TY  - JOUR
AU  - Filipović, Jelena G.
AU  - Joksić, Gordana
AU  - Vujić, Dragana
AU  - Joksić, Ivana
AU  - Mrasek, Kristin
AU  - Weise, Anja
AU  - Liehr, Thomas
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1239
AB  - Background: Fanconi anemia (FA) is a chromosomal instability syndrome characterized by increased frequency of chromosomal breakages, chromosomal radial figures and accelerated telomere shortening. In this work we performed detailed molecular-cytogenetic characterization of breakpoints in primary lymphocytes of FA-D2 patients in different stages of the disease using fluorescent in situ hybridization. Results: We found that chromosomal breakpoints co-localize on the molecular level with common fragile sites, whereas their distribution pattern depends on the severity of the disease. Telomere quantitative fluorescent in situ hybridization revealed that telomere fusions and radial figures, especially radials which involve telomere sequences are the consequence of critically shortened telomeres that increase with the disease progression and could be considered as a predictive parameter during the course of the disease. Sex chromosomes in FA cells are also involved in radial formation indicating that specific X chromosome regions share homology with autosomes and also could serve as repair templates in resolving DNA damage. Conclusions: FA-D2 chromosomal breakpoints co-localize with common fragile sites, but their distribution pattern depends on the disease stage. Telomere fusions and radials figures which involve telomere sequences are the consequence of shortened telomeres, increase with disease progression and could be of predictive value.
T2  - Molecular Cytogenetics
T1  - First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease
VL  - 9
DO  - 10.1186/s13039-016-0280-6
ER  - 

@article{
author = "Filipović, Jelena G. and Joksić, Gordana and Vujić, Dragana and Joksić, Ivana and Mrasek, Kristin and Weise, Anja and Liehr, Thomas",
year = "2016",
abstract = "Background: Fanconi anemia (FA) is a chromosomal instability syndrome characterized by increased frequency of chromosomal breakages, chromosomal radial figures and accelerated telomere shortening. In this work we performed detailed molecular-cytogenetic characterization of breakpoints in primary lymphocytes of FA-D2 patients in different stages of the disease using fluorescent in situ hybridization. Results: We found that chromosomal breakpoints co-localize on the molecular level with common fragile sites, whereas their distribution pattern depends on the severity of the disease. Telomere quantitative fluorescent in situ hybridization revealed that telomere fusions and radial figures, especially radials which involve telomere sequences are the consequence of critically shortened telomeres that increase with the disease progression and could be considered as a predictive parameter during the course of the disease. Sex chromosomes in FA cells are also involved in radial formation indicating that specific X chromosome regions share homology with autosomes and also could serve as repair templates in resolving DNA damage. Conclusions: FA-D2 chromosomal breakpoints co-localize with common fragile sites, but their distribution pattern depends on the disease stage. Telomere fusions and radials figures which involve telomere sequences are the consequence of shortened telomeres, increase with disease progression and could be of predictive value.",
journal = "Molecular Cytogenetics",
title = "First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease",
volume = "9",
doi = "10.1186/s13039-016-0280-6"
}

Filipović, J. G., Joksić, G., Vujić, D., Joksić, I., Mrasek, K., Weise, A.,& Liehr, T.. (2016). First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease. in Molecular Cytogenetics, 9.
https://doi.org/10.1186/s13039-016-0280-6

Filipović JG, Joksić G, Vujić D, Joksić I, Mrasek K, Weise A, Liehr T. First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease. in Molecular Cytogenetics. 2016;9.
doi:10.1186/s13039-016-0280-6 .

Filipović, Jelena G., Joksić, Gordana, Vujić, Dragana, Joksić, Ivana, Mrasek, Kristin, Weise, Anja, Liehr, Thomas, "First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease" in Molecular Cytogenetics, 9 (2016),
https://doi.org/10.1186/s13039-016-0280-6 . .