TY  - JOUR
AU  - Todorović, Ana
AU  - Bobić, Katarina
AU  - Veljković, Filip
AU  - Pejić, Snežana
AU  - Glumac, Sofija
AU  - Stanković, Sanja
AU  - Milovanović, Tijana
AU  - Vukoje, Ivana
AU  - Nedeljković, Jovan
AU  - Radojević Škodrić, Sanja
AU  - Pajović, Snežana B.
AU  - Drakulić, Dunja
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13090
AB  - Nanoparticles (NPs), a distinct class of particles ranging in size from 1 to 100 nm, are one of the most promising technologies of the 21st century, and titanium dioxide NPs (TiO2 NPs) are among the most widely produced and used NPs globally. The increased application of TiO2 NPs raises concerns regarding their global safety and risks of exposure. Many animal studies have reported the accumulation of TiO2 NPs in female reproductive organs; however, evidence of the resultant toxicity remains ambiguous. Since the surface area and chemical modifications of NPs can significantly change their cytotoxicity, we aimed to compare the toxic effects of pristine TiO2 powder with surface-modified TiO2 powders with salicylic acid (TiO2/SA) and 5-aminosalicylic acid (TiO2/5-ASA) on the ovaries, oviducts, and uterus on the 14th day following acute oral treatment. The results, based on alterations in food and water intake, body mass, organ-to-body mass ratio, hormonal status, histological features of tissues of interest, and antioxidant parameters, suggest that the modification with 5-ASA can mitigate some of the observed toxic effects of TiO2 powder and encourage future investigations to create NPs that can potentially reduce the harmful effects of TiO2 NPs while preserving their positive impacts.
T2  - Antioxidants
T1  - Comparable Toxicity of Surface-Modified TiO2 Nanoparticles: An In Vivo Experimental Study on Reproductive Toxicity in Rats
VL  - 13
IS  - 2
SP  - 231
DO  - 10.3390/antiox13020231
ER  - 

@article{
author = "Todorović, Ana and Bobić, Katarina and Veljković, Filip and Pejić, Snežana and Glumac, Sofija and Stanković, Sanja and Milovanović, Tijana and Vukoje, Ivana and Nedeljković, Jovan and Radojević Škodrić, Sanja and Pajović, Snežana B. and Drakulić, Dunja",
year = "2024",
abstract = "Nanoparticles (NPs), a distinct class of particles ranging in size from 1 to 100 nm, are one of the most promising technologies of the 21st century, and titanium dioxide NPs (TiO2 NPs) are among the most widely produced and used NPs globally. The increased application of TiO2 NPs raises concerns regarding their global safety and risks of exposure. Many animal studies have reported the accumulation of TiO2 NPs in female reproductive organs; however, evidence of the resultant toxicity remains ambiguous. Since the surface area and chemical modifications of NPs can significantly change their cytotoxicity, we aimed to compare the toxic effects of pristine TiO2 powder with surface-modified TiO2 powders with salicylic acid (TiO2/SA) and 5-aminosalicylic acid (TiO2/5-ASA) on the ovaries, oviducts, and uterus on the 14th day following acute oral treatment. The results, based on alterations in food and water intake, body mass, organ-to-body mass ratio, hormonal status, histological features of tissues of interest, and antioxidant parameters, suggest that the modification with 5-ASA can mitigate some of the observed toxic effects of TiO2 powder and encourage future investigations to create NPs that can potentially reduce the harmful effects of TiO2 NPs while preserving their positive impacts.",
journal = "Antioxidants",
title = "Comparable Toxicity of Surface-Modified TiO2 Nanoparticles: An In Vivo Experimental Study on Reproductive Toxicity in Rats",
volume = "13",
number = "2",
pages = "231",
doi = "10.3390/antiox13020231"
}

Todorović, A., Bobić, K., Veljković, F., Pejić, S., Glumac, S., Stanković, S., Milovanović, T., Vukoje, I., Nedeljković, J., Radojević Škodrić, S., Pajović, S. B.,& Drakulić, D.. (2024). Comparable Toxicity of Surface-Modified TiO2 Nanoparticles: An In Vivo Experimental Study on Reproductive Toxicity in Rats. in Antioxidants, 13(2), 231.
https://doi.org/10.3390/antiox13020231

Todorović A, Bobić K, Veljković F, Pejić S, Glumac S, Stanković S, Milovanović T, Vukoje I, Nedeljković J, Radojević Škodrić S, Pajović SB, Drakulić D. Comparable Toxicity of Surface-Modified TiO2 Nanoparticles: An In Vivo Experimental Study on Reproductive Toxicity in Rats. in Antioxidants. 2024;13(2):231.
doi:10.3390/antiox13020231 .

Todorović, Ana, Bobić, Katarina, Veljković, Filip, Pejić, Snežana, Glumac, Sofija, Stanković, Sanja, Milovanović, Tijana, Vukoje, Ivana, Nedeljković, Jovan, Radojević Škodrić, Sanja, Pajović, Snežana B., Drakulić, Dunja, "Comparable Toxicity of Surface-Modified TiO2 Nanoparticles: An In Vivo Experimental Study on Reproductive Toxicity in Rats" in Antioxidants, 13, no. 2 (2024):231,
https://doi.org/10.3390/antiox13020231 . .

TY  - JOUR
AU  - Dragićević, Nina
AU  - Predić-Atkinson, Jelena
AU  - Nikolić, Bojan
AU  - Pajović, Snežana B.
AU  - Ivković, Sanja
AU  - Adžić, Miroslav
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12828
AB  - Introduction  Photodynamic therapy (PDT) has gained significant attention due to its superiority over conventional treatments. In the context of skin cancers and nonmalignant skin diseases, topical application of photosensitizer formulations onto affected skin, followed by illumination, offers distinct advantages. Topical PDT simplifies therapy by providing easy access to the skin, increasing drug concentration within the target area, and confining residual photosensitivity to the treated skin. However, the effectiveness of topical PDT is often hindered by challenges such as limited skin penetration or photosensitizer instability. Additionally, the hypoxic tumor environment poses further limitations. Nanocarriers present a promising solution to address these challenges. Areas covered  The objective of this review is to comprehensively explore and highlight the role of various nanocarriers in advancing topical PDT for the treatment of skin diseases. The primary focus is to address the challenges associated with conventional topical PDT approaches and demonstrate how nanotechnology-based strategies can overcome these challenges, thereby improving the overall efficiency and efficacy of PDT. Expert opinion  Nanotechnology has revolutionized the field of PDT, offering innovative tools to combat the unfavorable features of photosensitizers and hurdles in PDT. Nanocarriers enhance skin penetration and stability of photosensitizers, provide controlled drug release, reduce needed dose, increase production of reactive oxygen species, while reducing side effects, thereby improving PDT effectiveness.
T2  - Expert Opinion on Drug Delivery
T1  - Nanocarriers in topical photodynamic therapy
VL  - 21
IS  - 2
DO  - 10.1080/17425247.2024.2318460
ER  - 

@article{
author = "Dragićević, Nina and Predić-Atkinson, Jelena and Nikolić, Bojan and Pajović, Snežana B. and Ivković, Sanja and Adžić, Miroslav",
year = "2024",
abstract = "Introduction  Photodynamic therapy (PDT) has gained significant attention due to its superiority over conventional treatments. In the context of skin cancers and nonmalignant skin diseases, topical application of photosensitizer formulations onto affected skin, followed by illumination, offers distinct advantages. Topical PDT simplifies therapy by providing easy access to the skin, increasing drug concentration within the target area, and confining residual photosensitivity to the treated skin. However, the effectiveness of topical PDT is often hindered by challenges such as limited skin penetration or photosensitizer instability. Additionally, the hypoxic tumor environment poses further limitations. Nanocarriers present a promising solution to address these challenges. Areas covered  The objective of this review is to comprehensively explore and highlight the role of various nanocarriers in advancing topical PDT for the treatment of skin diseases. The primary focus is to address the challenges associated with conventional topical PDT approaches and demonstrate how nanotechnology-based strategies can overcome these challenges, thereby improving the overall efficiency and efficacy of PDT. Expert opinion  Nanotechnology has revolutionized the field of PDT, offering innovative tools to combat the unfavorable features of photosensitizers and hurdles in PDT. Nanocarriers enhance skin penetration and stability of photosensitizers, provide controlled drug release, reduce needed dose, increase production of reactive oxygen species, while reducing side effects, thereby improving PDT effectiveness.",
journal = "Expert Opinion on Drug Delivery",
title = "Nanocarriers in topical photodynamic therapy",
volume = "21",
number = "2",
doi = "10.1080/17425247.2024.2318460"
}

Dragićević, N., Predić-Atkinson, J., Nikolić, B., Pajović, S. B., Ivković, S.,& Adžić, M.. (2024). Nanocarriers in topical photodynamic therapy. in Expert Opinion on Drug Delivery, 21(2).
https://doi.org/10.1080/17425247.2024.2318460

Dragićević N, Predić-Atkinson J, Nikolić B, Pajović SB, Ivković S, Adžić M. Nanocarriers in topical photodynamic therapy. in Expert Opinion on Drug Delivery. 2024;21(2).
doi:10.1080/17425247.2024.2318460 .

Dragićević, Nina, Predić-Atkinson, Jelena, Nikolić, Bojan, Pajović, Snežana B., Ivković, Sanja, Adžić, Miroslav, "Nanocarriers in topical photodynamic therapy" in Expert Opinion on Drug Delivery, 21, no. 2 (2024),
https://doi.org/10.1080/17425247.2024.2318460 . .

TY  - CONF
AU  - Bobić, Katarina
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Veljković, Filip
AU  - Pajović, Snežana
AU  - Nedeljković, Jovan
AU  - Drakulić, Dunja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12810
AB  - Titanium dioxide nanoparticles (Ti02 NPs) are widely used in different cosmetic products, food additives and pharmaceuticals. Their accumulation within the body is associated with adverse health effects. Since animal studies indicate that their outcomes could be ameliorated by different modifications, we tested the impact of Ti02 NPs surface-modified with 5-aminosalicylic acid (Ti02/5-ASA NPs) in female adult Wistar rats. For the purpose of this experiment,14 days following the acute oral intake, the effects of commercially available Ti02 NPs and i02/5-ASA NPs were compared, using well defined methods, to assess the signs of acute toxicity (agitation, convulsion, and mortality) and neurological function (visual placing,
spontaneous activity, and pacing/circling) along with the levels of end products of lipid peroxidation (LPP) in eyes. Our results indicate that following the treatments, spontaneous activity was disturbed while mortality, convulsion and visual placing were unaltered. The modification decreased Ti02 NPs-induced
impairment of agitation and pacing/circling. The insight into biomolecules in the eyes, more precisely into a marker of oxidative stress (LPP), showed that it was decreased after treatment with Ti02/5-ASA NPs in comparison to Ti02 NPs, indicating that this type of modification might mitigate Ti02 NPs-induced oxidative stress. According to obtained results, even though the exact  mechanism of the actions is still unknown, the modification with 5-aminosalicylic acid alleviates toxic effects of commercially available Ti02 NPs.
PB  - Vienna : Young Scientist Association
C3  - 18th YSA PhD Symposium 2023 : Book of abstracts
T1  - Rat toxicity study of orally administrated titanium dioxide nanoparticles modified with 5-aminosalicylic acid
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12810
ER  - 

@conference{
author = "Bobić, Katarina and Todorović, Ana and Pejić, Snežana and Veljković, Filip and Pajović, Snežana and Nedeljković, Jovan and Drakulić, Dunja",
year = "2023",
abstract = "Titanium dioxide nanoparticles (Ti02 NPs) are widely used in different cosmetic products, food additives and pharmaceuticals. Their accumulation within the body is associated with adverse health effects. Since animal studies indicate that their outcomes could be ameliorated by different modifications, we tested the impact of Ti02 NPs surface-modified with 5-aminosalicylic acid (Ti02/5-ASA NPs) in female adult Wistar rats. For the purpose of this experiment,14 days following the acute oral intake, the effects of commercially available Ti02 NPs and i02/5-ASA NPs were compared, using well defined methods, to assess the signs of acute toxicity (agitation, convulsion, and mortality) and neurological function (visual placing,
spontaneous activity, and pacing/circling) along with the levels of end products of lipid peroxidation (LPP) in eyes. Our results indicate that following the treatments, spontaneous activity was disturbed while mortality, convulsion and visual placing were unaltered. The modification decreased Ti02 NPs-induced
impairment of agitation and pacing/circling. The insight into biomolecules in the eyes, more precisely into a marker of oxidative stress (LPP), showed that it was decreased after treatment with Ti02/5-ASA NPs in comparison to Ti02 NPs, indicating that this type of modification might mitigate Ti02 NPs-induced oxidative stress. According to obtained results, even though the exact  mechanism of the actions is still unknown, the modification with 5-aminosalicylic acid alleviates toxic effects of commercially available Ti02 NPs.",
publisher = "Vienna : Young Scientist Association",
journal = "18th YSA PhD Symposium 2023 : Book of abstracts",
title = "Rat toxicity study of orally administrated titanium dioxide nanoparticles modified with 5-aminosalicylic acid",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12810"
}

Bobić, K., Todorović, A., Pejić, S., Veljković, F., Pajović, S., Nedeljković, J.,& Drakulić, D.. (2023). Rat toxicity study of orally administrated titanium dioxide nanoparticles modified with 5-aminosalicylic acid. in 18th YSA PhD Symposium 2023 : Book of abstracts
Vienna : Young Scientist Association..
https://hdl.handle.net/21.15107/rcub_vinar_12810

Bobić K, Todorović A, Pejić S, Veljković F, Pajović S, Nedeljković J, Drakulić D. Rat toxicity study of orally administrated titanium dioxide nanoparticles modified with 5-aminosalicylic acid. in 18th YSA PhD Symposium 2023 : Book of abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_12810 .

Bobić, Katarina, Todorović, Ana, Pejić, Snežana, Veljković, Filip, Pajović, Snežana, Nedeljković, Jovan, Drakulić, Dunja, "Rat toxicity study of orally administrated titanium dioxide nanoparticles modified with 5-aminosalicylic acid" in 18th YSA PhD Symposium 2023 : Book of abstracts (2023),
https://hdl.handle.net/21.15107/rcub_vinar_12810 .

TY  - CONF
AU  - Veljković, Filip
AU  - Drakulić, Dunja
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Bobić, Katarina
AU  - Stajčić, Ivana
AU  - Pajović, Snežana
AU  - Veličković, Suzana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12663
AB  - It is known that the “superalkali” cluster Li2F can significantly improve the hydrogen storage capabilities in carbon materials; boron compounds play a similar role. However, the possibility of interactions of lithium fluoride clusters with boron and hydrogen has not been investigated so far. In this work, a laser desorption/ionization mass spectrometry (LDI MS) was used for this purpose. Preliminary results showed that the interaction between the “superalkali” Li3F cluster, boron and six hydrogen atoms is possible; the ion Li3FBH6+ was detected. Non-stoichiometric clusters of lithium fluoride can also combine with boron and hydrogen in the following manner: Li5F2BH5+ Li5F3BH2+ Li4F5BH4+ Li6F2B6H+, and Li8F6H+.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - Investigation of the possibility of interaction between lithium fluoride clusters and boron using LDI MS
SP  - 176
EP  - 179
DO  - 10.46793/ICCBI23.176V
ER  - 

@conference{
author = "Veljković, Filip and Drakulić, Dunja and Todorović, Ana and Pejić, Snežana and Bobić, Katarina and Stajčić, Ivana and Pajović, Snežana and Veličković, Suzana",
year = "2023",
abstract = "It is known that the “superalkali” cluster Li2F can significantly improve the hydrogen storage capabilities in carbon materials; boron compounds play a similar role. However, the possibility of interactions of lithium fluoride clusters with boron and hydrogen has not been investigated so far. In this work, a laser desorption/ionization mass spectrometry (LDI MS) was used for this purpose. Preliminary results showed that the interaction between the “superalkali” Li3F cluster, boron and six hydrogen atoms is possible; the ion Li3FBH6+ was detected. Non-stoichiometric clusters of lithium fluoride can also combine with boron and hydrogen in the following manner: Li5F2BH5+ Li5F3BH2+ Li4F5BH4+ Li6F2B6H+, and Li8F6H+.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "Investigation of the possibility of interaction between lithium fluoride clusters and boron using LDI MS",
pages = "176-179",
doi = "10.46793/ICCBI23.176V"
}

Veljković, F., Drakulić, D., Todorović, A., Pejić, S., Bobić, K., Stajčić, I., Pajović, S.,& Veličković, S.. (2023). Investigation of the possibility of interaction between lithium fluoride clusters and boron using LDI MS. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 176-179.
https://doi.org/10.46793/ICCBI23.176V

Veljković F, Drakulić D, Todorović A, Pejić S, Bobić K, Stajčić I, Pajović S, Veličković S. Investigation of the possibility of interaction between lithium fluoride clusters and boron using LDI MS. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:176-179.
doi:10.46793/ICCBI23.176V .

Veljković, Filip, Drakulić, Dunja, Todorović, Ana, Pejić, Snežana, Bobić, Katarina, Stajčić, Ivana, Pajović, Snežana, Veličković, Suzana, "Investigation of the possibility of interaction between lithium fluoride clusters and boron using LDI MS" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):176-179,
https://doi.org/10.46793/ICCBI23.176V . .

TY  - CONF
AU  - Đorđević, Neda O.
AU  - Keta, Otilija D.
AU  - Petković, Vladana
AU  - Todorović Vukotić, Nevena
AU  - Stanković, Dalibor
AU  - Tešević, V.
AU  - Pajović, Snežana B.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12594
AB  - This study aimed to determine the phenolic compound content, in vitro antioxidative potential, and cytotoxic effects of four red wine samples: a commercial (V) and three clonal wines (V1, V2, and V3). LC/MS-MS, cyclic voltammetry, and MTT assay techniques were employed for this purpose. Results revealed that all wines were rich in phenolic compounds. Clonal wines outperformed the commercial ones in most phenolic compounds (except myricetin). Notably, V2 and V3 showed the highest levels of gallic acid, catechin, and epicatechin. Among them, V3 exhibited superior antioxidative activity. The MTT assay demonstrated stronger cytotoxic effects of the wine samples on pancreas (Bx-PC3) and colon (HT29) carcinoma cells (47% to 16% and 27% to 7% compared to control, respectively) than on the normal lung fibroblasts (MRC-5) cell line (106% to 77%). It can be concluded that HT29 cells were more sensitive than Bx-PC3 cells. Finally, both clonal and commercial wines serve as valuable sources of polyphenolic compounds, which could have a significant role in preventing cancer and diseases related to oxidative stress.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - In vitro biological effects of clonal red wines
SP  - 180
EP  - 183
DO  - 10.46793/ICCBI23.180DJ
ER  - 

@conference{
author = "Đorđević, Neda O. and Keta, Otilija D. and Petković, Vladana and Todorović Vukotić, Nevena and Stanković, Dalibor and Tešević, V. and Pajović, Snežana B.",
year = "2023",
abstract = "This study aimed to determine the phenolic compound content, in vitro antioxidative potential, and cytotoxic effects of four red wine samples: a commercial (V) and three clonal wines (V1, V2, and V3). LC/MS-MS, cyclic voltammetry, and MTT assay techniques were employed for this purpose. Results revealed that all wines were rich in phenolic compounds. Clonal wines outperformed the commercial ones in most phenolic compounds (except myricetin). Notably, V2 and V3 showed the highest levels of gallic acid, catechin, and epicatechin. Among them, V3 exhibited superior antioxidative activity. The MTT assay demonstrated stronger cytotoxic effects of the wine samples on pancreas (Bx-PC3) and colon (HT29) carcinoma cells (47% to 16% and 27% to 7% compared to control, respectively) than on the normal lung fibroblasts (MRC-5) cell line (106% to 77%). It can be concluded that HT29 cells were more sensitive than Bx-PC3 cells. Finally, both clonal and commercial wines serve as valuable sources of polyphenolic compounds, which could have a significant role in preventing cancer and diseases related to oxidative stress.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "In vitro biological effects of clonal red wines",
pages = "180-183",
doi = "10.46793/ICCBI23.180DJ"
}

Đorđević, N. O., Keta, O. D., Petković, V., Todorović Vukotić, N., Stanković, D., Tešević, V.,& Pajović, S. B.. (2023). In vitro biological effects of clonal red wines. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 180-183.
https://doi.org/10.46793/ICCBI23.180DJ

Đorđević NO, Keta OD, Petković V, Todorović Vukotić N, Stanković D, Tešević V, Pajović SB. In vitro biological effects of clonal red wines. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:180-183.
doi:10.46793/ICCBI23.180DJ .

Đorđević, Neda O., Keta, Otilija D., Petković, Vladana, Todorović Vukotić, Nevena, Stanković, Dalibor, Tešević, V., Pajović, Snežana B., "In vitro biological effects of clonal red wines" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):180-183,
https://doi.org/10.46793/ICCBI23.180DJ . .

TY  - CONF
AU  - Keta, Otilija D.
AU  - Bošković, Jelena
AU  - Petković, Vladana
AU  - Đorđević, Neda O.
AU  - Dobričić, Vladimir
AU  - Čudina, Olivera
AU  - Pajović, Snežana B.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12089
AB  - Among novel cancer chemotherapy approaches, the use of cyclooxygenases (COXs) and lipoxygenases (LOXs) inhibitors represents a promising mean for cancer treatment showing lesser toxicity comparing to the currently used cytotoxic drugs. This study detailed the synthesis of three novel compounds: 1ME, BHTK-AA, and IBU-Ac, each with the capability to concurrently inhibit both COX-2 and 5-LOX. Subsequently, we assessed their effectiveness in inhibiting the proliferation of HeLa cervical and MIA PaCa-2 pancreatic cancer cells. The IC50 values for both examined cell lines were approximately 40 μM, indicating the promising inhibitory potential of the IBU-Ac compound in both types of cancer cells. This finding is positioned to stimulate further investigation into the potential application of IBU-Ac against these particular types of cancers, while also advocating its use in combination with standard anti-cancer protocols, i.e., chemoterapeutics or radiation therapy. The results of this work are also advocating the development and refinement of dual COX-2 and 5-LOX inhibitors, thus improving their efficacy and safety.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines
SP  - 503
EP  - 506
DO  - 10.46793/ICCBI23.503K
ER  - 

@conference{
author = "Keta, Otilija D. and Bošković, Jelena and Petković, Vladana and Đorđević, Neda O. and Dobričić, Vladimir and Čudina, Olivera and Pajović, Snežana B.",
year = "2023",
abstract = "Among novel cancer chemotherapy approaches, the use of cyclooxygenases (COXs) and lipoxygenases (LOXs) inhibitors represents a promising mean for cancer treatment showing lesser toxicity comparing to the currently used cytotoxic drugs. This study detailed the synthesis of three novel compounds: 1ME, BHTK-AA, and IBU-Ac, each with the capability to concurrently inhibit both COX-2 and 5-LOX. Subsequently, we assessed their effectiveness in inhibiting the proliferation of HeLa cervical and MIA PaCa-2 pancreatic cancer cells. The IC50 values for both examined cell lines were approximately 40 μM, indicating the promising inhibitory potential of the IBU-Ac compound in both types of cancer cells. This finding is positioned to stimulate further investigation into the potential application of IBU-Ac against these particular types of cancers, while also advocating its use in combination with standard anti-cancer protocols, i.e., chemoterapeutics or radiation therapy. The results of this work are also advocating the development and refinement of dual COX-2 and 5-LOX inhibitors, thus improving their efficacy and safety.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines",
pages = "503-506",
doi = "10.46793/ICCBI23.503K"
}

Keta, O. D., Bošković, J., Petković, V., Đorđević, N. O., Dobričić, V., Čudina, O.,& Pajović, S. B.. (2023). Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 503-506.
https://doi.org/10.46793/ICCBI23.503K

Keta OD, Bošković J, Petković V, Đorđević NO, Dobričić V, Čudina O, Pajović SB. Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:503-506.
doi:10.46793/ICCBI23.503K .

Keta, Otilija D., Bošković, Jelena, Petković, Vladana, Đorđević, Neda O., Dobričić, Vladimir, Čudina, Olivera, Pajović, Snežana B., "Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):503-506,
https://doi.org/10.46793/ICCBI23.503K . .

TY  - CONF
AU  - Bobić, Katarina
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Veljković, Filip
AU  - Veličković, Suzana
AU  - Pajović, Snežana B.
AU  - Drakulić, Dunja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12389
AB  - Titanium dioxide nanoparticles (TiO2NPs) are widely used in pharmaceutics, food products and cosmetics. Besides numerous beneficial outcomes, they also induce toxic effects in different organs, including the eyes. The recent findings report that some surface modifications can attenuate TiO2NPs toxicity. Thus, the aim of this study was to compare the effects of acute oral treatment with commercially available, bare TiO2NPs and TiO2NPs surface modified with salicylic acid (SA-TiO2NPs) using adult Wistar rats as model organisms. Control animals were gastrically intubated with vehicle (0.01 M HCl), while others were treated with either TiO2NPs or SA-TiO2NPs (1000mg/kg dissolved in vehicle). Afterward, the mortality rate as the acute toxicity parameter and parameters of neurological function were assessed. Animals were sacrificed on the 14th day following treatment. In the eye crude synaptosomal fraction, the effects of treatments on oxidative stress markers such as prooxidant/antioxidant balance (PAB) and lipid peroxidation (LPO) were compared. According to the obtained results, the mortality was 0 in all experimental groups. TiO2NPs and SA-TiO2NPs treatments displayed mild effects on spontaneous activity and pacing and had no impact on the visual placing reflex. Moreover, TiO2NPs and SA-TiO2NPs exposure significantly elevated PAB levels, when compared to controls. Animals treated with SA-TiO2NPs had similar LPO levels as controls, while in TiO2NPs group LPO levels were significantly increased. The presented data showed that both examined nanoparticles exerted similar outcomes referring to most of the investigated parameters, however, surface binding of SA decreased the level of LPO and thus, mitigated some of TiO2NPs toxicity in the eyes.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - TiO2 nanoparticles and TiO2 nanoparticles surface modified with salicylic acid affect neurological functions and oxidative stress markers in the eyes of adult rats
SP  - 499
EP  - 502
DO  - 10.46793/ICCBI23.499B
ER  - 

@conference{
author = "Bobić, Katarina and Todorović, Ana and Pejić, Snežana and Veljković, Filip and Veličković, Suzana and Pajović, Snežana B. and Drakulić, Dunja",
year = "2023",
abstract = "Titanium dioxide nanoparticles (TiO2NPs) are widely used in pharmaceutics, food products and cosmetics. Besides numerous beneficial outcomes, they also induce toxic effects in different organs, including the eyes. The recent findings report that some surface modifications can attenuate TiO2NPs toxicity. Thus, the aim of this study was to compare the effects of acute oral treatment with commercially available, bare TiO2NPs and TiO2NPs surface modified with salicylic acid (SA-TiO2NPs) using adult Wistar rats as model organisms. Control animals were gastrically intubated with vehicle (0.01 M HCl), while others were treated with either TiO2NPs or SA-TiO2NPs (1000mg/kg dissolved in vehicle). Afterward, the mortality rate as the acute toxicity parameter and parameters of neurological function were assessed. Animals were sacrificed on the 14th day following treatment. In the eye crude synaptosomal fraction, the effects of treatments on oxidative stress markers such as prooxidant/antioxidant balance (PAB) and lipid peroxidation (LPO) were compared. According to the obtained results, the mortality was 0 in all experimental groups. TiO2NPs and SA-TiO2NPs treatments displayed mild effects on spontaneous activity and pacing and had no impact on the visual placing reflex. Moreover, TiO2NPs and SA-TiO2NPs exposure significantly elevated PAB levels, when compared to controls. Animals treated with SA-TiO2NPs had similar LPO levels as controls, while in TiO2NPs group LPO levels were significantly increased. The presented data showed that both examined nanoparticles exerted similar outcomes referring to most of the investigated parameters, however, surface binding of SA decreased the level of LPO and thus, mitigated some of TiO2NPs toxicity in the eyes.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "TiO2 nanoparticles and TiO2 nanoparticles surface modified with salicylic acid affect neurological functions and oxidative stress markers in the eyes of adult rats",
pages = "499-502",
doi = "10.46793/ICCBI23.499B"
}

Bobić, K., Todorović, A., Pejić, S., Veljković, F., Veličković, S., Pajović, S. B.,& Drakulić, D.. (2023). TiO2 nanoparticles and TiO2 nanoparticles surface modified with salicylic acid affect neurological functions and oxidative stress markers in the eyes of adult rats. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 499-502.
https://doi.org/10.46793/ICCBI23.499B

Bobić K, Todorović A, Pejić S, Veljković F, Veličković S, Pajović SB, Drakulić D. TiO2 nanoparticles and TiO2 nanoparticles surface modified with salicylic acid affect neurological functions and oxidative stress markers in the eyes of adult rats. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:499-502.
doi:10.46793/ICCBI23.499B .

Bobić, Katarina, Todorović, Ana, Pejić, Snežana, Veljković, Filip, Veličković, Suzana, Pajović, Snežana B., Drakulić, Dunja, "TiO2 nanoparticles and TiO2 nanoparticles surface modified with salicylic acid affect neurological functions and oxidative stress markers in the eyes of adult rats" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):499-502,
https://doi.org/10.46793/ICCBI23.499B . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Stanić, Vojislav
AU  - Jovanović, Dragoljub
AU  - Pejić, Snežana
AU  - Borović, Branka
AU  - Pajović, Snežana B.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12395
AB  - inden honey represents a unique honey variety valued for its nutritional benefits, distinctive taste and aroma. Phenols, polyphenols, flavonoids, ascorbic acid and phenolic acids in honey have antioxidant activities. This study aimed to investigate the effects of linden honey on the activities of antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx), as well as on the concentration of malondialdehyde (MDA) in individually housed animals. The investigated parameters were quantified using spectrophotometric method for determination of enzyme activities and MDA concentration in the blood. We found that treatment with linden honey in the socially isolated animals significantly increased the enzyme activities of CAT and GPx, and significantly decreased the concentration of MDA. The modulation of CAT and GPx activities in socially isolated animals treated with linden honey may be very important for understanding the role of honey in the capacity of antioxidant defense system to increase and maintain its stability in psychosocial stress conditions. Our results may be important in biomedical research for understanding the role of honey in the amelioration of oxidative stress.
AB  - Lipov med predstavlja jedinstvenu vrstu meda cenjenu zbog svoje nutritivne prednosti, specifičnog ukusa i mirisa. Fenoli, polifenoli, fl avonoidi, askorbinska kiselina i fenolne kiseline u medu imaju antioksidativno dejstvo. Ovo istraživanje je imalo za cilj da ispita uticaj lipovog meda na aktivnosti antioksidativnih enzima katalaze (CAT) i glutation peroksidaze (GPx), kao i na koncentraciju malondialdehida (MDA) kod individualno smeštenih životinja. Ispitivani parametri su kvantifi kovani spektrofotometrijskim metodama. Ustanovili smo da tretman lipovim medom, kod socijalno izolovanih životinja, značajno povećava aktivnost enzima CAT i GPx, ali da značajno smanjuje koncentraciju MDA. Modulacija aktivnosti CAT i GPx u krvi socijalno izolovanih životinja tretiranih lipovim medom, može biti veoma važna za razumevanje uloge meda u sposobnosti antioksidativnog odbrambenog sistema da poveća i održi njegovu stabilnost u uslovima psihosocijalnog stresa. Naši rezultati mogu biti važni u biomedicinskim istraživanjima za razumevanje uloge meda u smanjenju oksidativnog stresa.
T2  - Acta Veterinaria
T1  - Antioxidant Status in the Blood of Psychosocially Stressed Rats Treated with Honey
T1  - Antioksidativni status u krvi pacova izlaganih psihosocijalnom stresu tretiranih medom
VL  - 73
IS  - 4
SP  - 464
EP  - 472
DO  - 10.2478/acve-2023-0035
ER  - 

@article{
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Stanić, Vojislav and Jovanović, Dragoljub and Pejić, Snežana and Borović, Branka and Pajović, Snežana B.",
year = "2023",
abstract = "inden honey represents a unique honey variety valued for its nutritional benefits, distinctive taste and aroma. Phenols, polyphenols, flavonoids, ascorbic acid and phenolic acids in honey have antioxidant activities. This study aimed to investigate the effects of linden honey on the activities of antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx), as well as on the concentration of malondialdehyde (MDA) in individually housed animals. The investigated parameters were quantified using spectrophotometric method for determination of enzyme activities and MDA concentration in the blood. We found that treatment with linden honey in the socially isolated animals significantly increased the enzyme activities of CAT and GPx, and significantly decreased the concentration of MDA. The modulation of CAT and GPx activities in socially isolated animals treated with linden honey may be very important for understanding the role of honey in the capacity of antioxidant defense system to increase and maintain its stability in psychosocial stress conditions. Our results may be important in biomedical research for understanding the role of honey in the amelioration of oxidative stress., Lipov med predstavlja jedinstvenu vrstu meda cenjenu zbog svoje nutritivne prednosti, specifičnog ukusa i mirisa. Fenoli, polifenoli, fl avonoidi, askorbinska kiselina i fenolne kiseline u medu imaju antioksidativno dejstvo. Ovo istraživanje je imalo za cilj da ispita uticaj lipovog meda na aktivnosti antioksidativnih enzima katalaze (CAT) i glutation peroksidaze (GPx), kao i na koncentraciju malondialdehida (MDA) kod individualno smeštenih životinja. Ispitivani parametri su kvantifi kovani spektrofotometrijskim metodama. Ustanovili smo da tretman lipovim medom, kod socijalno izolovanih životinja, značajno povećava aktivnost enzima CAT i GPx, ali da značajno smanjuje koncentraciju MDA. Modulacija aktivnosti CAT i GPx u krvi socijalno izolovanih životinja tretiranih lipovim medom, može biti veoma važna za razumevanje uloge meda u sposobnosti antioksidativnog odbrambenog sistema da poveća i održi njegovu stabilnost u uslovima psihosocijalnog stresa. Naši rezultati mogu biti važni u biomedicinskim istraživanjima za razumevanje uloge meda u smanjenju oksidativnog stresa.",
journal = "Acta Veterinaria",
title = "Antioxidant Status in the Blood of Psychosocially Stressed Rats Treated with Honey, Antioksidativni status u krvi pacova izlaganih psihosocijalnom stresu tretiranih medom",
volume = "73",
number = "4",
pages = "464-472",
doi = "10.2478/acve-2023-0035"
}

Gavrilović, L., Stojiljković, V., Stanić, V., Jovanović, D., Pejić, S., Borović, B.,& Pajović, S. B.. (2023). Antioxidant Status in the Blood of Psychosocially Stressed Rats Treated with Honey. in Acta Veterinaria, 73(4), 464-472.
https://doi.org/10.2478/acve-2023-0035

Gavrilović L, Stojiljković V, Stanić V, Jovanović D, Pejić S, Borović B, Pajović SB. Antioxidant Status in the Blood of Psychosocially Stressed Rats Treated with Honey. in Acta Veterinaria. 2023;73(4):464-472.
doi:10.2478/acve-2023-0035 .

Gavrilović, Ljubica, Stojiljković, Vesna, Stanić, Vojislav, Jovanović, Dragoljub, Pejić, Snežana, Borović, Branka, Pajović, Snežana B., "Antioxidant Status in the Blood of Psychosocially Stressed Rats Treated with Honey" in Acta Veterinaria, 73, no. 4 (2023):464-472,
https://doi.org/10.2478/acve-2023-0035 . .

TY  - JOUR
AU  - Đukić, Ivana
AU  - Kaličanin, Nevena
AU  - Senćanski, Milan V.
AU  - Pajović, Snežana B.
AU  - Milićević, Jelena S.
AU  - Prljić, Jelena
AU  - Paessler, Slobodan
AU  - Prodanović, Radivoje
AU  - Glišić, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10629
AB  - Drug resistance is a critical problem in health care that affects therapy outcomes and requires new approaches to drugdesign. SARS-CoV-2 Mpro mutations are of concern as they can potentially reduce therapeutic efficacy. Viral infections are amongst themany disorders for which nutraceuticals have been employed as an adjunct therapy. The aim of this study was to examine the potentialin vitro activity of L-arginine and vitamin C against SARS-CoV-2 Mpro. Methods: The Mpro inhibition assay was developed by cloning,expression, purification, and characterization of Mpro. Selected compounds were then screened for protease inhibition. Results: Largininewas found to be active against SARS-CoV-2 Mpro, while a vitamin C/L-arginine combination had a synergistic antiviral actionagainst Mpro. These findings confirm the results of our previous in silico repurposing study that showed L-arginine and vitamin C werepotential Mpro inhibitors. Moreover, they suggest a possible molecular mechanism to explain the beneficial effect of arginine in COVIDpatients. Conclusions: The findings of the current study are important because they help to identify COVID-19 treatments that areefficient, inexpensive, and have a favorable safety profile. The results of this study also suggest a possible adjuvant nutritional strategyfor COVID-19 that could be used in conjunction with pharmacological agents.
T2  - Frontiers in Bioscience - Landmark
T1  - Inhibition of SARS-CoV-2 Mpro with Vitamin C, L-Arginine and a Vitamin C/L-Arginine Combination
VL  - 28
IS  - 1
SP  - 8
DO  - 10.31083/j.fbl2801008
ER  - 

@article{
author = "Đukić, Ivana and Kaličanin, Nevena and Senćanski, Milan V. and Pajović, Snežana B. and Milićević, Jelena S. and Prljić, Jelena and Paessler, Slobodan and Prodanović, Radivoje and Glišić, Sanja",
year = "2023",
abstract = "Drug resistance is a critical problem in health care that affects therapy outcomes and requires new approaches to drugdesign. SARS-CoV-2 Mpro mutations are of concern as they can potentially reduce therapeutic efficacy. Viral infections are amongst themany disorders for which nutraceuticals have been employed as an adjunct therapy. The aim of this study was to examine the potentialin vitro activity of L-arginine and vitamin C against SARS-CoV-2 Mpro. Methods: The Mpro inhibition assay was developed by cloning,expression, purification, and characterization of Mpro. Selected compounds were then screened for protease inhibition. Results: Largininewas found to be active against SARS-CoV-2 Mpro, while a vitamin C/L-arginine combination had a synergistic antiviral actionagainst Mpro. These findings confirm the results of our previous in silico repurposing study that showed L-arginine and vitamin C werepotential Mpro inhibitors. Moreover, they suggest a possible molecular mechanism to explain the beneficial effect of arginine in COVIDpatients. Conclusions: The findings of the current study are important because they help to identify COVID-19 treatments that areefficient, inexpensive, and have a favorable safety profile. The results of this study also suggest a possible adjuvant nutritional strategyfor COVID-19 that could be used in conjunction with pharmacological agents.",
journal = "Frontiers in Bioscience - Landmark",
title = "Inhibition of SARS-CoV-2 Mpro with Vitamin C, L-Arginine and a Vitamin C/L-Arginine Combination",
volume = "28",
number = "1",
pages = "8",
doi = "10.31083/j.fbl2801008"
}

Đukić, I., Kaličanin, N., Senćanski, M. V., Pajović, S. B., Milićević, J. S., Prljić, J., Paessler, S., Prodanović, R.,& Glišić, S.. (2023). Inhibition of SARS-CoV-2 Mpro with Vitamin C, L-Arginine and a Vitamin C/L-Arginine Combination. in Frontiers in Bioscience - Landmark, 28(1), 8.
https://doi.org/10.31083/j.fbl2801008

Đukić I, Kaličanin N, Senćanski MV, Pajović SB, Milićević JS, Prljić J, Paessler S, Prodanović R, Glišić S. Inhibition of SARS-CoV-2 Mpro with Vitamin C, L-Arginine and a Vitamin C/L-Arginine Combination. in Frontiers in Bioscience - Landmark. 2023;28(1):8.
doi:10.31083/j.fbl2801008 .

Đukić, Ivana, Kaličanin, Nevena, Senćanski, Milan V., Pajović, Snežana B., Milićević, Jelena S., Prljić, Jelena, Paessler, Slobodan, Prodanović, Radivoje, Glišić, Sanja, "Inhibition of SARS-CoV-2 Mpro with Vitamin C, L-Arginine and a Vitamin C/L-Arginine Combination" in Frontiers in Bioscience - Landmark, 28, no. 1 (2023):8,
https://doi.org/10.31083/j.fbl2801008 . .

TY  - CONF
AU  - Bobić, Katarina
AU  - Milovanović, Tijana
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Pejić, Snežana
AU  - Veljković, Filip
AU  - Pajović, Snežana
AU  - Nedeljković, Jovan
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12662
AB  - Široka primena nanočestica titanijum-dioksida (TiO2 NČ) u medicini, farmaceutskoj, prehrambenoj i hemijskoj industriji,1 čini TiO2 NČ visoko zastupljenim u životnoj sredini i svim sferama čovekovog života. Negativne efekte ostvaruju u mozgu, s obzirom da lako prolaze krvno-moždanu barijeru, akumuliraju se u različitim regionima mozga i indukuju oksidativni stres tokom kog se oštećuju svi biomolekuli, uključujući i lipide.2 Literaturni podaci ukazuju da različite modifikacije mogu ublažiti uočene efekte, stoga je cilj ovog rada bio da se uporedi odgovor odraslih Wistar pacova 14 dana nakon jednokratnog oralnog tretmana sa komercijalno dostupnim TiO2 NČ i TiO2 NČ površinski modifikovanim salicilnom (TiO2/SK NČ) odnosno 5- aminosalicilnom kiselinom (TiO2/5-ASK NČ). Odgovarajućim testovima praćene su promene parametara senzorno-motorne funkcije (SMF), kao pokazatelja neurološkog deficita, dok je oksidativni stres praćen promenom nivoa lipidne peroksidacije (LPO) u ukupnom ćelijskom ekstraktu moždanog tkiva. Dobijeni rezultati ukazuju da TiO2/SK NČ ispoljavaju slične efekte kao TiO2 NČ, povećavajući nivo LPO koji može biti uzrok narušene SMF. Sa druge strane, smanjen stepen neurološkog deficita i nivoa oksidativnog stresa nakon TiO2/5- ASK NČ tretmana, mogu se povezati sa povećanjem aktivnosti komponenti antioksidativne zaštite. Stoga, može se pretpostaviti da površinska modifikacija TiO2 NČ sa 5-ASK ima sposobnost ublažavanja štetnih efekata komercijalno dostupnih TiO2 NČ.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka
T1  - Površinska modifikacija nanočestica titanijum dioksida aminosalicilnom kiselinom ublažava neurološki deficit i lipidnu peroksidaciju u mozgu Wistar pacova
SP  - 311
EP  - 311
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12662
ER  - 

@conference{
author = "Bobić, Katarina and Milovanović, Tijana and Todorović, Ana and Drakulić, Dunja and Pejić, Snežana and Veljković, Filip and Pajović, Snežana and Nedeljković, Jovan",
year = "2022",
abstract = "Široka primena nanočestica titanijum-dioksida (TiO2 NČ) u medicini, farmaceutskoj, prehrambenoj i hemijskoj industriji,1 čini TiO2 NČ visoko zastupljenim u životnoj sredini i svim sferama čovekovog života. Negativne efekte ostvaruju u mozgu, s obzirom da lako prolaze krvno-moždanu barijeru, akumuliraju se u različitim regionima mozga i indukuju oksidativni stres tokom kog se oštećuju svi biomolekuli, uključujući i lipide.2 Literaturni podaci ukazuju da različite modifikacije mogu ublažiti uočene efekte, stoga je cilj ovog rada bio da se uporedi odgovor odraslih Wistar pacova 14 dana nakon jednokratnog oralnog tretmana sa komercijalno dostupnim TiO2 NČ i TiO2 NČ površinski modifikovanim salicilnom (TiO2/SK NČ) odnosno 5- aminosalicilnom kiselinom (TiO2/5-ASK NČ). Odgovarajućim testovima praćene su promene parametara senzorno-motorne funkcije (SMF), kao pokazatelja neurološkog deficita, dok je oksidativni stres praćen promenom nivoa lipidne peroksidacije (LPO) u ukupnom ćelijskom ekstraktu moždanog tkiva. Dobijeni rezultati ukazuju da TiO2/SK NČ ispoljavaju slične efekte kao TiO2 NČ, povećavajući nivo LPO koji može biti uzrok narušene SMF. Sa druge strane, smanjen stepen neurološkog deficita i nivoa oksidativnog stresa nakon TiO2/5- ASK NČ tretmana, mogu se povezati sa povećanjem aktivnosti komponenti antioksidativne zaštite. Stoga, može se pretpostaviti da površinska modifikacija TiO2 NČ sa 5-ASK ima sposobnost ublažavanja štetnih efekata komercijalno dostupnih TiO2 NČ.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka",
title = "Površinska modifikacija nanočestica titanijum dioksida aminosalicilnom kiselinom ublažava neurološki deficit i lipidnu peroksidaciju u mozgu Wistar pacova",
pages = "311-311",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12662"
}

Bobić, K., Milovanović, T., Todorović, A., Drakulić, D., Pejić, S., Veljković, F., Pajović, S.,& Nedeljković, J.. (2022). Površinska modifikacija nanočestica titanijum dioksida aminosalicilnom kiselinom ublažava neurološki deficit i lipidnu peroksidaciju u mozgu Wistar pacova. in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka
Beograd : Srpsko biološko društvo., 311-311.
https://hdl.handle.net/21.15107/rcub_vinar_12662

Bobić K, Milovanović T, Todorović A, Drakulić D, Pejić S, Veljković F, Pajović S, Nedeljković J. Površinska modifikacija nanočestica titanijum dioksida aminosalicilnom kiselinom ublažava neurološki deficit i lipidnu peroksidaciju u mozgu Wistar pacova. in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka. 2022;:311-311.
https://hdl.handle.net/21.15107/rcub_vinar_12662 .

Bobić, Katarina, Milovanović, Tijana, Todorović, Ana, Drakulić, Dunja, Pejić, Snežana, Veljković, Filip, Pajović, Snežana, Nedeljković, Jovan, "Površinska modifikacija nanočestica titanijum dioksida aminosalicilnom kiselinom ublažava neurološki deficit i lipidnu peroksidaciju u mozgu Wistar pacova" in Treći kongres biologa Srbije : Osnovna i primenjena istraživanja, metodika nastave : Knjiga sažetaka (2022):311-311,
https://hdl.handle.net/21.15107/rcub_vinar_12662 .

TY  - CONF
AU  - Bobić, Katarina
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Veljković, Filip
AU  - Pajović, Snežana
AU  - Nedeljković, Jovan
AU  - Drakulić, Dunja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12741
AB  - Titanium dioxide nanoparticles (TiO2 NPs) are widely used in many daily necessities, including medicine, food/ beverages, and cosmetics. Besides the numerous benefits of TiO2 NPs utilization, their use raises public concerns since humans are exposed to their toxicity. As different alterations could moderate the observed side effects of commercially available bare TiO2 NPs, we investigated whether TiO2/SA NPs (TiO2 NPs surface‑modified with salicylic acid (SA)) impact the bare TiO2 NPs-induced sensory-motor impairments and eye level of 4-hydroxynonenal (HNE) as the final product of lipid peroxidation and indicator of oxidative stress. Our results demonstrate that 14 days following single oral treatment both investigated TiO2 NPs in adult Wistar rats affected neurological functions and promoted oxidative stress to different extents. Namely, the SA modification alleviated TiO2 NPs-induced asymmetry in four limb movement, while the hind limb clasping reflex and spontaneous activity scores were similar between TiO2 groups. In the eye crude membrane fraction of TiO2/SA NPs-treated rats in comparison to animals treated with bare TiO2 NPs, the HNE level was downregulated. Although SA exhibits the ability to reduce some TiO2 NPs toxicity, due to still unknown mechanism of TiO2 and TiO2/SA NPs actions, further studies are required to verify herein presented findings
PB  - Poland : Nencki Institute of experimental biology
C3  - Acta Neurobiologiae experimentalis : NEURONUS 2022 : 12th Neuronus 2022 Neuroscience Forum : Abstracts
T1  - Surface modification of TiO2 nanoparticles moderates neurological impairments and upregulated oxidative stress indicator in eyes of adult Wistar rats
VL  - 82
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12741
ER  - 

@conference{
author = "Bobić, Katarina and Todorović, Ana and Pejić, Snežana and Veljković, Filip and Pajović, Snežana and Nedeljković, Jovan and Drakulić, Dunja",
year = "2022",
abstract = "Titanium dioxide nanoparticles (TiO2 NPs) are widely used in many daily necessities, including medicine, food/ beverages, and cosmetics. Besides the numerous benefits of TiO2 NPs utilization, their use raises public concerns since humans are exposed to their toxicity. As different alterations could moderate the observed side effects of commercially available bare TiO2 NPs, we investigated whether TiO2/SA NPs (TiO2 NPs surface‑modified with salicylic acid (SA)) impact the bare TiO2 NPs-induced sensory-motor impairments and eye level of 4-hydroxynonenal (HNE) as the final product of lipid peroxidation and indicator of oxidative stress. Our results demonstrate that 14 days following single oral treatment both investigated TiO2 NPs in adult Wistar rats affected neurological functions and promoted oxidative stress to different extents. Namely, the SA modification alleviated TiO2 NPs-induced asymmetry in four limb movement, while the hind limb clasping reflex and spontaneous activity scores were similar between TiO2 groups. In the eye crude membrane fraction of TiO2/SA NPs-treated rats in comparison to animals treated with bare TiO2 NPs, the HNE level was downregulated. Although SA exhibits the ability to reduce some TiO2 NPs toxicity, due to still unknown mechanism of TiO2 and TiO2/SA NPs actions, further studies are required to verify herein presented findings",
publisher = "Poland : Nencki Institute of experimental biology",
journal = "Acta Neurobiologiae experimentalis : NEURONUS 2022 : 12th Neuronus 2022 Neuroscience Forum : Abstracts",
title = "Surface modification of TiO2 nanoparticles moderates neurological impairments and upregulated oxidative stress indicator in eyes of adult Wistar rats",
volume = "82",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12741"
}

Bobić, K., Todorović, A., Pejić, S., Veljković, F., Pajović, S., Nedeljković, J.,& Drakulić, D.. (2022). Surface modification of TiO2 nanoparticles moderates neurological impairments and upregulated oxidative stress indicator in eyes of adult Wistar rats. in Acta Neurobiologiae experimentalis : NEURONUS 2022 : 12th Neuronus 2022 Neuroscience Forum : Abstracts
Poland : Nencki Institute of experimental biology., 82.
https://hdl.handle.net/21.15107/rcub_vinar_12741

Bobić K, Todorović A, Pejić S, Veljković F, Pajović S, Nedeljković J, Drakulić D. Surface modification of TiO2 nanoparticles moderates neurological impairments and upregulated oxidative stress indicator in eyes of adult Wistar rats. in Acta Neurobiologiae experimentalis : NEURONUS 2022 : 12th Neuronus 2022 Neuroscience Forum : Abstracts. 2022;82.
https://hdl.handle.net/21.15107/rcub_vinar_12741 .

Bobić, Katarina, Todorović, Ana, Pejić, Snežana, Veljković, Filip, Pajović, Snežana, Nedeljković, Jovan, Drakulić, Dunja, "Surface modification of TiO2 nanoparticles moderates neurological impairments and upregulated oxidative stress indicator in eyes of adult Wistar rats" in Acta Neurobiologiae experimentalis : NEURONUS 2022 : 12th Neuronus 2022 Neuroscience Forum : Abstracts, 82 (2022),
https://hdl.handle.net/21.15107/rcub_vinar_12741 .

TY  - JOUR
AU  - Đorđević, Neda O.
AU  - Stanisavljević, Nemanja
AU  - Todorović Vukotić, Nevena
AU  - Novović, Katarina
AU  - Žakula, Jelena
AU  - Stanković, Dalibor M.
AU  - Pajović, Snežana B.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10353
AB  - The beneficial effect of moderate wine consumption is attributed to its micronutrients, especially polyphenols and largely depends on the digestion process. This work aimed to examine the influence of in vitro simulated digestion in the presence of complex food matrix on antioxidant and cytotoxic activity of red wine. The obtained results showed that total phenolic content of wine sample after in vitro digestion was higher compared to undigested wine, while the antioxidant activity of these samples was similar before and after digestion. Furthermore, it has been noticed that digested wine showed cytotoxic activity on SKBR3 breast adenocarcinoma cells near 20% after 72 h of treatment. This pioneering study that examined biological potential of in vitro digested wine in the presence of complex food matrix indicate that antioxidant and cytotoxic activity of red wine is preserved after digestion.
T2  - Natural Product Research
T1  - Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix
SP  - 1
EP  - 6
DO  - 10.1080/14786419.2022.2095380
ER  - 

@article{
author = "Đorđević, Neda O. and Stanisavljević, Nemanja and Todorović Vukotić, Nevena and Novović, Katarina and Žakula, Jelena and Stanković, Dalibor M. and Pajović, Snežana B.",
year = "2022",
abstract = "The beneficial effect of moderate wine consumption is attributed to its micronutrients, especially polyphenols and largely depends on the digestion process. This work aimed to examine the influence of in vitro simulated digestion in the presence of complex food matrix on antioxidant and cytotoxic activity of red wine. The obtained results showed that total phenolic content of wine sample after in vitro digestion was higher compared to undigested wine, while the antioxidant activity of these samples was similar before and after digestion. Furthermore, it has been noticed that digested wine showed cytotoxic activity on SKBR3 breast adenocarcinoma cells near 20% after 72 h of treatment. This pioneering study that examined biological potential of in vitro digested wine in the presence of complex food matrix indicate that antioxidant and cytotoxic activity of red wine is preserved after digestion.",
journal = "Natural Product Research",
title = "Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix",
pages = "1-6",
doi = "10.1080/14786419.2022.2095380"
}

Đorđević, N. O., Stanisavljević, N., Todorović Vukotić, N., Novović, K., Žakula, J., Stanković, D. M.,& Pajović, S. B.. (2022). Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix. in Natural Product Research, 1-6.
https://doi.org/10.1080/14786419.2022.2095380

Đorđević NO, Stanisavljević N, Todorović Vukotić N, Novović K, Žakula J, Stanković DM, Pajović SB. Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix. in Natural Product Research. 2022;:1-6.
doi:10.1080/14786419.2022.2095380 .

Đorđević, Neda O., Stanisavljević, Nemanja, Todorović Vukotić, Nevena, Novović, Katarina, Žakula, Jelena, Stanković, Dalibor M., Pajović, Snežana B., "Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix" in Natural Product Research (2022):1-6,
https://doi.org/10.1080/14786419.2022.2095380 . .

TY  - CONF
AU  - Đorđević, Neda
AU  - Todorović Vukotić, Nevena
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Pejić, Snežana
AU  - Pajović, Snežana B.
AU  - Tešević, V.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12586
AB  - Positive effects of moderate wine consumption in individuals with cardiovascular disease, hypertension, diabetes, and cancers have been shown in numerous epidemiological and clinical studies. This research examined the phenolic content of commercial and two clonal Merlot wines as well as their biological potential. The obtained results indicated that all analyzed samples were a good source of phenolic compounds. Cytotoxicity assay on melanoma (A375) and cervical (HeLa) cancer cell lines have shown that all analyzed wines inhibited the growth of human cancer cells in vitro with differing susceptibility among tested cell lines. Clonal wines in the volume ratio of 10 and 20% showed to be more efficient anti-proliferative agents than commercial wine regarding the A375 cells. This could be connected with higher total phenolic content in clonal wines. The effect of all analyzed samples on the A375 cells was greater compared to HeLa cell line.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2022 : Book of Abstracts
T1  - Phenolic Composition and Cytotoxic Activity of Red Wine
SP  - 153
EP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12586
ER  - 

@conference{
author = "Đorđević, Neda and Todorović Vukotić, Nevena and Korićanac, Lela and Žakula, Jelena and Pejić, Snežana and Pajović, Snežana B. and Tešević, V.",
year = "2022",
abstract = "Positive effects of moderate wine consumption in individuals with cardiovascular disease, hypertension, diabetes, and cancers have been shown in numerous epidemiological and clinical studies. This research examined the phenolic content of commercial and two clonal Merlot wines as well as their biological potential. The obtained results indicated that all analyzed samples were a good source of phenolic compounds. Cytotoxicity assay on melanoma (A375) and cervical (HeLa) cancer cell lines have shown that all analyzed wines inhibited the growth of human cancer cells in vitro with differing susceptibility among tested cell lines. Clonal wines in the volume ratio of 10 and 20% showed to be more efficient anti-proliferative agents than commercial wine regarding the A375 cells. This could be connected with higher total phenolic content in clonal wines. The effect of all analyzed samples on the A375 cells was greater compared to HeLa cell line.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2022 : Book of Abstracts",
title = "Phenolic Composition and Cytotoxic Activity of Red Wine",
pages = "153-153",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12586"
}

Đorđević, N., Todorović Vukotić, N., Korićanac, L., Žakula, J., Pejić, S., Pajović, S. B.,& Tešević, V.. (2022). Phenolic Composition and Cytotoxic Activity of Red Wine. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2022 : Book of Abstracts
Belgrade : Society of Physical Chemists of Serbia., 153-153.
https://hdl.handle.net/21.15107/rcub_vinar_12586

Đorđević N, Todorović Vukotić N, Korićanac L, Žakula J, Pejić S, Pajović SB, Tešević V. Phenolic Composition and Cytotoxic Activity of Red Wine. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2022 : Book of Abstracts. 2022;:153-153.
https://hdl.handle.net/21.15107/rcub_vinar_12586 .

Đorđević, Neda, Todorović Vukotić, Nevena, Korićanac, Lela, Žakula, Jelena, Pejić, Snežana, Pajović, Snežana B., Tešević, V., "Phenolic Composition and Cytotoxic Activity of Red Wine" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2022 : Book of Abstracts (2022):153-153,
https://hdl.handle.net/21.15107/rcub_vinar_12586 .

TY  - CONF
AU  - Đorđević, Neda
AU  - Todorović Vukotić, Nevena
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Pejić, Snežana
AU  - Tešević, V.
AU  - Pajović, Snežana B.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12592
AB  - Benefitni efekat crvenog vina na zdravlje ljudi od davnina je poznat. Među alkoholnim pićima crveno vino pokazuje najjači efekat protiv pojave oboljenja povezanih sa oksidativnim stresom, kao što su kardiovaskularna, neurodegenerativna oboljenja, dijabetes i kancer.1 U okviru ove studije ispitan je citotoksični efekat crvenog vina sorte merlo (komercijalno dostupno vino i vina dobijena od klonova VCR1 i VCR101) poreklom iz Crne Gore, berba 2011, u odnosu na ćelijsku liniju kancera pankreasa (PANC-1) i ćelijsku liniju melanoma (A375). Citotoksični efekat vina je određen SRB metodom kroz praćenje ćelijskog preživljavanja kancerskih ćelija 48 sati nakon tretmana sa tri različita zapreminska procenta analiziranih vina (5, 10 i 20%). Dobijeni rezultati su pokazali citotoksični efekat svih analiziranih vina na obe ćelijske linije. Ćelijsko preživljavanje nakon tretmana vinima se kretalo od 36 do 64%. Citotoksični efekat svih vina u odnosu na A375 ćelije je bio veći nego na PANC-1 ćelijsku liniju, što je od posebnog značaja ako se uzme u obzir rezistentnost melanomskih ćelija na postojeće terapeutske tretmane. Takođe, klonska sortna vina su pokazala veći citotoksični efekat na A375 ćelijsku liniju od komercijalno dostupnog vina. Dobijeni rezultati su ukazali da umereno konzumiranje crvenog vina sorte merlo sa specifičnim geografskim poreklom predstavlja dobar izvor bioaktivnih komponenata sa pozitivnim biološkim efektom, što je od posebne važnosti za klonska sortna vina.
PB  - Beograd : Srpsko biološko društvo
C3  - 3. kongres biologa Srbije : osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka
T1  - Citotoksično dejstvo crvenog vina na tumorske ćelije u in vitro uslovima
SP  - 321
EP  - 321
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12592
ER  - 

@conference{
author = "Đorđević, Neda and Todorović Vukotić, Nevena and Korićanac, Lela and Žakula, Jelena and Pejić, Snežana and Tešević, V. and Pajović, Snežana B.",
year = "2022",
abstract = "Benefitni efekat crvenog vina na zdravlje ljudi od davnina je poznat. Među alkoholnim pićima crveno vino pokazuje najjači efekat protiv pojave oboljenja povezanih sa oksidativnim stresom, kao što su kardiovaskularna, neurodegenerativna oboljenja, dijabetes i kancer.1 U okviru ove studije ispitan je citotoksični efekat crvenog vina sorte merlo (komercijalno dostupno vino i vina dobijena od klonova VCR1 i VCR101) poreklom iz Crne Gore, berba 2011, u odnosu na ćelijsku liniju kancera pankreasa (PANC-1) i ćelijsku liniju melanoma (A375). Citotoksični efekat vina je određen SRB metodom kroz praćenje ćelijskog preživljavanja kancerskih ćelija 48 sati nakon tretmana sa tri različita zapreminska procenta analiziranih vina (5, 10 i 20%). Dobijeni rezultati su pokazali citotoksični efekat svih analiziranih vina na obe ćelijske linije. Ćelijsko preživljavanje nakon tretmana vinima se kretalo od 36 do 64%. Citotoksični efekat svih vina u odnosu na A375 ćelije je bio veći nego na PANC-1 ćelijsku liniju, što je od posebnog značaja ako se uzme u obzir rezistentnost melanomskih ćelija na postojeće terapeutske tretmane. Takođe, klonska sortna vina su pokazala veći citotoksični efekat na A375 ćelijsku liniju od komercijalno dostupnog vina. Dobijeni rezultati su ukazali da umereno konzumiranje crvenog vina sorte merlo sa specifičnim geografskim poreklom predstavlja dobar izvor bioaktivnih komponenata sa pozitivnim biološkim efektom, što je od posebne važnosti za klonska sortna vina.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "3. kongres biologa Srbije : osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka",
title = "Citotoksično dejstvo crvenog vina na tumorske ćelije u in vitro uslovima",
pages = "321-321",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12592"
}

Đorđević, N., Todorović Vukotić, N., Korićanac, L., Žakula, J., Pejić, S., Tešević, V.,& Pajović, S. B.. (2022). Citotoksično dejstvo crvenog vina na tumorske ćelije u in vitro uslovima. in 3. kongres biologa Srbije : osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka
Beograd : Srpsko biološko društvo., 321-321.
https://hdl.handle.net/21.15107/rcub_vinar_12592

Đorđević N, Todorović Vukotić N, Korićanac L, Žakula J, Pejić S, Tešević V, Pajović SB. Citotoksično dejstvo crvenog vina na tumorske ćelije u in vitro uslovima. in 3. kongres biologa Srbije : osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka. 2022;:321-321.
https://hdl.handle.net/21.15107/rcub_vinar_12592 .

Đorđević, Neda, Todorović Vukotić, Nevena, Korićanac, Lela, Žakula, Jelena, Pejić, Snežana, Tešević, V., Pajović, Snežana B., "Citotoksično dejstvo crvenog vina na tumorske ćelije u in vitro uslovima" in 3. kongres biologa Srbije : osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka (2022):321-321,
https://hdl.handle.net/21.15107/rcub_vinar_12592 .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Vujović, Predrag
AU  - Pajović, Snežana B.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10235
AB  - Background This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS). Methods The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities. Results We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals. Conclusions These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understanding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress.
T2  - PeerJ
T1  - Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium
VL  - 10
SP  - e13020
DO  - 10.7717/peerj.13020
ER  - 

@article{
author = "Gavrilović, Ljubica and Popović, Nataša and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Vujović, Predrag and Pajović, Snežana B.",
year = "2022",
abstract = "Background This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS). Methods The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities. Results We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals. Conclusions These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understanding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress.",
journal = "PeerJ",
title = "Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium",
volume = "10",
pages = "e13020",
doi = "10.7717/peerj.13020"
}

Gavrilović, L., Popović, N., Stojiljković, V., Pejić, S., Todorović, A., Vujović, P.,& Pajović, S. B.. (2022). Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium. in PeerJ, 10, e13020.
https://doi.org/10.7717/peerj.13020

Gavrilović L, Popović N, Stojiljković V, Pejić S, Todorović A, Vujović P, Pajović SB. Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium. in PeerJ. 2022;10:e13020.
doi:10.7717/peerj.13020 .

Gavrilović, Ljubica, Popović, Nataša, Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Vujović, Predrag, Pajović, Snežana B., "Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium" in PeerJ, 10 (2022):e13020,
https://doi.org/10.7717/peerj.13020 . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Spasojević-Tišma, Vera D.
AU  - Nikolić, Dragan
AU  - Pajović, Snežana B.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10374
AB  - The aim of this study was to examine whether there are differences in adrenomedullary function in respect to the left and right sides in chronic stress conditions. We investigated how chronic stress isolation (CSI 12 weeks) affected the protein levels of key enzymes involved in adrenaline (A) synthesis (phenyl ethanolamine N-methyltransferase -PNMT), storage (vesicular monoamine transporters 2 - VMAT2) and degradation (catechol-O-methyltransferase - COMT), as well as the concentrations of A as an index for adrenomedullary function in the left and right adrenal medulla. Also, we examined the concentrations of malondialdehyde (MDA), protein levels of nuclear factor κB (NF-κB), and activity of catalase (CAT) in the left and right adrenal medulla. The investigated parameters were quantified by Western blot analysis, assay of enzymatic activity, and CAT Research ELISA kits. We found that CSI pro duced significantly increased levels of PNMT protein, and VMAT2 protein, as well as increased concentrations of A in the right adrenal medulla. However, we recorded that CSI increased protein levels of COMT and NF-κB, as well as the concentrations of MDA in the left adrenal medulla. Also, CSI decreased the activity of CAT only in the left adrenal medulla. Based on these results, it may be
T2  - Acta Veterinaria
T1  - Differences in the Functional Activity and Redox Homeostasis Between the Left and Right Adrenal Gland of Rats Exposed to Chronic Isolation Stress
VL  - 72
IS  - 2
SP  - 224
EP  - 234
DO  - 10.2478/acve-2022-0018
ER  - 

@article{
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Pejić, Snežana and Spasojević-Tišma, Vera D. and Nikolić, Dragan and Pajović, Snežana B.",
year = "2022",
abstract = "The aim of this study was to examine whether there are differences in adrenomedullary function in respect to the left and right sides in chronic stress conditions. We investigated how chronic stress isolation (CSI 12 weeks) affected the protein levels of key enzymes involved in adrenaline (A) synthesis (phenyl ethanolamine N-methyltransferase -PNMT), storage (vesicular monoamine transporters 2 - VMAT2) and degradation (catechol-O-methyltransferase - COMT), as well as the concentrations of A as an index for adrenomedullary function in the left and right adrenal medulla. Also, we examined the concentrations of malondialdehyde (MDA), protein levels of nuclear factor κB (NF-κB), and activity of catalase (CAT) in the left and right adrenal medulla. The investigated parameters were quantified by Western blot analysis, assay of enzymatic activity, and CAT Research ELISA kits. We found that CSI pro duced significantly increased levels of PNMT protein, and VMAT2 protein, as well as increased concentrations of A in the right adrenal medulla. However, we recorded that CSI increased protein levels of COMT and NF-κB, as well as the concentrations of MDA in the left adrenal medulla. Also, CSI decreased the activity of CAT only in the left adrenal medulla. Based on these results, it may be",
journal = "Acta Veterinaria",
title = "Differences in the Functional Activity and Redox Homeostasis Between the Left and Right Adrenal Gland of Rats Exposed to Chronic Isolation Stress",
volume = "72",
number = "2",
pages = "224-234",
doi = "10.2478/acve-2022-0018"
}

Gavrilović, L., Stojiljković, V., Pejić, S., Spasojević-Tišma, V. D., Nikolić, D.,& Pajović, S. B.. (2022). Differences in the Functional Activity and Redox Homeostasis Between the Left and Right Adrenal Gland of Rats Exposed to Chronic Isolation Stress. in Acta Veterinaria, 72(2), 224-234.
https://doi.org/10.2478/acve-2022-0018

Gavrilović L, Stojiljković V, Pejić S, Spasojević-Tišma VD, Nikolić D, Pajović SB. Differences in the Functional Activity and Redox Homeostasis Between the Left and Right Adrenal Gland of Rats Exposed to Chronic Isolation Stress. in Acta Veterinaria. 2022;72(2):224-234.
doi:10.2478/acve-2022-0018 .

Gavrilović, Ljubica, Stojiljković, Vesna, Pejić, Snežana, Spasojević-Tišma, Vera D., Nikolić, Dragan, Pajović, Snežana B., "Differences in the Functional Activity and Redox Homeostasis Between the Left and Right Adrenal Gland of Rats Exposed to Chronic Isolation Stress" in Acta Veterinaria, 72, no. 2 (2022):224-234,
https://doi.org/10.2478/acve-2022-0018 . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Pantelić, Marija
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9959
AB  - Data about the dynamics of noradrenaline (NA) transmission, storage and degradation may be very important for understanding the reduction of functional deficiency of NA and maintaining the stability of NA levels in animals with depressive-like behavior treated with lithium. This study aimed to investigate the effects of mood stabilizer lithium on concentrations of NA in the prefrontal cortex (PFC), as well as behavior rats exposed to chronic restraint stress (CRS). In addition, this study examined the effects of lithium on protein levels of noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and catechol-O-methyltransferase (COMT), as well as the enzyme activity of monoamine oxidase A (MOA) in the PFC of chronically stressed rats. METHODS: The investigated parameters were quantified by Western blot analysis, CAT Research ELISA kits, and an assay of enzyme activity. Also, the forced swim test (FST) was used to assess the behavior of animals. RESULTS: We found that lithium treatment decreased high protein levels of NET and VMAT2, as well as the enzyme activity of MOA in chronically stressed rats to the levels found in unstressed animals. In addition, lithium treatment decreased the concentration of NA (24%) and immobility in animals exposed to CRS. CONCLUSION: Our data confirm that lithium-induced modulation of prefrontal noradrenergic turnover and stabilized the behavior of chronically stressed rats.
T2  - Neuroendocrinology Letters
T1  - Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats
VL  - 42
IS  - 3
SP  - 171
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9959
ER  - 

@article{
author = "Gavrilović, Ljubica and Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pantelić, Marija and Pajović, Snežana B.",
year = "2021",
abstract = "Data about the dynamics of noradrenaline (NA) transmission, storage and degradation may be very important for understanding the reduction of functional deficiency of NA and maintaining the stability of NA levels in animals with depressive-like behavior treated with lithium. This study aimed to investigate the effects of mood stabilizer lithium on concentrations of NA in the prefrontal cortex (PFC), as well as behavior rats exposed to chronic restraint stress (CRS). In addition, this study examined the effects of lithium on protein levels of noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and catechol-O-methyltransferase (COMT), as well as the enzyme activity of monoamine oxidase A (MOA) in the PFC of chronically stressed rats. METHODS: The investigated parameters were quantified by Western blot analysis, CAT Research ELISA kits, and an assay of enzyme activity. Also, the forced swim test (FST) was used to assess the behavior of animals. RESULTS: We found that lithium treatment decreased high protein levels of NET and VMAT2, as well as the enzyme activity of MOA in chronically stressed rats to the levels found in unstressed animals. In addition, lithium treatment decreased the concentration of NA (24%) and immobility in animals exposed to CRS. CONCLUSION: Our data confirm that lithium-induced modulation of prefrontal noradrenergic turnover and stabilized the behavior of chronically stressed rats.",
journal = "Neuroendocrinology Letters",
title = "Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats",
volume = "42",
number = "3",
pages = "171-176",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9959"
}

Gavrilović, L., Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I., Pantelić, M.,& Pajović, S. B.. (2021). Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats. in Neuroendocrinology Letters, 42(3), 171-176.
https://hdl.handle.net/21.15107/rcub_vinar_9959

Gavrilović L, Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Pantelić M, Pajović SB. Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats. in Neuroendocrinology Letters. 2021;42(3):171-176.
https://hdl.handle.net/21.15107/rcub_vinar_9959 .

Gavrilović, Ljubica, Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pantelić, Marija, Pajović, Snežana B., "Effects of mood stabilizer lithium on noradrenergic turnover in the prefrontal cortex of chronically stressed rats" in Neuroendocrinology Letters, 42, no. 3 (2021):171-176,
https://hdl.handle.net/21.15107/rcub_vinar_9959 .

TY  - CONF
AU  - Đorđević, Neda
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Pejić, Snežana
AU  - Tešević, V.
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12583
AB  - Melanoma is responsible for 75% of all deaths from skin cancer. Its lethality arises from its rapid progression, easy metastasis and drug-resistance as well. Red wine is a natural product rich in polyphenolic compounds with potent anticancer activities. It seems that in cancer cells these compounds behave as prooxidants initiating reactive oxygen species mediated cellular DNA breakage and consequent cell death. The aim of this study was to investigate prooxidative activity of red wine samples (Merlot variety, commercial as well as VCR1 and VCR101 clonal wines) in melanoma A375 cells, through measuring the relationship of reduced and oxidized form of glutathione (GSH/GSSG) and comparison with the GSH/GSSG ratio in control (untreated melanoma cells). The data obtained showed that tested red wine samples decrease GSH/GSSG ratio in A375 cells compared to control (4.6 ± 0), with the largest decrease noticed in treatment with VCR101 wine (0.66 ± 0.05).
PB  - The Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume II, September 20-24
T1  - Examination of Prooxidative Activity of Red Wine in Melanoma Cells
SP  - 699
EP  - 702
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12583
ER  - 

@conference{
author = "Đorđević, Neda and Korićanac, Lela and Žakula, Jelena and Todorović Vukotić, Nevena and Pejić, Snežana and Tešević, V. and Pajović, Snežana B.",
year = "2021",
abstract = "Melanoma is responsible for 75% of all deaths from skin cancer. Its lethality arises from its rapid progression, easy metastasis and drug-resistance as well. Red wine is a natural product rich in polyphenolic compounds with potent anticancer activities. It seems that in cancer cells these compounds behave as prooxidants initiating reactive oxygen species mediated cellular DNA breakage and consequent cell death. The aim of this study was to investigate prooxidative activity of red wine samples (Merlot variety, commercial as well as VCR1 and VCR101 clonal wines) in melanoma A375 cells, through measuring the relationship of reduced and oxidized form of glutathione (GSH/GSSG) and comparison with the GSH/GSSG ratio in control (untreated melanoma cells). The data obtained showed that tested red wine samples decrease GSH/GSSG ratio in A375 cells compared to control (4.6 ± 0), with the largest decrease noticed in treatment with VCR101 wine (0.66 ± 0.05).",
publisher = "The Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume II, September 20-24",
title = "Examination of Prooxidative Activity of Red Wine in Melanoma Cells",
pages = "699-702",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12583"
}

Đorđević, N., Korićanac, L., Žakula, J., Todorović Vukotić, N., Pejić, S., Tešević, V.,& Pajović, S. B.. (2021). Examination of Prooxidative Activity of Red Wine in Melanoma Cells. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume II, September 20-24
The Society of Physical Chemists of Serbia., 699-702.
https://hdl.handle.net/21.15107/rcub_vinar_12583

Đorđević N, Korićanac L, Žakula J, Todorović Vukotić N, Pejić S, Tešević V, Pajović SB. Examination of Prooxidative Activity of Red Wine in Melanoma Cells. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume II, September 20-24. 2021;:699-702.
https://hdl.handle.net/21.15107/rcub_vinar_12583 .

Đorđević, Neda, Korićanac, Lela, Žakula, Jelena, Todorović Vukotić, Nevena, Pejić, Snežana, Tešević, V., Pajović, Snežana B., "Examination of Prooxidative Activity of Red Wine in Melanoma Cells" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume II, September 20-24 (2021):699-702,
https://hdl.handle.net/21.15107/rcub_vinar_12583 .

TY  - CONF
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Đorđević, Neda
AU  - Todorović Vukotić, Nevena
AU  - Miletić, J.
AU  - Pejić, Snežana
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12580
AB  - The literature emphasizes the involvement of oxidative stress in the etiopathogenesis of many uterine diseases. Antioxidant system (AOS) represents the protective mechanism used by cells to neutralize overproduced reactive oxygen species (ROS) and prevent oxidative stress. We have previously shown that in gynecological patients with various diagnoses, the reproductive and other factors may be associated with antioxidant capacity and the ability to defend against oxidative damage. In this study, we examined the changes in expression of antioxidant enzymes (AOE): superoxide dismutases (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the blood of women with endometrial hyperplasia. Our results indicate that hyperplasia induces perturbance in oxidative balance, particularly in glutathione redox cycle enzymes.
PB  - The Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24
T1  - Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia
SP  - 308
EP  - 311
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12580
ER  - 

@conference{
author = "Todorović, Ana and Drakulić, Dunja and Gavrilović, Ljubica and Stojiljković, Vesna and Popović, Nataša M. and Đorđević, Neda and Todorović Vukotić, Nevena and Miletić, J. and Pejić, Snežana and Pajović, Snežana B.",
year = "2021",
abstract = "The literature emphasizes the involvement of oxidative stress in the etiopathogenesis of many uterine diseases. Antioxidant system (AOS) represents the protective mechanism used by cells to neutralize overproduced reactive oxygen species (ROS) and prevent oxidative stress. We have previously shown that in gynecological patients with various diagnoses, the reproductive and other factors may be associated with antioxidant capacity and the ability to defend against oxidative damage. In this study, we examined the changes in expression of antioxidant enzymes (AOE): superoxide dismutases (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the blood of women with endometrial hyperplasia. Our results indicate that hyperplasia induces perturbance in oxidative balance, particularly in glutathione redox cycle enzymes.",
publisher = "The Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24",
title = "Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia",
pages = "308-311",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12580"
}

Todorović, A., Drakulić, D., Gavrilović, L., Stojiljković, V., Popović, N. M., Đorđević, N., Todorović Vukotić, N., Miletić, J., Pejić, S.,& Pajović, S. B.. (2021). Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24
The Society of Physical Chemists of Serbia., 308-311.
https://hdl.handle.net/21.15107/rcub_vinar_12580

Todorović A, Drakulić D, Gavrilović L, Stojiljković V, Popović NM, Đorđević N, Todorović Vukotić N, Miletić J, Pejić S, Pajović SB. Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24. 2021;:308-311.
https://hdl.handle.net/21.15107/rcub_vinar_12580 .

Todorović, Ana, Drakulić, Dunja, Gavrilović, Ljubica, Stojiljković, Vesna, Popović, Nataša M., Đorđević, Neda, Todorović Vukotić, Nevena, Miletić, J., Pejić, Snežana, Pajović, Snežana B., "Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24 (2021):308-311,
https://hdl.handle.net/21.15107/rcub_vinar_12580 .

TY  - JOUR
AU  - Todorović Vukotić, Nevena
AU  - Đorđević, Jelena
AU  - Pejić, Snežana
AU  - Đorđević, Neda O.
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9078
AB  - Drug-induced liver injury (DILI) is a serious health burden. It has diverse clinical presentations that can escalate to acute liver failure. The worldwide increase in the use of psychotropic drugs, their long-term use on a daily basis, common comorbidities of psychiatric and metabolic disorders, and polypharmacy in psychiatric patients increase the incidence of psychotropics-induced DILI. During the last 2 decades, hepatotoxicity of various antidepressants (ADs) and antipsychotics (APs) received much attention. Comprehensive review and discussion of accumulated literature data concerning this issue are performed in this study, as hepatotoxic effects of most commonly prescribed ADs and APs are classified, described, and discussed. The review focuses on ADs and APs characterized by the risk of causing liver damage and highlights the ones found to cause life-threatening or severe DILI cases. In parallel, an overview of hepatic oxidative stress, inflammation, and steatosis underlying DILI is provided, followed by extensive review and discussion of the pathophysiology of AD- and AP-induced DILI revealed in case reports, and animal and in vitro studies. The consequences of some ADs and APs ability to affect drug-metabolizing enzymes and therefore provoke drug–drug interactions are also addressed. Continuous collecting of data on drugs, mechanisms, and risk factors for DILI, as well as critical data reviewing, is crucial for easier DILI diagnosis and more efficient risk assessment of AD- and AP-induced DILI. Higher awareness of ADs and APs hepatotoxicity is the prerequisite for their safe use and optimal dosing. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
T2  - Archives of Toxicology
T1  - Antidepressants- and antipsychotics-induced hepatotoxicity
VL  - 95
IS  - 3
SP  - 767
EP  - 789
DO  - 10.1007/s00204-020-02963-4
ER  - 

@article{
author = "Todorović Vukotić, Nevena and Đorđević, Jelena and Pejić, Snežana and Đorđević, Neda O. and Pajović, Snežana B.",
year = "2021",
abstract = "Drug-induced liver injury (DILI) is a serious health burden. It has diverse clinical presentations that can escalate to acute liver failure. The worldwide increase in the use of psychotropic drugs, their long-term use on a daily basis, common comorbidities of psychiatric and metabolic disorders, and polypharmacy in psychiatric patients increase the incidence of psychotropics-induced DILI. During the last 2 decades, hepatotoxicity of various antidepressants (ADs) and antipsychotics (APs) received much attention. Comprehensive review and discussion of accumulated literature data concerning this issue are performed in this study, as hepatotoxic effects of most commonly prescribed ADs and APs are classified, described, and discussed. The review focuses on ADs and APs characterized by the risk of causing liver damage and highlights the ones found to cause life-threatening or severe DILI cases. In parallel, an overview of hepatic oxidative stress, inflammation, and steatosis underlying DILI is provided, followed by extensive review and discussion of the pathophysiology of AD- and AP-induced DILI revealed in case reports, and animal and in vitro studies. The consequences of some ADs and APs ability to affect drug-metabolizing enzymes and therefore provoke drug–drug interactions are also addressed. Continuous collecting of data on drugs, mechanisms, and risk factors for DILI, as well as critical data reviewing, is crucial for easier DILI diagnosis and more efficient risk assessment of AD- and AP-induced DILI. Higher awareness of ADs and APs hepatotoxicity is the prerequisite for their safe use and optimal dosing. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.",
journal = "Archives of Toxicology",
title = "Antidepressants- and antipsychotics-induced hepatotoxicity",
volume = "95",
number = "3",
pages = "767-789",
doi = "10.1007/s00204-020-02963-4"
}

Todorović Vukotić, N., Đorđević, J., Pejić, S., Đorđević, N. O.,& Pajović, S. B.. (2021). Antidepressants- and antipsychotics-induced hepatotoxicity. in Archives of Toxicology, 95(3), 767-789.
https://doi.org/10.1007/s00204-020-02963-4

Todorović Vukotić N, Đorđević J, Pejić S, Đorđević NO, Pajović SB. Antidepressants- and antipsychotics-induced hepatotoxicity. in Archives of Toxicology. 2021;95(3):767-789.
doi:10.1007/s00204-020-02963-4 .

Todorović Vukotić, Nevena, Đorđević, Jelena, Pejić, Snežana, Đorđević, Neda O., Pajović, Snežana B., "Antidepressants- and antipsychotics-induced hepatotoxicity" in Archives of Toxicology, 95, no. 3 (2021):767-789,
https://doi.org/10.1007/s00204-020-02963-4 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Pejić, Snežana
AU  - Grigorov, Ilijana
AU  - Nikolić, Gorana
AU  - Mitić-Ćulafić, Dragana
AU  - Dragićević, Milan
AU  - Đorđević, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Đorđević, Neda O.
AU  - Todorović, Ana
AU  - Drakulić, Dunja R.
AU  - Veljković, Filip M.
AU  - Pajović, Snežana B.
AU  - Todorović, Zoran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9831
AB  - Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.
T2  - Antioxidants
T1  - Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes
VL  - 10
IS  - 6
SP  - 911
DO  - 10.3390/antiox10060911
ER  - 

@article{
author = "Đurašević, Siniša and Pejić, Snežana and Grigorov, Ilijana and Nikolić, Gorana and Mitić-Ćulafić, Dragana and Dragićević, Milan and Đorđević, Jelena and Todorović Vukotić, Nevena and Đorđević, Neda O. and Todorović, Ana and Drakulić, Dunja R. and Veljković, Filip M. and Pajović, Snežana B. and Todorović, Zoran",
year = "2021",
abstract = "Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.",
journal = "Antioxidants",
title = "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes",
volume = "10",
number = "6",
pages = "911",
doi = "10.3390/antiox10060911"
}

Đurašević, S., Pejić, S., Grigorov, I., Nikolić, G., Mitić-Ćulafić, D., Dragićević, M., Đorđević, J., Todorović Vukotić, N., Đorđević, N. O., Todorović, A., Drakulić, D. R., Veljković, F. M., Pajović, S. B.,& Todorović, Z.. (2021). Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants, 10(6), 911.
https://doi.org/10.3390/antiox10060911

Đurašević S, Pejić S, Grigorov I, Nikolić G, Mitić-Ćulafić D, Dragićević M, Đorđević J, Todorović Vukotić N, Đorđević NO, Todorović A, Drakulić DR, Veljković FM, Pajović SB, Todorović Z. Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants. 2021;10(6):911.
doi:10.3390/antiox10060911 .

Đurašević, Siniša, Pejić, Snežana, Grigorov, Ilijana, Nikolić, Gorana, Mitić-Ćulafić, Dragana, Dragićević, Milan, Đorđević, Jelena, Todorović Vukotić, Nevena, Đorđević, Neda O., Todorović, Ana, Drakulić, Dunja R., Veljković, Filip M., Pajović, Snežana B., Todorović, Zoran, "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes" in Antioxidants, 10, no. 6 (2021):911,
https://doi.org/10.3390/antiox10060911 . .

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Pejić, Snežana
AU  - Radojević-Škodrić, Sanja
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Basta-Jovanović, Gordana
AU  - Džamić, Zoran
AU  - Pajović, Snežana B.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8405
AB  - Introduction The oxidative stress contributes to all three phases of carcinogenesis and represents a concomitant condition in renal cell carcinoma (RCC). RCC is the most common type of neoplasm of the kidney, and despite numerous studies the set of predictive and prognostic markers of survival are still unknown. The aim of our study was to examine the relation between antioxidant (AO) status and overall survival (OS) in RCC patients.  Material and methods Our study included 95 patients with RCC, who underwent radical nephrectomy. We analysed the prognostic role of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, glutathione, and malondialde-hyde) and other clinicopathological factors (size, grade, stage, and histological subtype) on the OS of RCC patients.  Results The 5-year OS was 54.6%. The survival analysis related to AO parameters showed no significant difference in survival of RCC patients. The concentration of malondialdehyde, an indicator of lipid peroxidation, also had no significant effect on the survival rate of RCC pa-tients. Univariate and multivariate analysis confirmed the significance of clinicopathological parameters (size, p < 0.001; Fuhrman grade, p = 0.001, and stage, p < 0.001) for patients’ survival.  Conclusions In our cohort of patients, different antioxidant parameters were not found to be predictors for OS of patients with RCC, who underwent radical nephrectomy.
T2  - Archives of Medical Science
T1  - The effect of antioxidant status on overall survival in renal cell carcinoma
VL  - 16
IS  - 1
SP  - 94
EP  - 101
DO  - 10.5114/aoms.2019.86818
ER  - 

@article{
author = "Pavlović, Ivan and Pejić, Snežana and Radojević-Škodrić, Sanja and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Basta-Jovanović, Gordana and Džamić, Zoran and Pajović, Snežana B.",
year = "2020",
abstract = "Introduction The oxidative stress contributes to all three phases of carcinogenesis and represents a concomitant condition in renal cell carcinoma (RCC). RCC is the most common type of neoplasm of the kidney, and despite numerous studies the set of predictive and prognostic markers of survival are still unknown. The aim of our study was to examine the relation between antioxidant (AO) status and overall survival (OS) in RCC patients.  Material and methods Our study included 95 patients with RCC, who underwent radical nephrectomy. We analysed the prognostic role of antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, glutathione, and malondialde-hyde) and other clinicopathological factors (size, grade, stage, and histological subtype) on the OS of RCC patients.  Results The 5-year OS was 54.6%. The survival analysis related to AO parameters showed no significant difference in survival of RCC patients. The concentration of malondialdehyde, an indicator of lipid peroxidation, also had no significant effect on the survival rate of RCC pa-tients. Univariate and multivariate analysis confirmed the significance of clinicopathological parameters (size, p < 0.001; Fuhrman grade, p = 0.001, and stage, p < 0.001) for patients’ survival.  Conclusions In our cohort of patients, different antioxidant parameters were not found to be predictors for OS of patients with RCC, who underwent radical nephrectomy.",
journal = "Archives of Medical Science",
title = "The effect of antioxidant status on overall survival in renal cell carcinoma",
volume = "16",
number = "1",
pages = "94-101",
doi = "10.5114/aoms.2019.86818"
}

Pavlović, I., Pejić, S., Radojević-Škodrić, S., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M., Basta-Jovanović, G., Džamić, Z.,& Pajović, S. B.. (2020). The effect of antioxidant status on overall survival in renal cell carcinoma. in Archives of Medical Science, 16(1), 94-101.
https://doi.org/10.5114/aoms.2019.86818

Pavlović I, Pejić S, Radojević-Škodrić S, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Basta-Jovanović G, Džamić Z, Pajović SB. The effect of antioxidant status on overall survival in renal cell carcinoma. in Archives of Medical Science. 2020;16(1):94-101.
doi:10.5114/aoms.2019.86818 .

Pavlović, Ivan, Pejić, Snežana, Radojević-Škodrić, Sanja, Todorović, Ana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Basta-Jovanović, Gordana, Džamić, Zoran, Pajović, Snežana B., "The effect of antioxidant status on overall survival in renal cell carcinoma" in Archives of Medical Science, 16, no. 1 (2020):94-101,
https://doi.org/10.5114/aoms.2019.86818 . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9617
AB  - This study aimed to investigate the effects of chronic restraint stress (CRS) on the protein levels of dopamine-β-hydroxylase (DBH), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and brain-derived neurotrophic factor (BDNF), as well as the concentration of noradrenaline (NA) in the rat hippocampus. The investigated parameters were quantified by Western blot analyses and ELISA kits. We found that CRS increased the protein levels of DBH by 30 %, VMAT2 by 11 %, BDNF by 11 % and the concentration of NA by 104 %, but decreased the protein levels of NET by 16 % in the hippocampus of chronically stressed rats. The molecular mechanisms by which CRS increased the hippocampal NA level are an important adaptive phenomenon of the noradrenergic system in the stress condition. © 2020 Charles University. All rights reserved.
T2  - Folia Biologica (Czech Republic)
T1  - Changes of Hippocampal Noradrenergic Capacity in Stress Condition
VL  - 66
IS  - 2
SP  - 81
EP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9617
ER  - 

@article{
author = "Gavrilović, Ljubica and Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pajović, Snežana B.",
year = "2020",
abstract = "This study aimed to investigate the effects of chronic restraint stress (CRS) on the protein levels of dopamine-β-hydroxylase (DBH), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and brain-derived neurotrophic factor (BDNF), as well as the concentration of noradrenaline (NA) in the rat hippocampus. The investigated parameters were quantified by Western blot analyses and ELISA kits. We found that CRS increased the protein levels of DBH by 30 %, VMAT2 by 11 %, BDNF by 11 % and the concentration of NA by 104 %, but decreased the protein levels of NET by 16 % in the hippocampus of chronically stressed rats. The molecular mechanisms by which CRS increased the hippocampal NA level are an important adaptive phenomenon of the noradrenergic system in the stress condition. © 2020 Charles University. All rights reserved.",
journal = "Folia Biologica (Czech Republic)",
title = "Changes of Hippocampal Noradrenergic Capacity in Stress Condition",
volume = "66",
number = "2",
pages = "81-84",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9617"
}

Gavrilović, L., Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I.,& Pajović, S. B.. (2020). Changes of Hippocampal Noradrenergic Capacity in Stress Condition. in Folia Biologica (Czech Republic), 66(2), 81-84.
https://hdl.handle.net/21.15107/rcub_vinar_9617

Gavrilović L, Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Pajović SB. Changes of Hippocampal Noradrenergic Capacity in Stress Condition. in Folia Biologica (Czech Republic). 2020;66(2):81-84.
https://hdl.handle.net/21.15107/rcub_vinar_9617 .

Gavrilović, Ljubica, Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pajović, Snežana B., "Changes of Hippocampal Noradrenergic Capacity in Stress Condition" in Folia Biologica (Czech Republic), 66, no. 2 (2020):81-84,
https://hdl.handle.net/21.15107/rcub_vinar_9617 .

TY  - JOUR
AU  - Senćanski, Milan V.
AU  - Perović, Vladimir R.
AU  - Pajović, Snežana B.
AU  - Adžić, Miroslav
AU  - Paessler, Slobodan
AU  - Glišić, Sanja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9611
AB  - The SARS-CoV-2 outbreak caused an unprecedented global public health threat, having a high transmission rate with currently no drugs or vaccines approved. An alternative powerful additional approach to counteract COVID-19 is in silico drug repurposing. The SARS-CoV-2 main protease is essential for viral replication and an attractive drug target. In this study, we used the virtual screening protocol with both long-range and short-range interactions to select candidate SARS-CoV-2 main protease inhibitors. First, the Informational spectrum method applied for small molecules was used for searching the Drugbank database and further followed by molecular docking. After in silico screening of drug space, we identified 57 drugs as potential SARS-CoV-2 main protease inhibitors that we propose for further experimental testing. © 2020 by the authors.
T2  - Molecules
T1  - Drug Repurposing for Candidate SARS-CoV-2 Main Protease Inhibitors by a Novel in Silico Method
VL  - 25
IS  - 17
DO  - 10.3390/molecules25173830
ER  - 

@article{
author = "Senćanski, Milan V. and Perović, Vladimir R. and Pajović, Snežana B. and Adžić, Miroslav and Paessler, Slobodan and Glišić, Sanja",
year = "2020",
abstract = "The SARS-CoV-2 outbreak caused an unprecedented global public health threat, having a high transmission rate with currently no drugs or vaccines approved. An alternative powerful additional approach to counteract COVID-19 is in silico drug repurposing. The SARS-CoV-2 main protease is essential for viral replication and an attractive drug target. In this study, we used the virtual screening protocol with both long-range and short-range interactions to select candidate SARS-CoV-2 main protease inhibitors. First, the Informational spectrum method applied for small molecules was used for searching the Drugbank database and further followed by molecular docking. After in silico screening of drug space, we identified 57 drugs as potential SARS-CoV-2 main protease inhibitors that we propose for further experimental testing. © 2020 by the authors.",
journal = "Molecules",
title = "Drug Repurposing for Candidate SARS-CoV-2 Main Protease Inhibitors by a Novel in Silico Method",
volume = "25",
number = "17",
doi = "10.3390/molecules25173830"
}

Senćanski, M. V., Perović, V. R., Pajović, S. B., Adžić, M., Paessler, S.,& Glišić, S.. (2020). Drug Repurposing for Candidate SARS-CoV-2 Main Protease Inhibitors by a Novel in Silico Method. in Molecules, 25(17).
https://doi.org/10.3390/molecules25173830

Senćanski MV, Perović VR, Pajović SB, Adžić M, Paessler S, Glišić S. Drug Repurposing for Candidate SARS-CoV-2 Main Protease Inhibitors by a Novel in Silico Method. in Molecules. 2020;25(17).
doi:10.3390/molecules25173830 .

Senćanski, Milan V., Perović, Vladimir R., Pajović, Snežana B., Adžić, Miroslav, Paessler, Slobodan, Glišić, Sanja, "Drug Repurposing for Candidate SARS-CoV-2 Main Protease Inhibitors by a Novel in Silico Method" in Molecules, 25, no. 17 (2020),
https://doi.org/10.3390/molecules25173830 . .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
AU  - Pajović, Snežana B.
PY  - 2019
UR  - https://www.hindawi.com/journals/omcl/2019/8745376/
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8085
AB  - This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium
VL  - 2019
SP  - 1
EP  - 11
DO  - 10.1155/2019/8745376
ER  - 

@article{
author = "Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Gavrilović, Ljubica and Pajović, Snežana B.",
year = "2019",
abstract = "This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium",
volume = "2019",
pages = "1-11",
doi = "10.1155/2019/8745376"
}

Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I., Gavrilović, L.,& Pajović, S. B.. (2019). Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium. in Oxidative Medicine and Cellular Longevity, 2019, 1-11.
https://doi.org/10.1155/2019/8745376

Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Gavrilović L, Pajović SB. Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium. in Oxidative Medicine and Cellular Longevity. 2019;2019:1-11.
doi:10.1155/2019/8745376 .

Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Gavrilović, Ljubica, Pajović, Snežana B., "Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium" in Oxidative Medicine and Cellular Longevity, 2019 (2019):1-11,
https://doi.org/10.1155/2019/8745376 . .