TY  - JOUR
AU  - Kolaković, Ana
AU  - Bundalo, Maja
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13103
AB  - Background/Aim. The renin-angiotensin system (RAS) is linked to the development of atherosclerosis (As), including its initiation and progression. Besides the well-known angio-tensin-converting enzyme (ACE), two newer RAS family members are related to vascular remodeling - ACE2 as a homolog of ACE and collectrin [transmembrane protein 27 (TMEM27)] as a homolog of ACE2. Up to now, a limited number of studies have examined the expression of these RAS components in advanced carotid plaque (CP) tissue based on the sex of the patients and plaque phenotypes (PPs). There are two ultrasonographically defined PPs - the hypoechogenic plaque (HoP) and the hyperechogenic plaque (HerP) phenotype. The aim of the study was to investigate whether there was a correlation between the expression of RAS components in the CP and the sex and PPs of patients. Methods. We examined 74 patients with advanced CP who underwent carotid endarterectomy. The intraplaque expression of RAS components was determined with the real-time polymerase chain reaction, using the TaqMan® gene expression assays and Western blot. A two-way ANOVA followed by a post-hoc Tukey test was performed for the statistical analysis of results. Results. No interaction was recorded between the sex of the patients and PPs in influencing the relative expression of ACE and TMEM27 messenger RNA (mRNA) (p > 0.05). In 56.06% of plaque samples, no expression of ACE2 mRNA was detected. Among the plaques where ACE2 mRNA expression was detected, its expression level was higher in females with the HoP phenotype compared to females with the HerP phenotype (p < 0.001). In patients with the HoP phenotype, females had higher expression of ACE2 mRNA than males (p < 0.05). In the male study group, ACE protein levels were significantly lower in the HoP phenotype compared to the HerP phenotype (p < 0.001). Fe-males with the HoP phenotype had significantly higher ACE protein levels than males with the HoP phenotype (p < 0.0001). Conclusion. Our results revealed alterations in the expression levels of ACE and ACE2, at the mRNA and protein levels, in advanced carotid As. These alterations are impacted by sex and PP and may indicate a switch from the balanced RAS/ACE/ACE2 axis in the healthy blood vessel to the unbalanced axis in vascular remodeling due to As.
AB  - Renin-angiotenzin sistem (RAS) povezan je sa
razvojem ateroskleroze (As), uključujući njen nastanak i
progresiju. Pored dobro poznatog angiotenzinkonvertujućeg enzima (angiotensin-converting enzyme – ACE),
dva nova člana RAS familije povezana su sa
remodelovanjem zidova krvnih sudova – ACE2 kao
homolog ACE i kolektrin [transmembrane protein 27
(TMEM27)] kao homolog ACE2. Do sada je mali broj
studija ispitivao ekspresiju komponenti RAS sistema u tkivu
uznapredovalog karotidnog plaka (KP) u odnosu na pol
bolesnika i fenotip plaka (FP). Postoje dva tipa KP
definisana primenom ultrazvuka – fenotip hipoehogenog
plaka (HoP) i fenotip hiperehogenog plaka (HerP). Cilj rada
bio je da se ispita da li postoji korelacija između ekspresije
komponenti RAS u KP i pola i FP bolesnika. Metode.
Ispitano je 74 bolesnika sa uznapredovalim KP koji su bili
podvrgnuti operativnoj proceduri karotidne
endarterektomije. Ekspresija komponenti RAS sistema u
tkivu plaka utvrđena je lančanom reakcijom polimeraze u
realnom vremenu (real-time polymerase chain reaction)primenom eseja TaqMan® tehnologije i metode Western blota. Dvosmerna analiza varijanse i Tukey post-hoc test korišćen su za statističku obradu rezultata. Rezultati. Nije utvrđena
interakcija FP i pola bolesnika u uticaju na relativnu
ekspresiju informacione RNK (iRNK) za ACE i TMEM27
(p > 0,05). U 56,06% uzoraka plaka nije detektovana
ekspresija iRNK za ACE2. U plakovima u kojima je
detektovana ekspresija iRNK za ACE2, njen nivo bio je viši
kod žena sa HoP u poređenju sa ženama sa HerP (p <
0,001). U grupi bolesnika sa fenotipom HoP, žene su imale
viši nivo iRNK za ACE2 nego muškarci (p < 0,05). U grupi
muškaraca, nivoi ekspresije ACE proteina bili suznačajno
niži u fenotipu HoP u poređenju sa HerP (p < 0,001). Žene
sa fenotipom HoP imale su značajno viši nivo ACE
proteina u poređenju sa muškarcima sa HoP (p < 0,0001).
Zaključak. Naši rezultati pokazali su da postoje promene
nivoa ekspresije ACE i ACE2, na nivou proteina i iRNK u
uznapredovaloj karotidnoj As. Te promene zavise od pola i
FP i mogu ukazivati na to da balans ose RAS/ACE/ACE2
koji postoji u zdravom krvnom sudu postaje poremećen
tokom remodelovanja zida krvnog suda usled As.
T2  - Vojnosanitetski pregled
T1  - The expression of renin-angiotensin system components in human carotid plaque
T1  - Ekspresija komponenti renin-angiotenzin sistema u humanom karotidnom plaku
DO  - 10.2298/VSP221028014K
ER  - 

@article{
author = "Kolaković, Ana and Bundalo, Maja and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra and Živković, Maja",
year = "2024",
abstract = "Background/Aim. The renin-angiotensin system (RAS) is linked to the development of atherosclerosis (As), including its initiation and progression. Besides the well-known angio-tensin-converting enzyme (ACE), two newer RAS family members are related to vascular remodeling - ACE2 as a homolog of ACE and collectrin [transmembrane protein 27 (TMEM27)] as a homolog of ACE2. Up to now, a limited number of studies have examined the expression of these RAS components in advanced carotid plaque (CP) tissue based on the sex of the patients and plaque phenotypes (PPs). There are two ultrasonographically defined PPs - the hypoechogenic plaque (HoP) and the hyperechogenic plaque (HerP) phenotype. The aim of the study was to investigate whether there was a correlation between the expression of RAS components in the CP and the sex and PPs of patients. Methods. We examined 74 patients with advanced CP who underwent carotid endarterectomy. The intraplaque expression of RAS components was determined with the real-time polymerase chain reaction, using the TaqMan® gene expression assays and Western blot. A two-way ANOVA followed by a post-hoc Tukey test was performed for the statistical analysis of results. Results. No interaction was recorded between the sex of the patients and PPs in influencing the relative expression of ACE and TMEM27 messenger RNA (mRNA) (p > 0.05). In 56.06% of plaque samples, no expression of ACE2 mRNA was detected. Among the plaques where ACE2 mRNA expression was detected, its expression level was higher in females with the HoP phenotype compared to females with the HerP phenotype (p < 0.001). In patients with the HoP phenotype, females had higher expression of ACE2 mRNA than males (p < 0.05). In the male study group, ACE protein levels were significantly lower in the HoP phenotype compared to the HerP phenotype (p < 0.001). Fe-males with the HoP phenotype had significantly higher ACE protein levels than males with the HoP phenotype (p < 0.0001). Conclusion. Our results revealed alterations in the expression levels of ACE and ACE2, at the mRNA and protein levels, in advanced carotid As. These alterations are impacted by sex and PP and may indicate a switch from the balanced RAS/ACE/ACE2 axis in the healthy blood vessel to the unbalanced axis in vascular remodeling due to As., Renin-angiotenzin sistem (RAS) povezan je sa
razvojem ateroskleroze (As), uključujući njen nastanak i
progresiju. Pored dobro poznatog angiotenzinkonvertujućeg enzima (angiotensin-converting enzyme – ACE),
dva nova člana RAS familije povezana su sa
remodelovanjem zidova krvnih sudova – ACE2 kao
homolog ACE i kolektrin [transmembrane protein 27
(TMEM27)] kao homolog ACE2. Do sada je mali broj
studija ispitivao ekspresiju komponenti RAS sistema u tkivu
uznapredovalog karotidnog plaka (KP) u odnosu na pol
bolesnika i fenotip plaka (FP). Postoje dva tipa KP
definisana primenom ultrazvuka – fenotip hipoehogenog
plaka (HoP) i fenotip hiperehogenog plaka (HerP). Cilj rada
bio je da se ispita da li postoji korelacija između ekspresije
komponenti RAS u KP i pola i FP bolesnika. Metode.
Ispitano je 74 bolesnika sa uznapredovalim KP koji su bili
podvrgnuti operativnoj proceduri karotidne
endarterektomije. Ekspresija komponenti RAS sistema u
tkivu plaka utvrđena je lančanom reakcijom polimeraze u
realnom vremenu (real-time polymerase chain reaction)primenom eseja TaqMan® tehnologije i metode Western blota. Dvosmerna analiza varijanse i Tukey post-hoc test korišćen su za statističku obradu rezultata. Rezultati. Nije utvrđena
interakcija FP i pola bolesnika u uticaju na relativnu
ekspresiju informacione RNK (iRNK) za ACE i TMEM27
(p > 0,05). U 56,06% uzoraka plaka nije detektovana
ekspresija iRNK za ACE2. U plakovima u kojima je
detektovana ekspresija iRNK za ACE2, njen nivo bio je viši
kod žena sa HoP u poređenju sa ženama sa HerP (p <
0,001). U grupi bolesnika sa fenotipom HoP, žene su imale
viši nivo iRNK za ACE2 nego muškarci (p < 0,05). U grupi
muškaraca, nivoi ekspresije ACE proteina bili suznačajno
niži u fenotipu HoP u poređenju sa HerP (p < 0,001). Žene
sa fenotipom HoP imale su značajno viši nivo ACE
proteina u poređenju sa muškarcima sa HoP (p < 0,0001).
Zaključak. Naši rezultati pokazali su da postoje promene
nivoa ekspresije ACE i ACE2, na nivou proteina i iRNK u
uznapredovaloj karotidnoj As. Te promene zavise od pola i
FP i mogu ukazivati na to da balans ose RAS/ACE/ACE2
koji postoji u zdravom krvnom sudu postaje poremećen
tokom remodelovanja zida krvnog suda usled As.",
journal = "Vojnosanitetski pregled",
title = "The expression of renin-angiotensin system components in human carotid plaque, Ekspresija komponenti renin-angiotenzin sistema u humanom karotidnom plaku",
doi = "10.2298/VSP221028014K"
}

Kolaković, A., Bundalo, M., Đurić, T., Končar, I., Stanković, A.,& Živković, M.. (2024). The expression of renin-angiotensin system components in human carotid plaque. in Vojnosanitetski pregled.
https://doi.org/10.2298/VSP221028014K

Kolaković A, Bundalo M, Đurić T, Končar I, Stanković A, Živković M. The expression of renin-angiotensin system components in human carotid plaque. in Vojnosanitetski pregled. 2024;.
doi:10.2298/VSP221028014K .

Kolaković, Ana, Bundalo, Maja, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, Živković, Maja, "The expression of renin-angiotensin system components in human carotid plaque" in Vojnosanitetski pregled (2024),
https://doi.org/10.2298/VSP221028014K . .

TY  - JOUR
AU  - Stojković, Ljiljana
AU  - Zec, Manja
AU  - Živković, Maja
AU  - Bundalo, Maja
AU  - Bošković, Maja
AU  - Glibetić, Marija
AU  - Stanković, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9865
AB  - Cardiovascular disease (CVD) is associated with alterations in DNA methylation and polyunsaturated fatty acid (PUFA) profile, both modulated by dietary polyphenols. The present parallel, placebo-controlled study (part of the original clinical study registered as NCT02800967 at www.clinicaltrials.gov) aimed to determine the impact of 4-week daily consumption of polyphenol-rich Aronia melanocarpa juice (AMJ) treatment on Long Interspersed Nucleotide Element-1 (LINE-1) methylation in peripheral blood leukocytes and on plasma PUFAs, in subjects (n = 54, age range of 40.2 ± 6.7 years) at moderate CVD risk, including an increased body mass index, central obesity, high normal blood pressure, and/or dyslipidemia. The goal was also to examine whether factors known to affect DNA methylation (folate intake levels, MTHFR C677T gene variant, anthropometric and metabolic parameters) modulated the LINE-1 methylation levels upon the consumption of polyphenol-rich aronia juice. Experimental analysis of LINE-1 methylation was done by MethyLight method. MTHFR C677T genotypes were determined by the polymerase chain reaction–restriction fragment length polymorphism method, and folate intake was assessed by processing the data from the food frequency questionnaire. PUFAs were measured by gas–liquid chromatography, and serum lipid profile was determined by using Roche Diagnostics kits. The statistical analyses were performed using Statistica software package. In the comparison after vs. before the treatment period, in dyslipidemic women (n = 22), we observed significant decreases in LINE-1 methylation levels (97.54 ± 1.50 vs. 98.39 ± 0.86%, respectively; P = 0.01) and arachidonic acid/eicosapentaenoic acid ratio [29.17 ± 15.21 vs. 38.42 (25.96–89.58), respectively; P = 0.02]. The change (after vs. before treatment) in LINE-1 methylation directly correlated with the presence of MTHFR 677T allele, average daily folate intake, and the change in serum low-density lipoprotein cholesterol but inversely correlated with the change in serum triacylglycerols (R = 0.72, R2 = 0.52, adjusted R2 = 0.36, P = 0.03). The current results imply potential cardioprotective effects of habitual polyphenol-rich aronia juice consumption achieved through the modifications of DNA methylation pattern and PUFAs in subjects at CVD risk, which should be further confirmed. Hence, the precision nutrition-driven modulations of both DNA methylation and PUFA profile may become targets for new approaches in the prevention of CVD.
T2  - Frontiers in Nutrition
T1  - Polyphenol-Rich Aronia melanocarpa Juice Consumption Affects LINE-1 DNA Methylation in Peripheral Blood Leukocytes in Dyslipidemic Women
VL  - 8
DO  - 10.3389/fnut.2021.689055
ER  - 

@article{
author = "Stojković, Ljiljana and Zec, Manja and Živković, Maja and Bundalo, Maja and Bošković, Maja and Glibetić, Marija and Stanković, Aleksandra",
year = "2021",
abstract = "Cardiovascular disease (CVD) is associated with alterations in DNA methylation and polyunsaturated fatty acid (PUFA) profile, both modulated by dietary polyphenols. The present parallel, placebo-controlled study (part of the original clinical study registered as NCT02800967 at www.clinicaltrials.gov) aimed to determine the impact of 4-week daily consumption of polyphenol-rich Aronia melanocarpa juice (AMJ) treatment on Long Interspersed Nucleotide Element-1 (LINE-1) methylation in peripheral blood leukocytes and on plasma PUFAs, in subjects (n = 54, age range of 40.2 ± 6.7 years) at moderate CVD risk, including an increased body mass index, central obesity, high normal blood pressure, and/or dyslipidemia. The goal was also to examine whether factors known to affect DNA methylation (folate intake levels, MTHFR C677T gene variant, anthropometric and metabolic parameters) modulated the LINE-1 methylation levels upon the consumption of polyphenol-rich aronia juice. Experimental analysis of LINE-1 methylation was done by MethyLight method. MTHFR C677T genotypes were determined by the polymerase chain reaction–restriction fragment length polymorphism method, and folate intake was assessed by processing the data from the food frequency questionnaire. PUFAs were measured by gas–liquid chromatography, and serum lipid profile was determined by using Roche Diagnostics kits. The statistical analyses were performed using Statistica software package. In the comparison after vs. before the treatment period, in dyslipidemic women (n = 22), we observed significant decreases in LINE-1 methylation levels (97.54 ± 1.50 vs. 98.39 ± 0.86%, respectively; P = 0.01) and arachidonic acid/eicosapentaenoic acid ratio [29.17 ± 15.21 vs. 38.42 (25.96–89.58), respectively; P = 0.02]. The change (after vs. before treatment) in LINE-1 methylation directly correlated with the presence of MTHFR 677T allele, average daily folate intake, and the change in serum low-density lipoprotein cholesterol but inversely correlated with the change in serum triacylglycerols (R = 0.72, R2 = 0.52, adjusted R2 = 0.36, P = 0.03). The current results imply potential cardioprotective effects of habitual polyphenol-rich aronia juice consumption achieved through the modifications of DNA methylation pattern and PUFAs in subjects at CVD risk, which should be further confirmed. Hence, the precision nutrition-driven modulations of both DNA methylation and PUFA profile may become targets for new approaches in the prevention of CVD.",
journal = "Frontiers in Nutrition",
title = "Polyphenol-Rich Aronia melanocarpa Juice Consumption Affects LINE-1 DNA Methylation in Peripheral Blood Leukocytes in Dyslipidemic Women",
volume = "8",
doi = "10.3389/fnut.2021.689055"
}

Stojković, L., Zec, M., Živković, M., Bundalo, M., Bošković, M., Glibetić, M.,& Stanković, A.. (2021). Polyphenol-Rich Aronia melanocarpa Juice Consumption Affects LINE-1 DNA Methylation in Peripheral Blood Leukocytes in Dyslipidemic Women. in Frontiers in Nutrition, 8.
https://doi.org/10.3389/fnut.2021.689055

Stojković L, Zec M, Živković M, Bundalo M, Bošković M, Glibetić M, Stanković A. Polyphenol-Rich Aronia melanocarpa Juice Consumption Affects LINE-1 DNA Methylation in Peripheral Blood Leukocytes in Dyslipidemic Women. in Frontiers in Nutrition. 2021;8.
doi:10.3389/fnut.2021.689055 .

Stojković, Ljiljana, Zec, Manja, Živković, Maja, Bundalo, Maja, Bošković, Maja, Glibetić, Marija, Stanković, Aleksandra, "Polyphenol-Rich Aronia melanocarpa Juice Consumption Affects LINE-1 DNA Methylation in Peripheral Blood Leukocytes in Dyslipidemic Women" in Frontiers in Nutrition, 8 (2021),
https://doi.org/10.3389/fnut.2021.689055 . .

TY  - JOUR
AU  - Bošković, Maja
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7984
AB  - Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.
T2  - Journal of Functional Foods
T1  - Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats
VL  - 52
SP  - 690
EP  - 698
DO  - 10.1016/j.jff.2018.11.053
ER  - 

@article{
author = "Bošković, Maja and Bundalo, Maja M. and Živković, Maja and Stanišić, Jelena and Kostić, Milan and Korićanac, Goran and Stanković, Aleksandra",
year = "2019",
abstract = "Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.",
journal = "Journal of Functional Foods",
title = "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats",
volume = "52",
pages = "690-698",
doi = "10.1016/j.jff.2018.11.053"
}

Bošković, M., Bundalo, M. M., Živković, M., Stanišić, J., Kostić, M., Korićanac, G.,& Stanković, A.. (2019). Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats. in Journal of Functional Foods, 52, 690-698.
https://doi.org/10.1016/j.jff.2018.11.053

Bošković M, Bundalo MM, Živković M, Stanišić J, Kostić M, Korićanac G, Stanković A. Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats. in Journal of Functional Foods. 2019;52:690-698.
doi:10.1016/j.jff.2018.11.053 .

Bošković, Maja, Bundalo, Maja M., Živković, Maja, Stanišić, Jelena, Kostić, Milan, Korićanac, Goran, Stanković, Aleksandra, "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats" in Journal of Functional Foods, 52 (2019):690-698,
https://doi.org/10.1016/j.jff.2018.11.053 . .

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Stojiljković, Mojca D.
AU  - Stanković, Aleksandra
AU  - Živković, Maja
AU  - Korićanac, Goran
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1726
AB  - Increased intake of fructose in humans and laboratory animals is demonstrated to be a risk factor for development of metabolic disorders (insulin resistance, metabolic syndrome, type 2 diabetes) and cardiovascular diseases. On the other hand, estradiol is emphasized as a cardioprotective agent. The main goal of this review is to summarize recent findings on damaging cardiac effects of fructose-rich diet in females, mostly experimental animals, and to evaluate protective capacity of estradiol. Published results of our and other research groups indicate mostly detrimental effects of fructose-rich diet on cardiac insulin signaling molecules, glucose and fatty acid metabolism, nitric oxide production and ion transport, as well as renin-angiotensin system and inflammation. Some of these processes are involved in cardiac insulin signal transmission, others are regulated by insulin or have an influence on insulin action. Administration of estradiol to ovariectomized female rats, exposed to increased intake of fructose, was mostly beneficial to the heart, but sometimes it was ineffective or even detrimental, depending on the particular processes. We believe that these data, carefully translated to human population, could be useful for clinicians dealing with postmenopausal women susceptible to metabolic diseases and hormone replacement therapy.
T2  - European Journal of Pharmacology
T1  - Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?
VL  - 811
SP  - 141
EP  - 147
DO  - 10.1016/j.ejphar.2017.06.003
ER  - 

@article{
author = "Bundalo, Maja M. and Romić, Snježana Đ. and Tepavčević, Snežana and Stojiljković, Mojca D. and Stanković, Aleksandra and Živković, Maja and Korićanac, Goran",
year = "2017",
abstract = "Increased intake of fructose in humans and laboratory animals is demonstrated to be a risk factor for development of metabolic disorders (insulin resistance, metabolic syndrome, type 2 diabetes) and cardiovascular diseases. On the other hand, estradiol is emphasized as a cardioprotective agent. The main goal of this review is to summarize recent findings on damaging cardiac effects of fructose-rich diet in females, mostly experimental animals, and to evaluate protective capacity of estradiol. Published results of our and other research groups indicate mostly detrimental effects of fructose-rich diet on cardiac insulin signaling molecules, glucose and fatty acid metabolism, nitric oxide production and ion transport, as well as renin-angiotensin system and inflammation. Some of these processes are involved in cardiac insulin signal transmission, others are regulated by insulin or have an influence on insulin action. Administration of estradiol to ovariectomized female rats, exposed to increased intake of fructose, was mostly beneficial to the heart, but sometimes it was ineffective or even detrimental, depending on the particular processes. We believe that these data, carefully translated to human population, could be useful for clinicians dealing with postmenopausal women susceptible to metabolic diseases and hormone replacement therapy.",
journal = "European Journal of Pharmacology",
title = "Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?",
volume = "811",
pages = "141-147",
doi = "10.1016/j.ejphar.2017.06.003"
}

Bundalo, M. M., Romić, S. Đ., Tepavčević, S., Stojiljković, M. D., Stanković, A., Živković, M.,& Korićanac, G.. (2017). Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?. in European Journal of Pharmacology, 811, 141-147.
https://doi.org/10.1016/j.ejphar.2017.06.003

Bundalo MM, Romić SĐ, Tepavčević S, Stojiljković MD, Stanković A, Živković M, Korićanac G. Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?. in European Journal of Pharmacology. 2017;811:141-147.
doi:10.1016/j.ejphar.2017.06.003 .

Bundalo, Maja M., Romić, Snježana Đ., Tepavčević, Snežana, Stojiljković, Mojca D., Stanković, Aleksandra, Živković, Maja, Korićanac, Goran, "Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?" in European Journal of Pharmacology, 811 (2017):141-147,
https://doi.org/10.1016/j.ejphar.2017.06.003 . .

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Đorđević, Ana D.
AU  - Bursać, Biljana
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1839
AB  - The adipose tissue renin-angiotensin system (RAS) is proposed to be a pathophysiological link between adipose tissue dysregulation and metabolic disorders induced by a fructose-rich diet (FRD). RAS can act intracellularly. We hypothesized that adipocyte nuclear membranes possess angiotensin receptor types 1 and 2 (AT1R and AT2R), which couple to nuclear signaling pathways and regulate oxidative gene expression under FRD conditions. We analyzed the effect of consumption of 10% fructose solution for 9 weeks on biochemical parameters, adipocyte morphology, and expression of AT1R, AT2R, AT1R-associated protein (ATRAP), NADPH oxidase 4 (NOX4), matrix metalloproteinase-9 (MMP-9), and manganese superoxide dismutase (MnSOD) in adipose tissue of Wistar rats. We detected AT1R and AT2R in the nuclear fraction. FRD reduced the level of angiotensin receptors in the nucleus, while increased AT1R and decreased AT2R levels were observed in the plasma membrane. FRD increased the ATRAP mRNA level and decreased MnSOD mRNA and protein levels. No significant differences were observed for MMP-9 and NOX4 mRNA levels. These findings coincided with hyperleptinemia, elevated blood pressure and triglycerides, and unchanged visceral adipose tissue mass and morphology in FRD rats. Besides providing evidence for nuclear localization of angiotensin receptors in visceral adipose tissue, this study demonstrates the different effects of FRD on AT1R expression in different cellular compartments. Elevated blood pressure and decreased antioxidant capacity in visceral fat of fructose-fed rats were accompanied by an increased AT1R level in the plasma membrane, while upregulation of ATRAP and a decrease of nuclear membrane AT1R suggest an increased capacity for attenuation of excessive AT1R signaling and visceral adiposity.
T2  - Applied Physiology Nutrition and Metabolism
T1  - Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue
VL  - 42
IS  - 12
SP  - 1254
EP  - 1263
DO  - 10.1139/apnm-2016-0725
ER  - 

@article{
author = "Bundalo, Maja M. and Đorđević, Ana D. and Bursać, Biljana and Živković, Maja and Korićanac, Goran and Stanković, Aleksandra",
year = "2017",
abstract = "The adipose tissue renin-angiotensin system (RAS) is proposed to be a pathophysiological link between adipose tissue dysregulation and metabolic disorders induced by a fructose-rich diet (FRD). RAS can act intracellularly. We hypothesized that adipocyte nuclear membranes possess angiotensin receptor types 1 and 2 (AT1R and AT2R), which couple to nuclear signaling pathways and regulate oxidative gene expression under FRD conditions. We analyzed the effect of consumption of 10% fructose solution for 9 weeks on biochemical parameters, adipocyte morphology, and expression of AT1R, AT2R, AT1R-associated protein (ATRAP), NADPH oxidase 4 (NOX4), matrix metalloproteinase-9 (MMP-9), and manganese superoxide dismutase (MnSOD) in adipose tissue of Wistar rats. We detected AT1R and AT2R in the nuclear fraction. FRD reduced the level of angiotensin receptors in the nucleus, while increased AT1R and decreased AT2R levels were observed in the plasma membrane. FRD increased the ATRAP mRNA level and decreased MnSOD mRNA and protein levels. No significant differences were observed for MMP-9 and NOX4 mRNA levels. These findings coincided with hyperleptinemia, elevated blood pressure and triglycerides, and unchanged visceral adipose tissue mass and morphology in FRD rats. Besides providing evidence for nuclear localization of angiotensin receptors in visceral adipose tissue, this study demonstrates the different effects of FRD on AT1R expression in different cellular compartments. Elevated blood pressure and decreased antioxidant capacity in visceral fat of fructose-fed rats were accompanied by an increased AT1R level in the plasma membrane, while upregulation of ATRAP and a decrease of nuclear membrane AT1R suggest an increased capacity for attenuation of excessive AT1R signaling and visceral adiposity.",
journal = "Applied Physiology Nutrition and Metabolism",
title = "Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue",
volume = "42",
number = "12",
pages = "1254-1263",
doi = "10.1139/apnm-2016-0725"
}

Bundalo, M. M., Đorđević, A. D., Bursać, B., Živković, M., Korićanac, G.,& Stanković, A.. (2017). Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue. in Applied Physiology Nutrition and Metabolism, 42(12), 1254-1263.
https://doi.org/10.1139/apnm-2016-0725

Bundalo MM, Đorđević AD, Bursać B, Živković M, Korićanac G, Stanković A. Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue. in Applied Physiology Nutrition and Metabolism. 2017;42(12):1254-1263.
doi:10.1139/apnm-2016-0725 .

Bundalo, Maja M., Đorđević, Ana D., Bursać, Biljana, Živković, Maja, Korićanac, Goran, Stanković, Aleksandra, "Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue" in Applied Physiology Nutrition and Metabolism, 42, no. 12 (2017):1254-1263,
https://doi.org/10.1139/apnm-2016-0725 . .

TY  - JOUR
AU  - Cvetković, Mirjana
AU  - Živković, Maja
AU  - Bundalo, Maja M.
AU  - Gojković, Ivana
AU  - Spasojević-Dimitrijeva, Brankica
AU  - Stanković, Aleksandra
AU  - Kostić, Mirjana M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1918
AB  - Background: The interindividual variability of cyclosporin A (CsA) pharmacokinetics might be explained by heterogeneity in the cytochrome P450 3A (CYP3A) subfamily. Altered CYP3A enzyme activity was associated with variant allele of P450 oxidoreductase gene (POR*28). The aim of this study was to assess the impact of age, CYP3A5*3, CYP3A4*22, and POR*28 alleles on CsA pharmacokinetics in pediatric renal transplant recipients. Methods: Renal transplant patients receiving CsA (n = 47) were genotyped for CYP3A5*3, CYP3A4*22, and POR*28. Results: CYP3A5 nonexpressers had higher overall dose-adjusted predose concentration (C0/dose; ng/mL per mg/kg) compared with expressers (31.48 +/- 12.75 versus 22.44 +/- 7.12, P = 0.01). CY-P3A5 nonexpressers carrying POR*28 allele had a lower overall dose-adjusted concentration (C2/dose) than those with POR*1/*1 genotype (165.54 +/- 70.40 versus 210.55 +/- 79.98, P = 0.02), with age as covariate. Children aged 6 years and younger had a lower overall C0/dose (18.82 +/- 4.72 versus 34.19 +/- 11.89, P = 0.001) and C2/dose (106.75 +/- 26.99 versus 209.20 +/- 71.57, P LT 0.001) compared with older children. Carriers of CYP3A5*3 allele aged LT = 6 years required higher dose of CsA and achieved lower C0/dose and C2/dose, at most time points, than older carriers of this allele. Carriers of POR*28 allele aged # 6 years required higher doses of CsA, whereas they achieved lower C0/dose and C2/dose, at most time points, in comparison to older carriers of this allele. The significant effect of age (P LT 0.002) and CYP3A5 variation (P, 0.02) was shown for overall C0/dose, whereas age (P LT 0.00001) and POR variation (P = 0.05) showed significant effect on C2/dose. Regression summary for overall C2/dose in patients aged 6 years younger showed a significant effect of both CYP3A5 and POR variations (P LT 0.016). Conclusions: Younger age, POR*28 allele, and CYP3A5*3 allele were associated with higher CsA dosing requirements and lower concentration/dose ratio. Pretransplant screening of relevant polymorphisms in accordance with age should be considered to adjust therapy.
T2  - Therapeutic Drug Monitoring
T1  - Effect of Age and Allele Variants of CYP3A5, CYP3A4, and POR Genes on the Pharmacokinetics of Cyclosporin A in Pediatric Renal Transplant Recipients From Serbia
VL  - 39
IS  - 6
SP  - 589
EP  - 595
DO  - 10.1097/FTD.0000000000000442
ER  - 

@article{
author = "Cvetković, Mirjana and Živković, Maja and Bundalo, Maja M. and Gojković, Ivana and Spasojević-Dimitrijeva, Brankica and Stanković, Aleksandra and Kostić, Mirjana M.",
year = "2017",
abstract = "Background: The interindividual variability of cyclosporin A (CsA) pharmacokinetics might be explained by heterogeneity in the cytochrome P450 3A (CYP3A) subfamily. Altered CYP3A enzyme activity was associated with variant allele of P450 oxidoreductase gene (POR*28). The aim of this study was to assess the impact of age, CYP3A5*3, CYP3A4*22, and POR*28 alleles on CsA pharmacokinetics in pediatric renal transplant recipients. Methods: Renal transplant patients receiving CsA (n = 47) were genotyped for CYP3A5*3, CYP3A4*22, and POR*28. Results: CYP3A5 nonexpressers had higher overall dose-adjusted predose concentration (C0/dose; ng/mL per mg/kg) compared with expressers (31.48 +/- 12.75 versus 22.44 +/- 7.12, P = 0.01). CY-P3A5 nonexpressers carrying POR*28 allele had a lower overall dose-adjusted concentration (C2/dose) than those with POR*1/*1 genotype (165.54 +/- 70.40 versus 210.55 +/- 79.98, P = 0.02), with age as covariate. Children aged 6 years and younger had a lower overall C0/dose (18.82 +/- 4.72 versus 34.19 +/- 11.89, P = 0.001) and C2/dose (106.75 +/- 26.99 versus 209.20 +/- 71.57, P LT 0.001) compared with older children. Carriers of CYP3A5*3 allele aged LT = 6 years required higher dose of CsA and achieved lower C0/dose and C2/dose, at most time points, than older carriers of this allele. Carriers of POR*28 allele aged # 6 years required higher doses of CsA, whereas they achieved lower C0/dose and C2/dose, at most time points, in comparison to older carriers of this allele. The significant effect of age (P LT 0.002) and CYP3A5 variation (P, 0.02) was shown for overall C0/dose, whereas age (P LT 0.00001) and POR variation (P = 0.05) showed significant effect on C2/dose. Regression summary for overall C2/dose in patients aged 6 years younger showed a significant effect of both CYP3A5 and POR variations (P LT 0.016). Conclusions: Younger age, POR*28 allele, and CYP3A5*3 allele were associated with higher CsA dosing requirements and lower concentration/dose ratio. Pretransplant screening of relevant polymorphisms in accordance with age should be considered to adjust therapy.",
journal = "Therapeutic Drug Monitoring",
title = "Effect of Age and Allele Variants of CYP3A5, CYP3A4, and POR Genes on the Pharmacokinetics of Cyclosporin A in Pediatric Renal Transplant Recipients From Serbia",
volume = "39",
number = "6",
pages = "589-595",
doi = "10.1097/FTD.0000000000000442"
}

Cvetković, M., Živković, M., Bundalo, M. M., Gojković, I., Spasojević-Dimitrijeva, B., Stanković, A.,& Kostić, M. M.. (2017). Effect of Age and Allele Variants of CYP3A5, CYP3A4, and POR Genes on the Pharmacokinetics of Cyclosporin A in Pediatric Renal Transplant Recipients From Serbia. in Therapeutic Drug Monitoring, 39(6), 589-595.
https://doi.org/10.1097/FTD.0000000000000442

Cvetković M, Živković M, Bundalo MM, Gojković I, Spasojević-Dimitrijeva B, Stanković A, Kostić MM. Effect of Age and Allele Variants of CYP3A5, CYP3A4, and POR Genes on the Pharmacokinetics of Cyclosporin A in Pediatric Renal Transplant Recipients From Serbia. in Therapeutic Drug Monitoring. 2017;39(6):589-595.
doi:10.1097/FTD.0000000000000442 .

Cvetković, Mirjana, Živković, Maja, Bundalo, Maja M., Gojković, Ivana, Spasojević-Dimitrijeva, Brankica, Stanković, Aleksandra, Kostić, Mirjana M., "Effect of Age and Allele Variants of CYP3A5, CYP3A4, and POR Genes on the Pharmacokinetics of Cyclosporin A in Pediatric Renal Transplant Recipients From Serbia" in Therapeutic Drug Monitoring, 39, no. 6 (2017):589-595,
https://doi.org/10.1097/FTD.0000000000000442 . .

TY  - CONF
AU  - Bošković, Maja
AU  - Bundalo, Maja M.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7179
C3  - Atherosclerosis
T1  - Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress
VL  - 263
SP  - E192
EP  - E192
DO  - 10.1016/j.atherosclerosis.2017.06.616
ER  - 

@conference{
author = "Bošković, Maja and Bundalo, Maja M. and Stojiljković, Mojca D. and Kostić, Milan and Živković, Maja and Korićanac, Goran and Stanković, Aleksandra and Životić, Ivan",
year = "2017",
journal = "Atherosclerosis",
title = "Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress",
volume = "263",
pages = "E192-E192",
doi = "10.1016/j.atherosclerosis.2017.06.616"
}

Bošković, M., Bundalo, M. M., Stojiljković, M. D., Kostić, M., Živković, M., Korićanac, G., Stanković, A.,& Životić, I.. (2017). Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress. in Atherosclerosis, 263, E192-E192.
https://doi.org/10.1016/j.atherosclerosis.2017.06.616

Bošković M, Bundalo MM, Stojiljković MD, Kostić M, Živković M, Korićanac G, Stanković A, Životić I. Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress. in Atherosclerosis. 2017;263:E192-E192.
doi:10.1016/j.atherosclerosis.2017.06.616 .

Bošković, Maja, Bundalo, Maja M., Stojiljković, Mojca D., Kostić, Milan, Živković, Maja, Korićanac, Goran, Stanković, Aleksandra, Životić, Ivan, "Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress" in Atherosclerosis, 263 (2017):E192-E192,
https://doi.org/10.1016/j.atherosclerosis.2017.06.616 . .

TY  - JOUR
AU  - Stanković, Aleksandra
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Bundalo, Maja M.
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1045
AB  - Background and Aims: The principal biologic effects of the renin-angiotensin system are mediated by activation of the AT1R receptor. The microRNA miR-155 regulates AT1R expression, with both its, and AT1Rs activity, linked to atherosclerosis. Target sites for miR-155 lie within the 3 UTR of the human AT1R gene, and include the AT1R A1166C polymorphism. Thus far, only levels of circulating miR-155 have been investigated with respect to A1166C genotypes. We hypothesized that the A1166C polymorphism could correlate with different, ultra-sonographically defined plaque phenotypes, as well as with an altered expression of AT1R mRNA and protein in human carotid plaques (CP), and altered expression of miR-155 in patients with advanced atherosclerosis. Methods: Our study cohort comprised 411 patients with advanced carotid atherosclerosis (298 hyperechoic; 113 hypoechoic plaques). PCR analyses identified A1166C genotypes; quantitative real-time PCR determined AT1R and miR-155 expression levels, with AT1R protein expression evaluated by western blot. Results: Genotypes containing the C allele bore a significant association with the hypoechoic plaque phenotype (adjusted OR 1.87, 95% CI 1.16-3.00, p = 0.01). The expression of AT1R mRNA and miR-155 were significantly up-regulated in the CPs of CC genotype carriers compared to the AA/AC genotypes (p = 0.032, p = 0.015, respectively). AT1R protein expression was also significantly higher for CC genotypes (p LT 0.01). Conclusion: Our results indicate that the AT1R A1166C polymorphism impacts an ultrasonographicallydefined human plaque phenotype, with intra-plaque AT1R and miR-155 expression altered in advanced carotid atherosclerosis. Validation and replication of these data should contribute to an improved personalized therapy with which to prevent carotid atherosclerosis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Atherosclerosis
T1  - Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques
VL  - 248
SP  - 132
EP  - 139
DO  - 10.1016/j.atherosclerosis.2016.02.032
ER  - 

@article{
author = "Stanković, Aleksandra and Kolaković, Ana and Živković, Maja and Đurić, Tamara and Bundalo, Maja M. and Končar, Igor and Davidović, Lazar and Alavantić, Dragan",
year = "2016",
abstract = "Background and Aims: The principal biologic effects of the renin-angiotensin system are mediated by activation of the AT1R receptor. The microRNA miR-155 regulates AT1R expression, with both its, and AT1Rs activity, linked to atherosclerosis. Target sites for miR-155 lie within the 3 UTR of the human AT1R gene, and include the AT1R A1166C polymorphism. Thus far, only levels of circulating miR-155 have been investigated with respect to A1166C genotypes. We hypothesized that the A1166C polymorphism could correlate with different, ultra-sonographically defined plaque phenotypes, as well as with an altered expression of AT1R mRNA and protein in human carotid plaques (CP), and altered expression of miR-155 in patients with advanced atherosclerosis. Methods: Our study cohort comprised 411 patients with advanced carotid atherosclerosis (298 hyperechoic; 113 hypoechoic plaques). PCR analyses identified A1166C genotypes; quantitative real-time PCR determined AT1R and miR-155 expression levels, with AT1R protein expression evaluated by western blot. Results: Genotypes containing the C allele bore a significant association with the hypoechoic plaque phenotype (adjusted OR 1.87, 95% CI 1.16-3.00, p = 0.01). The expression of AT1R mRNA and miR-155 were significantly up-regulated in the CPs of CC genotype carriers compared to the AA/AC genotypes (p = 0.032, p = 0.015, respectively). AT1R protein expression was also significantly higher for CC genotypes (p LT 0.01). Conclusion: Our results indicate that the AT1R A1166C polymorphism impacts an ultrasonographicallydefined human plaque phenotype, with intra-plaque AT1R and miR-155 expression altered in advanced carotid atherosclerosis. Validation and replication of these data should contribute to an improved personalized therapy with which to prevent carotid atherosclerosis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Atherosclerosis",
title = "Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques",
volume = "248",
pages = "132-139",
doi = "10.1016/j.atherosclerosis.2016.02.032"
}

Stanković, A., Kolaković, A., Živković, M., Đurić, T., Bundalo, M. M., Končar, I., Davidović, L.,& Alavantić, D.. (2016). Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques. in Atherosclerosis
Elsevier., 248, 132-139.
https://doi.org/10.1016/j.atherosclerosis.2016.02.032

Stanković A, Kolaković A, Živković M, Đurić T, Bundalo MM, Končar I, Davidović L, Alavantić D. Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques. in Atherosclerosis. 2016;248:132-139.
doi:10.1016/j.atherosclerosis.2016.02.032 .

Stanković, Aleksandra, Kolaković, Ana, Živković, Maja, Đurić, Tamara, Bundalo, Maja M., Končar, Igor, Davidović, Lazar, Alavantić, Dragan, "Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques" in Atherosclerosis, 248 (2016):132-139,
https://doi.org/10.1016/j.atherosclerosis.2016.02.032 . .

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Korićanac, Goran
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1132
AB  - Introduction: The cardiovascular renin-angiotensin system (RAS) could be affected by gender and dietary regime. We hypothesized that male rats will be more susceptible to activation of RAS in the heart and aorta, as a response to a fructose-rich diet (FRD). Materials and methods: Both male and female Wistar rats were given a 10% (w/v) fructose solution for 9 weeks. We measured the biochemical parameters, blood pressure (BP) and heart rate. We used Western blot and real-time polymerase chain reaction (PCR) to quantify protein and gene expression. Results: In the male rats, the FRD elevated BP and expression of cardiac angiotensin-converting enzyme (ACE), while the expression of angiotensin-converting enzyme 2 (ACE2) and angiotensin II Type 2 receptor (AT(2)R) were significantly decreased. In female rats, there were no changes in cardiac RAS expression due to FRD. Furthermore, the ACE/AT(1)R axis was overexpressed in the FRD male rats aortae, while only AT(1)R was upregulated in the FRD female rats aortae. ACE2 expression remained unchanged in the aortae of both genders receiving the FRD. Conclusions: The FRD induced gender-specific changes in the expression of the RAS in the heart and aortae of male rats. Further investigations are required in order to get a comprehensive understanding of the underlying mechanisms of gender-specific fructose-induced cardiovascular pathologies.
T2  - Journal of the Renin-Angiotensin-Aldosterone System
T1  - Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta
VL  - 17
IS  - 2
DO  - 10.1177/1470320316642915
ER  - 

@article{
author = "Bundalo, Maja M. and Živković, Maja and Romić, Snježana Đ. and Tepavčević, Snežana and Korićanac, Goran and Đurić, Tamara and Stanković, Aleksandra",
year = "2016",
abstract = "Introduction: The cardiovascular renin-angiotensin system (RAS) could be affected by gender and dietary regime. We hypothesized that male rats will be more susceptible to activation of RAS in the heart and aorta, as a response to a fructose-rich diet (FRD). Materials and methods: Both male and female Wistar rats were given a 10% (w/v) fructose solution for 9 weeks. We measured the biochemical parameters, blood pressure (BP) and heart rate. We used Western blot and real-time polymerase chain reaction (PCR) to quantify protein and gene expression. Results: In the male rats, the FRD elevated BP and expression of cardiac angiotensin-converting enzyme (ACE), while the expression of angiotensin-converting enzyme 2 (ACE2) and angiotensin II Type 2 receptor (AT(2)R) were significantly decreased. In female rats, there were no changes in cardiac RAS expression due to FRD. Furthermore, the ACE/AT(1)R axis was overexpressed in the FRD male rats aortae, while only AT(1)R was upregulated in the FRD female rats aortae. ACE2 expression remained unchanged in the aortae of both genders receiving the FRD. Conclusions: The FRD induced gender-specific changes in the expression of the RAS in the heart and aortae of male rats. Further investigations are required in order to get a comprehensive understanding of the underlying mechanisms of gender-specific fructose-induced cardiovascular pathologies.",
journal = "Journal of the Renin-Angiotensin-Aldosterone System",
title = "Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta",
volume = "17",
number = "2",
doi = "10.1177/1470320316642915"
}

Bundalo, M. M., Živković, M., Romić, S. Đ., Tepavčević, S., Korićanac, G., Đurić, T.,& Stanković, A.. (2016). Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta. in Journal of the Renin-Angiotensin-Aldosterone System, 17(2).
https://doi.org/10.1177/1470320316642915

Bundalo MM, Živković M, Romić SĐ, Tepavčević S, Korićanac G, Đurić T, Stanković A. Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta. in Journal of the Renin-Angiotensin-Aldosterone System. 2016;17(2).
doi:10.1177/1470320316642915 .

Bundalo, Maja M., Živković, Maja, Romić, Snježana Đ., Tepavčević, Snežana, Korićanac, Goran, Đurić, Tamara, Stanković, Aleksandra, "Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta" in Journal of the Renin-Angiotensin-Aldosterone System, 17, no. 2 (2016),
https://doi.org/10.1177/1470320316642915 . .

TY  - CONF
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7133
C3  - Atherosclerosis
T1  - Estradiol Protects Ovariectomized Female Rats Against Fructose Rich Diet Induced Cardiac Inflammation
VL  - 252
SP  - E207
EP  - E207
DO  - 10.1016/j.atherosclerosis.2016.07.139
ER  - 

@conference{
author = "Bundalo, Maja M. and Živković, Maja and Korićanac, Goran and Stanković, Aleksandra",
year = "2016",
journal = "Atherosclerosis",
title = "Estradiol Protects Ovariectomized Female Rats Against Fructose Rich Diet Induced Cardiac Inflammation",
volume = "252",
pages = "E207-E207",
doi = "10.1016/j.atherosclerosis.2016.07.139"
}

Bundalo, M. M., Živković, M., Korićanac, G.,& Stanković, A.. (2016). Estradiol Protects Ovariectomized Female Rats Against Fructose Rich Diet Induced Cardiac Inflammation. in Atherosclerosis, 252, E207-E207.
https://doi.org/10.1016/j.atherosclerosis.2016.07.139

Bundalo MM, Živković M, Korićanac G, Stanković A. Estradiol Protects Ovariectomized Female Rats Against Fructose Rich Diet Induced Cardiac Inflammation. in Atherosclerosis. 2016;252:E207-E207.
doi:10.1016/j.atherosclerosis.2016.07.139 .

Bundalo, Maja M., Živković, Maja, Korićanac, Goran, Stanković, Aleksandra, "Estradiol Protects Ovariectomized Female Rats Against Fructose Rich Diet Induced Cardiac Inflammation" in Atherosclerosis, 252 (2016):E207-E207,
https://doi.org/10.1016/j.atherosclerosis.2016.07.139 . .

TY  - THES
AU  - Bundalo, Maja M.
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=3043
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:11284/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025057202
UR  - http://nardus.mpn.gov.rs/123456789/5664
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7279
AB  - Ishrana bogata fruktozom predstavlja bitan faktor u razvoju metaboličkogsindroma koji je povezan sa povećanim rizikom za nastanak kardiovaskularnih bolesti.Smatra se da hronična inflamacija ima bitnu ulogu u njegovoj patogenezi. Ženke suzaštićene od ishranom izazvanih metaboličkih poremećaja i hipertenzije ureproduktivnom periodu. Estrogen, koji utiče na normalno funkcionisanjekardiovaskularnog sistema, bi mogao doprinositi uočenim polno specifičnim razlikama unastanku simptoma metaboličkog sindroma. Proteinske komponente renin-angiotenzinsistema (RAS) imaju bitnu ulogu u nastanku inflamacije, hipertenzije i insulinskerezistencije i na njihovu ekspresiju utiče estradiol. Ova doktorska disertacija je za ciljimala da ispita da li pol i estradiol doprinose promenama u ekspresiji komponenti RAS-a[angiotenzin konvertujućeg enzima (ACE), angiotenzin konvertujućeg enzima 2 (ACE2),angiotenzinskog receptora tipa 1 (AT1R), angiotenzinskog receptora tipa 2 (AT2R) ikolektrina] i medijatora inflamacije i remodelovanja tkiva srca [nuklearnog faktora κB(NFκB), matriks metaloproteinaze-9 (MMP-9) i liganda 16 iz familije hemokina CXC(CXCL16)] kod animalnog modela metaboličkog sindroma.Mužjaci i ženke pacova koji su pili 10% rastvor fruktoze umesto vode u trajanjuod 9 nedelja predstavljali su animalni model metaboličkog sindroma korišćen u ovojstudiji. Radi ispitivanja efekata estradiola jedan deo ženki pacova je ovarijektomisan ipodeljen u tri grupe pri čemi je jedna grupa pored standardne laboratorijske hrane pilavodu, druga grupa je pila 10% rastvor fruktoze, a treća grupa je pored rasvora fruktozeprimala i supstitucionu terapiju estradiolom. Ishrana bogata fruktozom je uzrokovalapovećanje krvnog pritiska samo kod mužjaka pacova. Ovaj tip ishrane nije doveo dopromena u masi srca niti u odnosu masa srca/masa tela ni kod jednog pola, kao ni kodovarijektomisanih životinja što ukazuje da se hipertrofija srca nije razvila...
AB  - Fructose rich diet (FRD) represents an important factor in the development ofmetabolic syndrome. Metabolic syndrome is associated with increased risk forcardiovascular diseases occurrence and chronic inflammation has a major role in itspathogenesis. Females are protected from diet-induced metabolic disturbances andhypertension in their reproductive period. Estrogen, which influences the cardiovascularsystem, could contribute to the observed gender-specific differences in the onset ofmetabolic syndrome. Components of the renin-angiotensin system (RAS), the synthesisof which is mediated by estrogen, have an important role in the inflammatory processes,hypertension and insulin resistance. Almost all of the components of metabolic syndromeincrease the activity of RAS, which finally results in increased oxidative stress andinflammation. The aim of this doctoral dissertation was to investigate sex specific changesand role of estradiol in the expression of RAS components [angiotensin convertingenzyme (ACE), angiotensin converting enzyme 2 (ACE2), angiotensin receptor type 1(AT1R), angiotensin receptor type 2 (AT2R) and collectrin], as well as mediators ofinflammation and remodeling of heart tissue [nuclear faktor κB (NFκB), matrixmetalloproteinse-9 (MMP-9) and CXCL16 chemokine] in the animal model of metabolicsyndrome.Male and female rats, which consumed 10% fructose solution instead of water for9 weeks, represent an animal model of metabolic syndrome that was used in this study. Inorder to examine the effects of estradiol in the context of FRD, female rats wereovariectomized and divided in three groups: fed normal diet, fed FRD, and fed FRD andsubjected to estradiol replacement therapy. FRD increased blood pressure only in malerats. This diet regime didn't cause heart hypertrophy neither in intact males and females,nor in ovariectomized females...
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Uticaj ishrane bogate fruktozom na ekspresiju komponenti renin-angiotenzin sistema i inflamacije u tkivu srca pacova: polno specifične razlike
T1  - The effect of fructose rich diet on the expression of renin-angiostensin system components and inflamatory molecules in the rat heart: sex specific differences
UR  - https://hdl.handle.net/21.15107/rcub_nardus_5664
ER  - 

@phdthesis{
author = "Bundalo, Maja M.",
year = "2016",
abstract = "Ishrana bogata fruktozom predstavlja bitan faktor u razvoju metaboličkogsindroma koji je povezan sa povećanim rizikom za nastanak kardiovaskularnih bolesti.Smatra se da hronična inflamacija ima bitnu ulogu u njegovoj patogenezi. Ženke suzaštićene od ishranom izazvanih metaboličkih poremećaja i hipertenzije ureproduktivnom periodu. Estrogen, koji utiče na normalno funkcionisanjekardiovaskularnog sistema, bi mogao doprinositi uočenim polno specifičnim razlikama unastanku simptoma metaboličkog sindroma. Proteinske komponente renin-angiotenzinsistema (RAS) imaju bitnu ulogu u nastanku inflamacije, hipertenzije i insulinskerezistencije i na njihovu ekspresiju utiče estradiol. Ova doktorska disertacija je za ciljimala da ispita da li pol i estradiol doprinose promenama u ekspresiji komponenti RAS-a[angiotenzin konvertujućeg enzima (ACE), angiotenzin konvertujućeg enzima 2 (ACE2),angiotenzinskog receptora tipa 1 (AT1R), angiotenzinskog receptora tipa 2 (AT2R) ikolektrina] i medijatora inflamacije i remodelovanja tkiva srca [nuklearnog faktora κB(NFκB), matriks metaloproteinaze-9 (MMP-9) i liganda 16 iz familije hemokina CXC(CXCL16)] kod animalnog modela metaboličkog sindroma.Mužjaci i ženke pacova koji su pili 10% rastvor fruktoze umesto vode u trajanjuod 9 nedelja predstavljali su animalni model metaboličkog sindroma korišćen u ovojstudiji. Radi ispitivanja efekata estradiola jedan deo ženki pacova je ovarijektomisan ipodeljen u tri grupe pri čemi je jedna grupa pored standardne laboratorijske hrane pilavodu, druga grupa je pila 10% rastvor fruktoze, a treća grupa je pored rasvora fruktozeprimala i supstitucionu terapiju estradiolom. Ishrana bogata fruktozom je uzrokovalapovećanje krvnog pritiska samo kod mužjaka pacova. Ovaj tip ishrane nije doveo dopromena u masi srca niti u odnosu masa srca/masa tela ni kod jednog pola, kao ni kodovarijektomisanih životinja što ukazuje da se hipertrofija srca nije razvila..., Fructose rich diet (FRD) represents an important factor in the development ofmetabolic syndrome. Metabolic syndrome is associated with increased risk forcardiovascular diseases occurrence and chronic inflammation has a major role in itspathogenesis. Females are protected from diet-induced metabolic disturbances andhypertension in their reproductive period. Estrogen, which influences the cardiovascularsystem, could contribute to the observed gender-specific differences in the onset ofmetabolic syndrome. Components of the renin-angiotensin system (RAS), the synthesisof which is mediated by estrogen, have an important role in the inflammatory processes,hypertension and insulin resistance. Almost all of the components of metabolic syndromeincrease the activity of RAS, which finally results in increased oxidative stress andinflammation. The aim of this doctoral dissertation was to investigate sex specific changesand role of estradiol in the expression of RAS components [angiotensin convertingenzyme (ACE), angiotensin converting enzyme 2 (ACE2), angiotensin receptor type 1(AT1R), angiotensin receptor type 2 (AT2R) and collectrin], as well as mediators ofinflammation and remodeling of heart tissue [nuclear faktor κB (NFκB), matrixmetalloproteinse-9 (MMP-9) and CXCL16 chemokine] in the animal model of metabolicsyndrome.Male and female rats, which consumed 10% fructose solution instead of water for9 weeks, represent an animal model of metabolic syndrome that was used in this study. Inorder to examine the effects of estradiol in the context of FRD, female rats wereovariectomized and divided in three groups: fed normal diet, fed FRD, and fed FRD andsubjected to estradiol replacement therapy. FRD increased blood pressure only in malerats. This diet regime didn't cause heart hypertrophy neither in intact males and females,nor in ovariectomized females...",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Uticaj ishrane bogate fruktozom na ekspresiju komponenti renin-angiotenzin sistema i inflamacije u tkivu srca pacova: polno specifične razlike, The effect of fructose rich diet on the expression of renin-angiostensin system components and inflamatory molecules in the rat heart: sex specific differences",
url = "https://hdl.handle.net/21.15107/rcub_nardus_5664"
}

Bundalo, M. M.. (2016). Uticaj ishrane bogate fruktozom na ekspresiju komponenti renin-angiotenzin sistema i inflamacije u tkivu srca pacova: polno specifične razlike. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_5664

Bundalo MM. Uticaj ishrane bogate fruktozom na ekspresiju komponenti renin-angiotenzin sistema i inflamacije u tkivu srca pacova: polno specifične razlike. in Универзитет у Београду. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_5664 .

Bundalo, Maja M., "Uticaj ishrane bogate fruktozom na ekspresiju komponenti renin-angiotenzin sistema i inflamacije u tkivu srca pacova: polno specifične razlike" in Универзитет у Београду (2016),
https://hdl.handle.net/21.15107/rcub_nardus_5664 .

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/570
AB  - The renin-angiotensin system has been implicated in the development of metabolic syndrome and appears to be a key in the local tissue control of normal cardiac functions. Physiological concentrations of estrogens have been shown to be cardioprotective, especially against the damaging effects of fructose-rich diet. The aim of the study was to investigate the expression of the renin-angiotensin system molecules with potentially deleterious effect on the heart (angiotensin-converting enzyme and angiotensin II type 1 receptor) and those with potentially protective effects, (angiotensin-converting enzyme 2 and angiotensin II type 2 receptor), in ovariectomized fructose fed female rats with 17-estradiol replacement. Real-time PCR and Western blot analysis were used for quantification of gene and protein expression in the heart. Fructose diet increased the expression of angiotensin-converting enzyme and angiotensin II type 1 receptor and decreased the expression of angiotensin-converting enzyme 2 and angiotensin II type 2 receptor. On the other hand, estradiol replacement seems to undo fructose diet effects on cardiac renin-angiotensin system. Downregulation of angiotensin-converting enzyme and angiotensin II type 1 receptor, and reversion of expression of both potentially protective molecules, angiotensin-converting enzyme 2 and angiotensin II type 2 receptor, to the control level in cardiac tissue took place. Obtained results suggest that estradiol may reverse the harmful effect of fructose-rich diet on the expression of renin-angiotensin system molecules. These findings may also be important in further research of phenotypes like insulin resistance, metabolic syndrome, and following cardiovascular pathology in females.
T2  - Hormone and Metabolic Research
T1  - Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol
VL  - 47
IS  - 7
SP  - 521
EP  - 527
DO  - 10.1055/s-0034-1394373
ER  - 

@article{
author = "Bundalo, Maja M. and Živković, Maja and Tepavčević, Snežana and Ćulafić, Tijana and Korićanac, Goran and Stanković, Aleksandra",
year = "2015",
abstract = "The renin-angiotensin system has been implicated in the development of metabolic syndrome and appears to be a key in the local tissue control of normal cardiac functions. Physiological concentrations of estrogens have been shown to be cardioprotective, especially against the damaging effects of fructose-rich diet. The aim of the study was to investigate the expression of the renin-angiotensin system molecules with potentially deleterious effect on the heart (angiotensin-converting enzyme and angiotensin II type 1 receptor) and those with potentially protective effects, (angiotensin-converting enzyme 2 and angiotensin II type 2 receptor), in ovariectomized fructose fed female rats with 17-estradiol replacement. Real-time PCR and Western blot analysis were used for quantification of gene and protein expression in the heart. Fructose diet increased the expression of angiotensin-converting enzyme and angiotensin II type 1 receptor and decreased the expression of angiotensin-converting enzyme 2 and angiotensin II type 2 receptor. On the other hand, estradiol replacement seems to undo fructose diet effects on cardiac renin-angiotensin system. Downregulation of angiotensin-converting enzyme and angiotensin II type 1 receptor, and reversion of expression of both potentially protective molecules, angiotensin-converting enzyme 2 and angiotensin II type 2 receptor, to the control level in cardiac tissue took place. Obtained results suggest that estradiol may reverse the harmful effect of fructose-rich diet on the expression of renin-angiotensin system molecules. These findings may also be important in further research of phenotypes like insulin resistance, metabolic syndrome, and following cardiovascular pathology in females.",
journal = "Hormone and Metabolic Research",
title = "Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol",
volume = "47",
number = "7",
pages = "521-527",
doi = "10.1055/s-0034-1394373"
}

Bundalo, M. M., Živković, M., Tepavčević, S., Ćulafić, T., Korićanac, G.,& Stanković, A.. (2015). Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol. in Hormone and Metabolic Research, 47(7), 521-527.
https://doi.org/10.1055/s-0034-1394373

Bundalo MM, Živković M, Tepavčević S, Ćulafić T, Korićanac G, Stanković A. Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol. in Hormone and Metabolic Research. 2015;47(7):521-527.
doi:10.1055/s-0034-1394373 .

Bundalo, Maja M., Živković, Maja, Tepavčević, Snežana, Ćulafić, Tijana, Korićanac, Goran, Stanković, Aleksandra, "Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol" in Hormone and Metabolic Research, 47, no. 7 (2015):521-527,
https://doi.org/10.1055/s-0034-1394373 . .

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1112
AB  - Fructose-rich diet induces metabolic changes similar to those observed in metabolic syndrome. Among other matrix metalloproteinases, MMP-9 has an important role in adverse cardiac remodelling and might have a role in the development of cardiovascular disorders associated with metabolic syndrome. The changes of MMP-9 expression could be mediated via the NF kappa B pathway. In this study we investigated the effect of fructose-rich diet on MMP-9 expression in the heart of male and female rats, along with the effect of fructose-rich diet and oestradiol on MMP-9 expression in ovariectomized females. We further assessed the effect of fructose-rich diet and oestradiol on NF kappa B activation, measured as the level of p65 phosphorylation at Ser 276. The results showed that the diet regime did not affect the heart mass. Higher MMP-9 gene expression was found in cardiac tissue of male rats fed the fructose-rich diet than in females on the same diet regime. In ovariectomized females, fructose-rich diet upregulated MMP-9 protein and mRNA expression in the heart, as well as phosphorylation of the p65 subunit of NF kappa B at Ser 276. Oestradiol replacement therapy reverted these changes in the heart of ovariectomized females. This study has shown that oestradiol could revert the early molecular changes in MMP-9 expression induced by fructose-rich diet that occurred before cardiac hypertrophy development by decreasing phosphorylation of the NF kappa B p65 subunit at Ser 276.
T2  - Folia Biologica
T1  - Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation
VL  - 61
IS  - 6
SP  - 233
EP  - 240
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1112
ER  - 

@article{
author = "Bundalo, Maja M. and Živković, Maja and Ćulafić, Tijana and Stojiljković, Mojca D. and Korićanac, Goran and Stanković, Aleksandra",
year = "2015",
abstract = "Fructose-rich diet induces metabolic changes similar to those observed in metabolic syndrome. Among other matrix metalloproteinases, MMP-9 has an important role in adverse cardiac remodelling and might have a role in the development of cardiovascular disorders associated with metabolic syndrome. The changes of MMP-9 expression could be mediated via the NF kappa B pathway. In this study we investigated the effect of fructose-rich diet on MMP-9 expression in the heart of male and female rats, along with the effect of fructose-rich diet and oestradiol on MMP-9 expression in ovariectomized females. We further assessed the effect of fructose-rich diet and oestradiol on NF kappa B activation, measured as the level of p65 phosphorylation at Ser 276. The results showed that the diet regime did not affect the heart mass. Higher MMP-9 gene expression was found in cardiac tissue of male rats fed the fructose-rich diet than in females on the same diet regime. In ovariectomized females, fructose-rich diet upregulated MMP-9 protein and mRNA expression in the heart, as well as phosphorylation of the p65 subunit of NF kappa B at Ser 276. Oestradiol replacement therapy reverted these changes in the heart of ovariectomized females. This study has shown that oestradiol could revert the early molecular changes in MMP-9 expression induced by fructose-rich diet that occurred before cardiac hypertrophy development by decreasing phosphorylation of the NF kappa B p65 subunit at Ser 276.",
journal = "Folia Biologica",
title = "Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation",
volume = "61",
number = "6",
pages = "233-240",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1112"
}

Bundalo, M. M., Živković, M., Ćulafić, T., Stojiljković, M. D., Korićanac, G.,& Stanković, A.. (2015). Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation. in Folia Biologica, 61(6), 233-240.
https://hdl.handle.net/21.15107/rcub_vinar_1112

Bundalo MM, Živković M, Ćulafić T, Stojiljković MD, Korićanac G, Stanković A. Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation. in Folia Biologica. 2015;61(6):233-240.
https://hdl.handle.net/21.15107/rcub_vinar_1112 .

Bundalo, Maja M., Živković, Maja, Ćulafić, Tijana, Stojiljković, Mojca D., Korićanac, Goran, Stanković, Aleksandra, "Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation" in Folia Biologica, 61, no. 6 (2015):233-240,
https://hdl.handle.net/21.15107/rcub_vinar_1112 .

TY  - JOUR
AU  - Lukić, Nikola
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Bundalo, Maja M.
AU  - Krsmanovic, Z.
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5506
AB  - The function of peroxisome proliferator-activated receptor gamma (PPAR gamma) in immune regulation, as well as in anti-inflammatory and anti-proliferative actions towards T lymphocytes, has been reported. A potential role of PPARs in multiple sclerosis (MS) was suggested. The aim of this study was to investigate if there is an association of PPAR gamma-2 Pro12Ala polymorphism with MS in 361 patients from Serbia. The genotype and allele frequencies of Pro12Ala polymorphism were not significantly different between controls and patients, or between females and males. In contrast to controls, we detected a rare Ala/Ala genotype in patients with MS. We found that there is a significant association of Ala/Ala genotype with older age at onset (ANOVA, p=0.07; LSD post-hoc, Ala/Ala vs. Pro/Ala, p=0.03, Ala/Ala vs. Pro/Pro p=0.02). It would be useful to validate our results in other populations, as well as to perform follow-up of the disease progression in regard to PPAR gamma genotypes.
T2  - Archives of Biological Sciences
T1  - The Ala/Ala Genotype of Pparγ Pro12 Ala Polymorphism Is Associated with Late Onset of Multiple Sclerosis
VL  - 65
IS  - 2
SP  - 447
EP  - 453
DO  - 10.2298/ABS1302447L
ER  - 

@article{
author = "Lukić, Nikola and Stanković, Aleksandra and Dinčić, Evica and Bundalo, Maja M. and Krsmanovic, Z. and Alavantić, Dragan and Živković, Maja",
year = "2013",
abstract = "The function of peroxisome proliferator-activated receptor gamma (PPAR gamma) in immune regulation, as well as in anti-inflammatory and anti-proliferative actions towards T lymphocytes, has been reported. A potential role of PPARs in multiple sclerosis (MS) was suggested. The aim of this study was to investigate if there is an association of PPAR gamma-2 Pro12Ala polymorphism with MS in 361 patients from Serbia. The genotype and allele frequencies of Pro12Ala polymorphism were not significantly different between controls and patients, or between females and males. In contrast to controls, we detected a rare Ala/Ala genotype in patients with MS. We found that there is a significant association of Ala/Ala genotype with older age at onset (ANOVA, p=0.07; LSD post-hoc, Ala/Ala vs. Pro/Ala, p=0.03, Ala/Ala vs. Pro/Pro p=0.02). It would be useful to validate our results in other populations, as well as to perform follow-up of the disease progression in regard to PPAR gamma genotypes.",
journal = "Archives of Biological Sciences",
title = "The Ala/Ala Genotype of Pparγ Pro12 Ala Polymorphism Is Associated with Late Onset of Multiple Sclerosis",
volume = "65",
number = "2",
pages = "447-453",
doi = "10.2298/ABS1302447L"
}

Lukić, N., Stanković, A., Dinčić, E., Bundalo, M. M., Krsmanovic, Z., Alavantić, D.,& Živković, M.. (2013). The Ala/Ala Genotype of Pparγ Pro12 Ala Polymorphism Is Associated with Late Onset of Multiple Sclerosis. in Archives of Biological Sciences, 65(2), 447-453.
https://doi.org/10.2298/ABS1302447L

Lukić N, Stanković A, Dinčić E, Bundalo MM, Krsmanovic Z, Alavantić D, Živković M. The Ala/Ala Genotype of Pparγ Pro12 Ala Polymorphism Is Associated with Late Onset of Multiple Sclerosis. in Archives of Biological Sciences. 2013;65(2):447-453.
doi:10.2298/ABS1302447L .

Lukić, Nikola, Stanković, Aleksandra, Dinčić, Evica, Bundalo, Maja M., Krsmanovic, Z., Alavantić, Dragan, Živković, Maja, "The Ala/Ala Genotype of Pparγ Pro12 Ala Polymorphism Is Associated with Late Onset of Multiple Sclerosis" in Archives of Biological Sciences, 65, no. 2 (2013):447-453,
https://doi.org/10.2298/ABS1302447L . .

TY  - CONF
AU  - Bundalo, Maja M.
AU  - Tepavčević, Snežana
AU  - Romić, Snježana Đ.
AU  - Korićanac, Goran
AU  - Živković, Maja
AU  - Stanković, Aleksandra
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5741
AB  - Background: Heart disease or diabetes could be promoted in people who consume sugar. High dietary
intake of fructose also increases risk for heart disease. The effects of fructose diet on metabolism, lipid
profile and blood pressure are dependent of sex. Renin-angiotensin system (RAS) impairs insulin sensi-
tivity, whereas hyperinsulinemia and insulin resistance promotes the development of cardiovascular dis-
orders. We examined the effects of fructose rich diet (FRD) and estradiol (E2) on the expression of ACE,
ACE2, AT1R and AT2R in the rat heart.
Methods and Results: 21 day old female rats were divided in control group and FRD group. At two
weeks before sacrifice, animals were ovariectomised and half of the FRD rats were subjected to E2 re-
placement. FRD increased ACE (p<0.001) and AT1R (p<0.05) protein expression, while AT2R expression
was decreased (p<0.05). ACE2 protein expression was unaltered. mRNA expression for AT1R was unal-
tered, as assessed by qPCR. E2 treatment significantly decreased protein expression for ACE (p<0.001)
and AT1R (p<0.01), while ACE2 (p<0.01) and AT2R (p<0.01) expression was increased. Effect of E2 treat-
ment on AT1R gene expression was not statistically significant but showed trend toward lowering AT1R
gene expression. We didn’t detected AT2R gene expression.
Conclusion: FRD affects almost all components of the RAS in the rat heart.The increase of the ACE and
AT1R protein expression and decrease of AT2R expression could be the the way that leads to the devel-
opment of cardiovascular disorders. The E2 shows strong protective effect in FRD through reversion of the
changes in RAS components expression.
C3  - Cardiology
T1  - The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol
VL  - 126
IS  - Suppl. 2
SP  - 353
EP  - 353
DO  - 10.1159/000355890
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5741
ER  - 

@conference{
author = "Bundalo, Maja M. and Tepavčević, Snežana and Romić, Snježana Đ. and Korićanac, Goran and Živković, Maja and Stanković, Aleksandra",
year = "2013",
abstract = "Background: Heart disease or diabetes could be promoted in people who consume sugar. High dietary
intake of fructose also increases risk for heart disease. The effects of fructose diet on metabolism, lipid
profile and blood pressure are dependent of sex. Renin-angiotensin system (RAS) impairs insulin sensi-
tivity, whereas hyperinsulinemia and insulin resistance promotes the development of cardiovascular dis-
orders. We examined the effects of fructose rich diet (FRD) and estradiol (E2) on the expression of ACE,
ACE2, AT1R and AT2R in the rat heart.
Methods and Results: 21 day old female rats were divided in control group and FRD group. At two
weeks before sacrifice, animals were ovariectomised and half of the FRD rats were subjected to E2 re-
placement. FRD increased ACE (p<0.001) and AT1R (p<0.05) protein expression, while AT2R expression
was decreased (p<0.05). ACE2 protein expression was unaltered. mRNA expression for AT1R was unal-
tered, as assessed by qPCR. E2 treatment significantly decreased protein expression for ACE (p<0.001)
and AT1R (p<0.01), while ACE2 (p<0.01) and AT2R (p<0.01) expression was increased. Effect of E2 treat-
ment on AT1R gene expression was not statistically significant but showed trend toward lowering AT1R
gene expression. We didn’t detected AT2R gene expression.
Conclusion: FRD affects almost all components of the RAS in the rat heart.The increase of the ACE and
AT1R protein expression and decrease of AT2R expression could be the the way that leads to the devel-
opment of cardiovascular disorders. The E2 shows strong protective effect in FRD through reversion of the
changes in RAS components expression.",
journal = "Cardiology",
title = "The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol",
volume = "126",
number = "Suppl. 2",
pages = "353-353",
doi = "10.1159/000355890",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5741"
}

Bundalo, M. M., Tepavčević, S., Romić, S. Đ., Korićanac, G., Živković, M.,& Stanković, A.. (2013). The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol. in Cardiology, 126(Suppl. 2), 353-353.
https://doi.org/10.1159/000355890
https://hdl.handle.net/21.15107/rcub_vinar_5741

Bundalo MM, Tepavčević S, Romić SĐ, Korićanac G, Živković M, Stanković A. The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol. in Cardiology. 2013;126(Suppl. 2):353-353.
doi:10.1159/000355890
https://hdl.handle.net/21.15107/rcub_vinar_5741 .

Bundalo, Maja M., Tepavčević, Snežana, Romić, Snježana Đ., Korićanac, Goran, Živković, Maja, Stanković, Aleksandra, "The expression of ACE, ACE2, AT1 and AT2 receptors in the heart of fructose fed ovariectomized rats-Effect of estradiol" in Cardiology, 126, no. Suppl. 2 (2013):353-353,
https://doi.org/10.1159/000355890 .,
https://hdl.handle.net/21.15107/rcub_vinar_5741 .