TY  - JOUR
AU  - Zurnic, Irena
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Stanković, Aleksandra
AU  - Dinčić, Dragan
AU  - Živković, Maja
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5961
AB  - Background/Aim. Atherosclerosis is still the leading cause of death in Western world. Development of atherosclerotic plaque involves accumulation of inflammatory cells, lipids, smooth muscle cells and extracellular matrix proteins in the intima of the vascular wall. Apolipoprotein E participates in the transport of exogenous cholesterol, endogenously synthesized lipids and triglycerides in the organism. Apolipoprotein E gene has been identified as one of the candidate genes for atherosclerosis. Previous studies in different populations have clearly implicated apolipoprotein E genetic variation (epsilon polymorphisms) as a major modulator of low density lipoprotein cholesterol levels. Data considering apolipoprotein E polymorphisrns in relation to carotid atherosclerosis gave results that are not in full compliance. The aim of present study was to investigate the apolipoprotein E polymorphisms in association with carotid plaque presence, apolipoprotein E and lipid serum levels in patients with carotid atherosclerosis from Serbia. Methods. The study group enrolled 495 participants: 285 controls and 210 consecutive patients with carotid atherosclerosis who underwent carotid endarterectomy. Genotyping of apolipoprotein E polymorphisms were done using polymerase chain reaction and restriction fragment length polymorphism methods. Results. Patients had significantly decreased frequency of the epsilon 2 allele compared to controls. Patients who carry at least one epsilon 2 allele had a significantly higher level of serum apolipoprotein E and significantly lower low density lipoprotein cholesterol levels compared to those who do not carry this allele. Conclusion. Our results suggest protective effect of apolipoprotein E epsilon 2 allele on susceptibility for carotid plaque presence as well as low density lipoprotein cholesterol lowering effect in Serbian patients with carotid atherosclerosis. Further research of multiple gene and environmental factors that contribute to the appearance and the progression of atherosclerosis should be continued with respect to different populations.
T2  - Vojnosanitetski pregled
T1  - Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis
VL  - 71
IS  - 4
SP  - 362
EP  - 367
DO  - 10.2298/VSP1404362Z
ER  - 

@article{
author = "Zurnic, Irena and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra and Dinčić, Dragan and Živković, Maja",
year = "2014",
abstract = "Background/Aim. Atherosclerosis is still the leading cause of death in Western world. Development of atherosclerotic plaque involves accumulation of inflammatory cells, lipids, smooth muscle cells and extracellular matrix proteins in the intima of the vascular wall. Apolipoprotein E participates in the transport of exogenous cholesterol, endogenously synthesized lipids and triglycerides in the organism. Apolipoprotein E gene has been identified as one of the candidate genes for atherosclerosis. Previous studies in different populations have clearly implicated apolipoprotein E genetic variation (epsilon polymorphisms) as a major modulator of low density lipoprotein cholesterol levels. Data considering apolipoprotein E polymorphisrns in relation to carotid atherosclerosis gave results that are not in full compliance. The aim of present study was to investigate the apolipoprotein E polymorphisms in association with carotid plaque presence, apolipoprotein E and lipid serum levels in patients with carotid atherosclerosis from Serbia. Methods. The study group enrolled 495 participants: 285 controls and 210 consecutive patients with carotid atherosclerosis who underwent carotid endarterectomy. Genotyping of apolipoprotein E polymorphisms were done using polymerase chain reaction and restriction fragment length polymorphism methods. Results. Patients had significantly decreased frequency of the epsilon 2 allele compared to controls. Patients who carry at least one epsilon 2 allele had a significantly higher level of serum apolipoprotein E and significantly lower low density lipoprotein cholesterol levels compared to those who do not carry this allele. Conclusion. Our results suggest protective effect of apolipoprotein E epsilon 2 allele on susceptibility for carotid plaque presence as well as low density lipoprotein cholesterol lowering effect in Serbian patients with carotid atherosclerosis. Further research of multiple gene and environmental factors that contribute to the appearance and the progression of atherosclerosis should be continued with respect to different populations.",
journal = "Vojnosanitetski pregled",
title = "Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis",
volume = "71",
number = "4",
pages = "362-367",
doi = "10.2298/VSP1404362Z"
}

Zurnic, I., Đurić, T., Končar, I., Stanković, A., Dinčić, D.,& Živković, M.. (2014). Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis. in Vojnosanitetski pregled, 71(4), 362-367.
https://doi.org/10.2298/VSP1404362Z

Zurnic I, Đurić T, Končar I, Stanković A, Dinčić D, Živković M. Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis. in Vojnosanitetski pregled. 2014;71(4):362-367.
doi:10.2298/VSP1404362Z .

Zurnic, Irena, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, Dinčić, Dragan, Živković, Maja, "Apolipoprotein E gene polymorphisms as risk factors for carotid atherosclerosis" in Vojnosanitetski pregled, 71, no. 4 (2014):362-367,
https://doi.org/10.2298/VSP1404362Z . .

TY  - JOUR
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Radak, Đorđe J.
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Dinčić, Dragan
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4719
AB  - Introduction: The ACE I/D polymorphism was mostly investigated in association with intima-media thickness, rarely with severe atherosclerotic phenotype. Materials and methods: We investigated the association of I/D polymorphism with severe carotid atherosclerosis (CA) (stenosis GT 70%) in asymptomatic and symptomatic patients undergoing carotid endarterectomy. The 504 patients subjected to endarterectomy and 492 healthy controls from a population in Serbia were investigated as a case-control study. Results: The univariate logistic regression analysis revealed ACE DD as a significant risk factor for severe CA (odds ratio [OR] = 1.3, 95% confidence interval [CI] 1.0-1.7, p = 0.04). After adjustment for the common risk factors (age, hypertension, smoking, and HDL) ACE was no longer significant. However, we found a significant independent influence of DD genotype on plaque presence in a normotensive subgroup of patients (OR 1.8, CI 1.2-3.0, p = 0.01, corrected for multiple testing). In symptomatic patients D allele carriers were significantly more frequent compared with asymptomatic patients (OR 1.6 CI 1.0-2.6, p = 0.05). Conclusions: Our data suggests that ACE I/D is not an independent risk factor for severe CA. On the other hand, a significant independent genetic influence of ACE I/D appeared in normotensive and symptomatic patients with severe CA. This should be considered in further research toward resolving the complex genetic background of severe CA phenotype.
T2  - Journal of the Renin-Angiotensin-Aldosterone System
T1  - The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy
VL  - 13
IS  - 1
SP  - 141
EP  - 147
DO  - 10.1177/1470320311423271
ER  - 

@article{
author = "Kolaković, Ana and Živković, Maja and Radak, Đorđe J. and Đurić, Tamara and Končar, Igor and Davidović, Lazar and Dinčić, Dragan and Alavantić, Dragan and Stanković, Aleksandra",
year = "2012",
abstract = "Introduction: The ACE I/D polymorphism was mostly investigated in association with intima-media thickness, rarely with severe atherosclerotic phenotype. Materials and methods: We investigated the association of I/D polymorphism with severe carotid atherosclerosis (CA) (stenosis GT 70%) in asymptomatic and symptomatic patients undergoing carotid endarterectomy. The 504 patients subjected to endarterectomy and 492 healthy controls from a population in Serbia were investigated as a case-control study. Results: The univariate logistic regression analysis revealed ACE DD as a significant risk factor for severe CA (odds ratio [OR] = 1.3, 95% confidence interval [CI] 1.0-1.7, p = 0.04). After adjustment for the common risk factors (age, hypertension, smoking, and HDL) ACE was no longer significant. However, we found a significant independent influence of DD genotype on plaque presence in a normotensive subgroup of patients (OR 1.8, CI 1.2-3.0, p = 0.01, corrected for multiple testing). In symptomatic patients D allele carriers were significantly more frequent compared with asymptomatic patients (OR 1.6 CI 1.0-2.6, p = 0.05). Conclusions: Our data suggests that ACE I/D is not an independent risk factor for severe CA. On the other hand, a significant independent genetic influence of ACE I/D appeared in normotensive and symptomatic patients with severe CA. This should be considered in further research toward resolving the complex genetic background of severe CA phenotype.",
journal = "Journal of the Renin-Angiotensin-Aldosterone System",
title = "The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy",
volume = "13",
number = "1",
pages = "141-147",
doi = "10.1177/1470320311423271"
}

Kolaković, A., Živković, M., Radak, Đ. J., Đurić, T., Končar, I., Davidović, L., Dinčić, D., Alavantić, D.,& Stanković, A.. (2012). The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy. in Journal of the Renin-Angiotensin-Aldosterone System, 13(1), 141-147.
https://doi.org/10.1177/1470320311423271

Kolaković A, Živković M, Radak ĐJ, Đurić T, Končar I, Davidović L, Dinčić D, Alavantić D, Stanković A. The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy. in Journal of the Renin-Angiotensin-Aldosterone System. 2012;13(1):141-147.
doi:10.1177/1470320311423271 .

Kolaković, Ana, Živković, Maja, Radak, Đorđe J., Đurić, Tamara, Končar, Igor, Davidović, Lazar, Dinčić, Dragan, Alavantić, Dragan, Stanković, Aleksandra, "The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy" in Journal of the Renin-Angiotensin-Aldosterone System, 13, no. 1 (2012):141-147,
https://doi.org/10.1177/1470320311423271 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Kolaković, Ana
AU  - Radak, Đorđe J.
AU  - Dinčić, Dragan
AU  - Radak, Sandra
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4823
AB  - Atherosclerosis is known as an inflammatory disease in which a recruitment of leukocytes to the endothelium wall represents a preliminary step of the initiation and the development of disease. The P-selectin glycoprotein ligand (PSGL-1) seems to be the major molecule mediating leukocyte-endothelium interactions and leukocyte rolling on stimulated endothelium. There are limited number of studies reporting on association of Met62Ile SNP in PSGL-1 gene and the risk for atherosclerosis. The aim of this study was to analyze possible association of this polymorphism with an advanced carotid atherosclerosis and biochemical markers of inflammation and haemostasis. The 275 patients consecutively admitted for carotid endarterectomy with stenosis GT 70% and 256 controls of the same ethnic origin were included in the study. The Met62Ile genotypes were determined by PCR RFLP. The Ile/Ile homozygotes had significantly higher CRP compared to the other genotypes in patients. Female patients had Ile allele dose-dependent association with the carotid plaque presence (Met/Met vs. Met/Ile vs. Ile/Ile; OR 1, OR 2.02, CI 1.0-4.08, OR 4.08, CI 1.0-16.81, respectively, p = 0.04). Our results suggest the impact of PSGL-1 Met62Ile polymorphism on inflammation in advanced atherosclerosis. We observed the sex-differential association of Met62Ile with advanced carotid atherosclerosis. Studies in larger and different populations should validate and further examine the suggested role of genetic variations in PSGL-1 with atherosclerosis and thrombosis.
T2  - Molecular Biology Reports
T1  - The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study
VL  - 39
IS  - 6
SP  - 6479
EP  - 6485
DO  - 10.1007/s11033-012-1475-5
ER  - 

@article{
author = "Živković, Maja and Kolaković, Ana and Radak, Đorđe J. and Dinčić, Dragan and Radak, Sandra and Đurić, Tamara and Stanković, Aleksandra",
year = "2012",
abstract = "Atherosclerosis is known as an inflammatory disease in which a recruitment of leukocytes to the endothelium wall represents a preliminary step of the initiation and the development of disease. The P-selectin glycoprotein ligand (PSGL-1) seems to be the major molecule mediating leukocyte-endothelium interactions and leukocyte rolling on stimulated endothelium. There are limited number of studies reporting on association of Met62Ile SNP in PSGL-1 gene and the risk for atherosclerosis. The aim of this study was to analyze possible association of this polymorphism with an advanced carotid atherosclerosis and biochemical markers of inflammation and haemostasis. The 275 patients consecutively admitted for carotid endarterectomy with stenosis GT 70% and 256 controls of the same ethnic origin were included in the study. The Met62Ile genotypes were determined by PCR RFLP. The Ile/Ile homozygotes had significantly higher CRP compared to the other genotypes in patients. Female patients had Ile allele dose-dependent association with the carotid plaque presence (Met/Met vs. Met/Ile vs. Ile/Ile; OR 1, OR 2.02, CI 1.0-4.08, OR 4.08, CI 1.0-16.81, respectively, p = 0.04). Our results suggest the impact of PSGL-1 Met62Ile polymorphism on inflammation in advanced atherosclerosis. We observed the sex-differential association of Met62Ile with advanced carotid atherosclerosis. Studies in larger and different populations should validate and further examine the suggested role of genetic variations in PSGL-1 with atherosclerosis and thrombosis.",
journal = "Molecular Biology Reports",
title = "The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study",
volume = "39",
number = "6",
pages = "6479-6485",
doi = "10.1007/s11033-012-1475-5"
}

Živković, M., Kolaković, A., Radak, Đ. J., Dinčić, D., Radak, S., Đurić, T.,& Stanković, A.. (2012). The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study. in Molecular Biology Reports, 39(6), 6479-6485.
https://doi.org/10.1007/s11033-012-1475-5

Živković M, Kolaković A, Radak ĐJ, Dinčić D, Radak S, Đurić T, Stanković A. The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study. in Molecular Biology Reports. 2012;39(6):6479-6485.
doi:10.1007/s11033-012-1475-5 .

Živković, Maja, Kolaković, Ana, Radak, Đorđe J., Dinčić, Dragan, Radak, Sandra, Đurić, Tamara, Stanković, Aleksandra, "The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study" in Molecular Biology Reports, 39, no. 6 (2012):6479-6485,
https://doi.org/10.1007/s11033-012-1475-5 . .

TY  - JOUR
AU  - Dinčić, Dragan
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
AU  - Vujanic, S
AU  - Gligic, B
AU  - Alavantić, Dragan
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2907
AB  - Objectives: Lipoprotein(a) (Lp(a)) concentration is determined primarily by the apolipoprotein(a) (apo(a)) gene. The pentanucleotide (TTTTA)n repeat and G/A-914 polymorphisms are in the 5 promoter region of the apo(a) gene. To elucidate whether these polymorphisms affect Lp(a) levels, a total of 211 Serbian adults were investigated. esign: One hundred and eleven patients with ischemic heart disease and 100 healthy controls were genotyped and Lp(a) levels determined. Results: Lp(a) concentrations differed according to the (TTTTA)n genotypes: among those having at least one allele 8, patients had significantly higher Lp(a) values than controls. A decreasing trend of Lp(a) values was associated with the -914A allele in controls but the opposite was true in patients. Patients with genotype TTTTA allele 8/AA-914 had significantly higher Lp(a) values than those without allele 8/AA (p LT 0.05). The GT 8 GT 8/GG genotype was not detected. Significant linkage disequilibrium between (TTTTA)n and G/A-914 polymorphism (p LT 0.001) was found. In multivariate regression analysis, the G/A-914 polymorphism significantly (p LT 0.05) affected Lp(a) levels in patients, after taking into account the (TTTTA)n polymorphism. Conclusion: These results indicate that (TTTTA)n and G/A-914 polymorphisms affect Lp(a) levels in ischemic heart disease as a consequence of the linkage disequlibnum.
T2  - Wiener Klinische Wochenschrift
T1  - Apolipoprotein(a) gene polymorphisms (TTTTA)(n) and G/A-914 affect Lp(a) levels in ischemic heart disease patients from Serbia
VL  - 117
IS  - 11-12
SP  - 406
EP  - 411
DO  - 10.1007/s00508-005-0358-3
ER  - 

@article{
author = "Dinčić, Dragan and Živković, Maja and Stanković, Aleksandra and Đurić, Tamara and Vujanic, S and Gligic, B and Alavantić, Dragan",
year = "2005",
abstract = "Objectives: Lipoprotein(a) (Lp(a)) concentration is determined primarily by the apolipoprotein(a) (apo(a)) gene. The pentanucleotide (TTTTA)n repeat and G/A-914 polymorphisms are in the 5 promoter region of the apo(a) gene. To elucidate whether these polymorphisms affect Lp(a) levels, a total of 211 Serbian adults were investigated. esign: One hundred and eleven patients with ischemic heart disease and 100 healthy controls were genotyped and Lp(a) levels determined. Results: Lp(a) concentrations differed according to the (TTTTA)n genotypes: among those having at least one allele 8, patients had significantly higher Lp(a) values than controls. A decreasing trend of Lp(a) values was associated with the -914A allele in controls but the opposite was true in patients. Patients with genotype TTTTA allele 8/AA-914 had significantly higher Lp(a) values than those without allele 8/AA (p LT 0.05). The GT 8 GT 8/GG genotype was not detected. Significant linkage disequilibrium between (TTTTA)n and G/A-914 polymorphism (p LT 0.001) was found. In multivariate regression analysis, the G/A-914 polymorphism significantly (p LT 0.05) affected Lp(a) levels in patients, after taking into account the (TTTTA)n polymorphism. Conclusion: These results indicate that (TTTTA)n and G/A-914 polymorphisms affect Lp(a) levels in ischemic heart disease as a consequence of the linkage disequlibnum.",
journal = "Wiener Klinische Wochenschrift",
title = "Apolipoprotein(a) gene polymorphisms (TTTTA)(n) and G/A-914 affect Lp(a) levels in ischemic heart disease patients from Serbia",
volume = "117",
number = "11-12",
pages = "406-411",
doi = "10.1007/s00508-005-0358-3"
}

Dinčić, D., Živković, M., Stanković, A., Đurić, T., Vujanic, S., Gligic, B.,& Alavantić, D.. (2005). Apolipoprotein(a) gene polymorphisms (TTTTA)(n) and G/A-914 affect Lp(a) levels in ischemic heart disease patients from Serbia. in Wiener Klinische Wochenschrift, 117(11-12), 406-411.
https://doi.org/10.1007/s00508-005-0358-3

Dinčić D, Živković M, Stanković A, Đurić T, Vujanic S, Gligic B, Alavantić D. Apolipoprotein(a) gene polymorphisms (TTTTA)(n) and G/A-914 affect Lp(a) levels in ischemic heart disease patients from Serbia. in Wiener Klinische Wochenschrift. 2005;117(11-12):406-411.
doi:10.1007/s00508-005-0358-3 .

Dinčić, Dragan, Živković, Maja, Stanković, Aleksandra, Đurić, Tamara, Vujanic, S, Gligic, B, Alavantić, Dragan, "Apolipoprotein(a) gene polymorphisms (TTTTA)(n) and G/A-914 affect Lp(a) levels in ischemic heart disease patients from Serbia" in Wiener Klinische Wochenschrift, 117, no. 11-12 (2005):406-411,
https://doi.org/10.1007/s00508-005-0358-3 . .

TY  - CONF
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Dinčić, Dragan
AU  - Stanković, Aleksandra
AU  - Alavantić, Dragan
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6505
C3  - Atherosclerosis Supplements
T1  - Association of MMP-3 5A/6A gene polymorphism with Lp(a) levels in healthy subjects
VL  - 6
IS  - 1
SP  - 95
EP  - 95
DO  - 10.1016/S1567-5688(05)80376-9
ER  - 

@conference{
author = "Živković, Maja and Đurić, Tamara and Dinčić, Dragan and Stanković, Aleksandra and Alavantić, Dragan",
year = "2005",
journal = "Atherosclerosis Supplements",
title = "Association of MMP-3 5A/6A gene polymorphism with Lp(a) levels in healthy subjects",
volume = "6",
number = "1",
pages = "95-95",
doi = "10.1016/S1567-5688(05)80376-9"
}

Živković, M., Đurić, T., Dinčić, D., Stanković, A.,& Alavantić, D.. (2005). Association of MMP-3 5A/6A gene polymorphism with Lp(a) levels in healthy subjects. in Atherosclerosis Supplements, 6(1), 95-95.
https://doi.org/10.1016/S1567-5688(05)80376-9

Živković M, Đurić T, Dinčić D, Stanković A, Alavantić D. Association of MMP-3 5A/6A gene polymorphism with Lp(a) levels in healthy subjects. in Atherosclerosis Supplements. 2005;6(1):95-95.
doi:10.1016/S1567-5688(05)80376-9 .

Živković, Maja, Đurić, Tamara, Dinčić, Dragan, Stanković, Aleksandra, Alavantić, Dragan, "Association of MMP-3 5A/6A gene polymorphism with Lp(a) levels in healthy subjects" in Atherosclerosis Supplements, 6, no. 1 (2005):95-95,
https://doi.org/10.1016/S1567-5688(05)80376-9 . .