRNA) levels of CX3CL1, CXCL16, CX3CR1 and CXCR6 in peripheral blood mononuclear cells, as potential molecular markers of relapsing-remitting (RR) MS. Methods. The study included 43 unrelated RR MS patients, 20 of them with clinically active disease (relapse) and 23 with clinically stable disease (remission), and 28 unrelated healthy subjects as controls. Real-time polymerase chain reactions (PCR) were performed using TaqMan? gene expression assays. Relative expression (mRNA) level of each target gene in each sample of peripheral blood mononuclear cells was calculated as the mean normalized expression. Results. The levels of CX3CR1 mRNA were significantly higher in clinically active RR MS patients compared to controls [fold change = 1.38, p (Mann-Whitney U test) = 0.009], and significantly lower in clinically stable vs active RR MS patients [fold change = - 1.43, p (t-test) = 0.03]. Stable RR MS patients had significantly higher CXCL16 mRNA levels than controls [fold change = 1.33, p (Mann-Whitney U test) = 0.006]. A trend of increased CXCR6 gene expression was found in active RR MS patients compared to controls [fold change = 1.23, p (Mann-Whitney U test) = 0.08]. In either active or stable RR MS patients there were no significant correlations of the clinical parameters with expression levels of the target genes. Conclusion. The current results show that increased CX3CR1 mRNA levels in peripheral blood mononuclear cells could represent a proinflammatory molecular marker of clinically active RR MS.",
journal = "Vojnosanitetski pregled",
title = "Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study",
volume = "77",
number = "9",
pages = "967-973",
doi = "10.2298/VSP180717035S"
}

Stojković, L. S., Stanković, A., Životić, I., Dinčić, E., Alavantić, D.,& Živković, M.. (2020). Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study. in Vojnosanitetski pregled, 77(9), 967-973.
https://doi.org/10.2298/VSP180717035S

Stojković LS, Stanković A, Životić I, Dinčić E, Alavantić D, Živković M. Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study. in Vojnosanitetski pregled. 2020;77(9):967-973.
doi:10.2298/VSP180717035S .

Stojković, Ljiljana S., Stanković, Aleksandra, Životić, Ivan, Dinčić, Evica, Alavantić, Dragan, Živković, Maja, "Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study" in Vojnosanitetski pregled, 77, no. 9 (2020):967-973,
https://doi.org/10.2298/VSP180717035S . .

TY  - JOUR
AU  - Lavtar, Polona
AU  - Rudolf, Gorazd
AU  - Maver, Aleš
AU  - Hodžić, Alenka
AU  - Starčević Čizmarević, Nada
AU  - Živković, Maja
AU  - Jazbec, Sasa Sega
AU  - Klemenc-Ketiš, Zalika
AU  - Kapović, Miljenko
AU  - Dinčić, Evica
AU  - Raičević, Ranko
AU  - Sepčić, Juraj
AU  - Lovrečić, Luca
AU  - Stanković, Aleksandra
AU  - Ristić, Smiljana
AU  - Peterlin, Borut
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1913
AB  - Prevalence of multiple sclerosis varies with geographic latitude. We hypothesized that this fact might be partially associated with the influence of latitude on circadian rhythm and consequently that genetic variability of key circadian rhythm regulators, ARNTL and CLOCK genes, might contribute to the risk for multiple sclerosis. Our aim was to analyse selected polymorphisms of ARNTL and CLOCK, and their association with multiple sclerosis. A total of 900 Caucasian patients and 1024 healthy controls were compared for genetic signature at 8 SNPs, 4 for each of both genes. We found a statistically significant difference in genotype (ARNTL rs3789327, P = 7.5.10(-5); CLOCK rs6811520 P = 0.02) distributions in patients and controls. The ARNTL rs3789327 CC genotype was associated with higher risk for multiple sclerosis at an OR of 1.67 (95% CI 1.35-2.07, P = 0.0001) and the CLOCK rs6811520 genotype CC at an OR of 1.40 (95% CI 1.13-1.73, P = 0.002). The results of this study suggest that genetic variability in the ARNTL and CLOCK genes might be associated with risk for multiple sclerosis.
T2  - PLOS One
T1  - Association of circadian rhythm genes ARNTL/BMAL1 and CLOCK with multiple sclerosis
VL  - 13
IS  - 1
SP  - e0190601
DO  - 10.1371/journal.pone.0190601
ER  - 

@article{
author = "Lavtar, Polona and Rudolf, Gorazd and Maver, Aleš and Hodžić, Alenka and Starčević Čizmarević, Nada and Živković, Maja and Jazbec, Sasa Sega and Klemenc-Ketiš, Zalika and Kapović, Miljenko and Dinčić, Evica and Raičević, Ranko and Sepčić, Juraj and Lovrečić, Luca and Stanković, Aleksandra and Ristić, Smiljana and Peterlin, Borut",
year = "2018",
abstract = "Prevalence of multiple sclerosis varies with geographic latitude. We hypothesized that this fact might be partially associated with the influence of latitude on circadian rhythm and consequently that genetic variability of key circadian rhythm regulators, ARNTL and CLOCK genes, might contribute to the risk for multiple sclerosis. Our aim was to analyse selected polymorphisms of ARNTL and CLOCK, and their association with multiple sclerosis. A total of 900 Caucasian patients and 1024 healthy controls were compared for genetic signature at 8 SNPs, 4 for each of both genes. We found a statistically significant difference in genotype (ARNTL rs3789327, P = 7.5.10(-5); CLOCK rs6811520 P = 0.02) distributions in patients and controls. The ARNTL rs3789327 CC genotype was associated with higher risk for multiple sclerosis at an OR of 1.67 (95% CI 1.35-2.07, P = 0.0001) and the CLOCK rs6811520 genotype CC at an OR of 1.40 (95% CI 1.13-1.73, P = 0.002). The results of this study suggest that genetic variability in the ARNTL and CLOCK genes might be associated with risk for multiple sclerosis.",
journal = "PLOS One",
title = "Association of circadian rhythm genes ARNTL/BMAL1 and CLOCK with multiple sclerosis",
volume = "13",
number = "1",
pages = "e0190601",
doi = "10.1371/journal.pone.0190601"
}

Lavtar, P., Rudolf, G., Maver, A., Hodžić, A., Starčević Čizmarević, N., Živković, M., Jazbec, S. S., Klemenc-Ketiš, Z., Kapović, M., Dinčić, E., Raičević, R., Sepčić, J., Lovrečić, L., Stanković, A., Ristić, S.,& Peterlin, B.. (2018). Association of circadian rhythm genes ARNTL/BMAL1 and CLOCK with multiple sclerosis. in PLOS One, 13(1), e0190601.
https://doi.org/10.1371/journal.pone.0190601

Lavtar P, Rudolf G, Maver A, Hodžić A, Starčević Čizmarević N, Živković M, Jazbec SS, Klemenc-Ketiš Z, Kapović M, Dinčić E, Raičević R, Sepčić J, Lovrečić L, Stanković A, Ristić S, Peterlin B. Association of circadian rhythm genes ARNTL/BMAL1 and CLOCK with multiple sclerosis. in PLOS One. 2018;13(1):e0190601.
doi:10.1371/journal.pone.0190601 .

Lavtar, Polona, Rudolf, Gorazd, Maver, Aleš, Hodžić, Alenka, Starčević Čizmarević, Nada, Živković, Maja, Jazbec, Sasa Sega, Klemenc-Ketiš, Zalika, Kapović, Miljenko, Dinčić, Evica, Raičević, Ranko, Sepčić, Juraj, Lovrečić, Luca, Stanković, Aleksandra, Ristić, Smiljana, Peterlin, Borut, "Association of circadian rhythm genes ARNTL/BMAL1 and CLOCK with multiple sclerosis" in PLOS One, 13, no. 1 (2018):e0190601,
https://doi.org/10.1371/journal.pone.0190601 . .

TY  - JOUR
AU  - Kostic, Smiljana
AU  - Kolić, Ivana
AU  - Raičević, Ranko
AU  - Stojanovic, Zvezdana
AU  - Kostić, Dejan
AU  - Dinčić, Evica
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1626
AB  - Background/Aim. Due to the fact that there is a relatively small number of data related to systemic insulin abnormalities in the multiple sclerosis (MS), the main objective of our study was to determine whether a dysbalance of glucose and insulin metabolism exist in patients with natural course of MS. Our hypothesis was that the metabolic disorder that characterizes state of the insulin resistance (IR) and reduced insulin sensitivity (IS) in untreated patients with MS could play a role in disease progression and degree of functional disability. Methods. The study included 31 patients with relapsing-remitting (RR) MS and 14 healthy controls from the same geographic area matched by age, ethnicity and number of smokers. The glucose tolerance, IS, and IR were examined using an oral glucose tolerance test (OGTT) and using basal plasma glucose and insulin levels. The functional disability and disease progression were evaluated by the Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS). Results. The MS patients tolerated glucose equally well as the healthy controls. Basal concentrations of insulin were significantly higher in the MS group p LT 0.05), as well as insulin plasma level 30 min after oral glucose load (p LT 0.01). The patients with MS had significantly higher values of homeostasis model assessment indexes of IR (HOMA-IR) (p = 0.027; p = 0.028). The percentage of IS (HOMA2 % S) and whole body IS index (ISI Matsuda) showed significantly lower values in the MS patients than in the controls (p = 0.005; p = 0.001). The insulinogenic index in the first 30 min of OGTT was significantly higher in MS patients (p = 0.005). The measures of functional disability and MS progression did not correlate significantly with the investigated parameters of IR and IS indexes. Conclusion. This study demonstrates for the first time the existence of hyperinsulinemia, reduced insulin sensitivity and normal glucose tolerance that indicate the initial phase of IR in the natural course of MS. Additional research is necessary in order to define the mechanisms of occurrence and the impact of IR on the complex pathophysiological processes in MS.
T2  - Vojnosanitetski pregled
T1  - Insulin resistance in drug naive patients with multiple sclerosis
VL  - 74
IS  - 6
SP  - 563
EP  - 570
DO  - 10.2298/VSP160218082K
ER  - 

@article{
author = "Kostic, Smiljana and Kolić, Ivana and Raičević, Ranko and Stojanovic, Zvezdana and Kostić, Dejan and Dinčić, Evica",
year = "2017",
abstract = "Background/Aim. Due to the fact that there is a relatively small number of data related to systemic insulin abnormalities in the multiple sclerosis (MS), the main objective of our study was to determine whether a dysbalance of glucose and insulin metabolism exist in patients with natural course of MS. Our hypothesis was that the metabolic disorder that characterizes state of the insulin resistance (IR) and reduced insulin sensitivity (IS) in untreated patients with MS could play a role in disease progression and degree of functional disability. Methods. The study included 31 patients with relapsing-remitting (RR) MS and 14 healthy controls from the same geographic area matched by age, ethnicity and number of smokers. The glucose tolerance, IS, and IR were examined using an oral glucose tolerance test (OGTT) and using basal plasma glucose and insulin levels. The functional disability and disease progression were evaluated by the Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS). Results. The MS patients tolerated glucose equally well as the healthy controls. Basal concentrations of insulin were significantly higher in the MS group p LT 0.05), as well as insulin plasma level 30 min after oral glucose load (p LT 0.01). The patients with MS had significantly higher values of homeostasis model assessment indexes of IR (HOMA-IR) (p = 0.027; p = 0.028). The percentage of IS (HOMA2 % S) and whole body IS index (ISI Matsuda) showed significantly lower values in the MS patients than in the controls (p = 0.005; p = 0.001). The insulinogenic index in the first 30 min of OGTT was significantly higher in MS patients (p = 0.005). The measures of functional disability and MS progression did not correlate significantly with the investigated parameters of IR and IS indexes. Conclusion. This study demonstrates for the first time the existence of hyperinsulinemia, reduced insulin sensitivity and normal glucose tolerance that indicate the initial phase of IR in the natural course of MS. Additional research is necessary in order to define the mechanisms of occurrence and the impact of IR on the complex pathophysiological processes in MS.",
journal = "Vojnosanitetski pregled",
title = "Insulin resistance in drug naive patients with multiple sclerosis",
volume = "74",
number = "6",
pages = "563-570",
doi = "10.2298/VSP160218082K"
}

Kostic, S., Kolić, I., Raičević, R., Stojanovic, Z., Kostić, D.,& Dinčić, E.. (2017). Insulin resistance in drug naive patients with multiple sclerosis. in Vojnosanitetski pregled, 74(6), 563-570.
https://doi.org/10.2298/VSP160218082K

Kostic S, Kolić I, Raičević R, Stojanovic Z, Kostić D, Dinčić E. Insulin resistance in drug naive patients with multiple sclerosis. in Vojnosanitetski pregled. 2017;74(6):563-570.
doi:10.2298/VSP160218082K .

Kostic, Smiljana, Kolić, Ivana, Raičević, Ranko, Stojanovic, Zvezdana, Kostić, Dejan, Dinčić, Evica, "Insulin resistance in drug naive patients with multiple sclerosis" in Vojnosanitetski pregled, 74, no. 6 (2017):563-570,
https://doi.org/10.2298/VSP160218082K . .

TY  - CONF
AU  - Kostic, S.
AU  - Dinčić, Evica
AU  - Živković, Maja
AU  - Raicevic, R.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7184
C3  - Multiple Sclerosis
T1  - Insulin resistance and multiple sclerosis: a pilot study in drug naive patients
VL  - 23
SP  - 763
EP  - 763
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7184
ER  - 

@conference{
author = "Kostic, S. and Dinčić, Evica and Živković, Maja and Raicevic, R.",
year = "2017",
journal = "Multiple Sclerosis",
title = "Insulin resistance and multiple sclerosis: a pilot study in drug naive patients",
volume = "23",
pages = "763-763",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7184"
}

Kostic, S., Dinčić, E., Živković, M.,& Raicevic, R.. (2017). Insulin resistance and multiple sclerosis: a pilot study in drug naive patients. in Multiple Sclerosis, 23, 763-763.
https://hdl.handle.net/21.15107/rcub_vinar_7184

Kostic S, Dinčić E, Živković M, Raicevic R. Insulin resistance and multiple sclerosis: a pilot study in drug naive patients. in Multiple Sclerosis. 2017;23:763-763.
https://hdl.handle.net/21.15107/rcub_vinar_7184 .

Kostic, S., Dinčić, Evica, Živković, Maja, Raicevic, R., "Insulin resistance and multiple sclerosis: a pilot study in drug naive patients" in Multiple Sclerosis, 23 (2017):763-763,
https://hdl.handle.net/21.15107/rcub_vinar_7184 .

TY  - JOUR
AU  - Živković, Maja
AU  - Kolaković, Ana
AU  - Stojković, Ljiljana S.
AU  - Dinčić, Evica
AU  - Kostic, Smiljana
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1016
AB  - The components of renin-angiotensin system, such as angiotensin-converting enzyme (ACE), angiotensin II and angiotensin II receptor type 1 and 2 (AT1R and AT2R), are expressed in the central nervous system and leukocytes and proposed to be involved in the inflammation and pathogenesis of multiple sclerosis (MS). ACE I/D, AT1R 1166A/C and AT2R-1332A/G are functional polymorphisms associated with phenotypes of diverse chronic inflammatory diseases. The aim of this study was to investigate the association between ACE I/D, AT1R 1166A/C and AT2R-1332A/G gene polymorphisms and MS in Serbian population. A total of 470 MS patients and 478 controls participated in the study. Allele-specific polymerase chain reaction (PCR) was performed for genotyping of the ACE polymorphism. The AT1R and AT2R genotyping was done by duplex PCR and restriction fragment length polymorphism analysis. Both ACE homozygotes, II and DD, were significantly overrepresented in MS patients, compared to controls (chi(2) test p = 0.03). Neither genotype nor allele frequencies of AT1R 1166A/C polymorphism were significantly different between patients and controls. Significant overrepresentation of AT2R-1332 AA genotype in female patients, compared to female controls, was detected (OR = 1.67, 95%CI = 1.13-2.49, chi(2) test p = 0.01), suggesting that this genotype could be a gender-specific genetic risk factor for MS. (C) 2016 Elsevier B.V. All rights reserved.
T2  - Journal of the Neurological Sciences
T1  - Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis
VL  - 363
SP  - 29
EP  - 32
DO  - 10.1016/j.jns.2016.02.026
ER  - 

@article{
author = "Živković, Maja and Kolaković, Ana and Stojković, Ljiljana S. and Dinčić, Evica and Kostic, Smiljana and Alavantić, Dragan and Stanković, Aleksandra",
year = "2016",
abstract = "The components of renin-angiotensin system, such as angiotensin-converting enzyme (ACE), angiotensin II and angiotensin II receptor type 1 and 2 (AT1R and AT2R), are expressed in the central nervous system and leukocytes and proposed to be involved in the inflammation and pathogenesis of multiple sclerosis (MS). ACE I/D, AT1R 1166A/C and AT2R-1332A/G are functional polymorphisms associated with phenotypes of diverse chronic inflammatory diseases. The aim of this study was to investigate the association between ACE I/D, AT1R 1166A/C and AT2R-1332A/G gene polymorphisms and MS in Serbian population. A total of 470 MS patients and 478 controls participated in the study. Allele-specific polymerase chain reaction (PCR) was performed for genotyping of the ACE polymorphism. The AT1R and AT2R genotyping was done by duplex PCR and restriction fragment length polymorphism analysis. Both ACE homozygotes, II and DD, were significantly overrepresented in MS patients, compared to controls (chi(2) test p = 0.03). Neither genotype nor allele frequencies of AT1R 1166A/C polymorphism were significantly different between patients and controls. Significant overrepresentation of AT2R-1332 AA genotype in female patients, compared to female controls, was detected (OR = 1.67, 95%CI = 1.13-2.49, chi(2) test p = 0.01), suggesting that this genotype could be a gender-specific genetic risk factor for MS. (C) 2016 Elsevier B.V. All rights reserved.",
journal = "Journal of the Neurological Sciences",
title = "Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis",
volume = "363",
pages = "29-32",
doi = "10.1016/j.jns.2016.02.026"
}

Živković, M., Kolaković, A., Stojković, L. S., Dinčić, E., Kostic, S., Alavantić, D.,& Stanković, A.. (2016). Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis. in Journal of the Neurological Sciences, 363, 29-32.
https://doi.org/10.1016/j.jns.2016.02.026

Živković M, Kolaković A, Stojković LS, Dinčić E, Kostic S, Alavantić D, Stanković A. Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis. in Journal of the Neurological Sciences. 2016;363:29-32.
doi:10.1016/j.jns.2016.02.026 .

Živković, Maja, Kolaković, Ana, Stojković, Ljiljana S., Dinčić, Evica, Kostic, Smiljana, Alavantić, Dragan, Stanković, Aleksandra, "Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis" in Journal of the Neurological Sciences, 363 (2016):29-32,
https://doi.org/10.1016/j.jns.2016.02.026 . .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
AU  - Dinčić, Evica
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/61
AB  - CXC ligand 16 (CXCL16) is a multifunctional chemokine involved in cell adhesion and chemoattraction as well as in the scavenging of oxidized lipoproteins. Experimental data suggest the roles of CXCL16 in pathogenesis of multiple sclerosis (MS). A181V polymorphism in the human CXCL16 gene has been associated with the clinical course of certain chronic inflammatory diseases. The aim of this study was to analyze the effects of CXCL16 A181V polymorphism on: (1) susceptibility to MS and disease course, (2) peripheral blood mononuclear cells (PBMC) CXCL16 mRNA levels and plasma soluble CXCL16 levels of patients with MS and healthy controls. In this study, 459 MS patients and 303 controls were included. Real-time PCR-based methods were applied for genotyping of CXCL16 A181V and for CXCL16 gene expression analysis. Quantitative sandwich enzyme immunoassay was performed for quantification of plasma soluble CXCL16. CXCL16 AA genotype had a significant protective effect on MS susceptibility in women (OR = 0.53, +/- 95 % CI = 0.35-0.82, p = 0.004). The V allele-containing genotypes were associated with significantly higher CXCL16 mRNA levels in PBMC of both female (mean factor = 1.81, S.E. = 1.14-2.77, p LT 0.01) and male (mean factor = 1.58, S.E. = 1.35-1.73, p LT 0.01) controls. No significant association of the CXCL16 polymorphism was established either with soluble CXCL16 plasma levels or with clinical parameters and course of MS. The main finding of this study is gender-specific association of CXCL16 A181V polymorphism with susceptibility to MS in females. The current results should be replicated and validated in the larger sample group.
T2  - Journal of Neurology
T1  - The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings
VL  - 261
IS  - 8
SP  - 1544
EP  - 1551
DO  - 10.1007/s00415-014-7379-7
ER  - 

@article{
author = "Stojković, Ljiljana S. and Stanković, Aleksandra and Đurić, Tamara and Dinčić, Evica and Alavantić, Dragan and Živković, Maja",
year = "2014",
abstract = "CXC ligand 16 (CXCL16) is a multifunctional chemokine involved in cell adhesion and chemoattraction as well as in the scavenging of oxidized lipoproteins. Experimental data suggest the roles of CXCL16 in pathogenesis of multiple sclerosis (MS). A181V polymorphism in the human CXCL16 gene has been associated with the clinical course of certain chronic inflammatory diseases. The aim of this study was to analyze the effects of CXCL16 A181V polymorphism on: (1) susceptibility to MS and disease course, (2) peripheral blood mononuclear cells (PBMC) CXCL16 mRNA levels and plasma soluble CXCL16 levels of patients with MS and healthy controls. In this study, 459 MS patients and 303 controls were included. Real-time PCR-based methods were applied for genotyping of CXCL16 A181V and for CXCL16 gene expression analysis. Quantitative sandwich enzyme immunoassay was performed for quantification of plasma soluble CXCL16. CXCL16 AA genotype had a significant protective effect on MS susceptibility in women (OR = 0.53, +/- 95 % CI = 0.35-0.82, p = 0.004). The V allele-containing genotypes were associated with significantly higher CXCL16 mRNA levels in PBMC of both female (mean factor = 1.81, S.E. = 1.14-2.77, p LT 0.01) and male (mean factor = 1.58, S.E. = 1.35-1.73, p LT 0.01) controls. No significant association of the CXCL16 polymorphism was established either with soluble CXCL16 plasma levels or with clinical parameters and course of MS. The main finding of this study is gender-specific association of CXCL16 A181V polymorphism with susceptibility to MS in females. The current results should be replicated and validated in the larger sample group.",
journal = "Journal of Neurology",
title = "The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings",
volume = "261",
number = "8",
pages = "1544-1551",
doi = "10.1007/s00415-014-7379-7"
}

Stojković, L. S., Stanković, A., Đurić, T., Dinčić, E., Alavantić, D.,& Živković, M.. (2014). The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings. in Journal of Neurology, 261(8), 1544-1551.
https://doi.org/10.1007/s00415-014-7379-7

Stojković LS, Stanković A, Đurić T, Dinčić E, Alavantić D, Živković M. The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings. in Journal of Neurology. 2014;261(8):1544-1551.
doi:10.1007/s00415-014-7379-7 .

Stojković, Ljiljana S., Stanković, Aleksandra, Đurić, Tamara, Dinčić, Evica, Alavantić, Dragan, Živković, Maja, "The gender-specific association of CXCL16 A181V gene polymorphism with susceptibility to multiple sclerosis, and its effects on PBMC mRNA and plasma soluble CXCL16 levels: preliminary findings" in Journal of Neurology, 261, no. 8 (2014):1544-1551,
https://doi.org/10.1007/s00415-014-7379-7 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Starčević Čizmarević, Nada
AU  - Lovrečić, Luca
AU  - Klupka-Saric, Inge
AU  - Stanković, Aleksandra
AU  - Gasparovic, Iva
AU  - Lavtar, Polona
AU  - Dinčić, Evica
AU  - Stojković, Ljiljana S.
AU  - Rudolf, Gorazd
AU  - Jazbec, Sasa Sega
AU  - Perkovic, Olivio
AU  - Sinanovic, Osman
AU  - Sepčić, Juraj
AU  - Kapović, Miljenko
AU  - Peterlin, Borut
AU  - Ristić, Smiljana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5963
AB  - Background. Previous studies have shown impaired fibrinolysis in multiple sclerosis (MS) and implicated extracellular proteolytic enzymes as important factors in demyelinating neuroinflammatory disorders. Tissue-type plasminogen activator (t-PA) and its inhibitor (PAI-1) are key molecules in both fibrinolysis and extracellular proteolysis. In the present study, an association of the TPA Alu I/D and PAI-1 4G/5G polymorphisms with MS was analyzed within the Genomic Network for Multiple Sclerosis (GENoMS). Methods. The GENoMS includes four populations (Croatian, Slovenian, Serbian, and Bosnian and Herzegovinian) sharing the same geographic location and a similar ethnic background. A total of 885 patients and 656 ethnically matched healthy blood donors with no history of MS in their families were genotyped using PCR-RFLP. Results. TPA DD homozygosity was protective (OR = 0.79, 95% CI 0.63-0.99, P = 0.037) and PAI 5G5G was a risk factor for MS (OR = 1.30, 95% CI 1.01-1.66, P = 0.038). A significant effect of the genotype/carrier combination was detected in 5G5G/I carriers (OR = 1.39 95% CI 1.06-1.82, P = 0.017). Conclusions. We found a significantly harmful effect of the combination of the PAI-1 5G/5G genotype and TPA I allele on MS susceptibility, which indicates the importance of gene-gene interactions in complex diseases such as MS.
T2  - Disease Markers
T1  - The Role of TPA I/D and PAI-1 4G/5G Polymorphisms in Multiple Sclerosis
DO  - 10.1155/2014/362708
ER  - 

@article{
author = "Živković, Maja and Starčević Čizmarević, Nada and Lovrečić, Luca and Klupka-Saric, Inge and Stanković, Aleksandra and Gasparovic, Iva and Lavtar, Polona and Dinčić, Evica and Stojković, Ljiljana S. and Rudolf, Gorazd and Jazbec, Sasa Sega and Perkovic, Olivio and Sinanovic, Osman and Sepčić, Juraj and Kapović, Miljenko and Peterlin, Borut and Ristić, Smiljana",
year = "2014",
abstract = "Background. Previous studies have shown impaired fibrinolysis in multiple sclerosis (MS) and implicated extracellular proteolytic enzymes as important factors in demyelinating neuroinflammatory disorders. Tissue-type plasminogen activator (t-PA) and its inhibitor (PAI-1) are key molecules in both fibrinolysis and extracellular proteolysis. In the present study, an association of the TPA Alu I/D and PAI-1 4G/5G polymorphisms with MS was analyzed within the Genomic Network for Multiple Sclerosis (GENoMS). Methods. The GENoMS includes four populations (Croatian, Slovenian, Serbian, and Bosnian and Herzegovinian) sharing the same geographic location and a similar ethnic background. A total of 885 patients and 656 ethnically matched healthy blood donors with no history of MS in their families were genotyped using PCR-RFLP. Results. TPA DD homozygosity was protective (OR = 0.79, 95% CI 0.63-0.99, P = 0.037) and PAI 5G5G was a risk factor for MS (OR = 1.30, 95% CI 1.01-1.66, P = 0.038). A significant effect of the genotype/carrier combination was detected in 5G5G/I carriers (OR = 1.39 95% CI 1.06-1.82, P = 0.017). Conclusions. We found a significantly harmful effect of the combination of the PAI-1 5G/5G genotype and TPA I allele on MS susceptibility, which indicates the importance of gene-gene interactions in complex diseases such as MS.",
journal = "Disease Markers",
title = "The Role of TPA I/D and PAI-1 4G/5G Polymorphisms in Multiple Sclerosis",
doi = "10.1155/2014/362708"
}

Živković, M., Starčević Čizmarević, N., Lovrečić, L., Klupka-Saric, I., Stanković, A., Gasparovic, I., Lavtar, P., Dinčić, E., Stojković, L. S., Rudolf, G., Jazbec, S. S., Perkovic, O., Sinanovic, O., Sepčić, J., Kapović, M., Peterlin, B.,& Ristić, S.. (2014). The Role of TPA I/D and PAI-1 4G/5G Polymorphisms in Multiple Sclerosis. in Disease Markers.
https://doi.org/10.1155/2014/362708

Živković M, Starčević Čizmarević N, Lovrečić L, Klupka-Saric I, Stanković A, Gasparovic I, Lavtar P, Dinčić E, Stojković LS, Rudolf G, Jazbec SS, Perkovic O, Sinanovic O, Sepčić J, Kapović M, Peterlin B, Ristić S. The Role of TPA I/D and PAI-1 4G/5G Polymorphisms in Multiple Sclerosis. in Disease Markers. 2014;.
doi:10.1155/2014/362708 .

Živković, Maja, Starčević Čizmarević, Nada, Lovrečić, Luca, Klupka-Saric, Inge, Stanković, Aleksandra, Gasparovic, Iva, Lavtar, Polona, Dinčić, Evica, Stojković, Ljiljana S., Rudolf, Gorazd, Jazbec, Sasa Sega, Perkovic, Olivio, Sinanovic, Osman, Sepčić, Juraj, Kapović, Miljenko, Peterlin, Borut, Ristić, Smiljana, "The Role of TPA I/D and PAI-1 4G/5G Polymorphisms in Multiple Sclerosis" in Disease Markers (2014),
https://doi.org/10.1155/2014/362708 . .

TY  - JOUR
AU  - Lukić, Nikola
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Bundalo, Maja M.
AU  - Krsmanovic, Z.
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5506
AB  - The function of peroxisome proliferator-activated receptor gamma (PPAR gamma) in immune regulation, as well as in anti-inflammatory and anti-proliferative actions towards T lymphocytes, has been reported. A potential role of PPARs in multiple sclerosis (MS) was suggested. The aim of this study was to investigate if there is an association of PPAR gamma-2 Pro12Ala polymorphism with MS in 361 patients from Serbia. The genotype and allele frequencies of Pro12Ala polymorphism were not significantly different between controls and patients, or between females and males. In contrast to controls, we detected a rare Ala/Ala genotype in patients with MS. We found that there is a significant association of Ala/Ala genotype with older age at onset (ANOVA, p=0.07; LSD post-hoc, Ala/Ala vs. Pro/Ala, p=0.03, Ala/Ala vs. Pro/Pro p=0.02). It would be useful to validate our results in other populations, as well as to perform follow-up of the disease progression in regard to PPAR gamma genotypes.
T2  - Archives of Biological Sciences
T1  - The Ala/Ala Genotype of Pparγ Pro12 Ala Polymorphism Is Associated with Late Onset of Multiple Sclerosis
VL  - 65
IS  - 2
SP  - 447
EP  - 453
DO  - 10.2298/ABS1302447L
ER  - 

@article{
author = "Lukić, Nikola and Stanković, Aleksandra and Dinčić, Evica and Bundalo, Maja M. and Krsmanovic, Z. and Alavantić, Dragan and Živković, Maja",
year = "2013",
abstract = "The function of peroxisome proliferator-activated receptor gamma (PPAR gamma) in immune regulation, as well as in anti-inflammatory and anti-proliferative actions towards T lymphocytes, has been reported. A potential role of PPARs in multiple sclerosis (MS) was suggested. The aim of this study was to investigate if there is an association of PPAR gamma-2 Pro12Ala polymorphism with MS in 361 patients from Serbia. The genotype and allele frequencies of Pro12Ala polymorphism were not significantly different between controls and patients, or between females and males. In contrast to controls, we detected a rare Ala/Ala genotype in patients with MS. We found that there is a significant association of Ala/Ala genotype with older age at onset (ANOVA, p=0.07; LSD post-hoc, Ala/Ala vs. Pro/Ala, p=0.03, Ala/Ala vs. Pro/Pro p=0.02). It would be useful to validate our results in other populations, as well as to perform follow-up of the disease progression in regard to PPAR gamma genotypes.",
journal = "Archives of Biological Sciences",
title = "The Ala/Ala Genotype of Pparγ Pro12 Ala Polymorphism Is Associated with Late Onset of Multiple Sclerosis",
volume = "65",
number = "2",
pages = "447-453",
doi = "10.2298/ABS1302447L"
}

Lukić, N., Stanković, A., Dinčić, E., Bundalo, M. M., Krsmanovic, Z., Alavantić, D.,& Živković, M.. (2013). The Ala/Ala Genotype of Pparγ Pro12 Ala Polymorphism Is Associated with Late Onset of Multiple Sclerosis. in Archives of Biological Sciences, 65(2), 447-453.
https://doi.org/10.2298/ABS1302447L

Lukić N, Stanković A, Dinčić E, Bundalo MM, Krsmanovic Z, Alavantić D, Živković M. The Ala/Ala Genotype of Pparγ Pro12 Ala Polymorphism Is Associated with Late Onset of Multiple Sclerosis. in Archives of Biological Sciences. 2013;65(2):447-453.
doi:10.2298/ABS1302447L .

Lukić, Nikola, Stanković, Aleksandra, Dinčić, Evica, Bundalo, Maja M., Krsmanovic, Z., Alavantić, Dragan, Živković, Maja, "The Ala/Ala Genotype of Pparγ Pro12 Ala Polymorphism Is Associated with Late Onset of Multiple Sclerosis" in Archives of Biological Sciences, 65, no. 2 (2013):447-453,
https://doi.org/10.2298/ABS1302447L . .

TY  - JOUR
AU  - Živković, Maja
AU  - Životić, Ivan
AU  - Dinčić, Evica
AU  - Stojković, Ljiljana S.
AU  - Vojinovic, Slobodan
AU  - Stanković, Aleksandra
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5771
AB  - Background: Multiple sclerosis (MS) occurs as a result of interaction between genetic and environmental factors. Recent data support the view that oxidative damage is one of an early event in MS tissue injury. The safe elimination of reactive oxygen species and toxins via glutathione S-transferase (GST) pathways is required in order to protect cells against reactive oxygen-induced damage. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the susceptibility and clinical parameters of MS, in 455 consecutive patients and 366 controls. Methods: A multiplex polymerase chain reaction (PCR) was used to detect the deletions in GSTM1 and GSTT1 genes. Results: Patients with MS had significantly higher frequency of GSTT1 null genotype compared to controls (37.36% vs. 21.86%, respectively, p LT 0.0001, adjusted OR 2.13 (1.56-2.90)), as well as double deletions (15.38% vs. 10.38%, respectively, p LT 0.05). The carriers of GSTM1 deletion had significantly earlier onset of MS compared to the wild-type carriers (28.31 +/- 8.45 vs. 30.64 +/- 9.30 years, respectively, p = 0.03). Conclusion: This study suggests the potential pathogenic role of GSTT1 deletion on MS susceptibility. There are no similar data published so far, yet this study should be replicated in other populations. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Journal of the Neurological Sciences
T1  - The glutathione S-transferase T1 deletion is associated with susceptibility to multiple sclerosis
VL  - 334
IS  - 1-2
SP  - 6
EP  - 9
DO  - 10.1016/j.jns.2013.07.001
ER  - 

@article{
author = "Živković, Maja and Životić, Ivan and Dinčić, Evica and Stojković, Ljiljana S. and Vojinovic, Slobodan and Stanković, Aleksandra",
year = "2013",
abstract = "Background: Multiple sclerosis (MS) occurs as a result of interaction between genetic and environmental factors. Recent data support the view that oxidative damage is one of an early event in MS tissue injury. The safe elimination of reactive oxygen species and toxins via glutathione S-transferase (GST) pathways is required in order to protect cells against reactive oxygen-induced damage. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the susceptibility and clinical parameters of MS, in 455 consecutive patients and 366 controls. Methods: A multiplex polymerase chain reaction (PCR) was used to detect the deletions in GSTM1 and GSTT1 genes. Results: Patients with MS had significantly higher frequency of GSTT1 null genotype compared to controls (37.36% vs. 21.86%, respectively, p LT 0.0001, adjusted OR 2.13 (1.56-2.90)), as well as double deletions (15.38% vs. 10.38%, respectively, p LT 0.05). The carriers of GSTM1 deletion had significantly earlier onset of MS compared to the wild-type carriers (28.31 +/- 8.45 vs. 30.64 +/- 9.30 years, respectively, p = 0.03). Conclusion: This study suggests the potential pathogenic role of GSTT1 deletion on MS susceptibility. There are no similar data published so far, yet this study should be replicated in other populations. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Journal of the Neurological Sciences",
title = "The glutathione S-transferase T1 deletion is associated with susceptibility to multiple sclerosis",
volume = "334",
number = "1-2",
pages = "6-9",
doi = "10.1016/j.jns.2013.07.001"
}

Živković, M., Životić, I., Dinčić, E., Stojković, L. S., Vojinovic, S.,& Stanković, A.. (2013). The glutathione S-transferase T1 deletion is associated with susceptibility to multiple sclerosis. in Journal of the Neurological Sciences, 334(1-2), 6-9.
https://doi.org/10.1016/j.jns.2013.07.001

Živković M, Životić I, Dinčić E, Stojković LS, Vojinovic S, Stanković A. The glutathione S-transferase T1 deletion is associated with susceptibility to multiple sclerosis. in Journal of the Neurological Sciences. 2013;334(1-2):6-9.
doi:10.1016/j.jns.2013.07.001 .

Živković, Maja, Životić, Ivan, Dinčić, Evica, Stojković, Ljiljana S., Vojinovic, Slobodan, Stanković, Aleksandra, "The glutathione S-transferase T1 deletion is associated with susceptibility to multiple sclerosis" in Journal of the Neurological Sciences, 334, no. 1-2 (2013):6-9,
https://doi.org/10.1016/j.jns.2013.07.001 . .

TY  - JOUR
AU  - Krsmanovic, Zeljko
AU  - Živković, Maja
AU  - Lepic, Toplica
AU  - Stanković, Aleksandra
AU  - Raičević, Ranko
AU  - Dinčić, Evica
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5618
AB  - Background: Recent evidence has indicated an association between chronic cerebrospinal venous insufficiency (CCSVI) and multiple sclerosis. Small internal jugular veins (IJVs) (with a cross-sectional area of less than 0.4 cm(2)) have been previously described as difficult to catheterize, and their presence may potentially affect cerebrospinal venous drainage. In this blinded extracranial color-Doppler study we had two principal aims: first, to assess prevalence of CCSVI among Serbian MS patients compared to healthy controls; and second, to assess prevalence of small IJVs (with a CSA LT = 0.4 cm(2)) among MS patients and controls. Methods: The sixty seven unrelated patients with clinical isolated syndrome (CIS), relapsing-remitting (RR), secondary progressive (SP) and primary progressive (PP) multiple sclerosis and 21 healthy controls were examined by high-resolution color-Doppler. Results: The ultrasonographic criteria of CCSVI (according to Zamboni) were positive in 11.9% of the patients and in none of the control subjects. The CCSVI-positive patients had significantly longer disease durations and were significantly more disabled (measured by their Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS) scores), but after adjustment for gender and disease duration, CCSVI was not an independent risk factor for multiple sclerosis severity. The small IJVs were found in 28.4% of the patients and 28.6% of the controls. The patients with small IJVs were associated with decreased venous outflow from the brain and presented with longer disease durations and significantly higher EDSS and MSSS scores compared to patients without small IJVs. A multivariate logistic regression analysis adjusted for gender and disease duration showed that small IJV is an independent factor associated with multiple sclerosis severity (EDSS GT = 6) (adjusted OR = 8.9, 95% CI: 1.8-45.6, p = 0.007). Among patients with small IJVs the 36.84% were also CCSVI positive. Conclusions: Both, CCSVI and small IJVs seem to influence or follow MS severity, but only small IJVs turned out to be an independent factor in this study. Thus, small IJVs with restricted outflow, which might be aspects of CCSVI different from the criteria originally described by Zamboni, emerge as a cofactor in the multifactorial pathophysiology of multiple sclerosis.
T2  - BMC Neurology
T1  - Small internal jugular veins with restricted outflow are associated with severe multiple sclerosis: a sonographer-blinded, case-control ultrasound study
VL  - 13
DO  - 10.1186/1471-2377-13-90
ER  - 

@article{
author = "Krsmanovic, Zeljko and Živković, Maja and Lepic, Toplica and Stanković, Aleksandra and Raičević, Ranko and Dinčić, Evica",
year = "2013",
abstract = "Background: Recent evidence has indicated an association between chronic cerebrospinal venous insufficiency (CCSVI) and multiple sclerosis. Small internal jugular veins (IJVs) (with a cross-sectional area of less than 0.4 cm(2)) have been previously described as difficult to catheterize, and their presence may potentially affect cerebrospinal venous drainage. In this blinded extracranial color-Doppler study we had two principal aims: first, to assess prevalence of CCSVI among Serbian MS patients compared to healthy controls; and second, to assess prevalence of small IJVs (with a CSA LT = 0.4 cm(2)) among MS patients and controls. Methods: The sixty seven unrelated patients with clinical isolated syndrome (CIS), relapsing-remitting (RR), secondary progressive (SP) and primary progressive (PP) multiple sclerosis and 21 healthy controls were examined by high-resolution color-Doppler. Results: The ultrasonographic criteria of CCSVI (according to Zamboni) were positive in 11.9% of the patients and in none of the control subjects. The CCSVI-positive patients had significantly longer disease durations and were significantly more disabled (measured by their Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS) scores), but after adjustment for gender and disease duration, CCSVI was not an independent risk factor for multiple sclerosis severity. The small IJVs were found in 28.4% of the patients and 28.6% of the controls. The patients with small IJVs were associated with decreased venous outflow from the brain and presented with longer disease durations and significantly higher EDSS and MSSS scores compared to patients without small IJVs. A multivariate logistic regression analysis adjusted for gender and disease duration showed that small IJV is an independent factor associated with multiple sclerosis severity (EDSS GT = 6) (adjusted OR = 8.9, 95% CI: 1.8-45.6, p = 0.007). Among patients with small IJVs the 36.84% were also CCSVI positive. Conclusions: Both, CCSVI and small IJVs seem to influence or follow MS severity, but only small IJVs turned out to be an independent factor in this study. Thus, small IJVs with restricted outflow, which might be aspects of CCSVI different from the criteria originally described by Zamboni, emerge as a cofactor in the multifactorial pathophysiology of multiple sclerosis.",
journal = "BMC Neurology",
title = "Small internal jugular veins with restricted outflow are associated with severe multiple sclerosis: a sonographer-blinded, case-control ultrasound study",
volume = "13",
doi = "10.1186/1471-2377-13-90"
}

Krsmanovic, Z., Živković, M., Lepic, T., Stanković, A., Raičević, R.,& Dinčić, E.. (2013). Small internal jugular veins with restricted outflow are associated with severe multiple sclerosis: a sonographer-blinded, case-control ultrasound study. in BMC Neurology, 13.
https://doi.org/10.1186/1471-2377-13-90

Krsmanovic Z, Živković M, Lepic T, Stanković A, Raičević R, Dinčić E. Small internal jugular veins with restricted outflow are associated with severe multiple sclerosis: a sonographer-blinded, case-control ultrasound study. in BMC Neurology. 2013;13.
doi:10.1186/1471-2377-13-90 .

Krsmanovic, Zeljko, Živković, Maja, Lepic, Toplica, Stanković, Aleksandra, Raičević, Ranko, Dinčić, Evica, "Small internal jugular veins with restricted outflow are associated with severe multiple sclerosis: a sonographer-blinded, case-control ultrasound study" in BMC Neurology, 13 (2013),
https://doi.org/10.1186/1471-2377-13-90 . .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Stančić, Olja
AU  - Veljković, Nevena V.
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4841
AB  - We investigated the association of CX3CR1 genotypes/haplotypes with MS and performed the prediction analysis of protein sequence variants effects on CX3CL1/CX3CR1 interaction. We found no association of CX3CR1 with MS susceptibility. Frequency of I249T280 haplotype was significantly lower in SP compared to RR patients (RR GT 10 years, OR=0.30, 95%CI=0.11-0.79, p=0.01: OR=0.53, 95%CI=0.18-1.56, p=0.2, in sP LT 10 years vs. RR GT 10 years). Prediction analysis showed that I249 T280 protein variant would significantly affect CX3CL1/CX3CR1 interaction. Our results suggest that CX3CR1 I249T280 haplotype could have protective effect for switch to SP MS. Further research is warranted to validate and replicate currently observed results. (C) 2012 Elsevier B.V. All rights reserved.
T2  - Journal of Neuroimmunology
T1  - The association of V249I and T280M fractalkine receptor haplotypes with disease course of multiple sclerosis
VL  - 245
IS  - 1-2
SP  - 87
EP  - 92
DO  - 10.1016/j.jneuroim.2011.12.028
ER  - 

@article{
author = "Stojković, Ljiljana S. and Đurić, Tamara and Stanković, Aleksandra and Dinčić, Evica and Stančić, Olja and Veljković, Nevena V. and Alavantić, Dragan and Živković, Maja",
year = "2012",
abstract = "We investigated the association of CX3CR1 genotypes/haplotypes with MS and performed the prediction analysis of protein sequence variants effects on CX3CL1/CX3CR1 interaction. We found no association of CX3CR1 with MS susceptibility. Frequency of I249T280 haplotype was significantly lower in SP compared to RR patients (RR GT 10 years, OR=0.30, 95%CI=0.11-0.79, p=0.01: OR=0.53, 95%CI=0.18-1.56, p=0.2, in sP LT 10 years vs. RR GT 10 years). Prediction analysis showed that I249 T280 protein variant would significantly affect CX3CL1/CX3CR1 interaction. Our results suggest that CX3CR1 I249T280 haplotype could have protective effect for switch to SP MS. Further research is warranted to validate and replicate currently observed results. (C) 2012 Elsevier B.V. All rights reserved.",
journal = "Journal of Neuroimmunology",
title = "The association of V249I and T280M fractalkine receptor haplotypes with disease course of multiple sclerosis",
volume = "245",
number = "1-2",
pages = "87-92",
doi = "10.1016/j.jneuroim.2011.12.028"
}

Stojković, L. S., Đurić, T., Stanković, A., Dinčić, E., Stančić, O., Veljković, N. V., Alavantić, D.,& Živković, M.. (2012). The association of V249I and T280M fractalkine receptor haplotypes with disease course of multiple sclerosis. in Journal of Neuroimmunology, 245(1-2), 87-92.
https://doi.org/10.1016/j.jneuroim.2011.12.028

Stojković LS, Đurić T, Stanković A, Dinčić E, Stančić O, Veljković NV, Alavantić D, Živković M. The association of V249I and T280M fractalkine receptor haplotypes with disease course of multiple sclerosis. in Journal of Neuroimmunology. 2012;245(1-2):87-92.
doi:10.1016/j.jneuroim.2011.12.028 .

Stojković, Ljiljana S., Đurić, Tamara, Stanković, Aleksandra, Dinčić, Evica, Stančić, Olja, Veljković, Nevena V., Alavantić, Dragan, Živković, Maja, "The association of V249I and T280M fractalkine receptor haplotypes with disease course of multiple sclerosis" in Journal of Neuroimmunology, 245, no. 1-2 (2012):87-92,
https://doi.org/10.1016/j.jneuroim.2011.12.028 . .

TY  - CONF
AU  - Lavtar, P.
AU  - Rudolf, G.
AU  - Maver, A.
AU  - Lovrecic, L.
AU  - Ristic, S.
AU  - Starčević Čizmarević, Nada
AU  - Kapovic, M.
AU  - Sepcic, J.
AU  - Stanković, Aleksandra
AU  - Živković, Maja
AU  - Dinčić, Evica
AU  - Raicevic, R.
AU  - Jazbec, S. Sega
AU  - Ketis, Z. Klemenc
AU  - Potisk, K. Peterlin
AU  - Peterlin, B.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6979
C3  - European Journal of Neurology
T1  - Circadian rhythm genes and multiple sclerosis (MS)
VL  - 19
SP  - 346
EP  - 346
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6979
ER  - 

@conference{
author = "Lavtar, P. and Rudolf, G. and Maver, A. and Lovrecic, L. and Ristic, S. and Starčević Čizmarević, Nada and Kapovic, M. and Sepcic, J. and Stanković, Aleksandra and Živković, Maja and Dinčić, Evica and Raicevic, R. and Jazbec, S. Sega and Ketis, Z. Klemenc and Potisk, K. Peterlin and Peterlin, B.",
year = "2012",
journal = "European Journal of Neurology",
title = "Circadian rhythm genes and multiple sclerosis (MS)",
volume = "19",
pages = "346-346",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6979"
}

Lavtar, P., Rudolf, G., Maver, A., Lovrecic, L., Ristic, S., Starčević Čizmarević, N., Kapovic, M., Sepcic, J., Stanković, A., Živković, M., Dinčić, E., Raicevic, R., Jazbec, S. S., Ketis, Z. K., Potisk, K. P.,& Peterlin, B.. (2012). Circadian rhythm genes and multiple sclerosis (MS). in European Journal of Neurology, 19, 346-346.
https://hdl.handle.net/21.15107/rcub_vinar_6979

Lavtar P, Rudolf G, Maver A, Lovrecic L, Ristic S, Starčević Čizmarević N, Kapovic M, Sepcic J, Stanković A, Živković M, Dinčić E, Raicevic R, Jazbec SS, Ketis ZK, Potisk KP, Peterlin B. Circadian rhythm genes and multiple sclerosis (MS). in European Journal of Neurology. 2012;19:346-346.
https://hdl.handle.net/21.15107/rcub_vinar_6979 .

Lavtar, P., Rudolf, G., Maver, A., Lovrecic, L., Ristic, S., Starčević Čizmarević, Nada, Kapovic, M., Sepcic, J., Stanković, Aleksandra, Živković, Maja, Dinčić, Evica, Raicevic, R., Jazbec, S. Sega, Ketis, Z. Klemenc, Potisk, K. Peterlin, Peterlin, B., "Circadian rhythm genes and multiple sclerosis (MS)" in European Journal of Neurology, 19 (2012):346-346,
https://hdl.handle.net/21.15107/rcub_vinar_6979 .

TY  - CONF
AU  - Stanković, Aleksandra
AU  - Gasparovic, I.
AU  - Peterlin, B.
AU  - Klupka-Saric, I.
AU  - Živković, Maja
AU  - Starčević Čizmarević, Nada
AU  - Lovrecic, L.
AU  - Sinanovic, O.
AU  - Dinčić, Evica
AU  - Perkovic, O.
AU  - Rudolf, G.
AU  - Vidovic, M.
AU  - Stojković, Ljiljana S.
AU  - Lavtar, P.
AU  - Sehanovic, A.
AU  - Ristic, S.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2737
C3  - Multiple Sclerosis Journal
T1  - Angiotensin-converting enzyme I/D gene polymorphism in multiple sclerosis
VL  - 17
SP  - S115
EP  - S115
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2737
ER  - 

@conference{
author = "Stanković, Aleksandra and Gasparovic, I. and Peterlin, B. and Klupka-Saric, I. and Živković, Maja and Starčević Čizmarević, Nada and Lovrecic, L. and Sinanovic, O. and Dinčić, Evica and Perkovic, O. and Rudolf, G. and Vidovic, M. and Stojković, Ljiljana S. and Lavtar, P. and Sehanovic, A. and Ristic, S.",
year = "2011",
journal = "Multiple Sclerosis Journal",
title = "Angiotensin-converting enzyme I/D gene polymorphism in multiple sclerosis",
volume = "17",
pages = "S115-S115",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2737"
}

Stanković, A., Gasparovic, I., Peterlin, B., Klupka-Saric, I., Živković, M., Starčević Čizmarević, N., Lovrecic, L., Sinanovic, O., Dinčić, E., Perkovic, O., Rudolf, G., Vidovic, M., Stojković, L. S., Lavtar, P., Sehanovic, A.,& Ristic, S.. (2011). Angiotensin-converting enzyme I/D gene polymorphism in multiple sclerosis. in Multiple Sclerosis Journal, 17, S115-S115.
https://hdl.handle.net/21.15107/rcub_vinar_2737

Stanković A, Gasparovic I, Peterlin B, Klupka-Saric I, Živković M, Starčević Čizmarević N, Lovrecic L, Sinanovic O, Dinčić E, Perkovic O, Rudolf G, Vidovic M, Stojković LS, Lavtar P, Sehanovic A, Ristic S. Angiotensin-converting enzyme I/D gene polymorphism in multiple sclerosis. in Multiple Sclerosis Journal. 2011;17:S115-S115.
https://hdl.handle.net/21.15107/rcub_vinar_2737 .

Stanković, Aleksandra, Gasparovic, I., Peterlin, B., Klupka-Saric, I., Živković, Maja, Starčević Čizmarević, Nada, Lovrecic, L., Sinanovic, O., Dinčić, Evica, Perkovic, O., Rudolf, G., Vidovic, M., Stojković, Ljiljana S., Lavtar, P., Sehanovic, A., Ristic, S., "Angiotensin-converting enzyme I/D gene polymorphism in multiple sclerosis" in Multiple Sclerosis Journal, 17 (2011):S115-S115,
https://hdl.handle.net/21.15107/rcub_vinar_2737 .

TY  - CONF
AU  - Stojković, Ljiljana
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Živković, Maja
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2738
C3  - Multiple Sclerosis Journal
T1  - The association of CXCL16 A181V and I142T gene polymorphisms with multiple sclerosis
VL  - 17
SP  - S339
EP  - S339
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2738
ER  - 

@conference{
author = "Stojković, Ljiljana and Stanković, Aleksandra and Dinčić, Evica and Živković, Maja",
year = "2011",
journal = "Multiple Sclerosis Journal",
title = "The association of CXCL16 A181V and I142T gene polymorphisms with multiple sclerosis",
volume = "17",
pages = "S339-S339",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2738"
}

Stojković, L., Stanković, A., Dinčić, E.,& Živković, M.. (2011). The association of CXCL16 A181V and I142T gene polymorphisms with multiple sclerosis. in Multiple Sclerosis Journal, 17, S339-S339.
https://hdl.handle.net/21.15107/rcub_vinar_2738

Stojković L, Stanković A, Dinčić E, Živković M. The association of CXCL16 A181V and I142T gene polymorphisms with multiple sclerosis. in Multiple Sclerosis Journal. 2011;17:S339-S339.
https://hdl.handle.net/21.15107/rcub_vinar_2738 .

Stojković, Ljiljana, Stanković, Aleksandra, Dinčić, Evica, Živković, Maja, "The association of CXCL16 A181V and I142T gene polymorphisms with multiple sclerosis" in Multiple Sclerosis Journal, 17 (2011):S339-S339,
https://hdl.handle.net/21.15107/rcub_vinar_2738 .

TY  - CONF
AU  - Kolaković, Ana P.
AU  - Živković, Maja
AU  - Dinčić, Evica
AU  - Popović, Smiljana
AU  - Raičević, Ranko
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2010
PY  - 10 Supplement
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12791
AB  - Multiple  sclerosis  (MS)  is  a  complex  inflammatory,demyelinating disease of central nervous system (CNS). All the essen-tial components of the renin-angiotensin system (RAS) are presentedin  the  mammalian  brain.  The  angiotensin  II  (Ang  II),  biologicallyactive octapeptide is not only a vasoconstrictor, but also a pro-inflam-matory factor. Many of the classical and of the hypothetical functionsof brain Ang II are mediated by stimulation of AT1 receptors (AT1R).Brain AT2 receptors (AT2R) are highly expressed during development.In the adults, AT2R are restricted to areas predominantly involved inthe process of sensory information. The AT2R1332 A/G polymorph-ism was proposed to influence AT2R protein expression, and is themost studied polymorphism in this gene, in other diseases. Recently,the  striking  appearance  of  the  RAS  in  MS  brain  was  described.However, the role of AT2R remains to be clarified. Thus, the aim ofour  study  was  to  establish  if  there  is  an  association  between  AT2R1332 A/G gene polymorphism and predisposition of MS Methods:Subjected  group  consisted  of  122  female  and  70  malepatients with MS and 75 female and 50 male controls from populationof Serbia. Genotyping was done by PCR and restriction digestion withEcoRI enzyme.Results:The  genotype  and  allele  frequencies  for  AT2R1332A/Ggene  polymorphism  are  analyzed  separately  in  females  and  males,since this gene is located on X chromosome. We detected significantoverrepresentation of1332A/G AA genotype (OR 1.6, 95% CI:1.0–2.7, p<0.05) in female patients with MS compared to female controls.In hemizygous males we didn’t found any difference between patientsand controls.Conclusion:The  role  of  RAS  genes  in  MS  was  neglected  untilrecently.  Than,  it  was  shown  that  the  role  of  RAS  in  the  CNS  isbeyond  the  regulation  of  cardiovascular  function.  Until  now  AT2R(1332A/G)  gene  polymorphism  was widely studied  and associatedwith hypertension and other vascular disease. Until now, there wereno studies concerning role of Ang II receptor polymorphisms in MS.This study  suggest  possible  role  of  AT2R in  MS.  Further  studies areneeded to elucidate this result.
C3  - Multiple Sclerosis Journal
T1  - Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis
VL  - 16
SP  - S80
EP  - S81
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12791
ER  - 

@conference{
author = "Kolaković, Ana P. and Živković, Maja and Dinčić, Evica and Popović, Smiljana and Raičević, Ranko and Alavantić, Dragan and Stanković, Aleksandra",
year = "2010, 10 Supplement",
abstract = "Multiple  sclerosis  (MS)  is  a  complex  inflammatory,demyelinating disease of central nervous system (CNS). All the essen-tial components of the renin-angiotensin system (RAS) are presentedin  the  mammalian  brain.  The  angiotensin  II  (Ang  II),  biologicallyactive octapeptide is not only a vasoconstrictor, but also a pro-inflam-matory factor. Many of the classical and of the hypothetical functionsof brain Ang II are mediated by stimulation of AT1 receptors (AT1R).Brain AT2 receptors (AT2R) are highly expressed during development.In the adults, AT2R are restricted to areas predominantly involved inthe process of sensory information. The AT2R1332 A/G polymorph-ism was proposed to influence AT2R protein expression, and is themost studied polymorphism in this gene, in other diseases. Recently,the  striking  appearance  of  the  RAS  in  MS  brain  was  described.However, the role of AT2R remains to be clarified. Thus, the aim ofour  study  was  to  establish  if  there  is  an  association  between  AT2R1332 A/G gene polymorphism and predisposition of MS Methods:Subjected  group  consisted  of  122  female  and  70  malepatients with MS and 75 female and 50 male controls from populationof Serbia. Genotyping was done by PCR and restriction digestion withEcoRI enzyme.Results:The  genotype  and  allele  frequencies  for  AT2R1332A/Ggene  polymorphism  are  analyzed  separately  in  females  and  males,since this gene is located on X chromosome. We detected significantoverrepresentation of1332A/G AA genotype (OR 1.6, 95% CI:1.0–2.7, p<0.05) in female patients with MS compared to female controls.In hemizygous males we didn’t found any difference between patientsand controls.Conclusion:The  role  of  RAS  genes  in  MS  was  neglected  untilrecently.  Than,  it  was  shown  that  the  role  of  RAS  in  the  CNS  isbeyond  the  regulation  of  cardiovascular  function.  Until  now  AT2R(1332A/G)  gene  polymorphism  was widely studied  and associatedwith hypertension and other vascular disease. Until now, there wereno studies concerning role of Ang II receptor polymorphisms in MS.This study  suggest  possible  role  of  AT2R in  MS.  Further  studies areneeded to elucidate this result.",
journal = "Multiple Sclerosis Journal",
title = "Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis",
volume = "16",
pages = "S80-S81",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12791"
}

Kolaković, A. P., Živković, M., Dinčić, E., Popović, S., Raičević, R., Alavantić, D.,& Stanković, A.. (2010). Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis. in Multiple Sclerosis Journal, 16, S80-S81.
https://hdl.handle.net/21.15107/rcub_vinar_12791

Kolaković AP, Živković M, Dinčić E, Popović S, Raičević R, Alavantić D, Stanković A. Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis. in Multiple Sclerosis Journal. 2010;16:S80-S81.
https://hdl.handle.net/21.15107/rcub_vinar_12791 .

Kolaković, Ana P., Živković, Maja, Dinčić, Evica, Popović, Smiljana, Raičević, Ranko, Alavantić, Dragan, Stanković, Aleksandra, "Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis" in Multiple Sclerosis Journal, 16 (2010):S80-S81,
https://hdl.handle.net/21.15107/rcub_vinar_12791 .

TY  - JOUR
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Ristić, Smiljana
AU  - Lovrečić, Luca
AU  - Starčević Čizmarević, Nada
AU  - Đurić, Tamara
AU  - Sepčić, Juraj
AU  - Kapović, Miljenko
AU  - Raičević, Ranko
AU  - Peterlin, Borut
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4004
AB  - The interleukin 7 receptor alpha single nucleotide polymorphism rs6897932 was identified as a multiple sclerosis susceptibility-modifying polymorphism in genome-wide and gene scan studies, mainly in populations in western countries. The aim of this study was to investigate the association of interleukin 7 receptor alpha rs6897932 with multiple sclerosis in populations from the Western Balkans: Serbia, Croatia, and Slovenia. A total of 678 unrelated white patients and 597 unrelated, ethnically matched healthy controls were included in the study. Genotyping was performed by real-time polymerase chain reaction. We found no significant difference in genotype or allele frequencies between controls and patients with multiple sclerosis either separately in Serbian, Croatian, and Slovenian populations or in the whole sample from the Western Balkans. The odds ratio for multiple sclerosis in this study was 1.04 (0.86-1.25) for the C allele. It is known that demographic as well as environmental factors have a substantial role in multiple sclerosis development, as well as population genetic background. The results of this study indicate that other types of genome variants should be required for the development and/or progression of multiple sclerosis, which may vary among populations.
T2  - Multiple Sclerosis Journal
T1  - Interleukin 7 receptor alpha polymorphism rs6897932 and susceptibility to multiple sclerosis in the Western Balkans
VL  - 16
IS  - 5
SP  - 533
EP  - 536
DO  - 10.1177/1352458509360548
ER  - 

@article{
author = "Stanković, Aleksandra and Dinčić, Evica and Ristić, Smiljana and Lovrečić, Luca and Starčević Čizmarević, Nada and Đurić, Tamara and Sepčić, Juraj and Kapović, Miljenko and Raičević, Ranko and Peterlin, Borut and Alavantić, Dragan and Živković, Maja",
year = "2010",
abstract = "The interleukin 7 receptor alpha single nucleotide polymorphism rs6897932 was identified as a multiple sclerosis susceptibility-modifying polymorphism in genome-wide and gene scan studies, mainly in populations in western countries. The aim of this study was to investigate the association of interleukin 7 receptor alpha rs6897932 with multiple sclerosis in populations from the Western Balkans: Serbia, Croatia, and Slovenia. A total of 678 unrelated white patients and 597 unrelated, ethnically matched healthy controls were included in the study. Genotyping was performed by real-time polymerase chain reaction. We found no significant difference in genotype or allele frequencies between controls and patients with multiple sclerosis either separately in Serbian, Croatian, and Slovenian populations or in the whole sample from the Western Balkans. The odds ratio for multiple sclerosis in this study was 1.04 (0.86-1.25) for the C allele. It is known that demographic as well as environmental factors have a substantial role in multiple sclerosis development, as well as population genetic background. The results of this study indicate that other types of genome variants should be required for the development and/or progression of multiple sclerosis, which may vary among populations.",
journal = "Multiple Sclerosis Journal",
title = "Interleukin 7 receptor alpha polymorphism rs6897932 and susceptibility to multiple sclerosis in the Western Balkans",
volume = "16",
number = "5",
pages = "533-536",
doi = "10.1177/1352458509360548"
}

Stanković, A., Dinčić, E., Ristić, S., Lovrečić, L., Starčević Čizmarević, N., Đurić, T., Sepčić, J., Kapović, M., Raičević, R., Peterlin, B., Alavantić, D.,& Živković, M.. (2010). Interleukin 7 receptor alpha polymorphism rs6897932 and susceptibility to multiple sclerosis in the Western Balkans. in Multiple Sclerosis Journal, 16(5), 533-536.
https://doi.org/10.1177/1352458509360548

Stanković A, Dinčić E, Ristić S, Lovrečić L, Starčević Čizmarević N, Đurić T, Sepčić J, Kapović M, Raičević R, Peterlin B, Alavantić D, Živković M. Interleukin 7 receptor alpha polymorphism rs6897932 and susceptibility to multiple sclerosis in the Western Balkans. in Multiple Sclerosis Journal. 2010;16(5):533-536.
doi:10.1177/1352458509360548 .

Stanković, Aleksandra, Dinčić, Evica, Ristić, Smiljana, Lovrečić, Luca, Starčević Čizmarević, Nada, Đurić, Tamara, Sepčić, Juraj, Kapović, Miljenko, Raičević, Ranko, Peterlin, Borut, Alavantić, Dragan, Živković, Maja, "Interleukin 7 receptor alpha polymorphism rs6897932 and susceptibility to multiple sclerosis in the Western Balkans" in Multiple Sclerosis Journal, 16, no. 5 (2010):533-536,
https://doi.org/10.1177/1352458509360548 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Popović, Milan
AU  - Popovic, Smiljana
AU  - Raičević, Ranko
AU  - Alavantić, Dragan
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3771
AB  - Background: Recently a high-resolution HLA and SNP map was defined and the analysis provided informative tag SNPs that capture much of the common variation in the MHC region. This concept enables detection of smaller number of SNPs, making it surrogate markers for haplotype associated with certain disease. The SNP rs3135388 was proposed as a tagging SNP for DRB1*1501/DQB1*0602 alleles, associated with MS. The aim of the study was to investigate the HLA rs3135388 genotypes in association with MS in patients from Serbia. Methods: Two hundred sixty nine consecutive patients from Serbia with relapse-remitting and secondary progressive MS were recruited for the study. Genomic DNA was isolated from peripheral blood cells. We designed the TaqMan assay for high-throughput genotyping of HLA rs3135388 on 7500 Real-Time PCR System. Results: We found significantly higher frequency of rs3135388 A allele carriers in MS patients compared to controls (p LT 0.001, chi(2)). In our population the carriers of one A allele had adjusted OR 2.09 (95% CI 1.41-3.09, p LT 0.001) for MS susceptibility. Conclusion: We assessed significant association of rs3135388 A allele carriership with MS in patients from Serbia. This HLA-DRB1*1501 surrogate marker is useful in association studies in MS. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Clinica Chimica Acta
T1  - The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR
VL  - 406
IS  - 1-2
SP  - 27
EP  - 30
DO  - 10.1016/j.cca.2009.05.004
ER  - 

@article{
author = "Živković, Maja and Stanković, Aleksandra and Dinčić, Evica and Popović, Milan and Popovic, Smiljana and Raičević, Ranko and Alavantić, Dragan",
year = "2009",
abstract = "Background: Recently a high-resolution HLA and SNP map was defined and the analysis provided informative tag SNPs that capture much of the common variation in the MHC region. This concept enables detection of smaller number of SNPs, making it surrogate markers for haplotype associated with certain disease. The SNP rs3135388 was proposed as a tagging SNP for DRB1*1501/DQB1*0602 alleles, associated with MS. The aim of the study was to investigate the HLA rs3135388 genotypes in association with MS in patients from Serbia. Methods: Two hundred sixty nine consecutive patients from Serbia with relapse-remitting and secondary progressive MS were recruited for the study. Genomic DNA was isolated from peripheral blood cells. We designed the TaqMan assay for high-throughput genotyping of HLA rs3135388 on 7500 Real-Time PCR System. Results: We found significantly higher frequency of rs3135388 A allele carriers in MS patients compared to controls (p LT 0.001, chi(2)). In our population the carriers of one A allele had adjusted OR 2.09 (95% CI 1.41-3.09, p LT 0.001) for MS susceptibility. Conclusion: We assessed significant association of rs3135388 A allele carriership with MS in patients from Serbia. This HLA-DRB1*1501 surrogate marker is useful in association studies in MS. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Clinica Chimica Acta",
title = "The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR",
volume = "406",
number = "1-2",
pages = "27-30",
doi = "10.1016/j.cca.2009.05.004"
}

Živković, M., Stanković, A., Dinčić, E., Popović, M., Popovic, S., Raičević, R.,& Alavantić, D.. (2009). The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR. in Clinica Chimica Acta, 406(1-2), 27-30.
https://doi.org/10.1016/j.cca.2009.05.004

Živković M, Stanković A, Dinčić E, Popović M, Popovic S, Raičević R, Alavantić D. The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR. in Clinica Chimica Acta. 2009;406(1-2):27-30.
doi:10.1016/j.cca.2009.05.004 .

Živković, Maja, Stanković, Aleksandra, Dinčić, Evica, Popović, Milan, Popovic, Smiljana, Raičević, Ranko, Alavantić, Dragan, "The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR" in Clinica Chimica Acta, 406, no. 1-2 (2009):27-30,
https://doi.org/10.1016/j.cca.2009.05.004 . .

TY  - JOUR
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Raičević, Ranko
AU  - Alavantić, Dragan
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3237
AB  - Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in remodeling of the extracellular matrix. MMPs are suggested to play a role in the influx of inflammatory cells into the CNS, disruption of the blood brain barrier, and to degrade myelin in vitro. In this study, we have investigated the possible association of MMP-3 5A/6A gene polymorphism with MS susceptibility and/or severity in patients from Serbia. A total of 184 MS patients (150 RR, 34 SP) and 236 controls have been studied. Results show that the distribution of MMP-3 5A/6A genotype frequencies between NIS patients and controls were not significantly different. In bout onset patients, carriers of MMP-3 6A/6A genotype had significantly higher mean MSSS values compared to the carriers of 5A allele (6.29 +/- 1.89 vs. 5.29 +/- 2.62, respectively, ANCOVA, p=0.01 Scheffe post-hoc test). In conclusion, our results indicate association of MMP-3 6A/6A genotype with significantly higher mean MSSS values. Thus, the obtained results suggest that it should be carefully considered during follow up of patients with MS. Further genetic and functional studies are needed to resolve the complex role of MMPs and their tissue inhibitors in NIS pathology and/or regeneration. (C) 2007 Elsevier B.V. All rights reserved.
T2  - Journal of the Neurological Sciences
T1  - Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia
VL  - 267
IS  - 1-2
SP  - 62
EP  - 65
DO  - 10.1016/j.jns.2007.09.037
ER  - 

@article{
author = "Đurić, Tamara and Živković, Maja and Stanković, Aleksandra and Dinčić, Evica and Raičević, Ranko and Alavantić, Dragan",
year = "2008",
abstract = "Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in remodeling of the extracellular matrix. MMPs are suggested to play a role in the influx of inflammatory cells into the CNS, disruption of the blood brain barrier, and to degrade myelin in vitro. In this study, we have investigated the possible association of MMP-3 5A/6A gene polymorphism with MS susceptibility and/or severity in patients from Serbia. A total of 184 MS patients (150 RR, 34 SP) and 236 controls have been studied. Results show that the distribution of MMP-3 5A/6A genotype frequencies between NIS patients and controls were not significantly different. In bout onset patients, carriers of MMP-3 6A/6A genotype had significantly higher mean MSSS values compared to the carriers of 5A allele (6.29 +/- 1.89 vs. 5.29 +/- 2.62, respectively, ANCOVA, p=0.01 Scheffe post-hoc test). In conclusion, our results indicate association of MMP-3 6A/6A genotype with significantly higher mean MSSS values. Thus, the obtained results suggest that it should be carefully considered during follow up of patients with MS. Further genetic and functional studies are needed to resolve the complex role of MMPs and their tissue inhibitors in NIS pathology and/or regeneration. (C) 2007 Elsevier B.V. All rights reserved.",
journal = "Journal of the Neurological Sciences",
title = "Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia",
volume = "267",
number = "1-2",
pages = "62-65",
doi = "10.1016/j.jns.2007.09.037"
}

Đurić, T., Živković, M., Stanković, A., Dinčić, E., Raičević, R.,& Alavantić, D.. (2008). Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia. in Journal of the Neurological Sciences, 267(1-2), 62-65.
https://doi.org/10.1016/j.jns.2007.09.037

Đurić T, Živković M, Stanković A, Dinčić E, Raičević R, Alavantić D. Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia. in Journal of the Neurological Sciences. 2008;267(1-2):62-65.
doi:10.1016/j.jns.2007.09.037 .

Đurić, Tamara, Živković, Maja, Stanković, Aleksandra, Dinčić, Evica, Raičević, Ranko, Alavantić, Dragan, "Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia" in Journal of the Neurological Sciences, 267, no. 1-2 (2008):62-65,
https://doi.org/10.1016/j.jns.2007.09.037 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Dinčić, Evica
AU  - Raičević, Ranko
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3299
AB  - Matrix metalloproteinase-9 (MMP-9) is suggested to play a role in MS by mediating T cell migration across subendothelial basement membrane and by contribution to myelin breakdown. We studied the association of MMP-9 -1562 C/T gene polymorphisms with MS susceptibility and severity in 187 patients from Serbia. The significant decrease in T allele carriership (p=0.01), was found in female NIS patients, In addition, a trend toward lower MSSS in T allele carriers was noticed (CC, mean 5.7 +/- 2.5 vs. CT+TT, mean 4.9 +/- 2.5). Further studies in different populations are needed to resolve the potential influence of MMP-9 gene polymorphism on MS. (C) 2007 Elsevier B.V. All rights reserved.
T2  - Journal of Neuroimmunology
T1  - Matrix metalloproteinase-9-1562 C/T gene polymorphism in Serbian patients with multiple sclerosis
VL  - 189
IS  - 1-2
SP  - 147
EP  - 150
DO  - 10.1016/j.jneuroim.2007.06.022
ER  - 

@article{
author = "Živković, Maja and Đurić, Tamara and Dinčić, Evica and Raičević, Ranko and Alavantić, Dragan and Stanković, Aleksandra",
year = "2007",
abstract = "Matrix metalloproteinase-9 (MMP-9) is suggested to play a role in MS by mediating T cell migration across subendothelial basement membrane and by contribution to myelin breakdown. We studied the association of MMP-9 -1562 C/T gene polymorphisms with MS susceptibility and severity in 187 patients from Serbia. The significant decrease in T allele carriership (p=0.01), was found in female NIS patients, In addition, a trend toward lower MSSS in T allele carriers was noticed (CC, mean 5.7 +/- 2.5 vs. CT+TT, mean 4.9 +/- 2.5). Further studies in different populations are needed to resolve the potential influence of MMP-9 gene polymorphism on MS. (C) 2007 Elsevier B.V. All rights reserved.",
journal = "Journal of Neuroimmunology",
title = "Matrix metalloproteinase-9-1562 C/T gene polymorphism in Serbian patients with multiple sclerosis",
volume = "189",
number = "1-2",
pages = "147-150",
doi = "10.1016/j.jneuroim.2007.06.022"
}

Živković, M., Đurić, T., Dinčić, E., Raičević, R., Alavantić, D.,& Stanković, A.. (2007). Matrix metalloproteinase-9-1562 C/T gene polymorphism in Serbian patients with multiple sclerosis. in Journal of Neuroimmunology, 189(1-2), 147-150.
https://doi.org/10.1016/j.jneuroim.2007.06.022

Živković M, Đurić T, Dinčić E, Raičević R, Alavantić D, Stanković A. Matrix metalloproteinase-9-1562 C/T gene polymorphism in Serbian patients with multiple sclerosis. in Journal of Neuroimmunology. 2007;189(1-2):147-150.
doi:10.1016/j.jneuroim.2007.06.022 .

Živković, Maja, Đurić, Tamara, Dinčić, Evica, Raičević, Ranko, Alavantić, Dragan, Stanković, Aleksandra, "Matrix metalloproteinase-9-1562 C/T gene polymorphism in Serbian patients with multiple sclerosis" in Journal of Neuroimmunology, 189, no. 1-2 (2007):147-150,
https://doi.org/10.1016/j.jneuroim.2007.06.022 . .