TY  - CONF
AU  - Čolović, Mirjana B.
AU  - Ma, Tian
AU  - Ma, Xiang
AU  - Isaković, Anđelka
AU  - Misirlić-Denčić, Sonja
AU  - Kortz, Urlich
AU  - Krstić, Danijela
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13018
AB  - Polyoxopalladates (POPs) are discrete, anionic palladium(II)- oxo nanoclusters combining properties of polyoxometalates and palladium(II), and thus are highly promising for the development of novel antitumor metallodrugs. The aim of this study was to investigate in vitro the influence of three POP salts with approved anti-neuroblastoma action, Na8[Pd13As8O34(OH)6]·42H2O (Pd13), Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3]·2NaOAc·32H2O (SrPd12), and Na6[Pd13O8(PhAsO3)8]·23H2O (Pd13L), on E-NTPDase activity using rat synaptic plasma membranes (SPMs) isolated from Wistar brain as a model system. Dose-dependent inhibition of ENTPDases was obtained within concentration range 2 × 10-6 - 1 × 10-3 mol/L for all investigated POPs. Inhibition parameters, IC50 value and Hill's coefficient, nH, were determined by sigmoidal fitting the experimental results. The calculated IC50 values were (1.08 ± 0.25) × 10-4 , (1.19 ± 0.13) × 10-4 , and (2.06 ± 0.88) × 10-4 mol/L for Pd13, SrPd12, and Pd13L, respectively, indicating their similar inhibitory strengths. The nH values were determined to be < 1, indicating negatively cooperative binding for all POPs studied. The observed inhibitory effect of these anti-neuroblastoma POPs on ENTPDase activity suggest that the inhibition of E-NTPDases, the enzymes representing the major part of purinergic signaling, could be considered as a putative mechanism of antitumor action and a new strategy in the development of novel antitumor therapeutics.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts
T1  - Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases
SP  - 77
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13018
ER  - 

@conference{
author = "Čolović, Mirjana B. and Ma, Tian and Ma, Xiang and Isaković, Anđelka and Misirlić-Denčić, Sonja and Kortz, Urlich and Krstić, Danijela",
year = "2021",
abstract = "Polyoxopalladates (POPs) are discrete, anionic palladium(II)- oxo nanoclusters combining properties of polyoxometalates and palladium(II), and thus are highly promising for the development of novel antitumor metallodrugs. The aim of this study was to investigate in vitro the influence of three POP salts with approved anti-neuroblastoma action, Na8[Pd13As8O34(OH)6]·42H2O (Pd13), Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3]·2NaOAc·32H2O (SrPd12), and Na6[Pd13O8(PhAsO3)8]·23H2O (Pd13L), on E-NTPDase activity using rat synaptic plasma membranes (SPMs) isolated from Wistar brain as a model system. Dose-dependent inhibition of ENTPDases was obtained within concentration range 2 × 10-6 - 1 × 10-3 mol/L for all investigated POPs. Inhibition parameters, IC50 value and Hill's coefficient, nH, were determined by sigmoidal fitting the experimental results. The calculated IC50 values were (1.08 ± 0.25) × 10-4 , (1.19 ± 0.13) × 10-4 , and (2.06 ± 0.88) × 10-4 mol/L for Pd13, SrPd12, and Pd13L, respectively, indicating their similar inhibitory strengths. The nH values were determined to be < 1, indicating negatively cooperative binding for all POPs studied. The observed inhibitory effect of these anti-neuroblastoma POPs on ENTPDase activity suggest that the inhibition of E-NTPDases, the enzymes representing the major part of purinergic signaling, could be considered as a putative mechanism of antitumor action and a new strategy in the development of novel antitumor therapeutics.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts",
title = "Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases",
pages = "77-77",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13018"
}

Čolović, M. B., Ma, T., Ma, X., Isaković, A., Misirlić-Denčić, S., Kortz, U.,& Krstić, D.. (2021). Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts
Belgrade : Society of Physical Chemists of Serbia., 77-77.
https://hdl.handle.net/21.15107/rcub_vinar_13018

Čolović MB, Ma T, Ma X, Isaković A, Misirlić-Denčić S, Kortz U, Krstić D. Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts. 2021;:77-77.
https://hdl.handle.net/21.15107/rcub_vinar_13018 .

Čolović, Mirjana B., Ma, Tian, Ma, Xiang, Isaković, Anđelka, Misirlić-Denčić, Sonja, Kortz, Urlich, Krstić, Danijela, "Influence of polyoxopalladates(II) on ecto-nucleoside triphosphate diphosphohydrolases" in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts (2021):77-77,
https://hdl.handle.net/21.15107/rcub_vinar_13018 .

TY  - CONF
AU  - Čolović, Mirjana B.
AU  - Ma, Tian
AU  - Ma, Xiang
AU  - Isaković, Anđelka
AU  - Misirlić-Denčić, Sonja
AU  - Kortz, Urlich
AU  - Krstić, Danijela
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13019
AB  - Polyoxopalladates(II) (POPs) are the largest subset of polyoxo-noble-metalates (PONMs), representing a class of discrete, anionic noble metal-oxo nanoclusters. In this study, the in vitro effects of two isostructural, fully inorganic POP salts containing tetravalent metal ions (SnIV and PbIV) incorporated inside the cubic Pd12-oxo host-shell, Na12[SnIVO8Pd12(PO4)8]·43H2O (SnPd12) and Na12[PbIVO8Pd12(PO4)8]·38H2O (PbPd12), which were found to exhibit considerable antileukemic effects, on E-NTPDase activity were investigated using rat synaptic plasma membranes (SPMs) as a model system. Concentration-dependent inhibition of E-NTPDases was observed within the concentration range 5 × 10-6 - 2 × 10-4 mol/L for both POPs. Inhibition parameters, half-maximum inhibitory concentrations (IC50 values) and Hill's coefficients, nH, were determined by sigmoidal fitting the experimental results and Hill's analysis. The calculated IC50 values were (6.59 ± 1.09) × 10-5 and (9.88 ± 3.83) × 10-5 mol/L for SnPd12 and PbPd12, respectively. The calculated nH values were < 1, indicating negatively cooperative enzyme-inhibitor binding for both POPs. Accordingly, the confirmed antileukemic activities of SnPd12 and PbPd12 could be associated with the observed inhibition of E-NTPDases as a potential target of the antileukemic action of these promising drug candidates.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts
T1  - Inhibition of ecto-nucleoside triphosphate diphosphohydrolases by polyoxopalladates with promising antileukemic properties
SP  - 78
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13019
ER  - 

@conference{
author = "Čolović, Mirjana B. and Ma, Tian and Ma, Xiang and Isaković, Anđelka and Misirlić-Denčić, Sonja and Kortz, Urlich and Krstić, Danijela",
year = "2021",
abstract = "Polyoxopalladates(II) (POPs) are the largest subset of polyoxo-noble-metalates (PONMs), representing a class of discrete, anionic noble metal-oxo nanoclusters. In this study, the in vitro effects of two isostructural, fully inorganic POP salts containing tetravalent metal ions (SnIV and PbIV) incorporated inside the cubic Pd12-oxo host-shell, Na12[SnIVO8Pd12(PO4)8]·43H2O (SnPd12) and Na12[PbIVO8Pd12(PO4)8]·38H2O (PbPd12), which were found to exhibit considerable antileukemic effects, on E-NTPDase activity were investigated using rat synaptic plasma membranes (SPMs) as a model system. Concentration-dependent inhibition of E-NTPDases was observed within the concentration range 5 × 10-6 - 2 × 10-4 mol/L for both POPs. Inhibition parameters, half-maximum inhibitory concentrations (IC50 values) and Hill's coefficients, nH, were determined by sigmoidal fitting the experimental results and Hill's analysis. The calculated IC50 values were (6.59 ± 1.09) × 10-5 and (9.88 ± 3.83) × 10-5 mol/L for SnPd12 and PbPd12, respectively. The calculated nH values were < 1, indicating negatively cooperative enzyme-inhibitor binding for both POPs. Accordingly, the confirmed antileukemic activities of SnPd12 and PbPd12 could be associated with the observed inhibition of E-NTPDases as a potential target of the antileukemic action of these promising drug candidates.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts",
title = "Inhibition of ecto-nucleoside triphosphate diphosphohydrolases by polyoxopalladates with promising antileukemic properties",
pages = "78-78",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13019"
}

Čolović, M. B., Ma, T., Ma, X., Isaković, A., Misirlić-Denčić, S., Kortz, U.,& Krstić, D.. (2021). Inhibition of ecto-nucleoside triphosphate diphosphohydrolases by polyoxopalladates with promising antileukemic properties. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts
Belgrade : Society of Physical Chemists of Serbia., 78-78.
https://hdl.handle.net/21.15107/rcub_vinar_13019

Čolović MB, Ma T, Ma X, Isaković A, Misirlić-Denčić S, Kortz U, Krstić D. Inhibition of ecto-nucleoside triphosphate diphosphohydrolases by polyoxopalladates with promising antileukemic properties. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts. 2021;:78-78.
https://hdl.handle.net/21.15107/rcub_vinar_13019 .

Čolović, Mirjana B., Ma, Tian, Ma, Xiang, Isaković, Anđelka, Misirlić-Denčić, Sonja, Kortz, Urlich, Krstić, Danijela, "Inhibition of ecto-nucleoside triphosphate diphosphohydrolases by polyoxopalladates with promising antileukemic properties" in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry : Book of abstracts (2021):78-78,
https://hdl.handle.net/21.15107/rcub_vinar_13019 .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Gajski, Goran
AU  - Ma, Tian
AU  - Isaković, Anđelka
AU  - Misirlić-Denčić, Sonja
AU  - Kortz, Urlich
AU  - Krstić, Danijela
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12932
AB  - Polyoxometalates (POMs) are discrete, negatively charged metal-oxo clusters of early transition metal ions in high oxidation states. Their biological significance has greatly increased in recent years because of their approved anticancer, antibiotic, and antidiabetic properties. However, toxicity studies have reported adverse effects after in vivo POM studies, which limits their potential application in biomedicine. The aim of this study is to evaluate the in vitro cyto- and genotoxic properties of two polyoxopalladates(II) containing tetravalent metal ion guests, SnPd12 and PbPd12, that were found to possess potent antitumor activities against the human acute promyelocytic cell line HL-60. For this purpose, blood samples obtained from a healthy female donor were treated with three different concentrations (12.5, 25, and 50 µmol/L) of the tested POPs, and incubated at 37 o C for 4 and 24 h, respectively. Cytotoxicity studies were performed on isolated human peripheral blood lymphocytes which were stained with acridine orange and ethidium bromide, and then viewed under a fluorescence microscope. The genotoxicity was tested in whole blood by alkaline comet assay (microgel electrophoresis). The percentage of tail DNA was used to determine the level of DNA damage. The obtained cytotoxicity results indicated that neither SnPd12 nor PbPd12 induced statistically significant alterations of cell viability related to the control, at all of the investigated concentrations. Moreover, the results of the comet assay showed that none of the tested POPs resulted in a statistically significant relative increase of tail DNA. Accordingly, both SnPd12 and PbPd12 could be considered as safe promising antileukaemic drugs from a cyto/genotoxicity point of view.
PB  - Zagreb, Croatia : Institute for Medical Research and Occupational Health
C3  - Archives of Industrial Hygiene and Toxicology
T1  - Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs
VL  - 72
IS  - Suppl. 1
SP  - 66
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12932
ER  - 

@conference{
author = "Čolović, Mirjana and Gajski, Goran and Ma, Tian and Isaković, Anđelka and Misirlić-Denčić, Sonja and Kortz, Urlich and Krstić, Danijela",
year = "2021",
abstract = "Polyoxometalates (POMs) are discrete, negatively charged metal-oxo clusters of early transition metal ions in high oxidation states. Their biological significance has greatly increased in recent years because of their approved anticancer, antibiotic, and antidiabetic properties. However, toxicity studies have reported adverse effects after in vivo POM studies, which limits their potential application in biomedicine. The aim of this study is to evaluate the in vitro cyto- and genotoxic properties of two polyoxopalladates(II) containing tetravalent metal ion guests, SnPd12 and PbPd12, that were found to possess potent antitumor activities against the human acute promyelocytic cell line HL-60. For this purpose, blood samples obtained from a healthy female donor were treated with three different concentrations (12.5, 25, and 50 µmol/L) of the tested POPs, and incubated at 37 o C for 4 and 24 h, respectively. Cytotoxicity studies were performed on isolated human peripheral blood lymphocytes which were stained with acridine orange and ethidium bromide, and then viewed under a fluorescence microscope. The genotoxicity was tested in whole blood by alkaline comet assay (microgel electrophoresis). The percentage of tail DNA was used to determine the level of DNA damage. The obtained cytotoxicity results indicated that neither SnPd12 nor PbPd12 induced statistically significant alterations of cell viability related to the control, at all of the investigated concentrations. Moreover, the results of the comet assay showed that none of the tested POPs resulted in a statistically significant relative increase of tail DNA. Accordingly, both SnPd12 and PbPd12 could be considered as safe promising antileukaemic drugs from a cyto/genotoxicity point of view.",
publisher = "Zagreb, Croatia : Institute for Medical Research and Occupational Health",
journal = "Archives of Industrial Hygiene and Toxicology",
title = "Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs",
volume = "72",
number = "Suppl. 1",
pages = "66-66",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12932"
}

Čolović, M., Gajski, G., Ma, T., Isaković, A., Misirlić-Denčić, S., Kortz, U.,& Krstić, D.. (2021). Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs. in Archives of Industrial Hygiene and Toxicology
Zagreb, Croatia : Institute for Medical Research and Occupational Health., 72(Suppl. 1), 66-66.
https://hdl.handle.net/21.15107/rcub_vinar_12932

Čolović M, Gajski G, Ma T, Isaković A, Misirlić-Denčić S, Kortz U, Krstić D. Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs. in Archives of Industrial Hygiene and Toxicology. 2021;72(Suppl. 1):66-66.
https://hdl.handle.net/21.15107/rcub_vinar_12932 .

Čolović, Mirjana, Gajski, Goran, Ma, Tian, Isaković, Anđelka, Misirlić-Denčić, Sonja, Kortz, Urlich, Krstić, Danijela, "Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs" in Archives of Industrial Hygiene and Toxicology, 72, no. Suppl. 1 (2021):66-66,
https://hdl.handle.net/21.15107/rcub_vinar_12932 .

TY  - CONF
AU  - Čolović, Mirjana
AU  - Gajski, Goran
AU  - Ma, Tian
AU  - Isaković, Anđelka
AU  - Misirlić-Denčić, Sonja
AU  - Kortz, Urlich
AU  - Krstić, Danijela
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12963
AB  - Polyoxopalladates (POPs) are a subclass of polyoxometalates (POMs) comprising discrete, anionic palladium(II)-oxo complexes. Although numerous studies have been conducted on the biological activity of POMs, their toxicity (e.g. nonselectivity) is frequently a limitation for real-world biomedical applications. Thus, the aim of this study was to evaluate the in vitro safety of the three POPs Pd13 As8 , SrPd12 As6 , and Pd13 (PhAs)8 , which exhibited strong antitumour activity against human neuroblastoma cell line SH-SY5Y, by performing a cyto/genotoxicity study on human healthy blood. Blood samples obtained from a healthy female donor were treated with three different concentrations (12.5, 25, and 50 µmol/L) of the POPs, and incubated at 37 oC for 4 and 24 h. A cytotoxicity (cell viability) assay was performed on isolated human peripheral blood lymphocytes stained with acridine orange and ethidium bromide. A genotoxicity test was carried out on whole blood by alkaline comet assay (microgel electrophoresis), and the percentage of tail DNA was used to assess the level of DNA damage. Pd13 As8 did not affect neither cell viability nor DNA damage, related to the control, at either of the investigated concentrations (after both 4 and 24 h). On the contrary, higher concentrations (25 and 50 µmol/L) of both SrPd12 As6 and Pd13 (PhAs)8 induced a statistically significant decrease in cell viability after 24 h (up to 42 %), and a relative increase of tail DNA (up to 3×) was observed at 50 µmol/L, after 24 h. Therefore, Pd13 As8 could be regarded as non-toxic to human healthy cells, whereas SrPd12 As6 and Pd13 (PhAs)8 require additional toxicity analysis.
PB  - Zagreb, Croatia : Institute for Medical Research and Occupational Health
C3  - Archives of Industrial Hygiene and Toxicology
T1  - Polyoxopalladates as potential antitumor drugs: in vitro toxicity assessment
VL  - 72
IS  - Suppl. 1
SP  - 65
EP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12963
ER  - 

@conference{
author = "Čolović, Mirjana and Gajski, Goran and Ma, Tian and Isaković, Anđelka and Misirlić-Denčić, Sonja and Kortz, Urlich and Krstić, Danijela",
year = "2021",
abstract = "Polyoxopalladates (POPs) are a subclass of polyoxometalates (POMs) comprising discrete, anionic palladium(II)-oxo complexes. Although numerous studies have been conducted on the biological activity of POMs, their toxicity (e.g. nonselectivity) is frequently a limitation for real-world biomedical applications. Thus, the aim of this study was to evaluate the in vitro safety of the three POPs Pd13 As8 , SrPd12 As6 , and Pd13 (PhAs)8 , which exhibited strong antitumour activity against human neuroblastoma cell line SH-SY5Y, by performing a cyto/genotoxicity study on human healthy blood. Blood samples obtained from a healthy female donor were treated with three different concentrations (12.5, 25, and 50 µmol/L) of the POPs, and incubated at 37 oC for 4 and 24 h. A cytotoxicity (cell viability) assay was performed on isolated human peripheral blood lymphocytes stained with acridine orange and ethidium bromide. A genotoxicity test was carried out on whole blood by alkaline comet assay (microgel electrophoresis), and the percentage of tail DNA was used to assess the level of DNA damage. Pd13 As8 did not affect neither cell viability nor DNA damage, related to the control, at either of the investigated concentrations (after both 4 and 24 h). On the contrary, higher concentrations (25 and 50 µmol/L) of both SrPd12 As6 and Pd13 (PhAs)8 induced a statistically significant decrease in cell viability after 24 h (up to 42 %), and a relative increase of tail DNA (up to 3×) was observed at 50 µmol/L, after 24 h. Therefore, Pd13 As8 could be regarded as non-toxic to human healthy cells, whereas SrPd12 As6 and Pd13 (PhAs)8 require additional toxicity analysis.",
publisher = "Zagreb, Croatia : Institute for Medical Research and Occupational Health",
journal = "Archives of Industrial Hygiene and Toxicology",
title = "Polyoxopalladates as potential antitumor drugs: in vitro toxicity assessment",
volume = "72",
number = "Suppl. 1",
pages = "65-65",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12963"
}

Čolović, M., Gajski, G., Ma, T., Isaković, A., Misirlić-Denčić, S., Kortz, U.,& Krstić, D.. (2021). Polyoxopalladates as potential antitumor drugs: in vitro toxicity assessment. in Archives of Industrial Hygiene and Toxicology
Zagreb, Croatia : Institute for Medical Research and Occupational Health., 72(Suppl. 1), 65-65.
https://hdl.handle.net/21.15107/rcub_vinar_12963

Čolović M, Gajski G, Ma T, Isaković A, Misirlić-Denčić S, Kortz U, Krstić D. Polyoxopalladates as potential antitumor drugs: in vitro toxicity assessment. in Archives of Industrial Hygiene and Toxicology. 2021;72(Suppl. 1):65-65.
https://hdl.handle.net/21.15107/rcub_vinar_12963 .

Čolović, Mirjana, Gajski, Goran, Ma, Tian, Isaković, Anđelka, Misirlić-Denčić, Sonja, Kortz, Urlich, Krstić, Danijela, "Polyoxopalladates as potential antitumor drugs: in vitro toxicity assessment" in Archives of Industrial Hygiene and Toxicology, 72, no. Suppl. 1 (2021):65-65,
https://hdl.handle.net/21.15107/rcub_vinar_12963 .

TY  - CONF
AU  - Isaković, Anđelka
AU  - Jeremić, Marija
AU  - Krstić, Danijela
AU  - Čolović, Mirjana
AU  - Kortz, Ulrich
AU  - Misirlić-Denčić, Sonja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12964
AB  - Background: Polyoxometalates are a class of anionic, polynuclear metal-oxo clusters reported as promising in vitro and in vivo antitumor agents for several decades. The aim of this study was to investigate the antineuroblastoma potential of the polyoxopalladate(II) nanocube Na8[Pd13As8O34(OH)6]・42H2O (Pd13). Material and methods: All experiments were performed on human neuroblastoma cell line, SH-SY5Y. The number of viable cells after the treatment with Pd13 was assessed using an acid phosphatase viability assay. The level of superoxide ion, mitochondrial membrane potential, pan-caspase activity, acidic intracellular vesicles content, and the cell cycle was determined by flow cytometry. Results: The obtained results suggest that Pd13 caused a significant decrease in cell viability with IC50 values of 7.7 µM (24 h) and 4.4 µM (48 h). Pd13 induced depolarization of mitochondrial membrane (2 h), followed by ~ 30% increase in the production of the superoxide ion (O2-) 4 h after treatment. An increase (~ 30%) in pancaspase activation and disturbance of neuroblastma cell cycle were observed after 24 h treatment. Namely, Pd13 caused an increase (14.4%) in the number of cells with fragmented nuclear DNA (SubG0), a decrease (%) of cells in the G1 phase, and an increase (%) in the S phase, all suggestive of cell cycle arrest. Finally, Pd13 increased the orange to green fluorescence ratio for ~ 45% 24 h after treatment, supporting intracellular acidification. Conclusion: The polyoxopalladate, Pd13 can be regarded as a promising antineuroblastoma agent which induces oxidative stress, and causes pan-caspase activation, DNA fragmentation and cell cycle arrest, which are all hallmarks of apoptotic neuroblastoma cell death.
PB  - Belgrade : Serbian Association for Cancer Research (SDIR)
C3  - SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
T1  - Antineuroblastoma potential of polyoxopalladate(II)
SP  - 69
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12964
ER  - 

@conference{
author = "Isaković, Anđelka and Jeremić, Marija and Krstić, Danijela and Čolović, Mirjana and Kortz, Ulrich and Misirlić-Denčić, Sonja",
year = "2021",
abstract = "Background: Polyoxometalates are a class of anionic, polynuclear metal-oxo clusters reported as promising in vitro and in vivo antitumor agents for several decades. The aim of this study was to investigate the antineuroblastoma potential of the polyoxopalladate(II) nanocube Na8[Pd13As8O34(OH)6]・42H2O (Pd13). Material and methods: All experiments were performed on human neuroblastoma cell line, SH-SY5Y. The number of viable cells after the treatment with Pd13 was assessed using an acid phosphatase viability assay. The level of superoxide ion, mitochondrial membrane potential, pan-caspase activity, acidic intracellular vesicles content, and the cell cycle was determined by flow cytometry. Results: The obtained results suggest that Pd13 caused a significant decrease in cell viability with IC50 values of 7.7 µM (24 h) and 4.4 µM (48 h). Pd13 induced depolarization of mitochondrial membrane (2 h), followed by ~ 30% increase in the production of the superoxide ion (O2-) 4 h after treatment. An increase (~ 30%) in pancaspase activation and disturbance of neuroblastma cell cycle were observed after 24 h treatment. Namely, Pd13 caused an increase (14.4%) in the number of cells with fragmented nuclear DNA (SubG0), a decrease (%) of cells in the G1 phase, and an increase (%) in the S phase, all suggestive of cell cycle arrest. Finally, Pd13 increased the orange to green fluorescence ratio for ~ 45% 24 h after treatment, supporting intracellular acidification. Conclusion: The polyoxopalladate, Pd13 can be regarded as a promising antineuroblastoma agent which induces oxidative stress, and causes pan-caspase activation, DNA fragmentation and cell cycle arrest, which are all hallmarks of apoptotic neuroblastoma cell death.",
publisher = "Belgrade : Serbian Association for Cancer Research (SDIR)",
journal = "SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts",
title = "Antineuroblastoma potential of polyoxopalladate(II)",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12964"
}

Isaković, A., Jeremić, M., Krstić, D., Čolović, M., Kortz, U.,& Misirlić-Denčić, S.. (2021). Antineuroblastoma potential of polyoxopalladate(II). in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
Belgrade : Serbian Association for Cancer Research (SDIR)., 69-69.
https://hdl.handle.net/21.15107/rcub_vinar_12964

Isaković A, Jeremić M, Krstić D, Čolović M, Kortz U, Misirlić-Denčić S. Antineuroblastoma potential of polyoxopalladate(II). in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts. 2021;:69-69.
https://hdl.handle.net/21.15107/rcub_vinar_12964 .

Isaković, Anđelka, Jeremić, Marija, Krstić, Danijela, Čolović, Mirjana, Kortz, Ulrich, Misirlić-Denčić, Sonja, "Antineuroblastoma potential of polyoxopalladate(II)" in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts (2021):69-69,
https://hdl.handle.net/21.15107/rcub_vinar_12964 .

TY  - CONF
AU  - Jeremić, Marija
AU  - Isaković, Anđelka
AU  - Krstić, Danijela
AU  - Čolović, Mirjana
AU  - Kortz, Ulrich
AU  - Misirlić-Denčić, Sonja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12965
AB  - Background: Polyoxo-noble-metalates, a class of molecular noble metal-oxo nanoclusters that combine features of both polyoxometalates and noble metals, are a promising platform for the development of nextgeneration antitumor metallodrugs. The aim of this study was to evaluate the antineuroblastoma potential of three novel polyoxopalladates. Material and methods: All experiments were performed on human neuroblastoma cell line, SHSY5Y. The three polyoxo-noble-palladates Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3] ・2NaOAc・32H2O (SrPd12), Na12[SnIVO8Pd12(PO4)8]·43H2O (SnPd12) and Na12[PbIVO8Pd12(PO4)8]·38H2O (PbPd12) were investigated in our study. The viability of neuroblastoma cells after 24h treatment was assessed using an acid phosphatase assay. The level of superoxide ion, mitochondrial membrane potential, pan-caspase activity, cell cycle analysis and acidic vesicles content were determined by flow cytometry using appropriate fluorochromes. Results: Calculated IC50 (μM; 24h) values were 75.8 ± 6.7 (SrPd12) and >>100 (SnPd12 and PbPd12), selecting SrPd12 as the most efficient. SrPd12 did not affect the mitochondrial membrane potential and superoxide production in neuroblastoma cells after short (2 h and 4 h) exposure. Also, it did not induce an increase in the number of neuroblastoma cells with fragmented DNA content, but displayed the cell cycle arrest: the ~ 23% reduction of neuroblastoma cells in G0/G1 phase and the ~ 17% increase in S phase. The treatment with SrPd12 did not increase the level of acidic vesicles but it increased the activity of caspases five-fold. Conclusion: Only SrPd12 exhibited a satisfactory antineuroblastoma action by inducing caspase activation and neuroblastoma cell cycle arrest.
PB  - Belgrade : Serbian Association for Cancer Research (SDIR)
C3  - SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
T1  - Selected polyoxopalladates as potential antitumor drug candidates
SP  - 70
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12965
ER  - 

@conference{
author = "Jeremić, Marija and Isaković, Anđelka and Krstić, Danijela and Čolović, Mirjana and Kortz, Ulrich and Misirlić-Denčić, Sonja",
year = "2021",
abstract = "Background: Polyoxo-noble-metalates, a class of molecular noble metal-oxo nanoclusters that combine features of both polyoxometalates and noble metals, are a promising platform for the development of nextgeneration antitumor metallodrugs. The aim of this study was to evaluate the antineuroblastoma potential of three novel polyoxopalladates. Material and methods: All experiments were performed on human neuroblastoma cell line, SHSY5Y. The three polyoxo-noble-palladates Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3] ・2NaOAc・32H2O (SrPd12), Na12[SnIVO8Pd12(PO4)8]·43H2O (SnPd12) and Na12[PbIVO8Pd12(PO4)8]·38H2O (PbPd12) were investigated in our study. The viability of neuroblastoma cells after 24h treatment was assessed using an acid phosphatase assay. The level of superoxide ion, mitochondrial membrane potential, pan-caspase activity, cell cycle analysis and acidic vesicles content were determined by flow cytometry using appropriate fluorochromes. Results: Calculated IC50 (μM; 24h) values were 75.8 ± 6.7 (SrPd12) and >>100 (SnPd12 and PbPd12), selecting SrPd12 as the most efficient. SrPd12 did not affect the mitochondrial membrane potential and superoxide production in neuroblastoma cells after short (2 h and 4 h) exposure. Also, it did not induce an increase in the number of neuroblastoma cells with fragmented DNA content, but displayed the cell cycle arrest: the ~ 23% reduction of neuroblastoma cells in G0/G1 phase and the ~ 17% increase in S phase. The treatment with SrPd12 did not increase the level of acidic vesicles but it increased the activity of caspases five-fold. Conclusion: Only SrPd12 exhibited a satisfactory antineuroblastoma action by inducing caspase activation and neuroblastoma cell cycle arrest.",
publisher = "Belgrade : Serbian Association for Cancer Research (SDIR)",
journal = "SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts",
title = "Selected polyoxopalladates as potential antitumor drug candidates",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12965"
}

Jeremić, M., Isaković, A., Krstić, D., Čolović, M., Kortz, U.,& Misirlić-Denčić, S.. (2021). Selected polyoxopalladates as potential antitumor drug candidates. in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
Belgrade : Serbian Association for Cancer Research (SDIR)., 70-70.
https://hdl.handle.net/21.15107/rcub_vinar_12965

Jeremić M, Isaković A, Krstić D, Čolović M, Kortz U, Misirlić-Denčić S. Selected polyoxopalladates as potential antitumor drug candidates. in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts. 2021;:70-70.
https://hdl.handle.net/21.15107/rcub_vinar_12965 .

Jeremić, Marija, Isaković, Anđelka, Krstić, Danijela, Čolović, Mirjana, Kortz, Ulrich, Misirlić-Denčić, Sonja, "Selected polyoxopalladates as potential antitumor drug candidates" in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts (2021):70-70,
https://hdl.handle.net/21.15107/rcub_vinar_12965 .

TY  - JOUR
AU  - Isaković, Anđelka
AU  - Čolović, Mirjana B.
AU  - Ma, Tian
AU  - Ma, Xiang
AU  - Jeremić, Marija
AU  - Gerić, Marko
AU  - Gajski, Goran
AU  - Misirlić-Denčić, Sonja
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9952
AB  - Polyoxo-noble-metalates (PONMs), a class of molecular noble metal-oxo nanoclusters that combine features of both polyoxometalates and noble metals, are a promising platform for the development of next-generation antitumor metallodrugs. This study aimed to evaluate the antitumor potential against human neuroblastoma cells (SH-SY5Y), as well as toxicity towards healthy human peripheral blood cells (HPBCs), of five polyoxopalladates(II): (Na8[Pd13As8O34(OH)6]·42H2O (Pd13), Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3]·2NaOAc·32H2O (SrPd12), Na6[Pd13(AsPh)8O32]·23H2O (Pd13L), Na12[SnO8Pd12(PO4)8]·43H2O (SnPd12), and Na12[PbO8Pd12(PO4)8]·38H2O (PbPd12)), as the largest subset of PONMs. A pure inorganic, Pd13, was found as the most potent and selective antineuroblastoma agent with IC50 values (µM) of 7.2 ± 2.2 and 4.4 ± 1.2 for 24 and 48 h treatment, respectively, even lower than cisplatin (28.4 ± 7.4 and 11.6 ± 0.8). The obtained IC50 values (µM) for 24/48 h treatment with SrPd12 and Pd13L were 75.8 ± 6.7/76.7 ± 22.9 and 63.8 ± 3.6/21.4 ± 10.8, respectively, whereas SnPd12 and PbPd12 did not remarkably affect the SH-SY5Y viability (IC50 > > 100 µM). Pd13 caused depolarisation of inner mitochondrial membrane prior to superoxide ion hyperproduction, followed by caspase activation, DNA fragmentation and cell cycle arrest, all hallmarks of apoptotic cell death, and accompanied by an increase in acidic vesicles content, suggestive of autophagy induction. Importantly, Pd13 demonstrated the antitumor effect at concentrations not cytogenotoxic for normal HPBCs. On the contrary, SrPd12 and Pd13L at concentrations ≥ 1/3 IC50 (24 h) decreased HPBC viability and increased % tail DNA up to 42% and 3.05 times, respectively, related to control. SnPd12 and PbPd12, previously confirmed promising antileukemic agents, did not exhibit cytogenotoxicity to HPBCs, and thus could be regarded as tumor cell specific and selective drug candidates.
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - Selected polyoxopalladates as promising and selective antitumor drug candidates
VL  - 26
IS  - 8
SP  - 957
EP  - 971
DO  - 10.1007/s00775-021-01905-4
ER  - 

@article{
author = "Isaković, Anđelka and Čolović, Mirjana B. and Ma, Tian and Ma, Xiang and Jeremić, Marija and Gerić, Marko and Gajski, Goran and Misirlić-Denčić, Sonja and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2021",
abstract = "Polyoxo-noble-metalates (PONMs), a class of molecular noble metal-oxo nanoclusters that combine features of both polyoxometalates and noble metals, are a promising platform for the development of next-generation antitumor metallodrugs. This study aimed to evaluate the antitumor potential against human neuroblastoma cells (SH-SY5Y), as well as toxicity towards healthy human peripheral blood cells (HPBCs), of five polyoxopalladates(II): (Na8[Pd13As8O34(OH)6]·42H2O (Pd13), Na4[SrPd12O6(OH)3(PhAsO3)6(OAc)3]·2NaOAc·32H2O (SrPd12), Na6[Pd13(AsPh)8O32]·23H2O (Pd13L), Na12[SnO8Pd12(PO4)8]·43H2O (SnPd12), and Na12[PbO8Pd12(PO4)8]·38H2O (PbPd12)), as the largest subset of PONMs. A pure inorganic, Pd13, was found as the most potent and selective antineuroblastoma agent with IC50 values (µM) of 7.2 ± 2.2 and 4.4 ± 1.2 for 24 and 48 h treatment, respectively, even lower than cisplatin (28.4 ± 7.4 and 11.6 ± 0.8). The obtained IC50 values (µM) for 24/48 h treatment with SrPd12 and Pd13L were 75.8 ± 6.7/76.7 ± 22.9 and 63.8 ± 3.6/21.4 ± 10.8, respectively, whereas SnPd12 and PbPd12 did not remarkably affect the SH-SY5Y viability (IC50 > > 100 µM). Pd13 caused depolarisation of inner mitochondrial membrane prior to superoxide ion hyperproduction, followed by caspase activation, DNA fragmentation and cell cycle arrest, all hallmarks of apoptotic cell death, and accompanied by an increase in acidic vesicles content, suggestive of autophagy induction. Importantly, Pd13 demonstrated the antitumor effect at concentrations not cytogenotoxic for normal HPBCs. On the contrary, SrPd12 and Pd13L at concentrations ≥ 1/3 IC50 (24 h) decreased HPBC viability and increased % tail DNA up to 42% and 3.05 times, respectively, related to control. SnPd12 and PbPd12, previously confirmed promising antileukemic agents, did not exhibit cytogenotoxicity to HPBCs, and thus could be regarded as tumor cell specific and selective drug candidates.",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "Selected polyoxopalladates as promising and selective antitumor drug candidates",
volume = "26",
number = "8",
pages = "957-971",
doi = "10.1007/s00775-021-01905-4"
}

Isaković, A., Čolović, M. B., Ma, T., Ma, X., Jeremić, M., Gerić, M., Gajski, G., Misirlić-Denčić, S., Kortz, U.,& Krstić, D. Z.. (2021). Selected polyoxopalladates as promising and selective antitumor drug candidates. in JBIC Journal of Biological Inorganic Chemistry, 26(8), 957-971.
https://doi.org/10.1007/s00775-021-01905-4

Isaković A, Čolović MB, Ma T, Ma X, Jeremić M, Gerić M, Gajski G, Misirlić-Denčić S, Kortz U, Krstić DZ. Selected polyoxopalladates as promising and selective antitumor drug candidates. in JBIC Journal of Biological Inorganic Chemistry. 2021;26(8):957-971.
doi:10.1007/s00775-021-01905-4 .

Isaković, Anđelka, Čolović, Mirjana B., Ma, Tian, Ma, Xiang, Jeremić, Marija, Gerić, Marko, Gajski, Goran, Misirlić-Denčić, Sonja, Kortz, Ulrich, Krstić, Danijela Z., "Selected polyoxopalladates as promising and selective antitumor drug candidates" in JBIC Journal of Biological Inorganic Chemistry, 26, no. 8 (2021):957-971,
https://doi.org/10.1007/s00775-021-01905-4 . .

TY  - JOUR
AU  - Yang, Peng
AU  - Ma, Tian
AU  - Lang, Zhongling
AU  - Misirlić-Denčić, Sonja
AU  - Isaković, Anđelka
AU  - Benyei, Attila
AU  - Čolović, Mirjana B.
AU  - Marković, Ivanka
AU  - Krstić, Danijela Z.
AU  - Poblet, Josep M.
AU  - Lin, Zhengguo
AU  - Kortz, Ulrich
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8499
AB  - The first two examples of polyoxopalladates(II) (POPs) containing tetravalent metal ion guests, [MO8Pd12(PO4)8]12- (M = SnIV, PbIV), have been prepared and structurally characterized in the solid state, solution, and gas phase. The interactions of the metal ion guests and the palladium-oxo shell were studied by theoretical calculations. The POPs were shown to possess anticancer activity by causing oxidative stress inducing caspase activation and consecutive apoptosis of leukemic cells.
T2  - Inorganic Chemistry
T1  - Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO8Pd12(PO4)8]12-(M=SnIV, PbIV)
VL  - 58
IS  - 17
SP  - 11294
EP  - 11299
DO  - 10.1021/acs.inorgchem.9b01129
ER  - 

@article{
author = "Yang, Peng and Ma, Tian and Lang, Zhongling and Misirlić-Denčić, Sonja and Isaković, Anđelka and Benyei, Attila and Čolović, Mirjana B. and Marković, Ivanka and Krstić, Danijela Z. and Poblet, Josep M. and Lin, Zhengguo and Kortz, Ulrich",
year = "2019",
abstract = "The first two examples of polyoxopalladates(II) (POPs) containing tetravalent metal ion guests, [MO8Pd12(PO4)8]12- (M = SnIV, PbIV), have been prepared and structurally characterized in the solid state, solution, and gas phase. The interactions of the metal ion guests and the palladium-oxo shell were studied by theoretical calculations. The POPs were shown to possess anticancer activity by causing oxidative stress inducing caspase activation and consecutive apoptosis of leukemic cells.",
journal = "Inorganic Chemistry",
title = "Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO8Pd12(PO4)8]12-(M=SnIV, PbIV)",
volume = "58",
number = "17",
pages = "11294-11299",
doi = "10.1021/acs.inorgchem.9b01129"
}

Yang, P., Ma, T., Lang, Z., Misirlić-Denčić, S., Isaković, A., Benyei, A., Čolović, M. B., Marković, I., Krstić, D. Z., Poblet, J. M., Lin, Z.,& Kortz, U.. (2019). Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO8Pd12(PO4)8]12-(M=SnIV, PbIV). in Inorganic Chemistry, 58(17), 11294-11299.
https://doi.org/10.1021/acs.inorgchem.9b01129

Yang P, Ma T, Lang Z, Misirlić-Denčić S, Isaković A, Benyei A, Čolović MB, Marković I, Krstić DZ, Poblet JM, Lin Z, Kortz U. Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO8Pd12(PO4)8]12-(M=SnIV, PbIV). in Inorganic Chemistry. 2019;58(17):11294-11299.
doi:10.1021/acs.inorgchem.9b01129 .

Yang, Peng, Ma, Tian, Lang, Zhongling, Misirlić-Denčić, Sonja, Isaković, Anđelka, Benyei, Attila, Čolović, Mirjana B., Marković, Ivanka, Krstić, Danijela Z., Poblet, Josep M., Lin, Zhengguo, Kortz, Ulrich, "Tetravalent Metal Ion Guests in Polyoxopalladate Chemistry: Synthesis and Anticancer Activity of [MO8Pd12(PO4)8]12-(M=SnIV, PbIV)" in Inorganic Chemistry, 58, no. 17 (2019):11294-11299,
https://doi.org/10.1021/acs.inorgchem.9b01129 . .

TY  - JOUR
AU  - Leovac, Vukadin M.
AU  - Bogdanović, Goran A.
AU  - Jovanović, Ljiljana S.
AU  - Joksović, Ljubinka G.
AU  - Marković, Violeta
AU  - Joksović, Milan D.
AU  - Misirlić-Denčić, Sonja
AU  - Isaković, Anđelka
AU  - Markovic, Ivanka
AU  - Heinemann, Frank W.
AU  - Trifunović, Srećko R.
AU  - Dalovic, Ivica
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4592
AB  - New polymeric copper(II) complexes with two tridentate ONS thiosemicarbazone ligands containing substituted pyrazolone moiety were synthesized and characterized by means of spectroscopic, electrochemical and crystallographic techniques. While both ligands exist as different tautomers in the solid state and DMSO-d(6) solution, Cu(II) ion coordinates the ligands from the same tautomeric form with square-pyramidal geometry around each Cu atom. In the crystal structures, the copper(II) complex cation forms polymeric chains {[Cu(L)Cl(+)]}(n) with a bridging chlorine atom. One of the complexes was found to have a significantly higher cytotoxic potential in comparison with cisplatin in inhibition of several cell lines (HL60, REH, C6, L929 and B16). The results obtained on the basis of flow cytometry indicated that apoptosis could be possible mechanism of cell death. (C) 2011 Elsevier Inc. All rights reserved.
T2  - Journal of Inorganic Biochemistry
T1  - Synthesis, characterization and antitumor activity of polymeric copper(II) complexes with thiosemicarbazones of 3-methyl-5-oxo-1-phenyl-3-pyrazolin-4-carboxaldehyde and 5-oxo-3-phenyl-3-pyrazolin-4-carboxaldehyde
VL  - 105
IS  - 11
SP  - 1413
EP  - 1421
DO  - 10.1016/j.jinorgbio.2011.07.021
ER  - 

@article{
author = "Leovac, Vukadin M. and Bogdanović, Goran A. and Jovanović, Ljiljana S. and Joksović, Ljubinka G. and Marković, Violeta and Joksović, Milan D. and Misirlić-Denčić, Sonja and Isaković, Anđelka and Markovic, Ivanka and Heinemann, Frank W. and Trifunović, Srećko R. and Dalovic, Ivica",
year = "2011",
abstract = "New polymeric copper(II) complexes with two tridentate ONS thiosemicarbazone ligands containing substituted pyrazolone moiety were synthesized and characterized by means of spectroscopic, electrochemical and crystallographic techniques. While both ligands exist as different tautomers in the solid state and DMSO-d(6) solution, Cu(II) ion coordinates the ligands from the same tautomeric form with square-pyramidal geometry around each Cu atom. In the crystal structures, the copper(II) complex cation forms polymeric chains {[Cu(L)Cl(+)]}(n) with a bridging chlorine atom. One of the complexes was found to have a significantly higher cytotoxic potential in comparison with cisplatin in inhibition of several cell lines (HL60, REH, C6, L929 and B16). The results obtained on the basis of flow cytometry indicated that apoptosis could be possible mechanism of cell death. (C) 2011 Elsevier Inc. All rights reserved.",
journal = "Journal of Inorganic Biochemistry",
title = "Synthesis, characterization and antitumor activity of polymeric copper(II) complexes with thiosemicarbazones of 3-methyl-5-oxo-1-phenyl-3-pyrazolin-4-carboxaldehyde and 5-oxo-3-phenyl-3-pyrazolin-4-carboxaldehyde",
volume = "105",
number = "11",
pages = "1413-1421",
doi = "10.1016/j.jinorgbio.2011.07.021"
}

Leovac, V. M., Bogdanović, G. A., Jovanović, L. S., Joksović, L. G., Marković, V., Joksović, M. D., Misirlić-Denčić, S., Isaković, A., Markovic, I., Heinemann, F. W., Trifunović, S. R.,& Dalovic, I.. (2011). Synthesis, characterization and antitumor activity of polymeric copper(II) complexes with thiosemicarbazones of 3-methyl-5-oxo-1-phenyl-3-pyrazolin-4-carboxaldehyde and 5-oxo-3-phenyl-3-pyrazolin-4-carboxaldehyde. in Journal of Inorganic Biochemistry, 105(11), 1413-1421.
https://doi.org/10.1016/j.jinorgbio.2011.07.021

Leovac VM, Bogdanović GA, Jovanović LS, Joksović LG, Marković V, Joksović MD, Misirlić-Denčić S, Isaković A, Markovic I, Heinemann FW, Trifunović SR, Dalovic I. Synthesis, characterization and antitumor activity of polymeric copper(II) complexes with thiosemicarbazones of 3-methyl-5-oxo-1-phenyl-3-pyrazolin-4-carboxaldehyde and 5-oxo-3-phenyl-3-pyrazolin-4-carboxaldehyde. in Journal of Inorganic Biochemistry. 2011;105(11):1413-1421.
doi:10.1016/j.jinorgbio.2011.07.021 .

Leovac, Vukadin M., Bogdanović, Goran A., Jovanović, Ljiljana S., Joksović, Ljubinka G., Marković, Violeta, Joksović, Milan D., Misirlić-Denčić, Sonja, Isaković, Anđelka, Markovic, Ivanka, Heinemann, Frank W., Trifunović, Srećko R., Dalovic, Ivica, "Synthesis, characterization and antitumor activity of polymeric copper(II) complexes with thiosemicarbazones of 3-methyl-5-oxo-1-phenyl-3-pyrazolin-4-carboxaldehyde and 5-oxo-3-phenyl-3-pyrazolin-4-carboxaldehyde" in Journal of Inorganic Biochemistry, 105, no. 11 (2011):1413-1421,
https://doi.org/10.1016/j.jinorgbio.2011.07.021 . .