TY  - CONF
AU  - Cvetković, S
AU  - Nastasijević, Branislav J.
AU  - Mitić-Ćulafić, D
AU  - Đukanović, S
AU  - Nikolić, B
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10057
AB  - Lipid oxidation that occurs in different types of food can cause alterations in nutritional qualities, flavour, texture and shelf life of foods. Furthermore, high temperature cooking of protein-rich food can lead to formation of heterocyclic aromatic amines capable of compromising the integrity of DNA molecules. To reduce these harmful effects, research has been focused on investigating plants as a source of potential natural food additives and preservatives. Thus, the aim of this study was to estimate antioxidant and antigenotoxic activities of 50% ethanolic-aqueous root and leaf extracts of the medicinal plant, Gentiana lutea . Antioxidative effect was investigated using the DPPH assay, while antigenotoxicity against the mutagen 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine (PhIP) was determined using Salmonella Typhimurium TA 1535 in the SOS/ umuC assay. Leaf extract showed high antioxidative effect with the ability to neutralize up to 87% of free radicals at 400 µg mL -1 . Antigenotoxicity testing revealed that both extracts exhibited remarkable genoprotective activity against PhIP-induced DNA damage, with the highest inhibition levels being 70% and 85% for root and leaf extracts, respectively. Results obtained are encouraging and suggest further research of G. lutea extracts as potential food preservatives and additives in improving food quality and human health.
C3  - IOP Conference Series: Earth and Environmental Science
T1  - Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP
VL  - 854
IS  - 1
SP  - 012018
DO  - 10.1088/1755-1315/854/1/012018
ER  - 

@conference{
author = "Cvetković, S and Nastasijević, Branislav J. and Mitić-Ćulafić, D and Đukanović, S and Nikolić, B",
year = "2021",
abstract = "Lipid oxidation that occurs in different types of food can cause alterations in nutritional qualities, flavour, texture and shelf life of foods. Furthermore, high temperature cooking of protein-rich food can lead to formation of heterocyclic aromatic amines capable of compromising the integrity of DNA molecules. To reduce these harmful effects, research has been focused on investigating plants as a source of potential natural food additives and preservatives. Thus, the aim of this study was to estimate antioxidant and antigenotoxic activities of 50% ethanolic-aqueous root and leaf extracts of the medicinal plant, Gentiana lutea . Antioxidative effect was investigated using the DPPH assay, while antigenotoxicity against the mutagen 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine (PhIP) was determined using Salmonella Typhimurium TA 1535 in the SOS/ umuC assay. Leaf extract showed high antioxidative effect with the ability to neutralize up to 87% of free radicals at 400 µg mL -1 . Antigenotoxicity testing revealed that both extracts exhibited remarkable genoprotective activity against PhIP-induced DNA damage, with the highest inhibition levels being 70% and 85% for root and leaf extracts, respectively. Results obtained are encouraging and suggest further research of G. lutea extracts as potential food preservatives and additives in improving food quality and human health.",
journal = "IOP Conference Series: Earth and Environmental Science",
title = "Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP",
volume = "854",
number = "1",
pages = "012018",
doi = "10.1088/1755-1315/854/1/012018"
}

Cvetković, S., Nastasijević, B. J., Mitić-Ćulafić, D., Đukanović, S.,& Nikolić, B.. (2021). Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP. in IOP Conference Series: Earth and Environmental Science, 854(1), 012018.
https://doi.org/10.1088/1755-1315/854/1/012018

Cvetković S, Nastasijević BJ, Mitić-Ćulafić D, Đukanović S, Nikolić B. Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP. in IOP Conference Series: Earth and Environmental Science. 2021;854(1):012018.
doi:10.1088/1755-1315/854/1/012018 .

Cvetković, S, Nastasijević, Branislav J., Mitić-Ćulafić, D, Đukanović, S, Nikolić, B, "Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP" in IOP Conference Series: Earth and Environmental Science, 854, no. 1 (2021):012018,
https://doi.org/10.1088/1755-1315/854/1/012018 . .

TY  - JOUR
AU  - Maravić-Stojković, V.
AU  - Laušević-Vuk, L.J.
AU  - Jović, M.
AU  - Filipović, M.
AU  - Bojić, Tijana
AU  - Stojković, B.
AU  - Isenović, Esma R.
AU  - Đukanović, B.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8866
AB  - Background: Procalcitonin (PCT) is a marker for sepsis diagnosis, identification of bacterial infection and monitoring of antibiotic therapy. It has been shown that PCT is not a good choice in patients with hemodialysis. Therefore, we have explored two biomarkers: a) presepsin, and, b) midregion-proadrenomedullin (MR-proADM) in patients having End-Stage Renal Disease (ESRD). Patients and Methods: We prospectively enlisted 20 patients, who underwent cardiovascular surgery and had been on dialysis. The diagnosis of sepsis has been established clinically and confirmed by PCT. Blood samples were taken before and after dialysis. Additionally, plasma and sera of 10 healthy blood donors without any complications were used as controls. Results: Presepsin plasma concentrations (4368 ± 3088 vs. 694.1 ± 239.1 pg/mL) were significantly higher in patients with sepsis compared with controls (p<0.0001), but without significance between survivors and nonsurvivors (p=0.77). Circulating levels of MR-proADM (4.15 ± 2.72 vs. 0.294 ± 0.03 nmol/L) were also elevated in ESRD patients (p>0.05) with no significance between alive and deceased (p=0.53) patients. Adjustments have shown that the difference for MR-proADM level is due to the random sampling variability (p=0.989), whereas difference for presepsin remained highly significant (p<0.001). Conclusions: Our results indicate that the accuracy of new biomarkers is equal to that of PCT in patients with ESRD. Presepsin might be as good marker in repeated measurements, before and after dialysis, as it is PCT. © 2014 Maravic-Stojkovic V, et al.
T2  - Journal of Clinical & Experimental Cardiology
T1  - Levels of Presepsin and Midregion-Proadrenomedullin in Septic Patients with End-Stage Renal Disease after Cardiovascular Surgery: 1-Year Follow Up Study
VL  - 5
IS  - 5
DO  - 10.4172/2155-9880.1000307
ER  - 

@article{
author = "Maravić-Stojković, V. and Laušević-Vuk, L.J. and Jović, M. and Filipović, M. and Bojić, Tijana and Stojković, B. and Isenović, Esma R. and Đukanović, B.",
year = "2014",
abstract = "Background: Procalcitonin (PCT) is a marker for sepsis diagnosis, identification of bacterial infection and monitoring of antibiotic therapy. It has been shown that PCT is not a good choice in patients with hemodialysis. Therefore, we have explored two biomarkers: a) presepsin, and, b) midregion-proadrenomedullin (MR-proADM) in patients having End-Stage Renal Disease (ESRD). Patients and Methods: We prospectively enlisted 20 patients, who underwent cardiovascular surgery and had been on dialysis. The diagnosis of sepsis has been established clinically and confirmed by PCT. Blood samples were taken before and after dialysis. Additionally, plasma and sera of 10 healthy blood donors without any complications were used as controls. Results: Presepsin plasma concentrations (4368 ± 3088 vs. 694.1 ± 239.1 pg/mL) were significantly higher in patients with sepsis compared with controls (p<0.0001), but without significance between survivors and nonsurvivors (p=0.77). Circulating levels of MR-proADM (4.15 ± 2.72 vs. 0.294 ± 0.03 nmol/L) were also elevated in ESRD patients (p>0.05) with no significance between alive and deceased (p=0.53) patients. Adjustments have shown that the difference for MR-proADM level is due to the random sampling variability (p=0.989), whereas difference for presepsin remained highly significant (p<0.001). Conclusions: Our results indicate that the accuracy of new biomarkers is equal to that of PCT in patients with ESRD. Presepsin might be as good marker in repeated measurements, before and after dialysis, as it is PCT. © 2014 Maravic-Stojkovic V, et al.",
journal = "Journal of Clinical & Experimental Cardiology",
title = "Levels of Presepsin and Midregion-Proadrenomedullin in Septic Patients with End-Stage Renal Disease after Cardiovascular Surgery: 1-Year Follow Up Study",
volume = "5",
number = "5",
doi = "10.4172/2155-9880.1000307"
}

Maravić-Stojković, V., Laušević-Vuk, L.J., Jović, M., Filipović, M., Bojić, T., Stojković, B., Isenović, E. R.,& Đukanović, B.. (2014). Levels of Presepsin and Midregion-Proadrenomedullin in Septic Patients with End-Stage Renal Disease after Cardiovascular Surgery: 1-Year Follow Up Study. in Journal of Clinical & Experimental Cardiology, 5(5).
https://doi.org/10.4172/2155-9880.1000307

Maravić-Stojković V, Laušević-Vuk L, Jović M, Filipović M, Bojić T, Stojković B, Isenović ER, Đukanović B. Levels of Presepsin and Midregion-Proadrenomedullin in Septic Patients with End-Stage Renal Disease after Cardiovascular Surgery: 1-Year Follow Up Study. in Journal of Clinical & Experimental Cardiology. 2014;5(5).
doi:10.4172/2155-9880.1000307 .

Maravić-Stojković, V., Laušević-Vuk, L.J., Jović, M., Filipović, M., Bojić, Tijana, Stojković, B., Isenović, Esma R., Đukanović, B., "Levels of Presepsin and Midregion-Proadrenomedullin in Septic Patients with End-Stage Renal Disease after Cardiovascular Surgery: 1-Year Follow Up Study" in Journal of Clinical & Experimental Cardiology, 5, no. 5 (2014),
https://doi.org/10.4172/2155-9880.1000307 . .