TY  - JOUR
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Kolaković, Ana
AU  - Đurđević, Vladimir
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5869
AB  - Glutathione S-transferases (GSTs) carry out a wide range of functions in cells, such as detoxification of endogenous compounds, removal of reactive oxygen species, and even catalysis of reactions in metabolic pathways beyond detoxification. Based on previous research, GSTM1 and GSTT1 might modify the risk of atherosclerosis. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the occurrence of carotid plaque (CP); and biochemical parameters of oxidative stress, lipid profile and inflammation, in 346 consecutive patients with advanced atherosclerosis that underwent endarterectomy. A multiplex polymerase chain reaction (PCR) method was used to detect the deletions in GSTM1 and GSTT1 genes in the genomic DNA in 346 patients and 330 controls. The adjusted OR for CP presence (adjusted for age, gender, smoking, hypertension, BMI, HDLC, TG) was 0.24, 95 %CI 0.08-0.7, p LT 0.01 for GSTT1 null and 1.13, 95 %CI 0.62-2.07, p = 0.7 for GSTM1 null genotype. We found significantly lower plasma lipoprotein (a) (Lp(a)) levels in GSTT1 null compared to wild-type genotype carriers in patient group (20.68 +/- A 26.02 mg/dl vs. 40.66 +/- A 42.89 mg/dl, mean +/- A SD, p = 0.04). The serum interleukin-6 (IL-6) values were significantly influenced by both GST polymorphisms in patients with CP. Our results, showing the significant reduction of GSTT1 deletions in patients with CP, suggest involvement of GSTs in carotid atherosclerosis. This study shows additional view of the possible role of GSTs in advanced chronic inflammatory disease of vascular system, but the confirmation in a larger studies in different populations are needed.
T2  - Molecular Biology Reports
T1  - Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters
VL  - 41
IS  - 2
SP  - 1157
EP  - 1164
DO  - 10.1007/s11033-013-2962-z
ER  - 

@article{
author = "Živković, Maja and Stanković, Aleksandra and Đurić, Tamara and Končar, Igor and Kolaković, Ana and Đurđević, Vladimir and Davidović, Lazar and Alavantić, Dragan",
year = "2014",
abstract = "Glutathione S-transferases (GSTs) carry out a wide range of functions in cells, such as detoxification of endogenous compounds, removal of reactive oxygen species, and even catalysis of reactions in metabolic pathways beyond detoxification. Based on previous research, GSTM1 and GSTT1 might modify the risk of atherosclerosis. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the occurrence of carotid plaque (CP); and biochemical parameters of oxidative stress, lipid profile and inflammation, in 346 consecutive patients with advanced atherosclerosis that underwent endarterectomy. A multiplex polymerase chain reaction (PCR) method was used to detect the deletions in GSTM1 and GSTT1 genes in the genomic DNA in 346 patients and 330 controls. The adjusted OR for CP presence (adjusted for age, gender, smoking, hypertension, BMI, HDLC, TG) was 0.24, 95 %CI 0.08-0.7, p LT 0.01 for GSTT1 null and 1.13, 95 %CI 0.62-2.07, p = 0.7 for GSTM1 null genotype. We found significantly lower plasma lipoprotein (a) (Lp(a)) levels in GSTT1 null compared to wild-type genotype carriers in patient group (20.68 +/- A 26.02 mg/dl vs. 40.66 +/- A 42.89 mg/dl, mean +/- A SD, p = 0.04). The serum interleukin-6 (IL-6) values were significantly influenced by both GST polymorphisms in patients with CP. Our results, showing the significant reduction of GSTT1 deletions in patients with CP, suggest involvement of GSTs in carotid atherosclerosis. This study shows additional view of the possible role of GSTs in advanced chronic inflammatory disease of vascular system, but the confirmation in a larger studies in different populations are needed.",
journal = "Molecular Biology Reports",
title = "Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters",
volume = "41",
number = "2",
pages = "1157-1164",
doi = "10.1007/s11033-013-2962-z"
}

Živković, M., Stanković, A., Đurić, T., Končar, I., Kolaković, A., Đurđević, V., Davidović, L.,& Alavantić, D.. (2014). Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters. in Molecular Biology Reports, 41(2), 1157-1164.
https://doi.org/10.1007/s11033-013-2962-z

Živković M, Stanković A, Đurić T, Končar I, Kolaković A, Đurđević V, Davidović L, Alavantić D. Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters. in Molecular Biology Reports. 2014;41(2):1157-1164.
doi:10.1007/s11033-013-2962-z .

Živković, Maja, Stanković, Aleksandra, Đurić, Tamara, Končar, Igor, Kolaković, Ana, Đurđević, Vladimir, Davidović, Lazar, Alavantić, Dragan, "Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters" in Molecular Biology Reports, 41, no. 2 (2014):1157-1164,
https://doi.org/10.1007/s11033-013-2962-z . .