TY  - JOUR
AU  - Đurašević, Siniša
AU  - Ružičić, Aleksandra
AU  - Lakić, Iva
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Glumac, Sofija
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Drakulić, Dunja R.
AU  - Stanković, Sanja
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10173
AB  - A dysregulated and overwhelming response to an infection accompanied by the exaggerated pro-inflammatory state and metabolism disturbance leads to the fatal outcome in sepsis. Previously we showed that meldonium, an anti-ischemic drug clinically used to treat myocardial and cerebral ischemia, strongly increases mortality in faecal-induced peritonitis (FIP) in rats. We postulated that the same mechanism that is responsible for the otherwise strong anti-inflammatory effects of meldonium could be the culprit of the increased mortality. In the present study, we applied the LPS-induced model of sepsis to explore the presence of any differences from and/or similarities to the FIP model. When it comes to energy production, despite some shared similarities, it is evident that LPS and FIP models of sepsis differ greatly. A different profile of sympathoadrenal activation may account for this observation, as it was lacking in the FIP model, whereas in the LPS model it was strong enough to overcome the effects of meldonium. Therefore, choosing the appropriate model of sepsis induction is of great importance, especially if energy homeostasis is the main focus of the study. Even when differences in the experimental design of the two models are acknowledged, the role of different patterns of energy production cannot be excluded. On that account, our results draw attention to the importance of uninterrupted energy production in sepsis but also call for much-needed revisions of the current recommendations for its treatment.
T2  - International Journal of Molecular Sciences
T1  - The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats
VL  - 23
IS  - 4
SP  - 2395
DO  - 10.3390/ijms23042395
ER  - 

@article{
author = "Đurašević, Siniša and Ružičić, Aleksandra and Lakić, Iva and Tosti, Tomislav and Đurović, Saša and Glumac, Sofija and Pejić, Snežana and Todorović, Ana and Drakulić, Dunja R. and Stanković, Sanja and Jasnić, Nebojša and Đorđević, Jelena and Todorović, Zoran",
year = "2022",
abstract = "A dysregulated and overwhelming response to an infection accompanied by the exaggerated pro-inflammatory state and metabolism disturbance leads to the fatal outcome in sepsis. Previously we showed that meldonium, an anti-ischemic drug clinically used to treat myocardial and cerebral ischemia, strongly increases mortality in faecal-induced peritonitis (FIP) in rats. We postulated that the same mechanism that is responsible for the otherwise strong anti-inflammatory effects of meldonium could be the culprit of the increased mortality. In the present study, we applied the LPS-induced model of sepsis to explore the presence of any differences from and/or similarities to the FIP model. When it comes to energy production, despite some shared similarities, it is evident that LPS and FIP models of sepsis differ greatly. A different profile of sympathoadrenal activation may account for this observation, as it was lacking in the FIP model, whereas in the LPS model it was strong enough to overcome the effects of meldonium. Therefore, choosing the appropriate model of sepsis induction is of great importance, especially if energy homeostasis is the main focus of the study. Even when differences in the experimental design of the two models are acknowledged, the role of different patterns of energy production cannot be excluded. On that account, our results draw attention to the importance of uninterrupted energy production in sepsis but also call for much-needed revisions of the current recommendations for its treatment.",
journal = "International Journal of Molecular Sciences",
title = "The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats",
volume = "23",
number = "4",
pages = "2395",
doi = "10.3390/ijms23042395"
}

Đurašević, S., Ružičić, A., Lakić, I., Tosti, T., Đurović, S., Glumac, S., Pejić, S., Todorović, A., Drakulić, D. R., Stanković, S., Jasnić, N., Đorđević, J.,& Todorović, Z.. (2022). The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats. in International Journal of Molecular Sciences, 23(4), 2395.
https://doi.org/10.3390/ijms23042395

Đurašević S, Ružičić A, Lakić I, Tosti T, Đurović S, Glumac S, Pejić S, Todorović A, Drakulić DR, Stanković S, Jasnić N, Đorđević J, Todorović Z. The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats. in International Journal of Molecular Sciences. 2022;23(4):2395.
doi:10.3390/ijms23042395 .

Đurašević, Siniša, Ružičić, Aleksandra, Lakić, Iva, Tosti, Tomislav, Đurović, Saša, Glumac, Sofija, Pejić, Snežana, Todorović, Ana, Drakulić, Dunja R., Stanković, Sanja, Jasnić, Nebojša, Đorđević, Jelena, Todorović, Zoran, "The Effects of a Meldonium Pre-Treatment on the Course of the LPS-Induced Sepsis in Rats" in International Journal of Molecular Sciences, 23, no. 4 (2022):2395,
https://doi.org/10.3390/ijms23042395 . .

TY  - JOUR
AU  - Vujović, Predrag
AU  - Lakić, Iva
AU  - Jasnić, Nebojša
AU  - Jevđović, Tanja
AU  - Đurašević, Siniša F.
AU  - Isenović, Esma R.
AU  - Đorđević, Jelena
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1120
AB  - Given that both prolactin and galanin take part in the regulation of energy homeostasis and that galanin is localized within lactotrophs, this study was aimed at comparing the pituitary expression patterns of prolactin and galanin during different phases of metabolic response to starvation in adult Wistar male rats. Food was removed at the onset of the dark phase (6:00 pm) and the animals were deprived for 6, 12, 24 and 48 h. Each of the starved groups (n=6) was killed simultaneously with a group of ad libitum-fed rats (n=6), and the intrapituitary levels of prolactin and galanin were examined. Galanin expression in the hypothalamus and the circulating levels of prolactin were also assessed. Starvation induced a rise in the intrapituitary prolactin level (p LT 0.001), whereas the opposite trend was detected in the serum (p LT 0.05). The galanin pituitary level was initially increased (6, 12 h) (p LT 0.05), but as starvation progressed, it first reached (at 24 h) and ultimately fell below the level recorded in the ad libitum rats (at 48 h) (p LT 0.05). Both prolactin and galanin were elevated in the hypothalamus after 24- and 48-h starvation. The results show that the starvation-induced increase in the pituitary prolactin expression did not lead to the rise in prolactin circulating levels, but rather resulted in the elevation of the prolactin hypothalamic content. Furthermore, the results suggest that under the circumstances of disturbed energy homeostasis, galanin might be responsible for the augmented prolactin production, initially at the pituitary and subsequently at the hypothalamic level.
T2  - Archives of Biological Sciences
T1  - Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern
VL  - 68
IS  - 1
SP  - 117
EP  - 123
DO  - 10.2298/ABS150525133V
ER  - 

@article{
author = "Vujović, Predrag and Lakić, Iva and Jasnić, Nebojša and Jevđović, Tanja and Đurašević, Siniša F. and Isenović, Esma R. and Đorđević, Jelena",
year = "2016",
abstract = "Given that both prolactin and galanin take part in the regulation of energy homeostasis and that galanin is localized within lactotrophs, this study was aimed at comparing the pituitary expression patterns of prolactin and galanin during different phases of metabolic response to starvation in adult Wistar male rats. Food was removed at the onset of the dark phase (6:00 pm) and the animals were deprived for 6, 12, 24 and 48 h. Each of the starved groups (n=6) was killed simultaneously with a group of ad libitum-fed rats (n=6), and the intrapituitary levels of prolactin and galanin were examined. Galanin expression in the hypothalamus and the circulating levels of prolactin were also assessed. Starvation induced a rise in the intrapituitary prolactin level (p LT 0.001), whereas the opposite trend was detected in the serum (p LT 0.05). The galanin pituitary level was initially increased (6, 12 h) (p LT 0.05), but as starvation progressed, it first reached (at 24 h) and ultimately fell below the level recorded in the ad libitum rats (at 48 h) (p LT 0.05). Both prolactin and galanin were elevated in the hypothalamus after 24- and 48-h starvation. The results show that the starvation-induced increase in the pituitary prolactin expression did not lead to the rise in prolactin circulating levels, but rather resulted in the elevation of the prolactin hypothalamic content. Furthermore, the results suggest that under the circumstances of disturbed energy homeostasis, galanin might be responsible for the augmented prolactin production, initially at the pituitary and subsequently at the hypothalamic level.",
journal = "Archives of Biological Sciences",
title = "Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern",
volume = "68",
number = "1",
pages = "117-123",
doi = "10.2298/ABS150525133V"
}

Vujović, P., Lakić, I., Jasnić, N., Jevđović, T., Đurašević, S. F., Isenović, E. R.,& Đorđević, J.. (2016). Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern. in Archives of Biological Sciences, 68(1), 117-123.
https://doi.org/10.2298/ABS150525133V

Vujović P, Lakić I, Jasnić N, Jevđović T, Đurašević SF, Isenović ER, Đorđević J. Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern. in Archives of Biological Sciences. 2016;68(1):117-123.
doi:10.2298/ABS150525133V .

Vujović, Predrag, Lakić, Iva, Jasnić, Nebojša, Jevđović, Tanja, Đurašević, Siniša F., Isenović, Esma R., Đorđević, Jelena, "Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern" in Archives of Biological Sciences, 68, no. 1 (2016):117-123,
https://doi.org/10.2298/ABS150525133V . .

TY  - JOUR
AU  - Cvijic, Gordana
AU  - Lakić, Iva
AU  - Vujovic, R.
AU  - Jasnic, N.
AU  - Djurasevic, S.
AU  - Dronjak, Slađana
AU  - Đorđević, Jelena D.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5603
AB  - The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chronic exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity.
T2  - Archives of Biological Sciences
T1  - Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity
VL  - 65
IS  - 3
SP  - 919
EP  - 927
DO  - 10.2298/ABS1303919C
ER  - 

@article{
author = "Cvijic, Gordana and Lakić, Iva and Vujovic, R. and Jasnic, N. and Djurasevic, S. and Dronjak, Slađana and Đorđević, Jelena D.",
year = "2013",
abstract = "The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chronic exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity.",
journal = "Archives of Biological Sciences",
title = "Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity",
volume = "65",
number = "3",
pages = "919-927",
doi = "10.2298/ABS1303919C"
}

Cvijic, G., Lakić, I., Vujovic, R., Jasnic, N., Djurasevic, S., Dronjak, S.,& Đorđević, J. D.. (2013). Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity. in Archives of Biological Sciences, 65(3), 919-927.
https://doi.org/10.2298/ABS1303919C

Cvijic G, Lakić I, Vujovic R, Jasnic N, Djurasevic S, Dronjak S, Đorđević JD. Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity. in Archives of Biological Sciences. 2013;65(3):919-927.
doi:10.2298/ABS1303919C .

Cvijic, Gordana, Lakić, Iva, Vujovic, R., Jasnic, N., Djurasevic, S., Dronjak, Slađana, Đorđević, Jelena D., "Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity" in Archives of Biological Sciences, 65, no. 3 (2013):919-927,
https://doi.org/10.2298/ABS1303919C . .

TY  - JOUR
AU  - Lakić, Iva
AU  - Drenca, Tamara
AU  - Đorđević, Jelena D.
AU  - Vujovic, P.
AU  - Jasnic, N.
AU  - Djurasevic, S.
AU  - Dronjak, Slađana
AU  - Cvijic, Gordana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4466
AB  - Interscapular brown adipose tissue (IBAT) is an energy storing organ involved in the maintenance of homeostasis in stress conditions when the balance of energy supplies is disturbed. The major regulator of IBAT activity is the sympathetic nervous system (SNS). Since genetic background is responsible for the individual differences in neuroendocrine stress responsivity, spontaneously hypertensive rats (SHR) that have a genetically increased general sympathetic output are a useful model for studying adaptive processes in stress conditions. Our aim was to test the effect of acute and/or chronic exposure to various stressors (thermal-cold, psychophysical-immobilization and psychosocial-isolation) on IBAT SNS and the metabolic activity in SHR, by measuring the number of monoamine-containing nerve endings and uncoupling protein-1 (UCP-1) content. The obtained results show that the IBAT SNS activity of unstressed SHR was stimulated by the administration of a single acute or chronic stressor and was independent of the duration or type of stressor, while chronic pre-stress of isolation suppressed further the SNS reaction to novel acute stress exposure. The IBAT UCP-1 content followed SNS changes, suggesting that this system is dominant in the regulation of IBAT metabolic rate in SHR.
T2  - Archives of Biological Sciences
T1  - Novel Acute Stressor Effects on Interscapular Brown Adipose Tissue Sympathetic Inervation and Ucp-1 Content in Chronically Isolated and Spontaneously Hypertensive Rats
VL  - 63
IS  - 3
SP  - 589
EP  - 596
DO  - 10.2298/ABS1103589L
ER  - 

@article{
author = "Lakić, Iva and Drenca, Tamara and Đorđević, Jelena D. and Vujovic, P. and Jasnic, N. and Djurasevic, S. and Dronjak, Slađana and Cvijic, Gordana",
year = "2011",
abstract = "Interscapular brown adipose tissue (IBAT) is an energy storing organ involved in the maintenance of homeostasis in stress conditions when the balance of energy supplies is disturbed. The major regulator of IBAT activity is the sympathetic nervous system (SNS). Since genetic background is responsible for the individual differences in neuroendocrine stress responsivity, spontaneously hypertensive rats (SHR) that have a genetically increased general sympathetic output are a useful model for studying adaptive processes in stress conditions. Our aim was to test the effect of acute and/or chronic exposure to various stressors (thermal-cold, psychophysical-immobilization and psychosocial-isolation) on IBAT SNS and the metabolic activity in SHR, by measuring the number of monoamine-containing nerve endings and uncoupling protein-1 (UCP-1) content. The obtained results show that the IBAT SNS activity of unstressed SHR was stimulated by the administration of a single acute or chronic stressor and was independent of the duration or type of stressor, while chronic pre-stress of isolation suppressed further the SNS reaction to novel acute stress exposure. The IBAT UCP-1 content followed SNS changes, suggesting that this system is dominant in the regulation of IBAT metabolic rate in SHR.",
journal = "Archives of Biological Sciences",
title = "Novel Acute Stressor Effects on Interscapular Brown Adipose Tissue Sympathetic Inervation and Ucp-1 Content in Chronically Isolated and Spontaneously Hypertensive Rats",
volume = "63",
number = "3",
pages = "589-596",
doi = "10.2298/ABS1103589L"
}

Lakić, I., Drenca, T., Đorđević, J. D., Vujovic, P., Jasnic, N., Djurasevic, S., Dronjak, S.,& Cvijic, G.. (2011). Novel Acute Stressor Effects on Interscapular Brown Adipose Tissue Sympathetic Inervation and Ucp-1 Content in Chronically Isolated and Spontaneously Hypertensive Rats. in Archives of Biological Sciences, 63(3), 589-596.
https://doi.org/10.2298/ABS1103589L

Lakić I, Drenca T, Đorđević JD, Vujovic P, Jasnic N, Djurasevic S, Dronjak S, Cvijic G. Novel Acute Stressor Effects on Interscapular Brown Adipose Tissue Sympathetic Inervation and Ucp-1 Content in Chronically Isolated and Spontaneously Hypertensive Rats. in Archives of Biological Sciences. 2011;63(3):589-596.
doi:10.2298/ABS1103589L .

Lakić, Iva, Drenca, Tamara, Đorđević, Jelena D., Vujovic, P., Jasnic, N., Djurasevic, S., Dronjak, Slađana, Cvijic, Gordana, "Novel Acute Stressor Effects on Interscapular Brown Adipose Tissue Sympathetic Inervation and Ucp-1 Content in Chronically Isolated and Spontaneously Hypertensive Rats" in Archives of Biological Sciences, 63, no. 3 (2011):589-596,
https://doi.org/10.2298/ABS1103589L . .