TY  - JOUR
AU  - Radić, Jelena
AU  - Kožik, Bojana
AU  - Nikolić, Ivan
AU  - Kolarov-Bjelobrk, Ivana
AU  - Vasiljević, Tijana
AU  - Vranjković, Bojana
AU  - Despotović, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11377
AB  - LOXL2, a copper-dependent amine oxidase, has emerged as a promising therapeutic
target in hepatocellular carcinoma (HCC). Increased LOXL2 expression in HCC has been linked
with an aggressive phenotype and represents a poor prognostic factor. Here, we focus on the
mechanisms through which LOXL2 orchestrates multiple oncogenic functions in HCC development.
We performed a review of the current knowledge on the roles LOXL2 performs in the modulation of
the HCC tumor microenvironment, formation of premetastatic niches, and epithelial–mesenchymal
transition. We also highlighted the complex interplay between LOXL2 and hypoxia, angiogenesis,
and vasculogenic mimicry in HCC. At the end of the review, we summarize the current LOXL2
inhibitors and discuss their potential in HCC precision treatment.
T2  - International Journal of Molecular Sciences
T1  - Multiple Roles of LOXL2 in the Progression of Hepatocellular Carcinoma and Its Potential for Therapeutic Targeting
VL  - 24
IS  - 14
SP  - 11745
DO  - 10.3390/ijms241411745
ER  - 

@article{
author = "Radić, Jelena and Kožik, Bojana and Nikolić, Ivan and Kolarov-Bjelobrk, Ivana and Vasiljević, Tijana and Vranjković, Bojana and Despotović, Sanja",
year = "2023",
abstract = "LOXL2, a copper-dependent amine oxidase, has emerged as a promising therapeutic
target in hepatocellular carcinoma (HCC). Increased LOXL2 expression in HCC has been linked
with an aggressive phenotype and represents a poor prognostic factor. Here, we focus on the
mechanisms through which LOXL2 orchestrates multiple oncogenic functions in HCC development.
We performed a review of the current knowledge on the roles LOXL2 performs in the modulation of
the HCC tumor microenvironment, formation of premetastatic niches, and epithelial–mesenchymal
transition. We also highlighted the complex interplay between LOXL2 and hypoxia, angiogenesis,
and vasculogenic mimicry in HCC. At the end of the review, we summarize the current LOXL2
inhibitors and discuss their potential in HCC precision treatment.",
journal = "International Journal of Molecular Sciences",
title = "Multiple Roles of LOXL2 in the Progression of Hepatocellular Carcinoma and Its Potential for Therapeutic Targeting",
volume = "24",
number = "14",
pages = "11745",
doi = "10.3390/ijms241411745"
}

Radić, J., Kožik, B., Nikolić, I., Kolarov-Bjelobrk, I., Vasiljević, T., Vranjković, B.,& Despotović, S.. (2023). Multiple Roles of LOXL2 in the Progression of Hepatocellular Carcinoma and Its Potential for Therapeutic Targeting. in International Journal of Molecular Sciences, 24(14), 11745.
https://doi.org/10.3390/ijms241411745

Radić J, Kožik B, Nikolić I, Kolarov-Bjelobrk I, Vasiljević T, Vranjković B, Despotović S. Multiple Roles of LOXL2 in the Progression of Hepatocellular Carcinoma and Its Potential for Therapeutic Targeting. in International Journal of Molecular Sciences. 2023;24(14):11745.
doi:10.3390/ijms241411745 .

Radić, Jelena, Kožik, Bojana, Nikolić, Ivan, Kolarov-Bjelobrk, Ivana, Vasiljević, Tijana, Vranjković, Bojana, Despotović, Sanja, "Multiple Roles of LOXL2 in the Progression of Hepatocellular Carcinoma and Its Potential for Therapeutic Targeting" in International Journal of Molecular Sciences, 24, no. 14 (2023):11745,
https://doi.org/10.3390/ijms241411745 . .

TY  - CONF
AU  - Kožik, Bojana
AU  - Kokanov, Nikola
AU  - Knežević-Ušaj, Slavica
AU  - Nikolić, Ivan
AU  - Davidović, Radoslav
AU  - Jovanović Ćupić, Snežana
AU  - Krajnović, Milena
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12805
AB  - Background: Preoperative chemoradiotherapy (CRT) represents the standard treatment for patients with locally advanced rectal cancer. Since only subset of patients has benefit from this preoperative treatment, development of reliable molecular biomarkers is required. In this retrospective study, we investigated methylation status of p16 and p14 tumor suppressor genes in locally advanced rectal cancer, in order to evaluate their potential predictive and prognostic role. Methods: Methylation-specific PCR was used to examine methylation status of p16 and p14 genes in pretherapeutic and preoperative biopsy specimens of 60 patients with locally advanced rectal cancer. Results: Aberrant methylation of p16 and p14 genes was detected in 43.3% (26/60) and 39.6% (23/58) of cases, respectively. In general, p16 and p14 methylation status did not affect the response to CRT, recurrences rate and overall survival. However, patients with simultaneous presence of either p16 or p14 methylation and high vascular endothelial growth factor (VEGF) expression showed significantly worse response to CRT (p = 0.005 and p = 0.038, respectively). In addition, tendency toward more frequent local recurrences and metastasis was observed in cases with concurrent presence of methylation of either p16 or p14 gene and high VEGF expression (p = 0.075 and p = 0.072, respectively), while patients with both of p16 methylation and high VEGF expression had significantly shorter overall survival (p = 0.010). Conclusion: Obtained results strongly suggest the importance of p16 and p14 methylation analyses in combination with other parameters, particularly VEGF expression, in order to better predict treatment response and patient outcome.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Combined analysis of p16 and p14 methylation and VEGF expression status could predict more aggressive phenotype of locally advanced rectal cancers
SP  - 100
EP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12805
ER  - 

@conference{
author = "Kožik, Bojana and Kokanov, Nikola and Knežević-Ušaj, Slavica and Nikolić, Ivan and Davidović, Radoslav and Jovanović Ćupić, Snežana and Krajnović, Milena",
year = "2017",
abstract = "Background: Preoperative chemoradiotherapy (CRT) represents the standard treatment for patients with locally advanced rectal cancer. Since only subset of patients has benefit from this preoperative treatment, development of reliable molecular biomarkers is required. In this retrospective study, we investigated methylation status of p16 and p14 tumor suppressor genes in locally advanced rectal cancer, in order to evaluate their potential predictive and prognostic role. Methods: Methylation-specific PCR was used to examine methylation status of p16 and p14 genes in pretherapeutic and preoperative biopsy specimens of 60 patients with locally advanced rectal cancer. Results: Aberrant methylation of p16 and p14 genes was detected in 43.3% (26/60) and 39.6% (23/58) of cases, respectively. In general, p16 and p14 methylation status did not affect the response to CRT, recurrences rate and overall survival. However, patients with simultaneous presence of either p16 or p14 methylation and high vascular endothelial growth factor (VEGF) expression showed significantly worse response to CRT (p = 0.005 and p = 0.038, respectively). In addition, tendency toward more frequent local recurrences and metastasis was observed in cases with concurrent presence of methylation of either p16 or p14 gene and high VEGF expression (p = 0.075 and p = 0.072, respectively), while patients with both of p16 methylation and high VEGF expression had significantly shorter overall survival (p = 0.010). Conclusion: Obtained results strongly suggest the importance of p16 and p14 methylation analyses in combination with other parameters, particularly VEGF expression, in order to better predict treatment response and patient outcome.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Combined analysis of p16 and p14 methylation and VEGF expression status could predict more aggressive phenotype of locally advanced rectal cancers",
pages = "100-100",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12805"
}

Kožik, B., Kokanov, N., Knežević-Ušaj, S., Nikolić, I., Davidović, R., Jovanović Ćupić, S.,& Krajnović, M.. (2017). Combined analysis of p16 and p14 methylation and VEGF expression status could predict more aggressive phenotype of locally advanced rectal cancers. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 100-100.
https://hdl.handle.net/21.15107/rcub_vinar_12805

Kožik B, Kokanov N, Knežević-Ušaj S, Nikolić I, Davidović R, Jovanović Ćupić S, Krajnović M. Combined analysis of p16 and p14 methylation and VEGF expression status could predict more aggressive phenotype of locally advanced rectal cancers. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:100-100.
https://hdl.handle.net/21.15107/rcub_vinar_12805 .

Kožik, Bojana, Kokanov, Nikola, Knežević-Ušaj, Slavica, Nikolić, Ivan, Davidović, Radoslav, Jovanović Ćupić, Snežana, Krajnović, Milena, "Combined analysis of p16 and p14 methylation and VEGF expression status could predict more aggressive phenotype of locally advanced rectal cancers" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):100-100,
https://hdl.handle.net/21.15107/rcub_vinar_12805 .