TY  - JOUR
AU  - Knežević, Dragan
AU  - Bošnjaković, Dušan
AU  - Dražić, Slavica
AU  - Nedić, Sreten
AU  - Vujanac, Ivan
AU  - Valčić, Olivera
AU  - Pantelić, Marija
AU  - Stojiljković, Mojca
AU  - Sladojević, Željko
AU  - Kirovski, Danijela
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13027
AB  - The study aimed to determine the concentration of energy-related hormones in cow’s milk and to consider them from a public health perspective. Fourteen Holstein cows were subjected to milk and blood sampling on the following days in lactation (DIL): 10, 30, 60, 90, 150, 180, 210, 250 and 280 to determine milk hormones, fat and protein content and blood biochemical parameters. For the same purpose, bulk-tank milk was sampled and samples of retail m ilk with 1.5% (CM1.5) and 3.2% (CM3.2) fat was purchased. Milk insulin-like growth factor-1 (IGF-1) values were signifi cantly lower at 90, 150, 180, 210 and 250 and signifi cantly higher at 10, 30 and 60 DIL than lactation average (LA). Milk insulin concentrations were signifi cantly lower at 30, 60 and 90 and higher at 210, 250 and 280 DIL than LA. Free thyroxine (fT4) level in the milk was higher at 250 DIL, while milk free triiodothyronine (fT3) concentrations were lower at 30, 60, 90 and 280 DIL, and signifi cantly higher at 10 and 180 DIL than respective LA. Milk cortisol levels were lower at 60 and 280 DIL than LA. All measured milk hormones were signifi cantly lower in CM1.5 compared to CM3.2, bulk-tank milk and LA. An exception was the LA of IGF-1, which was signifi cantly lower than the IGF1 content in CM1.5. Blood biochemical parameters fl uctuated evenly during lactation and were within the reference range. Hormone concentrations in cow’s milk fl uctuate during lactation, giving milk an important role in the context of public health.
AB  - Istraživanje je imalo za cilj određivanje koncentracije hormona u mleku krava u različitim fazama laktacije, mleku iz laktofriza i maloprodajnom mleku. Uzorci mleka i krvi prikupljeni su 10., 30., 60., 90., 150., 180., 210., 250. i 280 dana laktacije od četrnaest krava holštajn rase radi određivanja koncentracije hormona, masti i proteina u mleku i biohemijskih parametara krvi. U iste svrhe uzorkovano je mleko iz laktofriza i nabavljeno je komercijalno mleko sa 1,5% (CM1,5) i 3,2% (CM3,2) mlečne masti. Koncentracije insulinu-sličnog faktora rasta-1 (IGF-1) u mleku bile su značajno niže 90., 150., 180., 210. i 250. dana i značajno više 10., 30. i 60. dana laktacije poredeći sa laktacionim prosekom (LP). Insulin je imao značajno niže koncentracije u mleku 30., 60. i 90. dana i značajno više koncentracije 210., 250. i 280. dana laktacije poredeći sa LP. Koncentracija slobodnog tiroksina (fT4) je bila viša 250. dana, dok je nivo slobodnog trijodotironina (fT3) bio značajno niži 30., 60., 90. i 280. dana i značajno viši 10. i 180. dana u poređenju sa odgovarajućim LP. Nivo kortizola u mleku je bio niži 60. i 280. dana laktacije poredeći sa LP. Svi hormoni su imali značajno niže koncentracije u CM1,5 nego u CM3,2, mleku iz laktofriza i LP. Izuzetak je LP za IGF-1, koji je bio značajno niži nego nivo IGF-1 u CM1,5. Biohemijski parametri krvi su blago fl uktuirali tokom laktacije, ali su ostali u referentnom opsegu. Koncentracije hormona u kravljem mleku fl uktuiraju tokom laktacije, što daje značaj mleku u kontekstu javnog zdravlja.
T2  - Acta Veterinaria
T1  - Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers
T1  - Hormoni u sirovom i maloprodajnom mleku krava i potencijalni rizik po zdravlje potrošača
VL  - 74
IS  - 1
SP  - 1
EP  - 16
DO  - 10.2478/acve-2024-0001
ER  - 

@article{
author = "Knežević, Dragan and Bošnjaković, Dušan and Dražić, Slavica and Nedić, Sreten and Vujanac, Ivan and Valčić, Olivera and Pantelić, Marija and Stojiljković, Mojca and Sladojević, Željko and Kirovski, Danijela",
year = "2024",
abstract = "The study aimed to determine the concentration of energy-related hormones in cow’s milk and to consider them from a public health perspective. Fourteen Holstein cows were subjected to milk and blood sampling on the following days in lactation (DIL): 10, 30, 60, 90, 150, 180, 210, 250 and 280 to determine milk hormones, fat and protein content and blood biochemical parameters. For the same purpose, bulk-tank milk was sampled and samples of retail m ilk with 1.5% (CM1.5) and 3.2% (CM3.2) fat was purchased. Milk insulin-like growth factor-1 (IGF-1) values were signifi cantly lower at 90, 150, 180, 210 and 250 and signifi cantly higher at 10, 30 and 60 DIL than lactation average (LA). Milk insulin concentrations were signifi cantly lower at 30, 60 and 90 and higher at 210, 250 and 280 DIL than LA. Free thyroxine (fT4) level in the milk was higher at 250 DIL, while milk free triiodothyronine (fT3) concentrations were lower at 30, 60, 90 and 280 DIL, and signifi cantly higher at 10 and 180 DIL than respective LA. Milk cortisol levels were lower at 60 and 280 DIL than LA. All measured milk hormones were signifi cantly lower in CM1.5 compared to CM3.2, bulk-tank milk and LA. An exception was the LA of IGF-1, which was signifi cantly lower than the IGF1 content in CM1.5. Blood biochemical parameters fl uctuated evenly during lactation and were within the reference range. Hormone concentrations in cow’s milk fl uctuate during lactation, giving milk an important role in the context of public health., Istraživanje je imalo za cilj određivanje koncentracije hormona u mleku krava u različitim fazama laktacije, mleku iz laktofriza i maloprodajnom mleku. Uzorci mleka i krvi prikupljeni su 10., 30., 60., 90., 150., 180., 210., 250. i 280 dana laktacije od četrnaest krava holštajn rase radi određivanja koncentracije hormona, masti i proteina u mleku i biohemijskih parametara krvi. U iste svrhe uzorkovano je mleko iz laktofriza i nabavljeno je komercijalno mleko sa 1,5% (CM1,5) i 3,2% (CM3,2) mlečne masti. Koncentracije insulinu-sličnog faktora rasta-1 (IGF-1) u mleku bile su značajno niže 90., 150., 180., 210. i 250. dana i značajno više 10., 30. i 60. dana laktacije poredeći sa laktacionim prosekom (LP). Insulin je imao značajno niže koncentracije u mleku 30., 60. i 90. dana i značajno više koncentracije 210., 250. i 280. dana laktacije poredeći sa LP. Koncentracija slobodnog tiroksina (fT4) je bila viša 250. dana, dok je nivo slobodnog trijodotironina (fT3) bio značajno niži 30., 60., 90. i 280. dana i značajno viši 10. i 180. dana u poređenju sa odgovarajućim LP. Nivo kortizola u mleku je bio niži 60. i 280. dana laktacije poredeći sa LP. Svi hormoni su imali značajno niže koncentracije u CM1,5 nego u CM3,2, mleku iz laktofriza i LP. Izuzetak je LP za IGF-1, koji je bio značajno niži nego nivo IGF-1 u CM1,5. Biohemijski parametri krvi su blago fl uktuirali tokom laktacije, ali su ostali u referentnom opsegu. Koncentracije hormona u kravljem mleku fl uktuiraju tokom laktacije, što daje značaj mleku u kontekstu javnog zdravlja.",
journal = "Acta Veterinaria",
title = "Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers, Hormoni u sirovom i maloprodajnom mleku krava i potencijalni rizik po zdravlje potrošača",
volume = "74",
number = "1",
pages = "1-16",
doi = "10.2478/acve-2024-0001"
}

Knežević, D., Bošnjaković, D., Dražić, S., Nedić, S., Vujanac, I., Valčić, O., Pantelić, M., Stojiljković, M., Sladojević, Ž.,& Kirovski, D.. (2024). Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers. in Acta Veterinaria, 74(1), 1-16.
https://doi.org/10.2478/acve-2024-0001

Knežević D, Bošnjaković D, Dražić S, Nedić S, Vujanac I, Valčić O, Pantelić M, Stojiljković M, Sladojević Ž, Kirovski D. Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers. in Acta Veterinaria. 2024;74(1):1-16.
doi:10.2478/acve-2024-0001 .

Knežević, Dragan, Bošnjaković, Dušan, Dražić, Slavica, Nedić, Sreten, Vujanac, Ivan, Valčić, Olivera, Pantelić, Marija, Stojiljković, Mojca, Sladojević, Željko, Kirovski, Danijela, "Energy-Related Hormones in Raw and Retail Cow’s Milk and Possible Risk for Consumers" in Acta Veterinaria, 74, no. 1 (2024):1-16,
https://doi.org/10.2478/acve-2024-0001 . .

TY  - JOUR
AU  - Nedić, Sreten
AU  - Pantelić, Marija
AU  - Vranješ-Đurić, Sanja
AU  - Nedić, Drago
AU  - Jovanović, Ljubomir J.
AU  - Cebulj-Kadunc, Nina
AU  - Kobal, Silvestra
AU  - Snoj, Tomaž
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1894
AB  - Cortisol levels were measured in hair, blood and milk in two different cattle breeds, kept under different breeding conditions and with different genetic merit for milk production. Cows and heifers of Holstein and Busha breeds were selected for the study. Cortisol concentration was determined by immunoassays. Cortisol accumulation was determined in proximal (close to the skin) and distal (far from the skin) segments of the hair shaft. The influence of hair colour and washing prior to extraction and analysis was also examined in order to establish additional factors that may have an impact on hair cortisol concentrations. Concentrations of cortisol determined in the proximal and distal segments of the shaft were significantly higher in Holstein than Busha cows and heifers (P LT 0.05 and P LT 0.01, respectively). In Holstein cows, no significant difference was found between concentrations in black and white hair. In hair washed with isopropanol, cortisol concentration was significantly lower compared to unwashed hair (P LT 0.01). Thus, cortisol concentration in hair varies with the technique of hair processing (washing), but not with colour in Holstein cows. Blood serum cortisol concentrations in Holstein cows and heifers were significantly higher than in Busha cows and heifers, (P LT 0.01 and P LT 0.05, respectively). Milk cortisol in Holstein cows was significantly higher than in Busha cows (P LT 0.05). The higher cortisol concentrations in Holstein cows are assumed to be the result of intensive breeding and physiological adaptation to high milk production.
T2  - Slovenian Veterinary Research
T1  - Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle
VL  - 54
IS  - 4
SP  - 163
EP  - 172
DO  - 10.26873/SVR-398-2017
ER  - 

@article{
author = "Nedić, Sreten and Pantelić, Marija and Vranješ-Đurić, Sanja and Nedić, Drago and Jovanović, Ljubomir J. and Cebulj-Kadunc, Nina and Kobal, Silvestra and Snoj, Tomaž and Kirovski, Danijela",
year = "2017",
abstract = "Cortisol levels were measured in hair, blood and milk in two different cattle breeds, kept under different breeding conditions and with different genetic merit for milk production. Cows and heifers of Holstein and Busha breeds were selected for the study. Cortisol concentration was determined by immunoassays. Cortisol accumulation was determined in proximal (close to the skin) and distal (far from the skin) segments of the hair shaft. The influence of hair colour and washing prior to extraction and analysis was also examined in order to establish additional factors that may have an impact on hair cortisol concentrations. Concentrations of cortisol determined in the proximal and distal segments of the shaft were significantly higher in Holstein than Busha cows and heifers (P LT 0.05 and P LT 0.01, respectively). In Holstein cows, no significant difference was found between concentrations in black and white hair. In hair washed with isopropanol, cortisol concentration was significantly lower compared to unwashed hair (P LT 0.01). Thus, cortisol concentration in hair varies with the technique of hair processing (washing), but not with colour in Holstein cows. Blood serum cortisol concentrations in Holstein cows and heifers were significantly higher than in Busha cows and heifers, (P LT 0.01 and P LT 0.05, respectively). Milk cortisol in Holstein cows was significantly higher than in Busha cows (P LT 0.05). The higher cortisol concentrations in Holstein cows are assumed to be the result of intensive breeding and physiological adaptation to high milk production.",
journal = "Slovenian Veterinary Research",
title = "Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle",
volume = "54",
number = "4",
pages = "163-172",
doi = "10.26873/SVR-398-2017"
}

Nedić, S., Pantelić, M., Vranješ-Đurić, S., Nedić, D., Jovanović, L. J., Cebulj-Kadunc, N., Kobal, S., Snoj, T.,& Kirovski, D.. (2017). Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle. in Slovenian Veterinary Research, 54(4), 163-172.
https://doi.org/10.26873/SVR-398-2017

Nedić S, Pantelić M, Vranješ-Đurić S, Nedić D, Jovanović LJ, Cebulj-Kadunc N, Kobal S, Snoj T, Kirovski D. Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle. in Slovenian Veterinary Research. 2017;54(4):163-172.
doi:10.26873/SVR-398-2017 .

Nedić, Sreten, Pantelić, Marija, Vranješ-Đurić, Sanja, Nedić, Drago, Jovanović, Ljubomir J., Cebulj-Kadunc, Nina, Kobal, Silvestra, Snoj, Tomaž, Kirovski, Danijela, "Cortisol Concentrations in Hair, Blood and Milk of Holstein and Busha Cattle" in Slovenian Veterinary Research, 54, no. 4 (2017):163-172,
https://doi.org/10.26873/SVR-398-2017 . .

TY  - JOUR
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Bojkovski, Jovan
AU  - Simeunović, Predrag
AU  - Palamarevic, Milija
AU  - Nedić, Sreten
AU  - Celeska, Irena
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1366
AB  - This study aimed to evaluate glucose-induced insulin response in cows exposed to different temperature-humidity index. Twenty early lactating Holstein-Friesian cows were divided into 2 equal groups based on season, as summer (SU) and spring (SP). SP cows were not exposed to heat stress, while SU cows were exposed to moderate or severe heat stress. Milk production was recorded daily. Starting from day 30 of lactation, intravenous glucose tolerance test (IVGTT) was carried out three times at 30-day intervals. Blood samples were taken before (basal) and after glucose infusion, and glucose and insulin were measured at each sample point. The homeostatic model assessment (HOMA) index was calculated. Milk yield from days 30 to 40 and 64 to 90 of lactation were higher in SP cows than in SU cows. Basal glucose did not differ on days 30 and 60 of lactation, while basal insulin and HOMA were lower in SU compared to SP cows. On day 90 of lactation, SU cows had higher basal glucose, whereas basal insulin and HOMA did not differ. IVGTT results revealed that glucose tolerance was affected by heat stress such that SU cows had higher glucose clearance. Insulin responses to IVGTT did not differ on days 30 and 60 of lactation. Heat stress had a marked effect on insulin secretion on day 90 of lactation, illustrated by higher increments, peak concentrations and area under the curve for insulin in SU cows. Overall, season differences in glucose tolerance depend not only on heat stress and milk production but also on the stage of lactation.
T2  - Acta Veterinaria, Beograd
T1  - Field Trial on Glucose-Induced Insulin Response in High-Yielding Dairy Cows under Different Environmental Temperatures
VL  - 67
IS  - 3
SP  - 366
EP  - 382
DO  - 10.1515/acve-2017-0030
ER  - 

@article{
author = "Vujanac, Ivan and Prodanović, Radiša and Korićanac, Goran and Bojkovski, Jovan and Simeunović, Predrag and Palamarevic, Milija and Nedić, Sreten and Celeska, Irena and Kirovski, Danijela",
year = "2017",
abstract = "This study aimed to evaluate glucose-induced insulin response in cows exposed to different temperature-humidity index. Twenty early lactating Holstein-Friesian cows were divided into 2 equal groups based on season, as summer (SU) and spring (SP). SP cows were not exposed to heat stress, while SU cows were exposed to moderate or severe heat stress. Milk production was recorded daily. Starting from day 30 of lactation, intravenous glucose tolerance test (IVGTT) was carried out three times at 30-day intervals. Blood samples were taken before (basal) and after glucose infusion, and glucose and insulin were measured at each sample point. The homeostatic model assessment (HOMA) index was calculated. Milk yield from days 30 to 40 and 64 to 90 of lactation were higher in SP cows than in SU cows. Basal glucose did not differ on days 30 and 60 of lactation, while basal insulin and HOMA were lower in SU compared to SP cows. On day 90 of lactation, SU cows had higher basal glucose, whereas basal insulin and HOMA did not differ. IVGTT results revealed that glucose tolerance was affected by heat stress such that SU cows had higher glucose clearance. Insulin responses to IVGTT did not differ on days 30 and 60 of lactation. Heat stress had a marked effect on insulin secretion on day 90 of lactation, illustrated by higher increments, peak concentrations and area under the curve for insulin in SU cows. Overall, season differences in glucose tolerance depend not only on heat stress and milk production but also on the stage of lactation.",
journal = "Acta Veterinaria, Beograd",
title = "Field Trial on Glucose-Induced Insulin Response in High-Yielding Dairy Cows under Different Environmental Temperatures",
volume = "67",
number = "3",
pages = "366-382",
doi = "10.1515/acve-2017-0030"
}

Vujanac, I., Prodanović, R., Korićanac, G., Bojkovski, J., Simeunović, P., Palamarevic, M., Nedić, S., Celeska, I.,& Kirovski, D.. (2017). Field Trial on Glucose-Induced Insulin Response in High-Yielding Dairy Cows under Different Environmental Temperatures. in Acta Veterinaria, Beograd, 67(3), 366-382.
https://doi.org/10.1515/acve-2017-0030

Vujanac I, Prodanović R, Korićanac G, Bojkovski J, Simeunović P, Palamarevic M, Nedić S, Celeska I, Kirovski D. Field Trial on Glucose-Induced Insulin Response in High-Yielding Dairy Cows under Different Environmental Temperatures. in Acta Veterinaria, Beograd. 2017;67(3):366-382.
doi:10.1515/acve-2017-0030 .

Vujanac, Ivan, Prodanović, Radiša, Korićanac, Goran, Bojkovski, Jovan, Simeunović, Predrag, Palamarevic, Milija, Nedić, Sreten, Celeska, Irena, Kirovski, Danijela, "Field Trial on Glucose-Induced Insulin Response in High-Yielding Dairy Cows under Different Environmental Temperatures" in Acta Veterinaria, Beograd, 67, no. 3 (2017):366-382,
https://doi.org/10.1515/acve-2017-0030 . .