TY  - JOUR
AU  - Milosavljević, Slobodan
AU  - Đorđević, Iris
AU  - Mandić, Boris
AU  - Tešević, Vele V.
AU  - Stanković, Miroslava
AU  - Todorović, Nina
AU  - Novaković, Miroslav M.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10107
AB  - In continuation of our study on Cotinus coggygria from Serbia, 10 known flavonoids (1-10) were isolated from the methylene chloride/ methanol extract of the heartwood. They were tested for in vitro protective effect against chromosome aberrations in peripheral human lymphocytes, using the cytokinesis-block micronucleus assay. All tested compounds (in minimal doses of 1 μg/mL) exerted a beneficial effect by decreasing DNA damage of human lymphocytes in the range of 24.2% to 54.5%, better than the radio protectant control, amifostine. Functional groups, such as 3′,4′-dihydroxyphenyl (catechol), 5-OH, 3-OH, and 4-keto in flavonoids (3-keto in aurones), which play a key role in antioxidant activity, are proposed to be responsible for the DNA protective activity of the tested compounds.
T2  - Natural Product Communications
T1  - Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA
VL  - 16
IS  - 12
SP  - 1
EP  - 8
DO  - 10.1177/1934578X211067289
ER  - 

@article{
author = "Milosavljević, Slobodan and Đorđević, Iris and Mandić, Boris and Tešević, Vele V. and Stanković, Miroslava and Todorović, Nina and Novaković, Miroslav M.",
year = "2021",
abstract = "In continuation of our study on Cotinus coggygria from Serbia, 10 known flavonoids (1-10) were isolated from the methylene chloride/ methanol extract of the heartwood. They were tested for in vitro protective effect against chromosome aberrations in peripheral human lymphocytes, using the cytokinesis-block micronucleus assay. All tested compounds (in minimal doses of 1 μg/mL) exerted a beneficial effect by decreasing DNA damage of human lymphocytes in the range of 24.2% to 54.5%, better than the radio protectant control, amifostine. Functional groups, such as 3′,4′-dihydroxyphenyl (catechol), 5-OH, 3-OH, and 4-keto in flavonoids (3-keto in aurones), which play a key role in antioxidant activity, are proposed to be responsible for the DNA protective activity of the tested compounds.",
journal = "Natural Product Communications",
title = "Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA",
volume = "16",
number = "12",
pages = "1-8",
doi = "10.1177/1934578X211067289"
}

Milosavljević, S., Đorđević, I., Mandić, B., Tešević, V. V., Stanković, M., Todorović, N.,& Novaković, M. M.. (2021). Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA. in Natural Product Communications, 16(12), 1-8.
https://doi.org/10.1177/1934578X211067289

Milosavljević S, Đorđević I, Mandić B, Tešević VV, Stanković M, Todorović N, Novaković MM. Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA. in Natural Product Communications. 2021;16(12):1-8.
doi:10.1177/1934578X211067289 .

Milosavljević, Slobodan, Đorđević, Iris, Mandić, Boris, Tešević, Vele V., Stanković, Miroslava, Todorović, Nina, Novaković, Miroslav M., "Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA" in Natural Product Communications, 16, no. 12 (2021):1-8,
https://doi.org/10.1177/1934578X211067289 . .

TY  - JOUR
AU  - Stanković, Miroslava
AU  - Tešević, Vele V.
AU  - Vajs, Vlatka
AU  - Todorović, Nina
AU  - Milosavljević, Slobodan
AU  - Godevac, Dejan
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3481
AB  - The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and (13)C-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyte damage by ROS.
T2  - Planta Medica
T1  - Antioxidant properties of grape seed extract on human lymphocyte oxidative defence
VL  - 74
IS  - 7
SP  - 730
EP  - 735
DO  - 10.1055/s-2008-1074521
ER  - 

@article{
author = "Stanković, Miroslava and Tešević, Vele V. and Vajs, Vlatka and Todorović, Nina and Milosavljević, Slobodan and Godevac, Dejan",
year = "2008",
abstract = "The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and (13)C-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyte damage by ROS.",
journal = "Planta Medica",
title = "Antioxidant properties of grape seed extract on human lymphocyte oxidative defence",
volume = "74",
number = "7",
pages = "730-735",
doi = "10.1055/s-2008-1074521"
}

Stanković, M., Tešević, V. V., Vajs, V., Todorović, N., Milosavljević, S.,& Godevac, D.. (2008). Antioxidant properties of grape seed extract on human lymphocyte oxidative defence. in Planta Medica, 74(7), 730-735.
https://doi.org/10.1055/s-2008-1074521

Stanković M, Tešević VV, Vajs V, Todorović N, Milosavljević S, Godevac D. Antioxidant properties of grape seed extract on human lymphocyte oxidative defence. in Planta Medica. 2008;74(7):730-735.
doi:10.1055/s-2008-1074521 .

Stanković, Miroslava, Tešević, Vele V., Vajs, Vlatka, Todorović, Nina, Milosavljević, Slobodan, Godevac, Dejan, "Antioxidant properties of grape seed extract on human lymphocyte oxidative defence" in Planta Medica, 74, no. 7 (2008):730-735,
https://doi.org/10.1055/s-2008-1074521 . .