TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Vranješ-Đurić, Sanja
AU  - Mitić, Žarko
AU  - Janković, Drina
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8602
AB  - In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-D,L-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo. The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d(50) = 72 nm are coated with polymers. Radioactive 125-Iodine (I-125), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. I-125-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies. The biodistribution of I-125-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both I-125-HAp/Ch and I-125-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs. (C) 2014 Elsevier B.V. All rights reserved.
T2  - Materials Science and Engineering. C: Materials for Biological Applications
T1  - Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling
VL  - 43
SP  - 439
EP  - 446
DO  - 10.1016/j.msec.2014.07.046
ER  - 

@article{
author = "Ignjatović, Nenad L. and Vranješ-Đurić, Sanja and Mitić, Žarko and Janković, Drina and Uskoković, Dragan",
year = "2014",
abstract = "In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-D,L-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo. The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d(50) = 72 nm are coated with polymers. Radioactive 125-Iodine (I-125), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. I-125-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies. The biodistribution of I-125-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both I-125-HAp/Ch and I-125-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Materials Science and Engineering. C: Materials for Biological Applications",
title = "Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling",
volume = "43",
pages = "439-446",
doi = "10.1016/j.msec.2014.07.046"
}

Ignjatović, N. L., Vranješ-Đurić, S., Mitić, Ž., Janković, D.,& Uskoković, D.. (2014). Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling. in Materials Science and Engineering. C: Materials for Biological Applications, 43, 439-446.
https://doi.org/10.1016/j.msec.2014.07.046

Ignjatović NL, Vranješ-Đurić S, Mitić Ž, Janković D, Uskoković D. Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling. in Materials Science and Engineering. C: Materials for Biological Applications. 2014;43:439-446.
doi:10.1016/j.msec.2014.07.046 .

Ignjatović, Nenad L., Vranješ-Đurić, Sanja, Mitić, Žarko, Janković, Drina, Uskoković, Dragan, "Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling" in Materials Science and Engineering. C: Materials for Biological Applications, 43 (2014):439-446,
https://doi.org/10.1016/j.msec.2014.07.046 . .

TY  - JOUR
AU  - Mitić, Žarko
AU  - Najman, Stevo
AU  - Cakić, Milorad
AU  - Ajduković, Zorica
AU  - Ignjatović, Nenad L.
AU  - Nikolić, Ružica
AU  - Nikolić, Goran
AU  - Stojanović, Sanja
AU  - Vukelić, Marija
AU  - Trajanović, Miroslav
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/554
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7476
AB  - The influence of glucocorticoids on the composition and mineral/organic content of the mandible in tested animals after recovery and healing phase was investigated in this work. The results of FTIR analysis demonstrated that bone tissue composition was changed after glucocorticoid treatment. The increase of calcium, magnesium, phosphorus content and mineral part of bones was statistically significant in recovery phase and in treatment phase that included calcitonin and thymus extract. Some changes also happened in the organic part of the matrix, as indicated by intensity changes for already present IR bands and the appearance of new IR bands in the region 3500–1300 cm−1.
PB  - Elsevier
T2  - Journal of Molecular Structure
T1  - Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period
SP  - 315
EP  - 320
DO  - 10.1016/j.molstruc.2014.06.006
ER  - 

@article{
author = "Mitić, Žarko and Najman, Stevo and Cakić, Milorad and Ajduković, Zorica and Ignjatović, Nenad L. and Nikolić, Ružica and Nikolić, Goran and Stojanović, Sanja and Vukelić, Marija and Trajanović, Miroslav",
year = "2014",
abstract = "The influence of glucocorticoids on the composition and mineral/organic content of the mandible in tested animals after recovery and healing phase was investigated in this work. The results of FTIR analysis demonstrated that bone tissue composition was changed after glucocorticoid treatment. The increase of calcium, magnesium, phosphorus content and mineral part of bones was statistically significant in recovery phase and in treatment phase that included calcitonin and thymus extract. Some changes also happened in the organic part of the matrix, as indicated by intensity changes for already present IR bands and the appearance of new IR bands in the region 3500–1300 cm−1.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure",
title = "Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period",
pages = "315-320",
doi = "10.1016/j.molstruc.2014.06.006"
}

Mitić, Ž., Najman, S., Cakić, M., Ajduković, Z., Ignjatović, N. L., Nikolić, R., Nikolić, G., Stojanović, S., Vukelić, M.,& Trajanović, M.. (2014). Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period. in Journal of Molecular Structure
Elsevier., 315-320.
https://doi.org/10.1016/j.molstruc.2014.06.006

Mitić Ž, Najman S, Cakić M, Ajduković Z, Ignjatović NL, Nikolić R, Nikolić G, Stojanović S, Vukelić M, Trajanović M. Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period. in Journal of Molecular Structure. 2014;:315-320.
doi:10.1016/j.molstruc.2014.06.006 .

Mitić, Žarko, Najman, Stevo, Cakić, Milorad, Ajduković, Zorica, Ignjatović, Nenad L., Nikolić, Ružica, Nikolić, Goran, Stojanović, Sanja, Vukelić, Marija, Trajanović, Miroslav, "Spectroscopic characterization of bone tissue of experimental animals after glucocorticoid treatment and recovery period" in Journal of Molecular Structure (2014):315-320,
https://doi.org/10.1016/j.molstruc.2014.06.006 . .

TY  - JOUR
AU  - Ignjatović, Nenad L.
AU  - Vranješ-Đurić, Sanja
AU  - Mitić, Žarko
AU  - Janković, Drina
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/139
AB  - In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-D,L-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo. The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d(50) = 72 nm are coated with polymers. Radioactive 125-Iodine (I-125), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. I-125-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies. The biodistribution of I-125-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both I-125-HAp/Ch and I-125-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs. (C) 2014 Elsevier B.V. All rights reserved.
T2  - Materials Science and Engineering. C: Materials for Biological Applications
T1  - Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling
VL  - 43
SP  - 439
EP  - 446
DO  - 10.1016/j.msec.2014.07.046
ER  - 

@article{
author = "Ignjatović, Nenad L. and Vranješ-Đurić, Sanja and Mitić, Žarko and Janković, Drina and Uskoković, Dragan",
year = "2014",
abstract = "In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite (HAp) and hydroxyapatite coated with chitosan (HAp/Ch) and the chitosan-poly-D,L-lactide-co-glycolide polymer blend (HAp/Ch-PLGA) as an organ-targeting system. We have examined and defined the final destination, as well as the dynamics and the pathways of the synthesized particles following intravenous administration in vivo. The XRD, ZP, FT-IR and SEM analyses have confirmed that the hydroxyapatite nanoparticles with d(50) = 72 nm are coated with polymers. Radioactive 125-Iodine (I-125), a low energy gamma emitter, was used to develop a novel in situ method for the radiolabeling of particles and investigation of their biodistribution. I-125-labeled particles exhibited high stability in saline and serum over the second day, which justified their use in the following in vivo studies. The biodistribution of I-125-labeled particles after intravenous injection in rats differed significantly: HAp particles mostly targeted the liver, HAp/Ch the spleen and the liver, while HAp/Ch-PLGA targeted the lungs. Twenty-four hours post injection, HAp particles were excreted completely, while both I-125-HAp/Ch and I-125-HAp/Ch-PLGA were retained in the body for a prolonged period of time with more than 20% of radioactivity still found in different organs. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Materials Science and Engineering. C: Materials for Biological Applications",
title = "Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling",
volume = "43",
pages = "439-446",
doi = "10.1016/j.msec.2014.07.046"
}

Ignjatović, N. L., Vranješ-Đurić, S., Mitić, Ž., Janković, D.,& Uskoković, D.. (2014). Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling. in Materials Science and Engineering. C: Materials for Biological Applications, 43, 439-446.
https://doi.org/10.1016/j.msec.2014.07.046

Ignjatović NL, Vranješ-Đurić S, Mitić Ž, Janković D, Uskoković D. Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling. in Materials Science and Engineering. C: Materials for Biological Applications. 2014;43:439-446.
doi:10.1016/j.msec.2014.07.046 .

Ignjatović, Nenad L., Vranješ-Đurić, Sanja, Mitić, Žarko, Janković, Drina, Uskoković, Dragan, "Investigating an organ-targeting platform based on hydroxyapatite nanoparticles using a novel in situ method of radioactive (125)Iodine labeling" in Materials Science and Engineering. C: Materials for Biological Applications, 43 (2014):439-446,
https://doi.org/10.1016/j.msec.2014.07.046 . .

TY  - CONF
AU  - Ignjatović, Nenad L.
AU  - Vranješ-Đurić, Sanja
AU  - Mitić, Žarko
AU  - Janković, Drina
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://dais.sanu.ac.rs/123456789/393
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7415
AB  - Nanoparticles based on hydroxyapatite (NPs) have many useful physicochemical and biologicalproperties, such as easy preparation and modification, as well as biocompatibility, which make them suitable for transport and unloading of various pharmaceuticals. Hydroxyapatitenanoparticles coated with bioresorbable polymers have been successfully used as carriers ofantibiotics and vitamins in bone tissue engineering. In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite and hydroxyapatite coated with chitosan and the chitosan-poly-D,L-lactide-co-glycolide polymer blend. The influence of the processing technique on the structure and characteristics of the obtained particles was studied by X-ray diffraction (XRD), particle size distribution analysis (PSD), Fourier transform infrared spectroscopy (FTIR), zeta potential analysis (ZP) and scanning electronic microscopy (SEM). In applied research, an appropriate radioisotope (Iodine-125) was selected and it was used to label particles. The in vivo biodistribution of 125I-labeled particles were studied in healthy Wistar rats following intravenous administration. The XRD, FT-IR and ZP analyses have confirmed that the hydroxyapatite particles with d50=72 nm are coated with chitosan and the chitosan-poly-D,L-lactide-co-glycolide polymer blend. 125I-labeled particles showed completely different behaviour in vivo: hydroxyapatite particles have the highest liver accumulation 10 min after injection but rapid excretion from the body without residual radioactivity 24 hours after injection; chitozan coated hydroxyapatite particles have the highest accumulation in the liver 10 min after injection with considerable amount (almost 50 %) retained 24 hours later; hydroxyapatite particles coated with the chitosanpoly-D,L-lactide-co-glycolide polymer blend have the highest uptake in the lungs 10 minutes after injection and moderate retention in the same organ 24 hours later.
PB  - Belgrade : Materials Research Society of Serbia
C3  - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
T1  - Routes and pathways to small particles based on hydroxyapatite
SP  - 7
EP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7415
ER  - 

@conference{
author = "Ignjatović, Nenad L. and Vranješ-Đurić, Sanja and Mitić, Žarko and Janković, Drina and Uskoković, Dragan",
year = "2013",
abstract = "Nanoparticles based on hydroxyapatite (NPs) have many useful physicochemical and biologicalproperties, such as easy preparation and modification, as well as biocompatibility, which make them suitable for transport and unloading of various pharmaceuticals. Hydroxyapatitenanoparticles coated with bioresorbable polymers have been successfully used as carriers ofantibiotics and vitamins in bone tissue engineering. In this study, we have investigated the synthesis of nanoparticles of hydroxyapatite and hydroxyapatite coated with chitosan and the chitosan-poly-D,L-lactide-co-glycolide polymer blend. The influence of the processing technique on the structure and characteristics of the obtained particles was studied by X-ray diffraction (XRD), particle size distribution analysis (PSD), Fourier transform infrared spectroscopy (FTIR), zeta potential analysis (ZP) and scanning electronic microscopy (SEM). In applied research, an appropriate radioisotope (Iodine-125) was selected and it was used to label particles. The in vivo biodistribution of 125I-labeled particles were studied in healthy Wistar rats following intravenous administration. The XRD, FT-IR and ZP analyses have confirmed that the hydroxyapatite particles with d50=72 nm are coated with chitosan and the chitosan-poly-D,L-lactide-co-glycolide polymer blend. 125I-labeled particles showed completely different behaviour in vivo: hydroxyapatite particles have the highest liver accumulation 10 min after injection but rapid excretion from the body without residual radioactivity 24 hours after injection; chitozan coated hydroxyapatite particles have the highest accumulation in the liver 10 min after injection with considerable amount (almost 50 %) retained 24 hours later; hydroxyapatite particles coated with the chitosanpoly-D,L-lactide-co-glycolide polymer blend have the highest uptake in the lungs 10 minutes after injection and moderate retention in the same organ 24 hours later.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts",
title = "Routes and pathways to small particles based on hydroxyapatite",
pages = "7-7",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7415"
}

Ignjatović, N. L., Vranješ-Đurić, S., Mitić, Ž., Janković, D.,& Uskoković, D.. (2013). Routes and pathways to small particles based on hydroxyapatite. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 7-7.
https://hdl.handle.net/21.15107/rcub_vinar_7415

Ignjatović NL, Vranješ-Đurić S, Mitić Ž, Janković D, Uskoković D. Routes and pathways to small particles based on hydroxyapatite. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:7-7.
https://hdl.handle.net/21.15107/rcub_vinar_7415 .

Ignjatović, Nenad L., Vranješ-Đurić, Sanja, Mitić, Žarko, Janković, Drina, Uskoković, Dragan, "Routes and pathways to small particles based on hydroxyapatite" in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):7-7,
https://hdl.handle.net/21.15107/rcub_vinar_7415 .

TY  - JOUR
AU  - Vukelić, Marija
AU  - Mitić, Žarko
AU  - Miljković, Miroslav
AU  - Živković, Jelena
AU  - Ignjatović, Nenad L.
AU  - Uskoković, Dragan
AU  - Živanov Čurlis, Jelena
AU  - Vasiljević, Perica
AU  - Najman, Stevo
PY  - 2012
UR  - http://dais.sanu.ac.rs/123456789/489
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7451
AB  - Simulated body fluid (SBF) is an artificial fluid which has ionic composition and ionic concentration similar to human blood plasma. Purpose: This paper compares the interaction between the nanomaterial containing calcium phosphate/poly-dl-lactide-co-glycolide (N-CP/PLGA) and SBF, in order to investigate whether and to what extent inorganic ionic composition of human blood plasma leads to the aforementioned changes in the material. Methods: N-CP/PLGA was incubated for 1, 2, 3, and 5 weeks in SBF. The surface of the material was analyzed on SEM-EDS and FTIR spectrometer, while SBF was subjected to pH and electrical conductivity measurement. Results: Our results indicate that dissolution of the polymer component of the material N-CP/PLGA and precipitation of the material similar to hydroxyapatite on its surface are based on the morphologic changes seen in this material. Conclusions: The mechanism of the apatite formation on the bioceramic surface was intensively studied and was considered crucial in designing the new biomaterials. The results obtained in this work indicate that N-CP/PLGA may be a good candidate for application to bone regeneration.
PB  - Milano : Wichtig Publishing
T2  - Journal of Applied Biomaterials and Functional Materials
T1  - Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid
SP  - 43
EP  - 48
DO  - 10.5301/JABFM.2012.9274
ER  - 

@article{
author = "Vukelić, Marija and Mitić, Žarko and Miljković, Miroslav and Živković, Jelena and Ignjatović, Nenad L. and Uskoković, Dragan and Živanov Čurlis, Jelena and Vasiljević, Perica and Najman, Stevo",
year = "2012",
abstract = "Simulated body fluid (SBF) is an artificial fluid which has ionic composition and ionic concentration similar to human blood plasma. Purpose: This paper compares the interaction between the nanomaterial containing calcium phosphate/poly-dl-lactide-co-glycolide (N-CP/PLGA) and SBF, in order to investigate whether and to what extent inorganic ionic composition of human blood plasma leads to the aforementioned changes in the material. Methods: N-CP/PLGA was incubated for 1, 2, 3, and 5 weeks in SBF. The surface of the material was analyzed on SEM-EDS and FTIR spectrometer, while SBF was subjected to pH and electrical conductivity measurement. Results: Our results indicate that dissolution of the polymer component of the material N-CP/PLGA and precipitation of the material similar to hydroxyapatite on its surface are based on the morphologic changes seen in this material. Conclusions: The mechanism of the apatite formation on the bioceramic surface was intensively studied and was considered crucial in designing the new biomaterials. The results obtained in this work indicate that N-CP/PLGA may be a good candidate for application to bone regeneration.",
publisher = "Milano : Wichtig Publishing",
journal = "Journal of Applied Biomaterials and Functional Materials",
title = "Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid",
pages = "43-48",
doi = "10.5301/JABFM.2012.9274"
}

Vukelić, M., Mitić, Ž., Miljković, M., Živković, J., Ignjatović, N. L., Uskoković, D., Živanov Čurlis, J., Vasiljević, P.,& Najman, S.. (2012). Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid. in Journal of Applied Biomaterials and Functional Materials
Milano : Wichtig Publishing., 43-48.
https://doi.org/10.5301/JABFM.2012.9274

Vukelić M, Mitić Ž, Miljković M, Živković J, Ignjatović NL, Uskoković D, Živanov Čurlis J, Vasiljević P, Najman S. Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid. in Journal of Applied Biomaterials and Functional Materials. 2012;:43-48.
doi:10.5301/JABFM.2012.9274 .

Vukelić, Marija, Mitić, Žarko, Miljković, Miroslav, Živković, Jelena, Ignjatović, Nenad L., Uskoković, Dragan, Živanov Čurlis, Jelena, Vasiljević, Perica, Najman, Stevo, "Apatite formation on nanomaterial calcium phosphate/poly-DL-lactide-co-glycolide in simulated body fluid" in Journal of Applied Biomaterials and Functional Materials (2012):43-48,
https://doi.org/10.5301/JABFM.2012.9274 . .

TY  - JOUR
AU  - Vukelić, Marija
AU  - Mitić, Žarko
AU  - Miljković, Miroslav
AU  - Živković, Jelena
AU  - Ignjatović, Nenad L.
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/701
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7521
AB  - The purpose of biomaterials is to replace a part or a function of the body in a safe, physiologically and economically acceptable way. The process of the reconstruction of bone defects has always been a big problem in orthopedics and maxillofacial surgery. Since hydroxyapatite (HAp) was detected as a component, the predominant constituent and the integral element of Mammalian bones, the development of the phospate ceramics as potential materials for implantation was enabled. This study investigated whether and in which way biomaterial calcium hydroxyapatite/poly-L-lactide (HAp/PLLA) interacts with the ionic composition of the human plasma. The simulated body fluid (SBF) is an artificial fluid that has the ionic composition and ionic concentration similar to the human blood plasma. HAp/PLLA was incubated for 1, 2, 3 and 5 weeks in SBF. The surfaces of both treated and untreated materials were analyzed on a scanning electron microscopy (SEM), and were also exposed to the energy dispersive X-ray spectroscopy (EDS), while SBF was submitted to the measuring of pH and electrical conductivity. However, our results indicate that the degradational changes of the material HAp/PLLA in SBF start from the surface of the treated material and that observed changes are the consequence of dissolution of its polymer component and the precipitation of the material similar to hydroxyapatite on its surface. This material shows good characteristics that place it among good candidates for the application in orthopedics and maxillofacial surgery.
PB  - Niš : University of Niš, Faculty of Medicine; Department of  the Serbian Medical Society Niš
T2  - Acta Medica Medianae
T1  - Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid
SP  - 35
EP  - 39
DO  - 10.5633/amm.2011.0406
ER  - 

@article{
author = "Vukelić, Marija and Mitić, Žarko and Miljković, Miroslav and Živković, Jelena and Ignjatović, Nenad L. and Uskoković, Dragan",
year = "2011",
abstract = "The purpose of biomaterials is to replace a part or a function of the body in a safe, physiologically and economically acceptable way. The process of the reconstruction of bone defects has always been a big problem in orthopedics and maxillofacial surgery. Since hydroxyapatite (HAp) was detected as a component, the predominant constituent and the integral element of Mammalian bones, the development of the phospate ceramics as potential materials for implantation was enabled. This study investigated whether and in which way biomaterial calcium hydroxyapatite/poly-L-lactide (HAp/PLLA) interacts with the ionic composition of the human plasma. The simulated body fluid (SBF) is an artificial fluid that has the ionic composition and ionic concentration similar to the human blood plasma. HAp/PLLA was incubated for 1, 2, 3 and 5 weeks in SBF. The surfaces of both treated and untreated materials were analyzed on a scanning electron microscopy (SEM), and were also exposed to the energy dispersive X-ray spectroscopy (EDS), while SBF was submitted to the measuring of pH and electrical conductivity. However, our results indicate that the degradational changes of the material HAp/PLLA in SBF start from the surface of the treated material and that observed changes are the consequence of dissolution of its polymer component and the precipitation of the material similar to hydroxyapatite on its surface. This material shows good characteristics that place it among good candidates for the application in orthopedics and maxillofacial surgery.",
publisher = "Niš : University of Niš, Faculty of Medicine; Department of  the Serbian Medical Society Niš",
journal = "Acta Medica Medianae",
title = "Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid",
pages = "35-39",
doi = "10.5633/amm.2011.0406"
}

Vukelić, M., Mitić, Ž., Miljković, M., Živković, J., Ignjatović, N. L.,& Uskoković, D.. (2011). Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid. in Acta Medica Medianae
Niš : University of Niš, Faculty of Medicine; Department of  the Serbian Medical Society Niš., 35-39.
https://doi.org/10.5633/amm.2011.0406

Vukelić M, Mitić Ž, Miljković M, Živković J, Ignjatović NL, Uskoković D. Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid. in Acta Medica Medianae. 2011;:35-39.
doi:10.5633/amm.2011.0406 .

Vukelić, Marija, Mitić, Žarko, Miljković, Miroslav, Živković, Jelena, Ignjatović, Nenad L., Uskoković, Dragan, "Interaction of biomaterial containing calcium hydroxyapatite/poly-l-lactide with simulated body fluid" in Acta Medica Medianae (2011):35-39,
https://doi.org/10.5633/amm.2011.0406 . .