TY  - JOUR
AU  - Micić, Milutin
AU  - Antonijević, Đorđe
AU  - Milutinović-Smiljanić, Sanja
AU  - Trišić, Dijana
AU  - Čolović, Božana M.
AU  - Kosanović, Dejana
AU  - Prokić, Bogomir Bolka
AU  - Vasić, Jugoslav
AU  - Živković, Slavoljub
AU  - Milašin, Jelena
AU  - Danilović, Vesna
AU  - Đurić, Marija P.
AU  - Jokanović, Vukoman R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8993
AB  - The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators-bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.
T2  - Biomedizinische Technik
T1  - Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction
VL  - 65
IS  - 4
SP  - 491
EP  - 505
DO  - 10.1515/bmt-2019-0218
ER  - 

@article{
author = "Micić, Milutin and Antonijević, Đorđe and Milutinović-Smiljanić, Sanja and Trišić, Dijana and Čolović, Božana M. and Kosanović, Dejana and Prokić, Bogomir Bolka and Vasić, Jugoslav and Živković, Slavoljub and Milašin, Jelena and Danilović, Vesna and Đurić, Marija P. and Jokanović, Vukoman R.",
year = "2020",
abstract = "The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators-bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.",
journal = "Biomedizinische Technik",
title = "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction",
volume = "65",
number = "4",
pages = "491-505",
doi = "10.1515/bmt-2019-0218"
}

Micić, M., Antonijević, Đ., Milutinović-Smiljanić, S., Trišić, D., Čolović, B. M., Kosanović, D., Prokić, B. B., Vasić, J., Živković, S., Milašin, J., Danilović, V., Đurić, M. P.,& Jokanović, V. R.. (2020). Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedizinische Technik, 65(4), 491-505.
https://doi.org/10.1515/bmt-2019-0218

Micić M, Antonijević Đ, Milutinović-Smiljanić S, Trišić D, Čolović BM, Kosanović D, Prokić BB, Vasić J, Živković S, Milašin J, Danilović V, Đurić MP, Jokanović VR. Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedizinische Technik. 2020;65(4):491-505.
doi:10.1515/bmt-2019-0218 .

Micić, Milutin, Antonijević, Đorđe, Milutinović-Smiljanić, Sanja, Trišić, Dijana, Čolović, Božana M., Kosanović, Dejana, Prokić, Bogomir Bolka, Vasić, Jugoslav, Živković, Slavoljub, Milašin, Jelena, Danilović, Vesna, Đurić, Marija P., Jokanović, Vukoman R., "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction" in Biomedizinische Technik, 65, no. 4 (2020):491-505,
https://doi.org/10.1515/bmt-2019-0218 . .

TY  - JOUR
AU  - Paraš, Smiljana
AU  - Trišić, Dijana
AU  - Mitrović Ajtić, Olivera
AU  - Prokić, Bogomir
AU  - Drobne, Damjana
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9008
AB  - Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.
T2  - Nanomaterials
T1  - Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats
VL  - 10
IS  - 5
SP  - 918
DO  - 10.3390/nano10050918
ER  - 

@article{
author = "Paraš, Smiljana and Trišić, Dijana and Mitrović Ajtić, Olivera and Prokić, Bogomir and Drobne, Damjana and Živković, Slavoljub and Jokanović, Vukoman R.",
year = "2020",
abstract = "Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.",
journal = "Nanomaterials",
title = "Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats",
volume = "10",
number = "5",
pages = "918",
doi = "10.3390/nano10050918"
}

Paraš, S., Trišić, D., Mitrović Ajtić, O., Prokić, B., Drobne, D., Živković, S.,& Jokanović, V. R.. (2020). Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats. in Nanomaterials, 10(5), 918.
https://doi.org/10.3390/nano10050918

Paraš S, Trišić D, Mitrović Ajtić O, Prokić B, Drobne D, Živković S, Jokanović VR. Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats. in Nanomaterials. 2020;10(5):918.
doi:10.3390/nano10050918 .

Paraš, Smiljana, Trišić, Dijana, Mitrović Ajtić, Olivera, Prokić, Bogomir, Drobne, Damjana, Živković, Slavoljub, Jokanović, Vukoman R., "Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats" in Nanomaterials, 10, no. 5 (2020):918,
https://doi.org/10.3390/nano10050918 . .

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Čolović, Božana M.
AU  - Vasilijić, Saša
AU  - Prokić, Bogomir
AU  - Pašalić, Snežana
AU  - Jokanović, Vukoman R.
AU  - Tepavčević, Zvezdana
AU  - Marković, Dejan
PY  - 2018
UR  - http://link.springer.com/10.1007/s10856-018-6200-z
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7990
AB  - The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA+ using in vitro and in vivo model. Morphology of materials’ samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials’ suspensions were conducted by pH-meter. The cytotoxicity of materials’ solutions was tested by MTT test (100, 50, 25 and 12.5 mg/ml). LDH and 3H-thymidine assay were utilized for biocompatibility investigations of materials’ eluates (24 h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio ~2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA1 and ALBO-MPCA2 were high alkaline, while in case of MTA+ they were lower and continuously declined (p < 0.05). Investigated materials didn’t exhibit dose-dependent effect on metabolic activity of L929 cells (p > 0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24 h and 7 day were noticed (p < 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA1 and ALBO-MPCA2 after 7 day (p < 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p > 0.05). Evaluation of biocompatibility of both ALBO-MPCA1 and ALBO-MPCA2 indicate their potential clinical use. [Figure not available: see fulltext.]. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Journal of Materials Science: Materials in Medicine
T1  - Nanostructured endodontic materials mixed with different radiocontrast agents — biocompatibility study
VL  - 29
IS  - 12
SP  - 190
DO  - 10.1007/s10856-018-6200-z
ER  - 

@article{
author = "Ćetenović, Bojana and Čolović, Božana M. and Vasilijić, Saša and Prokić, Bogomir and Pašalić, Snežana and Jokanović, Vukoman R. and Tepavčević, Zvezdana and Marković, Dejan",
year = "2018",
abstract = "The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA+ using in vitro and in vivo model. Morphology of materials’ samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials’ suspensions were conducted by pH-meter. The cytotoxicity of materials’ solutions was tested by MTT test (100, 50, 25 and 12.5 mg/ml). LDH and 3H-thymidine assay were utilized for biocompatibility investigations of materials’ eluates (24 h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio ~2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA1 and ALBO-MPCA2 were high alkaline, while in case of MTA+ they were lower and continuously declined (p < 0.05). Investigated materials didn’t exhibit dose-dependent effect on metabolic activity of L929 cells (p > 0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24 h and 7 day were noticed (p < 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA1 and ALBO-MPCA2 after 7 day (p < 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p > 0.05). Evaluation of biocompatibility of both ALBO-MPCA1 and ALBO-MPCA2 indicate their potential clinical use. [Figure not available: see fulltext.]. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Journal of Materials Science: Materials in Medicine",
title = "Nanostructured endodontic materials mixed with different radiocontrast agents — biocompatibility study",
volume = "29",
number = "12",
pages = "190",
doi = "10.1007/s10856-018-6200-z"
}

Ćetenović, B., Čolović, B. M., Vasilijić, S., Prokić, B., Pašalić, S., Jokanović, V. R., Tepavčević, Z.,& Marković, D.. (2018). Nanostructured endodontic materials mixed with different radiocontrast agents — biocompatibility study. in Journal of Materials Science: Materials in Medicine, 29(12), 190.
https://doi.org/10.1007/s10856-018-6200-z

Ćetenović B, Čolović BM, Vasilijić S, Prokić B, Pašalić S, Jokanović VR, Tepavčević Z, Marković D. Nanostructured endodontic materials mixed with different radiocontrast agents — biocompatibility study. in Journal of Materials Science: Materials in Medicine. 2018;29(12):190.
doi:10.1007/s10856-018-6200-z .

Ćetenović, Bojana, Čolović, Božana M., Vasilijić, Saša, Prokić, Bogomir, Pašalić, Snežana, Jokanović, Vukoman R., Tepavčević, Zvezdana, Marković, Dejan, "Nanostructured endodontic materials mixed with different radiocontrast agents — biocompatibility study" in Journal of Materials Science: Materials in Medicine, 29, no. 12 (2018):190,
https://doi.org/10.1007/s10856-018-6200-z . .

TY  - JOUR
AU  - Jokanović, Vukoman R.
AU  - Čolović, Božana M.
AU  - Prokić, Bogomir Bolka
AU  - Tomanović, Nada
AU  - Popović Bajić, Marijana
AU  - Živković, Slavoljub
PY  - 2018
UR  - https://www.hindawi.com/journals/amse/2018/8493439/
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8061
AB  - As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in many in vitro and in vivo studies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.
T2  - Advances in Materials Science and Engineering
T1  - Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates
VL  - 2018
SP  - 1
EP  - 6
DO  - 10.1155/2018/8493439
ER  - 

@article{
author = "Jokanović, Vukoman R. and Čolović, Božana M. and Prokić, Bogomir Bolka and Tomanović, Nada and Popović Bajić, Marijana and Živković, Slavoljub",
year = "2018",
abstract = "As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in many in vitro and in vivo studies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.",
journal = "Advances in Materials Science and Engineering",
title = "Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates",
volume = "2018",
pages = "1-6",
doi = "10.1155/2018/8493439"
}

Jokanović, V. R., Čolović, B. M., Prokić, B. B., Tomanović, N., Popović Bajić, M.,& Živković, S.. (2018). Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates. in Advances in Materials Science and Engineering, 2018, 1-6.
https://doi.org/10.1155/2018/8493439

Jokanović VR, Čolović BM, Prokić BB, Tomanović N, Popović Bajić M, Živković S. Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates. in Advances in Materials Science and Engineering. 2018;2018:1-6.
doi:10.1155/2018/8493439 .

Jokanović, Vukoman R., Čolović, Božana M., Prokić, Bogomir Bolka, Tomanović, Nada, Popović Bajić, Marijana, Živković, Slavoljub, "Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates" in Advances in Materials Science and Engineering, 2018 (2018):1-6,
https://doi.org/10.1155/2018/8493439 . .