TY  - JOUR
AU  - Stanojlović, Miloš R.
AU  - Horvat, Anica
AU  - Guševac, Ivana
AU  - Grković, Ivana
AU  - Mitrović, Nataša Lj.
AU  - Buzadzic, I.
AU  - Drakulić, Dunja R.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5445
AB  - To study time-dependent and gender-specific intracellular and biochemical mechanisms that lead to neurodegeneration due to moderate but persistent reduction of cerebral blood flow, adult male and female Wistar rats were divided into two main groups controls that underwent sham operation and animals subjected to permanent bilateral occlusion of common carotid arteries. Animals were sacrificed 3, 7 or 90 days following the insult. Expression of several apoptotic proteins in synaptic fractions along with Fluoro-Jade B staining and DNA fragmentation assay were used to estimate the apoptotic processes and potential neurodegeneration in cerebral cortex. Data suggest a time-specific increase of Bax as well as time- and gender-associated down-regulation in protein expression of Bcl-2, up-regulation of procaspase 3, accompanied with increased cleavage of procaspase 3 and PARP in synaptic terminals. Furthermore, time- but not gender-specific neurodegeneration was observed. Our findings support the concept of time- and gender-associated response to permanent bilateral occlusion of common carotid arteries, which would enable better understanding of the mechanisms underlying cerebral hypoperfusion.
T2  - Folia Biologica
T1  - Time Course of Cerebral Hypoperfusion-Induced Neurodegenerative Changes in the Cortex of Male and Female Rats
VL  - 60
IS  - 3
SP  - 123
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5445
ER  - 

@article{
author = "Stanojlović, Miloš R. and Horvat, Anica and Guševac, Ivana and Grković, Ivana and Mitrović, Nataša Lj. and Buzadzic, I. and Drakulić, Dunja R.",
year = "2014",
abstract = "To study time-dependent and gender-specific intracellular and biochemical mechanisms that lead to neurodegeneration due to moderate but persistent reduction of cerebral blood flow, adult male and female Wistar rats were divided into two main groups controls that underwent sham operation and animals subjected to permanent bilateral occlusion of common carotid arteries. Animals were sacrificed 3, 7 or 90 days following the insult. Expression of several apoptotic proteins in synaptic fractions along with Fluoro-Jade B staining and DNA fragmentation assay were used to estimate the apoptotic processes and potential neurodegeneration in cerebral cortex. Data suggest a time-specific increase of Bax as well as time- and gender-associated down-regulation in protein expression of Bcl-2, up-regulation of procaspase 3, accompanied with increased cleavage of procaspase 3 and PARP in synaptic terminals. Furthermore, time- but not gender-specific neurodegeneration was observed. Our findings support the concept of time- and gender-associated response to permanent bilateral occlusion of common carotid arteries, which would enable better understanding of the mechanisms underlying cerebral hypoperfusion.",
journal = "Folia Biologica",
title = "Time Course of Cerebral Hypoperfusion-Induced Neurodegenerative Changes in the Cortex of Male and Female Rats",
volume = "60",
number = "3",
pages = "123-132",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5445"
}

Stanojlović, M. R., Horvat, A., Guševac, I., Grković, I., Mitrović, N. Lj., Buzadzic, I.,& Drakulić, D. R.. (2014). Time Course of Cerebral Hypoperfusion-Induced Neurodegenerative Changes in the Cortex of Male and Female Rats. in Folia Biologica, 60(3), 123-132.
https://hdl.handle.net/21.15107/rcub_vinar_5445

Stanojlović MR, Horvat A, Guševac I, Grković I, Mitrović NL, Buzadzic I, Drakulić DR. Time Course of Cerebral Hypoperfusion-Induced Neurodegenerative Changes in the Cortex of Male and Female Rats. in Folia Biologica. 2014;60(3):123-132.
https://hdl.handle.net/21.15107/rcub_vinar_5445 .

Stanojlović, Miloš R., Horvat, Anica, Guševac, Ivana, Grković, Ivana, Mitrović, Nataša Lj., Buzadzic, I., Drakulić, Dunja R., "Time Course of Cerebral Hypoperfusion-Induced Neurodegenerative Changes in the Cortex of Male and Female Rats" in Folia Biologica, 60, no. 3 (2014):123-132,
https://hdl.handle.net/21.15107/rcub_vinar_5445 .