@article{
author = "Bogdanović, Gordana and Jurišić, Vladimir and Kraguljac, Nada and Mrđanović, Jasminka Ž. and Jakimov, Dimitar and Krtolica-Žikić, Koviljka and Krajnović, Milena M. and Magic, Zvonko and Stojiljković, Bratislav and Andrijevic, Ljiljana and Srdić, Tatjana and Baltic, Mirjana and Popovic, Stevan",
year = "2007",
abstract = "We report on characteristics of the first human cell line, PC-MDS, derived from a bone marrow of a patient with therapy-related myelodysplastic syndrome (t-MDS) who had no overt post-MDS leukemia. Classic cytology analyses, immunophenotyping, cytogenetic and molecular genetic procedures were used for characterization of the cell line. PC-MDS cells are positive for the expression of CD13, CD15, CD30, CD33, and CD45 antigen. Positive cytochemical staining and immunophenotype analyses indicated that PC-MDS cells have some characteristics of the early myeloid precursor cell. The karyotype analysis of PC-MDS cell line revealed various numerical and structural changes including those typically associated with t-MDS: del(5)(q13)[7], der(5)t(5;11)(p11;q11)[13], -7[6], del(7)(q31)[2], +20[3], -20[4]. Evaluation of methylation status in a promoter region of p 15, p 16 and MGMT genes showed biallelic hypermethylation pattern of 5 promoter region only in MGMT gene. PC-MDS is the first t-MDS derived cell line, and based on its immunological, cytogenetic and molecular characterization could be a new tool in evaluation of complex biology of MDS and a model for methylation studies. (c) 2007 Elsevier Ltd. All rights reserved.",
journal = "Leukemia Research",
title = "Characteristics of novel myeloid precursor cell line, PC-MDS, established from a bone marrow of the patient with therapy-related myelodysplastic syndrome",
volume = "31",
number = "8",
pages = "1097-1105",
doi = "10.1016/j.leukres.2007.01.012"
}