TY  - JOUR
AU  - Milivojević, Dušan
AU  - Šumonja, Neven
AU  - Medić, Strahinja
AU  - Pavić, Aleksandar B.
AU  - Morić, Ivana
AU  - Vasiljević, Branka
AU  - Senerović, Lidija
AU  - Nikodinović-Runić, Jasmina
PY  - 2018
UR  - https://academic.oup.com/femspd/article/doi/10.1093/femspd/fty041/4978417
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7822
AB  - Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability. © FEMS 2018.
T2  - Pathogens and Disease
T1  - Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans
VL  - 76
IS  - 4
SP  - fty041
DO  - 10.1093/femspd/fty041
ER  - 

@article{
author = "Milivojević, Dušan and Šumonja, Neven and Medić, Strahinja and Pavić, Aleksandar B. and Morić, Ivana and Vasiljević, Branka and Senerović, Lidija and Nikodinović-Runić, Jasmina",
year = "2018",
abstract = "Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability. © FEMS 2018.",
journal = "Pathogens and Disease",
title = "Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans",
volume = "76",
number = "4",
pages = "fty041",
doi = "10.1093/femspd/fty041"
}

Milivojević, D., Šumonja, N., Medić, S., Pavić, A. B., Morić, I., Vasiljević, B., Senerović, L.,& Nikodinović-Runić, J.. (2018). Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. in Pathogens and Disease, 76(4), fty041.
https://doi.org/10.1093/femspd/fty041

Milivojević D, Šumonja N, Medić S, Pavić AB, Morić I, Vasiljević B, Senerović L, Nikodinović-Runić J. Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. in Pathogens and Disease. 2018;76(4):fty041.
doi:10.1093/femspd/fty041 .

Milivojević, Dušan, Šumonja, Neven, Medić, Strahinja, Pavić, Aleksandar B., Morić, Ivana, Vasiljević, Branka, Senerović, Lidija, Nikodinović-Runić, Jasmina, "Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans" in Pathogens and Disease, 76, no. 4 (2018):fty041,
https://doi.org/10.1093/femspd/fty041 . .

TY  - JOUR
AU  - Lazović, Saša
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Senerović, Lidija
AU  - Krivokapić, Nevena
AU  - Mitrović, Tatjana
AU  - Božović, Nikola
AU  - Vasić, Vesna M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1370
AB  - It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((52)-4-methoxy-5-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5H-2,2-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.
T2  - Experimental and Toxicologic Pathology
T1  - Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro
VL  - 69
IS  - 1
SP  - 55
EP  - 62
DO  - 10.1016/j.etp.2016.11.003
ER  - 

@article{
author = "Lazović, Saša and Leskovac, Andreja and Petrović, Sandra and Senerović, Lidija and Krivokapić, Nevena and Mitrović, Tatjana and Božović, Nikola and Vasić, Vesna M. and Nikodinović-Runić, Jasmina",
year = "2017",
abstract = "It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((52)-4-methoxy-5-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5H-2,2-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.",
journal = "Experimental and Toxicologic Pathology",
title = "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro",
volume = "69",
number = "1",
pages = "55-62",
doi = "10.1016/j.etp.2016.11.003"
}

Lazović, S., Leskovac, A., Petrović, S., Senerović, L., Krivokapić, N., Mitrović, T., Božović, N., Vasić, V. M.,& Nikodinović-Runić, J.. (2017). Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology, 69(1), 55-62.
https://doi.org/10.1016/j.etp.2016.11.003

Lazović S, Leskovac A, Petrović S, Senerović L, Krivokapić N, Mitrović T, Božović N, Vasić VM, Nikodinović-Runić J. Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology. 2017;69(1):55-62.
doi:10.1016/j.etp.2016.11.003 .

Lazović, Saša, Leskovac, Andreja, Petrović, Sandra, Senerović, Lidija, Krivokapić, Nevena, Mitrović, Tatjana, Božović, Nikola, Vasić, Vesna M., Nikodinović-Runić, Jasmina, "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro" in Experimental and Toxicologic Pathology, 69, no. 1 (2017):55-62,
https://doi.org/10.1016/j.etp.2016.11.003 . .