TY  - CONF
AU  - Mitrović, Nataša
AU  - Zarić, Marina
AU  - Martinović, Jelena
AU  - Guševac Stojanović, Ivana
AU  - Petrović, Snježana
AU  - Paunović, Marija
AU  - Vučić, Vesna
AU  - Grković, Ivana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11050
AB  - It is increasingly apparent that the prevention/treatment of neurodegenerative disorders is not only achieved through pharmacological therapy but also through the consumption of natural products. Flaxseed oil (or linseed oil, FSO) derived from the seeds of the flax (Linum usitatissimum L.) gained worldwide awareness as a neuroprotective agent due to its high content of omega-3 polyunsaturated fatty acids (n-3 PUFAs). Thus, the aim of this study was to examine the preventive effects of dietary FSO in trimethyltin (TMT) - induced hippocampal neurodegeneration and gliosis in female Wistar rats. Animals were continuously treated with FSO (1 ml/kg, orally) for two weeks, then received a single dose of TMT (8 mg/kg, i.p.), and application of FSO continued for twenty-one days. Data have convincingly shown that FSO continuous treatment ameliorated TMT-induced neuronal loss in the CA3 hippocampal region and ameliorated astrogliosis and microgliosis. FSO treatment elevated all tested n-3 fatty acids in the hippocampus: α-linolenic acid (ALA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), and consequently increased total amount of n-3 PUFA. However, no changes in n-6 fatty acids due to FSO treatment were observed. Consequently, FSO lowered n-6/n-3 ratio compared to TMT, having a protective effect on fatty acid profile in hippocampus. These findings support beneficial neuroprotective properties of FSO against TMT-induced model of neurodegeneration and hint at a promising preventive use of FSO in hippocampal degeneration and dysfunction
PB  - Belgrade : Serbian Neurocardiological Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11050
ER  - 

@conference{
author = "Mitrović, Nataša and Zarić, Marina and Martinović, Jelena and Guševac Stojanović, Ivana and Petrović, Snježana and Paunović, Marija and Vučić, Vesna and Grković, Ivana",
year = "2023",
abstract = "It is increasingly apparent that the prevention/treatment of neurodegenerative disorders is not only achieved through pharmacological therapy but also through the consumption of natural products. Flaxseed oil (or linseed oil, FSO) derived from the seeds of the flax (Linum usitatissimum L.) gained worldwide awareness as a neuroprotective agent due to its high content of omega-3 polyunsaturated fatty acids (n-3 PUFAs). Thus, the aim of this study was to examine the preventive effects of dietary FSO in trimethyltin (TMT) - induced hippocampal neurodegeneration and gliosis in female Wistar rats. Animals were continuously treated with FSO (1 ml/kg, orally) for two weeks, then received a single dose of TMT (8 mg/kg, i.p.), and application of FSO continued for twenty-one days. Data have convincingly shown that FSO continuous treatment ameliorated TMT-induced neuronal loss in the CA3 hippocampal region and ameliorated astrogliosis and microgliosis. FSO treatment elevated all tested n-3 fatty acids in the hippocampus: α-linolenic acid (ALA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), and consequently increased total amount of n-3 PUFA. However, no changes in n-6 fatty acids due to FSO treatment were observed. Consequently, FSO lowered n-6/n-3 ratio compared to TMT, having a protective effect on fatty acid profile in hippocampus. These findings support beneficial neuroprotective properties of FSO against TMT-induced model of neurodegeneration and hint at a promising preventive use of FSO in hippocampal degeneration and dysfunction",
publisher = "Belgrade : Serbian Neurocardiological Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11050"
}

Mitrović, N., Zarić, M., Martinović, J., Guševac Stojanović, I., Petrović, S., Paunović, M., Vučić, V.,& Grković, I.. (2023). Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neurocardiological Society., 81.
https://hdl.handle.net/21.15107/rcub_vinar_11050

Mitrović N, Zarić M, Martinović J, Guševac Stojanović I, Petrović S, Paunović M, Vučić V, Grković I. Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:81.
https://hdl.handle.net/21.15107/rcub_vinar_11050 .

Mitrović, Nataša, Zarić, Marina, Martinović, Jelena, Guševac Stojanović, Ivana, Petrović, Snježana, Paunović, Marija, Vučić, Vesna, Grković, Ivana, "Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):81,
https://hdl.handle.net/21.15107/rcub_vinar_11050 .

TY  - JOUR
AU  - Masnikosa, Romana
AU  - Pirić, David
AU  - Post, Julia Maria
AU  - Cvetković, Zorica
AU  - Petrović, Snježana
AU  - Paunović, Marija
AU  - Vučić, Vesna
AU  - Bindila, Laura
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11437
AB  - Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer.
T2  - Cancers
T1  - Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study
VL  - 15
IS  - 14
SP  - 3653
DO  - 10.3390/cancers15143653
ER  - 

@article{
author = "Masnikosa, Romana and Pirić, David and Post, Julia Maria and Cvetković, Zorica and Petrović, Snježana and Paunović, Marija and Vučić, Vesna and Bindila, Laura",
year = "2023",
abstract = "Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer.",
journal = "Cancers",
title = "Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study",
volume = "15",
number = "14",
pages = "3653",
doi = "10.3390/cancers15143653"
}

Masnikosa, R., Pirić, D., Post, J. M., Cvetković, Z., Petrović, S., Paunović, M., Vučić, V.,& Bindila, L.. (2023). Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study. in Cancers, 15(14), 3653.
https://doi.org/10.3390/cancers15143653

Masnikosa R, Pirić D, Post JM, Cvetković Z, Petrović S, Paunović M, Vučić V, Bindila L. Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study. in Cancers. 2023;15(14):3653.
doi:10.3390/cancers15143653 .

Masnikosa, Romana, Pirić, David, Post, Julia Maria, Cvetković, Zorica, Petrović, Snježana, Paunović, Marija, Vučić, Vesna, Bindila, Laura, "Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study" in Cancers, 15, no. 14 (2023):3653,
https://doi.org/10.3390/cancers15143653 . .

TY  - CONF
AU  - Stanojević, Ivana
AU  - Milošević, Maja
AU  - Petrović, Snježana
AU  - Drakulić, Dunja R.
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9324
AB  - Kinetic properties of ecto-nucleoside triphosphate diphosphohydrolase (NTPDases)
in synaptic terminals from whole rat brain were characterised. Three different
NTPDases are present in nervous system and hydrolysing ATP and ADP with
different affinities. ATP and ADP hydrolysis exhibited kinetic properties typical
for all members of the NTPDase, low substrate specificity for tri- and
diphosphonucleosides, divalent cation dependency and insensitivity towards iontransporting
ATPase inhibitors. Kinetic properties of ATPase and ADPase
component of NTPDases depend on postnatal developmental stages of rats, and
indicated different NTPDase family members present in various ontogeny stages.
Lowering in the NTPDases activities could result in a decreased production of
adenosine and change in extracellular ATP/adenosine ratio.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Kinetic properties of ntpdases in rat brain synaptic plasma membranes
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9324
ER  - 

@conference{
author = "Stanojević, Ivana and Milošević, Maja and Petrović, Snježana and Drakulić, Dunja R. and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "Kinetic properties of ecto-nucleoside triphosphate diphosphohydrolase (NTPDases)
in synaptic terminals from whole rat brain were characterised. Three different
NTPDases are present in nervous system and hydrolysing ATP and ADP with
different affinities. ATP and ADP hydrolysis exhibited kinetic properties typical
for all members of the NTPDase, low substrate specificity for tri- and
diphosphonucleosides, divalent cation dependency and insensitivity towards iontransporting
ATPase inhibitors. Kinetic properties of ATPase and ADPase
component of NTPDases depend on postnatal developmental stages of rats, and
indicated different NTPDase family members present in various ontogeny stages.
Lowering in the NTPDases activities could result in a decreased production of
adenosine and change in extracellular ATP/adenosine ratio.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Kinetic properties of ntpdases in rat brain synaptic plasma membranes",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9324"
}

Stanojević, I., Milošević, M., Petrović, S., Drakulić, D. R., Veličković, N.,& Horvat, A.. (2010). Kinetic properties of ntpdases in rat brain synaptic plasma membranes. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9324

Stanojević I, Milošević M, Petrović S, Drakulić DR, Veličković N, Horvat A. Kinetic properties of ntpdases in rat brain synaptic plasma membranes. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9324 .

Stanojević, Ivana, Milošević, Maja, Petrović, Snježana, Drakulić, Dunja R., Veličković, Nataša, Horvat, Anica, "Kinetic properties of ntpdases in rat brain synaptic plasma membranes" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9324 .

TY  - CONF
AU  - Petrović, S.
AU  - Milošević, Maja
AU  - Horvat, Anica
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9368
AB  - In the present study the modulation of Ca2+ ion flux in the synaptosomal mitochondria isolated from the ovariectyomized rat Brain Steam and the possible roll of membrane bound estradiol was examined. Physiological concentrations of 17β-estradiol binds specifically to isolated mitochondria (Vmax 3.37± 0.25 pmol/mg protein, Km 1.85± 0.06 nmol/l of free estradiol). Addition of 17β-estradiol (10 pmol/l - 1 nmol/ l) in vitro decreased mitochondrial calcium ion efflux significantly (25%) after 10 minutes. Modulation of calcium ion efflux and mitochondrial ion retention may be the way that 17β-estradiol (E2) exerts its role in the nerve cell homeostasis.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
T1  - Modulation of Ca2+ ion flux through mitochondrial membrane of the rat brain steam synaptosomes by 17β-estradiol
VL  - 1
SP  - 352
EP  - 354
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9368
ER  - 

@conference{
author = "Petrović, S. and Milošević, Maja and Horvat, Anica",
year = "2008",
abstract = "In the present study the modulation of Ca2+ ion flux in the synaptosomal mitochondria isolated from the ovariectyomized rat Brain Steam and the possible roll of membrane bound estradiol was examined. Physiological concentrations of 17β-estradiol binds specifically to isolated mitochondria (Vmax 3.37± 0.25 pmol/mg protein, Km 1.85± 0.06 nmol/l of free estradiol). Addition of 17β-estradiol (10 pmol/l - 1 nmol/ l) in vitro decreased mitochondrial calcium ion efflux significantly (25%) after 10 minutes. Modulation of calcium ion efflux and mitochondrial ion retention may be the way that 17β-estradiol (E2) exerts its role in the nerve cell homeostasis.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry",
title = "Modulation of Ca2+ ion flux through mitochondrial membrane of the rat brain steam synaptosomes by 17β-estradiol",
volume = "1",
pages = "352-354",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9368"
}

Petrović, S., Milošević, M.,& Horvat, A.. (2008). Modulation of Ca2+ ion flux through mitochondrial membrane of the rat brain steam synaptosomes by 17β-estradiol. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 352-354.
https://hdl.handle.net/21.15107/rcub_vinar_9368

Petrović S, Milošević M, Horvat A. Modulation of Ca2+ ion flux through mitochondrial membrane of the rat brain steam synaptosomes by 17β-estradiol. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry. 2008;1:352-354.
https://hdl.handle.net/21.15107/rcub_vinar_9368 .

Petrović, S., Milošević, Maja, Horvat, Anica, "Modulation of Ca2+ ion flux through mitochondrial membrane of the rat brain steam synaptosomes by 17β-estradiol" in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry, 1 (2008):352-354,
https://hdl.handle.net/21.15107/rcub_vinar_9368 .

TY  - CONF
AU  - Milošević, Maja
AU  - Petrović, S.
AU  - Horvat, Anica
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9379
AB  - The potential protective effects of ethylenediamine tetraacetic acid (EDTA) on the CuSO4 cell toxicity was examined on rat myometrial plasma membranes. Activity of plasma membrane ecto-ATPase, as modulator of purinergic signaling in the presence of increasing concentrations of copper salt and in the presence or absence of EDTA was studied. Our results show that 1mmol/l EDTA may exert protective effects on Cu2+-induced toxicity by increasing half-maximum inhibitory activities of this ion.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
T1  - Protection of copper ions induced inhibition of rat myometrial ecto-atpase activity by edta
VL  - 1
SP  - 444
EP  - 446
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9379
ER  - 

@conference{
author = "Milošević, Maja and Petrović, S. and Horvat, Anica",
year = "2008",
abstract = "The potential protective effects of ethylenediamine tetraacetic acid (EDTA) on the CuSO4 cell toxicity was examined on rat myometrial plasma membranes. Activity of plasma membrane ecto-ATPase, as modulator of purinergic signaling in the presence of increasing concentrations of copper salt and in the presence or absence of EDTA was studied. Our results show that 1mmol/l EDTA may exert protective effects on Cu2+-induced toxicity by increasing half-maximum inhibitory activities of this ion.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry",
title = "Protection of copper ions induced inhibition of rat myometrial ecto-atpase activity by edta",
volume = "1",
pages = "444-446",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9379"
}

Milošević, M., Petrović, S.,& Horvat, A.. (2008). Protection of copper ions induced inhibition of rat myometrial ecto-atpase activity by edta. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 444-446.
https://hdl.handle.net/21.15107/rcub_vinar_9379

Milošević M, Petrović S, Horvat A. Protection of copper ions induced inhibition of rat myometrial ecto-atpase activity by edta. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry. 2008;1:444-446.
https://hdl.handle.net/21.15107/rcub_vinar_9379 .

Milošević, Maja, Petrović, S., Horvat, Anica, "Protection of copper ions induced inhibition of rat myometrial ecto-atpase activity by edta" in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry, 1 (2008):444-446,
https://hdl.handle.net/21.15107/rcub_vinar_9379 .

TY  - CONF
AU  - Veličković, Nataša
AU  - Petrović, S.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9374
AB  - Ionizing radiation (IR) is commonly used in the treatment of brain tumors but it can cause significant damage to surrounding normal brain tissue. In this study, heads of young Wistar rats (18 days old) were subjected to a single dose of gamma irradiation (10 Gy) similar to that used in prophylactic brain irradiation of children with acute lymphoblastic leukemia. The kinetics of apoptosis associated proteins (p53 and Bax) were examined by Western blotting and RT-PCR. We observed that p53 mRNA expression was unchanged after irradiation, while induction of p53 protein was rapid, leading to the accumulation of p53 protein in the cytoplasm. In addition, Bax mRNA and protein levels were also increased following cranial irradiation. These results indicate that cranial irradiation, used in terms of prophylactic therapy, is associated with activation of the p53 system, alongside with induction of positive apoptosis regulator Bax.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
T1  - Radiation-mediated induction of p53 pathway in the rat hippocampus
VL  - 1
SP  - 411
EP  - 413
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9374
ER  - 

@conference{
author = "Veličković, Nataša and Petrović, S.",
year = "2008",
abstract = "Ionizing radiation (IR) is commonly used in the treatment of brain tumors but it can cause significant damage to surrounding normal brain tissue. In this study, heads of young Wistar rats (18 days old) were subjected to a single dose of gamma irradiation (10 Gy) similar to that used in prophylactic brain irradiation of children with acute lymphoblastic leukemia. The kinetics of apoptosis associated proteins (p53 and Bax) were examined by Western blotting and RT-PCR. We observed that p53 mRNA expression was unchanged after irradiation, while induction of p53 protein was rapid, leading to the accumulation of p53 protein in the cytoplasm. In addition, Bax mRNA and protein levels were also increased following cranial irradiation. These results indicate that cranial irradiation, used in terms of prophylactic therapy, is associated with activation of the p53 system, alongside with induction of positive apoptosis regulator Bax.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry",
title = "Radiation-mediated induction of p53 pathway in the rat hippocampus",
volume = "1",
pages = "411-413",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9374"
}

Veličković, N.,& Petrović, S.. (2008). Radiation-mediated induction of p53 pathway in the rat hippocampus. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 411-413.
https://hdl.handle.net/21.15107/rcub_vinar_9374

Veličković N, Petrović S. Radiation-mediated induction of p53 pathway in the rat hippocampus. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry. 2008;1:411-413.
https://hdl.handle.net/21.15107/rcub_vinar_9374 .

Veličković, Nataša, Petrović, S., "Radiation-mediated induction of p53 pathway in the rat hippocampus" in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry, 1 (2008):411-413,
https://hdl.handle.net/21.15107/rcub_vinar_9374 .

TY  - CONF
AU  - Milošević, Maja
AU  - Petrović, S.
AU  - Horvat, Anica
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9497
AB  - Effects of increasing concentrations of metal salts SrCl2, CsCl, CrCl3, CdCl2, and HgCl2 on rat ovarian plasma membrane ecto-ATPase activity were investigated. CrCl3, CdCl2, and HgCl2 exert total inhibition of enzyme activity in the presence of 0.01 M and 0.1 M respectively. SrCl2 and CsCl exhibit up to 25% of inhibition. According to the IC50, ecto-ATPase possesses greater sensibility to Cd2+ (IC50 is 0.887 mM) > Cr3+ (IC50 is 1.936 mM) > Hg2+ (IC50 is 4.39 mM). All investigated ions exert negative cooperativity (n<1). Physico-chemical properties of the metal are of importance in metal toxicity. Cr3+ and Cd2+ ions, with lower radius may inhibit ecto-ATPase activity by binding to hydrolytic site or by replacing Mg2+ in Mg-ATP, a substrate of the enzyme. Hg2+, as a larger ion probably inhibits the enzyme activity through conformational changing the enzyme by binding to S-S or -SH groups on the site distinct to hydrolytic one. By inhibiting the enzyme activity these metals may affect maturation and release of oocytes as well as synthesis and release of gonadal hormones and decrease the fertility of mammals
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Effects of metal ions on ecto-ATPase activity in plasma membrane isolated from the rat ovary
VL  - 1
SP  - 368
EP  - 370
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9497
ER  - 

@conference{
author = "Milošević, Maja and Petrović, S. and Horvat, Anica",
year = "2004",
abstract = "Effects of increasing concentrations of metal salts SrCl2, CsCl, CrCl3, CdCl2, and HgCl2 on rat ovarian plasma membrane ecto-ATPase activity were investigated. CrCl3, CdCl2, and HgCl2 exert total inhibition of enzyme activity in the presence of 0.01 M and 0.1 M respectively. SrCl2 and CsCl exhibit up to 25% of inhibition. According to the IC50, ecto-ATPase possesses greater sensibility to Cd2+ (IC50 is 0.887 mM) > Cr3+ (IC50 is 1.936 mM) > Hg2+ (IC50 is 4.39 mM). All investigated ions exert negative cooperativity (n<1). Physico-chemical properties of the metal are of importance in metal toxicity. Cr3+ and Cd2+ ions, with lower radius may inhibit ecto-ATPase activity by binding to hydrolytic site or by replacing Mg2+ in Mg-ATP, a substrate of the enzyme. Hg2+, as a larger ion probably inhibits the enzyme activity through conformational changing the enzyme by binding to S-S or -SH groups on the site distinct to hydrolytic one. By inhibiting the enzyme activity these metals may affect maturation and release of oocytes as well as synthesis and release of gonadal hormones and decrease the fertility of mammals",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Effects of metal ions on ecto-ATPase activity in plasma membrane isolated from the rat ovary",
volume = "1",
pages = "368-370",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9497"
}

Milošević, M., Petrović, S.,& Horvat, A.. (2004). Effects of metal ions on ecto-ATPase activity in plasma membrane isolated from the rat ovary. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 368-370.
https://hdl.handle.net/21.15107/rcub_vinar_9497

Milošević M, Petrović S, Horvat A. Effects of metal ions on ecto-ATPase activity in plasma membrane isolated from the rat ovary. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:368-370.
https://hdl.handle.net/21.15107/rcub_vinar_9497 .

Milošević, Maja, Petrović, S., Horvat, Anica, "Effects of metal ions on ecto-ATPase activity in plasma membrane isolated from the rat ovary" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):368-370,
https://hdl.handle.net/21.15107/rcub_vinar_9497 .

TY  - CONF
AU  - Horvat, Anica
AU  - Milošević, Maja
AU  - Petrović, Snježana
AU  - Demajo, Miroslav
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9499
AB  - Ions of zinc and cooper represents micro elements present in all organisms but they can be accumulated in various tissues from the environment by food or pollution. In this work we examined the effects of chloride salts of Cu2+ and Zn2+ on the activity of ecto-ATPase, integral plasma membrane protein, in brain and ovarian cells. Both ions exhibit similar effects on brain or ovarian enzyme activity. Cooper in brain and ovary totally inhibits enzyme activity at the concentration of 1 mM and 0.1 M respectively. IC50 for brain is 36 µM while in ovary it is 192 µM. Zinc inhibits in both tissues 50% of the control enzyme activity at concentrations of 1 mM in brain and 0.1 M in ovary with IC50 of 1mM and 14 mM respectively. These metals possess an affinity for -SH groups in the enzyme protein, may replace Mg2+ in enzyme substrate, MgATP or to bind for enzyme substrate site. According to the total inhibition of ecto-ATPase activity by Cu 2+, it may be proposed that its site of action is on the substrate or substrate binding site, while Zn2+ modulates enzyme activity by acting on the –SH or S-S groups of the enzyme. Inhibiting or decreasing ecto-ATPase activity in brain and ovary, these two metal ions may be toxic and seriously disturb the proper functioning of the investigated tissues.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+
VL  - 1
SP  - 374
EP  - 376
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9499
ER  - 

@conference{
author = "Horvat, Anica and Milošević, Maja and Petrović, Snježana and Demajo, Miroslav",
year = "2004",
abstract = "Ions of zinc and cooper represents micro elements present in all organisms but they can be accumulated in various tissues from the environment by food or pollution. In this work we examined the effects of chloride salts of Cu2+ and Zn2+ on the activity of ecto-ATPase, integral plasma membrane protein, in brain and ovarian cells. Both ions exhibit similar effects on brain or ovarian enzyme activity. Cooper in brain and ovary totally inhibits enzyme activity at the concentration of 1 mM and 0.1 M respectively. IC50 for brain is 36 µM while in ovary it is 192 µM. Zinc inhibits in both tissues 50% of the control enzyme activity at concentrations of 1 mM in brain and 0.1 M in ovary with IC50 of 1mM and 14 mM respectively. These metals possess an affinity for -SH groups in the enzyme protein, may replace Mg2+ in enzyme substrate, MgATP or to bind for enzyme substrate site. According to the total inhibition of ecto-ATPase activity by Cu 2+, it may be proposed that its site of action is on the substrate or substrate binding site, while Zn2+ modulates enzyme activity by acting on the –SH or S-S groups of the enzyme. Inhibiting or decreasing ecto-ATPase activity in brain and ovary, these two metal ions may be toxic and seriously disturb the proper functioning of the investigated tissues.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+",
volume = "1",
pages = "374-376",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9499"
}

Horvat, A., Milošević, M., Petrović, S.,& Demajo, M.. (2004). Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 374-376.
https://hdl.handle.net/21.15107/rcub_vinar_9499

Horvat A, Milošević M, Petrović S, Demajo M. Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:374-376.
https://hdl.handle.net/21.15107/rcub_vinar_9499 .

Horvat, Anica, Milošević, Maja, Petrović, Snježana, Demajo, Miroslav, "Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):374-376,
https://hdl.handle.net/21.15107/rcub_vinar_9499 .