TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Vujović, Predrag
AU  - Pajović, Snežana B.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10235
AB  - Background This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS). Methods The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities. Results We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals. Conclusions These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understanding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress.
T2  - PeerJ
T1  - Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium
VL  - 10
SP  - e13020
DO  - 10.7717/peerj.13020
ER  - 

@article{
author = "Gavrilović, Ljubica and Popović, Nataša and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Vujović, Predrag and Pajović, Snežana B.",
year = "2022",
abstract = "Background This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS). Methods The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities. Results We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals. Conclusions These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understanding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress.",
journal = "PeerJ",
title = "Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium",
volume = "10",
pages = "e13020",
doi = "10.7717/peerj.13020"
}

Gavrilović, L., Popović, N., Stojiljković, V., Pejić, S., Todorović, A., Vujović, P.,& Pajović, S. B.. (2022). Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium. in PeerJ, 10, e13020.
https://doi.org/10.7717/peerj.13020

Gavrilović L, Popović N, Stojiljković V, Pejić S, Todorović A, Vujović P, Pajović SB. Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium. in PeerJ. 2022;10:e13020.
doi:10.7717/peerj.13020 .

Gavrilović, Ljubica, Popović, Nataša, Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Vujović, Predrag, Pajović, Snežana B., "Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium" in PeerJ, 10 (2022):e13020,
https://doi.org/10.7717/peerj.13020 . .

TY  - JOUR
AU  - Vujović, Predrag
AU  - Lakić, Iva
AU  - Jasnić, Nebojša
AU  - Jevđović, Tanja
AU  - Đurašević, Siniša F.
AU  - Isenović, Esma R.
AU  - Đorđević, Jelena
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1120
AB  - Given that both prolactin and galanin take part in the regulation of energy homeostasis and that galanin is localized within lactotrophs, this study was aimed at comparing the pituitary expression patterns of prolactin and galanin during different phases of metabolic response to starvation in adult Wistar male rats. Food was removed at the onset of the dark phase (6:00 pm) and the animals were deprived for 6, 12, 24 and 48 h. Each of the starved groups (n=6) was killed simultaneously with a group of ad libitum-fed rats (n=6), and the intrapituitary levels of prolactin and galanin were examined. Galanin expression in the hypothalamus and the circulating levels of prolactin were also assessed. Starvation induced a rise in the intrapituitary prolactin level (p LT 0.001), whereas the opposite trend was detected in the serum (p LT 0.05). The galanin pituitary level was initially increased (6, 12 h) (p LT 0.05), but as starvation progressed, it first reached (at 24 h) and ultimately fell below the level recorded in the ad libitum rats (at 48 h) (p LT 0.05). Both prolactin and galanin were elevated in the hypothalamus after 24- and 48-h starvation. The results show that the starvation-induced increase in the pituitary prolactin expression did not lead to the rise in prolactin circulating levels, but rather resulted in the elevation of the prolactin hypothalamic content. Furthermore, the results suggest that under the circumstances of disturbed energy homeostasis, galanin might be responsible for the augmented prolactin production, initially at the pituitary and subsequently at the hypothalamic level.
T2  - Archives of Biological Sciences
T1  - Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern
VL  - 68
IS  - 1
SP  - 117
EP  - 123
DO  - 10.2298/ABS150525133V
ER  - 

@article{
author = "Vujović, Predrag and Lakić, Iva and Jasnić, Nebojša and Jevđović, Tanja and Đurašević, Siniša F. and Isenović, Esma R. and Đorđević, Jelena",
year = "2016",
abstract = "Given that both prolactin and galanin take part in the regulation of energy homeostasis and that galanin is localized within lactotrophs, this study was aimed at comparing the pituitary expression patterns of prolactin and galanin during different phases of metabolic response to starvation in adult Wistar male rats. Food was removed at the onset of the dark phase (6:00 pm) and the animals were deprived for 6, 12, 24 and 48 h. Each of the starved groups (n=6) was killed simultaneously with a group of ad libitum-fed rats (n=6), and the intrapituitary levels of prolactin and galanin were examined. Galanin expression in the hypothalamus and the circulating levels of prolactin were also assessed. Starvation induced a rise in the intrapituitary prolactin level (p LT 0.001), whereas the opposite trend was detected in the serum (p LT 0.05). The galanin pituitary level was initially increased (6, 12 h) (p LT 0.05), but as starvation progressed, it first reached (at 24 h) and ultimately fell below the level recorded in the ad libitum rats (at 48 h) (p LT 0.05). Both prolactin and galanin were elevated in the hypothalamus after 24- and 48-h starvation. The results show that the starvation-induced increase in the pituitary prolactin expression did not lead to the rise in prolactin circulating levels, but rather resulted in the elevation of the prolactin hypothalamic content. Furthermore, the results suggest that under the circumstances of disturbed energy homeostasis, galanin might be responsible for the augmented prolactin production, initially at the pituitary and subsequently at the hypothalamic level.",
journal = "Archives of Biological Sciences",
title = "Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern",
volume = "68",
number = "1",
pages = "117-123",
doi = "10.2298/ABS150525133V"
}

Vujović, P., Lakić, I., Jasnić, N., Jevđović, T., Đurašević, S. F., Isenović, E. R.,& Đorđević, J.. (2016). Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern. in Archives of Biological Sciences, 68(1), 117-123.
https://doi.org/10.2298/ABS150525133V

Vujović P, Lakić I, Jasnić N, Jevđović T, Đurašević SF, Isenović ER, Đorđević J. Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern. in Archives of Biological Sciences. 2016;68(1):117-123.
doi:10.2298/ABS150525133V .

Vujović, Predrag, Lakić, Iva, Jasnić, Nebojša, Jevđović, Tanja, Đurašević, Siniša F., Isenović, Esma R., Đorđević, Jelena, "Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern" in Archives of Biological Sciences, 68, no. 1 (2016):117-123,
https://doi.org/10.2298/ABS150525133V . .