TY  - CONF
AU  - Dinčić, Marko
AU  - Todorović, Jasna
AU  - Čolović, Mirjana
AU  - Ćetković Milisavljević, Mila
AU  - Kravić Stevović, Tamara
AU  - Milosavljević, Aleksandra
AU  - Milinković, Neda
AU  - Kortz, Urlich
AU  - Krstić, Danijela
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12942
AB  - Zahvaljujući brojnim istraživanjima koja su ispitivala biološka svojstva strukturno različitih polioksometalata, došlo se do zapažanja da ova kompleksna neorganska jedinjenja, pored antimikrobnog i antitumorskog delovanja, mogu biti delotvorna u snižavanju hiperglikemije kod pacova sa eksperimentalno izazvanim dijabetesom. Stoga, cilj ove studije je bio da se ispitaju antidijabetički potencijal i mogući toksični efekti dva polioksovolframata:(NH4 )14[NaP5 W30O110]·31H2 O, {NaP5 W30} i K14[AgP5 W30O110]·22H2 O·6KCl, {AgP5 W30}. U cilju realizacije postavljenog cilja, korišćena su tri eksperimentalna modela: (1) antihiperglikemijska screening studija u kojoj je ispitivan uticaj jednokratne intraperitonealne primene {NaP5 W30} i {AgP5 W30} (5, 10 i 20 mg/kg) na snižavanje hiperglikemije kod dijabetičkih pacova, (2) akutna peroralna toksikološka studija koja je istraživala hepato- i nefrotoksične efekte odabranih heteropolivolframata kod zdravih pacova i (3) studija posvećena rasvetljavanju mogućih mehanizama antidijabetičkog delovanja heteropolivolframata. Rezultati screening studije su pokazali da su oba ispitivana heteropolivolframata efikasna u snižavanju hiperglikemije, s tim što se {NaP5 W30}, u odnosu na {AgP5 W30}, pokazao kao moćniji antihiperglikemijski agens. Rezultati biohemijskih parametara funkcije i patohistološka analiza jetre i bubrege korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije pokazuju da dvonedeljna primena {NaP5 W30} i {AgP5 W30} (20 mg/kg) izaziva blagi do umereni stepen hepato- i nefrotoksičnosti kod zdravih životinja. U poslednjem eksperimentalnom protokolu, pokazano je da tronedeljna peroralna primena {NaP5 W30} (20 mg/kg) povećava koncentraciju insulina u serumu dijabetičkih pacova, što može biti jedan od mehanizama njegovog antidijabetičkog delovanja. Takođe, pokazano je da {NaP5 W30} ispoljava hepato-, nefro-, kardio- i neuroprotektivno dejstvo kod dijabetičkih pacova, što je procenjeno na osnovu analize: (1) relativne mase organa, (2) biohemijskih parametara funkcije, (3) parametara oksidativnog stresa u homogenatu tkiva, (4) aktivnosti acetilholinesteraze, Na+ /K+-ATPaze i ecto-ATPaza u sinaptozomima i (5) patohistoloških promena u tkivima korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije. Stoga, {NaP5 W30} i {AgP5 W30} mogu se smatrati mogućim neinsulinskim lekovima-kandidatima u terapiji dijabetesa tipa 2, koji bi se podvrgli daljim pretkliničkim istraživanjima.
C3  - Medical Research : Medicinska istraživanja
T1  - Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera
VL  - 56
IS  - 4
SP  - 132
EP  - 133
DO  - 10.5937/medi56-47579
ER  - 

@conference{
author = "Dinčić, Marko and Todorović, Jasna and Čolović, Mirjana and Ćetković Milisavljević, Mila and Kravić Stevović, Tamara and Milosavljević, Aleksandra and Milinković, Neda and Kortz, Urlich and Krstić, Danijela",
year = "2023",
abstract = "Zahvaljujući brojnim istraživanjima koja su ispitivala biološka svojstva strukturno različitih polioksometalata, došlo se do zapažanja da ova kompleksna neorganska jedinjenja, pored antimikrobnog i antitumorskog delovanja, mogu biti delotvorna u snižavanju hiperglikemije kod pacova sa eksperimentalno izazvanim dijabetesom. Stoga, cilj ove studije je bio da se ispitaju antidijabetički potencijal i mogući toksični efekti dva polioksovolframata:(NH4 )14[NaP5 W30O110]·31H2 O, {NaP5 W30} i K14[AgP5 W30O110]·22H2 O·6KCl, {AgP5 W30}. U cilju realizacije postavljenog cilja, korišćena su tri eksperimentalna modela: (1) antihiperglikemijska screening studija u kojoj je ispitivan uticaj jednokratne intraperitonealne primene {NaP5 W30} i {AgP5 W30} (5, 10 i 20 mg/kg) na snižavanje hiperglikemije kod dijabetičkih pacova, (2) akutna peroralna toksikološka studija koja je istraživala hepato- i nefrotoksične efekte odabranih heteropolivolframata kod zdravih pacova i (3) studija posvećena rasvetljavanju mogućih mehanizama antidijabetičkog delovanja heteropolivolframata. Rezultati screening studije su pokazali da su oba ispitivana heteropolivolframata efikasna u snižavanju hiperglikemije, s tim što se {NaP5 W30}, u odnosu na {AgP5 W30}, pokazao kao moćniji antihiperglikemijski agens. Rezultati biohemijskih parametara funkcije i patohistološka analiza jetre i bubrege korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije pokazuju da dvonedeljna primena {NaP5 W30} i {AgP5 W30} (20 mg/kg) izaziva blagi do umereni stepen hepato- i nefrotoksičnosti kod zdravih životinja. U poslednjem eksperimentalnom protokolu, pokazano je da tronedeljna peroralna primena {NaP5 W30} (20 mg/kg) povećava koncentraciju insulina u serumu dijabetičkih pacova, što može biti jedan od mehanizama njegovog antidijabetičkog delovanja. Takođe, pokazano je da {NaP5 W30} ispoljava hepato-, nefro-, kardio- i neuroprotektivno dejstvo kod dijabetičkih pacova, što je procenjeno na osnovu analize: (1) relativne mase organa, (2) biohemijskih parametara funkcije, (3) parametara oksidativnog stresa u homogenatu tkiva, (4) aktivnosti acetilholinesteraze, Na+ /K+-ATPaze i ecto-ATPaza u sinaptozomima i (5) patohistoloških promena u tkivima korišćenjem konvencionalne svetlosne i transmisione elektronske mikroskopije. Stoga, {NaP5 W30} i {AgP5 W30} mogu se smatrati mogućim neinsulinskim lekovima-kandidatima u terapiji dijabetesa tipa 2, koji bi se podvrgli daljim pretkliničkim istraživanjima.",
journal = "Medical Research : Medicinska istraživanja",
title = "Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera",
volume = "56",
number = "4",
pages = "132-133",
doi = "10.5937/medi56-47579"
}

Dinčić, M., Todorović, J., Čolović, M., Ćetković Milisavljević, M., Kravić Stevović, T., Milosavljević, A., Milinković, N., Kortz, U.,& Krstić, D.. (2023). Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera. in Medical Research : Medicinska istraživanja, 56(4), 132-133.
https://doi.org/10.5937/medi56-47579

Dinčić M, Todorović J, Čolović M, Ćetković Milisavljević M, Kravić Stevović T, Milosavljević A, Milinković N, Kortz U, Krstić D. Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera. in Medical Research : Medicinska istraživanja. 2023;56(4):132-133.
doi:10.5937/medi56-47579 .

Dinčić, Marko, Todorović, Jasna, Čolović, Mirjana, Ćetković Milisavljević, Mila, Kravić Stevović, Tamara, Milosavljević, Aleksandra, Milinković, Neda, Kortz, Urlich, Krstić, Danijela, "Razvoj novih antidijabetičkih lekova na bazi polioksometalatnih nanoklastera" in Medical Research : Medicinska istraživanja, 56, no. 4 (2023):132-133,
https://doi.org/10.5937/medi56-47579 . .

TY  - JOUR
AU  - Todorović, Jasna
AU  - Dinčić, Marko
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Nešović-Ostojić, Jelena
AU  - Kovačević, Sanjin
AU  - Lopičić, Srđan
AU  - Spasić, Svetolik
AU  - Brkić, Predrag
AU  - Milovanović, Aleksandar
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10745
AB  - Background: Thyroid dysfunctions as well as sleep abnormalities are usually followed by neurological, psychiatric and/or behavioral disorders. On the other hand, changes in the brain adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) activities show significant importance in pathogenetic pathways in the evolution of numerous neuropsychiatric diseases. Methods: This study aimed to evaluate the in vivo simultaneous effects of hypothyroidism and paradoxical sleep deprivation for 72 h on synaptosomalATPases and AChE activities of whole rat brains. In order to induce hypothyroidism, 6-n-propyl-2-thiouracil was administrated in drinking water during 21 days. The modified multiple platform method was used to induce paradoxical sleep deprivation. The AChE and ATPases activities were measured using spectrophotometric methods. Results: Hypothyroidism significantly increased the activity of Na+/K+-ATPase compared to other groups, while at the same time significantly decreased AChE activity compared to the CT and SD groups. Paradoxical sleep deprivation significantly increased AChE activity compared to other groups. The simultaneous effect of hypothyroidism and sleep deprivation reduced the activity of all three enzymes (for Na+/K+-ATPase between HT/SD and HT group p < 0.0001, SD group p < 0.001,CT group p = 0.013; for ecto-ATPases between HT/SD and HT group p = 0.0034, SD group p = 0.0001, CT group p = 0.0007; for AChE between HT/SD and HT group p < 0.05, SD group p < 0.0001, CT group p < 0.0001). Conclusions: The effect of simultaneous existence of hypothyroidism and paradoxical sleep deprivation reduces the activity of the Na+/K+-ATPase, ecto-ATPases, and AChE, what is different from individual effect of hypothyroidism and paradoxical sleep deprivation itself. This knowledge could help in the choice of appropriate therapy in such condition. © 2023 Elsevier B.V.
T2  - Sleep Medicine
T1  - The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain
VL  - 105
SP  - 14
EP  - 20
DO  - 10.1016/j.sleep.2023.03.002
ER  - 

@article{
author = "Todorović, Jasna and Dinčić, Marko and Krstić, Danijela Z. and Čolović, Mirjana B. and Nešović-Ostojić, Jelena and Kovačević, Sanjin and Lopičić, Srđan and Spasić, Svetolik and Brkić, Predrag and Milovanović, Aleksandar",
year = "2023",
abstract = "Background: Thyroid dysfunctions as well as sleep abnormalities are usually followed by neurological, psychiatric and/or behavioral disorders. On the other hand, changes in the brain adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) activities show significant importance in pathogenetic pathways in the evolution of numerous neuropsychiatric diseases. Methods: This study aimed to evaluate the in vivo simultaneous effects of hypothyroidism and paradoxical sleep deprivation for 72 h on synaptosomalATPases and AChE activities of whole rat brains. In order to induce hypothyroidism, 6-n-propyl-2-thiouracil was administrated in drinking water during 21 days. The modified multiple platform method was used to induce paradoxical sleep deprivation. The AChE and ATPases activities were measured using spectrophotometric methods. Results: Hypothyroidism significantly increased the activity of Na+/K+-ATPase compared to other groups, while at the same time significantly decreased AChE activity compared to the CT and SD groups. Paradoxical sleep deprivation significantly increased AChE activity compared to other groups. The simultaneous effect of hypothyroidism and sleep deprivation reduced the activity of all three enzymes (for Na+/K+-ATPase between HT/SD and HT group p < 0.0001, SD group p < 0.001,CT group p = 0.013; for ecto-ATPases between HT/SD and HT group p = 0.0034, SD group p = 0.0001, CT group p = 0.0007; for AChE between HT/SD and HT group p < 0.05, SD group p < 0.0001, CT group p < 0.0001). Conclusions: The effect of simultaneous existence of hypothyroidism and paradoxical sleep deprivation reduces the activity of the Na+/K+-ATPase, ecto-ATPases, and AChE, what is different from individual effect of hypothyroidism and paradoxical sleep deprivation itself. This knowledge could help in the choice of appropriate therapy in such condition. © 2023 Elsevier B.V.",
journal = "Sleep Medicine",
title = "The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain",
volume = "105",
pages = "14-20",
doi = "10.1016/j.sleep.2023.03.002"
}

Todorović, J., Dinčić, M., Krstić, D. Z., Čolović, M. B., Nešović-Ostojić, J., Kovačević, S., Lopičić, S., Spasić, S., Brkić, P.,& Milovanović, A.. (2023). The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain. in Sleep Medicine, 105, 14-20.
https://doi.org/10.1016/j.sleep.2023.03.002

Todorović J, Dinčić M, Krstić DZ, Čolović MB, Nešović-Ostojić J, Kovačević S, Lopičić S, Spasić S, Brkić P, Milovanović A. The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain. in Sleep Medicine. 2023;105:14-20.
doi:10.1016/j.sleep.2023.03.002 .

Todorović, Jasna, Dinčić, Marko, Krstić, Danijela Z., Čolović, Mirjana B., Nešović-Ostojić, Jelena, Kovačević, Sanjin, Lopičić, Srđan, Spasić, Svetolik, Brkić, Predrag, Milovanović, Aleksandar, "The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain" in Sleep Medicine, 105 (2023):14-20,
https://doi.org/10.1016/j.sleep.2023.03.002 . .

TY  - JOUR
AU  - Dinčić, Marko
AU  - Čolović, Mirjana B.
AU  - Sarić Matutinović, Marija
AU  - Ćetković, Mila
AU  - Kravić-Stevović, Tamara K.
AU  - Mougharbel, Ali S.
AU  - Todorović, Jasna
AU  - Ignjatović, Svetlana
AU  - Radosavljević, Branimir
AU  - Milisavljević, Milan
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8479
AB  - In this study, thein vivohypoglycemic effect of a donut-shaped polyanion salt (NH4)14[Na@P5W30O110]$31H2O{NaP5W30} and its Ag-containing derivative K14[Ag@P5W30O110]$22H2O$6KCl {AgP5W30}, as wellas their hepatotoxicity and nephrotoxicity, was evaluated. In the screening hypoglycemic study,Wistaralbinorats with streptozotocin induced diabetes were treated intraperitoneally with three single doses (5,10, and 20 mg per kg per b.w.) of both investigated polyoxotungstates. The blood glucose levels,measured before and after 2, 4 and 6 h polyoxotungstate application, showed that both studiedcompounds induced the most pronounced and time dependent glucose lowering effects at the doses of20 mg kg1. Thus, daily doses of 20 mg kg1were administered toWistar albinorats orally for 14 days infurther toxicity examinations. The serum glucose concentration and biochemical parameters of kidneyand liver function, as well as a histopathological analysis of kidney and liver tissues were evaluated 14days after the polyoxotungstate administration. Both investigated compounds did not induce statisticallysignificant alterations of the serum glucose and uric acid concentrations, as well as some of the liverfunction markers (serum alanine and aspartate aminotransferases, and alkaline phosphatase activities).However, the significant decrease in serum total protein and albumin concentrations and the increase inbiochemical parameters of renal function–serum urea (up to 63.1%) and creatinine concentrations (upto 23.3%) were observed for both polyoxotungstates. In addition, the detected biochemical changeswere in accordance with kidney and liver histhopathological analysis. Accordingly, the hepatotoxic andnephrotoxic effects of these potential antidiabetic polyoxotungstates could be considered as mild.
T2  - RSC Advances
T1  - In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system
VL  - 10
IS  - 5
SP  - 2846
EP  - 2855
DO  - 10.1039/C9RA09790B
ER  - 

@article{
author = "Dinčić, Marko and Čolović, Mirjana B. and Sarić Matutinović, Marija and Ćetković, Mila and Kravić-Stevović, Tamara K. and Mougharbel, Ali S. and Todorović, Jasna and Ignjatović, Svetlana and Radosavljević, Branimir and Milisavljević, Milan and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2020",
abstract = "In this study, thein vivohypoglycemic effect of a donut-shaped polyanion salt (NH4)14[Na@P5W30O110]$31H2O{NaP5W30} and its Ag-containing derivative K14[Ag@P5W30O110]$22H2O$6KCl {AgP5W30}, as wellas their hepatotoxicity and nephrotoxicity, was evaluated. In the screening hypoglycemic study,Wistaralbinorats with streptozotocin induced diabetes were treated intraperitoneally with three single doses (5,10, and 20 mg per kg per b.w.) of both investigated polyoxotungstates. The blood glucose levels,measured before and after 2, 4 and 6 h polyoxotungstate application, showed that both studiedcompounds induced the most pronounced and time dependent glucose lowering effects at the doses of20 mg kg1. Thus, daily doses of 20 mg kg1were administered toWistar albinorats orally for 14 days infurther toxicity examinations. The serum glucose concentration and biochemical parameters of kidneyand liver function, as well as a histopathological analysis of kidney and liver tissues were evaluated 14days after the polyoxotungstate administration. Both investigated compounds did not induce statisticallysignificant alterations of the serum glucose and uric acid concentrations, as well as some of the liverfunction markers (serum alanine and aspartate aminotransferases, and alkaline phosphatase activities).However, the significant decrease in serum total protein and albumin concentrations and the increase inbiochemical parameters of renal function–serum urea (up to 63.1%) and creatinine concentrations (upto 23.3%) were observed for both polyoxotungstates. In addition, the detected biochemical changeswere in accordance with kidney and liver histhopathological analysis. Accordingly, the hepatotoxic andnephrotoxic effects of these potential antidiabetic polyoxotungstates could be considered as mild.",
journal = "RSC Advances",
title = "In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system",
volume = "10",
number = "5",
pages = "2846-2855",
doi = "10.1039/C9RA09790B"
}

Dinčić, M., Čolović, M. B., Sarić Matutinović, M., Ćetković, M., Kravić-Stevović, T. K., Mougharbel, A. S., Todorović, J., Ignjatović, S., Radosavljević, B., Milisavljević, M., Kortz, U.,& Krstić, D. Z.. (2020). In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system. in RSC Advances, 10(5), 2846-2855.
https://doi.org/10.1039/C9RA09790B

Dinčić M, Čolović MB, Sarić Matutinović M, Ćetković M, Kravić-Stevović TK, Mougharbel AS, Todorović J, Ignjatović S, Radosavljević B, Milisavljević M, Kortz U, Krstić DZ. In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system. in RSC Advances. 2020;10(5):2846-2855.
doi:10.1039/C9RA09790B .

Dinčić, Marko, Čolović, Mirjana B., Sarić Matutinović, Marija, Ćetković, Mila, Kravić-Stevović, Tamara K., Mougharbel, Ali S., Todorović, Jasna, Ignjatović, Svetlana, Radosavljević, Branimir, Milisavljević, Milan, Kortz, Ulrich, Krstić, Danijela Z., "In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system" in RSC Advances, 10, no. 5 (2020):2846-2855,
https://doi.org/10.1039/C9RA09790B . .

TY  - CONF
AU  - Dinčić, Marko
AU  - Sarić, Marija
AU  - Čolović, Mirjana
AU  - Todorović, Jasna
AU  - Ignjatović, Svetlana
AU  - Radosavljević, Branimir
AU  - Mougharbel, Ali S.
AU  - Kortz, Ulrich
AU  - Krstić, Danijela
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12962
AB  - Introduction. Polyoxometalates (POMs) are negatively charged inorganic aggregates that possess potential antibacterial, anticancer, antidiabetic and antiviral effects. Although toxicity evaluation of drug candidates is necessary, reports of relevant toxicological studies of these compounds are relatively rare.  Aims. Since our preliminary results demonstrated biological activities of the donut-shaped POM {NaP5W30} (POM1) and the Ag + -containing derivative POM {AgP5W30} (POM2), the aim of present study was to evaluate their toxicological effects in vivo, using Wistar albino rats as an experimental model.  Methods. Animals (n = 6 per group) were orally treated with investigated POMs in daily doses of 20 mg/kg body weight for 14 days when the rats were sacrificed and blood samples were collected. The biochemical markers of renal (serum concentrations of urea - SUr and creatinine - SCr) and liver function (serum concentrations of total protein–TP and albumin - Alb, serum activities of aspartate aminotransferase - AST and alanine transaminase - ALT) were determined spectrophotometrically.  Results. The POM1 and POM2 were induced statistically significant increasing of SUr (in: mmol/L) (7.95 ± 0.35 and 6.83 ± 0.26 vs. 4.97 ± 0.47; p < 0.001 and p < 0.01, respectively) and SCr (in: mmol/L) (41.34 ± 0.84 and 39.06 ± 1.07 vs. 32.27 ± 0.61; p < 0.001 and p < 0.001, respectively) compared to the control group. Also, investigated compounds induced statistically significant decreasing of TP (in: g/L) (60.16 ± 1.43 and 58.53 ± 0.81 vs. 67.86 ± 0.03; p < 0.001 and p < 0.001, respectively) and Alb (in: g/L) (29.34 ± 0.58 and 29.45 ± 0.81 vs. 34.89 ± 0.41; p < 0.001 and p < 0.001, respectively) compared to the control group. In contrast, there was no statistically significant difference in AST and ALT between the untreated and treated groups (p > 0.05).  Conclusion. Obtained results suggested that both investigated POMs induce kidney injury as well as synthetic dysfunction of liver. Thus, their potential clinical application would require a more complex toxicological study.
C3  - Pathophysiology
T1  - Toxicity evaluation of two biologically active polyoxotungstates
VL  - 25
IS  - 3
SP  - 243
EP  - 244
DO  - 10.1016/j.pathophys.2018.07.177
ER  - 

@conference{
author = "Dinčić, Marko and Sarić, Marija and Čolović, Mirjana and Todorović, Jasna and Ignjatović, Svetlana and Radosavljević, Branimir and Mougharbel, Ali S. and Kortz, Ulrich and Krstić, Danijela",
year = "2018",
abstract = "Introduction. Polyoxometalates (POMs) are negatively charged inorganic aggregates that possess potential antibacterial, anticancer, antidiabetic and antiviral effects. Although toxicity evaluation of drug candidates is necessary, reports of relevant toxicological studies of these compounds are relatively rare.  Aims. Since our preliminary results demonstrated biological activities of the donut-shaped POM {NaP5W30} (POM1) and the Ag + -containing derivative POM {AgP5W30} (POM2), the aim of present study was to evaluate their toxicological effects in vivo, using Wistar albino rats as an experimental model.  Methods. Animals (n = 6 per group) were orally treated with investigated POMs in daily doses of 20 mg/kg body weight for 14 days when the rats were sacrificed and blood samples were collected. The biochemical markers of renal (serum concentrations of urea - SUr and creatinine - SCr) and liver function (serum concentrations of total protein–TP and albumin - Alb, serum activities of aspartate aminotransferase - AST and alanine transaminase - ALT) were determined spectrophotometrically.  Results. The POM1 and POM2 were induced statistically significant increasing of SUr (in: mmol/L) (7.95 ± 0.35 and 6.83 ± 0.26 vs. 4.97 ± 0.47; p < 0.001 and p < 0.01, respectively) and SCr (in: mmol/L) (41.34 ± 0.84 and 39.06 ± 1.07 vs. 32.27 ± 0.61; p < 0.001 and p < 0.001, respectively) compared to the control group. Also, investigated compounds induced statistically significant decreasing of TP (in: g/L) (60.16 ± 1.43 and 58.53 ± 0.81 vs. 67.86 ± 0.03; p < 0.001 and p < 0.001, respectively) and Alb (in: g/L) (29.34 ± 0.58 and 29.45 ± 0.81 vs. 34.89 ± 0.41; p < 0.001 and p < 0.001, respectively) compared to the control group. In contrast, there was no statistically significant difference in AST and ALT between the untreated and treated groups (p > 0.05).  Conclusion. Obtained results suggested that both investigated POMs induce kidney injury as well as synthetic dysfunction of liver. Thus, their potential clinical application would require a more complex toxicological study.",
journal = "Pathophysiology",
title = "Toxicity evaluation of two biologically active polyoxotungstates",
volume = "25",
number = "3",
pages = "243-244",
doi = "10.1016/j.pathophys.2018.07.177"
}

Dinčić, M., Sarić, M., Čolović, M., Todorović, J., Ignjatović, S., Radosavljević, B., Mougharbel, A. S., Kortz, U.,& Krstić, D.. (2018). Toxicity evaluation of two biologically active polyoxotungstates. in Pathophysiology, 25(3), 243-244.
https://doi.org/10.1016/j.pathophys.2018.07.177

Dinčić M, Sarić M, Čolović M, Todorović J, Ignjatović S, Radosavljević B, Mougharbel AS, Kortz U, Krstić D. Toxicity evaluation of two biologically active polyoxotungstates. in Pathophysiology. 2018;25(3):243-244.
doi:10.1016/j.pathophys.2018.07.177 .

Dinčić, Marko, Sarić, Marija, Čolović, Mirjana, Todorović, Jasna, Ignjatović, Svetlana, Radosavljević, Branimir, Mougharbel, Ali S., Kortz, Ulrich, Krstić, Danijela, "Toxicity evaluation of two biologically active polyoxotungstates" in Pathophysiology, 25, no. 3 (2018):243-244,
https://doi.org/10.1016/j.pathophys.2018.07.177 . .

TY  - JOUR
AU  - Dinčić, Marko
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Nešović Ostojić, Jelena
AU  - Kovačević, Sanjin
AU  - De Luka, Silvio R.
AU  - Đorđević, Drago M.
AU  - Ćirković, Saša
AU  - Brkić, Predrag
AU  - Todorović, Jasna
PY  - 2018
UR  - https://www.tandfonline.com/doi/full/10.1080/09553002.2018.1518611
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7957
AB  - Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.
T2  - International Journal of Radiation Biology
T1  - Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field
VL  - 94
IS  - 11
SP  - 1062
EP  - 1071
DO  - 10.1080/09553002.2018.1518611
ER  - 

@article{
author = "Dinčić, Marko and Krstić, Danijela Z. and Čolović, Mirjana B. and Nešović Ostojić, Jelena and Kovačević, Sanjin and De Luka, Silvio R. and Đorđević, Drago M. and Ćirković, Saša and Brkić, Predrag and Todorović, Jasna",
year = "2018",
abstract = "Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.",
journal = "International Journal of Radiation Biology",
title = "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field",
volume = "94",
number = "11",
pages = "1062-1071",
doi = "10.1080/09553002.2018.1518611"
}

Dinčić, M., Krstić, D. Z., Čolović, M. B., Nešović Ostojić, J., Kovačević, S., De Luka, S. R., Đorđević, D. M., Ćirković, S., Brkić, P.,& Todorović, J.. (2018). Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. in International Journal of Radiation Biology, 94(11), 1062-1071.
https://doi.org/10.1080/09553002.2018.1518611

Dinčić M, Krstić DZ, Čolović MB, Nešović Ostojić J, Kovačević S, De Luka SR, Đorđević DM, Ćirković S, Brkić P, Todorović J. Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. in International Journal of Radiation Biology. 2018;94(11):1062-1071.
doi:10.1080/09553002.2018.1518611 .

Dinčić, Marko, Krstić, Danijela Z., Čolović, Mirjana B., Nešović Ostojić, Jelena, Kovačević, Sanjin, De Luka, Silvio R., Đorđević, Drago M., Ćirković, Saša, Brkić, Predrag, Todorović, Jasna, "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field" in International Journal of Radiation Biology, 94, no. 11 (2018):1062-1071,
https://doi.org/10.1080/09553002.2018.1518611 . .

TY  - CONF
AU  - Dinčić, Marko
AU  - Todorović, Jasna
AU  - Krstić, Danijela
AU  - Čolović, Mirjana
AU  - Nešović Ostojić, Jelena
AU  - Kovačević, Sanjin D.
AU  - Milovanović, Aleksandar P.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12979
AB  - Hipotireoidizam i deprivacija REM spavanja prepoznati su kao mogući faktori rizika za nastanak neurodegenerativnih oboljenja, pre svega Alchajmerove bolesti (AB). Enzimi acetilholinesteraza (AchE) i Na+ /K+ -ATPaza imaju ulogu u taloženju amiloidnih plakova, tako da je njihova ukupna aktivnost u mozgu obolelog od AB snižena. Cilj istraživanja bio je utvrđivanje mogućeg efekta hipotireoidizma i deprivacije REM faze spavanja na aktivnost AchE i Na+ /K+ -ATPaze. Koristili smo eksperimentalni model hipotireoidizma izazvan propiltiouracilom. Nakon uvođenja u hipotiroidizam, eksperimentalna grupa životinja (n = 12) podeljena je u dve podrupe. Životinje iz prve podgrupe (n = 6) boravile su 72 h na maloj platformi, koja ne dozvoljava REM spavanje, jer usled atonije životinja upada u vodu i budi se. Drugoj podgrupi životinja (n = 6) omogućeno je spavanje. Nakon 72 h, životinje su žrtvovane, i u sinaptozomalnoj frakciji mozga pacova određivana je aktivnost AchE i Na+ /K+ -ATPaze spektrofotometrijski. Rezultati pokazuju da hipotireoidizam dovodi do snižene aktivnosti AchE i Na+ /K+ -ATPaze u odnosu na kontrolu (p ≤ 0,01), dok zajednički efekat hipotireoidizma i deprivacije REM spavanja dovodi do snižene aktivnosti AchE i Na+ /K+ -ATPaze u odnosu na kontrolu (n = 6, p ≤ 0,01), ali ne u odnosu na hipotiroidizam (p > 0,05). Na osnovu dobijenih rezultata možemo zaključiti da hipotireoidizam smanjuje aktivnost enzima AchE i Na+ /K+ -ATPaze, a da deprivacija REM faze spavanja dodatno ne smanjuje aktivnost ovih enzima u hipotireoidizmu, tako je mogući način delovanja hipotireoidizma kao faktora rizika za nastanak AB upravo sniženje aktivnosti ovih enzima.
PB  - Zlatibor : Specijalna bolnica za bolesti štitaste žlezde i bolesti metabolizma
C3  - Medicinski glasnik
T1  - Efekat deprivacije REM faze spavanja na aktivnost acetilholinesteraze i NA+/K+-ATPaze sinaptozoma mozga pacova u hipotiroidizmu
VL  - 22
IS  - 66
SP  - 69
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12979
ER  - 

@conference{
author = "Dinčić, Marko and Todorović, Jasna and Krstić, Danijela and Čolović, Mirjana and Nešović Ostojić, Jelena and Kovačević, Sanjin D. and Milovanović, Aleksandar P.",
year = "2017",
abstract = "Hipotireoidizam i deprivacija REM spavanja prepoznati su kao mogući faktori rizika za nastanak neurodegenerativnih oboljenja, pre svega Alchajmerove bolesti (AB). Enzimi acetilholinesteraza (AchE) i Na+ /K+ -ATPaza imaju ulogu u taloženju amiloidnih plakova, tako da je njihova ukupna aktivnost u mozgu obolelog od AB snižena. Cilj istraživanja bio je utvrđivanje mogućeg efekta hipotireoidizma i deprivacije REM faze spavanja na aktivnost AchE i Na+ /K+ -ATPaze. Koristili smo eksperimentalni model hipotireoidizma izazvan propiltiouracilom. Nakon uvođenja u hipotiroidizam, eksperimentalna grupa životinja (n = 12) podeljena je u dve podrupe. Životinje iz prve podgrupe (n = 6) boravile su 72 h na maloj platformi, koja ne dozvoljava REM spavanje, jer usled atonije životinja upada u vodu i budi se. Drugoj podgrupi životinja (n = 6) omogućeno je spavanje. Nakon 72 h, životinje su žrtvovane, i u sinaptozomalnoj frakciji mozga pacova određivana je aktivnost AchE i Na+ /K+ -ATPaze spektrofotometrijski. Rezultati pokazuju da hipotireoidizam dovodi do snižene aktivnosti AchE i Na+ /K+ -ATPaze u odnosu na kontrolu (p ≤ 0,01), dok zajednički efekat hipotireoidizma i deprivacije REM spavanja dovodi do snižene aktivnosti AchE i Na+ /K+ -ATPaze u odnosu na kontrolu (n = 6, p ≤ 0,01), ali ne u odnosu na hipotiroidizam (p > 0,05). Na osnovu dobijenih rezultata možemo zaključiti da hipotireoidizam smanjuje aktivnost enzima AchE i Na+ /K+ -ATPaze, a da deprivacija REM faze spavanja dodatno ne smanjuje aktivnost ovih enzima u hipotireoidizmu, tako je mogući način delovanja hipotireoidizma kao faktora rizika za nastanak AB upravo sniženje aktivnosti ovih enzima.",
publisher = "Zlatibor : Specijalna bolnica za bolesti štitaste žlezde i bolesti metabolizma",
journal = "Medicinski glasnik",
title = "Efekat deprivacije REM faze spavanja na aktivnost acetilholinesteraze i NA+/K+-ATPaze sinaptozoma mozga pacova u hipotiroidizmu",
volume = "22",
number = "66",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12979"
}

Dinčić, M., Todorović, J., Krstić, D., Čolović, M., Nešović Ostojić, J., Kovačević, S. D.,& Milovanović, A. P.. (2017). Efekat deprivacije REM faze spavanja na aktivnost acetilholinesteraze i NA+/K+-ATPaze sinaptozoma mozga pacova u hipotiroidizmu. in Medicinski glasnik
Zlatibor : Specijalna bolnica za bolesti štitaste žlezde i bolesti metabolizma., 22(66), 69-69.
https://hdl.handle.net/21.15107/rcub_vinar_12979

Dinčić M, Todorović J, Krstić D, Čolović M, Nešović Ostojić J, Kovačević SD, Milovanović AP. Efekat deprivacije REM faze spavanja na aktivnost acetilholinesteraze i NA+/K+-ATPaze sinaptozoma mozga pacova u hipotiroidizmu. in Medicinski glasnik. 2017;22(66):69-69.
https://hdl.handle.net/21.15107/rcub_vinar_12979 .

Dinčić, Marko, Todorović, Jasna, Krstić, Danijela, Čolović, Mirjana, Nešović Ostojić, Jelena, Kovačević, Sanjin D., Milovanović, Aleksandar P., "Efekat deprivacije REM faze spavanja na aktivnost acetilholinesteraze i NA+/K+-ATPaze sinaptozoma mozga pacova u hipotiroidizmu" in Medicinski glasnik, 22, no. 66 (2017):69-69,
https://hdl.handle.net/21.15107/rcub_vinar_12979 .