Pajović, Jelena D.

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orcid::0000-0002-3644-0118
  • Pajović, Jelena D. (7)
  • Pajović, Jelena (1)

Author's Bibliography

Fluorescence microscopy and photodielectric characterization studies of the composite films of polyvinyl alcohol and tryptophan functionalized silver nanoparticles

Božanić, Dušan K.; Dojčilović, Radovan; Pajović, Jelena D.; Tošić, Dragana; Dudić, Duško; Réfrégiers, Matthieu; Đoković, Vladimir

(2022)

TY  - JOUR
AU  - Božanić, Dušan K.
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Tošić, Dragana
AU  - Dudić, Duško
AU  - Réfrégiers, Matthieu
AU  - Đoković, Vladimir
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10076
AB  - Fluorescent nanocomposite films were prepared by solution mixing of polyvinyl alcohol (PVA) and tryptophan functionalized silver (AgTrp) nanoparticles. Synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging was used to follow the distribution of AgTrp nanoparticles within the polymer matrix. Pronounced growth of dendrite-like structures in the presence of AgTrp nanoparticles was confirmed by bright field and fluorescence imaging as well as with atomic force microscopies. Both tryptophan and AgTrp nanoparticles influence thermal properties of the matrix. Differential scanning calorimetry measurements showed that they reduce crystallinity and significantly increase the glass transition temperature of PVA (in the case of PVA-AgTrp film the Tg increases by ~20 °C). Dielectric properties of the films were studied under two different conditions (dark and illumination). The illumination with 250 nm light induces a significant increase in conductance of the PVA-AgTrp nanocomposite. The relative changes, with respect to the conductance measured in the dark, were almost ~200%.
T2  - Colloids and Surfaces A: Physicochemical and Engineering Aspects
T1  - Fluorescence microscopy and photodielectric characterization studies of the composite films of polyvinyl alcohol and tryptophan functionalized silver nanoparticles
VL  - 634
SP  - 128050
DO  - 10.1016/j.colsurfa.2021.128050
ER  - 
@article{
author = "Božanić, Dušan K. and Dojčilović, Radovan and Pajović, Jelena D. and Tošić, Dragana and Dudić, Duško and Réfrégiers, Matthieu and Đoković, Vladimir",
year = "2022",
abstract = "Fluorescent nanocomposite films were prepared by solution mixing of polyvinyl alcohol (PVA) and tryptophan functionalized silver (AgTrp) nanoparticles. Synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging was used to follow the distribution of AgTrp nanoparticles within the polymer matrix. Pronounced growth of dendrite-like structures in the presence of AgTrp nanoparticles was confirmed by bright field and fluorescence imaging as well as with atomic force microscopies. Both tryptophan and AgTrp nanoparticles influence thermal properties of the matrix. Differential scanning calorimetry measurements showed that they reduce crystallinity and significantly increase the glass transition temperature of PVA (in the case of PVA-AgTrp film the Tg increases by ~20 °C). Dielectric properties of the films were studied under two different conditions (dark and illumination). The illumination with 250 nm light induces a significant increase in conductance of the PVA-AgTrp nanocomposite. The relative changes, with respect to the conductance measured in the dark, were almost ~200%.",
journal = "Colloids and Surfaces A: Physicochemical and Engineering Aspects",
title = "Fluorescence microscopy and photodielectric characterization studies of the composite films of polyvinyl alcohol and tryptophan functionalized silver nanoparticles",
volume = "634",
pages = "128050",
doi = "10.1016/j.colsurfa.2021.128050"
}
Božanić, D. K., Dojčilović, R., Pajović, J. D., Tošić, D., Dudić, D., Réfrégiers, M.,& Đoković, V.. (2022). Fluorescence microscopy and photodielectric characterization studies of the composite films of polyvinyl alcohol and tryptophan functionalized silver nanoparticles. in Colloids and Surfaces A: Physicochemical and Engineering Aspects, 634, 128050.
https://doi.org/10.1016/j.colsurfa.2021.128050
Božanić DK, Dojčilović R, Pajović JD, Tošić D, Dudić D, Réfrégiers M, Đoković V. Fluorescence microscopy and photodielectric characterization studies of the composite films of polyvinyl alcohol and tryptophan functionalized silver nanoparticles. in Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2022;634:128050.
doi:10.1016/j.colsurfa.2021.128050 .
Božanić, Dušan K., Dojčilović, Radovan, Pajović, Jelena D., Tošić, Dragana, Dudić, Duško, Réfrégiers, Matthieu, Đoković, Vladimir, "Fluorescence microscopy and photodielectric characterization studies of the composite films of polyvinyl alcohol and tryptophan functionalized silver nanoparticles" in Colloids and Surfaces A: Physicochemical and Engineering Aspects, 634 (2022):128050,
https://doi.org/10.1016/j.colsurfa.2021.128050 . .
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Velocity Map Imaging VUV Angle-Resolved Photoemission on Isolated Nanosystems: Case of Gold Nanoparticles

Božanić, Dušan K.; Garcia, Gustavo A.; Sublemontier, Olivier; Pajović, Jelena; Đoković, Vladimir; Nahon, Laurent

(2020)

TY  - JOUR
AU  - Božanić, Dušan K.
AU  - Garcia, Gustavo A.
AU  - Sublemontier, Olivier
AU  - Pajović, Jelena
AU  - Đoković, Vladimir
AU  - Nahon, Laurent
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9727
AB  - We present an angle-resolved photoelectron spectroscopy study on isolated gold nanoparticles in the photon energy range between 6 and 12 eV performed at the DESIRS beamline of Synchrotron SOLEIL with a velocity map imaging spectrometer. The data are reported for polydisperse gold aerosols formed from hydrocolloids of bare and citrate-capped primary nanoparticles. The aerosols were brought to interact with the synchrotron radiation via a high-performance aerodynamic lens system developed for this study. The electronic structure of the aerosols was elucidated by threshold photoemission spectroscopy, which showed excellent agreement with the theoretical density of states. The photoionization cross sections of isolated gold particles were derived in the ∼104 to 106 Mb range between 8 and 12 eV. We introduce a new methodology to extract all of the photoemission observables from the electron images as a function of electron energy, including photoelectron spectrum, anisotropy parameter b2, and asymmetry parameter, α(E), which describes the shadowing asymmetry along the radiation propagation axis. The results show that electron energy distribution depends on the procedure used in the synthesis of the nanoparticles and on the ligand concentration. The dependence of the asymmetry parameter on electron energy along the valence band is rationalized through theoretical modeling in terms of the electric field distribution inside the particle and the losses due to inelastic scattering.
T2  - The Journal of Physical Chemistry C
T1  - Velocity Map Imaging VUV Angle-Resolved Photoemission on Isolated Nanosystems: Case of Gold Nanoparticles
VL  - 124
IS  - 44
SP  - 24500
EP  - 24512
DO  - 10.1021/acs.jpcc.0c08152
ER  - 
@article{
author = "Božanić, Dušan K. and Garcia, Gustavo A. and Sublemontier, Olivier and Pajović, Jelena and Đoković, Vladimir and Nahon, Laurent",
year = "2020",
abstract = "We present an angle-resolved photoelectron spectroscopy study on isolated gold nanoparticles in the photon energy range between 6 and 12 eV performed at the DESIRS beamline of Synchrotron SOLEIL with a velocity map imaging spectrometer. The data are reported for polydisperse gold aerosols formed from hydrocolloids of bare and citrate-capped primary nanoparticles. The aerosols were brought to interact with the synchrotron radiation via a high-performance aerodynamic lens system developed for this study. The electronic structure of the aerosols was elucidated by threshold photoemission spectroscopy, which showed excellent agreement with the theoretical density of states. The photoionization cross sections of isolated gold particles were derived in the ∼104 to 106 Mb range between 8 and 12 eV. We introduce a new methodology to extract all of the photoemission observables from the electron images as a function of electron energy, including photoelectron spectrum, anisotropy parameter b2, and asymmetry parameter, α(E), which describes the shadowing asymmetry along the radiation propagation axis. The results show that electron energy distribution depends on the procedure used in the synthesis of the nanoparticles and on the ligand concentration. The dependence of the asymmetry parameter on electron energy along the valence band is rationalized through theoretical modeling in terms of the electric field distribution inside the particle and the losses due to inelastic scattering.",
journal = "The Journal of Physical Chemistry C",
title = "Velocity Map Imaging VUV Angle-Resolved Photoemission on Isolated Nanosystems: Case of Gold Nanoparticles",
volume = "124",
number = "44",
pages = "24500-24512",
doi = "10.1021/acs.jpcc.0c08152"
}
Božanić, D. K., Garcia, G. A., Sublemontier, O., Pajović, J., Đoković, V.,& Nahon, L.. (2020). Velocity Map Imaging VUV Angle-Resolved Photoemission on Isolated Nanosystems: Case of Gold Nanoparticles. in The Journal of Physical Chemistry C, 124(44), 24500-24512.
https://doi.org/10.1021/acs.jpcc.0c08152
Božanić DK, Garcia GA, Sublemontier O, Pajović J, Đoković V, Nahon L. Velocity Map Imaging VUV Angle-Resolved Photoemission on Isolated Nanosystems: Case of Gold Nanoparticles. in The Journal of Physical Chemistry C. 2020;124(44):24500-24512.
doi:10.1021/acs.jpcc.0c08152 .
Božanić, Dušan K., Garcia, Gustavo A., Sublemontier, Olivier, Pajović, Jelena, Đoković, Vladimir, Nahon, Laurent, "Velocity Map Imaging VUV Angle-Resolved Photoemission on Isolated Nanosystems: Case of Gold Nanoparticles" in The Journal of Physical Chemistry C, 124, no. 44 (2020):24500-24512,
https://doi.org/10.1021/acs.jpcc.0c08152 . .
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DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells

Dojčilović, Radovan; Pajović, Jelena D.; Božanić, Dušan K.; Jović, Nataša G.; Pavlović, Vera P.; Pavlović, Vladimir B.; Lenhardt Acković, Lea; Zeković, Ivana Lj.; Dramićanin, Miroslav; Kaščakova, Slavka; Refregiers, Matthieu; Rašić, Goran; Vlahović, Branislav; Đoković, Vladimir

(2018)

TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Jović, Nataša G.
AU  - Pavlović, Vera P.
AU  - Pavlović, Vladimir B.
AU  - Lenhardt Acković, Lea
AU  - Zeković, Ivana Lj.
AU  - Dramićanin, Miroslav
AU  - Kaščakova, Slavka
AU  - Refregiers, Matthieu
AU  - Rašić, Goran
AU  - Vlahović, Branislav
AU  - Đoković, Vladimir
PY  - 2018
UR  - http://stacks.iop.org/2053-1583/5/i=4/a=045019?key=crossref.f455f07bdfb3469c8077963ff0f38f95
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7909
AB  - The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. © 2018 IOP Publishing Ltd.
T2  - 2D Materials
T1  - DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells
VL  - 5
IS  - 4
SP  - 045019
DO  - 10.1088/2053-1583/aad72b
ER  - 
@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Jović, Nataša G. and Pavlović, Vera P. and Pavlović, Vladimir B. and Lenhardt Acković, Lea and Zeković, Ivana Lj. and Dramićanin, Miroslav and Kaščakova, Slavka and Refregiers, Matthieu and Rašić, Goran and Vlahović, Branislav and Đoković, Vladimir",
year = "2018",
abstract = "The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. © 2018 IOP Publishing Ltd.",
journal = "2D Materials",
title = "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells",
volume = "5",
number = "4",
pages = "045019",
doi = "10.1088/2053-1583/aad72b"
}
Dojčilović, R., Pajović, J. D., Božanić, D. K., Jović, N. G., Pavlović, V. P., Pavlović, V. B., Lenhardt Acković, L., Zeković, I. Lj., Dramićanin, M., Kaščakova, S., Refregiers, M., Rašić, G., Vlahović, B.,& Đoković, V.. (2018). DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials, 5(4), 045019.
https://doi.org/10.1088/2053-1583/aad72b
Dojčilović R, Pajović JD, Božanić DK, Jović NG, Pavlović VP, Pavlović VB, Lenhardt Acković L, Zeković IL, Dramićanin M, Kaščakova S, Refregiers M, Rašić G, Vlahović B, Đoković V. DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials. 2018;5(4):045019.
doi:10.1088/2053-1583/aad72b .
Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Jović, Nataša G., Pavlović, Vera P., Pavlović, Vladimir B., Lenhardt Acković, Lea, Zeković, Ivana Lj., Dramićanin, Miroslav, Kaščakova, Slavka, Refregiers, Matthieu, Rašić, Goran, Vlahović, Branislav, Đoković, Vladimir, "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells" in 2D Materials, 5, no. 4 (2018):045019,
https://doi.org/10.1088/2053-1583/aad72b . .
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1

Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study

Dojčilović, Radovan; Pajović, Jelena D.; Božanić, Dušan K.; Bogdanović, Una; Vodnik, Vesna; Dimitrijević-Branković, Suzana I.; Miljković, Miona G.; Kascakova, Slavka; Refregiers, Matthieu; Đoković, Vladimir

(2017)

TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Bogdanović, Una
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana I.
AU  - Miljković, Miona G.
AU  - Kascakova, Slavka
AU  - Refregiers, Matthieu
AU  - Đoković, Vladimir
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1608
AB  - The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells surfaces. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study
VL  - 155
SP  - 341
EP  - 348
DO  - 10.1016/j.colsurfb.2017.04.044
ER  - 
@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Bogdanović, Una and Vodnik, Vesna and Dimitrijević-Branković, Suzana I. and Miljković, Miona G. and Kascakova, Slavka and Refregiers, Matthieu and Đoković, Vladimir",
year = "2017",
abstract = "The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells surfaces. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study",
volume = "155",
pages = "341-348",
doi = "10.1016/j.colsurfb.2017.04.044"
}
Dojčilović, R., Pajović, J. D., Božanić, D. K., Bogdanović, U., Vodnik, V., Dimitrijević-Branković, S. I., Miljković, M. G., Kascakova, S., Refregiers, M.,& Đoković, V.. (2017). Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces. B: Biointerfaces, 155, 341-348.
https://doi.org/10.1016/j.colsurfb.2017.04.044
Dojčilović R, Pajović JD, Božanić DK, Bogdanović U, Vodnik V, Dimitrijević-Branković SI, Miljković MG, Kascakova S, Refregiers M, Đoković V. Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces. B: Biointerfaces. 2017;155:341-348.
doi:10.1016/j.colsurfb.2017.04.044 .
Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Bogdanović, Una, Vodnik, Vesna, Dimitrijević-Branković, Suzana I., Miljković, Miona G., Kascakova, Slavka, Refregiers, Matthieu, Đoković, Vladimir, "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study" in Colloids and Surfaces. B: Biointerfaces, 155 (2017):341-348,
https://doi.org/10.1016/j.colsurfb.2017.04.044 . .
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Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids

Pajović, Jelena D.; Dojčilović, Radovan; Božanić, Dušan K.; Vodnik, Vesna; Dimitrijević-Branković, Suzana I.; Kascakova, Slavka; Refregiers, Matthieu; Markelic, Milica; Đoković, Vladimir

(2016)

TY  - JOUR
AU  - Pajović, Jelena D.
AU  - Dojčilović, Radovan
AU  - Božanić, Dušan K.
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana I.
AU  - Kascakova, Slavka
AU  - Refregiers, Matthieu
AU  - Markelic, Milica
AU  - Đoković, Vladimir
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1107
AB  - Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.
T2  - Optical and Quantum Electronics
T1  - Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids
VL  - 48
IS  - 6
DO  - 10.1007/s11082-016-0578-y
ER  - 
@article{
author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana I. and Kascakova, Slavka and Refregiers, Matthieu and Markelic, Milica and Đoković, Vladimir",
year = "2016",
abstract = "Fluorescent nanostructures were prepared by functionalization of gold nanoparticles with riboflavin molecules and used as probes for synchrotron radiation deep ultraviolet (SR-DUV) fluorescence imaging of gluteraldehyde-fixed Candida albicans cells. The nanoparticles were characterized by transmission electron microscopy (TEM) and optical spectroscopy techniques. The TEM analysis showed that the nanostructures were 6 nm in diameter, while the results of the fluorescence spectroscopies confirmed the photoluminescence of the nanoparticles. The SR-DUV imaging showed that it was possible to distinguish the fluorescence of the nanoparticles from the autofluorescence of the cells, as well as that the local maxima of the signal pertaining to the fluorescence of goldriboflavin nanostructures were mostly positioned on the surfaces of the C. albicans cells of spherical morphology.",
journal = "Optical and Quantum Electronics",
title = "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids",
volume = "48",
number = "6",
doi = "10.1007/s11082-016-0578-y"
}
Pajović, J. D., Dojčilović, R., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S. I., Kascakova, S., Refregiers, M., Markelic, M.,& Đoković, V.. (2016). Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics, 48(6).
https://doi.org/10.1007/s11082-016-0578-y
Pajović JD, Dojčilović R, Božanić DK, Vodnik V, Dimitrijević-Branković SI, Kascakova S, Refregiers M, Markelic M, Đoković V. Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids. in Optical and Quantum Electronics. 2016;48(6).
doi:10.1007/s11082-016-0578-y .
Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana I., Kascakova, Slavka, Refregiers, Matthieu, Markelic, Milica, Đoković, Vladimir, "Deep UV fluorescence imaging study of Candida albicans cells treated with gold-riboflavin hydrocolloids" in Optical and Quantum Electronics, 48, no. 6 (2016),
https://doi.org/10.1007/s11082-016-0578-y . .
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A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution

Dojčilović, Radovan; Pajović, Jelena D.; Božanić, Dušan K.; Vodnik, Vesna; Dimitrijević-Branković, Suzana I.; Milosavljević, Aleksandar R.; Kascakova, S.; Refregiers, M.; Đoković, Vladimir

(2016)

TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana I.
AU  - Milosavljević, Aleksandar R.
AU  - Kascakova, S.
AU  - Refregiers, M.
AU  - Đoković, Vladimir
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/971
AB  - The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.
T2  - Analyst
T1  - A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution
VL  - 141
IS  - 6
SP  - 1988
EP  - 1996
DO  - 10.1039/c5an02358k
ER  - 
@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Vodnik, Vesna and Dimitrijević-Branković, Suzana I. and Milosavljević, Aleksandar R. and Kascakova, S. and Refregiers, M. and Đoković, Vladimir",
year = "2016",
abstract = "The investigation of the interaction of silver nanoparticles and live bacteria cells is of particular importance for understanding and controlling their bactericidal properties. In this study, the process of internalization of silver nanoparticles in Escherichia coli cells was followed by means of synchrotron excitation deep ultraviolet (DUV) fluorescence imaging. Antimicrobial nanostructures that can absorb and emit light in the UV region were prepared by functionalization of silver nanoparticles with tryptophan amino acid and used as environmentally sensitive fluorescent probes. The nanostructures were characterized by morphological (TEM) and spectroscopic methods (UV-vis, FTIR, XPS, and photoluminescence). The TEM images and the analyses of the UV-vis spectra suggested that the addition of tryptophan led to the formation of hybrid nanostructures with pronounced eccentricity and larger sizes with respect to that of the initial silver nanoparticles. The DUV imaging showed that it was possible to distinguish the fluorescent signal pertaining to silver-tryptophan nanostructures from the autofluorescence of the bacteria. The spatial resolution of the fluorescence images was 154 nm which was sufficient to perform analyses of the accumulation of the nanostructures within a single bacterium. The DUV imaging results imply that the tryptophan-functionalized silver nanoparticles interact with cell membranes via insertion of the amino acid into the phospholipid bilayer and enter the cells.",
journal = "Analyst",
title = "A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution",
volume = "141",
number = "6",
pages = "1988-1996",
doi = "10.1039/c5an02358k"
}
Dojčilović, R., Pajović, J. D., Božanić, D. K., Vodnik, V., Dimitrijević-Branković, S. I., Milosavljević, A. R., Kascakova, S., Refregiers, M.,& Đoković, V.. (2016). A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution. in Analyst, 141(6), 1988-1996.
https://doi.org/10.1039/c5an02358k
Dojčilović R, Pajović JD, Božanić DK, Vodnik V, Dimitrijević-Branković SI, Milosavljević AR, Kascakova S, Refregiers M, Đoković V. A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution. in Analyst. 2016;141(6):1988-1996.
doi:10.1039/c5an02358k .
Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Vodnik, Vesna, Dimitrijević-Branković, Suzana I., Milosavljević, Aleksandar R., Kascakova, S., Refregiers, M., Đoković, Vladimir, "A fluorescent nanoprobe for single bacterium tracking: functionalization of silver nanoparticles with tryptophan to probe the nanoparticle accumulation with single cell resolution" in Analyst, 141, no. 6 (2016):1988-1996,
https://doi.org/10.1039/c5an02358k . .
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10

Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells

Pajović, Jelena D.; Dojčilović, Radovan; Božanić, Dušan K.; Kascakova, Slavka; Refregiers, Matthieu; Dimitrijević-Branković, Suzana I.; Vodnik, Vesna; Milosavljević, Aleksandar R.; Piscopiello, Emanuela; Luyt, Adriaan S.; Đoković, Vladimir

(Elsevier, 2015)

TY  - JOUR
AU  - Pajović, Jelena D.
AU  - Dojčilović, Radovan
AU  - Božanić, Dušan K.
AU  - Kascakova, Slavka
AU  - Refregiers, Matthieu
AU  - Dimitrijević-Branković, Suzana I.
AU  - Vodnik, Vesna
AU  - Milosavljević, Aleksandar R.
AU  - Piscopiello, Emanuela
AU  - Luyt, Adriaan S.
AU  - Đoković, Vladimir
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/875
AB  - Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved.
PB  - Elsevier
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
VL  - 135
SP  - 742
EP  - 750
DO  - 10.1016/j.colsurfb.2015.08.050
ER  - 
@article{
author = "Pajović, Jelena D. and Dojčilović, Radovan and Božanić, Dušan K. and Kascakova, Slavka and Refregiers, Matthieu and Dimitrijević-Branković, Suzana I. and Vodnik, Vesna and Milosavljević, Aleksandar R. and Piscopiello, Emanuela and Luyt, Adriaan S. and Đoković, Vladimir",
year = "2015",
abstract = "Biocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8 nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that It is possible to detect these hybrid nanostructures within Escherichia coli cells. (c) 2015 Elsevier B.V. All rights reserved.",
publisher = "Elsevier",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells",
volume = "135",
pages = "742-750",
doi = "10.1016/j.colsurfb.2015.08.050"
}
Pajović, J. D., Dojčilović, R., Božanić, D. K., Kascakova, S., Refregiers, M., Dimitrijević-Branković, S. I., Vodnik, V., Milosavljević, A. R., Piscopiello, E., Luyt, A. S.,& Đoković, V.. (2015). Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces. B: Biointerfaces
Elsevier., 135, 742-750.
https://doi.org/10.1016/j.colsurfb.2015.08.050
Pajović JD, Dojčilović R, Božanić DK, Kascakova S, Refregiers M, Dimitrijević-Branković SI, Vodnik V, Milosavljević AR, Piscopiello E, Luyt AS, Đoković V. Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells. in Colloids and Surfaces. B: Biointerfaces. 2015;135:742-750.
doi:10.1016/j.colsurfb.2015.08.050 .
Pajović, Jelena D., Dojčilović, Radovan, Božanić, Dušan K., Kascakova, Slavka, Refregiers, Matthieu, Dimitrijević-Branković, Suzana I., Vodnik, Vesna, Milosavljević, Aleksandar R., Piscopiello, Emanuela, Luyt, Adriaan S., Đoković, Vladimir, "Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells" in Colloids and Surfaces. B: Biointerfaces, 135 (2015):742-750,
https://doi.org/10.1016/j.colsurfb.2015.08.050 . .
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Structural properties of composites of polyvinylidene fluoride and mechanically activated BaTiO3 particles

Pavlović, Vera P.; Pavlović, Vladimir B.; Vlahović, Branislav; Božanić, Dušan K.; Pajović, Jelena D.; Dojčilović, Radovan; Đoković, Vladimir

(2013)

TY  - JOUR
AU  - Pavlović, Vera P.
AU  - Pavlović, Vladimir B.
AU  - Vlahović, Branislav
AU  - Božanić, Dušan K.
AU  - Pajović, Jelena D.
AU  - Dojčilović, Radovan
AU  - Đoković, Vladimir
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7015
AB  - Nanocomposites of electroactive ceramics and ferroelectric polymers exploit favorable features of the matrix polymer and the nanostructured filler to produce new functional materials for pressure and IR sensors. In this study, the influence of mechanical activation of barium titanate (BaTiO3) particles on the structural properties of BaTiO3/polyvinylidene fluoride (PVDF) nanocomposites was investigated. Nanocomposite films were prepared by the solution casting method and characterized by scanning electron microscopy, x-ray diffraction and Raman spectroscopy. It was found that mechanically activated fillers promote the formation of a ferroelectric beta-phase during crystallization of PVDF.
T2  - Physica Scripta
T1  - Structural properties of composites of polyvinylidene fluoride and mechanically activated BaTiO3 particles
VL  - T157
DO  - 10.1088/0031-8949/2013/T157/014006
ER  - 
@article{
author = "Pavlović, Vera P. and Pavlović, Vladimir B. and Vlahović, Branislav and Božanić, Dušan K. and Pajović, Jelena D. and Dojčilović, Radovan and Đoković, Vladimir",
year = "2013",
abstract = "Nanocomposites of electroactive ceramics and ferroelectric polymers exploit favorable features of the matrix polymer and the nanostructured filler to produce new functional materials for pressure and IR sensors. In this study, the influence of mechanical activation of barium titanate (BaTiO3) particles on the structural properties of BaTiO3/polyvinylidene fluoride (PVDF) nanocomposites was investigated. Nanocomposite films were prepared by the solution casting method and characterized by scanning electron microscopy, x-ray diffraction and Raman spectroscopy. It was found that mechanically activated fillers promote the formation of a ferroelectric beta-phase during crystallization of PVDF.",
journal = "Physica Scripta",
title = "Structural properties of composites of polyvinylidene fluoride and mechanically activated BaTiO3 particles",
volume = "T157",
doi = "10.1088/0031-8949/2013/T157/014006"
}
Pavlović, V. P., Pavlović, V. B., Vlahović, B., Božanić, D. K., Pajović, J. D., Dojčilović, R.,& Đoković, V.. (2013). Structural properties of composites of polyvinylidene fluoride and mechanically activated BaTiO3 particles. in Physica Scripta, T157.
https://doi.org/10.1088/0031-8949/2013/T157/014006
Pavlović VP, Pavlović VB, Vlahović B, Božanić DK, Pajović JD, Dojčilović R, Đoković V. Structural properties of composites of polyvinylidene fluoride and mechanically activated BaTiO3 particles. in Physica Scripta. 2013;T157.
doi:10.1088/0031-8949/2013/T157/014006 .
Pavlović, Vera P., Pavlović, Vladimir B., Vlahović, Branislav, Božanić, Dušan K., Pajović, Jelena D., Dojčilović, Radovan, Đoković, Vladimir, "Structural properties of composites of polyvinylidene fluoride and mechanically activated BaTiO3 particles" in Physica Scripta, T157 (2013),
https://doi.org/10.1088/0031-8949/2013/T157/014006 . .
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