TY  - JOUR
AU  - Virijević, Kristina
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Ferizović, Harisa
AU  - Janković, Milica
AU  - Vasiljević, Perica
AU  - Dronjak, Slađana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12934
AB  - Wistar-Kyoto (WKY) rats subjected to chronic mild stress (CMS) represent a valid model of treatment-resistant depression (TRD). Considering that depression is more prevalent in women than in men, in the present study, female rats were used. We investigated the effect of CMS on behavior and different factors involved in neuroinflammatory processes and neuroplasticity in the hippocampus and medial prefrontal cortex (mPFC) of WKY female rats. The results show that unstressed WKY females exhibited hypolocomotion, decreased exploratory behavior, and an increase in the total grooming time. After exposure to CMS, WKY females displayed intensified grooming. To investigate potential neural mechanisms underlying these behavioral changes, we analyzed signaling and inflammatory pathways in the hippocampus and mPFC. The findings indicate reduced BDNF and elevated levels levels of IL-1β in both brain structures and NLRP3 in the mPFC of unstressed WKY female rats. WKY rats subjected to CMS showed a further decrease in BDNF levels and increased IL-1β and NLRP3 in these brain structures. WKY showed reduced pERK1/2 and increased pp38 levels in both brain structures, while CMS revealed a further increase of pp38 in WKY in these brain structures. Expressions of p110β and pAKT were decreased in the hippocampus and mPFC of WKY rats. The CMS further suppressed p110 and the downstream AKT phosphorylation in the hippocampus, but did not affect the p110 and pAKT in the mPFC. Our findings indicate behavioral and molecular differences in genetically vulnerable WKY female rats and in their response to CMS that may be involved in TRD.
T2  - Neuroscience Letters
T1  - Chronic mild stress-induced dysregulation of MAPK and PI3K/AKT signaling in the hippocampus and medial prefrontal cortex of WKY female rats
VL  - 825
SP  - 137709
DO  - 10.1016/j.neulet.2024.137709
ER  - 

@article{
author = "Virijević, Kristina and Spasojević, Nataša and Stefanović, Bojana and Ferizović, Harisa and Janković, Milica and Vasiljević, Perica and Dronjak, Slađana",
year = "2024",
abstract = "Wistar-Kyoto (WKY) rats subjected to chronic mild stress (CMS) represent a valid model of treatment-resistant depression (TRD). Considering that depression is more prevalent in women than in men, in the present study, female rats were used. We investigated the effect of CMS on behavior and different factors involved in neuroinflammatory processes and neuroplasticity in the hippocampus and medial prefrontal cortex (mPFC) of WKY female rats. The results show that unstressed WKY females exhibited hypolocomotion, decreased exploratory behavior, and an increase in the total grooming time. After exposure to CMS, WKY females displayed intensified grooming. To investigate potential neural mechanisms underlying these behavioral changes, we analyzed signaling and inflammatory pathways in the hippocampus and mPFC. The findings indicate reduced BDNF and elevated levels levels of IL-1β in both brain structures and NLRP3 in the mPFC of unstressed WKY female rats. WKY rats subjected to CMS showed a further decrease in BDNF levels and increased IL-1β and NLRP3 in these brain structures. WKY showed reduced pERK1/2 and increased pp38 levels in both brain structures, while CMS revealed a further increase of pp38 in WKY in these brain structures. Expressions of p110β and pAKT were decreased in the hippocampus and mPFC of WKY rats. The CMS further suppressed p110 and the downstream AKT phosphorylation in the hippocampus, but did not affect the p110 and pAKT in the mPFC. Our findings indicate behavioral and molecular differences in genetically vulnerable WKY female rats and in their response to CMS that may be involved in TRD.",
journal = "Neuroscience Letters",
title = "Chronic mild stress-induced dysregulation of MAPK and PI3K/AKT signaling in the hippocampus and medial prefrontal cortex of WKY female rats",
volume = "825",
pages = "137709",
doi = "10.1016/j.neulet.2024.137709"
}

Virijević, K., Spasojević, N., Stefanović, B., Ferizović, H., Janković, M., Vasiljević, P.,& Dronjak, S.. (2024). Chronic mild stress-induced dysregulation of MAPK and PI3K/AKT signaling in the hippocampus and medial prefrontal cortex of WKY female rats. in Neuroscience Letters, 825, 137709.
https://doi.org/10.1016/j.neulet.2024.137709

Virijević K, Spasojević N, Stefanović B, Ferizović H, Janković M, Vasiljević P, Dronjak S. Chronic mild stress-induced dysregulation of MAPK and PI3K/AKT signaling in the hippocampus and medial prefrontal cortex of WKY female rats. in Neuroscience Letters. 2024;825:137709.
doi:10.1016/j.neulet.2024.137709 .

Virijević, Kristina, Spasojević, Nataša, Stefanović, Bojana, Ferizović, Harisa, Janković, Milica, Vasiljević, Perica, Dronjak, Slađana, "Chronic mild stress-induced dysregulation of MAPK and PI3K/AKT signaling in the hippocampus and medial prefrontal cortex of WKY female rats" in Neuroscience Letters, 825 (2024):137709,
https://doi.org/10.1016/j.neulet.2024.137709 . .

TY  - CONF
AU  - Janković, Milica
AU  - Ferizović, Harisa
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Virijević, Kristina
AU  - Dronjak, Slađana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11059
AB  - Modulating the endocannabinoid system is emerging as a promising approach for treating various inflammatory, neurodegenerative, and psychiatric disorders. Major depressive disorder is a prevalent cause of disability worldwide, affecting up to 6% of the population and posing significant health and economic challenges. Since the existing antidepressant treatments are often insufficient, the endocannabinoid system presents an attractive target for potential therapies. To explore this possibility, two-month-old Wistar rats of both sexes were subjected to chronic unpredictable stress (CUS) for six weeks. During the last two weeks of stress protocol rats were injected with either a fatty acid amide hydrolase inhibitor, URB597 (i.p. 0.3 mg/kg), or a vehicle. At the end of the sixth week sucrose intake test was used to assess depressive-like behavior. After sacrificing the animals, Western blot analysis was used to determine the levels of brain-derived neurotrophic factor (BDNF) protein and levels of pro-inflammatory cytokine IL-1β in medial prefrontal cortex. The CUS exposure induced anhedonia in both female and male rats, and URB597 treatment alleviated these symptoms. Moreover, CUS increased the levels of IL1-β in the mPFC of both sexes. Stressed animals that have received URB597 had decreased levels of IL1-β structures compared to the animals receiving only vehicle. CUSexposed male and female rats also had lower levels of BDNF in the mPFC, but URB597 restored BDNF levels in female rats to those observed in control animals. Taken together, these findings suggest that URB597 may have antidepressant effects through reducing neuroinflammation and restoring BDNF levels in mPFC.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Fatty acid amide hydrolase inhibitor URB597 shows antidepressant effects through reduction of neuroinflammation and restoration of BDNF levels in mPFC of chronically stressed rats
SP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11059
ER  - 

@conference{
author = "Janković, Milica and Ferizović, Harisa and Spasojević, Nataša and Stefanović, Bojana and Virijević, Kristina and Dronjak, Slađana",
year = "2023",
abstract = "Modulating the endocannabinoid system is emerging as a promising approach for treating various inflammatory, neurodegenerative, and psychiatric disorders. Major depressive disorder is a prevalent cause of disability worldwide, affecting up to 6% of the population and posing significant health and economic challenges. Since the existing antidepressant treatments are often insufficient, the endocannabinoid system presents an attractive target for potential therapies. To explore this possibility, two-month-old Wistar rats of both sexes were subjected to chronic unpredictable stress (CUS) for six weeks. During the last two weeks of stress protocol rats were injected with either a fatty acid amide hydrolase inhibitor, URB597 (i.p. 0.3 mg/kg), or a vehicle. At the end of the sixth week sucrose intake test was used to assess depressive-like behavior. After sacrificing the animals, Western blot analysis was used to determine the levels of brain-derived neurotrophic factor (BDNF) protein and levels of pro-inflammatory cytokine IL-1β in medial prefrontal cortex. The CUS exposure induced anhedonia in both female and male rats, and URB597 treatment alleviated these symptoms. Moreover, CUS increased the levels of IL1-β in the mPFC of both sexes. Stressed animals that have received URB597 had decreased levels of IL1-β structures compared to the animals receiving only vehicle. CUSexposed male and female rats also had lower levels of BDNF in the mPFC, but URB597 restored BDNF levels in female rats to those observed in control animals. Taken together, these findings suggest that URB597 may have antidepressant effects through reducing neuroinflammation and restoring BDNF levels in mPFC.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Fatty acid amide hydrolase inhibitor URB597 shows antidepressant effects through reduction of neuroinflammation and restoration of BDNF levels in mPFC of chronically stressed rats",
pages = "118",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11059"
}

Janković, M., Ferizović, H., Spasojević, N., Stefanović, B., Virijević, K.,& Dronjak, S.. (2023). Fatty acid amide hydrolase inhibitor URB597 shows antidepressant effects through reduction of neuroinflammation and restoration of BDNF levels in mPFC of chronically stressed rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 118.
https://hdl.handle.net/21.15107/rcub_vinar_11059

Janković M, Ferizović H, Spasojević N, Stefanović B, Virijević K, Dronjak S. Fatty acid amide hydrolase inhibitor URB597 shows antidepressant effects through reduction of neuroinflammation and restoration of BDNF levels in mPFC of chronically stressed rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:118.
https://hdl.handle.net/21.15107/rcub_vinar_11059 .

Janković, Milica, Ferizović, Harisa, Spasojević, Nataša, Stefanović, Bojana, Virijević, Kristina, Dronjak, Slađana, "Fatty acid amide hydrolase inhibitor URB597 shows antidepressant effects through reduction of neuroinflammation and restoration of BDNF levels in mPFC of chronically stressed rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):118,
https://hdl.handle.net/21.15107/rcub_vinar_11059 .

TY  - CONF
AU  - Virijević, Kristina
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Ferizović, Harisa
AU  - Janković, Milica
AU  - Dronjak, Slađana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11053
AB  - Stress is a major precipitant of depression, the hippocampus (HPC) and prefrontal cortex (PFC) is the main structures affected by depressive disorders. Women are twice more likely to experience depression than men. The WKY rat strain has long been established as a model of depression. Initially bred from the Wistar (WI) rat as the control strain for the spontaneously hypertensive rat, WKY rats demonstrate an exaggerated stress response compared to other strains. WKY strain fails to respond to chronic antidepressant treatment after exposure to chronic mild stress (CMS) and is considered to be nonresponsive to antidepressant drugs. MAPK signaling pathway was most closely related to depression and antidepressant treatment. In the present study, we have examined the effects of CMS on behavior and p38 MAPK signaling in the hippocampus and the medial prefrontal cortex (mPFC) of female WKY rats. We used two very different behavioral tests: forced swim test (FST) and open field test (OFT). WKY unstressed controls exhibited increased immobility duration in the forced swim test and decreased activity in the open-field test compared to unstressed WI rats, while CMS did not further influence behavior. WKY showed increased expression of the p38 only in HPC and further exposure of WKY rats to CMS induces sustained-activation of p38 MAPK in this brain area. The present study demonstrated the brain region-specific protein signatures in the female WKY model with endogenous depression, providing novel insights into the pathogenesis of depression.
PB  - Belgrade : Serbian Neurocardiological Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11053
ER  - 

@conference{
author = "Virijević, Kristina and Spasojević, Nataša and Stefanović, Bojana and Ferizović, Harisa and Janković, Milica and Dronjak, Slađana",
year = "2023",
abstract = "Stress is a major precipitant of depression, the hippocampus (HPC) and prefrontal cortex (PFC) is the main structures affected by depressive disorders. Women are twice more likely to experience depression than men. The WKY rat strain has long been established as a model of depression. Initially bred from the Wistar (WI) rat as the control strain for the spontaneously hypertensive rat, WKY rats demonstrate an exaggerated stress response compared to other strains. WKY strain fails to respond to chronic antidepressant treatment after exposure to chronic mild stress (CMS) and is considered to be nonresponsive to antidepressant drugs. MAPK signaling pathway was most closely related to depression and antidepressant treatment. In the present study, we have examined the effects of CMS on behavior and p38 MAPK signaling in the hippocampus and the medial prefrontal cortex (mPFC) of female WKY rats. We used two very different behavioral tests: forced swim test (FST) and open field test (OFT). WKY unstressed controls exhibited increased immobility duration in the forced swim test and decreased activity in the open-field test compared to unstressed WI rats, while CMS did not further influence behavior. WKY showed increased expression of the p38 only in HPC and further exposure of WKY rats to CMS induces sustained-activation of p38 MAPK in this brain area. The present study demonstrated the brain region-specific protein signatures in the female WKY model with endogenous depression, providing novel insights into the pathogenesis of depression.",
publisher = "Belgrade : Serbian Neurocardiological Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11053"
}

Virijević, K., Spasojević, N., Stefanović, B., Ferizović, H., Janković, M.,& Dronjak, S.. (2023). Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neurocardiological Society., 84.
https://hdl.handle.net/21.15107/rcub_vinar_11053

Virijević K, Spasojević N, Stefanović B, Ferizović H, Janković M, Dronjak S. Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:84.
https://hdl.handle.net/21.15107/rcub_vinar_11053 .

Virijević, Kristina, Spasojević, Nataša, Stefanović, Bojana, Ferizović, Harisa, Janković, Milica, Dronjak, Slađana, "Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):84,
https://hdl.handle.net/21.15107/rcub_vinar_11053 .

TY  - CONF
AU  - Virijević, Kristina
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Ferizović, Harisa
AU  - Janković, Milica
AU  - Dronjak, Slađana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12630
AB  - Background: The neurobiology underlying depression has not yet been fully identified, but is thought to result from molecular and cellular abnormalities that interact with genetic and environmental factors. Depression is twice as prevalent in women as in men, however, females remain underrepresented in preclinical research. In addition to the neurotransmission theory of depression, the inflammatory processes and the disrupted signaling pathways also play a crucial role in the pathophysiology of depression. The WKY rat strain has long been established as a model of depression. These rats demonstrate an exaggerated response to stress compared to other strains. WKY strain fail to respond to chronic antidepressant treatment after exposure to chronic mild stress (CMS) and considered to be nonresponsive to antidepressant drugs. The hippocampus and the medial prefrontal cortex (mPFC) are thought to be an important regions for depression. Brain-derived neurotrophic factor (BDNF) play a vital role in the pathophysiology of depression. BDNF-stimulated signaling cascades, including the phosphatidylinositol 3-kinase (PI3K)–/serine threonine kinase (Akt), also implicated in depression and treatment respons. In the present study, we have examined the effects of CMS on behavior and BDNF and PI3K/Akt signaling in the hippocampus and mPFC of female WKY rats.

Method: In the experiment, we used three months old Wistar (WI) and WKY female rats. Animals were divided in two groups: control and animals exposed to CMS for 6 weeks. On the last day of stress procedure, animals were tested in elevated plus maze to determine the levels of anxiety. Animals were then sacrificed and hippocampus and mPFC were isolated. Levels of BDNF and pAkt were determined by Western blot method. Data were analyzed using the two way ANOVA and Tuckey’s post-hoc test.

Results: WKY rats showed significantly decreased number of rearings (by 70%, p<0.01),decreased number of total arm entries (by 21%, p<0.05) and the time spent in the open arms (by 73%, p<0.001) of the elevated plus-maze compared to WI control group. WKY females had a significantly lower level of BDNF in the hippocampus (by 12%, p<0.05) and mPFC (by 16%, p<0.05) and pAkt (by 14%, p<0.01) only in mPFC as compared to the WI female rats. Exposure of WKY females to CMS enhanced an anxiety-like behavior and hypolomotion (decrease in number of rearings by 31%, p<0.05, number of total arm entries by 89%, p<0.001, and timevspent in the open arms by 92%, p<0.001), further down-expression of BDNF in both brain areas (in PFC: by 15%, p<0.001; in hippocampus: by 7%, p<0.05) and Akt phosphorylation in the mPFC (by 17%, p<0.05) as well as a decreased pAkt in the hippocampus (by 36%, p<0.001).

Conclusions: The difference in the balance of BDNF and PI3K/Akt signaling pathways may be relevant to the resistance of WKY rats to antidepressant drug treatment and may be useful for developing new targets for depression treatment, especially in females.
C3  - Neuroscience Applied
T1  - Dysregulation of BDNF and PI3K/Akt signaling in the brain of female Wistar-Kyoto rats exposed to chronic mild stress
VL  - 2
IS  - Supplement 2
SP  - 102596
DO  - 10.1016/j.nsa.2023.102596
ER  - 

@conference{
author = "Virijević, Kristina and Spasojević, Nataša and Stefanović, Bojana and Ferizović, Harisa and Janković, Milica and Dronjak, Slađana",
year = "2023",
abstract = "Background: The neurobiology underlying depression has not yet been fully identified, but is thought to result from molecular and cellular abnormalities that interact with genetic and environmental factors. Depression is twice as prevalent in women as in men, however, females remain underrepresented in preclinical research. In addition to the neurotransmission theory of depression, the inflammatory processes and the disrupted signaling pathways also play a crucial role in the pathophysiology of depression. The WKY rat strain has long been established as a model of depression. These rats demonstrate an exaggerated response to stress compared to other strains. WKY strain fail to respond to chronic antidepressant treatment after exposure to chronic mild stress (CMS) and considered to be nonresponsive to antidepressant drugs. The hippocampus and the medial prefrontal cortex (mPFC) are thought to be an important regions for depression. Brain-derived neurotrophic factor (BDNF) play a vital role in the pathophysiology of depression. BDNF-stimulated signaling cascades, including the phosphatidylinositol 3-kinase (PI3K)–/serine threonine kinase (Akt), also implicated in depression and treatment respons. In the present study, we have examined the effects of CMS on behavior and BDNF and PI3K/Akt signaling in the hippocampus and mPFC of female WKY rats.

Method: In the experiment, we used three months old Wistar (WI) and WKY female rats. Animals were divided in two groups: control and animals exposed to CMS for 6 weeks. On the last day of stress procedure, animals were tested in elevated plus maze to determine the levels of anxiety. Animals were then sacrificed and hippocampus and mPFC were isolated. Levels of BDNF and pAkt were determined by Western blot method. Data were analyzed using the two way ANOVA and Tuckey’s post-hoc test.

Results: WKY rats showed significantly decreased number of rearings (by 70%, p<0.01),decreased number of total arm entries (by 21%, p<0.05) and the time spent in the open arms (by 73%, p<0.001) of the elevated plus-maze compared to WI control group. WKY females had a significantly lower level of BDNF in the hippocampus (by 12%, p<0.05) and mPFC (by 16%, p<0.05) and pAkt (by 14%, p<0.01) only in mPFC as compared to the WI female rats. Exposure of WKY females to CMS enhanced an anxiety-like behavior and hypolomotion (decrease in number of rearings by 31%, p<0.05, number of total arm entries by 89%, p<0.001, and timevspent in the open arms by 92%, p<0.001), further down-expression of BDNF in both brain areas (in PFC: by 15%, p<0.001; in hippocampus: by 7%, p<0.05) and Akt phosphorylation in the mPFC (by 17%, p<0.05) as well as a decreased pAkt in the hippocampus (by 36%, p<0.001).

Conclusions: The difference in the balance of BDNF and PI3K/Akt signaling pathways may be relevant to the resistance of WKY rats to antidepressant drug treatment and may be useful for developing new targets for depression treatment, especially in females.",
journal = "Neuroscience Applied",
title = "Dysregulation of BDNF and PI3K/Akt signaling in the brain of female Wistar-Kyoto rats exposed to chronic mild stress",
volume = "2",
number = "Supplement 2",
pages = "102596",
doi = "10.1016/j.nsa.2023.102596"
}

Virijević, K., Spasojević, N., Stefanović, B., Ferizović, H., Janković, M.,& Dronjak, S.. (2023). Dysregulation of BDNF and PI3K/Akt signaling in the brain of female Wistar-Kyoto rats exposed to chronic mild stress. in Neuroscience Applied, 2(Supplement 2), 102596.
https://doi.org/10.1016/j.nsa.2023.102596

Virijević K, Spasojević N, Stefanović B, Ferizović H, Janković M, Dronjak S. Dysregulation of BDNF and PI3K/Akt signaling in the brain of female Wistar-Kyoto rats exposed to chronic mild stress. in Neuroscience Applied. 2023;2(Supplement 2):102596.
doi:10.1016/j.nsa.2023.102596 .

Virijević, Kristina, Spasojević, Nataša, Stefanović, Bojana, Ferizović, Harisa, Janković, Milica, Dronjak, Slađana, "Dysregulation of BDNF and PI3K/Akt signaling in the brain of female Wistar-Kyoto rats exposed to chronic mild stress" in Neuroscience Applied, 2, no. Supplement 2 (2023):102596,
https://doi.org/10.1016/j.nsa.2023.102596 . .

TY  - JOUR
AU  - Ferizović, Harisa
AU  - Spasojević, Nataša
AU  - Puškaš, Nela
AU  - Stefanović, Bojana
AU  - Janković, Milica
AU  - Đelić, Ninoslav
AU  - Dronjak, Slađana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11385
AB  - Endocannabinoids act as a stress response system; simultaneously, the modulation of this system has emerged a novel approach for the therapy of cardiovascular disorders. We investigated the protective effects of the chronic administration of the fatty acid amide hydrolase inhibitor URB597 on morphology, pro-inflammatory and anti-inflammatory cytokine, the cytoplasm-nuclear distribution of JAK2/STAT3, and NF-κB and Nrf2/HO-1 signaling in the left ventricle of female and male rats exposed to chronic unpredictable stress. Our results show that URB597 treatment exhibits an antidepressant-like effect, decreases the heart/body weight ratio, prevents the hypertrophy of cardiomyocytes, and reduces the increased level of IL-6 in the wall of the left ventricle of stressed female and male rats. The phosphorylation levels of JAK2 and STAT3 in the ventricle of male rats treated with URB597 were declined, whereas in female rats the decrease of STAT3 was observed. In addition, URB597 reduced increased NF-κB in both females and males and increased the expression of Nrf2 and HO-1 protein in the cytosol of male rats, whereas did not affect their levels in females. Cardioprotective effects of URB597 could be linked to the ability to inhibit the JAK2 in males and the STAT3 inflammatory signaling pathways in both females and males.
T2  - Canadian Journal of Physiology and Pharmacology
T1  - URB597 attenuates stress-induced ventricular structural remodeling by modulating cytokines, NF-κB, and JAK2/STAT3 pathways in female and male rats
VL  - 101
IS  - 8
SP  - 400
EP  - 412
DO  - 10.1139/cjpp-2022-0535
ER  - 

@article{
author = "Ferizović, Harisa and Spasojević, Nataša and Puškaš, Nela and Stefanović, Bojana and Janković, Milica and Đelić, Ninoslav and Dronjak, Slađana",
year = "2023",
abstract = "Endocannabinoids act as a stress response system; simultaneously, the modulation of this system has emerged a novel approach for the therapy of cardiovascular disorders. We investigated the protective effects of the chronic administration of the fatty acid amide hydrolase inhibitor URB597 on morphology, pro-inflammatory and anti-inflammatory cytokine, the cytoplasm-nuclear distribution of JAK2/STAT3, and NF-κB and Nrf2/HO-1 signaling in the left ventricle of female and male rats exposed to chronic unpredictable stress. Our results show that URB597 treatment exhibits an antidepressant-like effect, decreases the heart/body weight ratio, prevents the hypertrophy of cardiomyocytes, and reduces the increased level of IL-6 in the wall of the left ventricle of stressed female and male rats. The phosphorylation levels of JAK2 and STAT3 in the ventricle of male rats treated with URB597 were declined, whereas in female rats the decrease of STAT3 was observed. In addition, URB597 reduced increased NF-κB in both females and males and increased the expression of Nrf2 and HO-1 protein in the cytosol of male rats, whereas did not affect their levels in females. Cardioprotective effects of URB597 could be linked to the ability to inhibit the JAK2 in males and the STAT3 inflammatory signaling pathways in both females and males.",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "URB597 attenuates stress-induced ventricular structural remodeling by modulating cytokines, NF-κB, and JAK2/STAT3 pathways in female and male rats",
volume = "101",
number = "8",
pages = "400-412",
doi = "10.1139/cjpp-2022-0535"
}

Ferizović, H., Spasojević, N., Puškaš, N., Stefanović, B., Janković, M., Đelić, N.,& Dronjak, S.. (2023). URB597 attenuates stress-induced ventricular structural remodeling by modulating cytokines, NF-κB, and JAK2/STAT3 pathways in female and male rats. in Canadian Journal of Physiology and Pharmacology, 101(8), 400-412.
https://doi.org/10.1139/cjpp-2022-0535

Ferizović H, Spasojević N, Puškaš N, Stefanović B, Janković M, Đelić N, Dronjak S. URB597 attenuates stress-induced ventricular structural remodeling by modulating cytokines, NF-κB, and JAK2/STAT3 pathways in female and male rats. in Canadian Journal of Physiology and Pharmacology. 2023;101(8):400-412.
doi:10.1139/cjpp-2022-0535 .

Ferizović, Harisa, Spasojević, Nataša, Puškaš, Nela, Stefanović, Bojana, Janković, Milica, Đelić, Ninoslav, Dronjak, Slađana, "URB597 attenuates stress-induced ventricular structural remodeling by modulating cytokines, NF-κB, and JAK2/STAT3 pathways in female and male rats" in Canadian Journal of Physiology and Pharmacology, 101, no. 8 (2023):400-412,
https://doi.org/10.1139/cjpp-2022-0535 . .

TY  - JOUR
AU  - Ferizović, Harisa
AU  - Spasojević, Nataša
AU  - Janković, Milica
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13112
AB  - Introduction: The present study examined the effects of fatty acid amide hydrolase inhibitor URB597 on the level of plasma catecholamine and their content, synthesis, and degradation in the adrenal medulla of male and female rats subjected to chronic unpredictable stress (CUS). Material and Methods: Male and female Wistar rats were exposed to the 6 weeks of CUS and treated intraperitoneally with either 0.3 mg/kg/day of URB597 or vehicle in the last 2 weeks of stress protocol. Catecholamines’ plasma levels and catecholamines’ levels in adrenal medulla were examined using Elabscience ELISA kits. Western blot analysis was used to detect the protein in the medulla. Results: The results of our experiment showed that adrenal weights and catecholamine of unstressed control were higher in females and that CUS induced further enlargement of adrenal glands and catecholamine content and its synthesis compared to male rats. CUS caused an increase of plasma norepinephrine and depletion of norepinephrine content as well as unchanged synthesis and degradation of catecholamine in the adrenal medulla of male rats. URB597 reduced enlarged adrenals and catecholamine content and its synthesis in stressed female rats. URB597 reduces increased plasma norepinephrine and restores its content in the adrenal medulla, unchanging the expression of enzyme synthesis, while reduced protein levels of monoamine oxidase A in male rats are exposed to CUS. Discussion: Our results support the role of endocannabinoids as an antistress mechanism that inhibits elevated adrenomedullary activation and promotes its recovery to baseline in both male and female stressed rats.
T2  - Pharmacology
T1  - Effects of Fatty Acid Amide Hydroxylase Inhibitor URB597 on the Catecholaminergic Activity of the Adrenal Medulla in Stressed Male and Female Rats
VL  - 107
IS  - 1-2
SP  - 81
EP  - 89
DO  - 10.1159/000519332
ER  - 

@article{
author = "Ferizović, Harisa and Spasojević, Nataša and Janković, Milica and Stefanović, Bojana and Dronjak, Slađana",
year = "2022",
abstract = "Introduction: The present study examined the effects of fatty acid amide hydrolase inhibitor URB597 on the level of plasma catecholamine and their content, synthesis, and degradation in the adrenal medulla of male and female rats subjected to chronic unpredictable stress (CUS). Material and Methods: Male and female Wistar rats were exposed to the 6 weeks of CUS and treated intraperitoneally with either 0.3 mg/kg/day of URB597 or vehicle in the last 2 weeks of stress protocol. Catecholamines’ plasma levels and catecholamines’ levels in adrenal medulla were examined using Elabscience ELISA kits. Western blot analysis was used to detect the protein in the medulla. Results: The results of our experiment showed that adrenal weights and catecholamine of unstressed control were higher in females and that CUS induced further enlargement of adrenal glands and catecholamine content and its synthesis compared to male rats. CUS caused an increase of plasma norepinephrine and depletion of norepinephrine content as well as unchanged synthesis and degradation of catecholamine in the adrenal medulla of male rats. URB597 reduced enlarged adrenals and catecholamine content and its synthesis in stressed female rats. URB597 reduces increased plasma norepinephrine and restores its content in the adrenal medulla, unchanging the expression of enzyme synthesis, while reduced protein levels of monoamine oxidase A in male rats are exposed to CUS. Discussion: Our results support the role of endocannabinoids as an antistress mechanism that inhibits elevated adrenomedullary activation and promotes its recovery to baseline in both male and female stressed rats.",
journal = "Pharmacology",
title = "Effects of Fatty Acid Amide Hydroxylase Inhibitor URB597 on the Catecholaminergic Activity of the Adrenal Medulla in Stressed Male and Female Rats",
volume = "107",
number = "1-2",
pages = "81-89",
doi = "10.1159/000519332"
}

Ferizović, H., Spasojević, N., Janković, M., Stefanović, B.,& Dronjak, S.. (2022). Effects of Fatty Acid Amide Hydroxylase Inhibitor URB597 on the Catecholaminergic Activity of the Adrenal Medulla in Stressed Male and Female Rats. in Pharmacology, 107(1-2), 81-89.
https://doi.org/10.1159/000519332

Ferizović H, Spasojević N, Janković M, Stefanović B, Dronjak S. Effects of Fatty Acid Amide Hydroxylase Inhibitor URB597 on the Catecholaminergic Activity of the Adrenal Medulla in Stressed Male and Female Rats. in Pharmacology. 2022;107(1-2):81-89.
doi:10.1159/000519332 .

Ferizović, Harisa, Spasojević, Nataša, Janković, Milica, Stefanović, Bojana, Dronjak, Slađana, "Effects of Fatty Acid Amide Hydroxylase Inhibitor URB597 on the Catecholaminergic Activity of the Adrenal Medulla in Stressed Male and Female Rats" in Pharmacology, 107, no. 1-2 (2022):81-89,
https://doi.org/10.1159/000519332 . .

TY  - CONF
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Ferizović, Harisa
AU  - Janković, Milica
AU  - Vasiljević, Perica
AU  - Dronjak, Slađana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12506
AB  - Istraživanja iz oblasti depresivnih poremećaja su sve više usmerena ka hipotezi da su oksidativni stres i inflamatorni procesi takođe uključeni u patogenezu depresije. Signalni put koji uključuje janus kinazu 2 (JAK2) i pretvarač signala i aktivator transkripcije 3 (STAT3) je povezan sa inflamacijom, a komponente ovog puta se eksprimiraju u različitim regionima mozga, posebno u hipokampusu. Cilj ovog rada bio je da se ispita uloga elemenata JAK2/STAT3 signalnog puta kod depresije izazvane hroničnim blagim stresom (CMS). U radu su korišćene ženke Wistar (W) i Wistar-Kyoto (WK) soja pacova. WK soj pokazuje neurondokrine promene, kao i promene u ponašanju slične depresiji, zbog čega se smatraju dobrim animalnim modelom endogene depresije. Odrasle W i WK ženke bile su izložene CMS u trajanju od 6 nedelja. Nakon ovog perioda jedinke su žrtvovane i izolovani su hipokampusi. Kvantitativna Western blot analiza naših rezultata je pokazala da je kod kontrolne WK grupe, u odnosu na kontrolnu W grupu, fosforilacija JAK2 i STAT3 u hipokampusu povećana, što ukazuje da je disregulacija ovog signalnog puta jedan od mogućih uzročnika simptoma depresije kod ovog soja. Hronični stres je dodatno povećao ekspresiju fosforilisane forme ovih proteina kod oba soja. Ova studija pružila je nove dokaze o učešću JAK2/STAT3 signalnog puta u patogenezi depresije i može nas usmeriti u nalaženju novih, efikasnijih terapija u lečenju rezistentne depresije.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka
T1  - Disregulacija JAK2/STAT3 signalnog puta u hipokampusu pacova sa genetski predisponiranim depresivnim ponašanjem
SP  - 359
EP  - 359
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12506
ER  - 

@conference{
author = "Stefanović, Bojana and Spasojević, Nataša and Ferizović, Harisa and Janković, Milica and Vasiljević, Perica and Dronjak, Slađana",
year = "2022",
abstract = "Istraživanja iz oblasti depresivnih poremećaja su sve više usmerena ka hipotezi da su oksidativni stres i inflamatorni procesi takođe uključeni u patogenezu depresije. Signalni put koji uključuje janus kinazu 2 (JAK2) i pretvarač signala i aktivator transkripcije 3 (STAT3) je povezan sa inflamacijom, a komponente ovog puta se eksprimiraju u različitim regionima mozga, posebno u hipokampusu. Cilj ovog rada bio je da se ispita uloga elemenata JAK2/STAT3 signalnog puta kod depresije izazvane hroničnim blagim stresom (CMS). U radu su korišćene ženke Wistar (W) i Wistar-Kyoto (WK) soja pacova. WK soj pokazuje neurondokrine promene, kao i promene u ponašanju slične depresiji, zbog čega se smatraju dobrim animalnim modelom endogene depresije. Odrasle W i WK ženke bile su izložene CMS u trajanju od 6 nedelja. Nakon ovog perioda jedinke su žrtvovane i izolovani su hipokampusi. Kvantitativna Western blot analiza naših rezultata je pokazala da je kod kontrolne WK grupe, u odnosu na kontrolnu W grupu, fosforilacija JAK2 i STAT3 u hipokampusu povećana, što ukazuje da je disregulacija ovog signalnog puta jedan od mogućih uzročnika simptoma depresije kod ovog soja. Hronični stres je dodatno povećao ekspresiju fosforilisane forme ovih proteina kod oba soja. Ova studija pružila je nove dokaze o učešću JAK2/STAT3 signalnog puta u patogenezi depresije i može nas usmeriti u nalaženju novih, efikasnijih terapija u lečenju rezistentne depresije.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka",
title = "Disregulacija JAK2/STAT3 signalnog puta u hipokampusu pacova sa genetski predisponiranim depresivnim ponašanjem",
pages = "359-359",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12506"
}

Stefanović, B., Spasojević, N., Ferizović, H., Janković, M., Vasiljević, P.,& Dronjak, S.. (2022). Disregulacija JAK2/STAT3 signalnog puta u hipokampusu pacova sa genetski predisponiranim depresivnim ponašanjem. in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka
Beograd : Srpsko biološko društvo., 359-359.
https://hdl.handle.net/21.15107/rcub_vinar_12506

Stefanović B, Spasojević N, Ferizović H, Janković M, Vasiljević P, Dronjak S. Disregulacija JAK2/STAT3 signalnog puta u hipokampusu pacova sa genetski predisponiranim depresivnim ponašanjem. in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka. 2022;:359-359.
https://hdl.handle.net/21.15107/rcub_vinar_12506 .

Stefanović, Bojana, Spasojević, Nataša, Ferizović, Harisa, Janković, Milica, Vasiljević, Perica, Dronjak, Slađana, "Disregulacija JAK2/STAT3 signalnog puta u hipokampusu pacova sa genetski predisponiranim depresivnim ponašanjem" in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka (2022):359-359,
https://hdl.handle.net/21.15107/rcub_vinar_12506 .

TY  - CONF
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Ferizović, Harisa
AU  - Janković, Milica
AU  - Dronjak, Slađana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12505
AB  - Brojni dokazi ukazuju da socijalna izolacija može imati dugoročne efekte na ponašanje i u odgovoru na stres. Wistar-Kyoto (WK) soj pacova se smatra dobrim animalnim modelom rezistentne depresije jer su, za razliku od Wistar (W) soja, nakon izlaganja stresu neosetljivi na terapiju antidepresivima. U ovoj studiji smo izlagali ženke WK i W soja pacova hroničnom blagom stresu (CMS) u trajanju od 6 nedelja. Kontrolne životinje su bile u grupi po 3 jedinke, dok je CMS grupa pacova bila izolovana 3 nedelje pre početka CMS procedure do kraja eksperimenta. Znaci anhedonije su tokom 6 nedelja bili procenjivani testom unosa saharoze, dok je stepen anksioznosti utvrđen testom izdignutog plus lavirinta na kraju eksperimenta. Kontrolna WK grupa je pokazivala od prve do poslednje nedelje eksperimenta smanjen unos saharoze, hipolokomociju i smanjen broj ulaza u otvorene krake u odnosu na kontrolnu W grupu, što potvrđuje da ovaj soj i u odsustvu stresa pokazuje simptome depresije i anksioznosti. Izlaganje CMS je dovelo do dodatnih razlika između sojeva u unosu saharoze. Izolacija u trajanju od 3 nedelje pre CMS dovela je kod oba soja do povećanja unosa saharoze, što može ukazati na povećanu potrebu za zadovoljstvima. Hronični blagi stres je samo kod W soja doveo do znakova anhedonije, dok su ženke WK soja i nakon 6 nedelja CMS imale povećan unos saharoze. Ovi rezultati su relevantni za razumevanje načina na koji socijalna izolacija doprinosi osetljivosti pojedinca na poremećaje povezane sa stresom tokom života.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka
T1  - Uticaj hroničnog blagog stresa na ponašanje Wistar-Kyoto soja pacova kao animalnog modela rezistentne depresije
SP  - 380
EP  - 380
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12505
ER  - 

@conference{
author = "Spasojević, Nataša and Stefanović, Bojana and Ferizović, Harisa and Janković, Milica and Dronjak, Slađana",
year = "2022",
abstract = "Brojni dokazi ukazuju da socijalna izolacija može imati dugoročne efekte na ponašanje i u odgovoru na stres. Wistar-Kyoto (WK) soj pacova se smatra dobrim animalnim modelom rezistentne depresije jer su, za razliku od Wistar (W) soja, nakon izlaganja stresu neosetljivi na terapiju antidepresivima. U ovoj studiji smo izlagali ženke WK i W soja pacova hroničnom blagom stresu (CMS) u trajanju od 6 nedelja. Kontrolne životinje su bile u grupi po 3 jedinke, dok je CMS grupa pacova bila izolovana 3 nedelje pre početka CMS procedure do kraja eksperimenta. Znaci anhedonije su tokom 6 nedelja bili procenjivani testom unosa saharoze, dok je stepen anksioznosti utvrđen testom izdignutog plus lavirinta na kraju eksperimenta. Kontrolna WK grupa je pokazivala od prve do poslednje nedelje eksperimenta smanjen unos saharoze, hipolokomociju i smanjen broj ulaza u otvorene krake u odnosu na kontrolnu W grupu, što potvrđuje da ovaj soj i u odsustvu stresa pokazuje simptome depresije i anksioznosti. Izlaganje CMS je dovelo do dodatnih razlika između sojeva u unosu saharoze. Izolacija u trajanju od 3 nedelje pre CMS dovela je kod oba soja do povećanja unosa saharoze, što može ukazati na povećanu potrebu za zadovoljstvima. Hronični blagi stres je samo kod W soja doveo do znakova anhedonije, dok su ženke WK soja i nakon 6 nedelja CMS imale povećan unos saharoze. Ovi rezultati su relevantni za razumevanje načina na koji socijalna izolacija doprinosi osetljivosti pojedinca na poremećaje povezane sa stresom tokom života.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka",
title = "Uticaj hroničnog blagog stresa na ponašanje Wistar-Kyoto soja pacova kao animalnog modela rezistentne depresije",
pages = "380-380",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12505"
}

Spasojević, N., Stefanović, B., Ferizović, H., Janković, M.,& Dronjak, S.. (2022). Uticaj hroničnog blagog stresa na ponašanje Wistar-Kyoto soja pacova kao animalnog modela rezistentne depresije. in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka
Beograd : Srpsko biološko društvo., 380-380.
https://hdl.handle.net/21.15107/rcub_vinar_12505

Spasojević N, Stefanović B, Ferizović H, Janković M, Dronjak S. Uticaj hroničnog blagog stresa na ponašanje Wistar-Kyoto soja pacova kao animalnog modela rezistentne depresije. in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka. 2022;:380-380.
https://hdl.handle.net/21.15107/rcub_vinar_12505 .

Spasojević, Nataša, Stefanović, Bojana, Ferizović, Harisa, Janković, Milica, Dronjak, Slađana, "Uticaj hroničnog blagog stresa na ponašanje Wistar-Kyoto soja pacova kao animalnog modela rezistentne depresije" in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka (2022):380-380,
https://hdl.handle.net/21.15107/rcub_vinar_12505 .

TY  - JOUR
AU  - Janković, Milica
AU  - Spasojević, Nataša
AU  - Ferizović, Harisa
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10056
AB  - An increasing body of evidence shows significant sex differences in the mammalian brain in multiple behaviours and psychiatric and neurological diseases and as well as that the endocannabinoid system may differ between males and females. In this study we investigated sex differences in working, short-term and long-term memory and the expression of β2-adrenergic and D1- and D2-receptors in the mPFC and hippocampus, brain regions that are involved in stress response and memory modulation in rats exposed to the chronic unpredictable stress (CUS) and the potential beneficial effects of the chronic fatty acid amide hydrolase inhibitor URB597 treatment. Chronically stressed male rats had an improvement of working memory, while stressed females showed very low object-recognition abilities. On the other hand, animals of both sexes exhibited long-term memory impairment. Our results showed that CUS decreased the expression of β2-adrenoceptors in the mPFC and D1 receptors in the mPFC and hippocampus of male rats and decreased β2-adrenoceptors and D1- receptors in the hippocampus of female. URB597 treatment had a positive effect on the short-term memory of stressed animals of both sexes whereas failed to restore long-term memory and did not affect the protein levels β2-adrenoceptors and D1 receptors in the hippocampus of CUS female rats. The present results support that endocannabinoids induced long-term memory and neurochemical alternations which are sex dependent, suggesting sex specific treatment strategies of mental disorders.
T2  - Neuroscience Letters
T1  - Sex specific effects of the fatty acid amide hydrolase inhibitor URB597 on memory and brain β2-adrenergic and D1-dopamine receptors
VL  - 768
SP  - 136363
DO  - 10.1016/j.neulet.2021.136363
ER  - 

@article{
author = "Janković, Milica and Spasojević, Nataša and Ferizović, Harisa and Stefanović, Bojana and Dronjak, Slađana",
year = "2022",
abstract = "An increasing body of evidence shows significant sex differences in the mammalian brain in multiple behaviours and psychiatric and neurological diseases and as well as that the endocannabinoid system may differ between males and females. In this study we investigated sex differences in working, short-term and long-term memory and the expression of β2-adrenergic and D1- and D2-receptors in the mPFC and hippocampus, brain regions that are involved in stress response and memory modulation in rats exposed to the chronic unpredictable stress (CUS) and the potential beneficial effects of the chronic fatty acid amide hydrolase inhibitor URB597 treatment. Chronically stressed male rats had an improvement of working memory, while stressed females showed very low object-recognition abilities. On the other hand, animals of both sexes exhibited long-term memory impairment. Our results showed that CUS decreased the expression of β2-adrenoceptors in the mPFC and D1 receptors in the mPFC and hippocampus of male rats and decreased β2-adrenoceptors and D1- receptors in the hippocampus of female. URB597 treatment had a positive effect on the short-term memory of stressed animals of both sexes whereas failed to restore long-term memory and did not affect the protein levels β2-adrenoceptors and D1 receptors in the hippocampus of CUS female rats. The present results support that endocannabinoids induced long-term memory and neurochemical alternations which are sex dependent, suggesting sex specific treatment strategies of mental disorders.",
journal = "Neuroscience Letters",
title = "Sex specific effects of the fatty acid amide hydrolase inhibitor URB597 on memory and brain β2-adrenergic and D1-dopamine receptors",
volume = "768",
pages = "136363",
doi = "10.1016/j.neulet.2021.136363"
}

Janković, M., Spasojević, N., Ferizović, H., Stefanović, B.,& Dronjak, S.. (2022). Sex specific effects of the fatty acid amide hydrolase inhibitor URB597 on memory and brain β2-adrenergic and D1-dopamine receptors. in Neuroscience Letters, 768, 136363.
https://doi.org/10.1016/j.neulet.2021.136363

Janković M, Spasojević N, Ferizović H, Stefanović B, Dronjak S. Sex specific effects of the fatty acid amide hydrolase inhibitor URB597 on memory and brain β2-adrenergic and D1-dopamine receptors. in Neuroscience Letters. 2022;768:136363.
doi:10.1016/j.neulet.2021.136363 .

Janković, Milica, Spasojević, Nataša, Ferizović, Harisa, Stefanović, Bojana, Dronjak, Slađana, "Sex specific effects of the fatty acid amide hydrolase inhibitor URB597 on memory and brain β2-adrenergic and D1-dopamine receptors" in Neuroscience Letters, 768 (2022):136363,
https://doi.org/10.1016/j.neulet.2021.136363 . .

TY  - JOUR
AU  - Ferizović, Harisa
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Janković, Milica
AU  - Dronjak, Slađana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9007
AB  - The changes in sympathetic innervations in lymphoid organs could be a key factor in immune dysregulation. The endocannabinoid system has been shown to exhibit potent immunomodulatory effects that may differ between males and females, representing a potential therapeutic target for peripheral and central inflammatory disorders. Thus, in the present study, an examination was made of the effect of fatty acid amide hydrolase inhibitor URB597 treatment on splenic catecholamine content, synthesis, uptake and degradation in chronically unpredictably stressed (CUS) female and male rats. The results show that CUS increases anxiety-like behaviors and that URB597 had an anxiolytic effect on chronically stressed animals of both sexes. CUS induced the expression of plasma interleukin – 6 (IL-6), interleukin – 10 (IL-10) and IL-6 in the spleen, whereas the expression of IL-10 was reduced in the spleen of both sexes. URB597 treatment did not cause changes in IL-6 in plasma or the spleen, whereas it increased IL-10 in the spleen in CUS animals of both sexes. CUS caused a significant depletion of noradrenaline content in the spleen of female rats and a reduction in noradrenaline uptake in the spleen of female rats, while stressed males had a small but insignificant decrease of splenic noradrenaline levels and an enhanced uptake. The FAAH inhibitor URB597 enhances reduced noradrenaline content, affecting its uptake directly at the level of the spleen. It gives rise to the possibility that endocannabinoids exert a neurorestorative effect on the sympathetic nerve system and cell-mediated immune responses in the spleen of chronically stressed rats.
T2  - International Immunopharmacology
T1  - The fatty acid amide hydrolase inhibitor URB597 modulates splenic catecholamines in chronically stressed female and male rats
VL  - 85
SP  - 106615
DO  - 10.1016/j.intimp.2020.106615
ER  - 

@article{
author = "Ferizović, Harisa and Spasojević, Nataša and Stefanović, Bojana and Janković, Milica and Dronjak, Slađana",
year = "2020",
abstract = "The changes in sympathetic innervations in lymphoid organs could be a key factor in immune dysregulation. The endocannabinoid system has been shown to exhibit potent immunomodulatory effects that may differ between males and females, representing a potential therapeutic target for peripheral and central inflammatory disorders. Thus, in the present study, an examination was made of the effect of fatty acid amide hydrolase inhibitor URB597 treatment on splenic catecholamine content, synthesis, uptake and degradation in chronically unpredictably stressed (CUS) female and male rats. The results show that CUS increases anxiety-like behaviors and that URB597 had an anxiolytic effect on chronically stressed animals of both sexes. CUS induced the expression of plasma interleukin – 6 (IL-6), interleukin – 10 (IL-10) and IL-6 in the spleen, whereas the expression of IL-10 was reduced in the spleen of both sexes. URB597 treatment did not cause changes in IL-6 in plasma or the spleen, whereas it increased IL-10 in the spleen in CUS animals of both sexes. CUS caused a significant depletion of noradrenaline content in the spleen of female rats and a reduction in noradrenaline uptake in the spleen of female rats, while stressed males had a small but insignificant decrease of splenic noradrenaline levels and an enhanced uptake. The FAAH inhibitor URB597 enhances reduced noradrenaline content, affecting its uptake directly at the level of the spleen. It gives rise to the possibility that endocannabinoids exert a neurorestorative effect on the sympathetic nerve system and cell-mediated immune responses in the spleen of chronically stressed rats.",
journal = "International Immunopharmacology",
title = "The fatty acid amide hydrolase inhibitor URB597 modulates splenic catecholamines in chronically stressed female and male rats",
volume = "85",
pages = "106615",
doi = "10.1016/j.intimp.2020.106615"
}

Ferizović, H., Spasojević, N., Stefanović, B., Janković, M.,& Dronjak, S.. (2020). The fatty acid amide hydrolase inhibitor URB597 modulates splenic catecholamines in chronically stressed female and male rats. in International Immunopharmacology, 85, 106615.
https://doi.org/10.1016/j.intimp.2020.106615

Ferizović H, Spasojević N, Stefanović B, Janković M, Dronjak S. The fatty acid amide hydrolase inhibitor URB597 modulates splenic catecholamines in chronically stressed female and male rats. in International Immunopharmacology. 2020;85:106615.
doi:10.1016/j.intimp.2020.106615 .

Ferizović, Harisa, Spasojević, Nataša, Stefanović, Bojana, Janković, Milica, Dronjak, Slađana, "The fatty acid amide hydrolase inhibitor URB597 modulates splenic catecholamines in chronically stressed female and male rats" in International Immunopharmacology, 85 (2020):106615,
https://doi.org/10.1016/j.intimp.2020.106615 . .

TY  - CONF
AU  - Ferizović, Harisa
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12813
AB  - Disrupted circadian rhythm and sleep disorders are one of the crucial symptoms of major depressive disorder. While physiological role of melatonin and its antioxidative properties are well known, increasing number of studies show that melatonin may also exhibit antidepressive effects. Aim of our research was to examine effects of melatonin on catecholamine turnover and receptors in prefrontal cortex (PFC) of rats exposed to the chronic mild unpredictable stress (CUMS) model of depression. Two months old Wistar rats were subjected to the 4 weeks of CUMS. Both control animals and animals exposed to the CUMS were treated with either melatonin (10 mg/kg, i.p.) or vehicle. Levels of tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), catechol-O-methyltransferase (COMT), α1- and β2-adrenergic receptors in PFC were examined using Western blot procedure. Catecholamine synthesis through TH and DBH in PFC was not affected either by CUMS procedure or by melatonin treatment. However, animals exposed to the CUMS shoved elevated levels of COMT and decreased levels of α1- and β2-adrenergic receptors in PFC. Melatonin treatment improved levels of adrenergic receptors and decreased levels of COMT protein to the levels comparable with control animals. These findings show that melatonin treatment had protective role on PFC in chronic stress. Our results suggest that some of the melatonin effects were achieved through regulation of COMT and α1- and β2-adrenergic receptors protein levels. These preliminary results support potential therapeutic use of melatonin in treatment of depressive disorders.
PB  - Beograd : Srpsko farmakološko društvo (SFD)
C3  - 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova
T1  - Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu
T1  - Effects of melatonin treatment on catecholamine synthesis, degradation and α1-and β2-adrenergic receptors in prefrontal cortex of rats exposed to the chronic mild unpredictable stress
SP  - 206
EP  - 207
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12813
ER  - 

@conference{
author = "Ferizović, Harisa and Spasojević, Nataša and Stefanović, Bojana and Dronjak, Slađana",
year = "2019",
abstract = "Disrupted circadian rhythm and sleep disorders are one of the crucial symptoms of major depressive disorder. While physiological role of melatonin and its antioxidative properties are well known, increasing number of studies show that melatonin may also exhibit antidepressive effects. Aim of our research was to examine effects of melatonin on catecholamine turnover and receptors in prefrontal cortex (PFC) of rats exposed to the chronic mild unpredictable stress (CUMS) model of depression. Two months old Wistar rats were subjected to the 4 weeks of CUMS. Both control animals and animals exposed to the CUMS were treated with either melatonin (10 mg/kg, i.p.) or vehicle. Levels of tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), catechol-O-methyltransferase (COMT), α1- and β2-adrenergic receptors in PFC were examined using Western blot procedure. Catecholamine synthesis through TH and DBH in PFC was not affected either by CUMS procedure or by melatonin treatment. However, animals exposed to the CUMS shoved elevated levels of COMT and decreased levels of α1- and β2-adrenergic receptors in PFC. Melatonin treatment improved levels of adrenergic receptors and decreased levels of COMT protein to the levels comparable with control animals. These findings show that melatonin treatment had protective role on PFC in chronic stress. Our results suggest that some of the melatonin effects were achieved through regulation of COMT and α1- and β2-adrenergic receptors protein levels. These preliminary results support potential therapeutic use of melatonin in treatment of depressive disorders.",
publisher = "Beograd : Srpsko farmakološko društvo (SFD)",
journal = "14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova",
title = "Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu, Effects of melatonin treatment on catecholamine synthesis, degradation and α1-and β2-adrenergic receptors in prefrontal cortex of rats exposed to the chronic mild unpredictable stress",
pages = "206-207",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12813"
}

Ferizović, H., Spasojević, N., Stefanović, B.,& Dronjak, S.. (2019). Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu. in 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova
Beograd : Srpsko farmakološko društvo (SFD)., 206-207.
https://hdl.handle.net/21.15107/rcub_vinar_12813

Ferizović H, Spasojević N, Stefanović B, Dronjak S. Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu. in 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova. 2019;:206-207.
https://hdl.handle.net/21.15107/rcub_vinar_12813 .

Ferizović, Harisa, Spasojević, Nataša, Stefanović, Bojana, Dronjak, Slađana, "Efekat tretmana melatoninom na sintezu I degradaciju kateholamina i α1- i β2-adrenergičke receptore u prefrontalnoj kori pacova izloženih hroničnom blagom nepredvidivom stresu" in 14. Kongres farmakologa Srbije i 4. Kongres kliničke farmakologije Srbije sa međunarodnim učešćem : Zbornik kratkih sadržaja radova (2019):206-207,
https://hdl.handle.net/21.15107/rcub_vinar_12813 .

TY  - JOUR
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8361
AB  - This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press.
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression
VL  - 97
IS  - 7
SP  - 685
EP  - 690
DO  - 10.1139/cjpp-2018-0612
ER  - 

@article{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana",
year = "2019",
abstract = "This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press.",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression",
volume = "97",
number = "7",
pages = "685-690",
doi = "10.1139/cjpp-2018-0612"
}

Stefanović, B., Spasojević, N., Jovanović, P.,& Dronjak, S.. (2019). Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. in Canadian Journal of Physiology and Pharmacology, 97(7), 685-690.
https://doi.org/10.1139/cjpp-2018-0612

Stefanović B, Spasojević N, Jovanović P, Dronjak S. Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. in Canadian Journal of Physiology and Pharmacology. 2019;97(7):685-690.
doi:10.1139/cjpp-2018-0612 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression" in Canadian Journal of Physiology and Pharmacology, 97, no. 7 (2019):685-690,
https://doi.org/10.1139/cjpp-2018-0612 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Spasojević, Nataša
AU  - Puškaš, Nela
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8400
AB  - Social stress produces behavioral alterations, and autonomic and cardiac dysfunction in animals. In addition to the well-known roles of oxytocin on birth and maternal bonding, recent evidence shows that this neuropeptide possesses cardio-protective properties. However less is known about its role in the regulation of the autonomic nervous system. The direct influence of oxytocin on the cardiac catecholamine synthesizing enzyme, transport beta-adrenoceptors and muscarinic receptors in animals exposed to chronic social isolation stress has not yet been studied. In this study, we examined the influence of peripheral chronic oxytocin treatment on anxiety-related behavior, the morphology and content of epinephrine and norepinephrine, mRNA and protein levels of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and receptors <beta> 3 (β3-AR) and muscarinic 2 (M2 MR) in the right and left cardiac atrium and ventricle of chronically socially isolated male rats. Our results show that oxytocin treatment exhibits an anxiolytic effect, decreases the heart/body weight ratio and prevents the hypertrophy of cardiomyocytes in the wall of the left ventricle of stressed rats. Epinephrine and TH protein levels were unchanged after prolonged oxytocin treatment. Peripheral oxytocin administration led to the enhancement of gene expression of β3-AR in both atria, NET protein in the left ventricle and gene expression of M2 MR in the right atrium and the left ventricle of chronically socially isolated rats. The study provides evidence that oxytocin treatment in chronically socially isolated animals enhances norepinephrine uptake and expression of cardio-inhibitory receptors in cardiac tissues, which could have a beneficial effect on the cardiovascular system under the increased activity of the sympathoneural system. © 2018 Elsevier Inc.
T2  - Peptides
T1  - Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats
VL  - 111
SP  - 132
EP  - 141
DO  - 10.1016/j.peptides.2018.06.008
ER  - 

@article{
author = "Jovanović, Predrag and Spasojević, Nataša and Puškaš, Nela and Stefanović, Bojana and Dronjak, Slađana",
year = "2019",
abstract = "Social stress produces behavioral alterations, and autonomic and cardiac dysfunction in animals. In addition to the well-known roles of oxytocin on birth and maternal bonding, recent evidence shows that this neuropeptide possesses cardio-protective properties. However less is known about its role in the regulation of the autonomic nervous system. The direct influence of oxytocin on the cardiac catecholamine synthesizing enzyme, transport beta-adrenoceptors and muscarinic receptors in animals exposed to chronic social isolation stress has not yet been studied. In this study, we examined the influence of peripheral chronic oxytocin treatment on anxiety-related behavior, the morphology and content of epinephrine and norepinephrine, mRNA and protein levels of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and receptors <beta> 3 (β3-AR) and muscarinic 2 (M2 MR) in the right and left cardiac atrium and ventricle of chronically socially isolated male rats. Our results show that oxytocin treatment exhibits an anxiolytic effect, decreases the heart/body weight ratio and prevents the hypertrophy of cardiomyocytes in the wall of the left ventricle of stressed rats. Epinephrine and TH protein levels were unchanged after prolonged oxytocin treatment. Peripheral oxytocin administration led to the enhancement of gene expression of β3-AR in both atria, NET protein in the left ventricle and gene expression of M2 MR in the right atrium and the left ventricle of chronically socially isolated rats. The study provides evidence that oxytocin treatment in chronically socially isolated animals enhances norepinephrine uptake and expression of cardio-inhibitory receptors in cardiac tissues, which could have a beneficial effect on the cardiovascular system under the increased activity of the sympathoneural system. © 2018 Elsevier Inc.",
journal = "Peptides",
title = "Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats",
volume = "111",
pages = "132-141",
doi = "10.1016/j.peptides.2018.06.008"
}

Jovanović, P., Spasojević, N., Puškaš, N., Stefanović, B.,& Dronjak, S.. (2019). Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats. in Peptides, 111, 132-141.
https://doi.org/10.1016/j.peptides.2018.06.008

Jovanović P, Spasojević N, Puškaš N, Stefanović B, Dronjak S. Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats. in Peptides. 2019;111:132-141.
doi:10.1016/j.peptides.2018.06.008 .

Jovanović, Predrag, Spasojević, Nataša, Puškaš, Nela, Stefanović, Bojana, Dronjak, Slađana, "Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats" in Peptides, 111 (2019):132-141,
https://doi.org/10.1016/j.peptides.2018.06.008 . .

TY  - JOUR
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8410
AB  - This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press.
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression
VL  - 97
IS  - 7
SP  - 685
EP  - 690
DO  - 10.1139/cjpp-2018-0612
ER  - 

@article{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana",
year = "2019",
abstract = "This study investigated the effects of melatonin treatment on adrenal catecholamine content, synthesis, uptake, and vesicular transport induced by the chronic unpredictable mild stress (CUMS) model of depression in rats. This entailed quantifying the norepinephrine, epinephrine, mRNA, and protein levels of tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT), norepinephrine transporter (NET), and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. CUMS caused a significant depletion of norepinephrine stores and protein levels of TH, DBH, and NET, whereas the gene expression of PNMT was increased. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in norepinephrine content and the protein expression of TH, DBH, and NET in the adrenal medulla of chronically stressed rats. The present study demonstrates the stimulatory effect of melatonin on adrenomedullary synthesis, the uptake and content of catecholamine in the rat model of chronic stress-induced depression. © 2019, Published by NRC Research Press.",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression",
volume = "97",
number = "7",
pages = "685-690",
doi = "10.1139/cjpp-2018-0612"
}

Stefanović, B., Spasojević, N., Jovanović, P.,& Dronjak, S.. (2019). Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. in Canadian Journal of Physiology and Pharmacology, 97(7), 685-690.
https://doi.org/10.1139/cjpp-2018-0612

Stefanović B, Spasojević N, Jovanović P, Dronjak S. Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression. in Canadian Journal of Physiology and Pharmacology. 2019;97(7):685-690.
doi:10.1139/cjpp-2018-0612 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Melatonin treatment affects changes in adrenal gene expression of catecholamine biosynthesizing enzymes and norepinephrine transporter in the rat model of chronic-stress-induced depression" in Canadian Journal of Physiology and Pharmacology, 97, no. 7 (2019):685-690,
https://doi.org/10.1139/cjpp-2018-0612 . .

TY  - JOUR
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Ferizović, Harisa
AU  - Dronjak, Slađana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7795
AB  - OBJECTIVE: This study investigated the effects of chronic melatonin treatment on gene expression of α1-, α2-, β1- and β2-adrenoceptors in the hippocampus of rats subjected to chronic unpredictable mild stress (CUMS). BACKGROUND: Preclinical studies have also shown that melatonin prevented short- and long-term memory impairments and exhibited antidepressant-like actions. METHODS: For this study, we used 24 animals, which were divided into four groups, and the experiment lasted 4 weeks. We quantifi ed the changes in mRNA and protein levels of α1-, α2-, β1- and β2-adrenoceptors in the hippocampus after melatonin treatment. RESULTS: Our results demonstrated a decreased gene expression of α1-, α2- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress, while there was no change in gene expression of β1-adrenoceptors. Melatonin treatment in the CUMS rats prevented the stress-induced decrease in mRNA and protein levels of α1-and β2-adrenoceptors, whereas did not affect either on mRNA or protein level of β1-and α2-adrenoceptors. CONCLUSION: Our data suggest that melatonin, by increasing reduced levels of α1- and β2-adrenoceptors mRNA and protein in the hippocampus of chronic stressed rats, may be benefi cial in conditions such as chronic stress and provides an experimental opportunity to probe into further molecular mechanisms underlying the regulation of these receptor subtype.
T2  - Bratislava Medical Journal
T1  - Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress
VL  - 119
IS  - 7
SP  - 429
EP  - 433
DO  - 10.4149/BLL_2018_078
ER  - 

@article{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Ferizović, Harisa and Dronjak, Slađana",
year = "2018",
abstract = "OBJECTIVE: This study investigated the effects of chronic melatonin treatment on gene expression of α1-, α2-, β1- and β2-adrenoceptors in the hippocampus of rats subjected to chronic unpredictable mild stress (CUMS). BACKGROUND: Preclinical studies have also shown that melatonin prevented short- and long-term memory impairments and exhibited antidepressant-like actions. METHODS: For this study, we used 24 animals, which were divided into four groups, and the experiment lasted 4 weeks. We quantifi ed the changes in mRNA and protein levels of α1-, α2-, β1- and β2-adrenoceptors in the hippocampus after melatonin treatment. RESULTS: Our results demonstrated a decreased gene expression of α1-, α2- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress, while there was no change in gene expression of β1-adrenoceptors. Melatonin treatment in the CUMS rats prevented the stress-induced decrease in mRNA and protein levels of α1-and β2-adrenoceptors, whereas did not affect either on mRNA or protein level of β1-and α2-adrenoceptors. CONCLUSION: Our data suggest that melatonin, by increasing reduced levels of α1- and β2-adrenoceptors mRNA and protein in the hippocampus of chronic stressed rats, may be benefi cial in conditions such as chronic stress and provides an experimental opportunity to probe into further molecular mechanisms underlying the regulation of these receptor subtype.",
journal = "Bratislava Medical Journal",
title = "Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress",
volume = "119",
number = "7",
pages = "429-433",
doi = "10.4149/BLL_2018_078"
}

Stefanović, B., Spasojević, N., Jovanović, P., Ferizović, H.,& Dronjak, S.. (2018). Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress. in Bratislava Medical Journal, 119(7), 429-433.
https://doi.org/10.4149/BLL_2018_078

Stefanović B, Spasojević N, Jovanović P, Ferizović H, Dronjak S. Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress. in Bratislava Medical Journal. 2018;119(7):429-433.
doi:10.4149/BLL_2018_078 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Ferizović, Harisa, Dronjak, Slađana, "Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress" in Bratislava Medical Journal, 119, no. 7 (2018):429-433,
https://doi.org/10.4149/BLL_2018_078 . .

TY  - CONF
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12289
AB  - Introduction: Stress is considered a determinant in the etiology of depression. The adrenal medulla plays a key role in response to stress by releasing catecholamines, which are important to maintain homeostasis. Many studies have assessed the antidepressant-like activity of the melatonin, a neurohormone synthesized in the pineal gland. Thus, in the present study, we examined the effect of chronic melatonin treatment on mRNA levels and protein content of catecholamines biosynthetic enzymes (TH, DBH and PNMT) and transporters (NET, VMAT2) in adrenal medulla of rats exposed to chronic unpredictable mild stress (CUMS). Material and Methods: CUMS was used as an animal model of depression. Exposure of rats to CUMS and placebo or melatonin (10 mg/kg body weight, i.p.) administration started on the same day and was continued for 4 weeks. For quantifying TH, DBH, PNMT, NET and VMAT2 mRNA and protein levels we used realtime PCR and Western blot analysis. Results: We observed that CUMS induced increased mRNA levels of catecholamine biosynthetic enzymes (TH, DBH, and PNMT), and noradrenaline transporter NET, while treatment with melatonin decreased these biosynthetic enzymes and transporter. Conversely, CUMS induced a decrease in protein content of TH and NET, while chronic melatonin treatment increased NET protein levels in both control and stressed rats. CUMS and melatonin treatment has no effect on mRNA levels and protein content of VMAT2. Conclusion: This study suggests that the observed decrease of catecholamine biosynthesis and enhanced re-uptake in adrenal medulla of rats exposed to CUMS are connected to the beneficial effects of chronic melatonin treatment.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Melatonin modulates catecholamine biosynthesis and re-uptake in adrenal medulla of rats exposed to chronic unpredictable mild stress
SP  - 77
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12289
ER  - 

@conference{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana",
year = "2017",
abstract = "Introduction: Stress is considered a determinant in the etiology of depression. The adrenal medulla plays a key role in response to stress by releasing catecholamines, which are important to maintain homeostasis. Many studies have assessed the antidepressant-like activity of the melatonin, a neurohormone synthesized in the pineal gland. Thus, in the present study, we examined the effect of chronic melatonin treatment on mRNA levels and protein content of catecholamines biosynthetic enzymes (TH, DBH and PNMT) and transporters (NET, VMAT2) in adrenal medulla of rats exposed to chronic unpredictable mild stress (CUMS). Material and Methods: CUMS was used as an animal model of depression. Exposure of rats to CUMS and placebo or melatonin (10 mg/kg body weight, i.p.) administration started on the same day and was continued for 4 weeks. For quantifying TH, DBH, PNMT, NET and VMAT2 mRNA and protein levels we used realtime PCR and Western blot analysis. Results: We observed that CUMS induced increased mRNA levels of catecholamine biosynthetic enzymes (TH, DBH, and PNMT), and noradrenaline transporter NET, while treatment with melatonin decreased these biosynthetic enzymes and transporter. Conversely, CUMS induced a decrease in protein content of TH and NET, while chronic melatonin treatment increased NET protein levels in both control and stressed rats. CUMS and melatonin treatment has no effect on mRNA levels and protein content of VMAT2. Conclusion: This study suggests that the observed decrease of catecholamine biosynthesis and enhanced re-uptake in adrenal medulla of rats exposed to CUMS are connected to the beneficial effects of chronic melatonin treatment.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Melatonin modulates catecholamine biosynthesis and re-uptake in adrenal medulla of rats exposed to chronic unpredictable mild stress",
pages = "77-77",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12289"
}

Stefanović, B., Spasojević, N., Jovanović, P.,& Dronjak, S.. (2017). Melatonin modulates catecholamine biosynthesis and re-uptake in adrenal medulla of rats exposed to chronic unpredictable mild stress. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 77-77.
https://hdl.handle.net/21.15107/rcub_vinar_12289

Stefanović B, Spasojević N, Jovanović P, Dronjak S. Melatonin modulates catecholamine biosynthesis and re-uptake in adrenal medulla of rats exposed to chronic unpredictable mild stress. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:77-77.
https://hdl.handle.net/21.15107/rcub_vinar_12289 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Melatonin modulates catecholamine biosynthesis and re-uptake in adrenal medulla of rats exposed to chronic unpredictable mild stress" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):77-77,
https://hdl.handle.net/21.15107/rcub_vinar_12289 .

TY  - JOUR
AU  - Dronjak, Slađana
AU  - Stefanović, Bojana
AU  - Jovanović, Predrag
AU  - Spasojević, Nataša
AU  - Janković, Milica
AU  - Jeremić, Ivica
AU  - Hoffmann, Markus
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1929
AB  - Baseline sympathetic activity was found to be elevated in rheumatoid arthritis (RA) patients and it is related to increased cardiovascular risk in these patients. Although many studies have highlighted the association between RA and increased cardiac sympathetic activity, the underlying mechanistic links remain unclear. The aim of the present study was to understand how diseases-triggered changes in gene expression may result in maladaptive physiological changes. Our results suggest that the equilibrium between noradrenaline synthesis, release and reuptake was disrupted in the ventricles of arthritic rats. In the acute phase of the arthritic process, decreased gene expression of MAO-A might lead to accumulation of noradrenaline in myocardial interstitial space, whereas increased gene expression of NET protected cardiomyocytes from the deleterious effects of enhanced noradrenaline. During the chronic phase, reduced expression of beta(1)-adrenoceptor and decreased efficiency of noradrenaline reuptake contribute to progressive damage of the myocardium and limits heart efficiency.
T2  - Autonomic Neuroscience: Basic and Clinical
T1  - Altered cardiac gene expression of noradrenaline enzymes, transporter and beta-adrenoceptors in rat model of rheumatoid arthritis
VL  - 208
SP  - 165
EP  - 169
DO  - 10.1016/j.autneu.2017.10.003
ER  - 

@article{
author = "Dronjak, Slađana and Stefanović, Bojana and Jovanović, Predrag and Spasojević, Nataša and Janković, Milica and Jeremić, Ivica and Hoffmann, Markus",
year = "2017",
abstract = "Baseline sympathetic activity was found to be elevated in rheumatoid arthritis (RA) patients and it is related to increased cardiovascular risk in these patients. Although many studies have highlighted the association between RA and increased cardiac sympathetic activity, the underlying mechanistic links remain unclear. The aim of the present study was to understand how diseases-triggered changes in gene expression may result in maladaptive physiological changes. Our results suggest that the equilibrium between noradrenaline synthesis, release and reuptake was disrupted in the ventricles of arthritic rats. In the acute phase of the arthritic process, decreased gene expression of MAO-A might lead to accumulation of noradrenaline in myocardial interstitial space, whereas increased gene expression of NET protected cardiomyocytes from the deleterious effects of enhanced noradrenaline. During the chronic phase, reduced expression of beta(1)-adrenoceptor and decreased efficiency of noradrenaline reuptake contribute to progressive damage of the myocardium and limits heart efficiency.",
journal = "Autonomic Neuroscience: Basic and Clinical",
title = "Altered cardiac gene expression of noradrenaline enzymes, transporter and beta-adrenoceptors in rat model of rheumatoid arthritis",
volume = "208",
pages = "165-169",
doi = "10.1016/j.autneu.2017.10.003"
}

Dronjak, S., Stefanović, B., Jovanović, P., Spasojević, N., Janković, M., Jeremić, I.,& Hoffmann, M.. (2017). Altered cardiac gene expression of noradrenaline enzymes, transporter and beta-adrenoceptors in rat model of rheumatoid arthritis. in Autonomic Neuroscience: Basic and Clinical, 208, 165-169.
https://doi.org/10.1016/j.autneu.2017.10.003

Dronjak S, Stefanović B, Jovanović P, Spasojević N, Janković M, Jeremić I, Hoffmann M. Altered cardiac gene expression of noradrenaline enzymes, transporter and beta-adrenoceptors in rat model of rheumatoid arthritis. in Autonomic Neuroscience: Basic and Clinical. 2017;208:165-169.
doi:10.1016/j.autneu.2017.10.003 .

Dronjak, Slađana, Stefanović, Bojana, Jovanović, Predrag, Spasojević, Nataša, Janković, Milica, Jeremić, Ivica, Hoffmann, Markus, "Altered cardiac gene expression of noradrenaline enzymes, transporter and beta-adrenoceptors in rat model of rheumatoid arthritis" in Autonomic Neuroscience: Basic and Clinical, 208 (2017):165-169,
https://doi.org/10.1016/j.autneu.2017.10.003 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Puškaš, Nela
AU  - Dronjak, Slađana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1074
AB  - Purpose/aim: The adreno-medullar system represents one of the main systems involved in the response to stressful events. The neuropeptide oxytocin, is highly sensitive to the social environment, and regulates autonomic function. Adreno-medullary activity is dependent on the synthesis of catecholamine, its reuptake, release, degradation and vesicular transport. A direct influence of oxytocin on catecholamine synthesizing enzyme and transports in animals exposed to chronic social isolation stress has not been studied yet. Materials and methods: In the present study, we examined the effect of chronic oxytocin treatment on the level of plasma catecholamine and its content, mRNA and protein levels of tyrosine hydroxylase (TH), noradrenaline transporter (NET) as well as vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla of socially isolated rats. Results: Our results show that, by the end of 12 weeks, social isolation did not produce any significant changes in catecholamine content but increased plasma catecholamine level and synthesis in the adrenal medulla. Oxytocin treatment had no further effect either on catecholamine synthesis or content in socially stressed animals whereas a significant elevation of plasma norepinephrine and epinephrine were reduced. On the other hand, chronic isolation caused a significant increase in VMAT2 and decrease in NET protein levels. Oxytocin treatment brought about an increase in protein levels of NET and its return to the levels of control group. Besides, it further increases VMAT2 protein levels in the adrenal medulla of individually housed rats. Conclusion: The present results show that peripheral oxytocin treatment enhances catecholamine uptake and storage in the adrenal medulla of chronically isolated animals.
PB  - Taylor & Francis
T2  - Endocrine Research
T1  - Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress
VL  - 41
IS  - 2
SP  - 124
EP  - 131
DO  - 10.3109/07435800.2015.1094086
ER  - 

@article{
author = "Jovanović, Predrag and Stefanović, Bojana and Spasojević, Nataša and Puškaš, Nela and Dronjak, Slađana",
year = "2016",
abstract = "Purpose/aim: The adreno-medullar system represents one of the main systems involved in the response to stressful events. The neuropeptide oxytocin, is highly sensitive to the social environment, and regulates autonomic function. Adreno-medullary activity is dependent on the synthesis of catecholamine, its reuptake, release, degradation and vesicular transport. A direct influence of oxytocin on catecholamine synthesizing enzyme and transports in animals exposed to chronic social isolation stress has not been studied yet. Materials and methods: In the present study, we examined the effect of chronic oxytocin treatment on the level of plasma catecholamine and its content, mRNA and protein levels of tyrosine hydroxylase (TH), noradrenaline transporter (NET) as well as vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla of socially isolated rats. Results: Our results show that, by the end of 12 weeks, social isolation did not produce any significant changes in catecholamine content but increased plasma catecholamine level and synthesis in the adrenal medulla. Oxytocin treatment had no further effect either on catecholamine synthesis or content in socially stressed animals whereas a significant elevation of plasma norepinephrine and epinephrine were reduced. On the other hand, chronic isolation caused a significant increase in VMAT2 and decrease in NET protein levels. Oxytocin treatment brought about an increase in protein levels of NET and its return to the levels of control group. Besides, it further increases VMAT2 protein levels in the adrenal medulla of individually housed rats. Conclusion: The present results show that peripheral oxytocin treatment enhances catecholamine uptake and storage in the adrenal medulla of chronically isolated animals.",
publisher = "Taylor & Francis",
journal = "Endocrine Research",
title = "Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress",
volume = "41",
number = "2",
pages = "124-131",
doi = "10.3109/07435800.2015.1094086"
}

Jovanović, P., Stefanović, B., Spasojević, N., Puškaš, N.,& Dronjak, S.. (2016). Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress. in Endocrine Research
Taylor & Francis., 41(2), 124-131.
https://doi.org/10.3109/07435800.2015.1094086

Jovanović P, Stefanović B, Spasojević N, Puškaš N, Dronjak S. Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress. in Endocrine Research. 2016;41(2):124-131.
doi:10.3109/07435800.2015.1094086 .

Jovanović, Predrag, Stefanović, Bojana, Spasojević, Nataša, Puškaš, Nela, Dronjak, Slađana, "Effects of oxytocin on adreno-medullary catecholamine synthesis, uptake and storage in rats exposed to chronic isolation stress" in Endocrine Research, 41, no. 2 (2016):124-131,
https://doi.org/10.3109/07435800.2015.1094086 . .

TY  - JOUR
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena D.
AU  - Dronjak, Slađana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1265
AB  - The hippocampus is sensitive to stress which activates norepinephrine terminals deriving from the locus coeruleus. Melatonin exerts positive effects on the hippocampal neurogenic process and on depressive-like behaviour. Thus, in the present study, an examination was made of the effect of chronic melatonin treatment on norepinephrine content, synthesis, uptake, vesicular transport and degradation in the hippocampus of rats exposed to CUMS. This entailed quantifying the norephinephrine, mRNA and protein levels of DBH, NET, VMAT 2, MAO-A and COMT. The results show that CUMS evoked prolonged immobility. Melatonin treatment decreased immobility in comparison with the placebo group, reflecting an antidepressant-like effect. Compared with the placebo group, a dramatic decrease in norepinephrine content, decreased VMAT2 mRNA and protein and increased MAO-A protein levels in the hippocampus of the CUMS rats were observed. However, no significant differences in the levels of DBH, NET, COMT mRNA and protein and MAO-A mRNA levels between the placebo and the stressed groups were found. The results showed the restorative effects of melatonin on the stress-induced decline in the norepinephrine content of the hippocampus. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in VMAT2 mRNA and protein levels, whereas it reduced the increase of the mRNA of COMT and protein levels of MAO-A. Chronic treatment with melatonin failed to alter the gene expression of DBH or NET in the hippocampus of the CUMS rats. Additionally, the results show that melatonin enhances VMAT2 expression and norepinephrine storage, whilst it reduces norepinephrine degrading enzymes. (C) 2016 Elsevier B.V. and ECNP. All rights reserved.
T2  - European Neuropsychopharmacology
T1  - Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels
VL  - 26
IS  - 10
SP  - 1629
EP  - 1637
DO  - 10.1016/j.euroneuro.2016.07.005
ER  - 

@article{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Jasnić, Nebojša and Đorđević, Jelena D. and Dronjak, Slađana",
year = "2016",
abstract = "The hippocampus is sensitive to stress which activates norepinephrine terminals deriving from the locus coeruleus. Melatonin exerts positive effects on the hippocampal neurogenic process and on depressive-like behaviour. Thus, in the present study, an examination was made of the effect of chronic melatonin treatment on norepinephrine content, synthesis, uptake, vesicular transport and degradation in the hippocampus of rats exposed to CUMS. This entailed quantifying the norephinephrine, mRNA and protein levels of DBH, NET, VMAT 2, MAO-A and COMT. The results show that CUMS evoked prolonged immobility. Melatonin treatment decreased immobility in comparison with the placebo group, reflecting an antidepressant-like effect. Compared with the placebo group, a dramatic decrease in norepinephrine content, decreased VMAT2 mRNA and protein and increased MAO-A protein levels in the hippocampus of the CUMS rats were observed. However, no significant differences in the levels of DBH, NET, COMT mRNA and protein and MAO-A mRNA levels between the placebo and the stressed groups were found. The results showed the restorative effects of melatonin on the stress-induced decline in the norepinephrine content of the hippocampus. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in VMAT2 mRNA and protein levels, whereas it reduced the increase of the mRNA of COMT and protein levels of MAO-A. Chronic treatment with melatonin failed to alter the gene expression of DBH or NET in the hippocampus of the CUMS rats. Additionally, the results show that melatonin enhances VMAT2 expression and norepinephrine storage, whilst it reduces norepinephrine degrading enzymes. (C) 2016 Elsevier B.V. and ECNP. All rights reserved.",
journal = "European Neuropsychopharmacology",
title = "Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels",
volume = "26",
number = "10",
pages = "1629-1637",
doi = "10.1016/j.euroneuro.2016.07.005"
}

Stefanović, B., Spasojević, N., Jovanović, P., Jasnić, N., Đorđević, J. D.,& Dronjak, S.. (2016). Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. in European Neuropsychopharmacology, 26(10), 1629-1637.
https://doi.org/10.1016/j.euroneuro.2016.07.005

Stefanović B, Spasojević N, Jovanović P, Jasnić N, Đorđević JD, Dronjak S. Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. in European Neuropsychopharmacology. 2016;26(10):1629-1637.
doi:10.1016/j.euroneuro.2016.07.005 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Jasnić, Nebojša, Đorđević, Jelena D., Dronjak, Slađana, "Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels" in European Neuropsychopharmacology, 26, no. 10 (2016):1629-1637,
https://doi.org/10.1016/j.euroneuro.2016.07.005 . .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1495
AB  - Preclinical studies have shown that melatonin exercised antidepressant-like and anxiolytic-like effects in animal models of anxiety. The aim of the present study was to correlate the changes in behaviour induced by melatonin treatment with the activity of the dopaminergic system in the hippocampus of Wistar rats exposed to chronic, unpredictable, mild stress (CUMS). Male Wistar rats, 11 weeks old, were subjected to chronic stress for 28 successive days. Separate groups of control and stressed rats were intraperitoneally injected daily either with melatonin (10 mg/kg/day, i.p.) or placebo (5% ethanol). The open-field and elevated plus-maze tests were used to assess locomotor activities and anxiety levels. The content of dopamine (DA) in the hippocampal tissues was determined using radioenzymatic assay, while changes in tyrosine hydroxylase (TH) mRNA and protein levels in the hippocampus were determined using real-time RT-PCR and Western immunoblotting. Chronic stress led to reduction in the hippocampal dopaminergic content without affecting the levels of TH protein. These changes were accompanied by increased locomotor activity and higher anxiety levels in the open-field test. Administration of melatonin for 28 days resulted in an increase in the hippocampal DA content as a result of elevated TH protein levels. Melatonin showed an improvement in anxiety-like behaviour along with significantly reduced exploration. We could conclude that melatonin may stimulate dopaminergic synthesis in the hippocampus in order to suppress stress-induced behaviour.
T2  - Folia Biologica
T1  - Anxiety and Hyperlocomotion Induced by Chronic Unpredictable Mild Stress Can Be Moderated with Melatonin Treatment
VL  - 62
IS  - 6
SP  - 250
EP  - 257
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1495
ER  - 

@article{
author = "Spasojević, Nataša and Stefanović, Bojana and Jovanović, Predrag and Dronjak, Slađana",
year = "2016",
abstract = "Preclinical studies have shown that melatonin exercised antidepressant-like and anxiolytic-like effects in animal models of anxiety. The aim of the present study was to correlate the changes in behaviour induced by melatonin treatment with the activity of the dopaminergic system in the hippocampus of Wistar rats exposed to chronic, unpredictable, mild stress (CUMS). Male Wistar rats, 11 weeks old, were subjected to chronic stress for 28 successive days. Separate groups of control and stressed rats were intraperitoneally injected daily either with melatonin (10 mg/kg/day, i.p.) or placebo (5% ethanol). The open-field and elevated plus-maze tests were used to assess locomotor activities and anxiety levels. The content of dopamine (DA) in the hippocampal tissues was determined using radioenzymatic assay, while changes in tyrosine hydroxylase (TH) mRNA and protein levels in the hippocampus were determined using real-time RT-PCR and Western immunoblotting. Chronic stress led to reduction in the hippocampal dopaminergic content without affecting the levels of TH protein. These changes were accompanied by increased locomotor activity and higher anxiety levels in the open-field test. Administration of melatonin for 28 days resulted in an increase in the hippocampal DA content as a result of elevated TH protein levels. Melatonin showed an improvement in anxiety-like behaviour along with significantly reduced exploration. We could conclude that melatonin may stimulate dopaminergic synthesis in the hippocampus in order to suppress stress-induced behaviour.",
journal = "Folia Biologica",
title = "Anxiety and Hyperlocomotion Induced by Chronic Unpredictable Mild Stress Can Be Moderated with Melatonin Treatment",
volume = "62",
number = "6",
pages = "250-257",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1495"
}

Spasojević, N., Stefanović, B., Jovanović, P.,& Dronjak, S.. (2016). Anxiety and Hyperlocomotion Induced by Chronic Unpredictable Mild Stress Can Be Moderated with Melatonin Treatment. in Folia Biologica, 62(6), 250-257.
https://hdl.handle.net/21.15107/rcub_vinar_1495

Spasojević N, Stefanović B, Jovanović P, Dronjak S. Anxiety and Hyperlocomotion Induced by Chronic Unpredictable Mild Stress Can Be Moderated with Melatonin Treatment. in Folia Biologica. 2016;62(6):250-257.
https://hdl.handle.net/21.15107/rcub_vinar_1495 .

Spasojević, Nataša, Stefanović, Bojana, Jovanović, Predrag, Dronjak, Slađana, "Anxiety and Hyperlocomotion Induced by Chronic Unpredictable Mild Stress Can Be Moderated with Melatonin Treatment" in Folia Biologica, 62, no. 6 (2016):250-257,
https://hdl.handle.net/21.15107/rcub_vinar_1495 .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/463
AB  - We have recently shown that chronic fluoxetine treatment acted significantly increasing plasma norepinephrine and epinephrine concentrations both in control and chronically stressed adult male rats. However, possible effects of fluoxetine on catecholamine synthesis and re-uptake in adrenal medulla have been largely unknown. In the present study the effects of chronic fluoxetine treatment on tyrosine hydroxylase, a rate-limiting enzyme in catecholamine synthesis, as well as a norepinephrine transporter and vesicular monoamine transporter 2 gene expressions in adrenal medulla of animals exposed to chronic unpredictable mild stress (CUMS) for 4 weeks, were investigated. Gene expression analyses were performed using a real-time quantitative reverse transcription-PCR. Chronically stressed animals had increased tyrosine hydroxylase mRNA levels and decreased expression of both transporters. Fluoxetine increased tyrosine hydroxylase and decreased norepinephrine transporter gene expression in both unstressed and CUMS rats. These findings suggest that chronic fluoxetine treatment increased plasma catecholamine levels by affecting opposing changes in catecholamine synthesis and uptake.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment
VL  - 87
IS  - 1
SP  - 343
EP  - 350
DO  - 10.1590/0001-3765201520130400
ER  - 

@article{
author = "Spasojević, Nataša and Jovanović, Predrag and Dronjak, Slađana",
year = "2015",
abstract = "We have recently shown that chronic fluoxetine treatment acted significantly increasing plasma norepinephrine and epinephrine concentrations both in control and chronically stressed adult male rats. However, possible effects of fluoxetine on catecholamine synthesis and re-uptake in adrenal medulla have been largely unknown. In the present study the effects of chronic fluoxetine treatment on tyrosine hydroxylase, a rate-limiting enzyme in catecholamine synthesis, as well as a norepinephrine transporter and vesicular monoamine transporter 2 gene expressions in adrenal medulla of animals exposed to chronic unpredictable mild stress (CUMS) for 4 weeks, were investigated. Gene expression analyses were performed using a real-time quantitative reverse transcription-PCR. Chronically stressed animals had increased tyrosine hydroxylase mRNA levels and decreased expression of both transporters. Fluoxetine increased tyrosine hydroxylase and decreased norepinephrine transporter gene expression in both unstressed and CUMS rats. These findings suggest that chronic fluoxetine treatment increased plasma catecholamine levels by affecting opposing changes in catecholamine synthesis and uptake.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment",
volume = "87",
number = "1",
pages = "343-350",
doi = "10.1590/0001-3765201520130400"
}

Spasojević, N., Jovanović, P.,& Dronjak, S.. (2015). Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment. in Anais de Academia Brasileira de Ciencias, 87(1), 343-350.
https://doi.org/10.1590/0001-3765201520130400

Spasojević N, Jovanović P, Dronjak S. Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment. in Anais de Academia Brasileira de Ciencias. 2015;87(1):343-350.
doi:10.1590/0001-3765201520130400 .

Spasojević, Nataša, Jovanović, Predrag, Dronjak, Slađana, "Differential regulation of catecholamine synthesis and transport in rat adrenal medulla by fluoxetine treatment" in Anais de Academia Brasileira de Ciencias, 87, no. 1 (2015):343-350,
https://doi.org/10.1590/0001-3765201520130400 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Božović, N.
AU  - Jasnic, N.
AU  - Đorđević, Jelena D.
AU  - Dronjak, Slađana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5825
AB  - The neuropeptide oxytocin has been shown to influence on neuroendocrine function. The aim of the present study was to investigate the effect of peripheral oxytocin treatment on the synthesis, uptake and content of adreno-medullary catecholamine. For this purpose oxytocin (3.6 mu g/100 g body weight, s.c) was administrated to male rats once a day over 14 days. In order to assess the effect of peripheral oxytocin treatment on adreno-medullary catecholamine we measured epinephrine and norepinephrine content and gene expression of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. Our results show a significant increase of epinephrine (1.7-fold, p LT 0.05) and norepinephrine (1.5-fold, p LT 0.05) content in oxytocin treated animals compared to saline treated ones. Oxytocin treatment had no effect either on mRNA or protein level of TH and NET. Under oxytocin treatment the increase in VMAT2 mRNA level was not statistically significant, but it caused a significant increase in protein level of VMAT2 (3.7-fold, p LT 0.001). These findings indicate that oxytocin treatment increases catecholamine content in the rat adrenal medulla modulating VMAT2 expression. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Peptides
T1  - Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2
VL  - 51
SP  - 110
EP  - 114
DO  - 10.1016/j.peptides.2013.11.001
ER  - 

@article{
author = "Jovanović, Predrag and Spasojević, Nataša and Stefanović, Bojana and Božović, N. and Jasnic, N. and Đorđević, Jelena D. and Dronjak, Slađana",
year = "2014",
abstract = "The neuropeptide oxytocin has been shown to influence on neuroendocrine function. The aim of the present study was to investigate the effect of peripheral oxytocin treatment on the synthesis, uptake and content of adreno-medullary catecholamine. For this purpose oxytocin (3.6 mu g/100 g body weight, s.c) was administrated to male rats once a day over 14 days. In order to assess the effect of peripheral oxytocin treatment on adreno-medullary catecholamine we measured epinephrine and norepinephrine content and gene expression of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. Our results show a significant increase of epinephrine (1.7-fold, p LT 0.05) and norepinephrine (1.5-fold, p LT 0.05) content in oxytocin treated animals compared to saline treated ones. Oxytocin treatment had no effect either on mRNA or protein level of TH and NET. Under oxytocin treatment the increase in VMAT2 mRNA level was not statistically significant, but it caused a significant increase in protein level of VMAT2 (3.7-fold, p LT 0.001). These findings indicate that oxytocin treatment increases catecholamine content in the rat adrenal medulla modulating VMAT2 expression. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Peptides",
title = "Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2",
volume = "51",
pages = "110-114",
doi = "10.1016/j.peptides.2013.11.001"
}

Jovanović, P., Spasojević, N., Stefanović, B., Božović, N., Jasnic, N., Đorđević, J. D.,& Dronjak, S.. (2014). Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2. in Peptides, 51, 110-114.
https://doi.org/10.1016/j.peptides.2013.11.001

Jovanović P, Spasojević N, Stefanović B, Božović N, Jasnic N, Đorđević JD, Dronjak S. Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2. in Peptides. 2014;51:110-114.
doi:10.1016/j.peptides.2013.11.001 .

Jovanović, Predrag, Spasojević, Nataša, Stefanović, Bojana, Božović, N., Jasnic, N., Đorđević, Jelena D., Dronjak, Slađana, "Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2" in Peptides, 51 (2014):110-114,
https://doi.org/10.1016/j.peptides.2013.11.001 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5941
AB  - Chronic isolation stress of adult rat males acted increasing gene expression of tyrosine hydroxylase (TH) and neuronal norepinephrine transporter (NET) in the right stellate ganglia, while vesicular monoamine transporter 2 (VMAT2) level remained unchanged. The stress decreased protein level of TH, as well as mRNA levels for NET and VMAT2 in the left stellate ganglia, but expressed no effect on protein levels of these two transporters. These results demonstrate asymmetry in noradrenegic genes in the right and left stellate ganglia during stress and provide molecular evidence to help explain the difference in response to the stress. (C) 2014 Elsevier B.V. All rights reserved.
T2  - Autonomic Neuroscience: Basic and Clinical
T1  - Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats
VL  - 181
SP  - 85
EP  - 89
DO  - 10.1016/j.autneu.2014.01.002
ER  - 

@article{
author = "Jovanović, Predrag and Spasojević, Nataša and Stefanović, Bojana and Dronjak, Slađana",
year = "2014",
abstract = "Chronic isolation stress of adult rat males acted increasing gene expression of tyrosine hydroxylase (TH) and neuronal norepinephrine transporter (NET) in the right stellate ganglia, while vesicular monoamine transporter 2 (VMAT2) level remained unchanged. The stress decreased protein level of TH, as well as mRNA levels for NET and VMAT2 in the left stellate ganglia, but expressed no effect on protein levels of these two transporters. These results demonstrate asymmetry in noradrenegic genes in the right and left stellate ganglia during stress and provide molecular evidence to help explain the difference in response to the stress. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Autonomic Neuroscience: Basic and Clinical",
title = "Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats",
volume = "181",
pages = "85-89",
doi = "10.1016/j.autneu.2014.01.002"
}

Jovanović, P., Spasojević, N., Stefanović, B.,& Dronjak, S.. (2014). Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats. in Autonomic Neuroscience: Basic and Clinical, 181, 85-89.
https://doi.org/10.1016/j.autneu.2014.01.002

Jovanović P, Spasojević N, Stefanović B, Dronjak S. Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats. in Autonomic Neuroscience: Basic and Clinical. 2014;181:85-89.
doi:10.1016/j.autneu.2014.01.002 .

Jovanović, Predrag, Spasojević, Nataša, Stefanović, Bojana, Dronjak, Slađana, "Differential expression of tyrosine hydroxylase and transporters in the right and left stellate ganglion of socially isolated rats" in Autonomic Neuroscience: Basic and Clinical, 181 (2014):85-89,
https://doi.org/10.1016/j.autneu.2014.01.002 . .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/227
AB  - Social isolation produce various neurochemical alterations in monoamines pathways of the brain. In order to assess the effect of individual housing of adult rats on neurochemical substrates and lateralization in the right and left medial prefrontal cortex (mPFC), we measured dopamine and noradrenaline stores, protein levels of tyrosine-hydroxylase (TH), noradrenaline transporter (NET) and vesicular monoamine transporter 2 (VMAT 2), by Western blot analysis. Individual housing produced no significant changes in dopamine and noradrenaline content, whereas levels of TH and NET were elevated in the right and left mPFC. On the other hand, VMAT 2 protein was reduced in the right mPFC and increased in the left mPFC. The results indicate an increased synthetic and reuptake capacity of noradrenergic terminals in the right and left mPFC and decreased translocation monoamines from the cytoplasm into the intracellular storage vesicles of neurons in the right mPFC.
T2  - Neurochemical Journal
T1  - Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex
VL  - 8
IS  - 4
SP  - 277
EP  - 281
DO  - 10.1134/S1819712414040084
ER  - 

@article{
author = "Spasojević, Nataša and Jovanović, Predrag and Stefanović, Bojana and Dronjak, Slađana",
year = "2014",
abstract = "Social isolation produce various neurochemical alterations in monoamines pathways of the brain. In order to assess the effect of individual housing of adult rats on neurochemical substrates and lateralization in the right and left medial prefrontal cortex (mPFC), we measured dopamine and noradrenaline stores, protein levels of tyrosine-hydroxylase (TH), noradrenaline transporter (NET) and vesicular monoamine transporter 2 (VMAT 2), by Western blot analysis. Individual housing produced no significant changes in dopamine and noradrenaline content, whereas levels of TH and NET were elevated in the right and left mPFC. On the other hand, VMAT 2 protein was reduced in the right mPFC and increased in the left mPFC. The results indicate an increased synthetic and reuptake capacity of noradrenergic terminals in the right and left mPFC and decreased translocation monoamines from the cytoplasm into the intracellular storage vesicles of neurons in the right mPFC.",
journal = "Neurochemical Journal",
title = "Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex",
volume = "8",
number = "4",
pages = "277-281",
doi = "10.1134/S1819712414040084"
}

Spasojević, N., Jovanović, P., Stefanović, B.,& Dronjak, S.. (2014). Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex. in Neurochemical Journal, 8(4), 277-281.
https://doi.org/10.1134/S1819712414040084

Spasojević N, Jovanović P, Stefanović B, Dronjak S. Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex. in Neurochemical Journal. 2014;8(4):277-281.
doi:10.1134/S1819712414040084 .

Spasojević, Nataša, Jovanović, Predrag, Stefanović, Bojana, Dronjak, Slađana, "Influence of individual housing in the adult rats on catecholamine synthesis and storage in the right and left medial prefrontal cortex" in Neurochemical Journal, 8, no. 4 (2014):277-281,
https://doi.org/10.1134/S1819712414040084 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Puškaš, Nela
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Dronjak, Slađana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/300
AB  - Social isolation induced a significant increase in resting heart rate and reduction in heart rate variability. Dysfunction of the intrinsic cardiac nervous system is implicated in the genesis of cardiovascular diseases. Previous evidence suggests that cardiac ganglia contain noradrenergic neurons. Thus, immunohistochemical expression of catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) and vesicular monoamine transporter 2 (VMAT2) were analyzed, as well as the effects of social isolation stress on mRNA and protein levels of this enzyme and transporter in the intrinsic cardiac nervous system of adult rats. Our results indicate that cardiac ganglion neurons express TH and VMAT2 immunoreactivity. Chronic isolated stress of rats caused a decrease in TH mRNA and VMAT2 mRNA in the neurons of intrinsic cardiac ganglia. No significant alterations in the protein levels of TH and VMAT2 were observed in these neurons. These data indicate that the neurons of intrinsic cardiac ganglia express TH as well as VMAT2 but that social isolation stress does not change their protein levels.
T2  - Archives of Biological Sciences
T1  - Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats
VL  - 66
IS  - 4
SP  - 1645
EP  - 1651
DO  - 10.2298/ABS1404645J
ER  - 

@article{
author = "Jovanović, Predrag and Puškaš, Nela and Stefanović, Bojana and Spasojević, Nataša and Dronjak, Slađana",
year = "2014",
abstract = "Social isolation induced a significant increase in resting heart rate and reduction in heart rate variability. Dysfunction of the intrinsic cardiac nervous system is implicated in the genesis of cardiovascular diseases. Previous evidence suggests that cardiac ganglia contain noradrenergic neurons. Thus, immunohistochemical expression of catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) and vesicular monoamine transporter 2 (VMAT2) were analyzed, as well as the effects of social isolation stress on mRNA and protein levels of this enzyme and transporter in the intrinsic cardiac nervous system of adult rats. Our results indicate that cardiac ganglion neurons express TH and VMAT2 immunoreactivity. Chronic isolated stress of rats caused a decrease in TH mRNA and VMAT2 mRNA in the neurons of intrinsic cardiac ganglia. No significant alterations in the protein levels of TH and VMAT2 were observed in these neurons. These data indicate that the neurons of intrinsic cardiac ganglia express TH as well as VMAT2 but that social isolation stress does not change their protein levels.",
journal = "Archives of Biological Sciences",
title = "Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats",
volume = "66",
number = "4",
pages = "1645-1651",
doi = "10.2298/ABS1404645J"
}

Jovanović, P., Puškaš, N., Stefanović, B., Spasojević, N.,& Dronjak, S.. (2014). Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats. in Archives of Biological Sciences, 66(4), 1645-1651.
https://doi.org/10.2298/ABS1404645J

Jovanović P, Puškaš N, Stefanović B, Spasojević N, Dronjak S. Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats. in Archives of Biological Sciences. 2014;66(4):1645-1651.
doi:10.2298/ABS1404645J .

Jovanović, Predrag, Puškaš, Nela, Stefanović, Bojana, Spasojević, Nataša, Dronjak, Slađana, "Immunohistochemical Detection and Gene Expression of Tyrosine Hydroxylase and Vesicular Monoamine Transporter Type 2 in Intrinsic Cardiac Ganglia of Socially Isolated Rats" in Archives of Biological Sciences, 66, no. 4 (2014):1645-1651,
https://doi.org/10.2298/ABS1404645J . .