TY  - JOUR
AU  - Stanojević, Boban
AU  - Osiowy, Carla
AU  - Schaefer, Stephan
AU  - Bojovic, Ksenija
AU  - Blagojevic, Jelena
AU  - Nesic, Milica
AU  - Yamashita, Shunichi
AU  - Stamenković, Gorana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4450
AB  - Hepatitis B virus (HBV) is classified into 8 genotypes with distinct geographical distribution. Genotype D (HBV/D) has the widest distribution area and is comprised of 7 subgenotypes. Subgenotypes D1, D2 and D3 appear worldwide, while D4-D7 have a more restricted distribution. Within the Mediterranean area, HBV/D and subgenotype D3 are the most prevalent. The purpose of this study was to characterize the full genome of Serbian HBV/D3 isolates by comparison and phylogenetic analysis with HBV/D3 sequences (66 samples) found in GeneBank/DDBJ databases from different parts of the world. Isolates were obtained from three patients diagnosed with chronic hepatitis B (HBsAg +). All three isolates have two very rare nucleotide substitutions, A929T and T150A, which indicate the same ancestor. Phylogenetic analysis of HBV/D3 genome sequences throughout the world follows an ethno-geographical origin of isolates with rare exceptions, which could be explained by human travelling and migration. The geographically close but ethnically different Serbian and Italian isolates clustered in the same subnode, and on a common branch with strains from Northern Canada. To test the apparently close HBV phylogenetic relationship between completely separated patients from Serbia and Northern Canada we analyzed in depth a 440 bp region of the HBsAg from Canadian (n = 73) and Serbian (n = 70) isolates. The constructed parsimony tree revealed that strains from Serbia and Northern Canada fell along the same branch which indicates independent evolution within regions of each country, Considering that HBsAg sequence has limited variability for phylogenetic analyses, our hypothesis needs further confirmation with more HBV complete genome sequences. (C) 2011 Elsevier B.V. All rights reserved,
T2  - Infection Genetics and Evolution
T1  - Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia
VL  - 11
IS  - 6
SP  - 1475
EP  - 1480
DO  - 10.1016/j.meegid.2011.05.004
ER  - 

@article{
author = "Stanojević, Boban and Osiowy, Carla and Schaefer, Stephan and Bojovic, Ksenija and Blagojevic, Jelena and Nesic, Milica and Yamashita, Shunichi and Stamenković, Gorana",
year = "2011",
abstract = "Hepatitis B virus (HBV) is classified into 8 genotypes with distinct geographical distribution. Genotype D (HBV/D) has the widest distribution area and is comprised of 7 subgenotypes. Subgenotypes D1, D2 and D3 appear worldwide, while D4-D7 have a more restricted distribution. Within the Mediterranean area, HBV/D and subgenotype D3 are the most prevalent. The purpose of this study was to characterize the full genome of Serbian HBV/D3 isolates by comparison and phylogenetic analysis with HBV/D3 sequences (66 samples) found in GeneBank/DDBJ databases from different parts of the world. Isolates were obtained from three patients diagnosed with chronic hepatitis B (HBsAg +). All three isolates have two very rare nucleotide substitutions, A929T and T150A, which indicate the same ancestor. Phylogenetic analysis of HBV/D3 genome sequences throughout the world follows an ethno-geographical origin of isolates with rare exceptions, which could be explained by human travelling and migration. The geographically close but ethnically different Serbian and Italian isolates clustered in the same subnode, and on a common branch with strains from Northern Canada. To test the apparently close HBV phylogenetic relationship between completely separated patients from Serbia and Northern Canada we analyzed in depth a 440 bp region of the HBsAg from Canadian (n = 73) and Serbian (n = 70) isolates. The constructed parsimony tree revealed that strains from Serbia and Northern Canada fell along the same branch which indicates independent evolution within regions of each country, Considering that HBsAg sequence has limited variability for phylogenetic analyses, our hypothesis needs further confirmation with more HBV complete genome sequences. (C) 2011 Elsevier B.V. All rights reserved,",
journal = "Infection Genetics and Evolution",
title = "Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia",
volume = "11",
number = "6",
pages = "1475-1480",
doi = "10.1016/j.meegid.2011.05.004"
}

Stanojević, B., Osiowy, C., Schaefer, S., Bojovic, K., Blagojevic, J., Nesic, M., Yamashita, S.,& Stamenković, G.. (2011). Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia. in Infection Genetics and Evolution, 11(6), 1475-1480.
https://doi.org/10.1016/j.meegid.2011.05.004

Stanojević B, Osiowy C, Schaefer S, Bojovic K, Blagojevic J, Nesic M, Yamashita S, Stamenković G. Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia. in Infection Genetics and Evolution. 2011;11(6):1475-1480.
doi:10.1016/j.meegid.2011.05.004 .

Stanojević, Boban, Osiowy, Carla, Schaefer, Stephan, Bojovic, Ksenija, Blagojevic, Jelena, Nesic, Milica, Yamashita, Shunichi, Stamenković, Gorana, "Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia" in Infection Genetics and Evolution, 11, no. 6 (2011):1475-1480,
https://doi.org/10.1016/j.meegid.2011.05.004 . .

TY  - JOUR
AU  - Tanić, Nikola
AU  - Stanojević, Boban
AU  - Tanić, Nikola
AU  - Schaefer, Stephan
AU  - Niesters, Hubert G. M.
AU  - Božić, Milena
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3789
AB  - Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Journal of Virological Methods
T1  - Concurrent quantitation of the A and D genotypes of hepatitis B virus
VL  - 161
IS  - 2
SP  - 265
EP  - 270
DO  - 10.1016/j.jviromet.2009.06.022
ER  - 

@article{
author = "Tanić, Nikola and Stanojević, Boban and Tanić, Nikola and Schaefer, Stephan and Niesters, Hubert G. M. and Božić, Milena and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Journal of Virological Methods",
title = "Concurrent quantitation of the A and D genotypes of hepatitis B virus",
volume = "161",
number = "2",
pages = "265-270",
doi = "10.1016/j.jviromet.2009.06.022"
}

Tanić, N., Stanojević, B., Tanić, N., Schaefer, S., Niesters, H. G. M., Božić, M.,& Dimitrijević, B. B.. (2009). Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods, 161(2), 265-270.
https://doi.org/10.1016/j.jviromet.2009.06.022

Tanić N, Stanojević B, Tanić N, Schaefer S, Niesters HGM, Božić M, Dimitrijević BB. Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods. 2009;161(2):265-270.
doi:10.1016/j.jviromet.2009.06.022 .

Tanić, Nikola, Stanojević, Boban, Tanić, Nikola, Schaefer, Stephan, Niesters, Hubert G. M., Božić, Milena, Dimitrijević, Bogomir B., "Concurrent quantitation of the A and D genotypes of hepatitis B virus" in Journal of Virological Methods, 161, no. 2 (2009):265-270,
https://doi.org/10.1016/j.jviromet.2009.06.022 . .