TY  - CONF
AU  - Colic, M
AU  - Tomić, S.
AU  - Janjetović, Kristina D.
AU  - Mihajlovic, D
AU  - Milenković, M
AU  - Kravić-Stevović, Tamara K.
AU  - Marković, Zoran M.
AU  - Todorović-Marković, Biljana
AU  - Špitalsky, Zdenko
AU  - Mičušik, Matej
AU  - Vucevic, D
AU  - Trajković, Vladimir S.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7750
AB  - Graphene quantum dots (GQD) are atom-thick nanodimensional carbon, very attractive for the application in theranostics due to their excellent physico-chemical and biological properties. However, their immunoregulatory properties are insufficiently investigated, especially for human immune cells. We found that non-toxic doses of GQD inhibit the production of proinflammatory and T helper (Th)1 cytokines, and augment the production of anti-inflammatory and Th2 cytokines by human peripheral blood  mononuclear cells. While unable to affect purified T cells directly, GQD impaired the differentiation and functions of monocyte-derived dendritic cells (DC), lowering  their capacity to stimulate T cell proliferation, development of Th1 and Th17 cells, and T-cell mediated cytotoxicity. Additionally, GQD-treated DC potentiated Th2  polarization, and induced suppressive CD4+CD25hiFoxp3hi regulatory T cells. After internalization in a dynamin-independent, cholesterol-dependent manner, GQD lowered the ROS generation and nuclear translocation of NF-B in DC. The activity of mammalian target of rapamycin (mTOR) was reduced by GQD, which correlated with the increase in transcription of autophagy genes and autophagic flux in DC. Genetic suppression of autophagy impaired the pro-tolerogenic effects of GQD on DC. Our results suggest that GQD-triggered autophagy promotes tolerogenic functions in monocyte-derived DC, which could be beneficial in inflammatory T-cell mediated pathologies, but also harmful in GQD-based anti-cancer therapy.
C3  - European Journal of Immunology
T1  - Graphene quantum dots induce tolerogenic properties in dendritic cells via induction of autophagy
VL  - 48
IS  - Supplement 1
SP  - 180
EP  - 181
DO  - 10.1002/eji.201871000
ER  - 

@conference{
author = "Colic, M and Tomić, S. and Janjetović, Kristina D. and Mihajlovic, D and Milenković, M and Kravić-Stevović, Tamara K. and Marković, Zoran M. and Todorović-Marković, Biljana and Špitalsky, Zdenko and Mičušik, Matej and Vucevic, D and Trajković, Vladimir S.",
year = "2018",
abstract = "Graphene quantum dots (GQD) are atom-thick nanodimensional carbon, very attractive for the application in theranostics due to their excellent physico-chemical and biological properties. However, their immunoregulatory properties are insufficiently investigated, especially for human immune cells. We found that non-toxic doses of GQD inhibit the production of proinflammatory and T helper (Th)1 cytokines, and augment the production of anti-inflammatory and Th2 cytokines by human peripheral blood  mononuclear cells. While unable to affect purified T cells directly, GQD impaired the differentiation and functions of monocyte-derived dendritic cells (DC), lowering  their capacity to stimulate T cell proliferation, development of Th1 and Th17 cells, and T-cell mediated cytotoxicity. Additionally, GQD-treated DC potentiated Th2  polarization, and induced suppressive CD4+CD25hiFoxp3hi regulatory T cells. After internalization in a dynamin-independent, cholesterol-dependent manner, GQD lowered the ROS generation and nuclear translocation of NF-B in DC. The activity of mammalian target of rapamycin (mTOR) was reduced by GQD, which correlated with the increase in transcription of autophagy genes and autophagic flux in DC. Genetic suppression of autophagy impaired the pro-tolerogenic effects of GQD on DC. Our results suggest that GQD-triggered autophagy promotes tolerogenic functions in monocyte-derived DC, which could be beneficial in inflammatory T-cell mediated pathologies, but also harmful in GQD-based anti-cancer therapy.",
journal = "European Journal of Immunology",
title = "Graphene quantum dots induce tolerogenic properties in dendritic cells via induction of autophagy",
volume = "48",
number = "Supplement 1",
pages = "180-181",
doi = "10.1002/eji.201871000"
}

Colic, M., Tomić, S., Janjetović, K. D., Mihajlovic, D., Milenković, M., Kravić-Stevović, T. K., Marković, Z. M., Todorović-Marković, B., Špitalsky, Z., Mičušik, M., Vucevic, D.,& Trajković, V. S.. (2018). Graphene quantum dots induce tolerogenic properties in dendritic cells via induction of autophagy. in European Journal of Immunology, 48(Supplement 1), 180-181.
https://doi.org/10.1002/eji.201871000

Colic M, Tomić S, Janjetović KD, Mihajlovic D, Milenković M, Kravić-Stevović TK, Marković ZM, Todorović-Marković B, Špitalsky Z, Mičušik M, Vucevic D, Trajković VS. Graphene quantum dots induce tolerogenic properties in dendritic cells via induction of autophagy. in European Journal of Immunology. 2018;48(Supplement 1):180-181.
doi:10.1002/eji.201871000 .

Colic, M, Tomić, S., Janjetović, Kristina D., Mihajlovic, D, Milenković, M, Kravić-Stevović, Tamara K., Marković, Zoran M., Todorović-Marković, Biljana, Špitalsky, Zdenko, Mičušik, Matej, Vucevic, D, Trajković, Vladimir S., "Graphene quantum dots induce tolerogenic properties in dendritic cells via induction of autophagy" in European Journal of Immunology, 48, no. Supplement 1 (2018):180-181,
https://doi.org/10.1002/eji.201871000 . .

TY  - JOUR
AU  - Pinkney, T.
AU  - Gvozdenović, M.
AU  - Jovanović, Dragana J.
AU  - Lausevic, Z.
AU  - Cvetković, D.
AU  - Maravic, M.
AU  - Milovanović, Branislav
AU  - Stojakovic, N.
AU  - Tripkovic, I.
AU  - Mihajlovic, D.
AU  - Nestorović, M.
AU  - Pecic, V.
AU  - Petrović, D.
AU  - Stanojevic, G.
AU  - Barisic, G.
AU  - Dimitrijevic, I.
AU  - Krivokapic, Z.
AU  - Markovic, V.
AU  - Popović, Milan
AU  - Aleksić, A.
AU  - Dabic, D.
AU  - Kostic, I.
AU  - Milojkovic, A.
AU  - Perunicic, V.
AU  - Lukić, Dejan
AU  - Petrović, T.
AU  - Radovanovic, D.
AU  - Radovanovic, Z.
AU  - Cuk, V. M.
AU  - Cuk, V. V.
AU  - Kenic, M.
AU  - Kovacevic, B.
AU  - Krdzic, I.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1667
AB  - Aim The anastomosis technique used following right-sided colonic resection is widely variable and may affect patient outcome. This study aimed to assess the association between leak and anastomosis technique (stapled vs handsewn). Method This was a prospective, multicentre, international audit including patients undergoing elective or emergency right hemicolectomy or ileo-caecal resection operations over a 2-month period in early 2015. The primary outcome measure was the presence of anastomotic leak within 30 days of surgery, determined using a prespecified definition. Mixed effects logistic regression models were used to assess the association between leak and anastomosis method, adjusting for patient, disease and operative cofactors, with centre included as a random-effect variable. Results This study included 3208 patients, of whom 78.4% (n = 2515) underwent surgery for malignancy and 11.7% (n = 375) underwent surgery for Crohns disease. An anastomosis was performed in 94.8% (n = 3041) of patients, which was handsewn in 38.9% (n = 1183) and stapled in 61.1% (n = 1858). Patients undergoing hand-sewn anastomosis were more likely to be emergency admissions (20.5% handsewn vs 12.9% stapled) and to undergo open surgery (54.7% handsewn vs 36.6% stapled). The overall anastomotic leak rate was 8.1% (245/3041), which was similar following handsewn (7.4%) and stapled (8.5%) techniques (P = 0.3). After adjustment for cofactors, the odds of a leak were higher for stapled anastomosis (adjusted OR = 1.43; 95% CI: 1.04-1.95; P = 0.03). Conclusion Despite being used in lower-risk patients, stapled anastomosis was associated with an increased anastomotic leak rate in this observational study. Further research is needed to define patient groups in whom a stapled anastomosis is safe.
T2  - Colorectal Disease
T1  - Relationship between method of anastomosis and anastomotic failure after right hemicolectomy and ileo-caecal resection: an international snapshot audit
VL  - 19
IS  - 8
SP  - O296
EP  - O311
DO  - 10.1111/codi.13646
ER  - 

@article{
author = "Pinkney, T. and Gvozdenović, M. and Jovanović, Dragana J. and Lausevic, Z. and Cvetković, D. and Maravic, M. and Milovanović, Branislav and Stojakovic, N. and Tripkovic, I. and Mihajlovic, D. and Nestorović, M. and Pecic, V. and Petrović, D. and Stanojevic, G. and Barisic, G. and Dimitrijevic, I. and Krivokapic, Z. and Markovic, V. and Popović, Milan and Aleksić, A. and Dabic, D. and Kostic, I. and Milojkovic, A. and Perunicic, V. and Lukić, Dejan and Petrović, T. and Radovanovic, D. and Radovanovic, Z. and Cuk, V. M. and Cuk, V. V. and Kenic, M. and Kovacevic, B. and Krdzic, I.",
year = "2017",
abstract = "Aim The anastomosis technique used following right-sided colonic resection is widely variable and may affect patient outcome. This study aimed to assess the association between leak and anastomosis technique (stapled vs handsewn). Method This was a prospective, multicentre, international audit including patients undergoing elective or emergency right hemicolectomy or ileo-caecal resection operations over a 2-month period in early 2015. The primary outcome measure was the presence of anastomotic leak within 30 days of surgery, determined using a prespecified definition. Mixed effects logistic regression models were used to assess the association between leak and anastomosis method, adjusting for patient, disease and operative cofactors, with centre included as a random-effect variable. Results This study included 3208 patients, of whom 78.4% (n = 2515) underwent surgery for malignancy and 11.7% (n = 375) underwent surgery for Crohns disease. An anastomosis was performed in 94.8% (n = 3041) of patients, which was handsewn in 38.9% (n = 1183) and stapled in 61.1% (n = 1858). Patients undergoing hand-sewn anastomosis were more likely to be emergency admissions (20.5% handsewn vs 12.9% stapled) and to undergo open surgery (54.7% handsewn vs 36.6% stapled). The overall anastomotic leak rate was 8.1% (245/3041), which was similar following handsewn (7.4%) and stapled (8.5%) techniques (P = 0.3). After adjustment for cofactors, the odds of a leak were higher for stapled anastomosis (adjusted OR = 1.43; 95% CI: 1.04-1.95; P = 0.03). Conclusion Despite being used in lower-risk patients, stapled anastomosis was associated with an increased anastomotic leak rate in this observational study. Further research is needed to define patient groups in whom a stapled anastomosis is safe.",
journal = "Colorectal Disease",
title = "Relationship between method of anastomosis and anastomotic failure after right hemicolectomy and ileo-caecal resection: an international snapshot audit",
volume = "19",
number = "8",
pages = "O296-O311",
doi = "10.1111/codi.13646"
}

Pinkney, T., Gvozdenović, M., Jovanović, D. J., Lausevic, Z., Cvetković, D., Maravic, M., Milovanović, B., Stojakovic, N., Tripkovic, I., Mihajlovic, D., Nestorović, M., Pecic, V., Petrović, D., Stanojevic, G., Barisic, G., Dimitrijevic, I., Krivokapic, Z., Markovic, V., Popović, M., Aleksić, A., Dabic, D., Kostic, I., Milojkovic, A., Perunicic, V., Lukić, D., Petrović, T., Radovanovic, D., Radovanovic, Z., Cuk, V. M., Cuk, V. V., Kenic, M., Kovacevic, B.,& Krdzic, I.. (2017). Relationship between method of anastomosis and anastomotic failure after right hemicolectomy and ileo-caecal resection: an international snapshot audit. in Colorectal Disease, 19(8), O296-O311.
https://doi.org/10.1111/codi.13646

Pinkney T, Gvozdenović M, Jovanović DJ, Lausevic Z, Cvetković D, Maravic M, Milovanović B, Stojakovic N, Tripkovic I, Mihajlovic D, Nestorović M, Pecic V, Petrović D, Stanojevic G, Barisic G, Dimitrijevic I, Krivokapic Z, Markovic V, Popović M, Aleksić A, Dabic D, Kostic I, Milojkovic A, Perunicic V, Lukić D, Petrović T, Radovanovic D, Radovanovic Z, Cuk VM, Cuk VV, Kenic M, Kovacevic B, Krdzic I. Relationship between method of anastomosis and anastomotic failure after right hemicolectomy and ileo-caecal resection: an international snapshot audit. in Colorectal Disease. 2017;19(8):O296-O311.
doi:10.1111/codi.13646 .

Pinkney, T., Gvozdenović, M., Jovanović, Dragana J., Lausevic, Z., Cvetković, D., Maravic, M., Milovanović, Branislav, Stojakovic, N., Tripkovic, I., Mihajlovic, D., Nestorović, M., Pecic, V., Petrović, D., Stanojevic, G., Barisic, G., Dimitrijevic, I., Krivokapic, Z., Markovic, V., Popović, Milan, Aleksić, A., Dabic, D., Kostic, I., Milojkovic, A., Perunicic, V., Lukić, Dejan, Petrović, T., Radovanovic, D., Radovanovic, Z., Cuk, V. M., Cuk, V. V., Kenic, M., Kovacevic, B., Krdzic, I., "Relationship between method of anastomosis and anastomotic failure after right hemicolectomy and ileo-caecal resection: an international snapshot audit" in Colorectal Disease, 19, no. 8 (2017):O296-O311,
https://doi.org/10.1111/codi.13646 . .