TY  - JOUR
AU  - Jovanović, Svetlana P.
AU  - Holclajtner-Antunović, Ivanka D.
AU  - Uskoković-Marković, Snežana
AU  - Bajuk-Bogdanović, Danica V.
AU  - Pavlović, Vladimir B.
AU  - Tošić, Dragana
AU  - Milenković, Marina
AU  - Todorović-Marković, Biljana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7611
AB  - © 2018 Antibacterial properties of graphene oxide (GO) have been studied extensively in the last few years, while for polyoxometalates there are just a few researches. Herein, we prepared nanocomposites of GO and different amounts of 12-molybdophosphoric acid (MoPA) and analyzed their antibacterial activity and both structural and morphological properties. In nanocomposites with higher amounts of MoPA, graphene sheets were significantly changed with disrupted flat, graphene-like morphology of GO. In the nanocomposites with low MoPA content, flat GO morphology was preserved. Additionally, structural analyses showed some changes in symmetry of the Keggin anion as a consequence of interactions between GO and MoPA, the increase in structural disorder and the lowering of electron density in GO structure due to interaction with MoPA. We have studied antibacterial properties on the gram-positive: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis; and the gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella abony. Only a mild antibacterial effect of GO and nanocomposites with low amount of MoPA against Bacillus subtilis was observed, while the rest of analyzed materials did not show any antibacterial activity.
T2  - Materials Chemistry and Physics
T1  - Modification of graphene oxide surfaces with 12-molybdophosphoric acid: Structural and antibacterial study
VL  - 213
SP  - 157
EP  - 167
DO  - 10.1016/j.matchemphys.2018.04.011
ER  - 

@article{
author = "Jovanović, Svetlana P. and Holclajtner-Antunović, Ivanka D. and Uskoković-Marković, Snežana and Bajuk-Bogdanović, Danica V. and Pavlović, Vladimir B. and Tošić, Dragana and Milenković, Marina and Todorović-Marković, Biljana",
year = "2018",
abstract = "© 2018 Antibacterial properties of graphene oxide (GO) have been studied extensively in the last few years, while for polyoxometalates there are just a few researches. Herein, we prepared nanocomposites of GO and different amounts of 12-molybdophosphoric acid (MoPA) and analyzed their antibacterial activity and both structural and morphological properties. In nanocomposites with higher amounts of MoPA, graphene sheets were significantly changed with disrupted flat, graphene-like morphology of GO. In the nanocomposites with low MoPA content, flat GO morphology was preserved. Additionally, structural analyses showed some changes in symmetry of the Keggin anion as a consequence of interactions between GO and MoPA, the increase in structural disorder and the lowering of electron density in GO structure due to interaction with MoPA. We have studied antibacterial properties on the gram-positive: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis; and the gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella abony. Only a mild antibacterial effect of GO and nanocomposites with low amount of MoPA against Bacillus subtilis was observed, while the rest of analyzed materials did not show any antibacterial activity.",
journal = "Materials Chemistry and Physics",
title = "Modification of graphene oxide surfaces with 12-molybdophosphoric acid: Structural and antibacterial study",
volume = "213",
pages = "157-167",
doi = "10.1016/j.matchemphys.2018.04.011"
}

Jovanović, S. P., Holclajtner-Antunović, I. D., Uskoković-Marković, S., Bajuk-Bogdanović, D. V., Pavlović, V. B., Tošić, D., Milenković, M.,& Todorović-Marković, B.. (2018). Modification of graphene oxide surfaces with 12-molybdophosphoric acid: Structural and antibacterial study. in Materials Chemistry and Physics, 213, 157-167.
https://doi.org/10.1016/j.matchemphys.2018.04.011

Jovanović SP, Holclajtner-Antunović ID, Uskoković-Marković S, Bajuk-Bogdanović DV, Pavlović VB, Tošić D, Milenković M, Todorović-Marković B. Modification of graphene oxide surfaces with 12-molybdophosphoric acid: Structural and antibacterial study. in Materials Chemistry and Physics. 2018;213:157-167.
doi:10.1016/j.matchemphys.2018.04.011 .

Jovanović, Svetlana P., Holclajtner-Antunović, Ivanka D., Uskoković-Marković, Snežana, Bajuk-Bogdanović, Danica V., Pavlović, Vladimir B., Tošić, Dragana, Milenković, Marina, Todorović-Marković, Biljana, "Modification of graphene oxide surfaces with 12-molybdophosphoric acid: Structural and antibacterial study" in Materials Chemistry and Physics, 213 (2018):157-167,
https://doi.org/10.1016/j.matchemphys.2018.04.011 . .

TY  - JOUR
AU  - Tomić, Sergej
AU  - Janjetović, Kristina D.
AU  - Mihajlovic, Dusan
AU  - Milenković, Marina
AU  - Kravić-Stevović, Tamara K.
AU  - Marković, Zoran M.
AU  - Todorović-Marković, Biljana
AU  - Špitalsky, Zdenko
AU  - Mičušik, Matej
AU  - Vucevic, Dragana
AU  - Colic, Miodrag
AU  - Trajković, Vladimir S.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1782
AB  - Graphene quantum dots (GQD) are atom-thick nanodimensional carbon sheets with excellent physicochemical and biological properties; making them attractive for application in theranostics: However, their immunoregulatory properties are insufficiently investigated, especially in human primary immune cells. We found that non-toxic doses of GQD inhibit the production of proinflammatory and T helper (Th) 1 cytokines, and augment the production of anti-inflammatory and Th2 cytokines by human peripheral blood mononuclear cells. While unable to affect T cells directly, GQD impaired the differentiation and functions of monocyte-derived dendritic cells (DC), lowering their capacity to stimulate T cell proliferation, development of Thl and Th17 cells, and T-cell mediated cytotoxicity. Additionally, GQD-treated DC potentiated Th2 polarization, and induced suppressive CD4(+)CD25(high)Foxp3(+) regulatory T cells. After internalization in a dynamin-independent, cholesterol-dependent manner, GQD lowered the production of reactive oxygen species and nuclear translocation of NF-kappa B in DC. The activity of mammalian target of rapamycin (mTOR) was reduced by GQD, which correlated with the increase in transcription of autophagy genes and autophagic flux in DC. Genetic suppression of autophagy impaired the pro-tolerogenic effects of GQD on DC. Our results suggest that GQD-triggered autophagy promotes tolerogenic functions in monocyte-derived DC, which could be beneficial in inflammatory T-cell mediated pathologies, but also harmful in GQD-based anti-cancer therapy. (C) 2017 Elsevier Ltd. All rights reserved.
T2  - Biomaterials
T1  - Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells
VL  - 146
SP  - 13
EP  - 28
DO  - 10.1016/j.biomaterials.2017.08.040
ER  - 

@article{
author = "Tomić, Sergej and Janjetović, Kristina D. and Mihajlovic, Dusan and Milenković, Marina and Kravić-Stevović, Tamara K. and Marković, Zoran M. and Todorović-Marković, Biljana and Špitalsky, Zdenko and Mičušik, Matej and Vucevic, Dragana and Colic, Miodrag and Trajković, Vladimir S.",
year = "2017",
abstract = "Graphene quantum dots (GQD) are atom-thick nanodimensional carbon sheets with excellent physicochemical and biological properties; making them attractive for application in theranostics: However, their immunoregulatory properties are insufficiently investigated, especially in human primary immune cells. We found that non-toxic doses of GQD inhibit the production of proinflammatory and T helper (Th) 1 cytokines, and augment the production of anti-inflammatory and Th2 cytokines by human peripheral blood mononuclear cells. While unable to affect T cells directly, GQD impaired the differentiation and functions of monocyte-derived dendritic cells (DC), lowering their capacity to stimulate T cell proliferation, development of Thl and Th17 cells, and T-cell mediated cytotoxicity. Additionally, GQD-treated DC potentiated Th2 polarization, and induced suppressive CD4(+)CD25(high)Foxp3(+) regulatory T cells. After internalization in a dynamin-independent, cholesterol-dependent manner, GQD lowered the production of reactive oxygen species and nuclear translocation of NF-kappa B in DC. The activity of mammalian target of rapamycin (mTOR) was reduced by GQD, which correlated with the increase in transcription of autophagy genes and autophagic flux in DC. Genetic suppression of autophagy impaired the pro-tolerogenic effects of GQD on DC. Our results suggest that GQD-triggered autophagy promotes tolerogenic functions in monocyte-derived DC, which could be beneficial in inflammatory T-cell mediated pathologies, but also harmful in GQD-based anti-cancer therapy. (C) 2017 Elsevier Ltd. All rights reserved.",
journal = "Biomaterials",
title = "Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells",
volume = "146",
pages = "13-28",
doi = "10.1016/j.biomaterials.2017.08.040"
}

Tomić, S., Janjetović, K. D., Mihajlovic, D., Milenković, M., Kravić-Stevović, T. K., Marković, Z. M., Todorović-Marković, B., Špitalsky, Z., Mičušik, M., Vucevic, D., Colic, M.,& Trajković, V. S.. (2017). Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells. in Biomaterials, 146, 13-28.
https://doi.org/10.1016/j.biomaterials.2017.08.040

Tomić S, Janjetović KD, Mihajlovic D, Milenković M, Kravić-Stevović TK, Marković ZM, Todorović-Marković B, Špitalsky Z, Mičušik M, Vucevic D, Colic M, Trajković VS. Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells. in Biomaterials. 2017;146:13-28.
doi:10.1016/j.biomaterials.2017.08.040 .

Tomić, Sergej, Janjetović, Kristina D., Mihajlovic, Dusan, Milenković, Marina, Kravić-Stevović, Tamara K., Marković, Zoran M., Todorović-Marković, Biljana, Špitalsky, Zdenko, Mičušik, Matej, Vucevic, Dragana, Colic, Miodrag, Trajković, Vladimir S., "Graphene quantum dots suppress proinflammatory T cell responses via autophagy-dependent induction of tolerogenic dendritic cells" in Biomaterials, 146 (2017):13-28,
https://doi.org/10.1016/j.biomaterials.2017.08.040 . .

TY  - JOUR
AU  - Romanović, Mima Č.
AU  - Čobeljić, Božidar R.
AU  - Pevec, Andrej
AU  - Turel, Iztok
AU  - Spasojević, Vojislav
AU  - Tsaturyan, Arshak A.
AU  - Shcherbakov, Igor N.
AU  - Anđelković, Katarina
AU  - Milenković, Marina
AU  - Radanović, Dušanka
AU  - Milenković, Milica R.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1566
AB  - A dinuclear double end-on azido bridged KM complex [Ni2L2(mu-(1),(1)-N-3)(2)(N-3)(2)] (1) with the condensation product of 2-quinolinecarboxaldehyde and trimethylammonium acetohydrazide chloride (Girards T reagent) (HLCl) has been synthesized and characterized by elemental analysis, IR spectroscopy, single-crystal X-ray diffraction, magnetic measurements and DFT studies. In complex 1 intra-dimer ferromagnetic coupling between Ni2+ ions (J= +12.0(2) cm(-1)) and inter-dimer antiferromagnetic interaction (delta = -0.8(3) cm(-1)) were observed. DFT-BS calculations provided explanation of ferromagnetic exchange coupling in complex 1. (C) 2017 Elsevier Ltd. All rights reserved.
T2  - Polyhedron
T1  - Synthesis, crystal structure, magnetic properties and DFT study of dinuclear Ni(II) complex with the condensation product of 2-quinolinecarboxaldehyde and Girards T reagent
VL  - 128
SP  - 30
EP  - 37
DO  - 10.1016/j.poly.2017.02.039
ER  - 

@article{
author = "Romanović, Mima Č. and Čobeljić, Božidar R. and Pevec, Andrej and Turel, Iztok and Spasojević, Vojislav and Tsaturyan, Arshak A. and Shcherbakov, Igor N. and Anđelković, Katarina and Milenković, Marina and Radanović, Dušanka and Milenković, Milica R.",
year = "2017",
abstract = "A dinuclear double end-on azido bridged KM complex [Ni2L2(mu-(1),(1)-N-3)(2)(N-3)(2)] (1) with the condensation product of 2-quinolinecarboxaldehyde and trimethylammonium acetohydrazide chloride (Girards T reagent) (HLCl) has been synthesized and characterized by elemental analysis, IR spectroscopy, single-crystal X-ray diffraction, magnetic measurements and DFT studies. In complex 1 intra-dimer ferromagnetic coupling between Ni2+ ions (J= +12.0(2) cm(-1)) and inter-dimer antiferromagnetic interaction (delta = -0.8(3) cm(-1)) were observed. DFT-BS calculations provided explanation of ferromagnetic exchange coupling in complex 1. (C) 2017 Elsevier Ltd. All rights reserved.",
journal = "Polyhedron",
title = "Synthesis, crystal structure, magnetic properties and DFT study of dinuclear Ni(II) complex with the condensation product of 2-quinolinecarboxaldehyde and Girards T reagent",
volume = "128",
pages = "30-37",
doi = "10.1016/j.poly.2017.02.039"
}

Romanović, M. Č., Čobeljić, B. R., Pevec, A., Turel, I., Spasojević, V., Tsaturyan, A. A., Shcherbakov, I. N., Anđelković, K., Milenković, M., Radanović, D.,& Milenković, M. R.. (2017). Synthesis, crystal structure, magnetic properties and DFT study of dinuclear Ni(II) complex with the condensation product of 2-quinolinecarboxaldehyde and Girards T reagent. in Polyhedron, 128, 30-37.
https://doi.org/10.1016/j.poly.2017.02.039

Romanović MČ, Čobeljić BR, Pevec A, Turel I, Spasojević V, Tsaturyan AA, Shcherbakov IN, Anđelković K, Milenković M, Radanović D, Milenković MR. Synthesis, crystal structure, magnetic properties and DFT study of dinuclear Ni(II) complex with the condensation product of 2-quinolinecarboxaldehyde and Girards T reagent. in Polyhedron. 2017;128:30-37.
doi:10.1016/j.poly.2017.02.039 .

Romanović, Mima Č., Čobeljić, Božidar R., Pevec, Andrej, Turel, Iztok, Spasojević, Vojislav, Tsaturyan, Arshak A., Shcherbakov, Igor N., Anđelković, Katarina, Milenković, Marina, Radanović, Dušanka, Milenković, Milica R., "Synthesis, crystal structure, magnetic properties and DFT study of dinuclear Ni(II) complex with the condensation product of 2-quinolinecarboxaldehyde and Girards T reagent" in Polyhedron, 128 (2017):30-37,
https://doi.org/10.1016/j.poly.2017.02.039 . .

TY  - JOUR
AU  - Ristić, Biljana Z.
AU  - Milenković, Marina
AU  - Dakic, Ivana R.
AU  - Todorović-Marković, Biljana
AU  - Milosavljević, Momir
AU  - Budimir, Milica
AU  - Paunović, Verica G.
AU  - Dramićanin, Miroslav
AU  - Marković, Zoran M.
AU  - Trajković, Vladimir S.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5964
AB  - Synthesis of new antibacterial agents is becoming increasingly important in light of the emerging antibiotic resistance. In the present study we report that electrochemically produced graphene quantum dots (GQD), a new class of carbon nanoparticles, generate reactive oxygen species when photoexcited (470 nm, 1 W), and kill two strains of pathogenic bacteria, methicillin-resistant Staphylococcus aureus and Escherichia coli. Bacterial killing was demonstrated by the reduction in number of bacterial colonies in a standard plate count method, the increase in propidium iodide uptake confirming the cell membrane damage, as well as by morphological defects visualized by atomic force microscopy. The induction of oxidative stress in bacteria exposed to photoexcited GQD was confirmed by staining with a redox-sensitive fluorochrome dihydrorhodamine 123. Neither GQD nor light exposure alone were able to cause oxidative stress and reduce the viability of bacteria. Importantly, mouse spleen cells were markedly less sensitive in the same experimental conditions, thus indicating a fairly selective antibacterial photodynamic action of GQD. (C) 2014 Elsevier Ltd. All rights reserved.
T2  - Biomaterials
T1  - Photodynamic antibacterial effect of graphene quantum dots
VL  - 35
IS  - 15
SP  - 4428
EP  - 4435
DO  - 10.1016/j.biomaterials.2014.02.014
ER  - 

@article{
author = "Ristić, Biljana Z. and Milenković, Marina and Dakic, Ivana R. and Todorović-Marković, Biljana and Milosavljević, Momir and Budimir, Milica and Paunović, Verica G. and Dramićanin, Miroslav and Marković, Zoran M. and Trajković, Vladimir S.",
year = "2014",
abstract = "Synthesis of new antibacterial agents is becoming increasingly important in light of the emerging antibiotic resistance. In the present study we report that electrochemically produced graphene quantum dots (GQD), a new class of carbon nanoparticles, generate reactive oxygen species when photoexcited (470 nm, 1 W), and kill two strains of pathogenic bacteria, methicillin-resistant Staphylococcus aureus and Escherichia coli. Bacterial killing was demonstrated by the reduction in number of bacterial colonies in a standard plate count method, the increase in propidium iodide uptake confirming the cell membrane damage, as well as by morphological defects visualized by atomic force microscopy. The induction of oxidative stress in bacteria exposed to photoexcited GQD was confirmed by staining with a redox-sensitive fluorochrome dihydrorhodamine 123. Neither GQD nor light exposure alone were able to cause oxidative stress and reduce the viability of bacteria. Importantly, mouse spleen cells were markedly less sensitive in the same experimental conditions, thus indicating a fairly selective antibacterial photodynamic action of GQD. (C) 2014 Elsevier Ltd. All rights reserved.",
journal = "Biomaterials",
title = "Photodynamic antibacterial effect of graphene quantum dots",
volume = "35",
number = "15",
pages = "4428-4435",
doi = "10.1016/j.biomaterials.2014.02.014"
}

Ristić, B. Z., Milenković, M., Dakic, I. R., Todorović-Marković, B., Milosavljević, M., Budimir, M., Paunović, V. G., Dramićanin, M., Marković, Z. M.,& Trajković, V. S.. (2014). Photodynamic antibacterial effect of graphene quantum dots. in Biomaterials, 35(15), 4428-4435.
https://doi.org/10.1016/j.biomaterials.2014.02.014

Ristić BZ, Milenković M, Dakic IR, Todorović-Marković B, Milosavljević M, Budimir M, Paunović VG, Dramićanin M, Marković ZM, Trajković VS. Photodynamic antibacterial effect of graphene quantum dots. in Biomaterials. 2014;35(15):4428-4435.
doi:10.1016/j.biomaterials.2014.02.014 .

Ristić, Biljana Z., Milenković, Marina, Dakic, Ivana R., Todorović-Marković, Biljana, Milosavljević, Momir, Budimir, Milica, Paunović, Verica G., Dramićanin, Miroslav, Marković, Zoran M., Trajković, Vladimir S., "Photodynamic antibacterial effect of graphene quantum dots" in Biomaterials, 35, no. 15 (2014):4428-4435,
https://doi.org/10.1016/j.biomaterials.2014.02.014 . .

TY  - JOUR
AU  - Milenković, Milica
AU  - Cantoni, Giulia
AU  - Bacchi, Alessia
AU  - Spasojević, Vojislav
AU  - Milenković, Marina
AU  - Sladić, Dušan M.
AU  - Krstić, Natalija
AU  - Anđelković, Katarina
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/99
AB  - Complexes of Pd(II) and Fe(III) with the condensation derivative of 2-(diphenylphosphino)benzaldehyde and ethyl carbazate were synthesized, characterized, and their antimicrobial activity was evaluated. The structures of the Pd(II) and Fe(III) complexes in solid state were determined by IR, elemental analysis and X-ray analysis. The structure of Pd(II) complex in solution was determined by NMR spectroscopy. Results of magnetic measurements for Fe(III) complex were reported. In both complexes the ligand is coordinated as tridentate via the phosphorus, the imine nitrogen and the carbonyl oxygen atoms. Results of antimicrobial activity investigation indicate that the activity of the ligand is enhanced upon complexation, and that the MIC values of the iron complex to some bacterial strains are not much higher than those of cefotaxime. (C) 2014 Elsevier Ltd. All rights reserved.
T2  - Polyhedron
T1  - Synthesis, characterization and antimicrobial activity of Pd(II) and Fe(III) complexes with ethyl (2E)-2-[2-(diphenylphosphino)benzylidene]hydrazinecarboxylate
VL  - 80
IS  - SI
SP  - 47
EP  - 52
DO  - 10.1016/j.poly.2014.01.022
ER  - 

@article{
author = "Milenković, Milica and Cantoni, Giulia and Bacchi, Alessia and Spasojević, Vojislav and Milenković, Marina and Sladić, Dušan M. and Krstić, Natalija and Anđelković, Katarina",
year = "2014",
abstract = "Complexes of Pd(II) and Fe(III) with the condensation derivative of 2-(diphenylphosphino)benzaldehyde and ethyl carbazate were synthesized, characterized, and their antimicrobial activity was evaluated. The structures of the Pd(II) and Fe(III) complexes in solid state were determined by IR, elemental analysis and X-ray analysis. The structure of Pd(II) complex in solution was determined by NMR spectroscopy. Results of magnetic measurements for Fe(III) complex were reported. In both complexes the ligand is coordinated as tridentate via the phosphorus, the imine nitrogen and the carbonyl oxygen atoms. Results of antimicrobial activity investigation indicate that the activity of the ligand is enhanced upon complexation, and that the MIC values of the iron complex to some bacterial strains are not much higher than those of cefotaxime. (C) 2014 Elsevier Ltd. All rights reserved.",
journal = "Polyhedron",
title = "Synthesis, characterization and antimicrobial activity of Pd(II) and Fe(III) complexes with ethyl (2E)-2-[2-(diphenylphosphino)benzylidene]hydrazinecarboxylate",
volume = "80",
number = "SI",
pages = "47-52",
doi = "10.1016/j.poly.2014.01.022"
}

Milenković, M., Cantoni, G., Bacchi, A., Spasojević, V., Milenković, M., Sladić, D. M., Krstić, N.,& Anđelković, K.. (2014). Synthesis, characterization and antimicrobial activity of Pd(II) and Fe(III) complexes with ethyl (2E)-2-[2-(diphenylphosphino)benzylidene]hydrazinecarboxylate. in Polyhedron, 80(SI), 47-52.
https://doi.org/10.1016/j.poly.2014.01.022

Milenković M, Cantoni G, Bacchi A, Spasojević V, Milenković M, Sladić DM, Krstić N, Anđelković K. Synthesis, characterization and antimicrobial activity of Pd(II) and Fe(III) complexes with ethyl (2E)-2-[2-(diphenylphosphino)benzylidene]hydrazinecarboxylate. in Polyhedron. 2014;80(SI):47-52.
doi:10.1016/j.poly.2014.01.022 .

Milenković, Milica, Cantoni, Giulia, Bacchi, Alessia, Spasojević, Vojislav, Milenković, Marina, Sladić, Dušan M., Krstić, Natalija, Anđelković, Katarina, "Synthesis, characterization and antimicrobial activity of Pd(II) and Fe(III) complexes with ethyl (2E)-2-[2-(diphenylphosphino)benzylidene]hydrazinecarboxylate" in Polyhedron, 80, no. SI (2014):47-52,
https://doi.org/10.1016/j.poly.2014.01.022 . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Ristić, Biljana Z.
AU  - Arsikin, Katarina M.
AU  - Klisic, Djordje G.
AU  - Harhaji-Trajković, Ljubica M.
AU  - Todorović-Marković, Biljana
AU  - Kepić, Dejan P.
AU  - Kravić-Stevović, Tamara K.
AU  - Jovanović, Svetlana P.
AU  - Milenković, Marina
AU  - Milivojević, Dušan
AU  - Bumbaširević, Vladimir Ž.
AU  - Dramićanin, Miroslav
AU  - Trajković, Vladimir S.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5025
AB  - The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470 nm, 1 W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. The cell death induced by photoexcited GQD displayed morphological and/or biochemical characteristics of both apoptosis (phosphatidylserine externalization, caspase activation. DNA fragmentation) and autophagy (formation of autophagic vesicles, LC3-I/LC3-II conversion, degradation of autophagic target p62). Moreover, a genetic inactivation of autophagy-essential LOB protein partly abrogated the photodynamic cytotoxicity of GQD. These data indicate potential usefulness of GQD in photodynamic therapy, but also raise concerns about their possible toxicity. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Biomaterials
T1  - Graphene quantum dots as autophagy-inducing photodynamic agents
VL  - 33
IS  - 29
SP  - 7084
EP  - 7092
DO  - 10.1016/j.biomaterials.2012.06.060
ER  - 

@article{
author = "Marković, Zoran M. and Ristić, Biljana Z. and Arsikin, Katarina M. and Klisic, Djordje G. and Harhaji-Trajković, Ljubica M. and Todorović-Marković, Biljana and Kepić, Dejan P. and Kravić-Stevović, Tamara K. and Jovanović, Svetlana P. and Milenković, Marina and Milivojević, Dušan and Bumbaširević, Vladimir Ž. and Dramićanin, Miroslav and Trajković, Vladimir S.",
year = "2012",
abstract = "The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470 nm, 1 W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. The cell death induced by photoexcited GQD displayed morphological and/or biochemical characteristics of both apoptosis (phosphatidylserine externalization, caspase activation. DNA fragmentation) and autophagy (formation of autophagic vesicles, LC3-I/LC3-II conversion, degradation of autophagic target p62). Moreover, a genetic inactivation of autophagy-essential LOB protein partly abrogated the photodynamic cytotoxicity of GQD. These data indicate potential usefulness of GQD in photodynamic therapy, but also raise concerns about their possible toxicity. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Biomaterials",
title = "Graphene quantum dots as autophagy-inducing photodynamic agents",
volume = "33",
number = "29",
pages = "7084-7092",
doi = "10.1016/j.biomaterials.2012.06.060"
}

Marković, Z. M., Ristić, B. Z., Arsikin, K. M., Klisic, D. G., Harhaji-Trajković, L. M., Todorović-Marković, B., Kepić, D. P., Kravić-Stevović, T. K., Jovanović, S. P., Milenković, M., Milivojević, D., Bumbaširević, V. Ž., Dramićanin, M.,& Trajković, V. S.. (2012). Graphene quantum dots as autophagy-inducing photodynamic agents. in Biomaterials, 33(29), 7084-7092.
https://doi.org/10.1016/j.biomaterials.2012.06.060

Marković ZM, Ristić BZ, Arsikin KM, Klisic DG, Harhaji-Trajković LM, Todorović-Marković B, Kepić DP, Kravić-Stevović TK, Jovanović SP, Milenković M, Milivojević D, Bumbaširević VŽ, Dramićanin M, Trajković VS. Graphene quantum dots as autophagy-inducing photodynamic agents. in Biomaterials. 2012;33(29):7084-7092.
doi:10.1016/j.biomaterials.2012.06.060 .

Marković, Zoran M., Ristić, Biljana Z., Arsikin, Katarina M., Klisic, Djordje G., Harhaji-Trajković, Ljubica M., Todorović-Marković, Biljana, Kepić, Dejan P., Kravić-Stevović, Tamara K., Jovanović, Svetlana P., Milenković, Marina, Milivojević, Dušan, Bumbaširević, Vladimir Ž., Dramićanin, Miroslav, Trajković, Vladimir S., "Graphene quantum dots as autophagy-inducing photodynamic agents" in Biomaterials, 33, no. 29 (2012):7084-7092,
https://doi.org/10.1016/j.biomaterials.2012.06.060 . .