TY  - JOUR
AU  - Živković, Maja
AU  - Kolić, Ivana
AU  - Ješić, Snežana
AU  - Jotić, Ana
AU  - Stanković, Aleksandra
PY  - 2018
UR  - http://e-ceo.org/journal/view.php?doi=10.21053/ceo.2017.01060
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7677
AB  - Objectives. Chronic otitis media (COM) is followed by irreversible tissue damage and destruction of the middle ear structures, with the possibility of complications under the maintenance of inflammation. Inflammatory mediators such as cytokines play a crucial role in the initial stage of inflammation. The aim of this study was to evaluate the association of the polymorphisms in two innate immunity/inflammation cascade genes from interleukin-1 (IL-1) gene cluster with COM with regard to cholesteatoma. Methods. In the cross-sectional case-control study, DNA samples were collected from 189 patients with COM and 119 controls from a population of Serbia. The +3953 C/T (rs1143634), TaqI polymorphism in interleukin-1 beta (IL-1Β) gene and 86 bp variable number tandem repeat (VNTR, rs2234663) polymorphism in the IL-1 receptor antagonist (IL-1RA) gene were analyzed by polymerase chain reaction. Results. The IL-1Β TaqI polymorphism was not significantly different in patients compared with the control group. The significant difference between patients and controls was observed for both, genotype and allele frequencies of IL-1RA VNTR polymorphism (chi-square P<0.01). We found that carriers of IL-1RA allele 2 (odds ratio, 0.47; 95% confidence interval, 0.29 to 0.76; P=0.004) have a favorable association with COM, using multivariate logistic analysis that included both polymorphisms, age and sex. The IL-1RA allele frequency distribution was significantly different with regard to cholesteatoma. Conclusion. The carriers of allele 2 of VNTR IL-1RA polymorphism had a decreased odds ratio for COM, which is in agreement with findings in other inflammatory disease and its previous association with higher IL-1RA levels. Possible down-regulation of IL-1 mediated proinflammatory signaling pathways via IL-1RA in COM as well as results of our study should be further investigated and replicated.
T2  - Clinical and Experimental Otorhinolaryngology
T1  - The Allele 2 of the VNTR Polymorphism in the Gene That Encodes a Natural Inhibitor of IL-1β, IL-1RA Is Favorably Associated With Chronic Otitis Media
VL  - 11
IS  - 2
SP  - 118
EP  - 123
DO  - 10.21053/ceo.2017.01060
ER  - 

@article{
author = "Živković, Maja and Kolić, Ivana and Ješić, Snežana and Jotić, Ana and Stanković, Aleksandra",
year = "2018",
abstract = "Objectives. Chronic otitis media (COM) is followed by irreversible tissue damage and destruction of the middle ear structures, with the possibility of complications under the maintenance of inflammation. Inflammatory mediators such as cytokines play a crucial role in the initial stage of inflammation. The aim of this study was to evaluate the association of the polymorphisms in two innate immunity/inflammation cascade genes from interleukin-1 (IL-1) gene cluster with COM with regard to cholesteatoma. Methods. In the cross-sectional case-control study, DNA samples were collected from 189 patients with COM and 119 controls from a population of Serbia. The +3953 C/T (rs1143634), TaqI polymorphism in interleukin-1 beta (IL-1Β) gene and 86 bp variable number tandem repeat (VNTR, rs2234663) polymorphism in the IL-1 receptor antagonist (IL-1RA) gene were analyzed by polymerase chain reaction. Results. The IL-1Β TaqI polymorphism was not significantly different in patients compared with the control group. The significant difference between patients and controls was observed for both, genotype and allele frequencies of IL-1RA VNTR polymorphism (chi-square P<0.01). We found that carriers of IL-1RA allele 2 (odds ratio, 0.47; 95% confidence interval, 0.29 to 0.76; P=0.004) have a favorable association with COM, using multivariate logistic analysis that included both polymorphisms, age and sex. The IL-1RA allele frequency distribution was significantly different with regard to cholesteatoma. Conclusion. The carriers of allele 2 of VNTR IL-1RA polymorphism had a decreased odds ratio for COM, which is in agreement with findings in other inflammatory disease and its previous association with higher IL-1RA levels. Possible down-regulation of IL-1 mediated proinflammatory signaling pathways via IL-1RA in COM as well as results of our study should be further investigated and replicated.",
journal = "Clinical and Experimental Otorhinolaryngology",
title = "The Allele 2 of the VNTR Polymorphism in the Gene That Encodes a Natural Inhibitor of IL-1β, IL-1RA Is Favorably Associated With Chronic Otitis Media",
volume = "11",
number = "2",
pages = "118-123",
doi = "10.21053/ceo.2017.01060"
}

Živković, M., Kolić, I., Ješić, S., Jotić, A.,& Stanković, A.. (2018). The Allele 2 of the VNTR Polymorphism in the Gene That Encodes a Natural Inhibitor of IL-1β, IL-1RA Is Favorably Associated With Chronic Otitis Media. in Clinical and Experimental Otorhinolaryngology, 11(2), 118-123.
https://doi.org/10.21053/ceo.2017.01060

Živković M, Kolić I, Ješić S, Jotić A, Stanković A. The Allele 2 of the VNTR Polymorphism in the Gene That Encodes a Natural Inhibitor of IL-1β, IL-1RA Is Favorably Associated With Chronic Otitis Media. in Clinical and Experimental Otorhinolaryngology. 2018;11(2):118-123.
doi:10.21053/ceo.2017.01060 .

Živković, Maja, Kolić, Ivana, Ješić, Snežana, Jotić, Ana, Stanković, Aleksandra, "The Allele 2 of the VNTR Polymorphism in the Gene That Encodes a Natural Inhibitor of IL-1β, IL-1RA Is Favorably Associated With Chronic Otitis Media" in Clinical and Experimental Otorhinolaryngology, 11, no. 2 (2018):118-123,
https://doi.org/10.21053/ceo.2017.01060 . .

TY  - JOUR
AU  - Jotić, Ana
AU  - Ješić, Snežana
AU  - Živković, Maja
AU  - Tomanović, Nada
AU  - Kuveljić, Jovana
AU  - Stanković, Aleksandra
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/679
AB  - Objective: Toll-like receptors (TLRs) have a prominent role in inducing innate immune response. It has been suggested that regulation of TLRs is involved in the pathogenesis of chronic otitis media. TLR 2 and TLR 4 polymorphisms were connected with susceptibility to acute otitis and chronic otitis with effusion. The objective of this study was to establish expression of TLR 2 and 4 on middle ear mucosa in different types of chronic suppurative otitis media (CSOM), and the influence of gene polymorphisms TLR 2 Arg753Gln and TLR 4 Thr399Ile and Asp299Gly to susceptibility to CSOM. Material and methods: Middle ear mucosa and full blood samples were obtained from 85 patients With chronic suppurative otitis media with and without cholesteatoma. Control group for mucosal TLR expression consisted of 71 samples of middle ear mucosa taken from patients with otosclerosis, and control group for DNA polymorphism consisted of 100 full blood samples in healthy subjects. DNA polymorphism detection was done with restriction fragment length polymorphism in RI PCR. Expression of TLR 2 and 4 was determined with immunohistochemical staining. Results: TLR 2 and TLR 4 expression on the middle ear mucosa was not influenced by age of the patients with chronic otitis media. Incidence of TLR 2 Arg753Gln polymorphism was significantly higher in patients with chronic otitis media, compared to control group. Significant association between TLR 2 Arg753Gln polymorphism and different types of mucosal changes in patients with chronic otitis media was established. TLR 2 and 4 expression on experimental group mucosa was significantly different compared to control group, where there was no expression (p = 0.000). Strong dependence of TLR 2 and TLR 4 expression on middle ear mucosa with different mucosal changes and immunohistochemical activity after staining was detected. Conclusion: Certain polymorphisms in TLR genes could be indicative for susceptibility to chronic otitis media. Expression of TLR 2 and 4 on middle ear mucosa was more dependable on different types of mucosal changes and type of CSOM than on bacteria found in the specimens. This can indicate that the type of mucosal changes are closely correlated with TLRs activity in middle ear. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Auris Nasus Larynx
T1  - Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media
VL  - 42
IS  - 6
SP  - 431
EP  - 437
DO  - 10.1016/j.anl.2015.04.010
ER  - 

@article{
author = "Jotić, Ana and Ješić, Snežana and Živković, Maja and Tomanović, Nada and Kuveljić, Jovana and Stanković, Aleksandra",
year = "2015",
abstract = "Objective: Toll-like receptors (TLRs) have a prominent role in inducing innate immune response. It has been suggested that regulation of TLRs is involved in the pathogenesis of chronic otitis media. TLR 2 and TLR 4 polymorphisms were connected with susceptibility to acute otitis and chronic otitis with effusion. The objective of this study was to establish expression of TLR 2 and 4 on middle ear mucosa in different types of chronic suppurative otitis media (CSOM), and the influence of gene polymorphisms TLR 2 Arg753Gln and TLR 4 Thr399Ile and Asp299Gly to susceptibility to CSOM. Material and methods: Middle ear mucosa and full blood samples were obtained from 85 patients With chronic suppurative otitis media with and without cholesteatoma. Control group for mucosal TLR expression consisted of 71 samples of middle ear mucosa taken from patients with otosclerosis, and control group for DNA polymorphism consisted of 100 full blood samples in healthy subjects. DNA polymorphism detection was done with restriction fragment length polymorphism in RI PCR. Expression of TLR 2 and 4 was determined with immunohistochemical staining. Results: TLR 2 and TLR 4 expression on the middle ear mucosa was not influenced by age of the patients with chronic otitis media. Incidence of TLR 2 Arg753Gln polymorphism was significantly higher in patients with chronic otitis media, compared to control group. Significant association between TLR 2 Arg753Gln polymorphism and different types of mucosal changes in patients with chronic otitis media was established. TLR 2 and 4 expression on experimental group mucosa was significantly different compared to control group, where there was no expression (p = 0.000). Strong dependence of TLR 2 and TLR 4 expression on middle ear mucosa with different mucosal changes and immunohistochemical activity after staining was detected. Conclusion: Certain polymorphisms in TLR genes could be indicative for susceptibility to chronic otitis media. Expression of TLR 2 and 4 on middle ear mucosa was more dependable on different types of mucosal changes and type of CSOM than on bacteria found in the specimens. This can indicate that the type of mucosal changes are closely correlated with TLRs activity in middle ear. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Auris Nasus Larynx",
title = "Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media",
volume = "42",
number = "6",
pages = "431-437",
doi = "10.1016/j.anl.2015.04.010"
}

Jotić, A., Ješić, S., Živković, M., Tomanović, N., Kuveljić, J.,& Stanković, A.. (2015). Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media. in Auris Nasus Larynx, 42(6), 431-437.
https://doi.org/10.1016/j.anl.2015.04.010

Jotić A, Ješić S, Živković M, Tomanović N, Kuveljić J, Stanković A. Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media. in Auris Nasus Larynx. 2015;42(6):431-437.
doi:10.1016/j.anl.2015.04.010 .

Jotić, Ana, Ješić, Snežana, Živković, Maja, Tomanović, Nada, Kuveljić, Jovana, Stanković, Aleksandra, "Polymorphisms in Toll-like receptors 2 and 4 genes and their expression in chronic suppurative otitis media" in Auris Nasus Larynx, 42, no. 6 (2015):431-437,
https://doi.org/10.1016/j.anl.2015.04.010 . .

TY  - JOUR
AU  - Ješić, Snežana
AU  - Jotić, Ana
AU  - Tomanović, Nada
AU  - Živković, Maja
AU  - Kolaković, Ana
AU  - Stanković, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6051
AB  - Objectives: The objectives were to detect and compare the expression of toll-like receptors (TLRs) 2, 4 and nuclear factor kappa B in mucosal lesions of chronic otitis. Methods: Fifty-five tissue samples obtained from children and adults operated on for otitis were investigated by semiquantitative immunohistochemical methods using polyclonal antibodies for TLR 2, 4 and NFkB. Kruskal-Wallis, Mann-Whitney, and Kendalls tau rank correlation tests were used. Results: Stronger expression of TLR2, 4 was found in inflamed mucosa than in the control for children and adults (TLR2: H = 23.86, P LT .001; TLR4: H = 22.80, P LT .001) (TLR2: H = 17.53, P LT .001; TLR4: H = 11.99, P LT .001); in cholesteatoma perimatrix compared to tubotympanic lesions in children (TLR2: H = 11.06, P = .004; TLR4: H = 10.61, P = .005) and adults (TLR2: H = 10.73, P =.013; TLR4: H = 9.65, P = .021). No differences were found in NFkB expression (H = 0.042, P = .99). Significant correlations were found for all pairs of molecules in cholesteatoma and tubotympanic mucosa of adults (TLR2, 4: P = .002, P LT .001; TLR2-NfkB: P = .032, P = .021; TLR4-NFkB: P =.035, P = .0013), only TLR4-NFkB in tubotympanic otitis of children (P = .026). Conclusions: Toll-like receptors 2, 4 and NFkB mediate inflammation in cholesteatoma and mucosal lesions of tubotympanic otitis in children and adults. Significant correlations between all pairs of molecules in all samples were detected in adults, but only TLR4-NFkB in children.
T2  - Annals of Otology Rhinology and Laryngology
T1  - Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study
VL  - 123
IS  - 6
SP  - 434
EP  - 441
DO  - 10.1177/0003489414527229
ER  - 

@article{
author = "Ješić, Snežana and Jotić, Ana and Tomanović, Nada and Živković, Maja and Kolaković, Ana and Stanković, Aleksandra",
year = "2014",
abstract = "Objectives: The objectives were to detect and compare the expression of toll-like receptors (TLRs) 2, 4 and nuclear factor kappa B in mucosal lesions of chronic otitis. Methods: Fifty-five tissue samples obtained from children and adults operated on for otitis were investigated by semiquantitative immunohistochemical methods using polyclonal antibodies for TLR 2, 4 and NFkB. Kruskal-Wallis, Mann-Whitney, and Kendalls tau rank correlation tests were used. Results: Stronger expression of TLR2, 4 was found in inflamed mucosa than in the control for children and adults (TLR2: H = 23.86, P LT .001; TLR4: H = 22.80, P LT .001) (TLR2: H = 17.53, P LT .001; TLR4: H = 11.99, P LT .001); in cholesteatoma perimatrix compared to tubotympanic lesions in children (TLR2: H = 11.06, P = .004; TLR4: H = 10.61, P = .005) and adults (TLR2: H = 10.73, P =.013; TLR4: H = 9.65, P = .021). No differences were found in NFkB expression (H = 0.042, P = .99). Significant correlations were found for all pairs of molecules in cholesteatoma and tubotympanic mucosa of adults (TLR2, 4: P = .002, P LT .001; TLR2-NfkB: P = .032, P = .021; TLR4-NFkB: P =.035, P = .0013), only TLR4-NFkB in tubotympanic otitis of children (P = .026). Conclusions: Toll-like receptors 2, 4 and NFkB mediate inflammation in cholesteatoma and mucosal lesions of tubotympanic otitis in children and adults. Significant correlations between all pairs of molecules in all samples were detected in adults, but only TLR4-NFkB in children.",
journal = "Annals of Otology Rhinology and Laryngology",
title = "Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study",
volume = "123",
number = "6",
pages = "434-441",
doi = "10.1177/0003489414527229"
}

Ješić, S., Jotić, A., Tomanović, N., Živković, M., Kolaković, A.,& Stanković, A.. (2014). Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study. in Annals of Otology Rhinology and Laryngology, 123(6), 434-441.
https://doi.org/10.1177/0003489414527229

Ješić S, Jotić A, Tomanović N, Živković M, Kolaković A, Stanković A. Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study. in Annals of Otology Rhinology and Laryngology. 2014;123(6):434-441.
doi:10.1177/0003489414527229 .

Ješić, Snežana, Jotić, Ana, Tomanović, Nada, Živković, Maja, Kolaković, Ana, Stanković, Aleksandra, "Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study" in Annals of Otology Rhinology and Laryngology, 123, no. 6 (2014):434-441,
https://doi.org/10.1177/0003489414527229 . .

TY  - CONF
AU  - Jotić, Ana
AU  - Kuveljić, Jovana
AU  - Kolaković, Ana
AU  - Kolić, Ivana
AU  - Živković, Maja
AU  - Jesić, Snežana
AU  - Stanković, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12387
AB  - Toll like receptors (TLRs) are the pattern recognition receptors, important for the innate immune system, the first line of defense against bacterial infections which are the main cause of otitis media (OM). Our aim was to investigate the association of TLR4 (Thr399Ile and Asp299Gly) and TLR2 (Arg753Gln) most common polymorphisms with OM prevalence as well as with morphologic changes of middle ear during chronic otitis media. We had 111 controls and 186 patients divided into three groups of COM: nonsuppurative OM, suppurative OM and cholesteatoma. Frequencies of the genotypes of the TLR 2 gene polymorphism were significantly different between men and women having nonsuppurative OM (p=0.03).
PB  - Belgrade : Serbian Genetic Society
C3  - V Congress of the Serbian Genetic Society : Book of abstracts
T1  - Toll like receptors 2 and 4 polymorphisms in chronic otitis media
SP  - 52
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12387
ER  - 

@conference{
author = "Jotić, Ana and Kuveljić, Jovana and Kolaković, Ana and Kolić, Ivana and Živković, Maja and Jesić, Snežana and Stanković, Aleksandra",
year = "2014",
abstract = "Toll like receptors (TLRs) are the pattern recognition receptors, important for the innate immune system, the first line of defense against bacterial infections which are the main cause of otitis media (OM). Our aim was to investigate the association of TLR4 (Thr399Ile and Asp299Gly) and TLR2 (Arg753Gln) most common polymorphisms with OM prevalence as well as with morphologic changes of middle ear during chronic otitis media. We had 111 controls and 186 patients divided into three groups of COM: nonsuppurative OM, suppurative OM and cholesteatoma. Frequencies of the genotypes of the TLR 2 gene polymorphism were significantly different between men and women having nonsuppurative OM (p=0.03).",
publisher = "Belgrade : Serbian Genetic Society",
journal = "V Congress of the Serbian Genetic Society : Book of abstracts",
title = "Toll like receptors 2 and 4 polymorphisms in chronic otitis media",
pages = "52-52",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12387"
}

Jotić, A., Kuveljić, J., Kolaković, A., Kolić, I., Živković, M., Jesić, S.,& Stanković, A.. (2014). Toll like receptors 2 and 4 polymorphisms in chronic otitis media. in V Congress of the Serbian Genetic Society : Book of abstracts
Belgrade : Serbian Genetic Society., 52-52.
https://hdl.handle.net/21.15107/rcub_vinar_12387

Jotić A, Kuveljić J, Kolaković A, Kolić I, Živković M, Jesić S, Stanković A. Toll like receptors 2 and 4 polymorphisms in chronic otitis media. in V Congress of the Serbian Genetic Society : Book of abstracts. 2014;:52-52.
https://hdl.handle.net/21.15107/rcub_vinar_12387 .

Jotić, Ana, Kuveljić, Jovana, Kolaković, Ana, Kolić, Ivana, Živković, Maja, Jesić, Snežana, Stanković, Aleksandra, "Toll like receptors 2 and 4 polymorphisms in chronic otitis media" in V Congress of the Serbian Genetic Society : Book of abstracts (2014):52-52,
https://hdl.handle.net/21.15107/rcub_vinar_12387 .