TY  - JOUR
AU  - Jokanović, Vukoman R.
AU  - Čolović, Božana M.
AU  - Marković, Dejan
AU  - Petrović, Milan
AU  - Jokanović, Milan
AU  - Milosavljević, Petar
AU  - Sopta, Jelena
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1040
AB  - A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss(R) from the aspect of some parameters of immunological response.
T2  - Journal of Nanomaterials
T1  - In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA
DO  - 10.1155/2016/3948768
ER  - 

@article{
author = "Jokanović, Vukoman R. and Čolović, Božana M. and Marković, Dejan and Petrović, Milan and Jokanović, Milan and Milosavljević, Petar and Sopta, Jelena",
year = "2016",
abstract = "A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss(R) from the aspect of some parameters of immunological response.",
journal = "Journal of Nanomaterials",
title = "In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA",
doi = "10.1155/2016/3948768"
}

Jokanović, V. R., Čolović, B. M., Marković, D., Petrović, M., Jokanović, M., Milosavljević, P.,& Sopta, J.. (2016). In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA. in Journal of Nanomaterials.
https://doi.org/10.1155/2016/3948768

Jokanović VR, Čolović BM, Marković D, Petrović M, Jokanović M, Milosavljević P, Sopta J. In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA. in Journal of Nanomaterials. 2016;.
doi:10.1155/2016/3948768 .

Jokanović, Vukoman R., Čolović, Božana M., Marković, Dejan, Petrović, Milan, Jokanović, Milan, Milosavljević, Petar, Sopta, Jelena, "In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA" in Journal of Nanomaterials (2016),
https://doi.org/10.1155/2016/3948768 . .

TY  - JOUR
AU  - Vranić-Mandušić, Vesna
AU  - Subota, Vesna
AU  - Savovski, K
AU  - Medic, L
AU  - Dramićanin, Tatjana
AU  - Jozanov-Stankov, Olga
AU  - Popov-Celeketic, D
AU  - Jokanović, Milan
AU  - Dimitrijević, Bogomir B.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2701
AB  - Tiazofurin (TZF) is a cytostatic drug that leads to depletion of the GTP pool in tumor and normal cells via its active metabolite tiazofurin-adenine dinucleotide (TAD). TAD was detected in different cell lines, but not in erythrocytes, so the mechanism of erythrocytotoxicity of TZF remains unclear. The purpose of this study was to evaluate in vitro and in vivo action of tiazofurin on rat erythrocytes (RBC). After two decades of clinical trials the question of erythrocytotoxicity of TZF had remained unexplained making this study justified. Since we have previously demonstrated early erythrocytotoxic effects in male Wistar rats, we extend this finding on isolated RBC. Isolated erythrocytes from untreated animals were treated in buffered solution or plasma containing TZF In addition, groups of 10 rats were treated with 200 and 1000 mg/kg of TZF and hematologic parameters were analyzed by flowcytometry and by the analysis of the peripheral blood smears. Early signs of hemolysis or aberrant structures were monitored by scanning probe microscopy (SPM). We suggest that correlation exists between early erythrocytotoxicity and irregularities in erythrocyte morphology and membrane integrity. We also found that TZF affects responsiveness to oxidative stress. This is in concordance with flowcytometric findings describing anisocytosis and anisochromosis of RBC. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
T2  - Toxicology Letters
T1  - Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy
VL  - 146
IS  - 3
SP  - 275
EP  - 284
DO  - 10.1016/j.toxlet.2003.10.013
ER  - 

@article{
author = "Vranić-Mandušić, Vesna and Subota, Vesna and Savovski, K and Medic, L and Dramićanin, Tatjana and Jozanov-Stankov, Olga and Popov-Celeketic, D and Jokanović, Milan and Dimitrijević, Bogomir B.",
year = "2004",
abstract = "Tiazofurin (TZF) is a cytostatic drug that leads to depletion of the GTP pool in tumor and normal cells via its active metabolite tiazofurin-adenine dinucleotide (TAD). TAD was detected in different cell lines, but not in erythrocytes, so the mechanism of erythrocytotoxicity of TZF remains unclear. The purpose of this study was to evaluate in vitro and in vivo action of tiazofurin on rat erythrocytes (RBC). After two decades of clinical trials the question of erythrocytotoxicity of TZF had remained unexplained making this study justified. Since we have previously demonstrated early erythrocytotoxic effects in male Wistar rats, we extend this finding on isolated RBC. Isolated erythrocytes from untreated animals were treated in buffered solution or plasma containing TZF In addition, groups of 10 rats were treated with 200 and 1000 mg/kg of TZF and hematologic parameters were analyzed by flowcytometry and by the analysis of the peripheral blood smears. Early signs of hemolysis or aberrant structures were monitored by scanning probe microscopy (SPM). We suggest that correlation exists between early erythrocytotoxicity and irregularities in erythrocyte morphology and membrane integrity. We also found that TZF affects responsiveness to oxidative stress. This is in concordance with flowcytometric findings describing anisocytosis and anisochromosis of RBC. (C) 2003 Elsevier Ireland Ltd. All rights reserved.",
journal = "Toxicology Letters",
title = "Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy",
volume = "146",
number = "3",
pages = "275-284",
doi = "10.1016/j.toxlet.2003.10.013"
}

Vranić-Mandušić, V., Subota, V., Savovski, K., Medic, L., Dramićanin, T., Jozanov-Stankov, O., Popov-Celeketic, D., Jokanović, M.,& Dimitrijević, B. B.. (2004). Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy. in Toxicology Letters, 146(3), 275-284.
https://doi.org/10.1016/j.toxlet.2003.10.013

Vranić-Mandušić V, Subota V, Savovski K, Medic L, Dramićanin T, Jozanov-Stankov O, Popov-Celeketic D, Jokanović M, Dimitrijević BB. Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy. in Toxicology Letters. 2004;146(3):275-284.
doi:10.1016/j.toxlet.2003.10.013 .

Vranić-Mandušić, Vesna, Subota, Vesna, Savovski, K, Medic, L, Dramićanin, Tatjana, Jozanov-Stankov, Olga, Popov-Celeketic, D, Jokanović, Milan, Dimitrijević, Bogomir B., "Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy" in Toxicology Letters, 146, no. 3 (2004):275-284,
https://doi.org/10.1016/j.toxlet.2003.10.013 . .

TY  - JOUR
AU  - Milovanović, GA
AU  - Čakar, Mira M.
AU  - Vučić, Nevenka B.
AU  - Jokanović, Milan
PY  - 2000
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2397
AB  - In this work it was established that, in the presence of ammonium carbonate, traces of manganese(II) catalyse the oxidation of Nile Blue A by hydrogen peroxide, which enables its: kinetic determination in the concentration range from 6.6 to 65.9 ng cm(-3), the detection limit being 8.0 x 10(-2) ng cm(-3). Antiviral/antitumour substances modify the catalytic activity of manganese (TT): 1-beta -D-ribofuranosyl-1,2,4-triazole-3-carboxamide, ribavirin, increases the catalytic effect of manganese(II), while 2-beta -D-ribofuranosyl-thiazole-4-carboxamide, tiazofurin, acts as an inhibitor. On the basis of these effects, a kinetic method for determining ribavirin concentrations from 0.5 x 10(-1) to 4.0 x 10(-1) mug cm(-3) and tiazofurin concentrations from 0.3 to 2.6 mug cm(-3) is proposed. The kinetics of the indicator reaction were studied ill the presence of the substances examined, the kinetic equations established, and the constants of the corresponding reaction rates calculated. The effect of temperature on these reactions was also investigated. The method was applied to the determination of manganese(II) in mineral water and ribavirin in pharmaceutical preparations.
T2  - Microchimica Acta
T1  - Selective indicator reaction for kinetic determination of traces of manganese(II), ribavirin and tiazofurin
VL  - 135
IS  - 3-4
SP  - 173
EP  - 178
DO  - 10.1007/s006040070007
ER  - 

@article{
author = "Milovanović, GA and Čakar, Mira M. and Vučić, Nevenka B. and Jokanović, Milan",
year = "2000",
abstract = "In this work it was established that, in the presence of ammonium carbonate, traces of manganese(II) catalyse the oxidation of Nile Blue A by hydrogen peroxide, which enables its: kinetic determination in the concentration range from 6.6 to 65.9 ng cm(-3), the detection limit being 8.0 x 10(-2) ng cm(-3). Antiviral/antitumour substances modify the catalytic activity of manganese (TT): 1-beta -D-ribofuranosyl-1,2,4-triazole-3-carboxamide, ribavirin, increases the catalytic effect of manganese(II), while 2-beta -D-ribofuranosyl-thiazole-4-carboxamide, tiazofurin, acts as an inhibitor. On the basis of these effects, a kinetic method for determining ribavirin concentrations from 0.5 x 10(-1) to 4.0 x 10(-1) mug cm(-3) and tiazofurin concentrations from 0.3 to 2.6 mug cm(-3) is proposed. The kinetics of the indicator reaction were studied ill the presence of the substances examined, the kinetic equations established, and the constants of the corresponding reaction rates calculated. The effect of temperature on these reactions was also investigated. The method was applied to the determination of manganese(II) in mineral water and ribavirin in pharmaceutical preparations.",
journal = "Microchimica Acta",
title = "Selective indicator reaction for kinetic determination of traces of manganese(II), ribavirin and tiazofurin",
volume = "135",
number = "3-4",
pages = "173-178",
doi = "10.1007/s006040070007"
}

Milovanović, G., Čakar, M. M., Vučić, N. B.,& Jokanović, M.. (2000). Selective indicator reaction for kinetic determination of traces of manganese(II), ribavirin and tiazofurin. in Microchimica Acta, 135(3-4), 173-178.
https://doi.org/10.1007/s006040070007

Milovanović G, Čakar MM, Vučić NB, Jokanović M. Selective indicator reaction for kinetic determination of traces of manganese(II), ribavirin and tiazofurin. in Microchimica Acta. 2000;135(3-4):173-178.
doi:10.1007/s006040070007 .

Milovanović, GA, Čakar, Mira M., Vučić, Nevenka B., Jokanović, Milan, "Selective indicator reaction for kinetic determination of traces of manganese(II), ribavirin and tiazofurin" in Microchimica Acta, 135, no. 3-4 (2000):173-178,
https://doi.org/10.1007/s006040070007 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Stankovic, M
AU  - Vasić, Vesna M.
AU  - Čakar, Mira M.
AU  - Jokanović, Milan
PY  - 2000
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2383
AB  - Cytotoxic effects of the antiviremic drug ribavirin (1-beta -D-ribofuranosyl-1,2,4-triazole-3-carboxamide) were evaluated in vitro measuring micronucleus formation and cell proliferation kinetics in whole blood cultures employing cytokinesis block (CB) micronucleus test. The cells were exposed to ribavirin doses ranging from 0.05-0.65 mu mol/ml at three different incubation times. The frequency of micronuclei in treated samples demonstrated relatively low ability of ribavirin to induce micronuclei. However, the lowest concentration of ribavirin markedly changed the frequency of mononucleated and multinucleated cells, particularly binucleated ones, which declined significantly. The decline in the frequency of binucleate cells was followed with accumulation of greater number of mononucleate cells. Decreased proliferation potential of lymphocytes treated with ribavirin indicates that cells are arrested prior to metaphase. The present investigation showed that ribavirin is capable to induce a delay in cellular proliferation at all the doses assayed. The study demonstrated that CB micronucleus assay is simple and rapid method that can be used to assess toxic effect of drugs in vitro.
T2  - Neoplasma
T1  - Influence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytes
VL  - 47
IS  - 5
SP  - 283
EP  - 287
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2383
ER  - 

@article{
author = "Joksić, Gordana and Stankovic, M and Vasić, Vesna M. and Čakar, Mira M. and Jokanović, Milan",
year = "2000",
abstract = "Cytotoxic effects of the antiviremic drug ribavirin (1-beta -D-ribofuranosyl-1,2,4-triazole-3-carboxamide) were evaluated in vitro measuring micronucleus formation and cell proliferation kinetics in whole blood cultures employing cytokinesis block (CB) micronucleus test. The cells were exposed to ribavirin doses ranging from 0.05-0.65 mu mol/ml at three different incubation times. The frequency of micronuclei in treated samples demonstrated relatively low ability of ribavirin to induce micronuclei. However, the lowest concentration of ribavirin markedly changed the frequency of mononucleated and multinucleated cells, particularly binucleated ones, which declined significantly. The decline in the frequency of binucleate cells was followed with accumulation of greater number of mononucleate cells. Decreased proliferation potential of lymphocytes treated with ribavirin indicates that cells are arrested prior to metaphase. The present investigation showed that ribavirin is capable to induce a delay in cellular proliferation at all the doses assayed. The study demonstrated that CB micronucleus assay is simple and rapid method that can be used to assess toxic effect of drugs in vitro.",
journal = "Neoplasma",
title = "Influence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytes",
volume = "47",
number = "5",
pages = "283-287",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2383"
}

Joksić, G., Stankovic, M., Vasić, V. M., Čakar, M. M.,& Jokanović, M.. (2000). Influence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytes. in Neoplasma, 47(5), 283-287.
https://hdl.handle.net/21.15107/rcub_vinar_2383

Joksić G, Stankovic M, Vasić VM, Čakar MM, Jokanović M. Influence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytes. in Neoplasma. 2000;47(5):283-287.
https://hdl.handle.net/21.15107/rcub_vinar_2383 .

Joksić, Gordana, Stankovic, M, Vasić, Vesna M., Čakar, Mira M., Jokanović, Milan, "Influence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytes" in Neoplasma, 47, no. 5 (2000):283-287,
https://hdl.handle.net/21.15107/rcub_vinar_2383 .