TY  - CONF
AU  - Vasović, Dolika V.
AU  - Ivković, Sanja
AU  - Jovanović Macura, Irena
AU  - Major, Tamara
AU  - Živanović, Ana
AU  - Milašin, Jelena
AU  - Nikolić, Nađa
AU  - Simonović, Jelena
AU  - Šutulović, Nikola
AU  - Hrnčić, Dragan
AU  - Stanojlović, Olivera
AU  - Mladenović, Dušan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11056
AB  - Introduction: Diabetic retinopathy (DR) is not cured efficiently and changes of lifestyle measures may alleviate its course. The aim of our study was to investigate the effects of shortened daily photoperiod on inflammation and visual cycle in rat retina and retinal pigment epithelium (RPE) in DR. Methodology: Male Wistar rats were divided into the following groups: 1. control; 2. diabetic group (DM) treated with a single intraperitoneal injection of streptozotocin (100 mg/kg); 3. group exposed to light/dark cycle 6/18h for 3 months (6/18); 4. diabetic group exposed to light/dark cycle 6/18h (DM+6/18). Retinal blood vessel permeability was estimated immunohistochemically based on lectin staining, while the expression of genes involved in the visual cycle (SOX9, LRAT, RPE65, OTX2), and inflammation (IL-1, TNF-α) was determined by qRT-PCR in the retina and RPE. Results: Shortened photoperiod reduced neovascularisation and the expression of IL-1 and TNF-α in both retina and RPE. The expression of IL-1 and TNF-α genes in the retina was significantly higher in DM vs. control group (P=0.001). In contrast, retinal IL-1 and TNF-α expressions were significantly lower in DM+6/18 vs. DM group (P=0.001). The expression of IL-1 and TNF-α in RPE was significantly higher in DM vs. control group, however the expression of these genes was significantly lower in DM+6/18 vs. DM group (PIL-1=0.008 and PTNF-α=0.002). The expression of visual cycle genes was significantly up-regulated in RPE in DM+6/18 vs. DM group (P=0.001). Conclusion: Shortened daily photoperiod reduces blood vessel permeability in DR via its anti-inflammatory effect associated with accelerated visual cycle in the retina.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats
SP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11056
ER  - 

@conference{
author = "Vasović, Dolika V. and Ivković, Sanja and Jovanović Macura, Irena and Major, Tamara and Živanović, Ana and Milašin, Jelena and Nikolić, Nađa and Simonović, Jelena and Šutulović, Nikola and Hrnčić, Dragan and Stanojlović, Olivera and Mladenović, Dušan",
year = "2023",
abstract = "Introduction: Diabetic retinopathy (DR) is not cured efficiently and changes of lifestyle measures may alleviate its course. The aim of our study was to investigate the effects of shortened daily photoperiod on inflammation and visual cycle in rat retina and retinal pigment epithelium (RPE) in DR. Methodology: Male Wistar rats were divided into the following groups: 1. control; 2. diabetic group (DM) treated with a single intraperitoneal injection of streptozotocin (100 mg/kg); 3. group exposed to light/dark cycle 6/18h for 3 months (6/18); 4. diabetic group exposed to light/dark cycle 6/18h (DM+6/18). Retinal blood vessel permeability was estimated immunohistochemically based on lectin staining, while the expression of genes involved in the visual cycle (SOX9, LRAT, RPE65, OTX2), and inflammation (IL-1, TNF-α) was determined by qRT-PCR in the retina and RPE. Results: Shortened photoperiod reduced neovascularisation and the expression of IL-1 and TNF-α in both retina and RPE. The expression of IL-1 and TNF-α genes in the retina was significantly higher in DM vs. control group (P=0.001). In contrast, retinal IL-1 and TNF-α expressions were significantly lower in DM+6/18 vs. DM group (P=0.001). The expression of IL-1 and TNF-α in RPE was significantly higher in DM vs. control group, however the expression of these genes was significantly lower in DM+6/18 vs. DM group (PIL-1=0.008 and PTNF-α=0.002). The expression of visual cycle genes was significantly up-regulated in RPE in DM+6/18 vs. DM group (P=0.001). Conclusion: Shortened daily photoperiod reduces blood vessel permeability in DR via its anti-inflammatory effect associated with accelerated visual cycle in the retina.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats",
pages = "112",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11056"
}

Vasović, D. V., Ivković, S., Jovanović Macura, I., Major, T., Živanović, A., Milašin, J., Nikolić, N., Simonović, J., Šutulović, N., Hrnčić, D., Stanojlović, O.,& Mladenović, D.. (2023). The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 112.
https://hdl.handle.net/21.15107/rcub_vinar_11056

Vasović DV, Ivković S, Jovanović Macura I, Major T, Živanović A, Milašin J, Nikolić N, Simonović J, Šutulović N, Hrnčić D, Stanojlović O, Mladenović D. The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:112.
https://hdl.handle.net/21.15107/rcub_vinar_11056 .

Vasović, Dolika V., Ivković, Sanja, Jovanović Macura, Irena, Major, Tamara, Živanović, Ana, Milašin, Jelena, Nikolić, Nađa, Simonović, Jelena, Šutulović, Nikola, Hrnčić, Dragan, Stanojlović, Olivera, Mladenović, Dušan, "The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):112,
https://hdl.handle.net/21.15107/rcub_vinar_11056 .