TY  - JOUR
AU  - Dorontić, Slađana
AU  - Bonasera, Aurelio
AU  - Scopelliti, Michelangelo
AU  - Marković, Olivera
AU  - Verbić, Tatjana
AU  - Sredojević, Dušan
AU  - Ciasca, Gabriele
AU  - Di Santo, Riccardo
AU  - Mead, James L.
AU  - Budimir, Milica
AU  - Bajuk-Bogdanović, Danica
AU  - Mojsin, Marija
AU  - Pejić, Jelena
AU  - Stevanović, Milena
AU  - Jovanović, Svetlana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13322
AB  - The widespread usage of the herbicide glyphosate calls for urgent action, aiming at the development of new, simple, low-cost, and eco-friendly detection approaches. In the last decade, investigation of graphene quantum dots (GQDs) as potential optical probes for various pollutants rapidly grew, thanks to their easy-manipulative structure, remarkable photoluminescence (PL) in the visible part of the spectrum, good dispersibility, biocompatibility, and non-toxicity, as well. Herein, a fast, simple, and environmentally friendly method for GQDs structural modification is presented. GQDs raw powder was exposed to γ- rays at three different doses (100, 200, and 300 kGy) in air, without any solvent or reagents. Irradiation of dots under such affordable conditions led to the additional incorporation of oxygen-containing moieties in the GQD structure. For the first time, oxygen-rich GQDs irradiated at a 300 kGy dose were successfully applied as direct turn-off PL probe for glyphosate detection. The high coefficient of determination (R-squared (R2) = 0.99) and very low limit of detection (3.02 μmol L-1) reveal good linearity between the potential sensor and analyte, as well as sensitivity. Glyphosate was successfully detected in celery samples, with a recovery value of 107 ± 0.85%. To evaluate the biological safety of the proposed sensing probe, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) and the hemolysis assays were performed. Obtained results show that irradiated and non-irradiated GQDs did not cause the death of MRC-5 cells, and hemolysis of erythrocytes. The obtained results demonstrate that GQDs irradiated in an air medium can be potentially applied for glyphosate detection.
T2  - Journal of Environmental Chemical Engineering
T1  - High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights
VL  - 12
IS  - 4
SP  - 113193
DO  - 10.1016/j.jece.2024.113193
ER  - 

@article{
author = "Dorontić, Slađana and Bonasera, Aurelio and Scopelliti, Michelangelo and Marković, Olivera and Verbić, Tatjana and Sredojević, Dušan and Ciasca, Gabriele and Di Santo, Riccardo and Mead, James L. and Budimir, Milica and Bajuk-Bogdanović, Danica and Mojsin, Marija and Pejić, Jelena and Stevanović, Milena and Jovanović, Svetlana",
year = "2024",
abstract = "The widespread usage of the herbicide glyphosate calls for urgent action, aiming at the development of new, simple, low-cost, and eco-friendly detection approaches. In the last decade, investigation of graphene quantum dots (GQDs) as potential optical probes for various pollutants rapidly grew, thanks to their easy-manipulative structure, remarkable photoluminescence (PL) in the visible part of the spectrum, good dispersibility, biocompatibility, and non-toxicity, as well. Herein, a fast, simple, and environmentally friendly method for GQDs structural modification is presented. GQDs raw powder was exposed to γ- rays at three different doses (100, 200, and 300 kGy) in air, without any solvent or reagents. Irradiation of dots under such affordable conditions led to the additional incorporation of oxygen-containing moieties in the GQD structure. For the first time, oxygen-rich GQDs irradiated at a 300 kGy dose were successfully applied as direct turn-off PL probe for glyphosate detection. The high coefficient of determination (R-squared (R2) = 0.99) and very low limit of detection (3.02 μmol L-1) reveal good linearity between the potential sensor and analyte, as well as sensitivity. Glyphosate was successfully detected in celery samples, with a recovery value of 107 ± 0.85%. To evaluate the biological safety of the proposed sensing probe, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) and the hemolysis assays were performed. Obtained results show that irradiated and non-irradiated GQDs did not cause the death of MRC-5 cells, and hemolysis of erythrocytes. The obtained results demonstrate that GQDs irradiated in an air medium can be potentially applied for glyphosate detection.",
journal = "Journal of Environmental Chemical Engineering",
title = "High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights",
volume = "12",
number = "4",
pages = "113193",
doi = "10.1016/j.jece.2024.113193"
}

Dorontić, S., Bonasera, A., Scopelliti, M., Marković, O., Verbić, T., Sredojević, D., Ciasca, G., Di Santo, R., Mead, J. L., Budimir, M., Bajuk-Bogdanović, D., Mojsin, M., Pejić, J., Stevanović, M.,& Jovanović, S.. (2024). High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights. in Journal of Environmental Chemical Engineering, 12(4), 113193.
https://doi.org/10.1016/j.jece.2024.113193

Dorontić S, Bonasera A, Scopelliti M, Marković O, Verbić T, Sredojević D, Ciasca G, Di Santo R, Mead JL, Budimir M, Bajuk-Bogdanović D, Mojsin M, Pejić J, Stevanović M, Jovanović S. High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights. in Journal of Environmental Chemical Engineering. 2024;12(4):113193.
doi:10.1016/j.jece.2024.113193 .

Dorontić, Slađana, Bonasera, Aurelio, Scopelliti, Michelangelo, Marković, Olivera, Verbić, Tatjana, Sredojević, Dušan, Ciasca, Gabriele, Di Santo, Riccardo, Mead, James L., Budimir, Milica, Bajuk-Bogdanović, Danica, Mojsin, Marija, Pejić, Jelena, Stevanović, Milena, Jovanović, Svetlana, "High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights" in Journal of Environmental Chemical Engineering, 12, no. 4 (2024):113193,
https://doi.org/10.1016/j.jece.2024.113193 . .

TY  - JOUR
AU  - Milenković, Mila
AU  - Mišović, Aleksandra
AU  - Jovanović, Dragana J.
AU  - Popović-Bijelić, Ana D.
AU  - Ciasca, Gabriele
AU  - Romanò, Sabrina
AU  - Bonasera, Aurelio
AU  - Mojsin, Marija
AU  - Pejić, Jelena
AU  - Stevanović, Milena J.
AU  - Jovanović, Svetlana P.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9873
AB  - Nowadays, a larger number of aggressive and corrosive chemical reagents as well as toxic solvents are used to achieve structural modification and cleaning of the final products. These lead to the production of residual, waste chemicals, which are often reactive, cancerogenic, and toxic to the environment. This study shows a new approach to the modification of graphene quantum dots (GQDs) using gamma irradiation where the usage of reagents was avoided. We achieved the incorporation of S and N atoms in the GQD structure by selecting an aqueous solution of L-cysteine as an irradiation medium. GQDs were exposed to gamma-irradiation at doses of 25, 50 and 200 kGy. After irradiation, the optical, structural, and morphological properties, as well as the possibility of their use as an agent in bioimaging and photodynamic therapy, were studied. We measured an enhanced quantum yield of photoluminescence with the highest dose of 25 kGy (21.60%). Both S- and N-functional groups were detected in all gamma-irradiated GQDs: amino, amide, thiol, and thione. Spin trap electron paramagnetic resonance showed that GQDs irradiated with 25 kGy can generate singlet oxygen upon illumination. Bioimaging on HeLa cells showed the best visibility for cells treated with GQDs irradiated with 25 kGy, while cytotoxicity was not detected after treatment of HeLa cells with gamma-irradiated GQDs.
T2  - Nanomaterials
T1  - Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent
VL  - 11
IS  - 8
SP  - 1879
DO  - 10.3390/nano11081879
ER  - 

@article{
author = "Milenković, Mila and Mišović, Aleksandra and Jovanović, Dragana J. and Popović-Bijelić, Ana D. and Ciasca, Gabriele and Romanò, Sabrina and Bonasera, Aurelio and Mojsin, Marija and Pejić, Jelena and Stevanović, Milena J. and Jovanović, Svetlana P.",
year = "2021",
abstract = "Nowadays, a larger number of aggressive and corrosive chemical reagents as well as toxic solvents are used to achieve structural modification and cleaning of the final products. These lead to the production of residual, waste chemicals, which are often reactive, cancerogenic, and toxic to the environment. This study shows a new approach to the modification of graphene quantum dots (GQDs) using gamma irradiation where the usage of reagents was avoided. We achieved the incorporation of S and N atoms in the GQD structure by selecting an aqueous solution of L-cysteine as an irradiation medium. GQDs were exposed to gamma-irradiation at doses of 25, 50 and 200 kGy. After irradiation, the optical, structural, and morphological properties, as well as the possibility of their use as an agent in bioimaging and photodynamic therapy, were studied. We measured an enhanced quantum yield of photoluminescence with the highest dose of 25 kGy (21.60%). Both S- and N-functional groups were detected in all gamma-irradiated GQDs: amino, amide, thiol, and thione. Spin trap electron paramagnetic resonance showed that GQDs irradiated with 25 kGy can generate singlet oxygen upon illumination. Bioimaging on HeLa cells showed the best visibility for cells treated with GQDs irradiated with 25 kGy, while cytotoxicity was not detected after treatment of HeLa cells with gamma-irradiated GQDs.",
journal = "Nanomaterials",
title = "Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent",
volume = "11",
number = "8",
pages = "1879",
doi = "10.3390/nano11081879"
}

Milenković, M., Mišović, A., Jovanović, D. J., Popović-Bijelić, A. D., Ciasca, G., Romanò, S., Bonasera, A., Mojsin, M., Pejić, J., Stevanović, M. J.,& Jovanović, S. P.. (2021). Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent. in Nanomaterials, 11(8), 1879.
https://doi.org/10.3390/nano11081879

Milenković M, Mišović A, Jovanović DJ, Popović-Bijelić AD, Ciasca G, Romanò S, Bonasera A, Mojsin M, Pejić J, Stevanović MJ, Jovanović SP. Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent. in Nanomaterials. 2021;11(8):1879.
doi:10.3390/nano11081879 .

Milenković, Mila, Mišović, Aleksandra, Jovanović, Dragana J., Popović-Bijelić, Ana D., Ciasca, Gabriele, Romanò, Sabrina, Bonasera, Aurelio, Mojsin, Marija, Pejić, Jelena, Stevanović, Milena J., Jovanović, Svetlana P., "Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent" in Nanomaterials, 11, no. 8 (2021):1879,
https://doi.org/10.3390/nano11081879 . .